| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| Double label immunofluorescent microscopy with the anti Tpr antibody and an antibody to the previously characterized nuclear pore complex protein nup 153 confirms that Tpr is localized to the nuclear pore complex . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| Major binding sites for the nuclear import receptor are the internal nucleoporin Nup 153 and the adjacent nuclear filament protein Tpr . ^^^ By immunoprecipitation , we find the nucleoporin Nup 153 and the pore associated filament protein Tpr , previously shown to reside at distinct sites on the intranuclear side of assembled pores , are each in stable subcomplexes with importin alpha and beta in Xenopus egg extracts . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| In a search for proteins that moor Tpr to the NPC , we have combined the RNAi approach with affinity chromatography and yeast two hybrid interaction studies , leading to the identification of nucleoporin Nup 153 as the binding partner for Tpr . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| Using both conventional and digital confocal immunofluorescence microscopy we have been able to define a time course of post mitotic assembly for a group of NPC components ( CAN / Nup214 , Nup 153 , POM 121 , p 62 and Tpr ) relative to the integral nuclear membrane protein LAP 2 and the NPC membrane glycoprotein gp 210 . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| To date , it has been possible to define a time course of postmitotic assembly for a group of NPC components ( CAN / Nup214 , Nup 153 , POM 121 , p 62 and Tpr ) relative to the integral inner nuclear membrane protein LAP 2 and the NPC membrane glycoprotein gp 210 . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| Taken together , these results showed that emerin , LBR , and several NPC components ( RanBP 2 , Nup 153 , p 62 ) , but not Tpr , reconstitute around chromosomes very early in telophase prior to the recovery of nuclear import activity . . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| Four nucleoporins , Nup 153 , RanBP 2 , Nup 214 and Tpr are cleaved by caspases during apoptosis . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| Using light and EM immunolocalization , we determined that mammalian Tpr is concentrated within the nuclear basket of the pore complex in a distribution similar to Nup 153 and Nup 98 . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| By postembedding immunoelectron microscopy , we have mapped the positions of several human NPC proteins relative to the NPC core and its associated basket , including Nup 93 , Nup 96 , Nup 98 , Nup 107 , Nup 153 , Nup 205 , and the coiled coil dominated 267 kDa protein Tpr . ^^^ To further assess their contributions to NPC and basket architecture , the genes encoding Nup 93 , Nup 96 , Nup 107 , and Nup 205 were posttranscriptionally silenced by RNA interference ( RNAi ) in HeLa cells , complementing recent RNAi experiments on Nup 153 and Tpr . ^^^ We show that Nup 96 and Nup 107 are core elements of the NPC proper that are essential for NPC assembly and docking of Nup 153 and Tpr to the NPC . ^^^ Nup 93 and Nup 205 are other NPC core elements that are important for long term maintenance of NPCs but initially dispensable for the anchoring of Nup 153 and Tpr . ^^^ Immunogold labeling for Nup 98 also results in preferential labeling of NPC core regions , whereas Nup 153 is shown to bind via its amino terminal domain to the nuclear coaxial ring linking the NPC core structures and Tpr . ^^^ |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49790 and P12270 |
Pubmed |
SVM Score :0.0 |
| NA |
|