| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.50259938 |
| This protein binds poly ( A ) with a slightly lower affinity as compared to PABP 1 . 0.50259938^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :1.004793 |
| Furthermore , the recombinant PABP was expressed and purified as a functional protein as indicated by its ability to bind to poly ( A ) ( + ) Sepharose and its ability to enhance the translation of adenylated messages in vitro . 1.004793^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.76070102 |
| PABP binds poly ( A ) cooperatively with a packing density of one PABP molecule per 25 adenosine residues . 0.76070102^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.77605302 |
| We have previously identified poly ( A ) binding protein 1 ( PABP 1 ) as a ligand for paxillin and shown that the paxillin PABP 1 complex undergoes nucleocytoplasmic shuttling . 0.77605302^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.90274681 |
| Consistent with this conclusion , UV cross linking results obtained using intact cells reveal that PABPC 1 binds directly to pre mRNA poly ( A ) tails in vivo . 0.90274681^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.56693096 |
| In contrast , Paip 2 strongly inhibits translation by promoting dissociation of PABP from poly ( A ) and by competing with eIF4G for binding to PABP . . 0.56693096^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Included within this group is the poly ( A ) binding protein ( PABP ) mRNA . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Recent studies suggest a role for poly ( A ) binding protein ( PABP ) in translation initiation . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The wheat poly ( A ) binding protein functionally complements pab 1 in yeast . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate the serum stimulated co isolation of the poly ( A ) binding protein ( PABP ) with the eIF4F complex . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This domain is contained in homologs of the poly ( A ) binding protein PABP and the ubiquitin protein ligase HYD . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) was also found to co purify with VPg Pro . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We found that Apc 5 binds the poly ( A ) binding protein ( PABP ) , which directly binds the IRES element . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Biochemical investigations have shown a specific interaction of poly ( A ) binding protein ( PABP ) with the DLS . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) was identified as a partner of K10 / 10 . 1 protein . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Computer assisted comparison reveals that one sequence motif of 30 amino acid residues is very similar to a region conserved in the C terminal part of eukaryotic poly ( A ) binding proteins ( PABP ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) is the major mRNA binding protein in eukaryotes , and it is essential for viability of the yeast Saccharomyces cerevisiae . ^^^ We produced different parts of the S . cerevisiae PABP and studied their binding to poly ( A ) and other ribohomopolymers in vitro . ^^^ Specific poly ( A ) binding activity was found only in the two amino terminal RBDs ( I / II ) which , interestingly , are dispensable for viability of yeast cells , whereas the activity that is sufficient to rescue lethality of a PABP deleted strain is in the carboxyl terminal RBDs ( III / IV ) . ^^^ We conclude that the PABP is a multifunctional RNA binding protein that has at least two distinct and separable activities : RBDs I / II , which most likely function in binding the PABP to mRNA through the poly ( A ) tail , and RBDs III / IV , which may function through binding either to a different part of the same mRNA molecule or to other RNA ( s ) . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| However , more recent studies demonstrate directly that poly ( A ) +mRNAs can initiate translation more efficiently than poly ( A ) mRNAs , and indicate that this effect is : ( 1 ) targeted to the formation of 80S initiation complexes , and ( 2 ) likely to be mediated by the cytoplasmic PABP . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This is 50 times more than required to bind all the poly ( A ) in the egg based on the binding stoichiometry of 1 PABP per 27 adenosine residues . ^^^ We found that density gradient centrifugation strips PABP from poly ( A ) and therefore underestimates the amount of PABP complexed to poly ( A ) + RNA in cell homogenates . ^^^ However , large pore gel filtration chromatography could be used to separate intact poly ( A ) PABP complexes from free PABP . ^^^ Using the gel filtration method , we found that the threefold increase in poly ( A ) content of the egg after fertilization is paralleled by an approximate fivefold increase in the amount of bound PABP . ^^^ Furthermore , both translated and nontranslated poly ( A ) + RNAs appear to be complexed to PABP . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Cold sensitive mutations in the SPB genes ( spb 1 spb7 ) of Saccharomyces cerevisiae suppress the inhibition of translation initiation resulting from deletion of the poly ( A ) binding protein gene ( PAB 1 ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| By two dimensional gel analysis and Western blot analysis the p 72 protein identified by PAb 6 is indistinguishable from the 72 , 000 mol w protein PABP associated with the poly ( A ) + tract of cytoplasmic messenger RNA molecules . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Using an in vitro mRNA decay system , we investigated how poly ( A ) and its associated poly ( A ) binding protein ( PABP ) affect mRNA stability . ^^^ Cell extracts used in the decay reactions were depleted of functional PABP either by adding excess poly ( A ) competitor or by passing the extracts over a poly ( A ) Sepharose column . ^^^ In contrast , the decay rates of nonpolyadenylated mRNAs were unaffected by PABP , indicating that both the poly ( A ) and its binding protein were required for maintaining mRNA stability . ^^^ The poly ( A ) tract was the first mRNA segment to be degraded in PABP depleted reactions , confirming that the poly ( A ) PABP complex was protecting the 3 ' region from nucleolytic attack . ^^^ A model is proposed to explain how the stability of an mRNA could be affected by the stability of its poly ( A ) PABP complex . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A 72 , 000 mol wt protein designated PABP binds to the poly ( A ) + track of messenger RNAs with high affinity and has been suggested to play an important role in mRNA metabolism in eucaryotic cells . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Depletion of the essential poly ( A ) binding protein ( PAB ) in S . cerevisiae by promoter inactivation or by the utilization of a temperature sensitive mutation ( pab 1 F364L ) results in the inhibition of translation initiation and poly ( A ) tail shortening . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| It is important to stress that additional genetic and biochemical tests are necessary to characterize how PABP interacts with mRNA in cells and to determine whether the poly ( A ) protection hypothesis is accurate . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The RNP consensus sequence was also found in the yeast poly ( A ) binding protein ( PABP ) , the heterogeneous nuclear RNA binding proteins A 1 and A 2 , and the pre rRNA binding protein C 23 . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have used synthetic peptide to study a conserved RNA binding motif in eukaryotic poly ( A ) binding protein ( PABP ) as well as its functions . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) , in a complex with the 3 ' poly ( A ) tail of eukaryotic mRNAs , plays important roles in the control of translation and message stability . ^^^ We show that the endogenous inactive PABP mRNA in rabbit reticulocytes can be specifically stimulated by addition of low concentrations of poly ( A ) and that this stimulation is also observed with in vitro transcribed human PABP mRNA . ^^^ By deleting the A rich region from the leader of human PABP mRNA and adding it upstream of the initiator AUG in a reporter mRNA we show that the adenylate tract is sufficient and necessary for mRNA repression and poly ( A ) mediated activation in the reticulocyte cell free system . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here we examine the association between the 70 kDa poly ( A ) binding protein ( PABP ) and stored ( RNP ) and polysomal mRNAs during mammalian male germ cell development . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have characterized the biochemical properties of a 66 kDa poly ( A ) binding protein ( PABP 1 ) in the protozoan Trypanosoma cruzi and isolated two classes of cDNAs encoding the protein . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) , a conserved protein that binds to the 3 ' poly ( A ) tail on mRNAs in eukaryotic cells , has been implicated in the regulation of mRNA stability and translation . ^^^ Comparison of the binding of PABP 1 and PABPt to poly ( A ) Sepharose in vitro revealed subtle differences , even though PABPt contains substitutions for highly conserved aromatic amino acids that are thought to be necessary for binding to poly ( A ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) binds to the messenger ( mRNA ) 3 ' poly ( A ) tail found on most eukaryotic mRNAs and together with the poly ( A ) tail has been implicated in governing the stability and the translation of mRNA . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| However , other mRNAs ( TCM 1 , PAB 1 , ACT 1 , and HTB 2 ) accumulate as poly ( A ) deficient ( A < approximately 25 ) transcripts as defined by an inability to bind oligo ( dT ) cellulose . ^^^ RNase mapping of transcripts from pap 1 1 cells revealed PAB 1 mRNA to be poly ( A ) whereas TCM 1 exists as equal amounts of poly ( A ) and poly ( A ) + mRNA 60 min after shift . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| As the binding of poly ( A ) binding protein 1 ( PAB 1 ) to the poly ( A ) tail of mRNAs is involved in both the stability and translation of cytoplasmic mRNAs , we investigated whether Rev might influence the association of PAB 1 with cytoplasmic HIV 1 RNAs . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) is highly susceptible to proteolysis during cell lysis of Drosophila tissue culture cells unless substantial amounts of proteolysis inhibitors are included in the extraction buffer . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We constructed a phylogenetic tree for the RNA binding domains , including those of poly ( A ) binding protein ( PABP ) , splicing factors , chloroplast RNPs , hnRNPs , snRNP U 1 70K , nucleolin and Drosophila sex determinants . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) is considered an essential component of a eukaryotic cell ; deletion of the PABP coding gene in yeast leads to a lethal phenotype . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The 70 kDa poly ( A ) binding protein 1 ( PAB 1 ) was obtained in different fractions of the same preparation . ^^^ Whereas PAB 2 stimulated poly ( A ) polymerase , PAB 1 was an inhibitor . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Localization of the human poly ( A ) binding protein gene ( PAB 1 ) to chromosomal regions 3q22 q 25 , 12q13 q 14 , and 13q12 q 13 by in situ hybridization . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Two classes of cDNAs encoding the Drosophila melanogaster ( poly ( A ) binding protein ( PABP ) , which differ in length due to different positions of their respective 3 ' ends , were isolated by screening an embryonic cDNA library . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| PABP , an inducible poly ( A ) binding protein detected in activated human T cells . ^^^ The poly ( A ) binding protein ( PABP ) binds to the poly ( A ) tail present at the 3 ' ends of most eukaryotic mRNAs . ^^^ In most tissues , poly ( A ) binding activity is likely to be the result of the combined effects of constitutively expressed PABP and iPABP , whose expression is subject to more complex regulation . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Subsets of the four RNA binding domains ( RBD 1 to 4 ) in the Xenopus poly adenylate binding protein ( PABP ) have distinct affinities and specificities for RNA . ^^^ RBDs 3 and 4 cooperate with the C terminus to gain poly ( A ) organizing activity , i . e . the ability to form an RNP with multiple , regularly spaced copies of PABP on a poly ( A ) substrate . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) is important for translation of eukaryotic mRNA and may be involved in shortening of its poly ( A ) tract . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Although its precise function remains to be elucidated , the highly conserved poly ( A ) binding protein 1 ( PABP ) mediates poly ( A ) dependent events in translation initiation and mRNA stability . ^^^ Xenopus oocytes contain less than one PABP per poly ( A ) binding site suggesting that the translation of maternal mRNAs could be either limited by or independent of PABP . ^^^ In contrast to a yeast PABP dependent poly ( A ) nuclease , PABP inhibits Xenopus oocyte deadenylase in vitro . ^^^ These results indicate that maturation specific deadenylation in Xenopus oocytes is facilitated by a low level of PABP consistent with a primary function for PABP to confer poly ( A ) stability . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| These observations suggest that Pabp may not require interaction with poly ( A ) tails during translation . ^^^ Further treatment of polyribosome lysates with agents known to differentially disrupt components of polyribosomes indicated that Pabp may require contact with some RNA component of the polyribosome , which could be either non poly ( A ) rich sequences of the translated mRNA or possibly a component of the ribosome . ^^^ These findings suggest that Pabp may possess the ability to bind to ribosomes independently of its interaction with poly ( A ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Furthermore , PAB 5 is capable of rescuing a PABP deficient yeast strain by partially restoring both poly ( A ) shortening and translational initiation functions of PABP . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Although inducers do not alter initiation factor modifications , characterization of their effect on mRNA translation provides evidence that this is mediated by the poly ( A ) binding protein ( PABP ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Maternal RNAs encoding poly ( A ) binding protein ( PABP ) , Vg 1 and Xcat 2 were associated with large complexes that , in contrast to polysomes , were not dissociated in magnesium free buffer . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here we describe one of these genes , fabM , which is predicted to encode a poly ( A ) binding protein ( PABP ) that is constitutively expressed and is essential for viability . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here we show that poly ( A ) binding protein 1 ( PABP 1 ) binds preferentially to INS 1 within the p 17 ( gag ) mRNA , but not to a mutated mRNA in which INS 1 function is eliminated . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Under these reaction conditions , DAN was also stimulated by the cytoplasmic poly ( A ) binding protein 1 ( PAB 1 ) . ^^^ At either salt concentration DAN and PAB 1 reconstituted poly ( A ) shortening with the same pattern of intermediates seen in cytoplasmic extract . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We report here that the cap associated eukaryotic initiation factors ( eIFs ) , 1 . e . the two isoforms of the cap binding complex ( eIF 4F and eIF iso4F ) and eIF 4B , bind to the poly ( A ) binding protein ( PABP ) both in the presence and absence of poly ( A ) RNA . ^^^ The interactions between PABP and eIF 4F , eIF iso4F , and eIF 4B were measured in the absence of poly ( A ) RNA using far Western analysis and confirmed by direct fluorescence titration studies . ^^^ The functional consequence of the interaction between these initiation factors and PABP was examined using RNA binding assays and RNA mobility shift analysis . eIF 4F , eIF iso4F , and eIF 4B promoted PABP activity through a shift in its equilibrium affinity for poly ( A ) . eIF iso4G , the large subunit of eIF iso4F , was the subunit responsible for the interaction between eIF iso4F and PABP and was the subunit that promoted PABP RNA binding activity . ^^^ These results suggest that the interaction between PABP and eIF 4B and eIF iso4G may be involved in mediating the functional co dependence observed between the cap and the poly ( A ) tail during translation . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Yeast Pab 1 interacts with Rna 15 and participates in the control of the poly ( A ) tail length in vitro . ^^^ In Saccharomyces cerevisiae , the single poly ( A ) binding protein , Pab 1 , is the major ribonucleoprotein associated with the poly ( A ) tails of mRNAs in both the nucleus and the cytoplasm . ^^^ Extracts from a thermosensitive pab 1 mutant and from a wild type strain immunoneutralized for Pab 1 showed normal cleavage activity but a large increase in poly ( A ) tail length . ^^^ Our data indicate that Pab 1 is also a part of the 3 ' end RNA processing complex and thus participates in the control of the poly ( A ) tail lengths during the polyadenylation reaction . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Extracts from strains where the PAB 1 gene is mutated or deleted are active for cleavage but generate transcripts bearing abnormally long poly ( A ) tracts . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Recently , the poly ( A ) binding protein ( PABP ) and cap binding protein , eIF 4F , were found to interact [ Le et al . ( 1997 ) J . ^^^ This effect was examined by fluorescence energy transfer , and it was determined that the PABP / eIF 4F complex could bind both poly ( A ) and 5 ' cap simultaneously . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The mRNA 3 ' polyadenylate tail and the associated poly ( A ) binding protein ( PABP ) also regulate translational initiation , probably by interacting with the 5 ' end of the mRNA . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have studied the intracellular localization of poly ( A ) binding protein 1 ( PABP 1 ) by indirect immunofluorescence as well as by tagging with the green fluorescent protein ( GFP ) in living cells . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here we report that a mutation in the SPB 8 ( YJL124c ) gene , initially identified as a suppressor mutation of a poly ( A ) binding protein ( PAB 1 ) gene deletion , stabilizes the mRNA cap structure . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) interacts with the cap associated eukaryotic initiation factor ( eIF ) 4G ( in yeast and plants ) and eIF4B ( in plants ) , a functional consequence of which is to increase the affinity of PABP for poly ( A ) and to increase the affinity of the cap binding complex , eIF4F ( of which eIF4G is a subunit ) for the 5 ' cap structure . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The mouse gene encoding the testis specific isoform of Poly ( A ) binding protein ( Pabp 2 ) is an expressed retroposon : intimations that gene expression in spermatogenic cells facilitates the creation of new genes . ^^^ The gene encoding the testis specific isoform of mouse poly ( A ) binding protein ( Pabp 2 ) has been isolated and sequenced . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that the amount of poly ( A ) binding protein ( PABP ) present in eIF4F complexes decreases during rotavirus infection , even though eIF4A and eIF4E remain unaffected . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| One key factor associated with this 3 ' end structure is the poly ( A ) binding protein ( Pab1p ) encoded by the PAB 1 gene in Saccharomyces cerevisiae . ^^^ The suppression of pab1Delta by pbp1Delta appears to be different from that mediated by other pab 1 suppressors , since disruption of PBP 1 does not alter translation rates , affect accumulation of ribosomal subunits , change mRNA poly ( A ) tail lengths , or result in a defect in mRNA decay . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Recent evidence indicates that the highly conserved 70 kDa cytoplasmic poly ( A ) binding protein ( PABP ) participates directly in translation initiation . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This report examines the effect of poliovirus infection on a recently characterized 3 ' end translational stimulatory protein , poly ( A ) binding protein ( PABP ) . ^^^ PABP is involved in stimulating translation initiation in lower eukaryotes by its interaction with the poly ( A ) tail on mRNAs and has been proposed to facilitate 5 ' end 3 ' end interactions in the context of the closed loop translational model . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Translation of the mRNA for the poly ( A ) binding protein ( PABP ) may be autoregulated by the binding of PABP to the A rich segment of its 5 ' untranslated region ( UTR ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In yeast and plants , binding of eIF4G to poly ( A ) binding protein ( PABP ) was implicated in poly ( A ) dependent translation . ^^^ In an in vitro translation system , an N terminal fragment which includes the PABP binding site inhibits poly ( A ) dependent translation , but has no effect on translation of a deadenylated mRNA . ^^^ These results indicate that , in addition to a recently identified mammalian PABP binding protein , PAIP 1 , eIF4G binds PABP and probably functions in poly ( A ) dependent translation in mammalian cells . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) is an important regulator of gene expression that has been implicated in control of translation initiation . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein 1 ( PABPI ) is a highly conserved eukaryotic protein that binds mRNA poly ( A ) tails and functions in the regulation of translational efficiency and mRNA stability . ^^^ As a first step in our investigation of the role ( s ) of mRNA poly ( A ) tails in posttranscriptional gene regulation in Trypanosoma brucei , we have cloned the cDNA encoding PABPI from this organism . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Search for other GSPT binding proteins in yeast two hybrid screening system resulted in the identification of a cDNA encoding polyadenylate binding protein ( PABP ) , whose amino terminus is associating with the poly ( A ) tail of mRNAs presumably for their stabilization . ^^^ Interestingly , multimerization of PABP with poly ( A ) , which is ascribed to the action of its carboxyl terminal domain , was completely inhibited by the interaction with the amino terminal domain of GSPT . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Incubation of the RNA with erythroleukemia K 562 cytosolic extract results in deadenylation with distinct intermediates containing a periodicity of approximately 30 nucleotides , which is consistent with the binding of poly ( A ) binding protein ( PABP ) monomers . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Most eukaryotic mRNAs possess a 5 ' cap structure ( m ( 7 ) GpppN ) and a 3 ' poly ( A ) tail which promote translation initiation by binding the eukaryotic translation initiation factor ( eIF ) 4E and the poly ( A ) binding protein ( PABP ) , respectively . eIF4G can bridge between eIF4E and PABP , and through eIF 3 is thought to establish a link to the small ribosomal subunit . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) mRNA is subject to autoregulation through a 61 nucleotides long A rich sequence in its 5 ' untranslated region ( UTR ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have shown previously that the stimulation of cultured Xenopus kidney cells with serum resulted in the activation of protein synthesis , enhanced phosphorylation of eIF4E and increased binding of the adapter protein , eIF4G , and poly ( A ) binding protein ( PABP ) to eIF4E to form the functional initiation factor complex , eIF4F / PABP . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The cocrystal structure of human poly ( A ) binding protein ( PABP ) has been determined at 2 . 6 A resolution . ^^^ PABP recognizes the 3 ' mRNA poly ( A ) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization / degradation . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The promoter of the Poly ( A ) binding protein 2 ( Pabp 2 ) retroposon is derived from the 5 ' untranslated region of the Pabp 1 progenitor gene . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In humans , the poly ( A ) binding proteins ( PABPs ) comprise a small nuclear isoform and a conserved gene family that displays at least three functional proteins : PABP 1 , inducible PABP ( iPABP ) , and PABP 3 , plus four pseudogenes ( 1 , 2 , 3 , and PABP 4 ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The mammalian eukaryotic initiation factor 4GI ( eIF4GI ) may be divided into three roughly equal regions ; an amino terminal one third ( amino acids [ aa ] 1 to 634 ) , which contains the poly ( A ) binding protein ( PABP ) and eIF4E binding sites ; a middle third ( aa 635 to 1039 ) , which binds eIF4A and eIF 3 ; and a carboxy terminal third ( aa 1040 to 1560 ) , which harbors a second eIF4A binding site and a docking sequence for the Ser / Thr kinase Mnk 1 . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A cold sensitive allele for that gene ( referred to here as spb 1 1 ) had been previously isolated as a suppressor of a mutation affecting the poly ( A ) binding protein gene ( PAB 1 ) and a thermosensitive allele ( referred to here as spb 1 2 ) was isolated in a search for essential genes required for gene silencing in Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Instead , the amino terminal domain is capable of binding to the carboxy terminal domain of polyadenylate binding protein ( PABP ) , whose amino terminus is associating with the poly ( A ) tail of mRNAs , presumably for their stabilization . ^^^ Interestingly , multimerization of PABP with poly ( A ) , which is ascribed to the action of its carboxy terminal domain , was completely inhibited by the interaction with the amino terminal domain of GSPT . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Degenerate PCR has been used to clone the gene encoding the Leishmania homologue of poly ( A ) binding protein ( Lm PAB 1 ) , as an approach to the identification of trans acting factors involved in this atypical mode of eukaryotic gene expression . lmpab 1 is a single copy gene encoding a 63 kDa protein which shares major structural features but only 35 40 % amino acid identity with other PAB 1 sequences , including those of other trypanosomatids . ^^^ Lm PAB 1 binds poly ( A ) with high specificity and affinity but fails to complement a null mutation in Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The similar properties of Hfq and the PABPII poly ( A ) binding protein , which stimulates poly ( A ) tail elongation in mammals , indicates that similar mechanisms control poly ( A ) tail synthesis in prokaryotes and eukaryotes . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| It is suggested that formation of the double helix in the mRNA poly ( A ) tall provides the basis for such processes as polyadenylation termination , PAB 1 synthesis autoregulation , and stabilization of ARE containing mRNA by ELAV like proteins . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In the cytoplasm , a distinct protein ( PABP 1 ) binds to poly ( A ) tails and participates in mRNA translation and stability . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) interacts with the eukaryotic initiation factor ( eIF ) 4G ( or eIFiso4G ) , the large subunit of eIF4F ( or eIFiso4F ) to promote translation initiation . ^^^ In this study , we have purified the phosphorylated and hypophosphorylated isoforms of PABP from wheat to examine whether its phosphorylation state affects its binding to poly ( A ) RNA and its interaction with eIF4G , eIFiso4G , or eIF4B . ^^^ Phosphorylated PABP exhibited cooperative binding to poly ( A ) RNA even under non stoichiometric binding conditions , whereas multiple molecules of hypophosphorylated PABP bound to poly ( A ) RNA only after free poly ( A ) RNA was no longer available . ^^^ These data suggest that the phosphorylation state of PABP specifies the type of binding to poly ( A ) RNA and its interaction with its partner proteins . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Recent studies demonstrated that wheat germ poly ( A ) binding protein ( PABP ) interacted with translation eukaryotic initiation factor ( eIF ) iso4G and eIF4B , and these interactions increased the poly ( A ) binding activity of PABP ( Le , H . , Tanguay , R . ^^^ ATPase kinetic assays show , in the presence of poly ( U ) , PABP can increase the parameter ( k ( cat ) / K ( m ) ) by 3 . 5 fold with a 2 fold decrease of K ( m ) for the ( eIF4A + eIF iso4F ) complex . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation of UV cross linked proteins identified the 73 kDa protein as the cytoplasmic poly ( A ) binding protein ( PABP ) . ^^^ RNAs with shortened poly ( A ) tails exhibited less in vitro PABP binding , suggesting that decreased interactions with the protein may affect RNA replication . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The single yeast gene ( PAB 1 ) encoding poly ( A ) binding protein ( PABP ) has several roles in post transcriptional processes , including translation initiation and mRNA decay . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) tails of eukaryotic mRNAs are complexed with poly ( A ) binding protein ( PABP ) . ^^^ The poly ( A ) PABP complex is central to the efficient translation initiation and control of poly ( A ) tail length and is required in some pathways of mRNA decay . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The 5 ' cap and poly ( A ) tail of eukaryotic mRNAs work synergistically to enhance translation through a process that requires interaction of the cap associated eukaryotic initiation factor , eIF 4G , and the poly ( A ) binding protein , PABP . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Six genes were confirmed as three to ninefold upregulated in dauer larvae , after correction for mRNA load : genes encoding poly ( A ) binding protein ( PABP ) , heat shock proteins hsp 70 and hsp 90 , and three novel genes of uncertain function . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The role of 5 ' leader length , secondary structure and PABP concentration on cap and poly ( A ) tail function during translation in Xenopus oocytes . ^^^ Co injection of poly ( A ) binding protein ( PABP ) mRNA increased the function of the cap and poly ( A ) tail in promoting translation from poly ( A ) ( + ) but not poly ( A ) ( ) mRNAs , particularly for mRNAs containing secondary structure . ^^^ These data suggest that the length of a leader , the presence of secondary structure and the concentration of PABP determine the extent to which the cap and poly ( A ) tail regulate translation . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Maximal cap poly ( A ) cooperativity required the integrity of the eukaryotic initiation factor 4G poly ( A ) binding protein ( eIF4G PABP ) interaction , suggesting that synergy results from mRNA circularization . ^^^ These effects of poly ( A ) were also sensitive to disruption of the eIF4G PABP interaction , suggesting that 5 ' 3 ' end cross talk is functionally conserved between classical mRNAs and an IRES containing mRNA . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The influence of the poly ( A ) tail was mediated through the poly ( A ) binding protein ( PABP ) bound to the poly ( A ) tail , which can inhibit ErEN activity . ^^^ Conversely , the binding of PABP to the poly ( A ) tail was also augmented by alphaCP , implying that a stable higher order structural network is involved in stabilization of the alpha globin mRNA . ^^^ The data demonstrated a specific role for PABP in protecting the body of an mRNA in addition to demonstrating PABP ' s well characterized effect of stabilizing the poly ( A ) tail . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The eukaryotic initiation factor complex eIF4F , which in yeast contains the core components eIF4E and eIF4G , uses the cap as a binding site , serving as an initial point of assembly for the translation apparatus , and also binds the poly ( A ) binding protein Pab 1 . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) tail of eukaryotic mRNAs regulates translation and RNA stability through an association with the poly ( A ) binding protein ( PABP ) . ^^^ The levels of clam PABP are constant in early embryogenesis , although its ability to crosslink labelled poly ( A ) is ' masked ' shortly after fertilisation and remains so until the larval stage . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A poly ( A ) binding protein ( PABP ) with mol wt 72 , 000 has been purified from chickpea ( Cicer arietinum ) epicotyls by ammonium sulfate fractionation , Cibacron blue F 3 GA and poly ( A ) agarose chromatography . ^^^ This PABP is highly susceptible to proteolysis and upon degradation forms a polypeptide fragment of mol wt 21 , 000 which has an independent poly ( A ) binding activity . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Several cDNA clones representing a single copy of a poly ( A ) binding protein ( PABP ) gene were isolated from a cucumber ( Cucumis sativus L . ) leaf cDNA library . ^^^ Full length cucumber PABP cDNA was obtained using 5 ' RACE ; in vitro and Escherichia coli expressed PABP bound to poly ( A ) Sepharose and ZYMY RdRp with or without the presence of poly ( A ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Five mCRD associated proteins were identified : Unr , a purine rich RNA binding protein ; PABP , a poly ( A ) binding protein ; PAIP 1 , a poly ( A ) binding protein interacting protein ; hnRNP D , an AU rich element binding protein ; and NSAP 1 , an hnRNP R like protein . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Biochemical characterisation of cap poly ( A ) synergy in rabbit reticulocyte lysates : the eIF4G PABP interaction increases the functional affinity of eIF4E for the capped mRNA 5 ' end . ^^^ Using these systems , we demonstrate that synergy requires interaction between the poly ( A ) binding protein ( PABP ) and the eukaryotic initiation factor ( eIF ) 4F holoenzyme complex , which recognises the 5 ' cap . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We used green flourescent protein tagged SG associated RNA binding proteins ( specifically , TIA 1 and poly [ A ] binding protein [ PABP 1 ] ) to monitor SG assembly , disassembly , and turnover in live cells . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This effect is mediated by a bridging complex , composed of the poly ( A ) binding protein ( PABP ) , eIF4G , and the cap binding protein , eIF4E . ^^^ Paip 2 decreases the affinity of PABP for polyadenylate RNA , and disrupts the repeating structure of poly ( A ) ribonucleoprotein . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A novel ARE binding protein called ePAB ( for embryonic poly ( A ) binding protein ) has been purified from this extract by ARE affinity selection . ePAB exhibits 72 % identity to mammalian and Xenopus PABP 1 and is the predominant poly ( A ) binding protein expressed in the stage 6 oocyte and during Xenopus early development . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Mutations in ribosomal protein L 39 ( rpl 39 ) complement loss of function mutations in SIS 1 as well as PAB 1 ( poly ( A ) binding protein ) , suggesting a functional interaction between these proteins . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Seventeen mRNAs overexpressed in preleukemic thymuses were identified : mouse laminin binding protein ( p40 / 37LBP ) , E 25 protein , Rattus norvegicus clone BB . 1 . 4 . 1 , profilin , poly ( A ) binding protein ( PABP ) , mouse high mobility group protein 1 , topoisomerase 1 , clusterin , proteasome RC 1 subunit , rat prostatein C 3 and C 1 subunits ; two ESTs and four unknown genes . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Biochemical purification revealed that this protein is the multifunctional poly ( A ) binding protein ( PABP ) . ^^^ With lower affinities , PABP can also associate with non poly ( A ) sequences . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Two of the promoters were of viral origin : ( a ) SV 40 promoter ( simian virus early promoter ) and ( b ) CMV promoter ( cytomegalovirus early promoter ) ; two were of mammalian cell origin : ( c ) PABP promoter ( human poly ( A ) binding protein promoter ) and ( d ) S 16 promoter ( mouse ribosomal protein ( rp ) S 16 promoter ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) is an essential , well conserved , multifunctional protein involved in translational initiation , mRNA biogenesis , and degradation [ 1 5 ] . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The properties of two universal major proteins of cytoplasmic mRNP , p 50 and the poly ( A ) binding protein ( PABP ) , are summarized . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In this model , the transcript termini are brought together by interactions of poly ( A ) binding protein ( PABP ) with both the poly ( A ) tail and initiation factor eIF4G . ^^^ We considered the possibility that the cytosolic inhibitor blocked the interaction of PABP with the poly ( A ) tail or with eIF4G . ^^^ We found that neither of these interactions were inhibited , as shown by immunoprecipitation of PABP followed by quantitation of the poly ( A ) tail by reverse transcription PCR and of eIF4G by immunoblot analysis . ^^^ These data show that translational silencing of Cp requires interactions of three essential elements of mRNA circularization , poly ( A ) , PABP , and eIF4G . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Further equilibrium studies showed that poly ( A ) binding protein ( PABP ) enhanced the cap binding of eIFiso4F about 40 fold . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The interaction between eukaryotic translation initiation factor 4G ( eIF4G ) and the poly ( A ) binding protein ( PABP ) facilitates translational initiation of polyadenylated mRNAs . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This interaction is mediated , at least in part , by elF4G , which bridges the mRNA termini by simultaneous binding the poly ( A ) binding protein ( PABP ) and the cap binding protein , elF4E . ^^^ Here , we support this hypothesis by cleaving elF4G to separate the PABP binding site from the portion that promotes internal initiation . elF4G cleavage abrogates the stimulatory effect of poly ( A ) tail on translation . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Rotaviruses , the cause of life threatening diarrhea in humans and cattle , utilize a functional homolog of poly ( A ) binding protein ( PABP ) known as nonstructural protein 3 ( NSP 3 ) for translation of viral mRNAs . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A poly ( A ) binding protein ( PABP ) with mol wt 29 , 000 has been purified from chickpea ( Cicer arietinum ) epicotyl by ammonium sulfate fractionation and Cibacron blue F 3 GA chromatography , making a complex with poly ( A ) and elution of PABP poly ( A ) complex at 45 degrees C from oligo d ( T ) cellulose . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition to cleavage of eIF4GI , cleavage of eIF4GII and poly ( A ) binding protein ( PABP ) has been recently proposed to contribute to complete host translation shutoff ; however , the relative importance of eIF4GII and PABP cleavage has not been determined . ^^^ In addition , binding of PABP to poly ( A ) RNA stimulates 3C ( pro ) mediated cleavage and inhibits 2A ( pro ) mediated cleavage . ^^^ These results suggest that 3C ( pro ) plays a major role in processing PABP during virus infection and that the interaction of PABP with translation initiation factors , ribosomes , or poly ( A ) RNA may promote its cleavage by viral 2A and 3C proteases . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| EIF4G interacts with several other factors including eIF4E , which is a cap binding protein , and the poly ( A ) binding protein ( PABP ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| PABC domains are peptide binding domains found in poly ( A ) binding proteins ( PABP ) and are a subset of HECT family E 3 ubiquitin ligases ( also known as hyperplastic discs proteins ( HYDs ) ) . ^^^ In mammals , the PABC domain of PABP functions to recruit several different translation factors to the mRNA poly ( A ) tail . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Rotaviruses , segmented double stranded RNA viruses , co opt the eukaryotic translation machinery with the aid of nonstructural protein 3 ( NSP 3 ) , a rotaviral functional homolog of the cellular poly ( A ) binding protein ( PABP ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| On the basis of mobility change observed in RNP complexes when the A rich region is removed , we decided to examine poly ( A ) binding protein ( PABP ) as a candidate member of the RNP . ^^^ Given the ubiquitous role of poly ( A ) regions in the retrotransposition process , these data suggest that PABP may contribute to the SINE retrotransposition process . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate with a variety of experimental approaches that poly ( A ) binding protein ( PABP 1 ) , a regulator of translation initiation , binds to both RNAs in vitro and in vivo . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Analysis of KS 24 suggested that mRNA pool used in its construction originated from poly ( A ) binding protein ( PABP ) mRNP complexes , which are probably located in nuclear structures known as interchromatin granule clusters ( IGCs ) . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This function requires only the C domain that is homologous to the elongation factor ( EF ) 1alpha , while the N domain interacts with polyadenylate binding protein ( PABP ) , which binds the poly ( A ) tail of mRNA and associates with the eukaryotic initiation factor ( eIF ) 4G . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Biochemical purification revealed that this protein is the multifunctional poly ( A ) binding protein ( PABP ) . ^^^ With lower affinities , PABP can also associate with non poly ( A ) sequences . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| An interaction between human poly ( A ) binding protein ( PABP ) et human eRF 3 has been demonstrated using a double hybrid approach and in vitro assays . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The recruitment of Poly ( A ) Binding Protein ( PABP ) during poly ( A ) tail lengthening appears to be the key to translational activation during this period of development in Xenopus laevis . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A protein complex , termed the alpha complex , forms on the CRE and has been shown to contribute to stabilization of the mRNA by at least two mechanisms , first by interacting with the poly ( A ) binding protein ( PABP ) to prevent deadenylation , and second by protecting the mRNA from attack by an erythroid endoribonuclease . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| One of them , the cytoplasmic poly ( A ) binding protein PABP 1 , co migrated with a protein that showed prominent phosphorylation by recombinant MK 2 . ^^^ Phosphorylation by MK 2 was confirmed using PABP 1 purified by affinity chromatography on poly ( A ) RNA . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Eukaryotic poly ( A ) binding protein ( PABP ) is a ubiquitous , essential factor involved in mRNA biogenesis , translation , and turnover . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have identified these proteins as PABP ( poly ( A ) binding protein ) ( 69 kDa ) and YB 1 ( 50 kDa ) and demonstrated that titrating out of PABP by poly ( A ) strongly and specifically inhibits YB 1 mRNA cap ( + ) poly ( A ) ( ) translation in a cell free system . ^^^ Thus , PABP is capable of positively affecting YB 1 mRNA translation in a poly ( A ) tail independent manner . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The methylation of two known CARM 1 substrates , poly ( A ) binding protein ( PABP 1 ) and the transcriptional cofactor p 300 , was abolished in knockout embryos and cells . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) is a highly conserved eukaryotic protein whose synthesis is regulated at the post transcriptional level . ^^^ The binding of PABP to the poly ( A ) rich element found in the 5 ' untranslated region ( 5 ' UTR ) of PABP mRNA specifically inhibits its own translation . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The N domain of eRF 3 , which is not necessarily required for translation termination , interacts with the poly ( A ) binding protein PABP . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Identification of a C terminal poly ( A ) binding protein ( PABP ) PABP interaction domain : role in cooperative binding to poly ( A ) and efficient cap distal translational repression . ^^^ The poly ( A ) binding protein ( PABP ) , bound to the 3 ' poly ( A ) tail of eukaryotic mRNAs , plays critical roles in mRNA translation and stability . ^^^ PABP is composed of two parts : the highly conserved N terminus , containing 4 RNA recognition motifs ( RRMs ) responsible for poly ( A ) and eIF4G binding ; and the more variable C terminus , which includes the recently described PABC domain , and promotes intermolecular interaction between PABP molecules as well as cooperative binding to poly ( A ) . ^^^ Moreover , cooperative binding by multiple PABP molecules to poly ( A ) requires the C terminus , but not the PABC domain . ^^^ Altogether , our results identify a proline and glutamine rich linker located between the RRMs and the PABC domain as being strictly required for PABP / PABP interaction , cooperative binding to poly ( A ) and enhanced translational repression of reporter mRNAs in vitro and in vivo . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) tail shortening in mRNA , called deadenylation , is the first rate limiting step in eukaryotic mRNA turnover , and the polyadenylate binding protein ( PABP ) appears to be involved in the regulation of this step . ^^^ Here we identified and characterized a human PABP dependent poly ( A ) nuclease ( hPAN ) complex consisting of catalytic hPan 2 and regulatory hPan 3 subunits . hPan 2 has intrinsically a 3 ' to 5 ' exoribonuclease activity and requires Mg2+ for the enzyme activity . ^^^ Interestingly , the hPAN nuclease complex has a higher substrate specificity to poly ( A ) RNA upon its association with PABP . ^^^ Thus , the human PAN complex is a poly ( A ) specific exoribonuclease that is stimulated by PABP in the cytoplasm . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Retinoblastoma binding protein 1 ( RBBP 1 ) decreased by four times , Homo sapiens craniofacial development protein 1 ( CFDP 1 ) decreased by 2 . 4 times , DAP Kinase related apoptosis inducing protein Kinase 1 ( DRAK 1 ) decreased by 2 . 3 times , SKI interacting protein ( SKIP ) decreased by 2 . 2 times , and human poly ( A ) Binding protein ( PABP ) decreased by 2 . 1 times . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The addition of a poly ( A ) tail ( 24 nts ) to the 3 ( ' ) UTR permits the specific binding of the poly ( A ) binding protein ( PABP ) present in HeLa cell extracts , as well as the recombinant PABP . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Eukaryotic poly ( A ) binding protein ( PABP ) is a ubiquitous , essential cellular factor with well characterized roles in translational initiation and mRNA turnover . ^^^ Expression of PABP from Arabidopsis thaliana , PAB 3 , rescues an otherwise lethal phenotype of the yeast pab1Delta mutant , but it neither restores the poly ( A ) dependent stimulation of translation , nor protects the mRNA 5 ' cap from premature removal . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have previously reported that NS 1 interacts with the translation initiation factor eIF4GI , next to its poly ( A ) binding protein 1 ( PABP 1 ) interacting domain and that NS 1 and eIF4GI are associated in influenza virus infected cells . ^^^ Here we show that NS 1 , although capable of binding poly ( A ) , does not compete with PABP 1 for association with eIF4GI and , furthermore , that NS 1 and PABP 1 interact both in vivo and in vitro in an RNA independent manner . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The circularisation model of the polysome suggests that ribosome recycling is facilitated by 5 ' 3 ' interactions mediated by the cap binding complex eIF4F and the poly ( A ) binding protein , Pab 1 . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In protein synthesis , the PABC domain of PABP functions to recruit several translation factors possessing the PABP interacting motif 2 ( PAM 2 ) to the mRNA poly ( A ) tail . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) binds mRNA poly ( A ) tails and affects mRNA stability and translation . ^^^ These data suggest that iPABP and PABP may in some situations interact with other RNA regions in addition to the poly ( A ) tail . . ^^^ |
|
| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The 3 ' poly ( A ) tail of eukaryotic mRNAs and the poly ( A ) binding protein ( PABP ) play important roles in the regulation of translation . ^^^ Recently , a human PABP interacting protein , Paip 2 , which disrupts the PABP poly ( A ) interaction and consequently inhibits translation , was described . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have recently shown that poly ( A ) binding protein ( PABP ) is cleaved during poliovirus and Coxsackievirus infection by viral 3Cprotease and that 3Cprotease modification of a subset of PABP can result in significant translation inhibition . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Viral 3C protease ( 3Cpro ) cleaves poly ( A ) binding protein ( PABP ) and removes the C terminal domain ( CTD ) that interacts with several translation factors . ^^^ In HeLa cell translation extracts that exhibit cap poly ( A ) synergy , partial cleavage of PABP by 3Cpro inhibited translation of endogenous mRNAs and reporter RNA as effectively as complete cleavage of eIF4GI and eIF4GII by 2Apro . 3Cpro mediated translation inhibition was poly ( A ) dependent , and addition of PABP to extracts restored translation . ^^^ The data illustrate the importance of the CTD of PABP in poly ( A ) dependent translation in mammalian cells . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This phenomenon has been explained by the simultaneous binding of poly ( A ) binding protein ( PABP ) and a cap binding protein ( eIF4E ) to eIF4G that results in the circularization of the mRNA ( closed loop model ) . ^^^ Greater than 98 % of PABP can be depleted from extracts by preincubation with either of the PABP interacting proteins ( Paip 2 or Paip 1 ) coupled to beads , and these depleted extracts fail to support efficient translation of poly ( A ) + mRNAs . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Therefore , at least in this way , the pioneer round of translation is distinct from steady state translation . eIF4GI , poly ( A ) binding protein ( PABP ) 1 , eIF 3 , eIF4AI , and eIF2alpha coimmunopurify with both CBP 80 and eIF4E , which suggests that each factor functions in both modes of translation . ^^^ Consistent with roles for PABP 1 and eIF2alpha in the pioneer round of translation , PABP interacting protein 2 , which is known to destabilize PABP 1 binding to poly ( A ) and inhibit steady state translation , as well as inactive eIF2alpha , which is also known to inhibit steady state translation , also inhibit NMD . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We screened for proteins that associate with Mkt 1 and isolated Pbp 1 , a protein that is known to associate with Pab 1 , a poly ( A ) binding protein . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the poly ( A ) tail mediated regulation of mRNA decapping observed in humans can be recapitulated in vitro by the cytoplasmic poly ( A ) binding protein PABP through a direct and specific binding to the 5 ' end of capped mRNA . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We show that the viral 100k protein possesses a selective binding element for the 5 ' noncoding region ( 5 ' NCR ) of viral late mRNAs ( known as the tripartite leader ) , forms a complex with initiation factor eIF4G and poly ( A ) binding protein ( PABP ) , and strongly and selectively enhances the level of both factors and 40S ribosome subunits on viral mRNAs in polysomes . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition , eRF 3 regulates the termination coupled events , eRF 3 interacts with poly ( A ) binding protein ( Pab 1 ) and the surveillance factor Upf 1 to mediate normal and nonsense mediated mRNA decay . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In order to approach these questions , we have used RNA binding proteins such as ELAV / Hu , poly ( A ) binding protein ( PABP ) and cap binding protein ( eIF 4E ) as reporters of gene expression . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding proteins ( PABPs ) are central to the regulation of messenger RNA ( mRNA ) translation and stability ; however , the roles and contributions of different PABP family members in controlling gene expression are not yet fully understood . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The known poly ( A ) binding proteins can be divided into three distinct structural groups ( prototypes PABP 1 , PABPN1 / PABP2 and Nab2p ) and two functional families , showing that similar functions can be accomplished by differing structural units . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Enhancement of IRES mediated translation of the c myc and BiP mRNAs by the poly ( A ) tail is independent of intact eIF4G and PABP . ^^^ Moreover , the poly ( A ) tail stimulates IRES mediated translation even in the presence of PAIP 2 or following effective depletion of the poly ( A ) binding protein ( PABP ) from HeLa cell extracts . ^^^ The PABP eIF4G bridging complex that is critical for cap dependent translation is thus dispensable for the enhancement of the IRESs by the poly ( A ) tail . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Among proteins interacting specifically with both RNAs is the multifunctional poly ( A ) binding protein ( PABP ) consisting of four RNA recognition motifs ( RRMs ) and a C terminal auxiliary domain . ^^^ Quantitative binding studies with PABP deletion mutants demonstrate preferential binding of RRMs 3+4 even to poly ( A ) homopolymers , while RRMs 1+2 exhibit a lower affinity for those sequences . ^^^ An optimal interaction with both poly ( A ) and non poly ( A ) sequences is only achieved by full size PABP . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Using this approach we efficiently recovered and quantified RNAs bound to cytoplasmic poly ( A ) binding protein ( PABP ) and to nuclear human transformer 2 ( hTra 2 ) with minimal background . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Staufen SGs contain the poly ( A ) binding protein ( PABP ) , the RNA binding proteins HuR and TIAR , and small but not large ribosomal subunits . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition to viral proteins , several cellular proteins , such as heterogeneous nuclear ribonucleoprotein ( hnRNP ) A 1 , polypyrimidine tract binding ( PTB ) protein , poly ( A ) binding protein ( PABP ) , and mitochondrial aconitase ( m aconitase ) , have been identified to interact with the critical cis acting elements of coronavirus replication . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Yeast poly ( A ) binding protein , Pab 1 , and PAN , a poly ( A ) nuclease complex recruited by Pab 1 , connect mRNA biogenesis to export . ^^^ In this study , we identify the poly ( A ) binding protein , Pab 1 , and the poly ( A ) nuclease , PAN , as important factors that couple 3 ' processing to export . ^^^ Retention is also seen in cells lacking PAN , which Pab 1 is thought to recruit and which may be responsible for the final step of mRNA biogenesis , trimming of the poly ( A ) tail to the length found on newly exported mRNAs . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Although the 3 ' poly ( A ) tail of eukaryotic mRNAs and its major bound protein , the poly ( A ) binding protein ( PABP ) , have been studied extensively , their mechanism of action in translation is not well understood and is confounded by differences between in vivo and in vitro systems . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| However , the cytoplasmic somatic cell poly ( A ) binding protein ( PABP 1 ) is not expressed until later in embryogenesis . ^^^ We recently identified an embryonic poly ( A ) binding protein ( ePAB ) in Xenopus . ePAB is the predominant cytoplasmic PABP in Xenopus oocytes and early embryos and prevents deadenylation of mRNAs , suggesting its importance in the regulation of gene expression during early Xenopus development . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The functional relationship of ATX 2 and Pbp 1 is further corroborated by the experimental confirmation of the predicted interaction of ATX 2 with the cytoplasmic poly ( A ) binding protein 1 ( PABP ) using yeast 2 hybrid analysis as well as co immunoprecipitation experiments . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here , we identified two types of cDNA clones coding for poly ( A ) binding protein ( PABP ) and inducible PABP ( iPABP ) by screening an expression cDNA library with the GST Tob probe . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In a genetic screen for germ line proliferation defective mutants , we identified mutations in rpl 11 . 1 ( L 11 protein of the large ribosomal subunit ) , pab 1 [ a poly ( A ) binding protein ] , and glp 3 / eft 3 ( an elongation factor 1 alpha homolog ) . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The function of poly ( A ) binding protein 1 ( PABP 1 ) in poly ( A ) mediated translation has been extensively characterized . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| TcUBP 1 binds specific transcripts in vivo and co localizes in the perinuclear part of the cell with components of the mRNA stability determinant pathway such as poly ( A ) binding protein 1 ( PABP 1 ) and TcUBP 2 , a closely related RRM containing protein . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Yeast poly ( A ) binding protein Pab 1 shuttles between the nucleus and the cytoplasm and functions in mRNA export . ^^^ Pab 1 is the major poly ( A ) binding protein in yeast . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Previously , we found a regulatory element within the 3 ' untranslated region ( UTR ) of YB 1 mRNA that specifically interacted with YB 1 and poly ( A ) binding protein ( PABP ) ; we also showed that PABP positively affected YB 1 mRNA translation in a poly ( A ) tail independent manner ( O . 5 . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Both neuronal aldolases interact specifically with the NF L but not the heavy neurofilament mRNA , and their binding to the transcript excludes the poly ( A ) binding protein ( PABP ) from the complex . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Because poly ( A ) binding protein ( PABP ) is a dominant effector of poly ( A ) dependent translation we reasoned excess PABP in our extract might overwhelm PLE regulation of translation . ^^^ This was confirmed by experiments where PABP was inactivated with poly ( rA ) or Paip 2 , and the effect of both treatments was reversed by addition of recombinant PABP . ^^^ These data indicate that the PLE functionally substitutes for bound PABP to stimulate translation of short poly ( A ) mRNA . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein 1 ( PABP 1 ) is the RNA binding protein that attaches to the poly ( A ) tail of mRNA and is involved in translation and mRNA degradation . ^^^ METHODS : Using 12 muscle biopsies with sporadic IBM and 46 controls , the authors localized PABP 1 by immunohistochemistry , and poly ( A ) containing RNA ( poly ( A ) + RNA ) using the in situ hybridization method . ^^^ An immunofluorescence study indicated that large PABP 1 positive deposits formed conglomerates with poly ( A ) + RNA and PABP 1 colocalized with HuR . ^^^ Although PABP 1 positive cytoplasmic inclusions were found in disease controls , their aggregates combined with poly ( A ) + RNA were only detected in IBM . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The newly elongated poly ( A ) tail becomes bound by poly ( A ) binding protein ( PABP ) , which in turn binds eIF4G and helps it displace Maskin from eIF4E , thereby inducing translation . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Inhibitory effect of naked neural BC 1 RNA or BC 200 RNA on eukaryotic in vitro translation systems is reversed by poly ( A ) binding protein ( PABP ) . ^^^ Addition of recombinant human poly ( A ) binding protein ( PABP ) significantly compensated the inhibitory effect of the small oligo ( A ) rich RNA . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A FLAG tagged poly ( A ) binding protein ( PABP ) is expressed in a specific tissue and mRNA from that tissue is thus tagged by the recombinant PABP and separated from mRNA in other tissues by co immunoprecipitation with a FLAG tag specific antibody . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Reduced stability of mitogen activated protein kinase kinase 2 mRNA and phosphorylation of poly ( A ) binding protein ( PABP ) in cells overexpressing PABP . ^^^ The poly ( A ) binding protein ( PABP ) is an important regulator of mRNA translation and stability . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Repression of poly ( A ) binding protein ( PABP ) mRNA translation involves the binding of PABP to the adenine rich autoregulatory sequence ( ARS ) in the 5 ' untranslated region of its own mRNA . ^^^ There is also a direct relationship between the length of the poly ( A ) RNAs and their ability to form a trimeric complex with PABP , and to repress mRNA translation . ^^^ UV crosslinking studies suggest that the ARS is less efficient than a poly ( A ) RNA of similar length , to bind to PABP . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In immuno selection experiments using PKP 3 specific antibodies , we have identified by mass spectrometric analysis the following RNA binding proteins : Poly ( A ) binding protein ( PABPC 1 ) , fragile 10 related protein ( FXR 1 ) , and ras GAP SH 3 binding protein ( G3BP ) . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The PABC domain is a peptide binding domain that is specifically found in poly ( A ) binding protein ( PABP ) and a HECT ubiquitin protein isopeptide ligase ( E 3 ) known as HYD ( hyperplastic discs ) , EDD ( E 3 isolated by differential display ) , or Rat 100 . ^^^ The PABC domain of PABP recruits various regulatory proteins and translation factors to poly ( A ) mRNAs through binding of a conserved 12 amino acid peptide motif , PAM 2 ( PABP interacting motif 2 ) . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| AUF 1 is shown to directly interact with poly ( A ) binding protein ( PABP ) , both independently of eIF4G and in a complex with eIF4G . ^^^ Based on these and other data , we propose a model for the molecular interactions of AUF 1 that involves translation dependent displacement of AUF 1 PABP complexes from ARE mRNAs with possible unmasking of the poly ( A ) tail . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In this issue of the Biochemical Journal , Alvarez and colleagues have identified PABP 1 [ poly ( A ) binding protein 1 ] as a target of protease cleavage during HIV infection . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Poly ( A ) binding protein ( PABP ) homeostasis is mediated by the stability of its inhibitor , Paip 2 . ^^^ The poly ( A ) binding protein ( PABP ) is a unique translation initiation factor in that it binds to the mRNA 3 ' poly ( A ) tail and stimulates recruitment of the ribosome to the mRNA at the 5 ' end . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| The PABP [ poly ( A ) binding protein ] is able to interact with the 3 ' poly ( A ) tail of eukaryotic mRNA , promoting its translation . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| In the immunoprecipitation analysis , the poly ( A ) binding protein ( PABP ) and the RNA binding protein HuR were pulled down by 3 ' UTR cRNA , and the absence of the ARE site reduced the binding of these proteins . ^^^ Collectively , our results suggest that beta casein mRNA is protected from degradation by virtue of the structural interaction between the 3 ' UTR and poly ( A ) tail via a protein complex of HuR and PABP . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| A mechanism of translational repression by competition of Paip 2 with eIF4G for poly ( A ) binding protein ( PABP ) binding . ^^^ This interaction is mediated by the cap binding protein eIF4E , the poly ( A ) binding protein ( PABP ) , and eIF4G , a scaffolding protein that bridges between eIF4E and PABP to bring about the circularization of the mRNA . ^^^ The translational repressor , Paip 2 ( PABP interacting protein 2 ) , inhibits translation by promoting the dissociation of PABP from poly ( A ) . ^^^ Thus , Paip 2 can inhibit translation by a previously unrecognized mechanism , which is independent of its ability to disrupt PABP poly ( A ) interaction . . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We identified poly ( A ) binding protein 1 ( PABP ) as a novel BRCA 1 interacting protein in a yeast two hybrid screen and confirmed the interaction by in vitro assays and coimmunoprecipitation in mammalian cells . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| This synergy is mediated via interactions between eIF4G ( a component of the eIF4F cap binding complex ) and poly ( A ) binding protein ( PABP ) . ^^^ Paip2B acts in a manner similar to Paip2A to inhibit translation of capped and polyadenylated mRNAs both in vitro and in vivo by displacing PABP from the poly ( A ) tail . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have used detergent based cellular fractionation coupled with immunofluorescence microscopy to investigate the subcellular localisation in NIH3T3 fibroblasts of the initiation factors involved in recruitment of mRNA for translation , focussing on eIF4E , the mRNA cap binding protein , the scaffold protein eIF4GI and poly ( A ) binding protein ( PABP ) . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| It has been reported that the expression of the yeast poly ( A ) binding protein gene ( PAB 1 ) in plants leads to an induction of disease resistance responses , accompanied by alterations in the growth habit of the plant ( Li et al . ^^^ The regulation entailed the linking of the expression of the PAB 1 gene to control by the lac repressor , and by linking lac repressor expression to the disease resistance state of the plant , such that the induction of systemic defense responses by accumulation of the yeast poly ( A ) binding protein would turn off the expression of the PAB 1 gene . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here , we demonstrate that ataxin 2 and its Drosophila homolog , ATX 2 , assemble with polyribosomes and poly ( A ) binding protein ( PABP ) , a key regulator of mRNA translation . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| DAZ interacts with PABP [ poly ( A ) binding protein ] and thereby stimulates the translation of mRNAs containing short poly ( A ) tails [ Collier , Gorgoni , Loveridge , Cooke and Gray ( 2005 ) EMBO J . 24 , 2656 2666 ] . ^^^ |
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| Interacting proteins: Q15366 and P11940 |
Pubmed |
SVM Score :0.0 |
| For the initiation of translation of most cellular mRNAs , the cap structure at the 5 ' end is bound by the translation factor eukaryotic initiation factor 4E ( eIF4E ) , while the poly ( A ) tail , at the 3 ' end , is recognized by the poly ( A ) binding protein ( PABP ) . eIF4G is a scaffold protein that brings together eIF4E and PABP , causing the circularization of the mRNA that is thought to be important for an efficient initiation of translation . ^^^ Rotavirus mRNAs lack a poly ( A ) tail but have instead a consensus sequence at their 3 ' ends that is bound by the viral nonstructural protein NSP 3 , which also interacts with eIF4GI , using the same region employed by PABP . ^^^ |
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