| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :1.1546111 |
| Together , phosphorylated and hypophosphorylated PABP exhibited synergistic binding . eIF4B interacted with PABP in a phosphorylation state specific manner ; native eIF4B increased the RNA binding activity specifically of phosphorylated PABP and was greater than 14 fold more effective than was recombinant eIF4B , whereas eIF4F promoted the cooperative binding of hypophosphorylated PABP . 1.1546111^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that the amount of poly ( A ) binding protein ( PABP ) present in eIF4F complexes decreases during rotavirus infection , even though eIF4A and eIF4E remain unaffected . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Although rapamycin abrogated serum enhanced rates of protein synthesis and the interaction of eIF4G with eIF4E , it did not prevent the increase in association of eIF4G with PABP . ^^^ This suggests that serum stimulates the interaction between eIF4G and PABP by a distinct mechanism that is independent of both the mTOR pathway and the enhanced association of eIF4G with eIF4E . . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Most eukaryotic mRNAs possess a 5 ' cap structure ( m ( 7 ) GpppN ) and a 3 ' poly ( A ) tail which promote translation initiation by binding the eukaryotic translation initiation factor ( eIF ) 4E and the poly ( A ) binding protein ( PABP ) , respectively . eIF4G can bridge between eIF4E and PABP , and through eIF 3 is thought to establish a link to the small ribosomal subunit . ^^^ We fused the C terminal region of human eIF4GI lacking both the eIF4E and PABP binding sites , to the IRE binding protein IRP 1 . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have shown previously that the stimulation of cultured Xenopus kidney cells with serum resulted in the activation of protein synthesis , enhanced phosphorylation of eIF4E and increased binding of the adapter protein , eIF4G , and poly ( A ) binding protein ( PABP ) to eIF4E to form the functional initiation factor complex , eIF4F / PABP . ^^^ We now show that cellular stresses such as arsenite , anisomycin and heat shock also result in increased phosphorylation of eIF4E , eIF4F complex formation and the association of PABP with eIF4G , in conditions under which the rate of protein synthesis is severely inhibited . ^^^ These results indicate that cellular stresses activate multiple signalling pathways that converge at the level of eIF4F complex formation to influence the interactions between eIF4E , eIF4G and PABP . . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| The mammalian eukaryotic initiation factor 4GI ( eIF4GI ) may be divided into three roughly equal regions ; an amino terminal one third ( amino acids [ aa ] 1 to 634 ) , which contains the poly ( A ) binding protein ( PABP ) and eIF4E binding sites ; a middle third ( aa 635 to 1039 ) , which binds eIF4A and eIF 3 ; and a carboxy terminal third ( aa 1040 to 1560 ) , which harbors a second eIF4A binding site and a docking sequence for the Ser / Thr kinase Mnk 1 . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Moreover , we show that the effect of binding of full length eIF4G on the cap affinity of eIF4E can be further modulated through binding of Pab 1 to eIF4G . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| The eukaryotic initiation factor complex eIF4F , which in yeast contains the core components eIF4E and eIF4G , uses the cap as a binding site , serving as an initial point of assembly for the translation apparatus , and also binds the poly ( A ) binding protein Pab 1 . ^^^ We show that Dcp 1 binds to eIF4G and Pab 1 as free proteins , as well as to the complex eIF4E eIF4G Pab 1 . ^^^ Dcp 1 interacts with the N terminal region of eIF4G but does not compete significantly with eIF4E or Pab 1 for binding to eIF4G . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Biochemical characterisation of cap poly ( A ) synergy in rabbit reticulocyte lysates : the eIF4G PABP interaction increases the functional affinity of eIF4E for the capped mRNA 5 ' end . ^^^ Additionally , we show that the eIF4G PABP interaction on mRNAs which are capped and polyadenylated significantly increases the affinity of eIF4E for the 5 ' cap . . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| We identified mRNA subsets from P 19 embryonal carcinoma stem cells by using mRNA binding proteins HuB , eIF 4E , and PABP that are known to play a role in translation . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| This effect is mediated by a bridging complex , composed of the poly ( A ) binding protein ( PABP ) , eIF4G , and the cap binding protein , eIF4E . ^^^ |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| EIF4G interacts with several other factors including eIF4E , which is a cap binding protein , and the poly ( A ) binding protein ( PABP ) . ^^^ In this work , we demonstrate that the expression of the amino terminal one third of eIF4G , which interacts with eIF4E and PABP , in Xenopus oocyte inhibits translation and progesterone induced maturation . . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| There was a PABP dependent linkage between the termination factor complex eRF 1 GSPT and the initiation factor eIF4G associating with 5 ' cap through eIF4E . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| This phenomenon has been explained by the simultaneous binding of poly ( A ) binding protein ( PABP ) and a cap binding protein ( eIF4E ) to eIF4G that results in the circularization of the mRNA ( closed loop model ) . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Therefore , at least in this way , the pioneer round of translation is distinct from steady state translation . eIF4GI , poly ( A ) binding protein ( PABP ) 1 , eIF 3 , eIF4AI , and eIF2alpha coimmunopurify with both CBP 80 and eIF4E , which suggests that each factor functions in both modes of translation . ^^^ |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Furthermore , eIF4GI is localised primarily at the nuclear periphery in the vicinity of eIF4E and PABP 1 . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In order to approach these questions , we have used RNA binding proteins such as ELAV / Hu , poly ( A ) binding protein ( PABP ) and cap binding protein ( eIF 4E ) as reporters of gene expression . ^^^ |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| Using a new technique to deplete PABP from mammalian cell extracts , we show that extracts lacking PABP exhibit dramatically reduced rates of translation , reduced efficiency of 48S and 80S ribosome initiation complex formation , and impaired interaction of eIF4E with the mRNA cap structure . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| The newly elongated poly ( A ) tail becomes bound by poly ( A ) binding protein ( PABP ) , which in turn binds eIF4G and helps it displace Maskin from eIF4E , thereby inducing translation . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| In these cells , the levels of phosphorylated PABP , eIF4E , and ERK 2 are also reduced . ^^^ Thus , a novel signaling pathway involving MKK 2 and ERK1 / 2 may down regulate the activity of PABP and eIF4E by controlling their phosphorylation and compensates for the effect of excess cellular PABP . . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| This interaction is mediated by the cap binding protein eIF4E , the poly ( A ) binding protein ( PABP ) , and eIF4G , a scaffolding protein that bridges between eIF4E and PABP to bring about the circularization of the mRNA . ^^^ |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| We have used detergent based cellular fractionation coupled with immunofluorescence microscopy to investigate the subcellular localisation in NIH3T3 fibroblasts of the initiation factors involved in recruitment of mRNA for translation , focussing on eIF4E , the mRNA cap binding protein , the scaffold protein eIF4GI and poly ( A ) binding protein ( PABP ) . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| This interaction is mediated by a ribonucleoprotein network that contains , at a minimum , the poly ( A ) binding protein ( PABP ) , the capbinding protein eIF4E and a scaffolding protein , eIF4G . eIF4G , in turn , contains binding sites for eIF4A and eIF 3 , a 40S ribosome associated initiation factor . ^^^ The PABP poly ( A ) interaction also stimulates initiation driven by picomavirus ' internal ribosomal entry sites ( IRESs ) , a process that requires eIF4G but not eIF4E . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| For the initiation of translation of most cellular mRNAs , the cap structure at the 5 ' end is bound by the translation factor eukaryotic initiation factor 4E ( eIF4E ) , while the poly ( A ) tail , at the 3 ' end , is recognized by the poly ( A ) binding protein ( PABP ) . eIF4G is a scaffold protein that brings together eIF4E and PABP , causing the circularization of the mRNA that is thought to be important for an efficient initiation of translation . ^^^ |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| The poly ( A ) binding protein ( PABP ) interacts with the cap associated eukaryotic initiation factor ( eIF ) 4G ( in yeast and plants ) and eIF4B ( in plants ) , a functional consequence of which is to increase the affinity of PABP for poly ( A ) and to increase the affinity of the cap binding complex , eIF4F ( of which eIF4G is a subunit ) for the 5 ' cap structure . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| These results suggest that , in terms of the scanning model of translation initiation , PABP may enhance the mRNA scanning rate of the complex formed by eIF4A , eIF4B , and eIF4F or eIF ( iso ) 4F and increase the rate of translation . . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| The circularisation model of the polysome suggests that ribosome recycling is facilitated by 5 ' 3 ' interactions mediated by the cap binding complex eIF4F and the poly ( A ) binding protein , Pab 1 . ^^^ Changes in termination kinetics or disruption of the Pab 1 eIF4F interaction do not affect recycling , yet the maintenance of normal in vivo mRNP structure is important to this process . ^^^ |
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| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| This synergy is mediated via interactions between eIF4G ( a component of the eIF4F cap binding complex ) and poly ( A ) binding protein ( PABP ) . ^^^ |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06730 and P11940 |
Pubmed |
SVM Score :0.0 |
| NA |
|