| Pathways downstream of Shc and Grb 2 are required for cell transformation by the tpr Met oncoprotein . ^^^ Utilizing this mutant , together with additional Tpr Met mutants containing Tyr to Phe substitutions , we have demonstrated that transformation of Fr3T3 fibroblasts by the Tpr Met oncoprotein is dependent upon pathways downstream of Shc and Grb 2 and that pathways downstream of phosphatidylinositol 3 ' kinase , phospholipase Cgamma , and SHPTP2 / Syp are insufficient for transformation . . ^^^ |