Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P12931 and P03372	Pubmed	SVM Score :1.2137501
We then demonstrate that E 2 enhances interactions between ERalpha and the RIDs of SRC 1 and SRC 3 , whereas the interaction between ERalpha with the SRC 2 RID is ligand independent . 1.2137501^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.51319488
Oestradiol treatment of MCF 7 cells triggers simultaneous association of ER with Src and p 85 , the regulatory subunit of phosphatidylinositol 3 kinase ( PI 3 kinase ) and activation of Src and PI 3 K dependent pathways . 0.51319488^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.51341541
ER interacts with Src ' s SH 2 domain using phosphotyrosine 537 , and this complex was further stabilized by MNAR ER interaction . 0.51341541^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.84492864
Herein we utilize a library of SRC 2 peptidomimetics to select for specific inhibitors of the interaction of SRC 2 with the two estrogen receptor ( ER ) isoforms , ERalpha and ERbeta , in the presence of three different ligands : 17beta estradiol , diethylstilbesterol , and genistein . 0.84492864^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.99098951
Whereas wild type ER showed interaction with SRC 1 only in the presence of estrogen , Y537A and Y537S ER showed moderate or full interaction in the absence of ligand , an interaction that was blocked by antiestrogen , and the magnitude of interaction was increased to or remained at 100 % upon estradiol treatment , implying that the ability of an ER to associate with SRC 1 is a good indicator of a transcriptionally active conformational state of the receptor . 0.99098951^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.60108402
CONCLUSIONS : 1 ) FRET based coactivator association is a novel approach for characterizing nuclear receptor agonists or antagonists ; individual ligands display potencies that are predictive of in vivo effects and distinct profiles of maximal activity that are suggestive of alternative receptor conformations . 2 ) PPARgamma interacts with both CBP and SRC 1 ; transcriptional activation and coactivator association are AF 2 dependent . 3 ) Nuclear receptor LBDs have distinct affinities for individual coactivators ; thus , PPARgamma has a greater apparent affinity for CBP than for SRC 1 , whereas ERalpha interacts preferentially with SRC 1 but very weakly with CBP . . 0.60108402^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.94242807
SRC 1 , AIB 1 , and RIP 140 interacted constitutively with the L536P ER , whereas TIF 1 and TIF 2 interacted only weakly in the absence of hormone . 0.94242807^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.55466822
Enhancement of the interaction between SRC 1 and ER beta AF 1 is also observed in vivo in cells either treated with EGF or expressing activated Ras . 0.55466822^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.67290845
Thus , in oestrogenic conditions SRC 1 preferentially binds to the ER which effectively sequesters it thereby reducing enhancer activity , but in antioestrogenic media the cofactor is released from the ER and is therefore available to activate the ERBB 2 enhancer . . 0.67290845^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.79465578
Accordingly , ERalpha dephosphorylation decreases its ligand independent interaction with SRC 1 and CBP in vitro . 0.79465578^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :1.0966839
Basal and estradiol ( E 2 ) dependent interactions between the ERalpha ligand binding domain ( LBD ) and SRC 1 , TIF 2 or RAC 3 were observed in HeLa and HepG 2 cells . 1.0966839^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :1.2724379
Competition experiments of the complex between ERalpha and fluorescently labelled PGC 1 91 408 with unlabelled SRC 1 570 780 showed that PGC 1 91 408 was an efficient competitor of SRC 1 570 780 , while the inverse was not true , underscoring their distinct modes of binding . 1.2724379^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
With this in mind , the 221 residue long influenza virus hemagglutinin 2 ( IVHA 2 ) , as a representative of alien antigens , was compared with three diverse proteins representing the host : 533 residue long chicken c src protein kinase ( c src product of the cellular oncogene of Rous sarcoma virus ) , 595 residue long human estrogen receptor , and 585 residue long human serum albumin . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Transfection experiments of Cos cells with the estradiol receptor cDNA and in vitro experiments with c src show that the estradiol receptor activates c src and this activation requires occupancy of the receptor by hormone . ^^^ Our experiments suggest that c src is an initial and integral part of the signaling events mediated by the estradiol receptor . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Our findings show that in subconfluent Caco 2 cells , the estradiol receptor complex activates the c src , c yes / MAP kinase pathway and activates growth . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
It has been reported previously that estrogens activate the signal transducing Src / p21 ( ras ) / Erk pathway in human breast cancer cells via an interaction of estrogen receptor ( ER ) with c Src . ^^^ PRB does not interact with c Src but associates via the N terminal 168 amino acids with ER . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Phosphorylation of the EGF receptor by c SRC is also critical for mitogenic signaling initiated by the EGF receptor itself , as well as by several G protein coupled receptors ( GPCRs ) , a cytokine receptor , and the estrogen receptor . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The present studies show for the first time in the brain that E 2 elicits phosphorylation of c Src on three functionally critical tyrosine residues ( Y 220 , Y 423 , and Y 534 ) , and that this phosphorylation occurs despite disruption of ER alpha ( in ER knockout mice ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
A 17beta estradiol induced estrogen receptor / c Src association correlated with rapid c Src phosphorylation . ^^^ Estrogen rapidly activated c Src inducing an estrogen receptor , c Src , and P 85 ( regulatory subunit of PI 3 kinase ) complex formation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
However , neither inhibitors nor inducers of extracellular signal regulated kinases 1 and 2 ( ERK1 / 2 ) , phosphatidylinositol 3 kinase , protein kinase C , c Src , or protein kinase A modified ER mediated activity in this model . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In breast cancer cells , estrogens activate the Src / Erk pathway through an interaction of the estrogen receptor alpha ( ERalpha ) with the SH 2 domain of c Src . ^^^ Progestins have been reported to activate also this pathway either via an interaction of the progesterone receptor isoform B ( PRB ) with ERalpha , which itself activates c Src , or by direct interaction of PRB with the SH 3 domain of c Src . ^^^ Mutations in the proline cluster of PRB that prevent a direct interaction with c Src do not affect the strong activation of c Src by progestins in the presence of ERalpha . ^^^ Two domains of the progesterone receptor interact with the estrogen receptor and are required for progesterone activation of the c Src / Erk pathway in mammalian cells . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In contrast , negligible membrane translocation of PKCalpha and / or activated c Src was observed in parental ROS 17 / 2 . 8 cells , which express low levels of full length ERalpha . ^^^ ERalpha co immunoprecipitated c Src and PKCalpha , mostly in its cleaved form ( PKMalpha ) . ^^^ These data highlight direct PKCalpha c Src ERalpha interactions , which may be crucial in the modulation of estrogen responsiveness and the differentiation process in osteoblasts . . ^^^ Interaction of estrogen receptor alpha with protein kinase C alpha and c Src in osteoblasts during differentiation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Fibronectin and type 4 collagen activate ERalpha AF 1 by c Src pathway : effect on breast cancer cell motility . ^^^ It is worth noting that c Src activation is coincident with adhesion of cells to ECM proteins and that the inhibition of c Src activity by PP 2 or the expression of a dominant negative c Src abolishes ERalpha mediated transcription and partially reverts the inhibition of cell invasiveness in ERalpha positive cancer cells . ^^^ These findings address the integrated role of ECM proteins and ERalpha in influencing breast cancer cell motility through a mechanism that involves c Src and seems not to be related to a specific cell type . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Our studies provide a molecular link that connects the c Src tyrosine kinase transduction pathway to ER stress induced transcriptional activation of Grp 78 mediated by TFII I . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We also describe the rapid assembly of a membrane associated molecular complex , comprised of ER , c Src and the regulatory unit of phosphatidylinositol 3 kinase ( PI3K ) , p 85 , in response to estrogen . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Downregulation of cardiac Kv4 . 3 transcripts was mimicked by estrogen treatment in ovariectomized mice , and was prevented by the estrogen receptor antagonist ICI 182 , 780 . c Src activity increased by approximately 2 fold in late pregnancy and after estrogen treatment . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Overexpression of c Src did not prevent suppression of STAT 5 activity by E 2 and ERalpha . ^^^ However , ERalpha did prevent basal increases in STAT 5 activity with overexpressed c Src . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
One class of steroid receptors that c Src modulates is the estrogen receptor alpha ( ERalpha ) . ^^^ Although there is strong biochemical evidence supporting a role for c Src in ERalpha signaling , the consequence of this association is unclear at the biological level . ^^^ To explore the significance of c Src in ERalpha signaling , we studied the development of various reproductive organs that are dependent on ERalpha in c Src deficient mice . ^^^ Taken together , these studies demonstrate that c Src plays a role in ERalpha signaling in vivo . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Inhibition of c Src activity blocked proliferation of all tamoxifen resistant variants , suggesting that inhibitors of c Src Fak activity may delay or prevent progression and metastasis of ER positive tumors . ^^^ These studies also raise the possibility that fully active forms of c Src and Fak in breast tumors may be biomarkers to predict tamoxifen resistance and / or risk of recurrence in ER positive breast cancer . . ^^^ Role of c Src and focal adhesion kinase in progression and metastasis of estrogen receptor positive breast cancer . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The juxtanuclear aggregation of pp60c src containing patches depends on microtubules and does not seem to occur within the Golgi apparatus and the rough ER . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Phosphorylation of the human estrogen receptor on tyrosine 537 in vivo and by src family tyrosine kinases in vitro . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Prognostic factors for fibromatoses : a correlation of proliferation index , estrogen receptor , p 53 , retinoblastoma , and src gene products and clinical features with outcome . ^^^ MATERIALS AND METHODS : Proliferation index ( MIB 1 ) , p 53 , src , retinoblastoma gene protein products , estrogen receptor level , site and depth of lesion were correlated with incidence of recurrence in 52 patients . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The existence of an estrogen receptor containing , multimeric complex consisting of hsp 90 , src , and B Raf also suggests a direct link between the estrogen receptor and the MAP kinase signaling cascade . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Together , these data indicate that a Src type tyrosine kinase is necessary for the initiation of Ca ( 2+ ) release from the egg ER at fertilization and identify a Src type p 57 protein as a candidate in the signaling pathway leading to this Ca ( 2+ ) release . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The rapid effect of IFN on ER ligand binding is postulated to reflect phosphorylation of the receptor at Tyr 537 by p56lck , a member of the Src family of PKC activated tyrosine kinases . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Using a mammalian two hybrid assay , we show that the thyroid hormone receptor beta ( TRbeta ) and estrogen receptor beta ( ERbeta ) have different LXXLL motif preferences for interactions with SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
To investigate the molecular mechanisms underlying the ER subtype selective actions of these compounds , we have determined the conformational changes induced in ERalpha and ERbeta by these ligands using protease digestion sensitivity , and we have tested the ability of these ligands to promote the recruitment of representatives of the three SRC / p160 coactivator protein family members ( SRC 1 , GRIP 1 , ACTR , respectively ) to ERalpha and ERbeta using yeast two hybrid and glutathione S transferase ( GST ) pull down assays . ^^^ Conformational changes and coactivator recruitment by novel ligands for estrogen receptor alpha and estrogen receptor beta : correlations with biological character and distinct differences among SRC coactivator family members . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We investigated whether the nonreceptor Src tyrosine kinase could affect ER activity . ^^^ Expression of constitutively active Src or stimulation of the endogenous Src / JNK pathway enhances transcriptional activation by the estrogen ER complex and strongly stimulates the otherwise weak activation by the unliganded ER and the tamoxifen ER complex . ^^^ Src affects ER activation function 1 ( AF 1 ) , and not ER AF 2 , and does so through its tyrosine kinase activity . ^^^ We therefore suggest that the Src / JNK pathway enhances AF 1 activity by modification of ER AF 1 associated proteins . ^^^ Potentiation of estrogen receptor activation function 1 ( AF 1 ) by Src / JNK through a serine 118 independent pathway . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Hormone dependent transcriptional activation of ER and other nuclear receptors involves assembly of a coactivation complex which includes various cofactors such as the steroid receptor coactivators ( SRC ) and CREB binding protein ( CBP ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Structure function evaluation of ER alpha and beta interplay with SRC family coactivators . ^^^ We describe a ligand that binds to both receptors , but enhances only ER beta interaction with SRC 1 and SRC 3 while exhibiting little effect on the ER alpha interaction with these proteins . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In turn , stimulation of Src activity is abolished in ERalpha expressing NIH 3T3 fibroblasts by co transfection of the dominant negative p85alpha and in MCF 7 cells by the PI 3 kinase inhibitor , LY 294002 . ^^^ Hormone stimulation of MCF 7 cells rapidly triggers association of ERalpha with Src and p 85 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We have used an in vitro chromatin assembly and transcription system to examine the biochemistry of interactions among ERalpha , the SRC proteins and p300 / CBP . ^^^ Furthermore , we show that ERalpha SRC p300 / CBP interactions are necessary and sufficient for the targeted acetylation of nucleosomal histones on estrogen responsive promoters in the absence of transcription . ^^^ Finally , we show that the major role of SRC p300 / CBP interactions is to enhance ERalpha mediated transcription initiation , and they have little or no role in stimulating subsequent rounds of transcription . ^^^ Together , our results indicate a specific role for the SRC and p300 / CBP coactivators , as well as targeted histone acetylation , in ERalpha mediated transcription . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Using selective inhibitors , we also demonstrated that ERalpha and Src are upstream regulators of Shc . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
However there is no information on the consequences of reducing SRC 1 , TIF 2 , or SRA expression , singly or in combination , on ERalpha transcriptional activity . ^^^ Furthermore , treatment of cells with combinations of SRA , SRC 1 , and TIF 2 asODNs reduced ERalpha transcriptional activity to an extent greater than individual asODN treatment alone , suggesting that these coactivators cooperate , in at least an additive fashion , to activate ERalpha dependent target gene expression . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Our coactivator interaction assay utilizes time resolved fluorescence technology to assess the binding of the 10 NR boxes derived from the three known p 160 coactivators ( SRC 1 , 2 , 3 ) to the ER subtypes in the presence of each ligand . ^^^ In addition , we examined the preference of E 2 bound ER alpha and ER beta for various naturally occurring NR boxes including the 10 SRC boxes as well as the motifs from PGC 1 , TRBP , TRAP 220 , and CBP . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Specific inhibitors of ER ( Ici 182 , 780 ) , ERK ( U 0125 ) , and Src ( PP 2 ) inhibited in vitro 17 beta estradiol induced cholangiocyte proliferation . ^^^ This might suggest that pharmacologic modulation of ER , ERK , and / or Src could be proposed for the treatment of human pathology characterized by dysregulation of cholangiocyte proliferation . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Here we show that MNAR modulates estrogen receptor ( ER ) interaction with members of the Src family of tyrosine kinases , which leads to a stimulation of Src enzymatic activity and activation of Erk 1 and Erk 2 kinases . ^^^ We also show that MNAR , through activation of the Src / Erk phosphorylation cascade , affects ER transcriptional activity and ultimately ER mediated gene expression . ^^^ Estrogen receptor interacting protein that modulates its nongenomic activity crosstalk with Src / Erk phosphorylation cascade . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In addition , we demonstrate synergism between Mediator and p300 / CBP SRC during ERalpha dependent transcription with chromatin templates , but not with naked DNA . ^^^ Interestingly , we find that Mediator has an additional distinct role during ERalpha dependent transcription not shared by p300 / CBP SRC : namely , to promote preinitiation complex formation for subsequent rounds of transcription reinitiation . ^^^ Collectively , our results indicate an important role for Mediator , as well as its functional interplay with p300 / CBP SRC , in the enhancement of ERalpha dependent transcription with chromatin templates . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
SRC 3 overexpression failed to enhance estrogen dependent transcription of any ER combination in OBs . ^^^ These data suggest that the transactivation capacity of various ER isoforms is both SRC species and cell type dependent . . ^^^ Interestingly , the overexpression of the steroid hormone receptor coactivator 1 ( SRC 1 ) resulted in preferential transcriptional enhancement by ERbeta as well as coexpressed ERalpha and ERbeta , whereas SRC 2 overexpression appeared to preferentially enhance ERalpha transactivation . ^^^ Similar overexpression experiments in COS 7 cells exhibited preferential enhancement of ERalpha function with all SRCs , including SRC 3 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Furthermore , we will describe the existing compounds that are either used in treatment or have a potential as therapeutic agents , such as bisphosphonates , Src inhibitors , and selective estrogen receptor modulators . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The ER corepressor , SMRT , enhanced the repression by 17beta estradiol / ER , but ER coactivators , including SRC family members , did not appreciably impact the ER ligand response . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The activation of ERK1 / 2 through 17beta estradiol triggered simultaneous association of endogenous androgen receptor , estrogen receptor alpha and Src . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Flow cytometry , combined Western blot immunoprecipitation kinase assays , and confocal microscopy revealed that both the nonpairing and pairing mu HC assembled with the Ig alpha beta dimer ; however , in contrast to a pairing mu HC , the nonpairing mu HC was retained in the ER cis Golgi compartment , and neither colocalized with the src kinase lyn nor induced tyrosine phosphorylation of Ig alpha after pervanadate treatment of cells . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Because of the specific effect of Src on the location of KDEL R , we tested whether protein transport between ER and the Golgi apparatus involves Src . ^^^ Transport of Pseudomonas exotoxin , which is transported to the ER by binding to the KDEL R is accelerated by inhibition or genetic ablation of Src . ^^^ Protein transport from ER to the Golgi apparatus however , is unaffected by Src deletion or inhibition . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Here , we examined the effects that different ERalpha ligands have on coactivator protein steady state levels and demonstrate that the selective ER modulators ( SERMs ) 4 hydroxytamoxifen ( 4HT ) and raloxifene are able to elevate SRC 1 and SRC 3 protein levels . ^^^ Selective estrogen receptor modulators 4 hydroxytamoxifen and raloxifene impact the stability and function of SRC 1 and SRC 3 coactivator proteins . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Here we show that the Src homology 2 / 3 ( SH2 / SH3 ) domain containing protein Nck 1 prevents the unfolded protein response normally induced by pharmacological endoplasmic reticulum ( ER ) stress agents . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Expressions of ER , enzyme c PDE and 67kDaLN R in SRC were evidently higher than that in mucinous adenocarcinoma , while expressions of LN , CN 4 , CD44v6 , and PTEN protein were obviously lower in SRC than that in mucinous adenocarcinoma ( P < 0 . 05 ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Rapid extranuclear signaling by the estrogen receptor ( ER ) : MNAR couples ER and Src to the MAP kinase signaling pathway . ^^^ Now , Wong et al . have identified MNAR ( modulator of nongenomic activity of estrogen receptor ) as an adaptor protein that allows the ER to bridge the signaling pathways of tyrosine kinases ( i . e . , Src ) and the mitogen activated protein kinase ( MAPK ) cascade . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We show that in the human breast carcinoma T47D cells , upon estrogen treatment , p130Cas rapidly and transiently associates with the estrogen receptor alpha in a multi molecular complex containing the c Src kinase and the p 85 subunit of PI 3 kinase . ^^^ Association of p130Cas with the estrogen receptor alpha occurs within 3 minutes of estrogen treatment and is dependent on c Src kinase activation . ^^^ Therefore , our data show that the adaptor protein p130Cas associates with the estrogen receptor transducing complex , regulating estrogen dependent activation of c Src kinase and downstream signaling pathways . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Inhibitors were employed to determine pathway ( ER , EGFR , membrane organization , PI 3 kinase , Src kinase , Ca2+ ) involvement and timing of pathway activations ; all affected ERK activation as early as 3 6 min , suggesting simultaneous , not sequential , activation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In starfish eggs , studies using inhibitors designed against vertebrate proteins have shown that this Ca2+ rise requires an egg Src family kinase ( SFK ) that directly or indirectly activates phospholipase C gamma ( PLC gamma ) to produce IP 3 , which triggers Ca2+ release from the egg ' s endoplasmic reticulum ( ER ) [ reviewed in Semin . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Nongenomic effects of 17beta estradiol in human platelets : potentiation of thrombin induced aggregation through estrogen receptor beta and Src kinase . ^^^ These effects were dependent on estrogen receptor beta recruitment and were associated with a strong synergistic effect with thrombin on Src activation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Rb potentiation of SRC coactivators is exerted on a subset ( Nur factors , hepatocyte nuclear factor 4 ( HNF 4 ) , SF 1 , and ER ) but not all NRs . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We isolated 12 transcriptionally active genomic modules that recruit ERalpha and the coactivator steroid receptor coactivator ( SRC ) 3 to different intensities in vivo . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Estrogen triggered activation of Src can be regulated by the modulator of nongenomic actions of the estrogen receptor ( MNAR ) , which binds to estrogen receptors and Src to create a signaling complex . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In these cells , activation of the Src and the PI 3 K dependent pathways is simultaneous and mediated by direct interactions of the two kinases with ERalpha . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The process requires Src and erk signaling and eNOS phosphorylation by phosphoinositide 3 kinase ( PI 3 kinase ) Akt kinase , with Src and PI 3 kinase associating with ERalpha upon ligand activation . ^^^ The loss of NLS2 / NLS3 prevented Src and erk activation , and it altered ligand induced PI 3 kinase ERalpha interaction and prevented eNOS phosphorylation . ^^^ Loss of the DNA binding domain did not change E 2 activation of Src or erk , but ligand induced PI 3 kinase ERalpha binding and eNOS phosphorylation did not occur . ^^^ Thus , dimerization is not required for ERalpha coupling to eNOS ; however , NLS2 / NLS3 plays a role in Src activation , and the DNA binding region is involved in the dynamic interaction between ERalpha and PI 3 kinase . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
A novel connection was identified between src kinase and serine 167 phosphorylation in estrogen receptor ( ER ) alpha via activation of AKT kinase . ^^^ Src effects on ER phosphorylation and SRC 1 activity both contributed to tamoxifen agonist action on ER dependent gene expression in Ishikawa cells . ^^^ Taken together , these data demonstrate that src kinase potentiates tamoxifen agonist action through serine 167 dependent stabilization of ER promoter interaction and through elevation of SRC 1 and cAMP response element binding protein binding protein coactivation of ER . . ^^^ The Src kinase pathway promotes tamoxifen agonist action in Ishikawa endometrial cells through phosphorylation dependent stabilization of estrogen receptor ( alpha ) promoter interaction and elevated steroid receptor coactivator 1 activity . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We identified 18 SRC 3 genomic binding sites and demonstrated estrogen receptor alpha ( ERalpha ) binding to all of them . ^^^ Both E 2 dependent and independent SRC 3 / ERalpha binding sites were identified . ^^^ RNA polymerase 2 ChIP assays were used to determine the correlation between SRC 3 and ERalpha binding and recruitment of the transcriptional machinery . ^^^ These assays , in conjunction with analyses of RNA obtained from E 2 treated cells , lead to the identification of SRC 3 / ERalpha associated genes . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In the presence of estradiol ( E 2 ) , ERalpha , Src homology and collagen homology ( Shc ) , and insulin like growth factor receptor 1 proteins associate with and increase the localization of ERalpha at the membrane . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
OBJECTIVES : Fibroid tumor growth in the myometrium appears to be regulated by estrogens but the role of estrogen receptor ( ER ) coregulators , such as the steroid receptor coactivator ( SRC ) family members , in fibroid growth is currently unknown . ^^^ The aims of this study were to compare the expression of the SRC 1 , SRC 2 , and SRC 3 coactivators between fibroids and normal myometrium in pure populations of cultured smooth muscle cells ( SMC ) and microvascular endothelial cells ( MEC ) , and also between both cell types , and to identify any relationship between the SRC expression profiles and the known ER status of the SMC and MEC samples examined in this study . ^^^ Although the SRC family members are likely to play a role in the response of fibroid SMC to estrogen , via ERalpha , changes in their levels do not appear to contribute to the increased sensitivity of fibroid SMC to estrogen . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In addition , the specific estrogen receptor antagonists ICI 182 , 780 ( faslodex ) and tamoxifen were noted to be able to strongly inhibit diosgenin induced , src kinase dependent Akt and p 38 MAPK activation . ^^^ Taken together , such results provide evidence that diosgenin up regulates VEGF A and promotes angiogenesis in preosteoblast like cells by a hypoxia inducible factor 1alpha dependent mechanism involving the activation of src kinase , p 38 MAPK , and Akt signaling pathways via estrogen receptor . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Estrogen rapid action via protein complex formation involving ERalpha and Src . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In the present study , to assess the impact of these agents on brain sexual differentiation , region specific mRNA expression of estrogen receptors ( ER ) alpha and beta , the progesterone receptor ( PR ) , gonadotrophin releasing hormone , steroid receptor coactivators ( SRC ) 1 and 2 , and calbindin D in microdissected hypothalamic medial preoptic areas ( MPOAs ) at postnatal day 10 was first analyzed in rats exposed to 0 . 5 ppm EE from gestational day 15 by real time RT PCR . ^^^ Sexually dimorphic expression of ER alpha and PR was noted with predominance in females and males , respectively , EE up regulating SRC 1 in males and ER beta and PR in females . ^^^ Next , we similarly examined expression changes of ER alpha and beta , PR , and SRC 1 in animals exposed to MXC at 24 , 240 , and 1200 ppm , DINP at 4000 and 20 , 000 ppm , and GEN at 1000 ppm . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Here , we show that , through EGF receptor ( EGFR ) and erb B 2 , EGF induces tyrosine phosphorylation of ER preassociated with AR , thereby triggering the assembly of ER / AR with Src and EGFR . ^^^ Interestingly , EGF triggers rapid association of Src with androgen receptor ( AR ) and estradiol receptor alpha ( ERalpha ) in MCF 7 cells or ERbeta in LNCaP cells . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
PELP 1 ( proline , glutamic acid , and leucine rich protein 1 ) ( also known as the modulator of nongenomic activity of estrogen receptor ) plays a role in genomic functions of the estrogen receptor via histone interactions and in nongenomic functions via its influence on the MAPK Src pathway . ^^^ HRS was found to sequester PELP 1 in the cytoplasm , leading to the activation of MAPK in a manner that is dependent on the epidermal growth factor receptor but independent of the estrogen receptor , Shc , and Src . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Recently , it has been shown that 17beta estradiol ( E 2 ) induces a rapid and transient activation of the Src ERK phosphorylation cascade : a clear indication that the alpha oestrogen receptor ( ERalpha ) is able to associate with the plasma membrane . ^^^ One of the signal transduction components found to accumulate in caveolae is Src kinase in an amount that increases simultaneously with increases in the amount of ERalpha . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
EXPERIMENTAL DESIGN : Using immunohistochemistry and image analysis , we quantified the levels of phospho Stat 3 ( pY Stat 3 ) , phospho Src ( pY Src ) , epidermal growth factor receptor , HER2 / neu , Ki 67 , estrogen receptor , Bcl 2 , Bcl xL , Survivin , and apoptosis in formalin fixed , paraffin embedded sections from invasive carcinomas and their paired nonneoplastic parenchyma . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Based on the reports demonstrating aPKCiota Src interaction and Src activity in the retrograde pathway ( Golgi ER ) , studies were initiated to learn whether Rab 2 also promoted Src recruitment to VTCs . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Furthermore , Erk1 / 2 activation is abrogated upon treatment with the Src inhibitor PP 2 , suggesting a role played by a Src family member in the pathway from the ER . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Deletion of the ER targeting sequence resulted in cytosolic localization and altered the distribution of PTP1B at cell matrix foci , whereas mutations disrupting interactions with Src homology 3 ( SH 3 ) domains , and the insulin and cadherin receptors had no effect . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We demonstrated that the recruitment of LMP 2 by SRC coactivators is necessary for cyclic association of ER regulated transcription complexes on ER targets . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Coexpression of SRC 1 reversed the ability of the estrogen receptor to squelch activation by hPR . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Coexpression of CBP and SRC 1 stimulated ER and PR transcriptional activity in a synergistic manner and indicated that these two coactivators are not functional homologues . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
To explore a possible role of steroid receptor coactivators in transcriptional synergism between AF 1 and AF 2 , we expressed the amino terminal ( AF 1 containing ) and carboxyl terminal ( AF 2 containing ) regions of ER as separate polypeptides in mammalian cells , along with the steroid receptor coactivator 1 protein ( SRC 1 ) . ^^^ We demonstrate that SRC 1 , which has been shown to significantly increase ER transcriptional activity , enhanced the interaction , mediated by either E 2 or TOT , between the AF 1 containing and AF 2 containing regions of the ER . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Surprisingly , the coactivator SRC 1 up regulates mER beta transactivation both in the absence and presence of E 2 , and in vitro interaction between SRC 1 and the ER beta ligand binding domain is enhanced by E 2 . ^^^ Moreover , the ligand independent stimulatory effect of SRC 1 on ER beta transcriptional activity is abolished by ICI 182 , 780 , but not by OHT . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
To determine whether coactivators and corepressors have the capacity to modulate the relative agonist / antagonist activity of 4HT , ER dependent gene expression was measured in the absence or presence of expression vectors for SRC 1 ( steroid receptor coactivator 1 ) or SMRT ( silencing mediator of retinoic acid and thyroid hormone receptors ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
CBP and SRC 1 interact and synergistically enhance transcriptional activation by the ER and PR . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We have analyzed several transcriptional steps that could be involved in the activation : the ability of these receptors 1 ) to interact with several coactivators ( steroid receptor coactivator 1 , SRC 1 ; transcription intermediary factor 1 , TIF 1 ; and estrogen receptor associated protein 140 , ERAP 140 ) and with members of the preinitiation complex [ TATA box binding protein ( TBP ) , transcription factor IIB ( TFIIB ) ] ; 2 ) to exhibit conformational changes revealed by proteolytic digest patterns similar to those observed for the wild type hormone occupied ER ; and 3 ) to bend estrogen response element containing DNA , which is thought to be one of the important phenomena triggering transcriptional activation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In addition , EM 652 blocks the E 2 dependent activation of ER alpha and ER beta by the steroid hormone receptor coactivator 1 as well as the in vitro interaction between SRC 1 and the ligand binding domains of both ERs . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Steroid receptor co activator ( SRC 1 ) is one of a number of transcriptional co activators that are capable of potentiating the activity of nuclear receptors including the oestrogen receptor ( ER ) . ^^^ Thus , SRC 1 exists as functionally distinct isoforms which are likely to play different roles in ER mediated transcription . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Steroid receptor coactivator 1 ( SRC 1 ) appears to be a general coactivator for steroid receptors and rate limiting factor necessary for efficient ER transactivation . ^^^ There was no relationship between the levels of SRC 1 in these primary tumors and the proportion of tumor cells within the surgical samples , nor with ER status . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The two cofactors for steroid receptors RIP 140 and SRC 1 do not seem to be specifically involved in the insulin induced ER alpha transactivation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Up regulation of the steroid receptor coactivator SRC 1 did not seem to mediate the process of enhanced ER induced transcription . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Steroid receptor coactivator 1 ( SRC 1 ) family members interact with steroid receptors , including estrogen receptor alpha ( ERalpha ) and progesterone receptor ( PR ) , to enhance ligand dependent transcription . ^^^ However , the expression of ERalpha and SRC 1 was found to be segregated in distinct subsets of cells within the epithelium of the estrogen responsive rat mammary gland . ^^^ This finding was in contrast to the finding for the stroma , where significant numbers of cells coexpressed ERalpha and SRC 1 . ^^^ Treatment of animals with estrogen induced PR expression in the ERalpha expressing mammary epithelial cells in the absence of detectable SRC 1 and did not affect the segregated pattern of SRC 1 and ERalpha expression . ^^^ PR was neither expressed nor induced by estrogen treatment in stroma , despite the coexpression of ERalpha and SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We also show that overexpression of the steroid receptor coactivator ( SRC 1 ) potentiates transactivation by genistein activated ER alpha and that coexpression of CBP ( the cAMP response element binding protein coactivator ) synergistically increases this signal . ^^^ In in vitro binding assays , we show that genistein , but not 4 hydroxytamoxifen , induces a direct interaction between radiolabeled ER alpha and a GST SRC 1 fusion protein . ^^^ More importantly , coincubation with genistein and 4 hydroxytamoxifen or genistein treatment following preincubation of the ER with 4 hydroxytamoxifen also resulted in a strong physical interaction with SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We have analyzed the contribution of acidic AF 2 residues to the process of ER coactivation by the steroid receptor coactivator , SRC 1 . ^^^ In HeLa cells , SRC 1 coexpression was found to restore transcriptional potency to otherwise inert complexes of wild type ER and 4 hydroxyestratrien 17beta ol . ^^^ SRC 1 coexpression also enhanced transcriptional activity of reporter genes induced by an ER mutant with neutral replacements to acidic AF 2 residues , in response to E 2 or 4 hydroxyestratrien 17beta ol . ^^^ Changes in the structure of the ligand or substitutions to AF 2 residues in the estrogen receptor make independent contributions to coactivator sensitivity by SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
By two hybrid assay in yeast , the interactions of estrogen receptor with RIP 140 , SRC 1 , TIF 1 , and TIF 2 were detected and they were completely dependent on the presence of estrogen . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In affinity interaction assays for proteins that associate specifically with the hormone binding domain of these receptors , we demonstrate that the steroid receptor coactivator SRC 1 interacts in an estrogen dependent manner with ERalpha and ERbeta 1 , but not with ERbeta 2 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
However , TERP 1 may compete with ER for binding sites of receptor cofactors because steroid receptor coactivator 1 ( SRC 1 ) rescued the inhibitory actions of TERP 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
EM 652 inhibits Ras induced transcriptional activity of ER alpha and ER beta and blocks SRC 1 stimulated activity of the two receptors . ^^^ EM 652 was also found to block the recruitment of SRC 1 at AF 1 of ER beta , this ligand independent activation of AF 1 being closely related to phosphorylation of the steroid receptors by protein kinase . ^^^ Most importantly , the antiestrogen hydroxytamoxifen has no inhibitory effect on the SRC 1 induced ER beta activity while the pure antiestrogen EM 652 completely abolishes this effect , thus strengthening the need to use pure antiestrogens in breast cancer therapy in order to control all known aspects of ER regulated gene expression . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
This binding did not interfere with either the ERalpha dimerization or the interaction between hERalpha and its coactivator SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We used a fluorescent ligand for ER , tetrahydrochrysene ketone , to monitor the rates of ligand dissociation from ERalpha and ERbeta , and to see how this process is affected by the p 160 class coactivator , steroid receptor coactivator 1 ( SRC 1 ) . ^^^ We used a 15 amino acid peptide corresponding to the second nuclear receptor box LXXLL motif in SRC 1 ( NR 2 peptide ) , which is known to interact with the ER ligand binding domain , a mutant peptide with an LXXAL sequence ( NR 2A peptide ) , and a 203 amino acid fragment of SRC 1 , termed the nuclear receptor domain ( SRC 1 NRD ) , embodying all three of the internal NR boxes of this protein . ^^^ Both the NR 2 peptide and the SRC 1 NRD fragment markedly slow the rate of dissociation of the agonist ligands tetrahydrochrysene ketone , estradiol , and diethylstilbestrol , increasing the half life of the ER agonist complex by up to 50 to 60 fold . ^^^ The SRC 1 NRD has much higher potency in retarding ligand dissociation than does the NR 2 peptide ; it is maximally effective at 30 nM , and it appears to bind with the stoichiometry of one SRC 1 NRD per ER dimer . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
ASC 2 also interacted with other nuclear receptors , including retinoic acid receptor , thyroid hormone receptor , estrogen receptor alpha , and glucocorticoid receptor , basal factors TFIIA and TBP , and transcription integrators CBP / p300 and SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
This recruitment is in direct contrast to the recruitment of SRC 1 to the estrogen receptor , which requires interaction with the ligand binding domain . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
To obtain some clue to these roles , we screened the expression levels of ER alpha , ER beta , coactivators ( SRC 1 , TIF 2 , AIB 1 , CBP , and P / CAF ) and corepressors ( N CoR and SMRT ) in 6 normal mammary glands , 6 intraductal carcinomas , 22 invasive ductal carcinomas , and 7 breast cancer cell lines using a multiplex reverse transcription PCR . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Because TERP 1 contains a dimerization domain and part of the coactivator binding pocket , we hypothesized that it modulates ER function by direct interactions with full length ER or the steroid receptor coactivator , SRC 1 . ^^^ TERP 1 also binds SRC 1 , and increasing levels of SRC 1 decrease the TERP 1 ERalpha interactions , in agreement with the rescue of TERP 1 suppressed ERalpha transcriptional activity by SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Here we demonstrate that both peroxisome proliferator activated receptor binding protein ( PBP ) and steroid receptor coactivator 1 ( SRC 1 ) are required for ligand dependent transcription of transiently transfected and chromosomally integrated reporter genes by the estrogen receptor ( ER ) and retinoic acid receptor ( RAR ) . ^^^ These findings suggest that ligand dependent transcriptional activities of the RAR and ER require concurrent or sequential recruitment of SRC 1 and PBP containing coactivator complexes . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Experiments performed with bioluminescent derivatives of ERalpha and steroid receptor coactivator 1 ( SRC 1 ) demonstrated both proteins colocalize to the same NM bound foci in response to E 2 but not the antagonists tested . ^^^ Collectively , our data suggest that ERalpha transcription function is dependent upon dynamic early events including intranuclear rearrangement , NM association , and SRC 1 interactions . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
However , unlike estrogens , none of these xenobiotics seemed to be able to induce ER / SRC 1 interactions , most likely because the conformation of the activated receptor would not allow direct contacts with this coactivator . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Constitutive uterine mRNA expression of switch protein for antagonist ( SPA ) , SRC 1 , GRIP 1 , RAC 3 , RIP 140 , and p 300 mRNAs was observed in control uteri , and treatment with ER ligands did not alter coactivator mRNA levels . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Cotransfection of PTalpha or SRC 1 with increasing amounts of REA , as well as competitive glutathione S transferase pulldown and mammalian two hybrid studies , show that REA competes with PTalpha ( or SRC 1 ) for regulation of ER transcriptional activity and suppresses the ER stimulation by PTalpha or SRC 1 , indicating that REA can function as an anticoactivator in cells . ^^^ Our data support a model in which PTalpha , which does not interact with ER , selectively enhances the transcriptional activity of the ER but not that of other nuclear receptors by recruiting the repressive REA protein away from ER , thereby allowing effective coactivation of ER with SRC 1 or other coregulators . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
REA and the coactivator SRC 1 were involved in a functional competition for regulation of ER transcriptional activity , which we show results from competition between these two coregulators for interaction with ER . ^^^ Rather , this sequence was required for the competitive binding of REA and SRC 1 to ER and thus for optimal repression of ER activity . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The expression of SRC 1 and TIF 2 is significantly related to progesterone but not to estrogen receptor expression . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We demonstrate that transcriptional activation by the estrogen receptor ( ER ) requires functional BRG 1 and that the coactivation of estrogen signaling by either SRC 1 or CBP is BRG 1 dependent . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In addition , the D351G ER retains the ability to bind SRC 1 in the presence of E 2 , thus D351G ER AF 2 activity has not been compromised . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We show that in contrast to SRC 1 , direct binding of CBP to the estrogen receptor is weak , suggesting that SRC 1 functions primarily as an adaptor to recruit CBP and p 300 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Dual FRAP experiments show that fluorescent ER and SRC 1 exhibit similar dynamics only in the presence of E 2 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We previously identified a conserved potential alpha helical structure within the AF 1 functional core , and by evaluating point mutants of human ERalpha ( hERalpha ) within this region , we show that in transfection experiments this structure is required for synergism between SRC 1 and hERalpha . ^^^ This interaction of SRC 1 with the AF 1 alpha helical core is essential for both E 2 and OHT induced ERalpha activity . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Unlike the steroid hormone receptor coactivator 1 ( SRC 1 ) , beta catenin showed a strong interaction with AR but not with other steroid hormone receptors such as estrogen receptor alpha , progesterone receptor beta , and glucocorticoid receptor . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Two nuclear receptor coactivators , steroid receptor coactivator 1 ( SRC 1 ) and cAMP response element binding protein binding protein ( CBP ) , have been shown to act in concert to enhance ER activity in vitro . ^^^ Reduction of SRC 1 and CBP protein in brain disrupted ER mediated activation of the behaviorally relevant progestin receptor gene . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Employment of the human estrogen receptor beta ligand binding domain and co activator SRC 1 nuclear receptor binding domain for the construction of a yeast two hybrid detection system for endocrine disrupters . ^^^ We constructed two hybrid systems that co express the Gal4p DNA binding domain / ligand binding domain of human estrogen receptor ( hER ) alpha or beta and the Gal4p transactivation domain / nuclear receptor binding domain of co activator SRC 1 , TIF 2 , or AIB 1 in Saccharomyces cerevisiae with a chromosome integrated lacZ reporter gene under the control of Gal4p binding sites . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Furthermore , not only AR but also the glucocorticoid receptor YFP , ER alpha GFP , and YFP tagged SRC 1 , TIF 2 , and CBP were found to be accumulated in identical spots in the presence of ligand . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Although PRMT 2 was found to interact with two other coactivators , the steroid receptor coactivator 1 ( SRC 1 ) and the peroxisome proliferator activated receptor interacting protein ( PRIP ) , no synergistic enhancement of ERalpha transcriptional activity was observed when PRMT 2 was coexpressed with either PRIP or SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Collectively , the findings clearly implicate dual regulation of ERalpha dependent function by SRC 1 and SRC 2 in the intact female brain . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Reproductive tracts were collected , weighed , and examined for changes in histomorphology and expression of ER and nuclear receptor co regulators ( SRC 1 , p 300 , CARM 1 , GRIP 1 , SPA , REA and Uba 3 ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In addition to DNA binding , the interaction of both ER subtypes with the Alexa 488 labeled SRC 1 coactivator fragment was investigated by fluorescence anisotropy . ^^^ The interactions of human estrogen receptor subtypes ERalpha and ERbeta with DNA and a 210 amino acid residue fragment of the coactivator protein SRC 1 bearing three nuclear receptor interaction motifs were investigated quantitatively using fluorescence anisotropy in the presence of agonist and antagonist ligands . ^^^ Moreover , estrone and genistein exhibited subtype specificity in that they induced SRC 1 recruitment to ERbeta with much higher efficiency than in the case of ERalpha . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Furthermore , IA R bound ERalpha L384M and wild type ERbeta displayed enhanced interactions with the nuclear receptor interaction domains of the coactivators SRC 1 and GRIP 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Replacement of either TRAP 220 LXXLL motif with the corresponding 13 amino acids of SRC 1 LXM2 strongly enhanced the interaction of the TRAP 220 NID with the estrogen receptor . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
However , mutation of the two cAMP inducible SRC 1 phosphorylation sites important for cAMP activation of chicken progesterone receptor or all seven known SRC 1 phosphorylation sites did not specifically impair cAMP activation of ER alpha . ^^^ Our data suggest that cAMP activation of ER alpha transcriptional activity is associated with receptor instead of SRC 1 phosphorylation . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
To examine the sex steroid dependent growth mechanisms of the human endometrium , the expression of steroid receptor coactivators [ steroid receptor coactivator 1 ( SRC 1 ) and p300 / CREB binding protein ( p300 / CBP ) ] and corepressors ( nuclear receptor corepressor and silencing mediator for retinoid and thyroid hormone receptors ) was examined by immunohistochemistry , using 50 samples of normal endometria , and was compared with that of estrogen receptors ( ER ) , progesterone receptors ( PR ) , and proliferation marker Ki 67 . ^^^ Such change in the expression pattern of SRC 1 resembled that of ER , PR , and Ki 67 . ^^^ Binding of SRC 1 to ER was observed in the proliferative ( but not in the secretory ) endometrium . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
SRC 1 , the p 160 steroid receptor coactivator family member , synergized with XBP 1S or XBP 1U to potentiate ERalpha activity . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In endometrial hyperplasia , there was a significant correlation between the expression of ER and SRC 1 or p300 / CBP . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Reciprocal inhibition between Erg and ERalpha was not alleviated by overexpressing CBP , SRC 1 or RIP 140 , three nuclear coactivator proteins . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
SRC 1 and SMRT were identified at the ER ERE complex , and interactions between ER isoforms and coregulatory proteins were determined using immunoprecipitation . ^^^ Both ER alpha and ER beta preferentially bound SRC 1 in the presence of beta estradiol . ^^^ Differential recruitment of SRC 1 and SMRT by ER alpha and ER beta in the presence of beta estradiol and 4 OHT may be central to the response of the tumor to endocrine treatment . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The tumoral ER alpha protein expression was significantly correlated with that of PgR ( r = 0 . 61 , p = 0 . 001 ) and NCoR ( r = 0 . 4 , p = 0 . 043 ) , whereas ER beta expression was associated with SRC 1 ( r = 0 . 68 , p < or = . 001 ) , TIF 2 ( r = 0 . 64 , p = 0 . 001 ) and NCoR ( r = 0 . 48 , p = 0 . 014 ) protein levels in malignant specimens . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Further , 4EM mediated transcription in ER alpha , like estrogen , was enhanced in the presence of coactivators , steroid receptor coactivator 1 ( SRC 1 ) , CREB binding proteins ( CBP ) , and E 6 associated protein ( E 6 AP ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The mutations impaired the interaction of the ER ligand binding domain with the SRC 1 receptor interacting domain in a mammalian two hybrid system . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Transcriptional activation by estrogen receptor ( ERalpha ) and steroid receptor coactivator ( SRC 1 ) involves distinct mechanisms in yeast and mammalian cells . ^^^ We also show that the ligand dependent activities of ERalpha and progesterone receptor ( PR ) in yeast cells were strongly enhanced by the human p 160 protein steroid receptor coactivator ( SRC 1 ) , but not by CREB Binding Protein ( CBP ) or the p300 / CBP associated factor ( P / CAF ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The selective estrogen receptor modulator tamoxifen acted as a weak agonist of ERalpha mediated gene expression and this weak activity was potentiated by SRC 1 . ^^^ In contrast to previously reported yeast based ERalpha transactivation systems , the system reported here in which SRC 1 functions as a bona fide coactivator should permit a more thorough dissection of the factors involved in ERalpha mediated transcriptional activation . . ^^^ Potentiation of human estrogen receptor alpha mediated gene expression by steroid receptor coactivator 1 ( SRC 1 ) in Saccharomyces cerevisiae . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
METHODS : Gene expression of SRC 1 , SRC 2 , SRC 3 , N CoR , SMRT , ERalpha , and PR was measured in 26 samples of normal endometrium and 30 primary endometrial carcinomas using real time RT PCR . ^^^ In the normal endometrium , SRC 1 mRNA expression was positively correlated with ERalpha protein expression and SRC 3 mRNA expression was positively correlated with patient age . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Inverse relationship between ER beta and SRC 1 predicts outcome in endocrine resistant breast cancer . ^^^ To determine isoform specific expression of ER and coexpression with activator proteins , we examined the expression and localisation of ER alpha , ER beta and the coactivator protein steroid receptor coactivator 1 ( SRC 1 ) by immunohistochemistry and immunofluorescence in a cohort of human breast cancer patients ( n=150 ) . ^^^ Protein expression of ER beta and SRC 1 was inversely associated ( P=0 . 0001 ) . ^^^ The association of ER beta protein expression with increased DFS and its inverse relationship with SRC 1 suggests a role for these proteins in predicting outcome in breast cancer . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
CHO K 1 cells transfected with ERalpha or ERbeta show ERE sequence dependent differences in the functional interaction of ERalpha and ERbeta with coactivators steroid receptor coactivator 1 ( SRC 1 ) , SRC 2 ( glucocorticoid receptor interacting protein 1 ( GRIP 1 ) ) , SRC 3 amplified in breast cancer 1 ( AIB 1 ) and ACTR , cyclic AMP binding protein ( CBP ) , and steroid receptor RNA activator ( SRA ) , corepressors nuclear receptor co repressor ( NCoR ) and silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) , and secondary coactivators coactivator associated arginine methyltransferase 1 ( CARM 1 ) and protein arginine methyltransferase 1 ( PRMT 1 ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Ligand dependent estrogenic responses in NSCLC cells are probably generated via ERbeta and the p 160 coactivator GRIP1 / TIF2 , because expression of these proteins was detected , but not full length ERalpha or the p 160 coactivator SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We additionally analyzed the participation of the coactivators SRC 1 and cAMP response element binding protein ( CREB ) binding protein ( CBP ) in FSH evoked estrogen receptor ( ER ) dependent transactivation ; we found that CBP but not SRC 1 potentiated FSH induced transcriptional activation of both ER sensitive reporters , being this effect stronger on the ERE VitA 2 TK CAT than on the 3X ERE TAT Luc reporter . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
We conclude that ( 1 ) TRbeta 1 , like AR and ER , is subject to acetylation ; ( 2 ) the process of acetylation of TR requires thyroid hormone directed MAPK activity , but not serine phosphorylation of TR by MAPK , suggesting that the contribution of MAPK is upstream in the activation of the acetylase ; ( 3 ) the amino acid residue 128 142 region of the DBD of TR is important to thyroid hormone associated recruitment of p 300 and SRC 1 ; ( 4 ) acetylation of TR DBD mutants that is directed by T 4 appears to be associated with recruitment of p300 . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
The aim of the study was to test the hypothesis that expression of retinoid receptors ( RARalpha , RARbeta , RARgamma ) , rexinoid receptors ( RXRalpha , RXRbeta ) , thyroid hormone receptors ( TRalpha , TRbeta ) , estrogen receptors ( ERalpha , ERbeta ) , nuclear receptor coregulators ( N CoR , SRC 1 , SMRT ) , and in addition type 1 iodothyronine 5 ' deiodinase ( 5 ' DI ) , EGFR and erb B2 / neu would be different in mammary postlactating tissue in comparison with that of nonlactating mammary gland . ^^^ Using RT PCR , we have shown that expression of RARalpha , RXRalpha , TRalpha , ERalpha , ERbeta , N CoR , SRC 1 , SMRT and EGFR in rat was significantly increased in postlactating mammary gland when compared to that of nonlactating mammary tissue . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Using small interfering RNA , we determined that induction is dependent on the presence of PR , estrogen receptor and SRC 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation assays further demonstrate that MUC 1 ( 1 ) associates with ERalpha complexes on estrogen responsive promoters , ( 2 ) enhances ERalpha promoter occupancy , and ( 3 ) increases recruitment of the p 160 coactivators SRC 1 and GRIP 1 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
A combination of the full length human estrogen receptor alpha with the nuclear receptor binding domain of co activator steroid receptor co activator 1 ( SRC 1 ) or transcriptional intermediate factor 2 ( TIF 2 ) was most effective for estrogen dependent induction of the chromosome integrated UAS ( GAL ) CYC 1 ( p ) lacZ reporter construct among the two hybrid systems so far tested . . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
In this work we have determined the role of the 26S proteasome in the regulation of the content of progesterone receptors ( PR A and PR B ) , estrogen receptors ( ER alpha and ER beta ) , the coactivator SRC 1 and the corepressor SMRT in the rat brain during the estrous cycle . ^^^ The 26S proteasome inhibitor MG 132 was injected once into the lateral ventricle on proestrous day ; and 24h later , on estrous day we evaluated the content of PR and ER isoforms , SRC 1 and SMRT in the hypothalamus , the preoptic area and the hippocampus by Western blot . ^^^ A significant increase in the content of both PR isoforms , ER beta and SRC 1 was observed after the administration of MG 132 in the three studied cerebral regions . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Herein , the molecular mechanisms involved in ER subtype selective interactions of these compounds as assessed by their effects upon both ERalpha and ERbeta structural conformation and their ability to induce recruitment of steroid receptor coactivator 1 ( SRC 1 ) to ERalpha were investigated . ^^^ In addition , these compounds had the ability to recruit SRC 1 to the ligand binding domain of ERalpha similar to E ( 2 ) . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation analysis showed that E ( 2 ) induced recruitment of C / EBPbeta , ERalpha , SRC 1 , p 300 , pCAF , TFIIB , and Pol 2 , with no change in Sp1 / Sp3 . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Previously , our lab has shown that SRC 1 and CBP modulate estrogen receptor ( ER ) mediated induction of progestin receptor ( PR ) gene expression in the ventromedial nucleus of the hypothalamus ( VMN ) and hormone dependent sexual receptivity in female rats . ^^^ In the present experiments , the function of SRC 1 and CBP in distinct ER ( Exp . 1 ) and PR ( Exp . 2 ) dependent aspects of female sexual behavior was investigated . ^^^ In Exp . 1 , infusion of antisense oligodeoxynucleotides to SRC 1 and CBP mRNA into the VMN decreased lordosis intensity in rats treated with E alone , suggesting that these coactivators modulate ER mediated female sexual behavior . ^^^ Taken together , these data suggest that SRC 1 and CBP modulate ER and PR action in brain and influence distinct aspects of hormone dependent sexual behaviors . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
Strikingly , the literature shows that in vivo variations at residues in the new site are linked to androgen resistance and leukemia , and our own targeted mutations to this site lower but do not eradicate transcriptional activation by estrogen receptor alpha ( ERalpha ) , with reduced ligand binding affinity and SRC 1 interaction . ^^^
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P03372	Pubmed	SVM Score :0.0
NA