| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :1.1546111 |
| Together , phosphorylated and hypophosphorylated PABP exhibited synergistic binding . eIF4B interacted with PABP in a phosphorylation state specific manner ; native eIF4B increased the RNA binding activity specifically of phosphorylated PABP and was greater than 14 fold more effective than was recombinant eIF4B , whereas eIF4F promoted the cooperative binding of hypophosphorylated PABP . 1.1546111^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.67862774 |
| In yeast and plants , PABP interacts with eIF4G but no such interaction has been reported in mammalian cells . 0.67862774^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.53656926 |
| We now show that cellular stresses such as arsenite , anisomycin and heat shock also result in increased phosphorylation of eIF4E , eIF4F complex formation and the association of PABP with eIF4G , in conditions under which the rate of protein synthesis is severely inhibited . 0.53656926^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.50849646 |
| In contrast , Paip 2 strongly inhibits translation by promoting dissociation of PABP from poly ( A ) and by competing with eIF4G for binding to PABP . . 0.50849646^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Mutations in CDC 33 , CEG 1 , PRT 1 , PAB 1 , and TIF 4631 , encoding eIF4G1 , have been shown to lead to destabilization of mRNAs . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) interacts with the cap associated eukaryotic initiation factor ( eIF ) 4G ( in yeast and plants ) and eIF4B ( in plants ) , a functional consequence of which is to increase the affinity of PABP for poly ( A ) and to increase the affinity of the cap binding complex , eIF4F ( of which eIF4G is a subunit ) for the 5 ' cap structure . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that the amount of poly ( A ) binding protein ( PABP ) present in eIF4F complexes decreases during rotavirus infection , even though eIF4A and eIF4E remain unaffected . ^^^ PABP is removed from the eIF4F complex after incubation in vitro with the C terminal part of NSP3A , but not with its N terminal part produced in E . coli . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate the serum stimulated co isolation of the poly ( A ) binding protein ( PABP ) with the eIF4F complex . ^^^ This apparent interaction of PABP with eIF4F suggests that a mechanism shown to be important in the control of translation in the yeast Saccharomyces cerevisiae also operates in vertebrate cells . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| These results suggest that , in terms of the scanning model of translation initiation , PABP may enhance the mRNA scanning rate of the complex formed by eIF4A , eIF4B , and eIF4F or eIF ( iso ) 4F and increase the rate of translation . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The eukaryotic initiation factor complex eIF4F , which in yeast contains the core components eIF4E and eIF4G , uses the cap as a binding site , serving as an initial point of assembly for the translation apparatus , and also binds the poly ( A ) binding protein Pab 1 . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The circularisation model of the polysome suggests that ribosome recycling is facilitated by 5 ' 3 ' interactions mediated by the cap binding complex eIF4F and the poly ( A ) binding protein , Pab 1 . ^^^ Changes in termination kinetics or disruption of the Pab 1 eIF4F interaction do not affect recycling , yet the maintenance of normal in vivo mRNP structure is important to this process . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| This synergy is mediated via interactions between eIF4G ( a component of the eIF4F cap binding complex ) and poly ( A ) binding protein ( PABP ) . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The cleavage of PABP correlated better over time with the host translational shutoff and onset of viral protein synthesis than did the cleavage of eIF4G . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| In yeast and plants , binding of eIF4G to poly ( A ) binding protein ( PABP ) was implicated in poly ( A ) dependent translation . ^^^ In mammals , however , there has been no evidence that eIF4G binds PABP . ^^^ As previously shown for yeast , human eIF4G binds to a fragment composed of RRM 1 and RRM 2 of PABP . ^^^ These results indicate that , in addition to a recently identified mammalian PABP binding protein , PAIP 1 , eIF4G binds PABP and probably functions in poly ( A ) dependent translation in mammalian cells . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Most eukaryotic mRNAs possess a 5 ' cap structure ( m ( 7 ) GpppN ) and a 3 ' poly ( A ) tail which promote translation initiation by binding the eukaryotic translation initiation factor ( eIF ) 4E and the poly ( A ) binding protein ( PABP ) , respectively . eIF4G can bridge between eIF4E and PABP , and through eIF 3 is thought to establish a link to the small ribosomal subunit . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The 5 ' cap and poly ( A ) tail of eukaryotic mRNAs work synergistically to enhance translation through a process that requires interaction of the cap associated eukaryotic initiation factor , eIF 4G , and the poly ( A ) binding protein , PABP . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Moreover , we show that the effect of binding of full length eIF4G on the cap affinity of eIF4E can be further modulated through binding of Pab 1 to eIF4G . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Maximal cap poly ( A ) cooperativity required the integrity of the eukaryotic initiation factor 4G poly ( A ) binding protein ( eIF4G PABP ) interaction , suggesting that synergy results from mRNA circularization . ^^^ These effects of poly ( A ) were also sensitive to disruption of the eIF4G PABP interaction , suggesting that 5 ' 3 ' end cross talk is functionally conserved between classical mRNAs and an IRES containing mRNA . ^^^ Finally , we demonstrate that a rotaviral non structural protein that evicts PABP from eIF4G is capable of provoking the shut off of host cell translation seen during rotavirus infection . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Although the eIF4G PABP interaction has also been demonstrated in a mammalian system [ 3 , 4 ] , its biological significance in vertebrates is unknown . ^^^ Because the amount of PABP is very low in oocytes [ 8 ] , it has been argued that the eIF4G PABP interaction does not play a major role in translational activation during oocyte maturation . ^^^ Our results show that the eIF4G PABP interaction is critical for translational control of maternal mRNAs during Xenopus development . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| This effect is mediated by a bridging complex , composed of the poly ( A ) binding protein ( PABP ) , eIF4G , and the cap binding protein , eIF4E . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| This translational effect of Ssa appears mediated , at least in part , by its affect on the interaction of Pab 1 with the translation initiation factor , eIF4G , which is dramatically reduced in the absence of functional Ssa protein . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| In this model , the transcript termini are brought together by interactions of poly ( A ) binding protein ( PABP ) with both the poly ( A ) tail and initiation factor eIF4G . ^^^ We considered the possibility that the cytosolic inhibitor blocked the interaction of PABP with the poly ( A ) tail or with eIF4G . ^^^ We found that neither of these interactions were inhibited , as shown by immunoprecipitation of PABP followed by quantitation of the poly ( A ) tail by reverse transcription PCR and of eIF4G by immunoblot analysis . ^^^ These data show that translational silencing of Cp requires interactions of three essential elements of mRNA circularization , poly ( A ) , PABP , and eIF4G . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The interaction between eukaryotic translation initiation factor 4G ( eIF4G ) and the poly ( A ) binding protein ( PABP ) facilitates translational initiation of polyadenylated mRNAs . ^^^ These results strongly suggest that the eIF4G PABP interaction plays a critical role in the translational control of maternal mRNAs during oocyte maturation . ^^^ In the present work , we employed another strategy to interfere eIF4G PABP interaction in Xenopus oocytes . ^^^ The amino terminal part of eIF4GI containing the PABP binding site ( 4GNt M 1 ) was expressed in Xenopus oocytes . 4GNt M 1 could bind to PABP in oocytes , which suggests that 4GNt M 1 may evict PABP from the endogenous eIF4G . ^^^ These results further support the idea that the eIF4G PABP interaction is critical for translational regulation of maternal mRNAs in oocytes . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| EIF4G interacts with several other factors including eIF4E , which is a cap binding protein , and the poly ( A ) binding protein ( PABP ) . ^^^ In this work , we demonstrate that the expression of the amino terminal one third of eIF4G , which interacts with eIF4E and PABP , in Xenopus oocyte inhibits translation and progesterone induced maturation . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Site directed mutagenesis and isothermal titration calorimetry documented that NSP 3 and PABP use analogous eIF4G recognition strategies , despite marked differences in tertiary structure . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| There was a PABP dependent linkage between the termination factor complex eRF 1 GSPT and the initiation factor eIF4G associating with 5 ' cap through eIF4E . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| PABP is composed of two parts : the highly conserved N terminus , containing 4 RNA recognition motifs ( RRMs ) responsible for poly ( A ) and eIF4G binding ; and the more variable C terminus , which includes the recently described PABC domain , and promotes intermolecular interaction between PABP molecules as well as cooperative binding to poly ( A ) . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| We propose that enteroviruses use a dual strategy for host translation shutoff , requiring cleavage of PABP by 3Cpro and of eIF4G by 2Apro . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| This phenomenon has been explained by the simultaneous binding of poly ( A ) binding protein ( PABP ) and a cap binding protein ( eIF4E ) to eIF4G that results in the circularization of the mRNA ( closed loop model ) . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| We show that the viral 100k protein possesses a selective binding element for the 5 ' noncoding region ( 5 ' NCR ) of viral late mRNAs ( known as the tripartite leader ) , forms a complex with initiation factor eIF4G and poly ( A ) binding protein ( PABP ) , and strongly and selectively enhances the level of both factors and 40S ribosome subunits on viral mRNAs in polysomes . ^^^ A molecular mechanism for ribosome shunting is described by which enhanced binding of eIF4G and possibly PABP with 100k protein , and simultaneous interaction with the tripartite leader 5 ' NCR , drives 40S ribosome recruitment and initiation on mRNAs . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Enhancement of IRES mediated translation of the c myc and BiP mRNAs by the poly ( A ) tail is independent of intact eIF4G and PABP . ^^^ The PABP eIF4G bridging complex that is critical for cap dependent translation is thus dispensable for the enhancement of the IRESs by the poly ( A ) tail . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| These studies show an association of ICP 27 with the cellular translation initiation factors poly A binding protein ( PABP ) , eukaryotic initiation factor 3 ( eIF 3 ) , and eukaryotic initiation factor 4G ( eIF4G ) in infected cells . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Supplementing PABP depleted extracts with wild type PABP completely rectified these deficiencies , whereas a mutant of PABP , M161A , which is incapable of interacting with eIF4G , failed to restore translation . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| In contrast , eIF 3 , G3BP , eIF4G , and PABP 1 are restricted to SGs , whereas DCP1a and 2 are confined to PBs . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The newly elongated poly ( A ) tail becomes bound by poly ( A ) binding protein ( PABP ) , which in turn binds eIF4G and helps it displace Maskin from eIF4E , thereby inducing translation . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| In contrast we find that nuclear cap binding proteins ( CBP 20 and 80 ) , eIF4G , and PAB 1 are absolutely required for development . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| CBV infection resulted in efficient cleavage of eIF4G and PABP , coincident with polyribosome breakdown in the cytosol and ER compartments . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| AUF 1 is shown to directly interact with poly ( A ) binding protein ( PABP ) , both independently of eIF4G and in a complex with eIF4G . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| A mechanism of translational repression by competition of Paip 2 with eIF4G for poly ( A ) binding protein ( PABP ) binding . ^^^ This interaction is mediated by the cap binding protein eIF4E , the poly ( A ) binding protein ( PABP ) , and eIF4G , a scaffolding protein that bridges between eIF4E and PABP to bring about the circularization of the mRNA . ^^^ Here we report on the existence of an alternative mechanism by which Paip 2 inhibits translation by competing with eIF4G for binding to PABP . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| For the initiation of translation of most cellular mRNAs , the cap structure at the 5 ' end is bound by the translation factor eukaryotic initiation factor 4E ( eIF4E ) , while the poly ( A ) tail , at the 3 ' end , is recognized by the poly ( A ) binding protein ( PABP ) . eIF4G is a scaffold protein that brings together eIF4E and PABP , causing the circularization of the mRNA that is thought to be important for an efficient initiation of translation . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The mammalian eukaryotic initiation factor 4GI ( eIF4GI ) may be divided into three roughly equal regions ; an amino terminal one third ( amino acids [ aa ] 1 to 634 ) , which contains the poly ( A ) binding protein ( PABP ) and eIF4E binding sites ; a middle third ( aa 635 to 1039 ) , which binds eIF4A and eIF 3 ; and a carboxy terminal third ( aa 1040 to 1560 ) , which harbors a second eIF4A binding site and a docking sequence for the Ser / Thr kinase Mnk 1 . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition to cleavage of eIF4GI , cleavage of eIF4GII and poly ( A ) binding protein ( PABP ) has been recently proposed to contribute to complete host translation shutoff ; however , the relative importance of eIF4GII and PABP cleavage has not been determined . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| PABP 1 and eIF4GI associate with influenza virus NS 1 protein in viral mRNA translation initiation complexes . ^^^ We have previously reported that NS 1 interacts with the translation initiation factor eIF4GI , next to its poly ( A ) binding protein 1 ( PABP 1 ) interacting domain and that NS 1 and eIF4GI are associated in influenza virus infected cells . ^^^ Here we show that NS 1 , although capable of binding poly ( A ) , does not compete with PABP 1 for association with eIF4GI and , furthermore , that NS 1 and PABP 1 interact both in vivo and in vitro in an RNA independent manner . ^^^ These mapping studies , together with those previously reported for NS 1 eIF4GI and PABP 1 eIF4GI interactions , imply that the binding of all three proteins would be compatible . ^^^ Collectively , these and previously published data suggest that NS 1 interactions with eIF4GI and PABP 1 , as well as with viral mRNAs , could promote the specific recruitment of 43S complexes to the viral mRNAs . . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| The degradation of PABP correlated with translation inhibition but lagged behind cleavage of eIF4GI . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Therefore , at least in this way , the pioneer round of translation is distinct from steady state translation . eIF4GI , poly ( A ) binding protein ( PABP ) 1 , eIF 3 , eIF4AI , and eIF2alpha coimmunopurify with both CBP 80 and eIF4E , which suggests that each factor functions in both modes of translation . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| Furthermore , eIF4GI is localised primarily at the nuclear periphery in the vicinity of eIF4E and PABP 1 . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have used detergent based cellular fractionation coupled with immunofluorescence microscopy to investigate the subcellular localisation in NIH3T3 fibroblasts of the initiation factors involved in recruitment of mRNA for translation , focussing on eIF4E , the mRNA cap binding protein , the scaffold protein eIF4GI and poly ( A ) binding protein ( PABP ) . ^^^ |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q04637 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|