| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.50301766 |
| Anti alpha 1 syntrophin antibodies gave strong labeling of the sarcolemma and NMJ in normal rat and mouse muscle , and similar but much weaker labeling in dystrophin minus mdx muscle . beta 2 Syntrophin therefore appears to be specific to the NMJ in normal muscle , as is utrophin , and may be involved in acetylcholine receptor clustering . alpha 1 Syntrophin appears to be associated mainly with dystrophin , as expected , but a small portion must be associated with another protein , possibly homologs of the electric tissue 87K protein . . 0.50301766^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.95682951 |
| Finally , we used in vitro translated proteins in an immunoprecipitation assay to show that , like beta 1 syntrophin , both beta 2 and alpha 1 syntrophin interact with peptides encoding the syntrophin binding region of dystrophin , utrophin / dystrophin related protein , and the Torpedo 87K protein . . 0.95682951^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :1.2469276 |
| The carboxy terminal region of dystrophin has previously been shown to interact directly with alpha 1 syntrophin , a cytoplasmic component of the dystrophin glycoprotein complex , by in vitro biochemical studies such as overlay assay or immunoprecipitation . 1.2469276^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| An NH 2 terminal domain of nNOS directly interacts with alpha 1 syntrophin but not with other proteins in the dystrophin complex analyzed . ^^^ However , nNOS does not associate with alpha 1 syntrophin on the sarcolemma in transgenic mdx mice expressing truncated dystrophin proteins . ^^^ This suggests a ternary interaction of nNOS , alpha 1 syntrophin , and the central domain of dystrophin in vivo , a conclusion supported by developmental studies in muscle . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Similarly , in dystrophin deficient skeletal muscles from mdx mice both soluble and particulate nNOS was greatly reduced compared with C 57 control mice . nNOS mRNA was also reduced in mdx muscle in contrast to mRNA levels for a dystrophin binding protein , alpha 1 syntrophin . nNOS levels increased dramatically from 2 to 52 weeks of age in C 57 skeletal muscle , which may indicate a physiological role for NO in aging related processes . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Mouse alpha 1 syntrophin binds the COOH terminal domain of dystrophin , and calmodulin inhibits this interaction in a Ca2+ dependent fashion . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| The results can be interpreted as indicating that , in general , NOS 1 targeting to the sarcolemma is dependent on particular members of the dystrophin complex , such as alpha 1 syntrophin , yet the expression and / or positioning of NOS 1 may be under the control of further factors , probably of neurogenic origin . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| In the hippocampal formation there is distinctive co localization of specific syntrophins with certain dystrophin isoforms in neurons , e . g . alpha 1 syntrophin with the C dystrophin in CA regions and beta 2 syntrophin with the G dystrophin in the dentate gyrus . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Neuronal nitric oxide synthase ( nNOS ) has been shown previously to interact with alpha 1 syntrophin in the dystrophin complex of skeletal muscle . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Ultrastructural localization of alpha 1 syntrophin and neuronal nitric oxide synthase in normal skeletal myofiber , and their relation to each other and to dystrophin . ^^^ We investigated the ultrastructural localization of alpha 1 syntrophin and neuronal nitric oxide synthase ( nNOS ) in normal human skeletal myofibers and analyzed their relation to each other and to dystrophin using single and double immunogold labeling electron microscopy . ^^^ Double immunolabeling revealed that epitope combinations of alpha 1 syntrophin dystrophin , alpha 1 syntrophin nNOS , and nNOS dystrophin occurred more frequently in doublet form than did other epitope combinations , such as alpha 1 syntrophin beta spectrin and nNOS beta spectrin . ^^^ A significantly higher percentage of doublets comprised antibodies against alpha 1 syntrophin and dystrophin ( 28 . 5 + / 1 . 5 % , group mean + / SE ) than those against alpha 1 syntrophin and beta spectrin ( 9 . 2 + / 0 . 8 % , P < 0 . 01 ) . ^^^ Furthermore , nNOS formed doublets significantly more frequently with dystrophin ( 25 . 2 + / 3 . 3 % ) and alpha 1 syntrophin ( 26 . 0 + / 4 . 1 % ) than with beta spectrin ( 13 . 9 + / 2 . 3 % ; P < 0 . 05 ) . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| These observations suggest that alpha 1 syntrophin may work as a modular adaptor protein that can link nNOS or other signaling enzyme to the sarcolemmal dystrophin complex . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Mller glial cells from rat retina express dystrophin protein Dp 71 , utrophin and the members of the dystrophin associated glycoprotein complex ( DGC ) , namely beta dystroglycan , delta and gamma sarcoglycans and alpha 1 syntrophin . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| The expression of dystrophin and alpha 1 syntrophin during skeletal muscle regeneration . ^^^ The expression of dystrophin and alpha 1 syntrophin in rat tibialis anterior muscles were evaluated during a cycle of regeneration after myonecrosis induced by the injection of cardiotoxin . ^^^ In Western blot analysis , dystrophin ( DYS 1 ) and alpha 1 syntrophin ( alpha1S ) were completely absent on day 1 . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Association of neuronal nitric oxide synthase ( nNOS ) with alpha 1 syntrophin at the sarcolemma . alpha 1 syntrophin is a PDZ containing dystrophin associated protein , expressed predominantly in striated muscle and brain . alpha 1 syntrophin null mice generated by gene targeting technique showed no overt muscular dystrophic phenotype . ^^^ In addition , the mice would facilitate identification of other signaling molecules , which are targeted to dystrophin complex via interaction with alpha 1 syntrophin . . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| Moreover , by double immunostaining we showed that beta dystroglycan co localizes with dystrophin in the outer plexiform layer whereas alpha 1 syntrophin labeling differs from that for dystrophins . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| This study investigated immunohistochemically the expression of alpha 1 syntrophin in Duchenne and Fukuyama muscular dystrophies ( DMD and FCMD , respectively ) . ^^^ Immunoblot analysis showed that anti alpha 1 syntrophin antibody had a decreased reaction in both DMD and FCMD muscle extracts . ^^^ The group mean percentages of alpha 1 syntrophin and beta spectrin immunonegative myofibers in the DMD group were 39 . 3 % and 10 . 8 % , respectively , while those in the FCMD group were 45 . 5 % and 10 . 4 % , respectively . ^^^ Thus we found that dystrophin deficient DMD muscles contained significant numbers of alpha 1 syntrophin positive fibers and significant numbers of alpha 1 syntrophin negative fibers were present in dystrophin positive muscles of severe muscular dystrophy such as FCMD . ^^^ Alpha 1 syntrophin , a scaffolding adapter and modular protein , is a cytoplasmic component of the dystrophin glycoprotein complex . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| These patterns of expression suggest that syntrophin 1 and syntrophin 2 may associate with different members of the dystrophin family . . ^^^ |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13424 and P11532 |
Pubmed |
SVM Score :0.0 |
| NA |
|