Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.56608746
We conclude that c Src protein kinase is associated specifically with the AhR complex along with hsp 90 in the cytosol of these cells and that upon ligand binding to the Ah receptor subunit , c Src is activated and released from the complex . . 0.56608746^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Maturation of the tyrosine kinase c src as a kinase and as a substrate depends on the molecular chaperone Hsp 90 . ^^^ By modulating Hsp 90 levels in Saccharomyces cerevisiae , we determined that Hsp 90 is indeed necessary for the maturation of c src ( the normal homolog of 5 src ) . c src maturation is , however , less sensitive to Hsp 90 perturbations than is 5 src maturation . ^^^ Dependence of the two proteins on Hsp 90 does not correspond to their relative efficiency in reaching their final destination ( the plasma membrane ) ; we observed that in yeast , unlike in vertebrate cells , neither c src nor 5 src concentrate in the membrane . ^^^ Expression of different v / c src chimeras in cells carrying wild type or temperature sensitive Hsp 90 alleles revealed that the difference between the proteins instead arises from multiple , naturally occurring mutations in the C terminal region of 5 src . . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Here , we demonstrate that treatment of the human acute myeloid leukemia HL 60 cells with 17 AAG attenuates the intracellular levels of a number of Hsp 90 client proteins , including Akt , c Raf 1 , and c Src . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Other Hsp 90 regulated proteins , i . e . the phosphokinases raf 1 , lck , and c src , were not affected , indicating a specific functional interference of cisplatin with the steroid receptors GR and androgen receptor . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
The additional molecular targets considered here include the type 1 insulin like growth factor receptor , the epidermal growth factor receptor , mammalian target of rapamycin , NF kappaB , hypoxia inducible factor 1alpha , hsp 90 , cyclooxygenase 2 , protein kinase A type 1 , vascular endothelial growth factor , 5 lipoxygenase , 12 lipoxygenase , angiotensin 2 receptor type 1 , bradykinin receptor type 1 , c Src , interleukin 6 , ras , MDM 2 , bcl 2 / bclxL , vitamin D receptor , estrogen receptor beta , and PPAR . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
MUC 1 oncoprotein is targeted to mitochondria by heregulin induced activation of c Src and the molecular chaperone HSP 90 . ^^^ By contrast , binding of MUC 1 to HSP 90 in vitro is induced by c Src mediated phosphorylation of the MUC 1 cytoplasmic domain . c Src also increases binding of MUC 1 to HSP 90 in cells . ^^^ In concert with these results , we show that heregulin ( HRG ) , a ligand for ErbB receptors , activates c Src and , in turn , stimulates binding of MUC 1 to HSP 90 . ^^^ We also show that inhibitors of c Src or HSP 90 block HRG induced targeting of MUC 1 to mitochondria and integration of MUC 1 into the mitochondrial outer membrane . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Dual purpose molecular targets , whose inhibition could be expected to decrease the aggressiveness and chemoresistance of cancer cells while simultaneously impeding angiogenesis , include NF kappaB , cox 2 , c Src , Stat 3 , and hsp 90 ; drugs that can address these targets are now in development , and salicylates are notable for the fact that they can simultaneously inhibit NF kappaB and cox 2 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
In addition , hsp 90 in the lysate complexes with wild type pp60v src , of which only a small portion is normally recovered in cytosol in the native multiprotein complex , and with the cellular homolog , pp60c src , which has never been recovered in cytosol in the form of a native multiprotein complex with hsp 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
This function of hsp 90 is highly selective for pp60v src : the same hsp 90 mutation has no effect on the activity or specificity of the exogenous pp160v abl tyrosine kinase ; similarly , it does not affect the specificity and has only a very small effect on the activity of the exogenous pp60c src kinase . . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
For all seven protein systems considered ( HIV 1 protease , dihydrodipicolinate reductase , Rnase T 1 , streptavidin , pp60c Src SH 2 domain , Hsp 90 molecular chaperone , and bovine beta trypsin ) the binding enthalpy of 25 small molecular weight peptide and nonpeptide ligands can be accounted for with a standard error of + / 0 . 3 kcal 10 mol ( 1 ) . . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Association of Hsp 90 with cellular Src family kinases in a cell free system correlates with altered kinase structure and function . ^^^ Following synthesis in the cytoplasm , the transforming proteins encoded by the retroviral oncogenes src , yes , fps , fes , and fgr form complexes with hsp 90 and the hsp 90 cohort p 50 . ^^^ In this paper , we have utilized protein synthesis in reticulocyte lysate to determine whether three cellular members of the src family of tyrosine kinases , myeloid specific p59fgr , B cell specific p59fgr , and p56lck , form complexes with hsp 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Inhibition of heat shock protein HSP 90 pp60v src heteroprotein complex formation by benzoquinone ansamycins : essential role for stress proteins in oncogenic transformation . ^^^ Consistent with these binding data , we found that soluble geldanamycin and herbimycin A inhibited specifically the formation of a previously described src HSP 90 heteroprotein complex . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
We demonstrate that Raf 1 purified form Sf 9 cells coinfected with baculovirus Ras but not Src could be inactivated by protein tyrosine phosphatase PTP 1B . 14 3 3 and Hsp 90 proteins blocked both the tyrosine dephosphorylation and inactivation of Raf 1 , suggesting that Raf 1 activity is phosphotyrosine dependent . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Several lines of evidence suggest that members of the 90 kDa family of heat shock proteins ( hsp 90 ) may support the folding of various homologues of the src kinase family . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
We have expressed the mitogenic signaling proteins Src , Ras , Raf 1 , Mek ( MAP kinase kinase ) , and Erk ( MAP kinase ) in baculovirus infected Sf 9 insect cells in order to study a potential role for the chaperone hsp 90 in formation of multiprotein complexes . ^^^ One cellular factor thought to be involved in signaling protein complex formation is the chaperone hsp 90 , and we show that Src , Raf , and Mek are each complexed with insect hsp 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Several protein kinases , including the Src and Raf components of the MAP kinase system , are also bound to hsp 90 . ^^^ Genetic studies in yeast have demonstrated that hsp 90 is required for normal signaling via steroid and dioxin receptors and for the activity of Src in vivo . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Three components of the MAP kinase signalling system ( Src , Raf , and Mek ) exist in complexes with hsp 90 and a 50 kDa protein that is the mammalian homolog of the yeast cell cycle control protein cdc 37 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
The catalytic domain required geldanamycin inhibitable heat shock protein 90 ( hsp 90 ) function to achieve its active protease resistant conformation , but the src homology 2 ( SH 2 ) domain acquired phosphopeptide binding competence independently of hsp 90 function . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Large portions of the GR HBD have been implicated and no similarity has been established between steroid receptor HBDs and the catalytic domains of the protein kinases ( e . g . pp 60 ( src ) , Raf ) that also form stable heterocomplexes with hsp 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Several protein kinases ( e . g . pp 60 ( src ) , 5 Raf ) exist in heterocomplexes with hsp 90 and a 50 kDa protein that is the mammalian homolog of the yeast cell cycle control protein Cdc 37 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
The existence of an estrogen receptor containing , multimeric complex consisting of hsp 90 , src , and B Raf also suggests a direct link between the estrogen receptor and the MAP kinase signaling cascade . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Pretreatment of WB F 344 cells for 1 h with 1 microM geldanamycin , which disrupts cytosolic heat shock protein Hsp 90 complexes with AhR and Src , abolished TCDD mediated Src translocation and TCDD mediated reduction of cell contact inhibition . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
In addition , both Cys483 / Cyr487 and Cys496 / Cys498 kinases became resistant to in vitro inactivation by herbimycin A , which directly inactivates 5 Src in addition to its effect on HSP 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Hsp 90 is essential for the synthesis and subsequent membrane association , but not the maintenance , of the Src kinase p 56 ( lck ) . ^^^ Here , we have studied the involvement of Hsp 90 in the synthesis , membrane binding , and maintenance of the Src kinase Lck . ^^^ In contrast , LckY505F and LckDeltaSH 2 , constitutively active Lck mutants lacking the C terminal regulatory tyrosine or the entire Src homology 2 domain , respectively , required Hsp 90 activity to stabilize the mature proteins . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
The Cdc 37 gene encodes a 50 kDa protein which targets intrinsically unstable oncoprotein kinases such as Cdk 4 , Raf 1 , and src to the molecular chaperone Hsp 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
HSP 90 in particular , but also HSP 70 and other chaperones associate with proteins of the mitogen activated signal cascade , particularly with the Src kinase , with tyrosine receptor kinases , with Raf and the MAP kinase activating kinase ( MEK ) . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
In addition , UCS15A appeared to differ from src destabilizing agents such as herbimycin and radicicol that destabilize src by interfering with Hsp 90 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
FLAG Harc binds Hsp 90 but unlike Cdc 37 does not bind Src family kinases or Raf 1 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Because HSP 90 is essential for the activities of Src family kinases , these results suggest that localization of N WASP modulates Src kinase activity by regulating HSP 90 expression . . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
In 1994 , these ansamycins were found to bind to Hsp 90 and to cause the degradation of client proteins including Src kinases ; further efforts to develop anticancer drugs were made using geldanamycin analogs , and 17AAG was chosen as the best candidate for clinical trials . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Heat shock protein 90 ( HSP 90 ) is involved in the folding of proteins such as signal transduction molecules ( Src , Raf 1 , cdk 4 ) and steroid receptors and in enhancing the activity of telomerase and nitric oxide synthase . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Regulation of the Src family kinase Lck by Hsp 90 and ubiquitination . ^^^ By binding to the ATP binding site , the Src family inhibitor PP 2 reduced ubiquitination and overcame the need for Hsp 90 monitoring of active Lck . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
In this report , the crystal structure of the Src family tyrosine kinase Lck was used to guide the creation of kinase constructs to determine features recognized by Hsp 90 and its `` kinase specific ' ' co chaperone Cdc 37 . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
HSP 90 increases the phosphorylation of N WASP by 5 Src , leading to enhanced N WASP dependent actin polymerization . ^^^ HSP 90 is colocalized and associated with active N WASP at podosomes in 3Y1 / v Src cells and at growing neurites in PC 12 cells , whose actin structures are clearly inhibited by blocking the binding of HSP 90 to N WASP . ^^^ These findings suggest that HSP 90 induces efficient activation of N WASP downstream of phosphorylation signal by Src family kinases and is critical for N WASP dependent podosome formation and neurite extension . . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Purine based compounds have found new applications as inducers of interferon and lineage committed cell dedifferentiation , agonists and antagonists of adenosine receptors , ligands of corticotropin releasing hormone receptors , and as inhibitors of HSP 90 , Src kinase , p38alpha MAP kinase , sulfotransferases , phosphodiesterases , and Cdks . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
Hsp 90 inhibition transiently activates Src kinase and promotes Src dependent Akt and Erk activation . ^^^ Here , we show that the prototypical Hsp 90 inhibitor geldanamycin induces Akt and Erk activation that is independent of PTEN status and is mediated by transient activation of Src kinase . ^^^ We show that geldanamycin rapidly disrupts Src association with Hsp 90 , suggesting that Src activation results directly from dissociation of the chaperone . ^^^ These data suggest that , under certain circumstances , dual inhibition of Hsp 90 and Src may be warranted . . ^^^
Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P07900	Pubmed	SVM Score :0.0
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