| Interacting proteins: P23588 and P11940 |
Pubmed |
SVM Score :0.80420472 |
| The interactions between PABP and eIF 4F , eIF iso4F , and eIF 4B were measured in the absence of poly ( A ) RNA using far Western analysis and confirmed by direct fluorescence titration studies . 0.80420472^^^ |
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| Interacting proteins: P23588 and P11940 |
Pubmed |
SVM Score :1.1456653 |
| Together , phosphorylated and hypophosphorylated PABP exhibited synergistic binding . eIF4B interacted with PABP in a phosphorylation state specific manner ; native eIF4B increased the RNA binding activity specifically of phosphorylated PABP and was greater than 14 fold more effective than was recombinant eIF4B , whereas eIF4F promoted the cooperative binding of hypophosphorylated PABP . 1.1456653^^^ |
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| Interacting proteins: P23588 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) interacts with the cap associated eukaryotic initiation factor ( eIF ) 4G ( in yeast and plants ) and eIF4B ( in plants ) , a functional consequence of which is to increase the affinity of PABP for poly ( A ) and to increase the affinity of the cap binding complex , eIF4F ( of which eIF4G is a subunit ) for the 5 ' cap structure . ^^^ |
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| Interacting proteins: P23588 and P11940 |
Pubmed |
SVM Score :0.0 |
| Recent studies demonstrated that wheat germ poly ( A ) binding protein ( PABP ) interacted with translation eukaryotic initiation factor ( eIF ) iso4G and eIF4B , and these interactions increased the poly ( A ) binding activity of PABP ( Le , H . , Tanguay , R . ^^^ We report here that the interaction between PABP and eIF iso4G has a substantial effect on the ATPase activity and RNA helicase activity of ( eIF4A + eIF4B + eIF iso4F ) complex . ^^^ These results suggest that , in terms of the scanning model of translation initiation , PABP may enhance the mRNA scanning rate of the complex formed by eIF4A , eIF4B , and eIF4F or eIF ( iso ) 4F and increase the rate of translation . . ^^^ |
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| Interacting proteins: P23588 and P11940 |
Pubmed |
SVM Score :0.0 |
| The kinetic effects of eIF4B , PABP , and wheat germ eIFiso4F with two mRNA cap analogues and the temperature dependence of this reaction were measured and compared . ^^^ Ant m ( 7 ) GTP and eIFiso4F . m ( 7 ) GpppG , respectively , in the presence of eIF4B and PABP . ^^^ These studies show that eIF4B and PABP enhance the interaction with the cap and probably are involved in protein protein interactions as well . ^^^ The temperature dependence of the cap binding reaction was markedly reduced in the presence of either eIF4B or PABP . ^^^ However , when both eIF4B and PABP were present , not only was the energy barrier reduced but the binding rate was faster . ^^^ |
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