Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.61485238
Abl was regulated efficiently when its SH 3 domain was replaced with a heterologous SH 3 from c Src that binds a different spectrum of proline rich ligands , but not by substitution of a modular WW domain with similar ligand binding specificity . 0.61485238^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.57263872
Consequence to the phosphorylation is a marked increase of the association between c Abl and p 73 via the binding of tyrosine phosphorylated p 73 to the c Abl Src homology 2 ( SH 2 ) domain . 0.57263872^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Nonreceptor PTK encoded by mammalian proto oncogenes ( c src , fyn , c yes , c abl ) and their homologs in Drosophila exhibit patterns of expression and activities that suggest functions in axonal or dendritic outgrowth . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Oncogenes c myc , c raf , c erb A , c ras H , c ski , and c sis were expressed in both proliferating and differentiated cardiac muscle tissue and cells , whereas c myb expression was not observed in either . c src was expressed only in neonatal cardiac muscle tissue and cells . c fms , c abl , and c ras K were expressed in tissue from both neonatal and adult animals but only in purified cells from neonatal animals . c fes / fps was expressed only in neonatal cardiac muscles cells . c fos expression was not observed in cardiac muscle tissue from either neonatal or adult rats , but surprisingly was abundantly expressed in freshly isolated cardiac muscle cells from animals of both ages . ^^^ They also show that the expression of c fos can be activated by the cell isolation procedure . c myc , c ski , c ras H , c ras K , c abl , c raf and c erb A were expressed in both proliferating and terminally differentiated skeletal muscle cells , whereas c myb , c fos , c src and c fms transcripts were observed only in proliferating cells . c fes / fps and c sis were not expressed in dividing or fused skeletal muscle cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Expression of c src and c abl in embryonal carcinoma cells and adult mouse tissues . ^^^ We have studied the expression of c src and c abl proto oncogenes in early mouse development using embryonal carcinoma ( EC ) cells as a model system , and compared this to the expression pattern in adult tissues . ^^^ In all three EC lines tested ( F 9 , PC 13 , and P 19 ) , c src and c abl mRNA can be detected . ^^^ When F 9 and PC 13 are induced to differentiate they form endodermal cells characteristic of the early embryo , and we found no change in c src or c abl expression . ^^^ These results are consistent with the idea that c src and c abl have important functions in the differentiation of the cell types of the later embryo , but not in those of the early embryo . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The abundance of c src transcripts was unchanged throughout carcinogenesis ; c abl and c mos transcripts were not detected in either preneoplastic or neoplastic livers . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Northern blot analyses revealed that c myc , c src , c H ras , c abl , c sis , and p 53 genes were expressed . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Genetic studies on Drosophila Abl and , more recently , on mouse c Abl and c Src indicate that the functions of these non receptor tyrosine kinases may duplicate activities of other molecules within signal transduction pathways . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
ABP1p is a novel protein with a 50 amino acid C terminal domain that is very similar to the SH 3 domain in the non catalytic region of nonreceptor tyrosine kinases ( including those encoded by the proto oncogenes c src and c abl ) , in phospholipase C gamma and in alpha spectrin . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
When L 6 alpha 1 cells from myotubes , the levels of c abl , c myb , and c Ha ras transcripts remain unchanged , the level of c N ras RNA is augmented , the level of c erbB RNA is markedly reduced , and all other c onc transcripts ( c erbA , c sis , c src , c fes , c fms , c fos , c myc , c Ki ras , and the putative tyrosine kinase transcript of the c fgr gene ) become hardly , if at all , detectable . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The protooncogenes c fes , c fos , and c erbB were expressed very weakly and the transcripts of the oncogenes c mos , c sis , c erbA , c src , and c abl were not observed in any of the 12 SCLC cell lines . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Cytodot hybridization was used to quantitate the levels of rel , c rel , c myc , c myb , c abl , c fms , c Ha ras , c Ki ras , c src , c yes , c mos , and c sis mRNA in REV T transformed cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
FI subclonal lines formed tumors in syngeneic mice and contained no detectable poly A messenger RNA for GM CSF or IL 3 , and no elevation in c myc , c abl , c src , or erb B onc gene specific messenger RNA compared to parent factor dependent lines . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Enhanced expression of c myc and decreased expression of c fos protooncogenes in chemically and radiation transformed C3H / 10T1 / 2 Cl 8 mouse embryo cell lines . c abl , c fos , c Ha ras , c myc , and c mos were expressed whereas c sis , c fms , c rel , c src , and c myb expression was not detectable in C3H / 10T1 / 2 Cl 8 ( 10T1 / 2 ) cells and in eight chemically and radiation transformed 10T1 / 2 cell lines . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This location demonstrates that Dsi 1 is different from 16 of the known proto oncogenes ( c abl , c erbA c erbB , c ets 1 , c ets 2 , c fes , c fos , c myb , c myc , c raf , A raf , c Ha ras , c Ki ras , N ras , c sis , and c src ) and 12 cellular regions of tumor associated integrations in retrovirus induced tumors ( c erbB , Fis 1 , int 1 , int 2 , Mis 1 / pvt 1 , Mlvi 1 , Mlvi 2 , c mos , c myb , c myc , Pim 1 , and c Ha ras ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In the present work the activity of the c abl and c src oncogene encoded tyrosine kinase was investigated during phorbol diester ( TPA ) induced differentiation of the K 562 CML cells . ^^^ Sodium butyrate caused a slight decrease in growth rate and of bcr c abl kinase activity during erythroid differentiation whereas no changes in c src or c abl tyrosine kinase activities were seen in DMSO treated control cells . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
RNA was extracted and analyzed for expression of cellular oncogenes coding for nuclear proteins ( c myc , c myb , c fos ) , the beta chain of platelet derived growth factor ( c sis ) , growth factor receptors or related proteins ( c src , c abl , c fes , c erbB ) , or putative intermediate transducers of mitogenic signals ( c Ha ras , c Ki ras , c N ras ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
No significant expression of c src , c ras , c fos , c fgr , c fps , c fes , c fms , c yes , c rel , c abl , c mos , c sis , and c erb B genes was found in the patients . c myc and c myb expression as having pathogenic and clinical significance is discussed . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Comparison of the sequences between the 5 onc and c onc genes reveals : ( 1 ) The c src , c abl , c mos , c fos , c ras , c myb , c myc , and c sis genes contain coding regions that are highly conserved in the respective 5 onc genes with a small number of base changes . ( 2 ) There are more transitions than transversions . ( 3 ) Some of these base changes are silent mutations and others generate amino acid substitutions in the viral proteins . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This assignment presents a demonstration of the conservation and evolution of enzyme and proto oncogene loci linkage since two cellular homologs of viral oncogenes c src and c abl also map to mouse chromosome 2 . ^^^ In humans c src , ADA , and ITPA remain conserved on chromosome 20 , whereas AK 1 and c abl are together on chromosome 9 . ^^^ These observations and concepts are discussed with respect to the role of proto oncogenes in chromosomal evolution and suggest the long arm of chromosome 6 as a fruitful place to look for c src and c abl in the Chinese hamster . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Comparison of the Drosophila and vertebrate sequences identifies amino acids that may be essential for the distinct functions of the c src and the c abl gene products and places the gene duplication event that has generated the two genes prior to the Chordate Arthropod divergence . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Apparent exon / intron boundaries in the homologous kinase coding regions of c abl and c src are at different locations . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We now report that transcripts of c rasK and c myc genes are significantly elevated after partial hepatectomy , whereas transcripts of c abl and c src are essentially unchanged and transcripts of c mos are undetectable in either normal or regenerating rat liver . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In this report , we describe the construction of a mutant Abl , replacing the ATP binding lobe of c Abl with that of c Src . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We demonstrate that the K protein contains a cluster of at least three SH 3 binding sites ( P 1 , PPGRGGRPMPPSRR , amino acids 265 278 ; P 2 , PRRGPPPPPPGRG , 285 297 ; and P 3 , RARNLPLPPPPPPRGG , 303 318 ) and that each one of these sites is capable of selectively engaging c Src and Vav SH 3 domains but not SH 3 domains of Abl , p 85 phosphatidylinositol 3 kinase , Grb 2 , and Csk . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Precision substrate targeting of protein kinases 5 Abl and c Src . ^^^ The active site substrate specificities of 5 Abl and c Src are compared and contrasted . ^^^ However , c Src and 5 Abl are extremely inefficient as catalysts for certain structural arrangements , including secondary alcohols and primary alcohols containing large substituents in close proximity to the hydroxyl moiety . ^^^ Whereas c Src exhibits a modest preference for aromatic versus aliphatic alcohols , 5 Abl does not . ^^^ Most dramatic is the ability of c Src to utilize short chain alcohols as substrates , an activity virtually absent from the catalytic repertoire of 5 Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This inhibitory activity copurifies with the IR on insulin Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR related insulin like growth factor 1 receptor and the oncoprotein 5 abl but is ineffective against c src tyrosine kinase activity . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We found no detectable transcripts of 9 proto oncogenes ( c sis , c met , c src , c lyn , c fgr , c ros , c pim , Blym and N myc ) , but detected transcripts of 12 other proto oncogenes ( int 2 , erbB , c abl , c raf 1 , c fyn , K ras , H ras , c mos , c myc , c myb , c fos , and c jun ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The importance of an intact Src Homology 2 ( SH 2 ) domain for transformation by members of the tyrosine kinase family , including 5 src , c src , c abl , fps and fyn is well documented . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
An increased level of tyrosine kinase activity , not caused by c Src or c Abl , was further detected in the cytoplasmic fraction of ODC transformed cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The phosphotyrosyl phosphatase ( PTPase ) specificity of phosphotyrosyl phosphatase activator ( PTPA ) stimulated protein phosphatase ( PP ) 2A ( D ) ( rabbit muscle ) and a bona fide PTP 1B ( Xenopus laevis oocytes ) were examined in vitro using phosphotyrosine containing peptides , derived from the phosphorylation sites of p34cdc2 , p50 / HS1 protein , Abl , c Src and c Fgr , as well as the intact phosphoprotein p50 / HS1 and the Src related tyrosine kinases , Lyn and c Fgr . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Phosphatase 1 ( PP 1 ) is phosphorylated `` in vitro ' ' by the tyrosine kinases c src , 5 src and 5 abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Nine of these genes ( H ras , c myc , c fos , c jun , junB , N myc , c abl , c yes , and p 53 ) were expressed in epithelial cells , whereas two ( RB 1 and N ras ) were not . expression of four other genes ( c src , K ras , c raf , and c myb ) was observed , but the intensity of these bands was too low for densitometric analysis . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
High selectivity toward a panel of nonreceptor tyrosine kinases ( c Src , 5 Abl ) and serine / threonine kinases ( PKC alpha , PKA ) was observed . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The kinase specificity of roscovitine was investigated with 25 highly purified kinases ( including protein kinase A , G and C isoforms , myosin light chain kinase , casein kinase 2 , insulin receptor tyrosine kinase , c src , 5 abl ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
High selectivity towards a panel of non receptor tyrosine kinases ( c SRC , 5 Abl ) and serine / threonine kinases ( PKC alpha , PKA ) was observed . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
High selectivity toward a panel of nonreceptor tyrosine kinases ( c Src , 5 Abl and serine / threonine kinases ( PKC alpha , CDK 1 ) was observed . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The human c fes protooncogene encodes a protein tyrosine kinase ( c Fes ) distinct from c Src , c Abl and other nonreceptor tyrosine kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
It is not active against kinases from other receptor families , such as epidermal growth factor receptor , fibroblast growth factor receptor 1 , c Met , and Tie 2 , or intracellular kinases such as c Src , c Abl , and protein kinase C alpha . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In vitro , PDK 1 could be tyrosine phosphorylated by both the c Src and Abl tyrosine kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Both p210bcr abl and 5 src are more susceptible to GA induced degradation than are their normal cellular counterparts , c abl and c src . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A tyrosine to phenylalanine mutation ( Y412F ) at the c Abl residue homologous to the c Src catalytic domain autophosphorylation site impaired the activation of wild type c Abl by 90 % but reduced activation of c Abl P131L by only 45 % . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
It is not active against kinases from other receptor families such as EGFR , FGFR 1 , c Met and Tie 2 or intracellular kinases like c Src , c Abl , PKC alpha . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Here we report that cytoplasmic tyrosine kinase c Abl is an important effector of c Src for PDGF and serum induced DNA synthesis . ^^^ Conversely , the inhibitory effect of Abl PP K ( ) was not compensated by Src . c Src induced c Abl activation involves phosphorylation of Y 245 and Y 412 , two residues required for c Abl mitogenic function . ^^^ These data identify a novel function of cytoplasmic c Abl in the signalling pathways regulating growth factor induced c myc expression and we propose the existence of a tyrosine kinase signalling cascade ( PDGFR / c Src / c Abl ) important for mitogenesis . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This function of c Abl is dependent on kinase activity and is not shared by c Src tyrosine kinase . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The myristoylated N terminal latching to the C terminal lobe of c Abl was recently demonstrated to be an important regulatory element for the kinase , playing a role similar to that of the tyrosine phosphorylated C terminal tail of c Src . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
D QSAR studies on c Src kinase inhibitors and docking analyses of a potent dual kinase inhibitor of c Src and c Abl kinases . ^^^ The ATP binding pocket of the active form of c Src is similar to that of the c Abl kinase in which the activation loop resembles that of an active form . ^^^ One of the potent c Src and c Abl dual kinase inhibitors ( 77 or SKI 606 ) was docked inside the active sites of both c Src and c Abl . ^^^ The results of 3D QSAR analyses and structure based studies will be useful for the design of novel c Src and c Abl dual kinase inhibitors . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NMR binding studies support the hypothesis that an N terminal myristate could bind in this pocket , as observed for Abl , suggesting that c Src may also be regulated by myristate binding . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
High yield bacterial expression of active c Abl and c Src tyrosine kinases . ^^^ We have developed a bacterial expression system for the kinase domains of c Abl and c Src , which allows for the quick expression and purification of active wild type and mutant kinase domains by coexpression with the YopH tyrosine phosphatase . ^^^ This method makes practical the use of isotopic labeling of c Abl and c Src for NMR studies , and is also applicable for constructs containing the SH 2 and SH 3 domains of the kinases . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In this study , the c Abl , c Src , and c Met kinases are produced in high yields in Escherichia coli by using a bicistronic vector encoding the PTP1B tyrosine phosphatase . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A combination of docking / dynamics simulations and pharmacophoric modeling to discover new dual c Src / Abl kinase inhibitors . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
These results suggest that the common TSSA in the mouse and rat cells transformed by RSV or B 77 ASV containing src gene is not shared with mammalian cells infected with retroviruses transducing other oncogenes of the src gene family ( i . e . , fps , fes , and abl ) . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We thus hypothesized that cAbl is important for ROS , cSrc , and Cav 1 dependent growth related AT1R signal transduction . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Secondary structure of Src homology 2 domain of c Abl by heteronuclear NMR spectroscopy in solution . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The expression of the PTK encoding oncogenes fes , abl and src , was investigated with the use of antibodies generated to their respective gene products . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Steady state levels of K ras , H ras , N ras , abl , sis , src , and fos were unchanged in the 10 ray transformed cells compared with non transformed C3H 10T1 / 2 cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We have constructed a series of point mutations in the highly conserved FLVRES motif of the src homology 2 ( SH 2 ) domain of the abl tyrosine kinase . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Activated p60c src also induced antigen independent IL 2 production , whereas two other tyrosine kinases , 5 abl and the platelet derived growth factor receptor , did not . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A detailed analysis of the NIH 3T3 transformants generated from REC : myc : gamma 33 and gamma 41 DNA failed to detect Ha ras , Ki ras , raf , neu , trk , abl , fms , or src oncogenes of rat origin . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Three dimensional solution structure of the src homology 2 domain of c abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
N terminal myristoylation can promote the association of proteins with the plasma membrane , a property that is required for oncogenic variants of Src and Abl to transform fibroblastic cell types . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Compared with bovine cerebrum ( control ) , the oncogenes abl , erbA , fms , fos , K ras , mil , mos , myb , rel , sis , src and yes were expressed equally in both bovine cerebrum and the gliomas . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The steady state mRNA levels of fos , K ras , abl , sis , and src oncogenes were similar in the C3H 10T1 / 2 and TU 2 cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The noncatalytic src homology region 2 segment of abl tyrosine kinase binds to tyrosine phosphorylated cellular proteins with high affinity . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Both the normal human epidermal keratinocytes and cancer cell lines expressed multiple proto oncogenes such as src , erb B 1 , abl , fos , raf , H ras , and myc , and the amount of expression of these oncogenes was notably higher in the cancer cell lines than in the normal keratinocytes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The carcinoma cell lines equally expressed cellular hst , src , abl , and raf proto oncogenes that were not expressed in the normal hamster pouch epithelial cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This domain is shared by a number of signal transduction proteins including nonreceptor tyrosine kinases such as Abl , Fps , Src , and Src family members , the transforming protein Crk , phospholipase C gamma 1 , PI 3 ( phosphatidylinositol ) kinase , and guanosine triphosphatase activating protein ( GAP ) . ^^^ Like the SH 2 domain found in Src , Crk , and Abl , the SH 2 domain of tensin bound specifically to a number of phosphotyrosine containing proteins from 5 src transformed cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Activation of ABL can occur by substitution of the ABL first exon with breakpoint cluster region ( BCR ) sequences or by deletion of the noncatalytic SH 3 ( src homology region 3 ) domain . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
While src and abl tyrosine kinase oncogene products have previously been shown to be inactive in this assay , p65tpr met efficiently induced maturation promoting factor ( MPF ) activation and germinal vesicle breakdown ( GVBD ) together with the associated increase in ribosomal S 6 subunit phosphorylation . tpr met mediated MPF activation and GVBD was dependent on the endogenous c mosxe , while the increase in S 6 protein phosphorylation was not significantly affected by the loss of mos function . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Expression of protooncogenes c myc , N myc , c fos , Ha ras 1 , Ki ras 2 , yes , abl , src , N ras , met and mos was studied in human gastric tumors and in rat gastric mucosal membrane during gastric carcinogenesis induced in rats by means of N methyl N ' nitro N nitroso guanidine ( MNNG ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Efficient fibroblast transformation by proteins of the abl and src oncogene families requires activation of their protein tyrosine kinase activity and membrane association via an amino terminal myristoylation . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We also measured the steady state level of second messengers in 32D cells transformed by erbB , abl , and src oncogenes which abrogate growth factor requirement . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Ras was complexed to 22 % GTP in NIH3T3 cells transformed by 5 src or 5 abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This property was exploited to show that bcr / abl and 5 src but not myc and ras can replace the transforming signal of 5 abl , a result suggesting that the former but not the latter oncogenes transform lymphoid cells via a similar pathway . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Transcripts of mos , erb B , Ki ras , abl and src were not detected . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The analysis includes the oncogenes myc , sis , mos , erbB , erbA , Ha ras , Ki ras , src , fps , and abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In fibroblasts transformed by retroviral oncogenes ( 5 src , 5 abl , 5 fps or 5 fes ) proteins other than the corresponding oncogene coded kinase , were found . ^^^ A p 130 and a p 36 were found in cells transformed by 5 src and 5 abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
RNA isolated from involved skin of psoriatic patients was subjected to dot blot hybridization with cloned viral Ki ras , myc , abl , fos , src , erbB , mos , sis , fes , and yes oncogenes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
It was found that the antibiotic was effective against the cells transformed by tyrosine kinase oncogenes src , yes , fps , ros , abl , erbB , but did not reverse the transformed morphologies induced by oncogenes raf , ras , and myc . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The study is concerned with the expression of src , sis , fos , myc , Ha ras , Ki ras , N ras , mos , and abl proto oncogenes in the brain , heart , liver , kidneys , lung , stomach , cross striated muscle cells and in leukocytes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The c abl proto oncogene encodes a cytoplasmic tyrosine kinase which is homologous to the src gene product in its kinase domain and in the upstream kinase regulatory domains SH 2 ( src homology region 2 ) and SH 3 ( src homology region 3 ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Gene expression analysis showed measurable levels of mRNA for c Ki ras , erb B , erb B 2 , hck , src , lyn , vav , trk , raf 1 , l myc , c jun , c yes , c myc , c abl , and p 53 . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We found that src was unable to transform these variant cells , whether the oncogene was introduced by infection with a murine retrovirus vector or by transfection with plasmid DNA . 3T3 TNR 9 cells were not inherently resistant to transformation , since infection with similar recombinant retroviruses containing either 5 ras or 5 abl did induce transformation . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Growth factor independent 32D src and 32D abl cell lines , established by infecting the interleukin 3 dependent myeloid precursor cell line ( 32D 123 ) with retroviruses containing the src or abl oncogene , were used to study transcriptional regulation of transforming growth factor beta 1 ( TGF beta 1 ) mRNA . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The transcription patterns of Drosophila melanogaster src , abl and two ras homologs were analyzed in normal Drosophila tissue , in neuroblasts derived from tumorous larval brain of the mutant lethal ( 2 ) giant larvae [ l ( 2 ) gl ] and in Schneider 2 tissue culture cells . ^^^ Our results show that , in addition to constitutive transcripts , the src , abl , ras 1 and ras 3 genes express a set of maternal / embryonic specific transcripts . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Extensive analysis of a pool of 15 cellular protooncogenes ( Ha ras , Ki ras , N ras , mos , fos , fes , myc , abl , sis , myb , erbA , erbB , src , raf , N myc ) , using Southern and northern blot analysis , was done to determine whether mutagen induced rearrangement , amplification or overexpression of any of these genes was responsible for the mutagen induced , anchorage independent phenotype . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Lymphoid and mesenchymal tumors in transgenic mice expressing the 5 fps protein tyrosine kinase . src , abl , and fps / fes are prototypes for a family of genes encoding nonreceptor protein tyrosine kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Retroviral expression vectors carrying the tyrosine kinase oncogenes abl , fms , src , and trk abrogate the requirements of murine myeloid FDC P 1 cells for interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
PI turnover is proposed to be regulated by the oncogene protein kinases ( src , ros , abl , fps ) either directly ( acting as PI kinases ) or indirectly ( as tyrosine kinases ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We measured the synthesis of diacylglycerol de novo in normal NIH / 3T3 fibroblasts and in cells transformed by ras , src , sis and abl oncogenes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Analysis of cellular oncogenes showed that myc and fps were amplified approximately tenfold and threefold , respectively , in this cell line , whereas N myc , L myc , N ras , K ras , H ras , abl , erbB 2 , Blym , src , raf 1 , myb , and sis were not changed significantly . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A putative intracellular domain 435 amino acids long shows high homology with the SRC family of tyrosine kinases and within the kinase domain is most homologous with the human insulin receptor ( 44 % ) and 5 abl ( 41 % ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Two regions of phospholipase C ( amino acid residues 555 598 and 668 705 ) exhibited significant amino acid similarities to the products of various tyrosine kinase related oncogenes ( yes , src , fgr , abl , fps , fes , and tck ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The expression of yes , abl , ros , src , erb B , erb A , fms , Ki ras , myb , rel and bas genes was not detected in both cell lines . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The expression of c Ha ras 1 , Ki ras , N ras , abl , src , fos and myc protooncogenes was analyzed in 13 cases of human gastric carcinomas . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The putative onc sequences of S 13 do not hybridize to DNA probes representing src , erb A , erb B , myc , myb , fps , fms , H ras , B lym , abl , rel , and ets . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Taken together , these results suggest that lymphoid transformation in vitro is a specific property of abl and not of src or fps . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The sequence of the erbB gene is furthermore related to that of other viral transforming genes such as src , fps , yes or abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Murine hematopoietic cells with pre B or pre B / myeloid characteristics are generated by in vitro transformation with retroviruses containing fes , ras , abl , and src oncogenes . ^^^ In vitro infection of bone marrow or fetal liver cells with retroviruses containing fes , abl , ras , or src oncogenes resulted in the transformation of early B lineage cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Sis and N ras were moderately hyperexpressed , raf , Blym , and erbA were slightly hyperexpressed , and abl , erbB , fes / fps , fgr , fos , mos , myb , myc , N myc , rasHarvey , rasKirsten , ros , src , and yes were not hyperexpressed in the tumor compared to the control tissue . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Erythroproliferation in vitro can be induced by abl , fes , src , ras , bas , raf , raf / myc , erb B and cbl oncogenes but not by myc , myb and fos . ^^^ The effective transforming viruses were : Friend virus and viruses containing the abl , fes , src , Ha ras , Ki ras , bas , raf , raf / myc , erb B and cbl oncogenes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In addition , abl , fms , fos , myb , src , and yes are transcribed in some or all of the cell lines but not in the normal B cell blasts . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We have found that a high percentage of squamous cell carcinomas have a trisomy in chromosome 2 that carries both src and abl genes and an increased expression of src and abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In contrast , transcripts for abl , src and ros were not detected . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In order to understand the role which known proto oncogenes might play in this process , we have analysed the expression of src , erb A , erb B , ras , abl , sis and mil related genes from Xiphophorus during embryogenesis , in non tumorous organs and in melanoma cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Association with expression of abl , ras , fes , src , erbB , and Cas NS 1 oncogenes but not with myc . ^^^ This antigen was expressed on many pre B and B cell lymphomas , but not on A MuLV transformed fibroblasts , T cell lymphomas , or myelomonocytic leukemias , gp 160 ( 6C3 ) was expressed by most early B lineage spontaneous tumors , and early B tumors induced by replication defective MuLV containing oncogenes the products of which are associated with the cytoplasmic aspect of the plasma membrane , i . e . , fes , abl , H ras , bas , src , erbB , and Cas NS 1 . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We have isolated , in yeast , a nuclear gene named KIN 28 which presents significant sequence homology with the cell division cycle CDC 28 gene , with members of the protein tyrosine kinase family ( src , erb , abl , epidermal growth factor , etc . ) and those of the family of protein kinases phosphorylating serine and threonine . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In this report , we have assigned experimentally a subset of proto oncogenes ( SRC , ABL , FES , and FMS all related to the SRC family ) to Chinese hamster chromosomes by Southern filter hybridization analyses of DNAs isolated from both somatic cell hybrids and flow sorted hamster chromosomes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Expression of the src and abl cellular oncogenes during development of Drosophila melanogaster . ^^^ Transcription of the Drosophila homologs of the abl and src cellular oncogenes , termed Dash and Dsrc , respectively , was studied . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We have isolated phage clones containing the D . melanogaster sequence homologous to the 5 abl oncogene , and two types of phage clones containing sequences homologous to the 5 src probe . ^^^ The D . melanogaster abl clone ( lambda Dabl 1 ) and one of the src clones ( lambda Dsrc 1 ) hybridize with both 5 abl and 5 src probes , and both map in situ to the same chromosomal position , 73B , on chromosome arm 3L . ^^^ The second D . melanogaster src clone ( lambda Dsrc 2 ) does not react with the 5 abl probe and hybridizes in situ to chromosomal position 64B . ^^^ The homologous sequences in D . melanogaster exhibit hybridization to regions in the vertebrate 5 abl and 5 src that are important for kinase activity and transforming potential of the viral gene products . . ^^^ Drosophila melanogaster DNA clones homologous to vertebrate oncogenes : evidence for a common ancestor to the src and abl cellular genes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The amino acid sequence of the putative abl gene , when compared with the sequences of other tyrosine specific protein kinases ( src , fes , fps , and yes ) , revealed significant homologies , indicating that all these functionally related transforming genes are derived from divergent members of the same protooncogene family . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Two dimensional tryptic peptide mapping revealed that p 56 is distinct from the proteins encoded by the cellular genes which are the progenitors of retroviral tyrosine protein kinases , src , yes , fgr , abl , fes , and ros . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We recently developed a technique using a degenerate phosphopeptide library to predict the specificity of individual SH 2 domains ( src family members , Abl , Nck , Sem 5 , phospholipase C gamma , p 85 subunit of phosphatidylinositol 3 kinase , and SHPTP 2 ( Z . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Src homology 2 domain as a specificity determinant in the c Abl mediated tyrosine phosphorylation of the RNA polymerase 2 carboxyl terminal repeated domain . ^^^ Many nonreceptor tyrosine kinases e . g . , Abl and Src also contain SH 2 domains . ^^^ We have previously shown that the carboxyl terminal repeated domain ( CTD ) of the mammalian RNA polymerase 2 is a substrate for the Abl but not the Src tyrosine kinase . ^^^ Mutation of the Abl SH 2 or exchanging it with that of Src , which does not bind the Tyr ( P ) CTD , abolished processivity and reduced the CTD kinase activity without any effect on autophosphorylation or the phosphorylation of nonspecific substrates . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We report that the ena protein contains proline rich motifs and binds to Abl and Src SH 3 domains , ena is also a substrate for the Abl kinase ; tyrosine phosphorylation of ena is increased when it is coexpressed in cells with human or Drosophila Abl and endogenous ena tyrosine phosphorylation is reduced in Abl mutant animals . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
One clone that binds to both Src and Abl SH 3 domains through a common site exhibits reversed binding orientation , in that an arginine indispensable for binding to all tested SH 3 domains occurs at the C terminus . ^^^ Another clone contains overlapping yet distinct Src and Abl SH 3 binding sites . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We have introduced two loss of function point mutations from highly conserved regions of the src homology 3 ( SH 3 ) domains of the Caenorhabditis elegans sem 5 gene into the SH 3 domain of the murine type 4 c abl tyrosine kinase proto oncogene . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Comparison of fyn , src , yes , and abl nonreceptor tyrosine kinase mutant mice shows a specific requirement for Fyn in the induction of long term potentiation at CA 1 synapses in the hippocampus . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In the current study , a tyrosine phosphorylated protein with a molecular mass of approximately 120 kDa was identified which binds only to the Crkl Src homology 2 ( SH 2 ) domain in cells , including Ph positive patient material , containing an active Bcr / Abl protein . ^^^ The Src homology 3 ( SH 3 ) domains of Crkl do not bind to Cbl , but do bind Bcr / Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We examined the importance of the arginine binding site of SH 3 domains by comparing the binding properties of wild type Src SH 3 and Abl SH 3 with those of a Src SH 3 mutant containing a mutated arginine binding site ( D99N ) and Abl SH 3 mutant constructs engineered to contain an arginine binding site ( T98D and T98D / F91Y ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A protein has been identified that interacts specifically with both the Src homologous 3 ( SH 3 ) domain and carboxy terminal sequences of the c Abl tyrosine kinase . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The protein exhibits sequence similarity to homeotic genes , contains several polyproline stretches , and includes a src homology 3 ( SH 3 ) domain at its very carboxyl terminus that is required for binding to Abl proteins . ^^^ When overexpressed in NIH 3T3 cells , abi 1 potently suppresses the transforming activity of Abelson leukemia virus expressing the full length p160v abl kinase but does not affect the transforming activity of viruses expressing a truncated p90v abl or 5 src kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Direct analysis of the binding of the abl Src homology 2 domain to the activated epidermal growth factor receptor . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Moreover , amino acid substitutions around phosphotyrosine in the peptides src ( 412 418 ) , src ( 414 418 ) and abl ( 390 397 ) deeply influence the dephosphorylation efficiency . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Recent crystallographically derived structures of the Src SH 2 domain in complex with low affinity peptides , which do not contain the EEI consensus , and NMR derived structures of unliganded Abl ( ref . 19 ) and p 85 ( ref . 20 ) SH 2 domains have revealed the conserved fold of the SH 2 domain and the properties of a phosphotyrosine binding pocket . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
En bloc substitution of the Src homology region 2 domain activates the transforming potential of the c Abl protein tyrosine kinase . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This is the case whether the catalytic domain of the kinase is derived from Abl or from its distant relative , Src . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NIH3T3 transformants from a tertiary round of transfection were analysed by Southern blot analysis for the presence of Ki ras , N ras , raf , trk , abl , fms , src , mos , fos , sis , fps , erbA , erbB or neu oncogenes of REC origin , and none were detected . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Peptides that contain the RPLPPLP motif compete Src , but not Abl or phospholipase C gamma , SH 3 interactions with SH 3 binding proteins from cell lysates ( IC 50 = 1 5 microM ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Identification of Src , Fyn , Lyn , PI3K and Abl SH 3 domain ligands using phage display libraries . ^^^ We have employed a novel modification of phage display involving biased libraries to identify peptide ligands of the Src , Fyn , Lyn , PI3K and Abl SH 3 domains . ^^^ The Src SH 3 domain prefers the sequence XXXRPLPPLPXP , Fyn prefers XXXRPLPP ( I / L ) PXX , Lyn prefers RXXRPLPPLPXP , PI3K prefers RXXRPLPPLPP while the Abl SH 3 domain selects phage containing the sequence PPPYPPPP ( I / V ) PXX . ^^^ We have also analysed the binding properties of Abl and Src SH 3 ligands . ^^^ We find that although the phage displayed Abl and Src SH 3 ligands are proline rich , they are distinct . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Sequence specificity in the recognition of the epidermal growth factor receptor by the abl Src homology 2 domain . ^^^ The transforming activity of the abl gene product requires a functional src homology 2 ( SH 2 ) domain . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We demonstrate that SH 2 domains from other proteins ( Ras GTPase activating protein , Src , p 85 phosphatidylinositol 3 kinase subunit , and Crk ) can complement the absence of the Abl SH 2 domain and that mutants with heterologous SH 2 domains induce altered patterns of tyrosine phosphorylated proteins in vivo . ^^^ Our data also suggest that the roles of the SH 2 and SH 3 domains in the regulation of Abl are different in several respects from the roles proposed for these domains in the closely related Src family of tyrosine kinases . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The protein binding activity of c Crk is therefore likely regulated by a mechanism similar to that of the Src family kinases . 5 Crk is truncated before c Crk Y 221 and forms constitutive complexes with c Abl and other proteins . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In this study , YMXM containing peptides are shown to act as substrates for two members of the nonreceptor subfamily of tyrosine kinases , 5 Src and 5 Abl ( the transforming gene products of Rous sarcoma virus and Abelson murine leukemia virus , respectively ) . ^^^ The synthetic YMXM containing peptides had among the highest apparent affinities described to date for either tyrosine kinase , with Km values as low as 97 microM for 5 Src and 5 Abl . ^^^ In particular , 5 Src was more tolerant of substitutions at the Met+1 and Met+3 positions in the YMXM motif than either 5 Abl or the insulin receptor kinase but was more dependent on the presence of a preceding acidic amino acid . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Mice with mutations in four nonreceptor tyrosine kinase genes , fyn , src , yes , and abl , were used to study the role of these kinases in long term potentiation ( LTP ) and in the relation of LTP to spatial learning and memory . ^^^ Mutations in src , yes , and abl did not interfere with either the induction or the maintenance of LTP . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
These findings define a new mechanism of oncogenic activation for the ABL kinase through mutations in the last exon which do not require amino terminal deletions or mutations within the src homology regions . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The Tsk cDNA clone encodes a polypeptide of 70 kDa , which is similar in sequence to both the src and abl families of tyrosine kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Using the trk , abl and src families of tyrosine kinase encoded proto oncogenes , we discuss here new insights into the structural and functional organization of neural tissues gained from the molecular and genetic analyses of these genes and their products . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The two glioblastomas with multiple chromosomal changes showed increased EGFr , Ki ras , myb , mos and myc , decreased H ras and N ras and unchanged levels of abl , src and sis . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The growth rates of src , abl or ras oncogene transformed cells , activated raf proto oncogene transformed cells , and normal NIH 3T3 cells were 60 90 % , 20 30 % and 10 % in a serum free medium , respectively , compared to the growth rates in a serum containing medium . ^^^ Herbimycin A , a specific cytoplasmic protein tyrosine kinase inhibitor , selectively inhibited the growth of src or abl transformed cells in the serum free medium resulting in about 10 fold or fivefold lower IC 50 than those in the serum containing medium . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Phenylalanine 420 is perfectly conserved among tyrosine kinases with N terminal SH 3 domains ( the Src and Abl families ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Western blot experiments showed that this protein does not belong to the src family and is not related to the abl and fes oncogene products . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Using a series of conditional chimeras between Abl and Src , we have now further dissected the Abl protein to determine which domains are required for this function . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
RPDE 6 exhibited selectivity in binding to the SH 3 domains of c Abl , Crk , Csk , Lck , Lyn , Fyn and 5 Src , with binding to the SH 3 regions of the Src related tyrosyl kinases Lyn and Fyn being the most effective . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
For the catalytic domain of 5 Src , but not for 5 Abl , addition of exogenous Src SH 3 SH2 domains stimulated the autophosphorylation activity . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The N terminal regulatory region of the Abl protein contains Src homology domains SH 2 and SH 3 which have been shown to be important for the regulation of its activity in vivo . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The c ABL tyrosine kinase is activated following either the loss or mutation of its Src homology domain 3 ( SH 3 ) , resulting in both increased autophosphorylation and phosphorylation of cellular substrates and cellular transformation . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Tyrosine phosphorylated mDab 1 associates with the SH 2 domains of Src , Fyn and Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
STAT 3 expression in particular has been associated with Abl , Src , and HTLV 1 transformation of normal cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
As the three dimensional structures of the phosphatidylinositol ( PI ) 3 kinase , Lck , Src and Abl SH 2 domains have been shown to be similar , we have modelled other SH 2 domains that show distinct sequence specificity to allow comparative analysis of SH 2 phosphopeptide interactions . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
BACKGROUND : The Src homology domains , SH 3 and SH 2 , of Abl protein tyrosine kinase regulate enzymatic activity in vivo . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Distinct ligand preferences of Src homology 3 domains from Src , Yes , Abl , Cortactin , p53bp2 , PLCgamma , Crk , and Grb 2 . ^^^ Specifically , we have found that the Src SH 3 domain selects peptides sharing the consensus motif LXXRPLPXpsiP , whereas Yes SH 3 selects psiXXRPLPXLP , Abl SH 3 selects PPXthetaXPPPpsiP , Cortactin SH 3 selects +PPpsiPXKPXWL , p53bp2 SH 3 selects RPXpsiPpsiR+SXP , PLCgamma SH 3 selects PPVPPRPXXTL , Crk N terminal SH 3 selects psiPpsiLPpsiK , and Grb 2 N terminal SH 3 selects +thetaDXPLPXLP ( where psi , theta , and + represent aliphatic , aromatic , and basic residues , respectively ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Tyrosine phosphorylation at the membrane microfilament interface : a p185neu associated signal transduction particle containing Src , Abl and phosphorylated p 58 , a membrane and microfilament associated retroviral gag like protein . ^^^ Immunoblotting identified two peripheral membrane tyrosine kinases , p6O ( src ) and p 120 ( abl ) , stably associated with the p 185 ( neu ) containing signal transduction particle . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Sixteen amino acid long peptides , corresponding to the optimal ligand preferences of the Src homology 3 ( SH 3 ) domains of Abl , Cortactin , Crk , p53BP2 , and Src , were fused to the N terminus of Escherichia coli alkaline phosphatase ( AP ) . ^^^ In addition , the approximately 60 amino acid long SH 3 domains of Src and Abl were fused to AP and the resulting fusion proteins were found to bind specifically to their respective peptide ligands in microtiter plates and proteins containing proline rich regions in screens of a lambda cDNA expression library . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
From its structure , Cas is deduced to be an ideal substrate for Src family kinases and Abl kinase . ^^^ The associated kinase activity was slightly increased in Src / cells whereas not significantly changed in Abl / nor Fak / cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
These can be divided into nine families : Abl , Fes / Fer , Syk / Zap70 , Jak , Tec , Fak , Ack , Src , and Csk . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
SH 2 domains from a number of signaling proteins , including Lck , Src , and Abl , inhibited apoptosis when present at concentrations of 10 100 nM . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The current information and the data presented here suggest that , although cbl lacks src homology domains , it represents a novel intermediate protein which , by interaction with key SH containing adaptor proteins , may participate in regulation of the Ras and PI 3 kinase pathways in BCR / abl transformed hematopoietic cells . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Consistent with this observation , pp 55 selectively binds to isolated SH 2 domains of Lck , Lyn , Src , and Fyn but not to the SH 2 domains of ZAP 70 , Syk , Shc , SLP 76 , Grb 2 , phosphatidylinositol 3 kinase , and c abl in vitro . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
ArgBP 2 , a multiple Src homology 3 domain containing , Arg / Abl interacting protein , is phosphorylated in 5 Abl transformed cells and localized in stress fibers and cardiocyte Z disks . ^^^ ArgBP 2 contains three COOH terminal Src homology 3 domains , a serine / threonine rich domain , and several potential Abl phosphorylation sites . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Biochemical and genetic evidence suggests that the tyrosine kinase activity of c Abl is tightly regulated in vivo by a cellular factor binding to the Src homology 3 ( SH 3 ) domain of Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The presence of a type 1 ' turn in the BG loop , which is dependent on the presence of a glycine residue at position BG 3 , is indicative of a binding pocket , characteristic of the Src family , SykC and Abl , rather than a binding groove found in PLC gamma 1C , p 85 alpha N and Shc , for example . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Finally , based on sequence alignments and conservation of hydrophobic residues in the Src SH 2 kinase linker as well as in the alphaC beta 4 and beta 2 beta3 loops , we propose that the Src related kinases , Abl , Btk and Csk , share the same quaternary structure . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The Src family kinase Hck interacts with Bcr Abl by a kinase independent mechanism and phosphorylates the Grb 2 binding site of Bcr . bcr abl , the oncogene causing chronic myeloid leukemia , encodes a fusion protein with constitutively active tyrosine kinase and transforming capacity in hematopoietic cells . ^^^ Various intracellular signaling intermediates become activated and / or associate by / with Bcr Abl , including the Src family kinase Hck . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Oncogenic Abl and Src tyrosine kinases elicit the ubiquitin dependent degradation of target proteins through a Ras independent pathway . ^^^ Oncogenic forms of the Abl and Src tyrosine kinases trigger the destruction of the Abi proteins , a family of Abl interacting proteins that antagonize the oncogenic potential of Abl after overexpression in fibroblasts . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The kinases Abl , Btk and Csk seem to have the same molecular architecture as Src . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Thus , we describe some of the relevant interactions between the focal adhesion and the Src and BCR / ABL oncoproteins , respectively . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The isolated catalytic domain of the non receptor tyrosine kinase Abl does not show a preference for the pYEEI motif containing peptide ; however , the preference is restored when the SH 2 domain of Src is introduced into Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Mutational analysis revealed that the src homology 2 domain was essential for the phosphorylation of CrkII by EGF receptor but not by c Abl , arguing that these kinases phosphorylate CrkII by different phosphorylation mechanisms . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Two broad specificity biotinylated PTK peptide substrates were identified with optimum kinetic properties , allowing members from eight distinct classes of enzymes , including transmembrane ( epidermal growth factor receptor ( EGFR ) , fibroblast growth factor receptor , insulin receptor , and platelet derived growth factor receptor ) and cytoplasmic ( p 43 ( abl ) , p 56 ( lck ) , p 60 ( src ) , and p 93 ( fes ) ) PTKs , to be analyzed . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Whereas the ABI 1 gene bears no homology with the partner genes of MLL previously described , the ABI 1 protein exhibits sequence similarity to protein of homeotic genes , contains several polyproline stretches , and includes a src homology 3 ( SH 3 ) domain at the C terminus that is required for binding to Abl proteins in mouse Abi 1 protein . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In 32D myeloid cells and Rat 1 fibroblasts transformed by p 210 ( bcr abl ) , p 62 ( dok ) is tyrosine phosphorylated and co immunoprecipitates with Bcr Abl , RasGAP , and CrkL , a Src homology 2 ( SH 2 ) and SH 3 domain containing adaptor protein . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Tyrosine phosphorylated mammalian Disabled can recruit nonreceptor tyrosine kinases , such as src and abl , to the cytoplasmic tails of the receptors to which it binds , suggesting a molecular pathway by which receptor / ligand interaction on the cell surface could generate an intracellular signal . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Using this strategy , we have constructed highly efficient GFP based substrates for Src , c Abl , protein kinase A , and protein kinase C betaII protein kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We describe here the purification and mass spectrometric identification of a 155 kD tyrosine phosphorylated protein associated with src homologous and collagen gene ( SHC ) from p 210 ( bcr / abl ) expressing hematopoietic cells as SHIP 2 , a recently reported , unique SH 2 domain containing protein closely related to phosphatidylinositol polyphosphate 5 phosphatase SHIP . ^^^ Interestingly , SHIP 2 was found to selectively bind to the SH 3 domain of ABL , whereas SHIP 1 selectively binds to the SH 3 domain of Src . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Signal transducer and activator of transcription ( STAT ) 5 activation by BCR / ABL is dependent on intact Src homology ( SH ) 3 and SH 2 domains of BCR / ABL and is required for leukemogenesis . ^^^ We show here that BCR / ABL induces phosphorylation and activation of STAT 5 by a mechanism that requires the BCR / ABL Src homology ( SH ) 2 domain and the proline rich binding site of the SH 3 domain . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The compound was selective toward other protein kinases : the Src IC 50 value was lower than those for Cdc 2 ( > 500 fold ) , epidermal growth factor ( EGF ) receptor ( 7 . 5 fold ) , and vascular endothelial growth factor receptor ( > 50 fold ) , and for 5 Abl ( 15 fold ) and focal adhesion kinase ( Fak ) ( > 25 fold ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We screened 1680 spatially separated compounds of a diverse combinatorial library of 1 , 4 benzodiazepines for their ability to inhibit the kinase activity of protein tyrosine kinases Src , Yes , Abl , Lck , Csk , and fibroblast growth factor receptor . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In vitro , Tub is phosphorylated by purified insulin receptor kinase as well as by Abl and JAK 2 but not by epidermal growth factor receptor and Src kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We have accurately predicted several non Src family kinases that are moderately ( IC ( 50 ) approximately 1 microM ) inhibited by PP 1 , including c Abl and the MAP kinase p 38 . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Activation of the Abl tyrosine kinase in vivo by Src homology 3 domains from the Src homology 2 / Src homology 3 adaptor Nck . ^^^ We find that a construct encoding the first two Src homology 3 ( SH 3 ) domains of the Src homology 2 / SH3 adaptor protein Nck can activate c Abl in human 293T cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Abl is activated by growth factors and Src family kinases and has a role in the cellular response to PDGF . ^^^ To determine whether a link existed between activation of c Abl and members of the Src family , we examined c Abl kinase activity in cells that expressed oncogenic Src proteins . ^^^ We found that c Abl kinase activity was increased by 10 to 20 fold in these cells , and that Src and Fyn kinases directly phosphorylated c Abl in vitro . ^^^ Furthermore , overexpression of wild type Src potentiated c Abl activation by growth factors , and a kinase inactive form of Src reduced this activation , showing that Abl activation by growth factors occurs at least in part via activation of Src kinases . ^^^ These data identify c Abl as a downstream target of activated receptor tyrosine kinases and Src family kinases , and show for the first time that c Abl functions in the cellular response to growth factors . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We further established the specificity of the inhibition by showing that the SH 2 domains of several other tyrosine kinases ( Abl , Syk , and ZAP 70 ) , and the SH 3 domain of Src , were not inhibitory . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Using Src homology 3 domains from the proteins c Abl and c Crk as model systems , we show here that EPL can be performed in the presence of moderate concentrations of the chemical denaturant , guanidine hydrochloride , and the organic solvent dimethylsulfoxide . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A proline rich fragment of the NR2D subunit ( 2D ( 866 1064 ) ) bound to the Abl SH 3 domain but not to the SH 3 domains from Src , Fyn , Grb 2 , GAP , or phospholipase C gamma ( PLCgamma ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
EGFR , PDGFR ) or cytoplasmic ( Src , Abl ) tyrosine kinases are found mutated in a variety of human tumors . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The information of CR 50 antigen / Reelin is considered to be transmitted inside the neuroblasts to the `` disabled 1 ' ' , which is an adaptor protein to Src , Fyn , Abl tyrosine kinase . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The kinase deficient Src acts as a suppressor of the Abl kinase for Cbl phosphorylation . ^^^ The kinase activity of Abl is known to be regulated by a putative trans acting inhibitor molecule interacting with the Src homology ( SH ) 3 domain of Abl . ^^^ Here we report that the kinase deficient Src ( SrcKD ) directly inhibits the tyrosine phosphorylation of Cbl and other cellular proteins by Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Recent work shows that Hck , a member of the Src tyrosine kinase family with myeloid restricted expression , associates with and is activated by Bcr Abl . ^^^ To address the requirement for endogenous Src family kinase activation in Bcr Abl signaling , a kinase defective mutant of Hck was stably expressed in the cytokine dependent myeloid leukemia cell line DAGM . ^^^ These data show that Hck interacts with Bcr Abl through a complex mechanism involving kinase dependent and independent components and that interaction with Hck or other Src family members is essential for transformation signaling by Bcr Abl . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Recently a number of non receptor tyrosine kinases ( for example src and abl ) have been found to cause STAT phosphorylation . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The association of Grb 4 and Bcr Abl in intact cells was mediated by an src homology ( SH ) 2 mediated phosphotyrosine dependent interaction as well as an SH 3 mediated phosphotyrosine independent interaction . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The SH 3 domains of Src and Yes bound strongly , but that of Abl or phosphatidylinositol 3 kinase p 85 subunit did not . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The src homology 2 domain of Bcr / Abl is required for efficient induction of chronic myeloid leukemia like disease in mice but not for lymphoid leukemogenesis or activation of phosphatidylinositol 3 kinase . ^^^ The effect of mutations in the Src homology 2 ( SH 2 ) domain of the BCR / ABL oncogene on leukemogenesis was tested in a quantitative murine bone marrow transduction / transplantation assay that accurately models human Philadelphia positive B lymphoid leukemia and chronic myeloid leukemia ( CML ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Interestingly , deletion of the Src homology 3 domain complemented the deficiencies of the CC deleted Bcr Abl in inducing MPD in mice . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
In addition to BCR / ABL , STI 571 also inhibits 5 Abl , TEL / ABL , the native platelet derived growth factor ( PDGF ) beta receptor , and c KIT , but it does not inhibit SRC family kinases , c FMS , FLT 3 , the epidermal growth factor receptor , or multiple other tyrosine kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Deletion of the Src homology 3 domain and C terminal proline rich sequences in Bcr Abl prevents Abl interactor 2 degradation and spontaneous cell migration and impairs leukemogenesis . ^^^ We report here that the Src homology 3 domain and C terminal proline rich sequences of Bcr Abl are required for its binding to Abl interactor 2 as well as for the induction of Abl interactor 2 degradation . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
C Abl can be activated by several stimuli , including oxidative stress [ 3 ] , DNA damage [ 4 ] , integrin engagement [ 5 ] , growth factors , and Src family kinases [ 6 ] . ^^^ C Abl can be activated by several stimuli , including oxidative stress [ 3 ] , DNA damage [ 4 ] , integrin engagement [ 5 ] , growth factors , and Src family kinases [ 6 ] . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Growth factor receptor binding protein 2 ( Grb 2 ) plays a key role in signal transduction initiated by Bcr / Abl oncoproteins and growth factors , functioning as an adaptor protein through its Src homology 2 and 3 ( SH 2 and SH 3 ) domains . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Src homology 3 domains from several Src family PTKs including Lck , Fyn , Abl , and Src associate with ADAM 15 in vitro . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We present three genetically encoded fluorescent reporters for the tyrosine kinases Src , Abl , and epidermal growth factor ( EGF ) receptor . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Caveolin 1 is a substrate for nonreceptor tyrosine kinases including Src , Fyn , and Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This phage displayed library was screened by affinity selection for SH 3 domains that bind to the synthetic peptides , APTYPPPLPP and LSSRPLPTLPSP , which are peptide ligands for the human Abl or Src SH 3 domains , respectively . ^^^ Furthermore , we have used the information obtained by these in vitro evolution experiments to generate a scoring matrix that evaluates the probability that any SH 3 domain binds to the peptide ligands for the Abl and Src SH 3 domains . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Target assisted iterative screening reveals novel interactors for PSD 95 , Nedd 4 , Src , Abl and Crk proteins . ^^^ PDZ , WW and SH 3 domains from PSD 95 , Nedd 4 , Src , Abl and Crk proteins were used as targets . 12 novel putative and 2 previously reported interactions were identified in test screens . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of SSeCKS was apparent in cells deficient in Src , Fyn , Yes , or Abl tyrosine kinases or in NIH3T3 cells expressing a temperature sensitive 5 Src allele , but not in FAK deficient embryo fibroblasts . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The sensitivity of purified c Abl to STI 571 was increased by a dysregulating mutation ( P112L ) in the Src homology 3 domain of Abl but decreased by phosphorylation at the regulatory Tyr 393 . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Crosstalk between BCR / ABL oncoprotein and CXCR 4 signaling through a Src family kinase in human leukemia cells . ^^^ This chemokine signaling , which involves a Src related kinase and PI 3 kinase , appears to be a target for BCR / ABL , a fusion oncoprotein expressed only in leukemia cells . ^^^ These results define a Src tyrosine kinases dependent mechanism whereby BCR / ABL ( and potentially other oncoproteins ) dysregulates G protein coupled receptor signaling and function of mammalian precursors . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
On phosphorylation of Y 221 by Abelson ( Abl ) kinase , the Crk 2 adapter protein undergoes an intramolecular reorganization initiated by the binding of its own Src homology 2 ( SH 2 ) domain to the pY 221 site . ^^^ Conformational changes induced by phosphotyrosine recognition promote the binding of the Src homology 3 ( SH 3 ) domain of the Abl tyrosine kinase to a proline rich loop located between the betaD and betaE strands of the SH 2 domain ( DE loop ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Dual specific Src and Abl kinase inhibitors , PP 1 and CGP 76030 , inhibit growth and survival of cells expressing imatinib mesylate resistant Bcr Abl kinases . ^^^ These Bcr Abl mutants were then used to evaluate the binding mode and specificity of 2 compounds , PP 1 and CGP 76030 , originally characterized as Src kinase inhibitors . ^^^ Studies on the potential signaling mechanisms demonstrated that in cells expressing inhibitor resistant Bcr Abl mutants , PP 1 and CGP 76030 inhibited the activity of Src family tyrosine kinases and Akt but not signal transducer and activator of transcription 5 ( STAT 5 ) and JUN kinase ( Jnk ) . ^^^ The results suggest that the use of Src kinase inhibitors is a potential strategy to prevent or overcome clonal evolution of imatinib mesylate resistance in Bcr Abl ( + ) leukemia . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The Src family kinase Hck couples BCR / ABL to STAT 5 activation in myeloid leukemia cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The Src selective kinase inhibitor PP 1 also inhibits Kit and Bcr Abl tyrosine kinases . 4 amino 5 ( 4 methylphenyl ) 7 ( t butyl ) pyrazolo [ 3 , 4 d ] pyrimidine ( PP 1 ) was identified as an Src selective tyrosine kinase inhibitor and has been used extensively to investigate signaling pathways involving Src kinases , including events downstream of the stem cell factor ( SCF ) receptor c Kit . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We show that tyrosine phosphorylation of protein kinase D ( PKD ) at Y 463 in the Pleckstrin Homology ( PH ) domain is mediated by the Src and Abl tyrosine kinase signaling pathway , and that this is both necessary and sufficient to activate NF kappaB in response to oxidative stress . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
RESULTS : We found that Reelin induced Dab 1 tyrosine phosphorylation in neuron cultures is inhibited by acute treatment with pharmacological inhibitors of Src family , but not Abl family , kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
SKI 606 , a 4 anilino 3 quinolinecarbonitrile dual inhibitor of Src and Abl kinases , is a potent antiproliferative agent against chronic myelogenous leukemia cells in culture and causes regression of K 562 xenografts in nude mice . ^^^ Here we describe a p . o . active , dual Src / Abl kinase inhibitor with potent antiproliferative activity against CML cells in culture . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Arg heterodimers also involves direct binding of the Arg Src homology 3 domain to the C terminal region of c Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The interaction of the Bcr Abl tyrosine kinase with the Src kinase Hck is mediated by multiple binding domains . ^^^ We demonstrated recently that the Src kinase Hck interacts directly with Bcr Abl by a kinase independent mechanism . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This phosphorylation is mediated by a pathway that consists of the Src and Abl tyrosine kinases and occurs in response to stimulation with pervanadate and oxidative stress . ^^^ Mutational analysis revealed three tyrosine phosphorylation sites ( Tyr ( 432 ) , Tyr ( 463 ) , and Tyr ( 502 ) ) , which are regulated by the Src Abl pathway , and phosphorylation of only one of these ( Tyr ( 463 ) ) leads to PKD activation . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The c Abl tyrosine ( Tyr ) kinase is activated after platelet derived growth factor receptor ( PDGFR ) stimulation in a manner that is partially dependent on Src kinase activity . ^^^ However , the activity of Src kinases alone is not sufficient for activation of c Abl by PDGFR . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
This compound , PD 166326 , is a dual specificity TK inhibitor and inhibits src and abl in vitro with IC ( 50 ) s of 6 and 8 nM respectively . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
These activating phosphorylations have been proposed to occur either through autophosphorylation by Abl in trans or through phosphorylation of Abl by the Src nonreceptor tyrosine kinase . ^^^ We also find that the Src family kinase Hck phosphorylates the Abl and Arg activation loops , leading to an additional twofold stimulation of kinase activity . ^^^ The autoactivation pathway may allow Abl family kinases to integrate or amplify cues relayed by Src family kinases from cell surface receptors . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Inhibition of the activity of SRC and Abl tyrosine protein kinases by the binding of the Wiskott Aldrich syndrome protein . ^^^ We have found that WASP binds readily to a number of tyrosine protein kinases including the Src kinases and the Abl kinase when the proteins are coexpressed during transient transfection . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Our data suggest a novel mechanism for attenuation of Src kinase activity by Abl : stable tyrosine phosphorylation of a scaffolding protein , caveolin , and recruitment of Csk . ^^^ Paxillin , a substrate of both Abl and Src , organizes a similar regulatory complex . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Here , we have identified a direct binding between WRN and c Abl in vitro via the N terminal and central regions of WRN and the Src homology domain 3 of c Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Like Src and Abl PTKs , Btk contains a conserved cassette formed by SH 3 , SH 2 and protein kinase domains , but differs from them by the presence of an N terminal PH domain and the Tec homology region . ^^^ Taken together , our results indicate that , contrary to Src and Abl , Btk might not require an assembled conformation for the regulation of its activity . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Using a new in vitro embryonic slice culture system , we demonstrate that chemical inhibitors of Src family kinases and Abl , but not inhibitors of Abl alone , generate a reeler like malformation and that inhibitors of protein kinases C induce a malformation of cortical development that is also reminiscent of reeler . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We previously demonstrated that phosphorylation of PKD at Tyr 463 in the PH ( pleckstrin homology ) domain is mediated by the Src Abl pathway and that it is necessary for PKD activation and subsequent NF kappaB induction . ^^^ Tyrosine phosphorylation in the PH domain at Tyr 463 , mediated by the Src Abl pathway , which in turn facilitates the phosphorylation of Ser738 / Ser742 in the activation loop , mediated by the Src PKCdelta pathway . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Alkoxy 4 phenylamino 3 quinolinecar bonitriles as dual inhibitors of Src and Abl kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
One group of SH 2 domains ( Src , Fyn , Lck , Fgr , Abl , Crk , and Nck ) preferred sequences with the general motif pTyr hydrophilic hydrophilic Ile / Pro while another group ( SH 2 domains of p 85 , phospholipase C gamma , and SHPTP 2 ) selected the general motif pTyr hydrophobic 10 hydrophobic . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Requirement of Src kinases Lyn , Hck and Fgr for BCR ABL 1 induced B lymphoblastic leukemia but not chronic myeloid leukemia . ^^^ Here , we show that Bcr Abl activated the Src kinases Lyn , Hck and Fgr in B lymphoid cells . ^^^ BCR ABL 1 retrovirus transduced marrow from mice lacking all three Src kinases efficiently induced CML but not B ALL in recipients . ^^^ The biochemical target of CGP 76030 in leukemia cells was Src kinases , not Bcr Abl . ^^^ These results implicate Src family kinases as therapeutic targets in Ph ( + ) B ALL and suggest that simultaneous inhibition of Src and Bcr Abl kinases may benefit individuals with Ph ( + ) acute leukemia . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Using cell lines deficient in candidate tyrosine kinases , we found that the F actin depolymerization induced cortactin phosphorylation was mediated by the Fyn / Fer kinase pathway , independent of Src and c Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The relationship between the Src kinase Lyn and Bcl 2 expression was examined in chronic myelogenous leukemia cells ( K 562 and LAMA 84 ) displaying a Bcr / Abl independent form of imatinib mesylate resistance . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
AP 23464 , a potent adenosine 5 ' triphosphate ( ATP ) based inhibitor of Src and Abl kinases , displays antiproliferative activity against a human CML cell line and Bcr Abl transduced Ba / F3 cells ( IC ( 50 ) = 14 nM ; imatinib mesylate IC ( 50 ) = 350 nM ) . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
PLSCR 1 is a substrate of Src ( and Abl ) kinases , and transcription of the PLSCR 1 gene is regulated by the same growth factor receptor pathways in which PLSCR 1 potentiates afferent signaling . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The adaptor 3BP2 / SH3BP2 , an Abl Src homology domain 3 ( SH 3 ) binding and Syk kinases interacting protein , exhibits positive regulatory roles in T , natural killer ( NK ) , and basophilic cells . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The autoinhibited Src kinase structures reveal a mode of domain assembly used by other tyrosine kinases outside the Src family , including Abl and likely Tec family kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
By contrast , in erythroid cells , inhibitors of Src family and c Abl tyrosine kinases ( tyrphostin AG 82 , PP 2 , imatinib ) enhanced protein kinase C ( PKC ) dependent cell growth and antagonized thrombin promoted apoptosis by specifically stimulating PKCbeta activity . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
A recent crystal structure of the Abl N terminal region has revealed autoinhibitory interactions among the Src homology 3 ( SH 3 ) , SH 2 , and kinase domains . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
For example , the kinases Src and Abelson ( Abl ) were originally identified as oncogenes and were later characterized as important proteins for signal transduction in various cell types , including lymphocytes . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The pyridopyrimidinones are a potent class of inhibitors of c Abl kinase and Bcr Abl kinase , the causative fusion protein in chronic myelogenous leukemia and Src family kinases . ^^^ These results suggest that pyridopyrimidinone radiotracers may be useful in imaging Abl , Bcr Abl or Src expressing malignancies . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
SKI 606 , a Src / Abl inhibitor with in vivo activity in colon tumor xenograft models . ^^^ Therefore , we examined the activity of SKI 606 , a potent inhibitor of Src and Abl kinases , against colon tumor lines in vitro and in s . c . tumor xenograft models . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The cytoplasmic tyrosine kinase Abl is a Src substrate required for platelet derived growth factor ( PDGF ) receptor signaling leading to Myc expression and DNA synthesis . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Each of these residues is targeted by one or more specific kinases : Y 142 by Fyn , Fer and cMet ; Y 489 by Abl ; and Y 654 by Src and the epidermal growth factor receptor . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
The challenge for the future is to improve current clinical results with kinase inhibitors in CML , developing strategies that can eradicate residual disease and overcome or prevent resistance . ' Dual ' Src and Abl kinase inhibitors are an attractive class of compounds , since ( a ) these molecules are able to bind Bcr Abl with less stringent conformational requirements with respect to imatinib , therefore allowing for efficient inhibition of several , resistance associated mutant forms of Bcr Abl ; ( b ) Src kinases have been shown to be involved in Bcr Abl mediated leukemogenesis as well as upregulated in some patients resistant to imatinib . ^^^ Here , we review the development , the mode of action and the preclinical or early clinical evaluation of several novel dual Src and Abl kinase inhibitors . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
We also report that overexpression of e3B1 in NIH3T3 / EGFR cells renders EGF induced activation of Rap 1 completely dependent on Src tyrosine kinases but not on c Abl . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
An Src homology domain 2 kinase linker region exists in all Src , Abl , ZAP70 / Syk and Btk / Tec non receptor tyrosine kinase families . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Dasatinib ( BMS 354825 ) , a dual SRC / ABL kinase inhibitor , inhibits the kinase activity of wild type , juxtamembrane , and activation loop mutant KIT isoforms associated with human malignancies . ^^^ Dasatinib ( formerly BMS 354825 ) is a small molecule , ATP competitive inhibitor of SRC and ABL tyrosine kinases with potency in the low nanomolar range . ^^^ Some small molecule SRC / ABL inhibitors also have potency against WT KIT kinase . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Animal non receptor tyrosine kinases , SRC , ABL , LYN , FES , SEK , KIN and RAS have structural relationship with putative plant tyrosine kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Abl collaborates with Src family kinases to stimulate actin based motility of vaccinia virus . ^^^ Using cell lines deficient in Src , Fyn and Yes , we demonstrate that multiple Src family members can stimulate vaccinia induced actin polymerization and also uncover a role for Abl family kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Dasatinib [ BMS 354825 ] is an orally active , small molecule , dual inhibitor of both SRC and ABL kinases that is under development with Bristol Myers Squibb for the treatment of patients with chronic myelogenous leukaemia ( CML ) and imatinib acquired resistance / intolerance . ^^^ One of the possible causes of imatinib acquired resistance is associated with increased expression of the SRC related kinase Lyn and loss of BCR ABL dependence arising from sequence mutations . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Therefore , tyrosine phosphorylation dependent signaling involving receptor tyrosine kinases , mitogen activated protein kinases , Abl , Src , and Pyk 2 is known to be initiated or amplified by reactive oxidants . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Recently , small molecule , combined SRC and ABL 1 inhibitors have been developed and entered into clinical trials . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Dasatinib ( BMS 354825 ) , which inhibits Abl and Src family kinases , is another promising new clinical candidate for CML that has shown good efficacy in CML patients . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Previously , we showed that Abl kinases are activated downstream of growth factor receptors , Src family kinases , and phospholipase Cgamma 1 ( PLCgamma 1 ) in fibroblasts and influence growth factor mediated proliferation , membrane ruffling , and migration . ^^^ In this study , we found that Abl kinases are constitutively activated , in highly invasive breast cancer cell lines , downstream of deregulated ErbB receptors and Src kinases . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
Activity of dual SRC ABL inhibitors highlights the role of BCR / ABL kinase dynamics in drug resistance . ^^^ Because the active forms of ABL and SRC resemble one another , we tested two dual SRC ABL kinase inhibitors , AP 23464 and PD 166326 , against 58 imatinib resistant ( IM ( R ) ) BCR / ABL kinase variants . ^^^ Our data illustrate how conformational dynamics of the ABL kinase accounts for the activity of dual SRC ABL inhibitors against IM ( R ) mutants and provides a rationale for combining conformation specific inhibitors to suppress resistance . . ^^^
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
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Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA
Interacting proteins: P12931 and P00519	Pubmed	SVM Score :0.0
NA