Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.62103573
Cultured fibroblasts from HS showed a strong nuclear staining of bFGF , but not from non HS and normal skin . bFGF R was equally expressed with a diffuse cytoplasmic pattern by fibroblasts from all sources . bFGF may play an important role in the pathological fibrotic process of HS in which fibroblasts are persistently activated . 0.62103573^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.60549373
Nuclear FGFR 1 binds to FGF 2 and has tyrosine kinase activity . 0.60549373^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :1.3203629
Wild type FGFR 1 , FGFR 1 3F , and FGFR 1 4F similarly bind to a 90 kDa tyrosine phosphorylated protein and activate Shc , extracellular signal regulated kinase ( ERK ) 2 , and JunD after stimulation with FGF 2 . 1.3203629^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.62673804
FGF 7 recognizes one FGFR isoform known as the FGFR 2 IIIb isoform or keratinocyte growth factor receptor ( KGFR ) , whereas FGF 2 binds well to FGFR 1 , FGFR 2 , and FGFR 4 but interacts poorly with KGFR . 0.62673804^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.5148221
Colocalisation of FGFR 1 with FGF 2 could not be demonstrated in unstressed retina , but was demonstrable in stressed retina , in both the ONL and OPL . 0.5148221^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have screened several cell lines derived from glioblastomas for anomalies in the expression of genes encoding transforming growth factor alpha ( TGF alpha ) , TGF beta , basic fibroblast growth factor ( bFGF ) and its high affinity receptor , flg . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Expression of basic fibroblast growth factor , FGFR 1 and FGFR 2 in normal and malignant human breast , and comparison with other normal tissues . ^^^ The expression of basic fibroblast growth factor ( bFGF ) and two of its receptors , FGFR 1 and FGFR 2 , was detected using the polymerase chain reaction , and quantified by comparison to the relative amount of product obtained following co amplification of the ubiquitous glyceraldehyde phosphate dehydrogenase transcript . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
A time course for the focal elevation of synthesis of basic fibroblast growth factor and one of its high affinity receptors ( flg ) following a localized cortical brain injury . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Antisense oligodeoxynucleotides targeted against the translation start site and a splice donor acceptor site of human FGFR 1 , in addition to inhibiting the proliferation of normal human melanocytes and malignant melanomas , caused extensive dendrite formation and severe disruption of cell cell contact morphological changes that were not observed upon inhibition of the genes encoding basic fibroblast growth factor ( bFGF ) and retinoic acid alpha receptor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
By using this technique we have isolated a clone encoding the murine basic fibroblast growth factor receptor ( bFGF R ) , as well as a shorter form of this mRNA . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To increase our understanding of the potential role of basic fibroblast growth factor ( bFGF ) in malignant progression of prostate cancer , we determined the production of bFGF , the expression of FGF receptor ( flg ) , and the response to exogenous bFGF in LNCaP , DU 145 , and PC 3 cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The amino acid sequence of the TK 14 protein is closely related to the available partial sequence of the mouse protein bek , and more distantly related to the sequences of a chicken basic fibroblast growth factor receptor ( 73 % sequence homology ) and the apparent human equivalent of this receptor , the FLG protein ( encoded by the fms like tyrosine kinase gene ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The K sam gene had significant homologies with bek , FLG , and chicken basic fibroblast growth factor receptor gene . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study , the expression of a acidic fibroblast growth factor ( aFGF ) , basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 were analyzed in 60 pancreatic cancer samples using Northern blot analysis , immunoblotting , in situ hybridization and immunohistochemical techniques . aFGF , bFGF and FGFR 1 were present in 63 % , 55 % and 52 % of the tumor samples , respectively . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( bFGF ) and two of its receptors , FGFR 1 and FGFR 2 : gene expression in the rat brain during postnatal development as determined by quantitative RT PCR . ^^^ Regional and temporal patterns of the expression of basic fibroblast growth factor ( bFGF ) , and two of its high affinity receptors ( FGFR 1 and FGFR 2 ) , were examined in the male rat brain during early postnatal development ; the reverse transcription polymerase chain reaction ( RT PCR ) was used to obtain mRNA measurements which were expressed relative to mRNA for GAPDH as a constant . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunohistochemistry and RT PCR of basic fibroblast growth factor ( bFGF ) and one of its receptors ( FGFR 1 ) were performed in pituitary adenomas . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( bFGF ) and its high affinity receptor FGFR 1 have been implicated in melanoma growth and angiogenesis ( R . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study , we examined the ontogeny of the type 1 receptor for basic fibroblast growth factor ( FGFR 1 ) in whole rat testis , its cellular localization , and its in vitro regulation in 20 day old rat Sertoli cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
There was significant correlation between the expression level of basic fibroblast growth factor ( basic FGF ) and that of FGFR 1 in tissues of human glioma ( Spearman ' s test , P < 0 . 05 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The findings that AIGF inhibited binding of [ 125I ] basic fibroblast growth factor ( bFGF ) and only FGF receptor ( FGFR ) 1 mRNA expressed in SC 3 cells suggested that AIGF acts through FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Using reverse transcription and the polymerase chain reaction , we have obtained novel evidence for the expression of three mRNAs encoding receptor tyrosine kinases in the adult rat testis : the platelet derived growth factor type A receptor ( PDGF RA ) , the basic fibroblast growth factor receptor ( flg ) , and fetal liver kinase 1 ( Flk 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The role of heparin or heparan sulfates in the interaction of basic fibroblast growth factor ( bFGF ) with its high affinity receptor were investigated using purified extracellular ligand binding region of FGF receptor 1 ( FGFR 1 ) and intact receptors expressed in a myeloid cell line ( 32D ) that does not express detectable levels of heparan sulfate proteoglycans or in Chinese hamster ovary ( CHO ) cell mutants defective in heparan sulfate synthesis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The aim of this study was to identify whether abnormalities in the synthesis of basic fibroblast growth factor ( bFGF ) or its receptor ( bFGF R ) were responsible for the photoreceptor dystrophy in Royal College of Surgeons ( RCS ) rats . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Using bovine retinal capillary endothelial cells ( BRCEC ) , we investigated the effects of short term hypoxia on DNA synthesis , phosphotyrosine induction , changes in the expression of basic fibroblast growth factor receptor ( bFGFR ) , protein kinase C ( PKC alpha ) , heat shock protein 70 ( HSP 70 ) , and SH 2 containing protein ( SHC ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
On six human hepatocellular carcinoma ( HCC ) cell lines ( KIM 1 , KYN 1 , KYN 2 , KYN 3 , HAK 1A , and HAK 1B ) , we examined expressions and functions of the proteins and messenger RNAs ( mRNAs ) of basic fibroblast growth factor ( bFGF ) and its receptor , i . e . , fibroblast growth factor receptor 1 ( FGFR 1 ) , as well as mRNA expressions of FGFR 2 approximately 4 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Angiogenic growth factors and their receptors , such as basic fibroblast growth factor ( bFGF ) and Flg , vascular endothelial growth factor ( VEGF ) and Flk 1 , platelet derived growth factor ( PDGF ) B chain and PDGF beta receptor , and five intracellular signal proteins ( phosphatidylinositol 3 kinase [ PI3K ] , phospholipase C gamma [ PLC gamma ] , C Src , SHC , and mitogen activated protein kinase [ MAPK ] ) were examined by Western blot analysis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
H 202 induced SMC proliferation was strongly and specifically inhibited by a neutralizing monoclonal antibody directed against basic fibroblast growth factor ( bFGF ) but was not due to increased expression of bFGF or the bFGF receptor 1 ( FGFR 1 ) by SMC . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of basic fibroblast growth factor ( bFGF ) and its receptor , the high affinity type 1 basic fibroblast growth factor receptor ( FGFR 1 ) : were immunohistologically studied in tissues specimens from 167 patients with a pulmonary adenocarcinoma . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In order to determine functional roles of basic fibroblast growth factor ( FGF 2 ) in the peripheral nervous system we have analysed the expression of FGF 2 and FGF receptor 1 ( FGFR 1 ) in spinal ganglia and the sciatic nerve under normal conditions and after nerve crush using RNAse protection assay and in situ hybridization . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Tumor specimens of non small cell lung carcinomas ( NSCLC ) from previously untreated patients ( n = 153 ) were analysed immunohistochemically for expression of vascular endothelial growth factor ( VEGF ) , VEGF receptor ( Flt 1 ) , basic fibroblast growth factor ( bFGF ) and FGF receptor ( FGFR 1 , ( Flg ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have studied the expression of basic fibroblast growth factor 2 ( FGF 2 ) and FGF receptor 1 ( FGFR 1 ) in the hypoglossal motor system during degeneration and regeneration by using an RNase protection assay , in situ hybridization , and Western blot analysis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Form deprivation did not affect the levels of basic fibroblast growth factor receptor 1 messenger RNA in retina / retinal pigment epithelium / choroid ( Northern blotting ) , but it abolished the decrease in amount of extractable basic fibroblast growth factor normally observed in the dark ( Western blotting ) . ^^^ The results are discussed with respect to previous findings on basic fibroblast growth factor 2 and basic fibroblast growth factor receptor 1 localization in the avian and other vertebrate eyes , and their relevance to form deprivation myopia . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have studied separated normal human breast epithelial and myoepithelial cells for the presence of basic fibroblast growth factor ( FGF 2 ) and its receptors , both low ( heparan sulfate proteoglycans ) and high affinity ( FGFR 1 ) , and for the effects of FGF 2 on the proliferation of both cell types . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Unlike normal melanocytes , primary and metastatic human melanomas express high levels of basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 ( FGFR 1 ) messenger RNA , and expression of these genes is essential in sustaining the proliferation of malignant melanomas in vitro . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We characterized the survival factor response of the normal mouse retina to mechanical injury by examining the expression of mRNAs for basic fibroblast growth factor ( bFGF ) , ciliary neurotrophic factor ( CNTF ) , and their receptors , FGF receptor 1 ( FGFR 1 ) and CNTF receptor alpha ( CNTFR alpha ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Prognostic value of basic fibroblast growth factor and its receptor ( FGFR 1 ) in patients with non small cell lung carcinomas . ^^^ Tumour specimens of 206 patients with untreated non small cell lung carcinomas ( NSCLC ) were analysed immunohistochemically for the expression of the basic fibroblast growth factor ( bFGF ) and for its receptor ( FGFR 1 , Flg ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Herein , we report the expression and localization of both basic fibroblast growth factor ( FGF 2 ) and its receptor ( FGF R 1 ; flg ) in human endometrium and demonstrate the markedly decreased FGF R 1 levels in menorrhagia . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To determine if bFGF and basic fibroblast growth factor receptor 1 ( bFGFR 1 ) and 2 ( bFGFR 2 ) are upregulated in head and neck squamous cell carcinoma ( HNSCC ) , we measured the distribution and levels of each in HNSCC specimens and control specimens . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The microvascular density ( MVD ) was determined by the factor 8 related antigen ( F8RA ) , and the basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 ( FGFR 1 ) protein expressions were immunologically studied with the MVD . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study , the RT PCR method was used to determine the gene expression of basic fibroblast growth factor ( bFGF ) , keratinocyte growth factor ( KGF ) , FGF receptor 1 ( FGFR 1 ) , FGF receptor 2 ( FGFR 2 ) , and KGF receptor ( KGFR ) in mouse embryos and in the stromal and epithelial cells of the uterine endometrium . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Phosphatidylcholine ( PC ) hydrolysis induced by basic fibroblast growth factor ( bFGF ) was studied in rat L 6 myoblasts expressing the wild type FGF receptor 1 ( FGFR 1 ) or a mutant ( Y766F ) that is incapable of activating phospholipase C gamma ( PLCgamma ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Interactions of putative heparin binding domains of basic fibroblast growth factor and its receptor , FGFR 1 , with heparin using synthetic peptides . ^^^ We have examined structure function relationships that have been proposed to account for the heparin binding properties of basic fibroblast growth factor and its receptor , FGFR 1 , using synthetic peptides , DNA synthesis assays and binding assays in a resonant mirror biosensor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
These mechanisms include cell density phenomena such as cell cell contact as well as signaling of basic fibroblast growth factor ( FGF 2 ) and its receptor ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
PD 166866 was a potent inhibitor of basic fibroblast growth factor ( bFGF ) mediated receptor autophosphorylation in NIH 3T3 cells expressing endogenous FGFR 1 and in L 6 cells overexpressing the human FGFR 1 tyrosine kinase , confirming a tyrosine kinase mediated mechanism . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
This study was undertaken to biochemically and immunohistochemically clarify the expression of basic fibroblast growth factor ( bFGF ) , insulin like growth factor 1 ( IGF 1 ) , fibroblast growth factor receptor ( bFGFR ) , insulin like growth factor 1 receptor ( IGF IR ) and vascular smooth muscle cell ( VSMC ) growth by using Streptozotocin ( STZ ) treated rat serum . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Archival specimens of 51 intestinal type and 38 diffuse type human gastric carcinomas were examined for tumor vessel counts , angiogenic factor expression , and the presence or absence of angiogenic factor receptors on tumor endothelium using antibodies against vascular endothelial growth factor ( VEGF ) and its receptors ( KDR and flt 1 ) , basic fibroblast growth factor ( bFGF ) and its receptors ( bek and flg ) , and factor 8 ( endothelial cells ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , we investigated the effect of chronic alcohol ingestion on the interplay between the receptor bound basic fibroblast growth factor ( bFGF R ) and the expression of cyclin dependent kinase ( Cdk 2 ) in buccal mucosa during ulcer healing . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
It is previously reported that basic fibroblast growth factor ( bFGF ) , major ligand to FGFR 1 , was overly expressed in human malignant astrocytomas . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , we analyzed the interplay between mucosal expression of the receptor bound basic fibroblast growth factor ( bFGF R ) and cyclin dependent kinase ( Cdk 2 ) , and the activity of apoptotic protease , caspase 3 , and constitutive nitric oxide synthase ( cNOS ) during chronic gastric ulcer healing . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Furthermore , AAV infection of permissive cells , known to express both FGFR 1 and the epidermal growth factor receptor , is abrogated by treatment of cells with basic fibroblast growth factor , but not with epidermal growth factor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Alterations in expression of basic fibroblast growth factor ( FGF ) 2 and its receptor FGFR 1 in human prostate cancer . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Expression of basic fibroblast growth factor and its receptors FGFR 1 and FGFR 2 in human benign prostatic hyperplasia treated with finasteride . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( bFGF ) , via activation of its receptor , FGFR 1 , has been postulated to be an important inducer of host stromal response and angiogenesis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Moreover , we have analyzed ES lines carrying targeted mutations for fibroblast growth factor receptor 1 ( fgfr 1 ) , a receptor for basic fibroblast growth factor ( bFGF ) , as well as scl , a transcription factor , for their potential to generate BL CFCs and Flk 1 ( + ) cells , to further define events leading to hemangioblast development . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The compounds were ATP competitive inhibitors of c Src kinase with IC ( 50 ) values < 10 nM and from 6 to > 100 fold selectivity for c Src tyrosine kinase relative to basic fibroblast growth factor receptor ( bFGFr ) tyrosine kinase , platelet derived growth factor receptor ( PDGFr ) tyrosine kinase , and epidermal growth factor receptor ( EGFr ) tyrosine kinase . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunolocalization of transforming growth factor alpha ( TGF Alpha ) , epidermal growth factor ( EGF ) , insulinlike growth factor ( IGF ) 1 , vascular endothelial growth factor ( VEGF ( 165 , 189 , 121 ) ) , basic fibroblast growth factor ( FGF ) 2 , EGF receptor ( R ) , IGF IRbeta , and FGFR 1 was studied in uterine leiomyomas and matched myometrial samples taken from seven women ( 42 47 years of age ) in the proliferative phase of the menstrual cycle . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
BACKGROUND : Two prominent biological features of the advanced stages of human melanoma are their high degree of vascularity and high level expression of basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study , we examined a potential role for PLC gamma 1 in the basic fibroblast growth factor ( bFGF ) induced PAK translocation using stable PC 12 cell lines that overexpress in a tetracycline inducible manner either the wild type FGFR 1 or the Y766F FGFR 1 mutant . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
BACKGROUND : To clarify the significance of basic fibroblast growth factor ( bFGF ) in angiogenesis or proliferative activity in cardiac myxoma , the expression of bFGF and its receptor ( FGFR 1 ) were immunohistochemically examined . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Granulosa cells and thecal tissues in small ( diameter , < 4 mm ) , medium ( diameter , 4 5 mm ) , or large ( diameter , > 5 mm ) individual follicles were collected for detection of mRNA expression of vascular endothelial growth factor ( VEGF ) 120 , VEGF 164 , basic fibroblast growth factor ( bFGF ) , and epidermal growth factor ( EGF ) in granulosa cells and fms like tyrosine kinase ( Flt 1 ) , fetal liver kinase ( Flk 1 ) or the murine homologue of kinase domain region ( KDR ) , bFGF receptor ( bFGF R ) , and EGF receptor ( EGF R ) in thecal tissue by semiquantitative reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The aim of this study was to assess the subcellular localization of basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 ( FGFR 1 ) in pituitary adenomas . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
These cells predominantly expressed fibroblast growth factor receptor type 1 ( FGFR 1 ) , and a minority exhibited basic fibroblast growth factor ( bFGF ) , whereas few expressed epidermal growth factor receptor ( EGFR ) or EGF . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , we demonstrate that SPARC does not bind to basic fibroblast growth factor ( bFGF / FGF 2 ) or interfere with complex formation between FGF 2 and its high affinity FGF receptor 1 ( FGFR 1 ) , yet both native SPARC and a peptide derived from the C terminal high affinity Ca ( 2+ ) binding region of protein significantly inhibit ligand induced autophosphorylation of FGFR 1 ( > 80 % ) , activation of mitogen activated protein kinases ( MAPKs ) ( > 75 % ) , and DNA synthesis in human microvascular endothelial cells ( HMVEC ) stimulated by FGF 2 ( > 80 % ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
However , DNA synthesis and phosphorylation of fibroblast growth factor receptor 1 ( FGFR 1 ) induced by thrombin or FXa are inhibited by antibodies neutralizing basic fibroblast growth factor ( bFGF ) or by heparin . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , expression of angiogenic factors , vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , VEGF receptors , kinase insert domain containing region ( KDR ) , and bFGF receptor Flg was characterized at the maternal embryonic boundary of the rhesus monkey on Day 17 , 19 , 28 and 34 of pregnancy . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( bFGF ) and its receptor expression ( bek and flg ) In bone marrow stroma of murine AIDS . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
OBJECTIVE : To study the expressions of basic fibroblast growth factor ( bFGF ) and its receptor ( bFGFR ) in bone marrow of mice with acute radiation injury , and to evaluate the effect of Ligustrazine ( Lt ) on them . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , the expression of vascular endothelial growth factor ( VEGF ) , basic fibroblast growth factor ( bFGF ) , and VEGF receptors , fms like tyrosine kinase ( Flt 1 ) and kinase insert domain containing region ( KDR ) , and bFGF receptor 1 ( Flg ) were examined in the endometrium of rhesus monkey on Day 5 , 10 , 16 , 20 , 25 of menstrual cycle and on Day 19 of early pregnancy . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The levels of basic fibroblast growth factor ( bFGF ) and its receptor Flg , vascular endothelial growth factor ( VEGF ) and VEGF / KDR complex ( 11B5 ) , and platelet derived growth factor ( PDGF A ) and its receptor PDGFR alpha , and alpha smooth muscle actin ( alpha SMA ) were determined in specimens with immuneohistochemical staining . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Because heparin inhibits PDGF mediated proliferation in human SMCs , we investigated whether the heparin binding growth factor basic fibroblast growth factor ( bFGF ) and one of its receptors , FGFR 1 , play a role in the response of human arterial SMCs to PDGF BB . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
T47D cells were inoculated into a 24 well plate , co transfected with luciferase vector OB HRE containing HIF 1 functional sequence ( HRE ) and pRL SV 40 ( as inner marker ) , pretreated with SU 5402 , inhibitor of FGFR 1 , SB 203580 , inhibitors of PI 3K , PD 98059 , inhibitors of MEK 1 , or LY 294002 , inhibitors of p 38 , treated with basic fibroblast growth factor ( bFGF ) , and then lysed . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( bFGF ) is an important mitogen and neurotrophic factor that binds and signals through the high affinity receptor , fibroblast growth factor receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have previously shown that human SMCs , when stimulated with thrombin , release basic fibroblast growth factor ( bFGF ) , causing phosphorylation of FGF receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Basic fibroblast growth factor ( bFGF ) and its major receptor FGF receptor 1 ( FGFR 1 ) play an important role in the development of the cortex . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The distribution and expression of ciliary neurotrophic factor ( CNTF ) and basic fibroblast growth factor ( FGF 2 ) and their receptor elements FGFR 1 and CNTFRalpha were studied with immunohistochemistry and Western blot analysis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 ( FGFR 1 ) in human meningiomas and the relationships between their expression and the tumors ' histological features and angiogenesis were investigated by means of immunohistochemical technique . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
HABP stimulated proliferation of lung fibroblasts MRC 5 was accompanied by a significant intracellular increase in basic fibroblast growth factor ( bFGF ) , the major ligand of FGFR 1 ; bFGF could however not be identified in the supernatant of HABP treated cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To examine the effects and potential implications for the expression of the two basic fibroblast growth factor ( bFGF ) receptors , FGFR 1 and FGFR 2 , in retinal pigment epithelial ( RPE ) cells , bFGF dependent changes in gene expression and RPE cell function were studied . bFGF increased L type Ca2+ channel activity of RPE cells , which in turn resulted in an increase of vascular endothelial growth factor A ( VEGF A ) secretion from RPE cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
OBJECTIVE : To investigate the immunocytochemical expression and presence of mRNA transcripts for basic fibroblast growth factor ( bFGF ) and its four receptors ( FGFR 1 , 2 , 3 , and 4 ) in ovaries from human adults and fetuses . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Although basic fibroblast growth factor ( bFGF ) is a known endothelial mitogen , the prognostic role of bFGF and its receptor FGFR 1 in prostate cancer has been controversial . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We analyzed the expression of basic fibroblast growth factor ( bFGF ) and nerve growth factor ( NGF ) and their receptor ( bFGFR and NGFR ) like immunoreactivity in chick basilar papillae , along with bFGF and NGF mRNA expression . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To increase transgene activity in in vitro glioma cells with low or deficient levels of CAR , the authors used basic fibroblast growth factor ( FGF 2 ) as a targeting ligand to redirect adenoviral infection through its cognate receptor , FGF receptor 1 ( FGFR 1 ) , which was expressed at high levels by all glioma cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
BACKGROUND : The aim of this study was to evaluate the relationship between the expression of basic fibroblast growth factor ( bFGF ) and fibroblast growth factor receptor 1 ( FGFR 1 ) in cancer cells and fibroblasts at the invasive front of oral squamous cell carcinoma ( OSCC ) , and the pathologic and clinical characteristics . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In addition , inhibition of FGFR 1 tyrosine kinase blocked basic fibroblast growth factor ( bFGF ) activation of ERK1 / 2 , but did not affect activation of ERK1 / 2 by clustered L 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
METHODS : There were 21 specimens of lumbar intervertebral discs from 15 patients with discogenic low back pain during posterior lumbar interbody fusion , 16 aging discs from patients without low back pain , and 10 normal discs as control collected for the study of their histopathologic features , as well as the expressions of basic fibroblast growth factor ( bFGF ) and its receptor ( Flg ) , transforming growth factor beta 1 ( TGF beta 1 ) and its receptor ( TGF betaRI ) by immunohistochemistry . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The second part of experiment was to observe the dynamic expression of early growth response protein ( Egr 1 ) , endogenous basic fibroblast growth factor ( endo bFGF ) and bFGF receptor ( bFGFR ) using immunohistochemistry after exo bFGF is administered to brain . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We studied the localization and transcriptional expression of bFGF and FGF receptor ( flg ) , heparin binding growth factor ( HBGF ) activity , localization of lysosomal enzymes and alpha 1 antitrypsin ( AAT ) , and electron microscopy of the normal mouse visceral yolk sac ( VYS ) and AVY . bFGF , which is normally present in the endoderm of the VYS of 8 day old embryos and in all components of the VYS by Day 11 of gestation , was reduced in the AVY . ^^^ The mRNA for bFGF was not detectable in either VYS or AVY , while flg mRNA was detected equally in both organs in Northern blotting . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
A candidate bFGF receptor , which is the product of the flg gene and which may recognize the int 2 protein , is expressed at high levels in HC 11 cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Expression of high affinity bFGF receptor ( flg ) mRNA was found to be temporally regulated . flg 4 . 3 kb mRNA expression was high in embryonic rat brain ( E 13 E19 ) . ^^^ There appears to be coordinate expression between the 1 . 8 kb bFGF mRNA and flg . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Direct binding experiments with radiolabeled acidic FGF ( aFGF ) or basic FGF ( bFGF ) , inhibition of binding with native growth factors , and Scatchard analysis of the binding data indicated that bek and flg bind either aFGF or bFGF with dissociation constants of ( 2 15 ) 10 10 ( 11 ) M . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FDC P 1 cells expressing FGFR 1 bound aFGF and bFGF with high affinity but only in the presence of heparin . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To identify injury responsive factors and cells , we examined the mRNAs for the following factors and some of their receptors : basic and acidic fibroblast growth factors ( bFGF , aFGF ) and FGF receptor 1 ( FGFR 1 ) ; ciliary neurotrophic factor ( CNTF ) and CNTF receptor alpha ( CNTFR alpha ) ; brain derived neurotrophic factor ( BDNF ) and its receptor trkB ; and insulin like growth factor 1 ( IGF 1 ) and IGFR 1 receptor ( IGF 1R ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF , bFGF and flg mRNAs show distinct patterns of induction in the hippocampus following kainate induced seizures . ^^^ We report that kainic acid induced seizures lead to marked increases in mRNAs encoding basic and acidic fibroblast growth factors ( bFGF and aFGF , respectively ) and flg , one of their receptors , in the rat hippocampus . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The binding interactions for the three primary reactants of the fibroblast growth factor ( FGF ) system , basic FGF ( bFGF ) , an FGF receptor , FGFR 1 , and the cofactor heparin / heparan sulfate ( HS ) , were explored by isothermal titrating calorimetry , ultracentrifugation , and molecular modeling . ^^^ The binding reactions were first dissected into three binary reactions : ( 1 ) FGFR 1 + bFGF < == > FGFR1 / bFGF , K 1 = 41 ( + / 12 ) nM ; ( 2 ) FGFR 1 + HS < == > FGFR1 / HS , K 2 = 104 ( + / 17 ) microM ; and ( 3 ) bFGF + HS < == > bFGF / HS , K 3 = 470 ( + / 20 ) nM , where HS = low MW heparin , approximately 3 kDa . ^^^ The first , binding of bFGF to FGFR 1 in the absence of HS , was found to be a simple binary binding reaction that is enthalpy dominated and characterized by a single equilibrium constant , K 1 . ^^^ The conditional reactions of bFGF and FGFR 1 in the presence of heparin were then examined under conditions that saturate only the bFGF heparin site ( 1 . 5 equiv of HS / bFGF ) or saturate the HS binding sites of both bFGF and FGFR 1 ( 1 . 0 mM HS ) . ^^^ Both 3 and 5 kDa low MW heparins increased the affinity for FGFR 1 binding to bFGF by approximately 10 fold ( Kd = 4 . 9 + / 2 . 0 nM ) , relative to the reaction with no HS . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Expression of the mouse derived cDNA encoding FGF receptor 1 ( FGFR 1 ) in MDCK cells resulted in the acquisition of a fibroblast like morphology when the transfected cells were cultured at low density in the presence of 0 . 6 % fetal calf serum and 20 ng / ml bFGF . ^^^ Coexpression of bFGF and FGFR 1 in the MDCK cells resulted in similar morphological effects that were not dependent upon exogenous bFGF . ^^^ Incubation of monolayers of FGFR 1 transfected cells with bFGF resulted in the loss of trans epithelial resistance . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We also examined the expression of mRNAs for aFGF , bFGF , KGF , FGF receptor 1 ( FGFR 1 ) and FGFR 2 in epithelial and stromal cells using Reverse Transcriptase Polymerase Chain Reaction ( RT PCR ) analysis . ^^^ Furthermore , RT PCR analysis demonstrated the expression of mRNAs for bFGF , KGF and FGFR 1 in stromal cells and that of FGFR 2 in epithelial cells . ^^^ These findings suggest that bFGF produced by stromal cells acts on stromal cells through FGFR 1 by an autocrine mechanism , KGF produced by stromal cells acts on epithelial cells through FGFR 2 by a paracrine manner in human BPH . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To begin to analyze putative relationships of growth factor responsiveness and growth factor / growth factor receptor expressions , IMR 32 , GIMEN , and LAN 1 cell lines were studied for the presence of bFGF , NGF , FGF receptors ( R ) 1 ( flg ) and FGFR 4 , trk , and low affinity NGF receptor ( p 75 ) mRNAs . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Northern blots showed that the pulp cells expressed flg ( FGF receptor type 1 ) transcripts throughout all culture stages , irrespective of the presence or absence of bFGF . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunohistochemical analyses using antibodies against VEGF , bFGF , their receptors ( KDR , flt 1 , bek , and flg ) , factor 8 , and proliferating cell nuclear antigen were carried out on archival specimens of 52 human colon carcinomas and 10 adenomas . ^^^ Expression of VEGF and KDR was higher in metastatic than in nonmetastatic neoplasms and directly correlated with the extent of neovascularization and the degree of proliferation , whereas expression of bFGF , flt 1 , bek , and flg did not differ among tumor types . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Inhibition of the growth of a human nasopharyngeal carcinoma cell line by bFGF is mediated via FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
High affinity ( Kd , approximately 0 . 2 nM ) and low capacity ( approximately 500 6000 sites / cell , depending on luteal ages ) [ 125I ] bFGF binding sites ( mol wt , 140 kilodaltons , determined by affinity labeling ) were found on luteal cells . [ 125I ] bFGF binding to luteal cells and corpus luteum membranes progressively decreased in binding capacity without affecting binding affinity as the age of corpus luteum advanced . bFGF receptor ( flg ) mRNA in luteinized ovaries decreased with the luteal age similar to the [ 125I ] bFGF binding . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Adult myocytes were found to express short and long variants of bFGF receptor 1 ( FGFR 1 , tyrosine kinase ) mRNA . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGFR 1 ( flg ) and FGFR 2 ( bek ) were expressed more by fibroblasts and bFGF was expressed more by corneal than limbal epithelial cells . ^^^ In addition to these potential paracrine actions , autocrine actions mediated by TGF alpha / EGFR , IL 1 beta / IL1 R , and bFGF / FGFR 1 were more expressed by corneal than limbal epithelial cells . ^^^ FGFR 1 ( flg ) and FGFR 2 ( bek ) were expressed more by fibroblasts and bFGF was expressed more by corneal than limbal epithelial cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The bFGF receptor 1 FGFR 1 ) exists in short and long isoforms due to alternate RNA splicing . ^^^ In the absence of any sequence for heart FGFR 1 and accumulating evidence for a role of bFGF in heart growth and differentiation , we cloned FGFR 1 from embryonic mouse hearts . ^^^ The majority of FGFR 1 transcripts in the adult as well as embryonic heart contained exon IIIc ( FGFR 1 IIIc ) which is associated with isoforms that display the highest affinity for bFGF . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Parallel changes were observed with bFGF receptor ( flg ) mRNA . ^^^ Nuclear runoff assays confirmed increased transcription of both bFGF and flg genes in response to 85 % O 2 , whereas increased translation at 6 days of exposure was confirmed by protein immunoanalysis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
This is consistent with the A 90 cell line having higher levels of endogenous bFGF and flg and therefore being more responsive to suramin inhibition than the A 121 cell line . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To elucidate the mechanism of this bFGF action , we examined changes in FGF receptor ( FGFR ) mRNA ( flg ) expression with in situ hybridization . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To study the roles of bFGF and its receptor in the process of neuronal cell death and the wound repair response , we induced 10 min of transient global cerebral ischemia in rats and measured changes in expression of both bFGF and the FGF receptor , flg . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The molecular bases of this interaction were investigated by evaluating the capacity of conventional and selectively desulfated heparins 1 ) to affect the binding of bFGF to FGFR and HSPGs of NIH 3T3 cells transfected with FGFR 1 / flg cDNA , 2 ) to facilitate the interaction of bFGF with a recombinant soluble form of the extracellular domain of FGFR 1 / flg ( xcFGFR 1 ) , and 3 ) to protect xcFGFR 1 from tryptic cleavage . 6 O desulfated ( 6 O DS ) heparin , but not 2 O desulfated ( 2 O DS ) and N desulfated / N acetylated ( N DS / N Ac ) heparins , retains the capacity to bind bFGF , as assessed by its ability to inhibit bFGF binding to cell associated FGFR 1 and HSPGs . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , in order to clarify the targets of such raised endogenous bFGF levels , the messenger RNA ( mRNA ) expression of its receptors ( flg and bek ) in the hippocampus following transient forebrain ischemia induced by four vessel occlusion for 20 min was investigated using an in situ hybridization technique . ^^^ The time course of the appearance and distribution pattern of flg mRNA positive cells in the CA 1 subfield were quite similar to those of bFGF mRNA positive cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We observed a marked inhibition of binding of bFGF to the receptor when the acidic domain was completely deleted , but mutants with two and four amino acids deleted ( flg delta A 2 and flg delta A 4 , respectively ) still bound the ligand . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
At least two families of receptors bind bFGF and could mediate its response : ( 1 ) tyrosine kinase containing FGF receptors , designated FGFR 1 to FGFR 4 , and ( 2 ) heparan sulfate proteoglycans that bind bFGF through their heparan sulfate chains . ^^^ Transfecting these cells with FGFR 1 results in bFGF responsive cells , which bind and internalize bFGF through FGFR 1 , and are killed . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have examined expression of bFGF and fibroblast growth factor receptor 1 ( FGFR 1 ) in luminal SMCs as well as endothelium at various times after injury , which allowed us to study both replicating as well as quiescent cells . ^^^ These data provide evidence that in injured arteries the ligand / receptor system of bFGF and FGFR 1 may be involved in the continued proliferative response of SMCs leading to neointima formation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Unlike the heparin from which it was derived , however , the hexasaccharide can not promote the binding of 125I bFGF to a recombinant high affinity bFGF receptor ( flg ) or restore the bFGF dependent proliferative response to ACE cells grown in the presence of 5 mM sodium chlorate . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Cells predominantly of the ' contractile ' phenotype expressed low numbers of high affinity EGF and bFGF receptors ( EGFr : 1 . 09 + / 0 . 18 fmol / 10 ( 6 ) cells ; bFGFr : 0 . 32 + / 0 . 07 fmol / 10 ( 6 ) cells ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The increase in mRNA levels was followed by an increased synthesis of FGFR 1 protein , which responded to basic FGF ( bFGF ) stimulation with induction of kinase activity and biological signaling . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Exogenous heparin enhanced the specific binding and affinity cross linking of 125I bFGF to FGFR 1 in receptor transfectants that were not cotransfected with proteoglycan , but had no effect on this binding and decreased the yield of bFGFR cross links in cells that were cotransfected with proteoglycan . ^^^ In previous experiments , cell surface heparan sulfate proteoglycans extracted from human lung fibroblasts could not be shown to promote high affinity binding of bFGF when added to heparan sulfate deficient cells that express FGF receptor 1 ( FGFR 1 ) ( Aviezer , D . , D . ^^^ Finally , complementary cell free binding assays indicated that the affinity of 125I bFGF for an immobilized FGFR 1 ectodomain was increased threefold when the syndecan 4 ectodomain was coimmobilized with receptor . ^^^ We conclude that , at least in K 562 cells , syndecans and glypican can support bFGF FGFR 1 interactions and signaling , and that cell surface association may augment their effectiveness . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Exposure of the cells to bFGF resulted not only in FGFR 1 tyrosine phosphorylation , but also in tyrosine phosphorylation of phospholipase C gamma , one of the initial enzymes in the intracellular signaling cascade initiated by FGFR activation . ^^^ Although no definitive evidence for the presence of FGFR 1 in normal LHRH neurons could be obtained by either double immunohistochemistry or double in situ hybridization procedures , fetal LHRH neurons in primary culture responded to bFGF with neurite outgrowth . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The aim of this study was to determine whether expression of bFGF and its receptors ( FGFR 1 and 2 ) is altered in portal hypertensive esophageal mucosa , especially in the muscularis mucosal layer , which constitutes a physical barrier to variceal rupture . ^^^ In 30 PHT rats and 30 sham operated controls 2 weeks after operation , specimens of lower esophagus were obtained for ( 1 ) quantitative histologic assessment including thickness of epithelium and muscularis mucosae ; ( 2 ) immunostaining with specific antibodies against bFGF and its receptors 1 and 2 ( intensity of bFGF , FGFR 1 and FGFR 2 immunostaining in esophageal structures was measured with a video image system ) ; and ( 3 ) expression of bFGF and FGFR 1 and 2 mRNAs was assessed with reverse transcription polymerase chain reaction . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To determine the expression of bFGF and FGF receptor 1 ( FGFR 1 ) mRNA in mouse embryos and uterine endometrial epithelial cells , a reverse transcription polymerase chain reaction ( RT PCR ) technique was used . ^^^ In the mouse embryos , bFGF mRNA was not detected but FGFR 1 mRNA was expressed in the blastocyst stage . ^^^ Both bFGF and two forms of FGFR 1 mRNA were detected in mouse endometrial epithelial cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have screened normal oral mucosa and oral squamous cell carcinoma ( SCC ) for expression of bFGF by immunohistology and northern analysis and used RT PCR to look for transcripts for KGF and the high affinity FGF receptors FGFR 1 and FGFR 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Northern analysis showed increased expression for both VEGF receptors ( flk 1 , flt 1 ) as well as the bFGF receptor 1 ( FGFR 1 ) in the collateral perfused region compared with that in the normally perfused region . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Both thrombin and bFGF induced the message for bFGF R 2 3 fold . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The presence of mRNA for aFGF , bFGF , and three isoforms of VEGF , as well as their receptors , FGFR 1 , FGFR 2 , Flt 1 , and KDR , in vessel outgrowths and the parent vessel , as identified by RT PCR , strongly implicated aFGF , bFGF , and VEGF as having an important role in this neovascularization response . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Moreover , the data indicate that bFGF control of stromal cell proliferation is modulated via a specific isoform of FGFR 1 containing the three loop immunoglobulin like domain . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
One adduct , 29 , reduced the number of FGFR 1 receptors in human aortic smooth cells for bFGF on the cell surface , which suggested down regulation of FGFR 1 gene expression . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
IGF 1 increases bFGF induced mitogenesis and upregulates FGFR 1 in rabbit vascular smooth muscle cells . ^^^ These results suggest that IGF 1 may be capable of increasing the responsiveness of VSMC to bFGF through modulation of FGFR 1 . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The FGFR 1 ( flg ) receptor , which is recognized by aFGF and bFGF , was studied by RT PCR . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Using in situ hybridization , we studied mRNA expressions of bFGF and one of its receptors , FGF receptor 1 ( FGFR 1 ) , during wound repair of the rat retina after laser photocoagulation . ^^^ Gene expressions of bFGF and FGFR 1 were detected in the ganglion cell and inner nuclear layers of the normal adult rat retina . ^^^ On day 3 following laser photocoagulation , proliferating retinal pigment epithelial ( RPE ) cells of the lesion showed intense gene expressions of bFGF and FGFR 1 . ^^^ The finding of elevated gene expressions of bFGF and FGFR 1 after laser photocoagulation suggests the bFGF may be a factor in retinal wound repair . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Since intrinsic bFGF , released from the damaged tissue , can influence the healing response through receptors upregulated by injury , it is reasonable that this pattern of bFGF mRNA expression parallels the bFGFR mRNA expression previously reported . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We examined 20 epiretinal membranes from patients with proliferative diabetic retinopathy ( PDR ) ( n = 12 ) and proliferative vitreoretinopathy ( PVR ) ( n = 8 ) for the presence of bFGF peptide , fibroblast growth factor receptor 1 ( FGFR 1 ) and bFGF messenger ribonucleic acid ( mRNA ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this study , we performed an immunohistochemical analysis of FGF receptors 1 , 2 and 3 ( FGFR 1 , FGFR 2 and FGFR 3 , respectively ) in NCN and MM and compared their expression and localization with those of bFGF . ^^^ None of the 10 NCN that showed strong , homogeneous staining for bFGF expressed FGFR 1 or FGFR 3 proteins ; six weakly expressed FGFR 2 protein . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
These findings support the role of the bFGF ligand receptor system in the pathogenesis of leiomyoma related bleeding and may have implications for fertility and contraception since the differential FGFR 1 expression occurs in the peri implantation period of the early luteal phase . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Pancreatic cancers overexpress basic FGF ( bFGF ) and the type 1 FGF receptor ( FGFR 1 ) , and overexpression of bFGF has been correlated with decreased patient survival . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The unique proliferative response to bFGF coincided with upregulation of its receptor , fibroblast growth factor receptor ( fgfr 1 ) , and downregulation of erythropoietin receptor ( EpoR ) gene expression . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We determined the expression and localization patterns of bFGF and one of its receptor ( FGFR 1 ) in normal renal as well as in renal cancers . ^^^ Using bFGF and FGFR 1 antibody , the repairing method of antigen with microwave oven heating and LSAB immunohistochemistry we used in 36 cases of paraffin embedded renal cell carcinoma ( RCC ) and their paired normal renal tissues . ^^^ The expression of bFGF and FGFR 1 was nearly consistent . ^^^ The bFGF and FGFR 1 can be consistently expressed in normal renal and renal cancer tissues , reflecting that the expression and function of these substances were closely associated . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The cultured human vascular SMCs and endothelial cells expressed both FGFR 1 and EGFR with predominant perinuclear localization . bFGF and HBEGF demonstrated equally potent mitogenic effects on SMC proliferation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
CONCLUSIONS : Continuous exposure to bright light upregulates bFGF , CNTF , FGFR 1 and GFAP expression in the rat retina . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
As a preliminary step to assessing the potential for using bFGF or its analogs as therapeutic agents , the expression of fibroblast growth factor receptor 1 ( FGFR 1 ) in the SN of normal and PD cases was investigated immunohistochemically . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Transcripts for ErbB 3 ( a member of the EGF R subfamily ) , and bFGF R were present in all stages of oocyte maturation , after fertilization up to the 2 cell stage , and the blastocyst stage . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Molecular modeling studies on binding of bFGF to heparin and its receptor FGFR 1 . ^^^ We have used molecular modeling method to study the modes of binding of heparin or heparan sulfate proteoglycans ( HSPGs ) to bFGF that leads to the dimerization of FGF receptor 1 ( FGFR 1 ) and activation of receptor tyrosine kinase . ^^^ Homology model of FGFR 1 Ig D ( 2 ) D ( 3 ) domains was built to investigate the interactions between heparin , bFGF and FGFR 1 . ^^^ The structural requirements to bridge the two monomeric bFGF molecules by heparin or HSPGs and to simulate the dimerization and activation of FGFR 1 have been examined . ^^^ A structural model of the biologically functional dimeric bFGF heparin complex is proposed based on : ( a ) the stability of dimeric complex , ( b ) the favorable binding energies between heparin and bFGF molecules , and ( c ) its accessibility to FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Exogenously added acidic FGF ( aFGF ) , which generated a rapid tyrosine phosphorylation of FGFR 1 and FGFR 2 on KS cells , reversed the inhibitory effects of anti bFGF Ab . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In MCF 7 / NCF ( FGF ) ( 18 , 22 , 24 ) cells , FGFR 1 and MAP kinase were not phosphorylated at baseline nor upon stimulation with recombinant bFGF , and exogenous bFGF only had a minimal effect on low steady state p21WAF1 / CIP1 levels . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
All cells expressed bFGF and high affinity receptors for FGFs ( FGFR 1 and FGFR 3 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In regions that have undergone necrosis , there was complete loss of aFGF , bFGF , and FGFR 1 immunostaining . ^^^ In contrast , in the regenerating areas , aFGF and bFGF were readily evident in exocrine type cells , in association with a marked increase in FGFR 1 immunoreactivity . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Consistent with its growth suppressive effect , FGFR 1 antisense oligonucleotides markedly reduced expression of both FGFR 1 mRNA and high affinity bFGF binding sites , whereas FGFR 1 reverse antisense control oligonucleotide had no effect . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
A striking increase in the bFGF immunoreactivity and a moderate increase in the fibroblast growth factor receptor 1 ( FGFR 1 ) immunoreactivity were also found in the astrocytes treated with serum or glucose deprivation or glutamate . ^^^ The increased bFGF immunoreactivity and FGFR 1 immunoreactivity were mainly accumulated in the nuclei of astrocytes . ^^^ The results suggest that the expression of bFGF and FGFR 1 in the astrocytes , especially in the nuclear interior , can be up regulated under ischemic like conditions and that the up regulation of bFGF and FGFR 1 may play an important role in the maintenance and repair of the central nervous system ( CNS ) after ischemia . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Furthermore , transcript levels of FGF receptor 1 ( FGFR 1 ) and 4 ( FGFR 4 ) isoforms , as well as of aFGF and bFGF were assessed by RT PCR . aFGF treated myotubes displayed increased expression of aFGF and bFGF , suggesting a paracrine effect of exogenous aFGF . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
It also prevented the increase in protein tyrosine phosphorylation induced by bFGF , including phosphorylation of FGFR 1 , FGFR 2 , phospholipase C gamma ( PLC gamma ) , and Shc as well as the recruitment of the Grb2 / Sos signaling complex . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
METHODS : Confocal immunofluorescence microscopy was used to identify sites of bFGF and FGF receptor 1 ( FGFR 1 ) expression after focal injury or experimental retinal detachment in adult rats . ^^^ Reverse transcription of total RNA derived from dissociated rat photoreceptor cells , followed by amplification of FGFR 1 cDNA using the PCR , verified the presence of FGFR 1 transcripts in normal rat photoreceptor cells ; in contrast , no evidence of bFGF transcription was detected . ^^^ Within hours after experimental retinal detachment or focal injury , there is a twofold increase in FGFR 1 immunoreactivity in the outer nuclear layer that persists for at least 7 days ; a similar increase in bFGF immunoreactivity in the interphotoreceptor matrix is also apparent . ^^^ CONCLUSIONS : This body of evidence strongly suggests that the photoreceptor rescue effect elicited by retinal injury as well as by injection of exogenous bFGF is mediated , at least in part , by upregulation of the FGFR 1 by the photoreceptor cells . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Correlation of bFGF , FGFR 1 and VEGF expression with vascularity and malignancy of human astrocytomas . ^^^ In contrast , the expression of FGFR 1 was related to neither bFGF nor FVIIIRAg and had no significant effect on tumor malignancy . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Indirect immunohistochemistry was performed using the Strept ABC method with four antibodies : bFGF , KGF , FGF receptor ( flg ) , and KGF receptor ( bek ) . ^^^ There was absent or weak bFGF , KGF , flg , and bek immunoreactivity in normal kidneys . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Vessels were demonstrated by immunocytochemical labelling for CD 34 and expressed as raw counts and volume weighted counts . bFGF protein and its receptors FGFR 1 ( flg ) and FGFR 2 ( bek ) , were demonstrated using immunocytochemical labelling . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We examined immunohistochemically the fracture repair process in rat tibial bone using antibodies to PCNA , BMP 2 , TGF beta 1 , 2 , 3 , TGF beta R 1 , R 2 , bFGF , bFGFR , PDGF , VEGF , and S 100 . ^^^ Hypertrophic chondrocytes were also positive for these growth factors but negative for bFGF and bFGFR . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study we show that established ONB tumors treated with bFGF upregulate the bFGF receptor ( FGFR 1 ) prior to differentiation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
METHODS : The expression of bFGF , fibroblast growth factor receptor 1 ( FGFR 1 ) and fibroblast growth factor receptor 2 ( FGFR 2 ) was analyzed by immunohistochemistry and Western blot . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this work , the bFGF receptors ( FGFR 1 , FGFR 2 IIIc and FGFR 3 ) genes expression was evaluated to study the correlation between the expression of bFGF receptors and induction of BPH . ^^^ CONCLUSIONS : bFGF receptors and particularly FGFR 1 seem to be involved in the induction and evolution of BPH and probably potentiate bFGF over expression effects in BPH . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of NGF ( Trk A ) and bFGF ( flg ) receptors paralleled the switch in trophic requirement . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The actions of bFGF are mediated by four different transmembrane receptors ( FGFR 1 4 ) . ^^^ Among them , FGFR 1 has a high affinity for bFGF , and gain of function mutations of the FGFR 1 gene cause craniosynostosis in humans . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The mechanism of bFGF induced cell death in tumours of the Ewing ' s sarcoma family ( ESFT ) has been investigated . bFGF induces phosphorylation of FGFr 1 and activation of Ras / ERK in ESFT cells that die when exposed to bFGF . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of bFGF and FGFR 1 was examined by Northern blot , in situ hybridization and immunohistochemistry . ( 3 ) H TdR incorporation was used to detect the role of bFGF in promoting the proliferation of renal tubular epithelial cells and renal fibroblasts . ^^^ RESULTS : The expression of bFGF mRNA and protein synthesis of bFGF and FGFR 1 increased during the process of injury and regeneration of tubular epithelial cells . ^^^ CONCLUSION : bFGF is secreted by tubular epithelial cells damaged by nephrotoxicity of gentamicin , and FGFR 1 expressed in damaged renal tubules and interstitial fibroblasts . bFGF not only can promote tubular epithelia to proliferate , but can also stimulate interstitial fibroblasts to proliferate . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Experiments with neutralizing Abs to bFGF and an FGFR 1 tyrosine kinase inhibitor showed that this growth factor was the major ERK activating factor released after injury . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We performed quantitative immunohistochemistry for bFGF , mast cell tryptase , smooth muscle actin for smooth muscle cell / myofibroblast like cells , and fibroblast growth factor receptors ( Flg , Bek ) and measured collagen and elastic fiber in lung sections from IPF ( n = 14 ) , LAM ( n = 9 ) , and control lung ( n = 10 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In contrast , bFGF activated isolated avian OC bone pit resorption via mechanisms inhibited by a selective cyclo oxygenase ( COX ) 2 prostaglandin inhibitor ( NS 398 ) or p42 / p44 MAPK activation inhibitor ( PD 98059 ) , consistent with a relatively high expression of FGFR 1 by differentiated avian OCs . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RT PCR analysis revealed that HSPG mRNA and FGFR 1 and FGFR 2 ( tyrosine kinase receptors for bFGF ) mRNA are expressed in TM BBB 4 cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The biological significance of VEGF , EGF , and bFGF is mediated by their receptors , which belong to the family of tyrosine kinase receptors : Flt 1 ( VEGFR 1 ) , KDR ( VEGFR 2 ) , EGFR , FGFR 1 , FGFR 2 , FGFR 3 , and FGFR 4 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The biological activity of bFGF is mediated through interaction with its high affinity receptor , fibroblast growth factor receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunohistochemical assays for bFGF , fibroblast growth factor receptor 1 ( FGFR 1 ) , and proliferating cell nuclear antigen were performed . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RT PCR products for bFGF , bFGFR , VEGF , PDGF AA , PDGF BB , TGF beta , and beta actin were analyzed to study the mRNA expressions . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RT PCR showed mRNA expression of bFGF receptor 2 ( FGFR 2 ) IIIb , which does not bind bFGF efficiently , by 4T1 cells and B 16 cells express FGFR 1 but not FGFR 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Starting 3 days after surgery , fibroblast growth factor receptor 1 ( FGFR 1 ) positive cells appeared in the granular and spinous cell layers of the reepithelializing mucosa in the bFGF treated group , whereas almost none was observed in the intact oral mucosa . ^^^ However , the number and intensity of FGFR 1 positive cells in the bFGF treated group were greater than in the control group . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The response of bFGF , FGFR 1 , Bcl 2 and caspase 3 to visible light in cultured human retinal pigment epithelium cells ] . ^^^ OBJECTIVE : To assess the expression of bFGF , FGFR 1 , Bcl 2 and caspase 3 in human RPE cells exposed to visible light in vitro . ^^^ Immunocytochemical staining , enzyme linked assays and RT PCR were used to determine the expression of bFGF and its receptor FGFR 1 , Bcl 2 as well as the activity of caspase 3 . ^^^ The changes in expression of Bcl 2 mRNA paralleled to the changes in its protein , except that differences could be detected earlier viz . 12 hours after exposure . ( 2 ) The upregulation of bFGF and FGFR 1 mRNA expression occurred with longer period of light exposure ( P < 0 . 05 ) . ( 3 ) The upregulation of caspase 3 activity was detected 6 hours after light exposure , and was more significant over 6 hours and 12 hours ( P < 0 . 05 ) . ^^^ CONCLUSIONS : Visible light exposure over threshold causes upregulation of endogenous bFGF and FGFR 1 , downregulation of Bcl 2 and activation of caspase 3 in human RPE cells . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The effects of Ligustrazine on the expression of bFGF and bFGFR in bone marrow in radiation injured mice . ^^^ Expressions of bFGF and bFGFR were much higher in Ligustrazine treated group than that in the control group ( P < 0 . 05 or P < 0 . 01 ) . ^^^ Ligustrazine potentiate the expression of bFGF and bFGFR in bone marrow MNC to recover the bone marrow hematopoiesis inductive microenvironment , which is one of the mechanisms by which Ligustrazine rebuild the bone marrow hematopoiesis after acute radioactive injury . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Expression of aFGF , bFGF , and FGFR 1 in ovarian epithelial neoplasm . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of bFGF and its receptor FGFR 1 in rat after fluid percussive brain injury ] . ^^^ OBJECTIVE : Study on the pattern of changes of bFGF and FGFR 1 immunoreactivity occurred in the experimental brain injury model for the purpose of providing the scientific basis for molecular pathological diagnosis , forensic identification , clinical treatment as well as further ascertaining the molecular mechanism of brain injury . ^^^ The SP immunohistochemistry method was used to examine the expression of both bFGF and FGFR 1 factors in rat brain . ^^^ RESULTS : In the brain of normal control and sham operation control groups , the low expression levels of bFGF and FGFR 1 were observed . ^^^ The increase of bFGF and FGFR 1 immunoreactivity could be observed 6 h after injury in cortex and brain stem , reached to the peak at 1 d and remained at the high level up to 3 d , then partly declined at 7 d . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
VEGF R 2 and alphavbeta 3 were observed to co associate after stimulation with either L1Ig6 or VEGF A 165 , whereas no co association with bFGF R was observed . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
MM 14 cells express FGFR 1 and are repressed from differentiation by FGF 1 , FGF 2 , and FGF 4 but not FGF 7 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of a M ( r ) 150 , 000 protein resulted when MDA MB 134 cells were treated with FGF 1 or FGF 2 , implying the presence of a functional FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RT PCR analyses revealed FGF 1 , FGF 2 , FGFR 1 ( flg ) , and TGF beta 1 mRNA in all samples without evidence of modulation by FGF 1 affixation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Testosterone and FGF 2 increased the expression of FGFR 1 messenger RNA ( mRNA ) and , to a lesser extent , FGFR 3 mRNA , but down regulated FGFR 2 mRNA in S 115 cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The activated fibroblast growth factor receptor 1 ( FGFR 1 ) expressed in L 6 myoblasts did not bind Grb 2 directly , but indirectly , through the small adaptor protein Shc , which was tyrosine phosphorylated in response to fibroblast growth factor 2 ( FGF 2 ) stimulation . ^^^ FGF 2 stimulation of mutant FGFR 1 expressing cells still allowed formation of complexes containing Shc , Grb 2 , and the nucleotide exchange factor Sos and mediation of a mitogenic signal . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Epileptiform activity was induced by kainic acid injection , and in situ hybridization was used to assess the progress of changes in distribution and intensities of FGF receptor 1 ( FGFR 1 ) , FGF 5 , and FGF 2 mRNAs . ^^^ Immunohistochemistry showed an induction of FGFR 1 and FGF 2 , 6 h postseizure induction which remained elevated up to 24 h later . ^^^ The distinctive pattern of the FGFR 1 mRNA response appears to indicate that FGFR 1 is a factor in the modulation of the cellular response for FGF 5 and FGF 2 . ^^^ These results demonstrate that brain activity exerts influences at the gene expression levels of FGFR 1 and its ligands FGF 5 and FGF 2 . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In addition , expression of functional FGF high affinity receptors ( FGFR 1 ) did not enhance u PA in L 6 myoblasts upon FGF 2 stimulation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Thus , FGF 2LA bound , with the same affinity as FGF 1 and FGF 2 , to FGFR 1 and FGFR 2 , whereas only FGF 1 and the FGF 1 loop containing mutant , FGF 2LA , bound to the keratinocyte growth factor receptor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The same group of rats exhibited an increase in the density of cells displaying FGF 2 immunoreactivity and cells displaying FGFR 1 immunoreactivity and cells displaying FGFR 1 immunoreactivity , and also an induction of FGF 2 mRNA in the tissue surrounding the lesion . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In addition , the recombinant extracellular domain of FGFR 1 continues to bind to corneal endothelial cell matrix after endogenous FGF 2 has been removed with 2 M NaCl . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Unlike FGF 2 , the distribution of FGFR 1 mRNA correlates well with the presence of the immunoreactive receptor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
All ECL 2 neurons expressed FGFR 1 ; therefore , we tested the role of FGF 2 in the survival of glutamatergic ECL 2 neurons . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The time course of expression for FGF receptor 1 ( FGFR 1 ) was similar to FGF 2 with an increase in proliferating SMC . ^^^ Our data suggest that the FGF 2 / FGFR 1 system may play a role in the continued proliferation of intimal SMCs , while PDGF B may be promoting intimal lesion formation by stimulating SMC migration via its chemotactic effect . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
These results indicate that the transfected FGF 2 isoforms up regulated FGFR 1 mRNA and protein . ^^^ The 22 . 5 kDa form acted by increasing FGFR 1 mRNA stability enhancing cell responses to exogenous FGF 2 . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In order to clarify the physiological function of fibroblast growth factor ( FGF 2 ) in the adrenal medulla the regulation of FGF 2 and FGF receptor 1 ( FGFR 1 ) was studied in vitro and in vivo in response to glucocorticoids . ^^^ In contrast to the FGF 2 mRNA level FGFR 1 was not affected by treatment with glucocorticoids . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of FGF 1 , FGF 2 , and their receptor , FGF receptor 1 ( FGFR 1 ) , was examined in malignant salivary gland tumours and normal salivary glands , using immunohistochemical methods . ^^^ In seven cases of adenoid cystic carcinoma ( ACC ) , both duct like cells and modified myoepithelial cells were apparently immunopositive for FGF 1 , FGF 2 , and FGFR 1 . ^^^ In five cases of mucoepidermoid carcinoma ( MC ) , all three types of tumour cells including epidermoid cells , and intermediate cells expressed immunoreactive FGF 1 , FGF 2 , and FGFR 1 . ^^^ In these malignant salivary gland tumours , increased expression of FGFR 1 correlated with the intensity of both FGF 1 and FGF 2 immunoreactivity . ^^^ In contrast to malignant salivary gland tumours , eight cases of normal salivary gland showed negative immunostaining for FGF 1 , FGF 2 , and FGFR 1 while four cases were weakly immunoreactive for FGF and its receptor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To determine whether FGF receptors are also present within nuclei and if this localization is regulated by FGFs , nuclei were prepared from quiescent and FGF 2 treated Swiss 3T3 fibroblasts and examined for the presence of FGF receptors by immunoblotting with an antibody produced against the extracellular domain of FGF receptor 1 ( FGFR 1 ) . ^^^ When cells are treated with FGF 2 , however , there is a time and dose dependent increase in the association of FGFR 1 immunoreactivity with the nucleus . ^^^ When cell surface proteins are labeled with biotin , a biotinylated FGFR 1 is detected in the nuclear fraction prepared from FGF 2 treated , but not untreated , cells indicating that the nuclear associated FGFR 1 immunoreactivity derives from the cell surface . ^^^ The presence of FGFR 1 in the nuclei of FGF 2 treated cells was confirmed by immunostaining with a panel of different FGFR 1 antibodies , including one directed against the COOH terminal domain of the protein . ^^^ Fractionation of nuclei from FGF 2 treated cells indicates that nuclear FGFR 1 is localized to the nuclear matrix , suggesting that the receptor may play a role in regulating gene activity . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 expression is restricted to the epidermal placodes , whereas FGFR 1 expression is limited to the dermal condensations . ^^^ Treatment of sc / sc skins with FGF 2 results in the formation of feathers at the site of application of the growth factor and the induced feathers express FGFR 1 in their dermal condensations . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the blastocyst , FGFR 1 as well as binding of 125I FGF 2 showed a dramatic increase from day 6 . 0 to day 6 . 5 , coinciding with the onset of gastrulation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Western blot analysis demonstrated the presence of 103 , 118 , and 145 kDa forms of FGFR 1 in nuclei isolated from BAMC . 125I FGF 2 cross linking to nuclear extracts followed by FGFR 1 immunoprecipitation showed that FGFR 1 can account for the nuclear FGF 2 binding sites . ^^^ Agents that induce translocation of endogenous FGF 2 to the nucleus ( forskolin , carbachol , or angiotensin 2 ) increased the intranuclear accumulation of FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The results show that FGF 2 antagonists suramin , protamine , heparin , the synthetic peptide FGF 2 ( 112 155 ) , and a soluble form of FGFR 1 do not inhibit FGF 2 mediated uPA up regulation at concentrations that affect growth factor binding to cell surface receptors and mitogenic activity . ^^^ In contrast , tyrosine phosphorylation inhibitors and overexpression of a dominant negative TK mutant of FGFR 1 abolish the uPA inducing activity of FGF 2 , indicating that FGFR and its TK activity are essential in mediating uPA induction . ^^^ Accordingly , FGF 2 induces uPA up regulation in Chinese hamster ovary cells transfected with wild type FGFR 1 , 2 , 3 , or 4 but not with TK FGFR 1 mutant . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Ligand blot analysis using 125I labeled FGF 2 showed that a FGFBP of 46 48 kDa ( represented by a double band ) was present on the nuclear membrane at mid to late G 1 , and Western blot showed this to be immunologically related to a part of the extracellular domain of the high affinity FGF receptor 1 ( FGFR 1 ) . ^^^ The truncated FGFR 1 may mediate the nuclear translocation of FGF 2 during late G1 . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The distinct expression pattern of FGFR 1 in migrating and differentiating muscle cells indicates that in addition to the stimulation of proliferation of myoblasts , FGF 2 exerts other ( nonmitogenic ) effects on postmitotic myocytes . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
A comprehensive analysis of the distribution of FGF 2 and FGFR 1 in the rat brain . ^^^ We have examined the cellular distribution of both FGF 2 and FGFR 1 immunoreactivity and their mRNAs throughout the normal adult rat brain in order to reconcile numerous disparate findings in the published literature . ^^^ The results confirm a widespread distribution of FGF 2 and FGFR 1 in the rat brain , and different regions express distinct patterns of FGF 2 and FGFR 1 mRNA and protein : neuronal and non neuronal cells show different subcellular distributions that vary according to the area where they are located . ^^^ Astrocytes contain the highest levels of FGF 2 and FGFR 1 mRNAs , and characteristically , possess high levels of immunoreactive FGF 2 within the nucleus . ^^^ Amongst non neuronal cells , oligodendrocytes do not synthesize or contain significant levels of FGF 2 immunoreactivity however , they do express FGFR 1 mRNA . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We studied the anatomical distribution of messenger RNA encoding FGF 2 and one of its high affinity receptors , FGFR 1 , using in situ hybridization in a variety of human fetal tissues in early second trimester . ^^^ Both FGF 2 and FGFR 1 mRNA and proteins were found to be present in every organ and tissue examined , but with defined cellular localizations . ^^^ In skeletal muscle , both FGF 2 and FGFR 1 mRNA and peptides were present in differentiated fibers , and both co localized to proliferating chondrocytes of the epiphyseal growth plate . ^^^ FGF 2 and FGFR 1 mRNA and peptides were also present within cardiac or gastrointestinal smooth muscle . ^^^ Within the gastrointestinal tract FGF 2 mRNA and peptide were located in the submucosal tissue , whereas FGFR 1 was expressed within the overlying mucosa . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In this report , the levels of FGF 1 , FGF 2 , FGF 7 mRNA and their receptors FGFR 1 and FGFR 2 , were investigated during epidermal carcinogenesis in transgenic mice expressing the early region of the ' high risk ' papillomavirus type 16 ( HPV 16 ) under control of the human keratin 14 enhancer / promoter ( K 14 HPV16 transgenic mice ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 1 transcripts were more abundant in newborn SMCs whereas FGF 2 and FGFR 1 mRNA expression was higher in adult SMCs . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Prostatic stromal cells express primarily the FGFR 3 IIIc isoform and smaller amounts of FGFR 1 IIIc and FGFR 2 IIIc isoforms , which bind both FGF 1 and FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression pattern of the FGFR 1 transcript matches that of the expression of FGF 2 and of the mitotic activity in the developing and adult cortex . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The first series of experiments was designed to clarify the expression relationship between FGF 2 , FGF , receptor 1 ( FGFR 1 ) and alpha smooth muscle actin ( alpha SMA ) . ^^^ The first series involving in situ hybridization with FGF 2 and FGFR 1 cRNA probes , showed that these mRNAs were expressed in the mesangial areas during the proliferative phase ( days 4 7 ) . ^^^ These findings suggest that FGF 2 and FGFR 1 showed significant correlation with the phenotypic changes of MC . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunohistochemical localization of fibroblast growth factor 1 ( FGF 1 ) , FGF 2 and fibroblast growth factor receptor 1 ( FGFR 1 ) in pleomorphic adenoma of the salivary glands . ^^^ In this study , fibroblast growth factor 1 ( FGF 1 ) , FGF 2 , and fibroblast growth factor receptor 1 ( FGFR 1 ) were immunohistochemically analyzed in 10 patients with pleomorphic adenoma of the salivary gland by using specific monoclonal antibodies . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Fibroblast growth factor 2 ( FGF 2 ) and FGF receptor ( FGFR 1 ) immunoreactivity in embryonic spinal autonomic neurons . ^^^ Immunoreactivity to FGF 2 , probably the best characterized member of the FGF family ( FGF 1 to FGF 10 ) and of one of its high affinity receptors , FGFR 1 , was found in autonomic neurons at embryonic day E 14 , the peak day of generation and proliferation in the common ventral motoneuron pool . ^^^ Immunogold electron microscopy revealed the cytoplasmic localization of FGF 2 and FGFR 1 in intermediolateral neurons , the major group of sympathetic preganglionic neurons in the spinal cord . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In addition , the FGF 2 containing medium , which can not stimulate NBT 2 cells due to absence of appropriate receptors , is able to induce scattering of NBT 2 cells expressing the FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In contrast to levels of the FGFR 1 transcript , which is not altered , the level of FGF 2 mRNA is dramatically up regulated in spinal ganglia after injury . ^^^ The differential response of FGF 2 mRNA and protein and of FGFR 1 mRNA in spinal ganglia and sciatic nerve after lesion is suggestive of different physiological functions : a local reaction at the lesion site where axonal regrowth occurs and a trophic reaction for the degenerating / regenerating sensory neurons . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Moreover , analysis of FGF receptors evidenced a dramatic increase in FGFR 1 mRNA and protein level , supporting the hypothesis that this receptor mediates the transforming effect of FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We show here FGF 2 and FGF receptor type 1 ( FGFR 1 ) protein and mRNA expression in preganglionic intermediolateral neurons of the rat thoracic spinal cord . ^^^ While immunoreactivity of both FGF 2 and FGFR 1 co localize to intermediolateral neurons , mRNA transcripts of FGFR 1 , but not of FGF 2 , are detectable in intermediolateral preparations by RNase protection analysis , suggesting protein translocation in vivo . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Furthermore , immunocytochemical localization of receptors for FGF 2 ( i . e . , FGFR 1 ) on projecting basal forebrain cholinergic neurons suggests that FGF 2 acts directly on these neurons to induce the lesion induced sprouting response . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RG 13577 inhibited FGF 2 binding to soluble and cell surface FGF receptor 1 ( FGFR 1 ) . ^^^ Moreover , it abrogated heparin mediated dimerization of FGF 2 and FGFR 1 , as well as FGF 2 mitogenic activity in HS deficient F 32 lymphoid cells . ^^^ The antiproliferative effect of compound RG 13577 was associated with abrogation of FGF 2 induced tyrosine phosphorylation of FGFR 1 and of cytoplasmic proteins involved in FGF 2 signal transduction , such as p 90 and mitogen activated protein kinase . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunoprecipitation of total cell extracts with FGF receptor ( FGFR ) 1 4 antibodies showed that U251MG glioma cells express only FGFR 1 . [ 125I ] FGF 2 cross linking to nuclear extracts followed by FGFR 1 immunoprecipitation showed that FGFR 1 may account for the nuclear FGF 2 binding sites . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The signal transduction pathway leading to activation of p 70 ( s6k ) by fibroblast growth factor receptor 1 ( FGFR 1 ) was examined in FGF 2 treated rat L 6 myoblasts . p 70 ( s6k ) was activated in a biphasic and rapamycin sensitive manner . ^^^ Our results indicate that FGFR 1 mediates p 70 ( s6k ) activation by a phosphatidylinositol 3 ' kinase independent mechanism that does not require PKC activation and , furthermore , proliferation , but not differentiation of endothelial cells in response to FGF 2 , is associated with p 70 ( s6k ) activation . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We analysed the expression of FGF 2 and type 1 fibroblast growth factor receptor ( FGFR 1 ) in 20 selected cases of human pancreatic carcinoma ( PC ) in connection with proliferation of tumour cells and intratumour endothelial cells ( ECs ) , using immunohistochemistry and in situ hybridization ( ISH ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In contrast to the FGFR 2 kinase fused to the ectodomain of FGFR 1 , the FGFR 1 kinase failed initially to support a mitogenic response to FGF 2 in type 1 tumor cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 exerts its effects by binding to cell surface receptors ( FGFR 1 ) of the tyrosine kinase family . ^^^ In contrast to non transfected H9c2 cells , treatment of H9c2 cells stably expressing long FGFR 1 with FGF 2 for 24 h resulted in a slight increase ( 1 . 3 fold , p < 0 . 02 ) in cell number . ^^^ However , a greater response ( 1 . 5 fold , p < 0 . 0005 ) was observed with H9c2 cells stably expressing short FGFR 1 after treatment with FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Using L6E9 skeletal myoblasts , devoid of endogenous FGF receptors , which have been engineered to express either FGF receptor 1 ( FGFR 1 ) or FGF receptor 4 ( FGFR 4 ) , we have demonstrated that both receptors , known to be expressed in skeletal muscle cell precursors , were able to mediate uPA induction by FGF 2 , whereas serum stimulation was FGF receptor independent . ^^^ The induction of uPA by FGF 2 and serum in FGFR 1 and in FGFR 4 expressing myoblasts required the mitogen activated protein kinase pathway , since treatment of cells with a specific inhibitor of the mitogen activated protein kinase / extracellular signal regulated kinase 2 kinase , PD 98059 , blocked uPA promoter induction . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In parallel with FGF 2 , the nuclei of astrocytes contain the high affinity FGF 2 receptor , FGFR 1 . ^^^ Transfection of either FGF 2 or FGFR 1 into cells that do not normally express these proteins results in their nuclear accumulation and concomitant increases in cell proliferation . ^^^ A similar regulation of nuclear FGF 2 and FGFR 1 is observed in neural crestderived adrenal medullary cells and of FGF 2 in the nuclei of cerebellar neurons . ^^^ Thus , the regulation of the nuclear content of FGF 2 and FGFR 1 could serve as a novel mechanism controlling growth and proliferation of glial and neuronal cells . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Because the effects of FGF 1 and FGF 2 are predominantly mediated through the FGF receptor 1 ( FGFR 1 ) , its expression has also been examined . ^^^ Cytoplasmic staining for FGF 1 , FGF 2 , and FGFR 1 was scored on a scale of 0 ( no staining ) to 3 ( heavy staining ) and expressed as a percentage of total cells stained . ^^^ Confocal microscopy of immunofluorescent staining for FGF 1 , FGF 2 , and FGFR 1 in sections of multinodular goiter ( n = 3 ) and normal thyroid ( n = 3 ) provided quantitation of immunostaining . ^^^ These data show that enhanced expression of FGF 1 , FGF 2 , and FGFR 1 accompany thyroid hyperplasia and are not exclusively associated with the neoplastic state . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The purpose of this study was to quantify and localize the sites of expression of FGF 2 and its high affinity receptor , FGFR 1 , within placentae from normal term human pregnancies ( n=8 , 39 42 weeks ) , and pregnancies complicated by pregestational , type 1 diabetes ( n=8 , 36 40 weeks ) . ^^^ Tissues were collected immediately following delivery and were either snap frozen for RNA isolation , or fixed for either in situ hybridization using a 35S labelled cRNAs encoding human FGF 2 or FGFR 1 , or immunocytochemistry using antibodies against human FGF 2 or FGFR 1 . ^^^ Northern blot hybridization showed a significantly increased abundance of mRNAs for both FGF 2 and FGFR 1 in placentae from diabetic women compared to those from normal women . ^^^ Messenger RNA for FGFR 1 was similarly distributed to that encoding FGF 2 . ^^^ Immunocytochemistry revealed abundant FGF 2 and FGFR 1 peptides in villous vascular endothelial cells , and associated with the cell membranes of stromal tissues in placentae from control pregnancies . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To gain insight into the mechanism of inhibition , we have analyzed the interaction of PF 4 with FGF 2 / FGFR by using mutant heparan sulfate deficient Chinese hamster ovary ( CHO ) cells transfected with the FGFR 1 cDNA ( CHOm FGFR 1 ) and by examining the direct interaction with FGF 2 . ^^^ In the absence of heparin , PF 4 inhibited binding of 125I FGF 2 to CHOm FGFR 1 cells in a concentration dependent manner , although not completely . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of fibroblast growth factor type 2 ( FGF 2 ) , epidermal growth factor ( EGF ) , and their respective receptors FGFR 1 and EGFR , as well as the proliferation marker Ki 67 , was studied in both constituents of adamantinoma in serial sections of 25 cases by immunohistochemistry . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We studied the expression of both fibroblast growth factor 2 ( FGF 2 ) and FGF receptor 1 ( FGFR 1 ) in various histological types of human thyroid neoplastic and hyperplastic tissues to clarify the biological behavior of FGF 2 . ^^^ With immunohistochemical staining , both FGF 2 and FGFR 1 were frequently detected in human thyroid carcinoma ( 79 . 2 to 100 % and 80 to 100 % , respectively ) . ^^^ The difference between FGF 2 and FGFR 1 expression in adenomatous goiters was statistically significant ( P < 0 . 05 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Differential regulation of FGF 2 and FGFR 1 in rat cortical astrocytes by dexamethasone and isoproterenol . ^^^ We have used rat cortical astrocytes in culture to investigate the signaling pathways involved in the regulation of fibroblast growth factor 2 ( FGF 2 ) and one of its high affinity receptor FGF receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Responsiveness of developing dental tissues to fibroblast growth factors : expression of splicing alternatives of FGFR 1 , 2 , 3 , and of FGFR 4 ; and stimulation of cell proliferation by FGF 2 , 4 , 8 , and 9 . ^^^ To elucidate the roles of fibroblast growth factors ( FGF ) in tooth development , we have analyzed the expression patterns of fibroblast growth factor receptors ( FGFR ) in mouse teeth by in situ hybridization and studied the effects of FGF 2 , 4 , 8 , and 9 on cell proliferation in vitro by local application with beads on isolated dental mesenchymes . mRNAs of FGFR 1 , 2 , and 3 were localized by probes specific for the alternative splice variants IIIb and IIIc . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Localization of fibroblast growth factor 2 ( FGF 2 ) protein and the receptors FGFR 1 4 in normal human seminiferous epithelium . ^^^ This study demonstrates for the first time the distribution pattern of FGF 2 and the receptors FGFR 1 4 in the normal seminiferous epithelium of adult men . ^^^ This distribution pattern suggests that FGF 2 in spermatogonia is involved in the autocrine and paracrine regulation of the proliferation and differentiation of spermatogonia and spermatocytes via the receptors FGFR 1 , FGFR 3 and FGFR 4 . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
This paper focuses on the role of proteoglycans synthesized by embryonic cells , embryoglycans , in FGF 2 signaling via FGF receptor 1 ( FGFR 1 ) . ^^^ These findings demonstrate that embryoglycan ectodomains may act as negative regulators of FGF 2 induced ES cell proliferation , most likely through the FGFR 1 independent signaling pathway . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The localization of FGFR 1 is consistent with previous reports on the involvement of FGF 1 and FGF 2 in AD . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 and FGFR 1 mRNA levels greatly increased in light induced retinal degeneration . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 induces tyrosine phosphorylation of FGFR 1 and FGFR 2 and many cellular substrates including MAP kinases and stimulates proliferation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We assessed the time course of expression of PTN , MK and FGF 2 during EAE and determined the cellular origin of FGF 2 and FGFR 1 in normal spinal cord and during inflammatory demyelination . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Specificity of the targeting for high affinity FGF receptors was demonstrated by competing the targeted phage with FGF 2 , by the failure of FGF 2 targeted bacteriophage to transduce high affinity FGF receptor negative cells , and by their ability to transduce these same cells when stably transfected with FGFR 1 , a high affinity FGF receptor . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Thus , the localization of FGFR 1 in carcinoma cells supports the hypothesis that an important part of FGF 2 binding reflects binding to FGFR 1 . bFGFR concentrations were positively correlated to estrogen receptor and progesterone receptor levels . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Analysis of the high affinity receptors of FGF 2 ( FGFR 1 and FGFR 3 ) and the mitogen activated protein kinase did not show any increase in either FGFR tyrosine phosphorylation or in mitogen activated protein kinase activation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Here we report that in the absence of flanking sequences , isolated Ig module 2 of FGFR 1 supports the binding of FGF 1 , FGF 2 , and FGF 7 in respective order of affinity . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Accordingly , GT1b , GD1b , GM 1 , and GM 2 , but not GM 3 and asialo GM 1 , prevent the binding of 125I FGF 2 to a soluble , recombinant form of extracellular FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Here , we investigated the axotomy induced regulation of FGF 2 and FGF receptor 1 ( FGFR 1 ) expression in the autonomic nervous system using the sympathetic superior cervical ganglion of the adult rat as a model . ^^^ In contrast to FGF 2 , expression of FGFR 1 was unchanged in ganglia after axotomy . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
PTH also increased FGF 2 mRNA , and FGFR 1 and FGFR 2 mRNA levels within 30 minutes in neonatal mouse calvarial organ cultures . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGFR type 4 ( FGFR 4 ) in liver parenchymal cells binds only FGF 1 , whereas FGFR 1 binds FGF 1 and FGF 2 equally . ^^^ However , in contrast to FGFR 1 , when recombinant FGFR 4 was expressed back in epithelial cells by transfection , it failed to bind FGF 2 unless heparan sulfate was depressed by chlorate or heparinase treatment . ^^^ Isolated heparan sulfate proteoglycan ( HSPG ) from liver cells in cell free complexes with FGFR 4 restored the specificity for FGF 1 and supported the binding of both FGF 1 and FGF 2 when complexed with FGFR 1 . ^^^ In contrast , FGF 2 bound equally well to complexes of both FGFR 1 and FGFR 4 formed with endothelial cell derived HSPG , but the endothelial HSPG was deficient for the binding of FGF 1 to both FGFR complexes . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
They also abrogated the formation of the HSPG / FGF 2 / FGFR ternary complex , as evidenced by their capacity to prevent FGF 2 mediated cell cell attachment of FGFR 1 overexpressing , HSPG deficient Chinese hamster ovary cells to wild type HSPG bearing cells . ^^^ The antiproliferative activity of these compounds was associated with the abrogation of FGF 2 induced tyrosine phosphorylation of FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
This is based on the following observations : PF 4 peptide 47 70 inhibited FGF 2 or VEGF binding to endothelial cells ; it inhibited FGF 2 or VEGF binding to FGFRs or VEGFRs in heparan sulfate deficient CHO cells transfected with FGFR 1 ( CHOFGFR 1 ) or VEGFR 2 ( CHOmVEGFR 2 ) cDNA ; it blocked proliferation or tube formation in three dimensional angiogenesis assays ; and , finally , it competed with the direct association of ( 125 ) 1 PF 4 with FGF 2 or VEGF , respectively , and inhibited heparin induced FGF 2 dimerization . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
BACKGROUND : The expression pattern of fibroblast growth factor 2 ( FGF 2 ; basic FGF ) , a pleiotrophic growth factor , as well as one of its receptors ( FGFR 1 ) , in the kidney is highly controversial . ^^^ METHODS : Using an approach that combines multiple antibodies for immunohistochemistry and correlative in situ hybridization , we assessed the intrarenal expression of both FGF 2 and FGFR 1 in 13 specimens of adult kidney removed during tumor nephrectomy . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The crystal structure of FGF 2 bound to a naturally occurring variant of FGF receptor 1 ( FGFR 1 ) consisting of immunoglobulin like domains 2 ( D 2 ) and 3 ( D 3 ) has been determined at 2 . 8 A resolution . ^^^ Two FGF 2 : FGFR 1 complexes form a 2 fold symmetric dimer . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We investigated the expression of FGF 2 and FGF receptors ( FGFR 1 to 4 ) in NSCLC cell lines ( N = 16 ) , NSCLC surgical specimens ( N = 21 ) and 2 control cell lines . ^^^ Expression levels of FGFR 1 and 2 in NSCLC cell lines correlated with FGF 2 production . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Overexpression of long or short FGFR 1 results in FGF 2 mediated proliferation in neonatal cardiac myocyte cultures . ^^^ OBJECTIVE : The type 1 fibroblast growth factor receptor ( FGFR 1 ) is the only high affinity receptor for fibroblast growth factor 2 ( FGF 2 ) in the rat myocardium , and is essential for normal growth and development of the heart . ^^^ To determine whether this response was FGF 2 specific , the level of FGF 2 was assessed in the culture medium of cardiac myocytes overexpressing FGFR 1 isoforms . ^^^ CONCLUSION : Overexpression of either FGFR 1 ( L ) or ( S ) resulted in an increase in FGF 2 mediated proliferation of postnatal rat cardiac myocytes . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Subcutaneous insertion of FGF 2 soaked beads onto the coronal suture on E 15 resulted in up regulation of osteopontin and Fgfr 1 in the sutural mesenchyme , down regulation of Fgfr 2 , and inhibition of cell proliferation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGFR 1 bound well to FGF 2 but poorly to FGF 8 and FGF 9 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Although no significant differences in the expression of adhesion molecules ( NCAM ) , selected morphogens ( TGFbeta 2 , HGF / SF , FGF 2 , KGF ) , or their receptors ( TGFbetaR 2 , m met , FGFR 1 ) were seen between DP of p75NTR knockout and wild type mice , p75NTR mutants showed a prominent upregulation of FGFR 2 , a high affinity receptor for KGF , in both follicular DP and epithelium . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We demonstrated the involvement of an autocrine loop involving endogenous FGF 1 in the mechanisms that govern FGF 2 induced resistance to apoptosis by showing : ( 1 ) higher levels of apoptosis in cells treated with antisense FGF 1 oligonucleotide or after neutralization of excreted FGF 1 ; ( 2 ) the long term activation of FGFR 1 and of ERK 2 , ( 3 ) the inhibition of FGFR 1 and ERK 2 activation and an increase in apoptosis if excreted FGF 1 was neutralized . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The comparison between 5 d stimulated hypothyroid TA muscle and 5 d stimulated TA and SOL derived satellite cell cultures revealed differences in the expression of FGF 1 and FGF 2 , but similar expression levels of FGFR 1 and FGFR 4 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The fibroblast growth factor ( FGF ) family , with its prototype members acidic FGF ( FGF 1 ) and basic FGF ( FGF 2 ) , binds to four related receptor tyrosine kinases , termed FGFR 1 , R 2 , R 3 , and R 4 , expressed on most types of cells in tissue culture . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The purified 33 kDa FGFR 1 IIIa fragment fusion protein could bind [ 125I ] labelled FGF 2 in Western ligand blot analysis . ^^^ Exposure to FGF 2 caused an increase in [ 3H ] thymidine incorporation into DNA of HL 60 cells and increased cell proliferation , but the addition of FGFR 1 IIIa fragment fusion protein inhibited FGF 2 stimulated DNA synthesis and caused a dose dependent inhibition of FGF 2 stimulated cell proliferation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Thus , the chemotactic activities of FGF 2 can be mediated by the low affinity FGFR alone or by a combination of low and high affinity FGFRs ( FGFR 1 , FGFR 3 , or both ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Co immunoprecipitation and in vitro binding assays demonstrated that binding of Grb 14 to FGFR 1 in mammalian cells was dependent on receptor activation by fibroblast growth factor 2 ( FGF 2 ) . ^^^ Expression of exogenous Grb 14 inhibited FGF 2 induced cell proliferation , whereas a point mutated form of Grb 14 incapable of binding to FGFR 1 enhanced FGF 2 induced mitogenesis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Furthermore , we show these isoforms are preferentially modified with heparan rather than chondroitin sulfate , bind the macrophage derived growth factors FGF 2 , vascular endothelial growth factor , and heparin binding epidermal growth factor with varying affinities ( K ( d ) 25 330 nM ) and in the case of FGF 2 , can stimulate productive binding to the high affinity tyrosine kinase FGF receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Transfected L 6 clones expressed respective proteins and bound ( 125 ) 1 labeled FGF 2 with K ( d ) values of 99 pm ( FGFR 1 ) and 26 pm ( FGFR 1L ) . ^^^ FGF 1 and FGF 2 competed efficiently with ( 125 ) 1 FGF 2 for binding to FGFR 1 and FGFR 1L , whereas FGF 4 was less efficient . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Seven primary cortical fibroblast lines were established , and expression of FGF 2 and FGF receptor 1 ( FGFR 1 ) were examined . ^^^ Primary cortical fibroblasts express FGF 2 and FGFR 1 in vitro . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the presence of FGF 2 , FGFR 1 kinase and subsequent mitogen activated protein kinase Erk 2 activities were augmented in a dose dependent manner , whereas high concentrations of heparin resulted in decreased activity . ^^^ The N and 2 O sulfate groups of heparin were essential for binding to FGF 2 , whereas stimulation of FGFR 1 and Erk 2 kinases by FGF 2 also required the presence of 6 O sulfate groups . ^^^ Thus , formation of a ternary complex of FGF 2 , heparin , and FGFR 1 appears critical for the activation of FGFR 1 kinase and downstream signal transduction . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To elucidate the structural determinants governing specificity in fibroblast growth factor ( FGF ) signaling , we have determined the crystal structures of FGF 1 and FGF 2 complexed with the ligand binding domains ( immunoglobulin like domains 2 [ D 2 ] and 3 [ D 3 ] ) of FGF receptor 1 ( FGFR 1 ) and FGFR 2 , respectively . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
TMPP demonstrated potent inhibition of binding of soluble FGF receptor 1 ( FGFR 1 ) to FGF 2 immobilized on heparin at submicromolar concentrations . ^^^ TMPP inhibits binding of radiolabeled FGF 2 to FGFR in a cell free system as well as to cells genetically engineered to express FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Cross linking demonstrated that FGF 2 / HSGAGB primarily activated FGFR 1 , which in turn up regulated the activity of mitogen activated protein kinase ; in contrast , FGF 1 / HSGAGA led to the phosphorylation of equal proportions of both FGFR 1 and FGFR 2 , which in turn led to the up regulation of Src and p 125 ( FAK ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We show here that Fgf 2 is expressed in the pseudostratified ventricular epithelium ( PVE ) in a dorsoventral gradient and that Fgf 2 and its receptor , Fgfr 1 , are downregulated by mid to late stages of neurogenesis . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 ( > / =10 ( 12 ) m ) up regulated the phosphorylation of cellular proteins , including p42 / p44 mitogen activated protein ( MAP ) kinase , and increased the kinase activity of immunoprecipitated FGFR 1 in mouse osteoclasts . ^^^ These results strongly suggest that FGF 2 acts directly on mature osteoclasts through activation of FGFR 1 and p42 / p44 MAP kinase , causing the stimulation of bone resorption at physiological or pathological concentrations . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The effects of FGF 9 and FGF 2 on FGFR and myelin protein levels were comparable ; both up regulated FGFR 1 , and down regulated FGFR 2 , CNP , PLP and MBP . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The FGFR 1 inhibitor PD 173074 selectively and potently antagonizes FGF 2 neurotrophic and neurotropic effects . ^^^ In the present study , we show that the FGFR 1 inhibitor PD 173074 potently and selectively antagonized the neurotrophic and neurotropic actions of FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunohistochemistry of FGF 1 , FGF 2 , FGFR 1 and p 53 used avidin biotin detection of the primary antibody . ^^^ These tumors expressed FGF 1 , FGF 2 , and FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To study possible interactions of both factors we have analyzed mutual effects of exogenous IL 6 and FGF 2 on the expression of each other and the corresponding receptor ( R ) molecules IL 6R and FGFR 1 after peripheral nerve lesion in vivo and in vitro using cultured Schwann cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Vascular endothelial growth factor ( VEGF ) and its receptors VEGFR 1 and VEGFR 2 , fibroblast growth factor 2 ( FGF 2 ) and its receptors FGFR 1 and FGFR 2 , as well as epidermal growth factor ( EGF ) and its receptor EGFR are believed to be important in the control of angiogenesis in the human endometrium . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of PTHrP , PTHrP receptor , Bcl 2 , FGF 2 , FGFR 1 , FGFR 3 , and p 21 is investigated by immunohistochemistry in osteochondromas ( n = 24 ) and peripheral ( n = 29 ) and central ( n = 20 ) chondrosarcomas . ^^^ In chondrosarcomas , re expression of FGF 2 , FGFR 1 , PTHrP , Bcl 2 , and p 21 is found . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The mitogen activated protein kinase ( MAP kinase ) signalling cascade activated by fibroblast growth factors ( FGF 1 and FGF 2 ) was analysed in a model system , Xenopus oocytes , expressing fibroblast growth factor receptors ( FGFR 1 and FGFR 4 ) . ^^^ Stimulation of FGFR 1 by FGF 1 or FGF 2 and FGFR 4 by FGF 1 induced a sustained phosphorylation of extracellular signal regulated protein kinase 2 ( ERK 2 ) and meiosis reinitiation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The following antibodies were used as the first antibody ; FGF 2 and FGF 7 for FGFs , FGFR 1 and FGFR 2 for FGFRs , NCAM , L1CAM , and N cadherin for CAMs . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Heparin as low as 0 . 01 mg / mL significantly downregulated expression of TGF beta ( 1 ) and FGF 1 stimulated FGF 2 and FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Here , we evaluated the effects of different heparan sulfate proteoglycan ( HSPG ) expressing cell lines and HSPGs derived from these cells on FGF 2 induced FGFR 1 phosphorylation in heparan sulfate negative BaF 3 cells . ^^^ HSPGs supplied in membrane associated form , by presenting cells , were all effective promotors of FGF 2 initiated FGFR 1 phosphorylation , independently of their nature ( syndecan / glypican ) or cellular origin ( human lung fibroblasts , transfected Namalwa cells , or transfected K 562 cells ) . ^^^ In comparison , equivalent amounts of soluble HSPGs , obtained by trypsinization of these cells or by immunopurification from cell extracts , did not promote FGF 2 induced FGFR 1 phosphorylation , yet removal of the less anionic species or a further treatment with heparitinase converted these soluble fractions into potent activators of FGF2 / FGFR1 signaling . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 induces long term activation of FGFR 1 and ERK1 / 2 , and production of the anti apoptotic protein BcL 10 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We demonstrate that the molecule , 5 , 10 , 15 , 20 tetrakis ( methyl 4 pyridyl ) 21H , 23H porphine tetra p tosylate salt ( TMPP ) , inhibits binding of radiolabeled FGF 2 to SMCs and to soluble FGF receptor 1 ( FGFR 1 ) in a manner that interferes with both ligand and receptor interactions with heparin , thereby blocking growth factor mediated SMC proliferation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
For this study , we examined the role of HSPGs as modulators of FGF 2 binding to FGFR 1 in situ and in vitro . ^^^ During stepwise reconstitution of the FGF 2 / HSPG / FGFR 1 complex in situ , we identified an elevated ability of breast carcinoma cell HSPGs to promote receptor complex formation compared to normal breast epithelium . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
However , targeted inhibition of extracellular FGF 2 by overexpression of a secreted receptor mutant FGFR 1 ( TM ) lacking the transmembrane domain failed to affect BMP 7 induced dendritic growth , as did treatment with the extracellular FGFR antagonist inositol hexakisphosphate . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
An FGF 2 mutant with diminished affinity for the tyrosine kinase FGF 2 receptor 1 ( FGFR 1 ) was not cardioprotective . ^^^ We conclude that intramyocardial FGF 2 administration shortly after the onset of ischemia confers protection from acute and chronic cardiac dysfunction and damage ; FGF 2 delivered during reperfusion protects from ischemia reperfusion injury ; and protection by FGF 2 requires intact binding to FGFR 1 and is likely mediated by PKC . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In parental L 6 cells that express exceedingly low levels of high affinity FGFRs , transduction with AdLuc was enhanced 7 to 10 fold with the FGF 2 Fab ' conjugate , whereas in L 6 cells transfected to express FGFR 1 , it was enhanced 39 to 52 fold . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Finally , FGFR 1 , but not TK ( ) DeltaFGFR 1 , localizes in cell adhesion contacts on immobilized FGF 2 . ^^^ In conclusion , substrate bound FGF 2 induces endothelial cell proliferation , motility , and the recruitment of FGFR 1 in cell substratum contacts . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Here we show that stimulation of angiotensin 2 ( AII ) receptors , depolarization , or activation protein kinase C ( PKC ) or adenylate cyclase all lead to nuclear accumulation of fibroblast growth factor 2 ( FGF 2 ) and FGFR 1 , association of FGFR 1 with splicing factor rich domains , and activation of the tyrosine hydroxylase ( TH ) gene promoter in bovine adrenal medullary cells ( BAMC ) . ^^^ Mechanism of TH gene activation by FGF 2 and FGFR 1 was further investigated in BAMC and human TE 671 cultures . ^^^ Activation of the TH promoter by nuclear FGFR 1 and FGF 2 was mediated through the cAMP responsive element ( CRE ) and was associated with induction of CREB and CBP / P 300 containing CRE complexes . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
METHODS : To quantify the expression of fibroblast growth factor receptor isoforms FGFR 2 ( IIIb ) , FGFR 2 ( IIIc ) , FGFR 1 ( IIIc ) , and fibroblast growth factors FGF 2 and FGF 7 in normal and tumoral human prostate tissues , and human prostatic epithelial cell lines , we used quantitative real time polymerase chain reaction . ^^^ On the other hand , neither FGFR 1 ( IIIc ) nor FGF 2 and FGF 7 have shown any significant variation in expression between normal and cancerous specimens . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We found that levels of mRNA for the FGFR 1 were attenuated in the mutant mice by the age of 6 months , whereas the mRNAs for FGF 1 and FGF 2 were reduced or unchanged in the brain regions tested . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Chondrogenesis was consistently induced when mutant FGFR 1 K656E or FGFR 2 C278F were electroporated in ovo into stage 8 quail premigratory neural crest , followed by in vitro culture without FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
However , the reversible changes found in FGFR 1 expression in the sclera of myopic eyes mean that an indirect role for FGF 2 in the control of scleral remodelling is implicated . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Monoclonal antibody ( MAb ) VBS 1 , produced against native fibroblast growth factor receptor 1 ( FGFR 1 ) , inhibited the binding of fibroblast growth factor 2 ( FGF 2 ) to its receptor on coronary venular endothelial cells ( CVECs ) as determined by 125I FGF 2 Scatchard analysis and [ 3H ] thymidine uptake assays ( ED 50 = 80 ng / mL ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
However , when compared to heparin it showed a reduced capacity to induce FGF 2 dimerization and to favor the interaction of [ 125I ] FGF 2 with FGFR 1 in HSPG deficient , FGFR 1 transfected CHO cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunocytochemical studies demonstrated that in vivo hair cells express FGF 2 and the receptors FGFR 1 and FGFR 2 while supporting cells do not express either molecule . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunoreactivity for receptors ( R ) for IGF 1 and FGF 2 ( IGF 1R , FGFR 1 , and FGFR 3 ) was most pronounced in chondroblasts and hypertrophic chondrocytes , while FGFR 2 immunoreactivity was strongest in the articular and prechondroblastic zones . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 potently upregulated uPA production in murine brain capillary endothelial cells ( IBE cells ) , as well as porcine aortic endothelial ( PAE ) cells and L 6 myoblasts ectopically expressing FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In order to investigate which receptor ( s ) may mediate FGF 2 signaling and their role in regeneration , we have studied expression of the four fibroblast growth factor receptors , FGFR 1 , FGFR 2 , FGFR 3 and FGFR 4 , and of the spliced variants , sFGFR and KGFR , in the regenerating spinal cord of the adult urodele , Pleurodeles waltl , following tail amputation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study , we have characterized the ability of FGFR 1 deficient mouse embryonic stem ( ES ) cells to bind FGF 2 and to proliferate in the absence or presence of exogenous FGF 2 . ^^^ We show that the targeted disruption of FGFR 1 leads to a reduced binding of FGF 2 which has no significant effect on the proliferation of undifferentiated ES cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To detect more dynamic , functional components of the epithelial mesenchymal trophic unit , we evaluated the distribution of perlecan , fibroblast growth factor 2 ( FGF 2 ) , and fibroblast growth factor receptor 1 ( FGFR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We assessed the role of fibroblast growth factors ( FGF ) in pulmonary vascular remodeling by examining the expression pattern of FGF 1 , FGF 2 , and the FGF receptor ( FGFR 1 ) in peripheral area of lung tissues from patients with COPD ( FEV ( 1 ) < or = 75 % ; n = 15 ) and without COPD ( FEV ( 1 ) > or = 85 % ; n = 13 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The expression of fibroblast growth factor 2 ( FGF 2 ) and fibroblast growth factor receptor 1 ( FGFR 1 ) in two cases of SC of the temporomandibular joint ( TMJ ) were immunohistochemically studied . ^^^ The possible roles of FGF 2 and FGFR 1 in SC of the TMJ are discussed . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Mono and disaccharide containing glycopolymers were synthesized by two different free radical processes , and their ability to act as heparan sulfate glycomimetics in promoting the binding of Fibroblast Growth Factor 2 ( FGF 2 ) to its receptor ( FGFR 1 ) was evaluated using an in vitro cell based assay . ^^^ In vitro assays demonstrate that specific glycopolymers can potentiate FGF 2 / FGFR 1 binding interactions . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Increased amounts of HSPG were present in the dermal nodules of scleromyxedema compared to adjacent normal dermis and these bound FGF 2 , immobilized the soluble receptor protein FGFR 1 and , therefore , formed a ternary complex composed of HSPG , FGF 2 and FGFR 1 in vitro . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The biological effects of FGF 2 in the myocardium are mediated by the high affinity tyrosine kinase receptor FGFR 1 , the major FGF receptor in the heart . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 stimulation induced translocation of the FGF type 1 receptor ( FGFR 1 ) into nuclei , suggesting that the growth stimulating effect of FGF 2 was mediated through FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RESULTS : RT PCR revealed that GE cells express FGFR 1 , FGFR 2 , FGFR 3 , and FGFR 4 mRNA ; however , not that of FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Using semiquantitative reverse transcription polymerase chain reaction , we examined the expression of insulin like growth factor 1 ( Igf 1 ) , fibroblast growth factor 2 ( Fgf 2 ) , and their receptors ( Igf 1r , Fgfr 1 , Fgfr 2 , and Fgfr 3 ) in the MCC of 28 day old rats at 3 , 7 , and 14 days after placing intraoral appliances designed to produce a lateral functional shift of the mandible . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Thus , FGF 2 , 4 , and 8 , found in RTF , may act upon FGFR 1 IIIc in the principal cells of the initial segment to regulate GGT mRNA 4 expression . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Our studies have shown that full length FGF receptor 1 ( FGFR 1 ) accumulates within the nuclear interior in parallel with FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The objective of this study was to elucidate the correlation between the expression of FGF 2 , VEGF , and their receptors FGF receptor 1 ( FGFR 1 ) and VEGF receptor 1 ( Flt 1 ) with microvessel density in synovial tissues of the TMJ . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
However , Namalwa cells , immobilizing FGF 2 via their cell surface CD44vRA , bound substantially more soluble FGF receptor 1 ( FGFR 1 ) than did Namalwa cells immobilizing the same amount of FGF 2 via their cell surface CD44v3 v 10 . ^^^ Finally , isolated primary synovial fluid cells from RA patients expressing CD44vRA bound more soluble FGFR 1 to their cell surface associated FGF 2 than did corresponding synovial cells expressing CD44v3 v 10 or synovial cells from osteoarthritis patients . ^^^ Hence , FGF 2 attached to the heparan sulfate moiety expressed by the novel CD 44 variant of RA synovium cells exhibits an augmented ability to stimulate FGFR 1 mediated activities . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Thyroidal levels of fibroblast growth factor 2 ( FGF 2 ) and fibroblast growth factor receptor 1 ( FGFR 1 ) are elevated in human thyroid hyperplasia . ^^^ To understand the significance of this , effects of FGFR 1 activation on normal human thyrocyte growth and function in vitro and the regulation of FGF 2 and FGFR 1 expression have been examined . ^^^ Sensitivity to FGF 2 disappeared after 7 days , although FGFR 1 expression was maintained . ^^^ Exogenous FGF 2 decreased FGFR 1 protein . ^^^ We conclude that TSH stimulates FGFR 1 but not FGF 2 expression . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We have studied the mechanism whereby heparin directs FGF 2 induced FGF receptor 1 ( FGFR 1 ) signal transduction . ^^^ FGF 2 alone stimulated maximal phosphorylation of Src homology domain 2 tyrosine phosphatase ( SHP 2 ) and the adaptor molecule Crk , in heparan sulfate deficient Chinese hamster ovary ( CHO ) 677 cells expressing FGFR 1 . ^^^ Analysis of tryptic phosphopeptide maps of FGFR 1 from cells stimulated with FGF 2 alone and FGF 2 together with heparin showed that FGF 2 alone stimulated a several fold increase in tyrosine 463 in the juxtamembrane domain . ^^^ Our data imply that tyrosine 463 is phosphorylated and able to transduce signals in response to FGF 2 treatment alone ; furthermore , we suggest that FGFR 1 dimerization / kinase activation is stabilized by heparin . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Similarly , T 3 enhanced FGF 2 activated autophosphorylation of FGFR 1 , but did not modify FGF 2 induced phosphorylation of the docking protein FRS 2 . ^^^ In situ hybridization analyses of TRalpha knockout mice , which have impaired ossification and skeletal mineralization , revealed reduced FGFR 1 mRNA expression in osteoblasts and osteocytes , whereas T 3 failed to stimulate FGFR 1 mRNA or enhance FGF 2 activated MAPK signaling in TRalpha null osteoblasts . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We also measured components of the FGF 2 ternary signaling complex [ fibroblast growth factor receptor 1 ( FGFR 1 ) and syndecan 4 ] . ^^^ These changes are associated with specific alterations in the regulation of FGF 2 , FGFR 1 , and syndecan 4 in the airway epithelial mesenchymal trophic unit , which may be associated with the developmental problems of lungs associated with exposure to ozone . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Levels of fibroblast growth factor 2 ( FGF 2 ) and its receptor , FGFR 1 , are elevated in goiter , but whether this is a direct effect of TSH is unknown . ^^^ We have determined the regulation of FGF 2 and FGFR 1 synthesis by TSH in a rat thyroid cell line ( FRTL 5 ) and have used a replication defective adenovirus ( RAd ) expressing dominant negative FGFR 1 ( RAdDN FGFR 1 ) to examine the role of FGFR signaling in vitro and in goiter induced in mice . ^^^ TSH induced FGF 2 and increased the expression of FGFR 1 in FRTL 5 cells . ^^^ Infection of TSH stimulated FRTL 5 cells with RAdDN FGFR 1 inhibited growth and prevented FGF 2 mediated inhibition of ( 125 ) 1 uptake . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To address how FGF 2 and fibroblast growth factor receptor 1 ( FGFR 1 ) gene expression influences skeletal muscle development and growth , pectoralis major muscle was isolated at embryonic days ( ED ) 14 , 16 , 18 , 20 , 22 , and 24 , and at 1 , 8 , 12 , and 16 week posthatch from a turkey line ( F ) selected only for increased 16 week body weight and its genetic control line ( RBC 2 ) . ^^^ The mRNA levels of FGF 2 and FGFR 1 were measured by semi quantitative reverse transcription polymerase chain reaction . ^^^ Compared to the RBC 2 line males , the F line males had higher FGF 2 mRNA levels at ED 14 and 16 , and higher FGFR 1 mRNA levels at ED 18 , but down regulated FGF 2 and FGFR 1 gene expression at ED 22 . ^^^ Although no FGF 2 mRNA was detected in posthatch muscle tissue , the F line turkeys had more FGFR 1 gene expression at 8 , 12 , and 16 week posthatch than the RBC 2 line turkeys . ^^^ During myogenic satellite cell proliferation , the F line cells had higher FGF 2 and FGFR 1 mRNA levels than the RBC 2 line cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
There was no evidence from immunohistochemistry for up regulation of receptors flg and bek for FGF 2 . ^^^ The presence of FGF 2 , flg and bek in arterioles may indicate a possible role for FGF 2 in the regulation of blood flow since we have previously shown it to be a dilator of small arterioles . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
On the surface of smooth muscle cells there are two types of receptors for the mitogenic and angiogenic growth factor fibroblast growth factor 2 ( FGF 2 ) ; a high affinity tyrosine kinase FGF receptor ( FGFR 1 ) and low affinity heparin . / heparan like glycosaminoglycan ( HLGAG ) component of surface expressed proteoglycans . ^^^ It is believed that all three components ; FGFR 1 , FGF 2 , and the HLGAG chains , must form a ternary complex for maximal cellular stimulation . ^^^ We report that heparinase treatment of bovine smooth muscle cells inhibits the binding of ( 125 ) 1 FGF 2 to FGFR 1 , but does not inhibit FGF 2 induced cellular proliferation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Epicardium is required for the full rate of myocyte proliferation and levels of expression of myocyte mitogenic factors FGF 2 and its receptor , FGFR 1 , but not for transmural myocardial patterning in the embryonic chick heart . ^^^ Consistent with this , FGFR 1 and FGF 2 are more highly expressed in myocytes of the compact zone . ^^^ Consistent with this , the expression patterns of FGF 1 , FGF 2 , and FGFR 1 across the myocardium persist in the absence of the epicardium . ^^^ However , FGF 2 and FGFR 1 mRNA levels are reduced in proportion to the depletion of epicardium . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
OBJECTIVE : To study the modulating effect of sensory neuropeptide substance P ( SP ) on gene expression of epidermal growth factor ( EGF ) , fibroblast growth factor 2 ( FGF 2 ) and EGF receptor ( EGFR ) , FGF receptor 1 ( FGFR 1 ) in granulation fibroblast . ^^^ SP content and EGF , EGFR , FGF 2 , FGFR 1 gene expression in the granulation tissue were observed using immunohistochemistry and in situ hybridization combined with image analysis on 3rd , 6th , 9th 12th after skin injury , respectively . ^^^ SP concentration was from 10 ( 9 ) mol / L to 10 ( 5 ) mol / L for upregulation of FGF 2 mRNA expression in cultured fibroblasts and from 10 ( 6 ) mol / L to 10 ( 5 ) mol / L for upregulation of FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Upon cell stimulation , fibroblast growth factor receptor 1 ( FGFR 1 ) , a typically plasma membrane associated protein , is released from ER membranes into the cytosol and translocates to the cell nucleus by an importin beta mediated transport pathway along with its ligand , FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Multiple studies have shown that migration , proliferation , and differentiation of oligodendrocyte ( OL ) lineage cells are influenced by fibroblast growth factor 2 ( FGF 2 ) signaling through its receptors ( FGFR ) FGFR 1 , FGFR 2 , and FGFR 3 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RESULTS : A construct encoding the FGFR 1 ectodomain produced secreted sFGFR 1 protein , which bound [ ( 125 ) 1 ] FGF 2 with high affinity and specificity , and inhibited FGF 2 stimulated 3T3 fibroblast proliferation in vitro . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The response to FGF 2 upregulation was supported by our finding that FGF receptor 1 ( FGFR 1 ) and heparan sulfate ( HS ) , known to be essential for FGF 2 signaling , were expressed by adult rat RGCs . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
JK GMS cells expressed high affinity FGF receptors ( FGFRs ) , and treatment with FGF 2 induced phosphorylation of FGFR 1 together with activation of extracellular signal regulated kinases ( ERK1 / ERK2 ) and c Jun N terminal kinase ( JNK ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using antibodies against fibroblast growth factor 2 ( FGF 2 ) , fibroblast growth factor receptor 1 ( FGFR 1 ) , FGFR 3 , bcl 2 , p 21 , parathyroid hormone related peptide ( PTHrP ) , and parathyroid hormone related peptide receptor ( PTHR 1 ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Pancreatic ductal adenocarcinomas ( PDACs ) overexpress several members of the fibroblast growth factor ( FGF ) family of ligands and the type 1 FGF receptor ( FGFR 1 ) , and enhanced FGF 2 protein levels correlate with shorter postoperative survival of patients with PDAC . ^^^ In this study , we investigated the effects of FGF 2 on cell proliferation and mitogen activated protein kinase ( MAPK ) activation before and after abrogation of FGFR 1 dependent signaling in 4 pancreatic cancer cell lines ( ASPC 1 , COLO 357 , MIA PaCa 2 , and PANC 1 ) . ^^^ Infection with the AdtrFGFR 1 virus resulted in abundant expression of the truncated FGFR 1 at the RNA and protein level , markedly attenuated FGF 2 induced proliferation in all 4 tested cell lines , and decreased FGF 2 dependent MAPK activation in the 3 cell lines in which FGF 2 activated this pathway . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
The following results were obtained : 1 ) EGF , TGFalpha , EGFR , PCNA , FGF 2 , pRb , and FGFR 1 4 were immunolocalized in the developing tissues . 2 ) Cytokine expression patterns indicated that the EGF family and FGF 2 essentially induced VL generation and cell proliferation . 3 ) FGFR 1 was diffusely localized in the primary epithelial band , but was strongly expressed in the E 12 14 VL and DL / enamel organ . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Here , we characterize FGF 2 , FGFR 1 , and heparin interactions using surface plasmon resonance ( SPR ) . ^^^ Interestingly , comparison of sensorgrams of sequential injections of FGF 2 and FGFR 1 and equimolar FGF 2 FGFR1 injections onto a heparin neoproteoglycan surface demonstrates that FGF 2 dramatically enhances the association of FGFR 1 with heparin and leads us to propose a model for the stepwise assembly of a ternary FGF FGFR HSPG complex . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
They mimicked FGF 1 and FGF 2 for receptor binding , signal transduction , proliferation of endothelial cells and differentiation of the bladder carcinoma cell NBT 2 which expresses FGFR 2b but not FGFR 1 . ^^^ The constitutive expression of FGFR 1 allowed binding of FGF 2 and AIdF 2 and inhibition of the proliferation of NBT 2 cells . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Coimmunoprecipitation and immunofluorescence studies demonstrated a direct specific association between alpha ( 5 ) beta ( 3 ) and FGF receptor 1 ( FGFR 1 ) in ECs and fibroblasts when exposed to both FGF 2 and fibrinogen but not with vitronectin . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
A monoclonal antibody to FGFR 1 also inhibited u PA mRNA expression induced by fibrinogen bound FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
CONCLUSION : FGF 2 was transferred to FGFR 1 through binding to HSPG , which is characteristically expressed on RASFs , resulting in RANKL and ICAM 1 mediated maturation of osteoclasts via ERK activation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Following ligand binding and subsequent internalization , both FGF 2 and FGFR 1 are translocated to the nucleus where they have activities distinct from those expressed at the cell surface . ^^^ In the studies reported in this paper , we examined the roles of clathrin dependent and independent endocytosis in the uptake of FGFR 1 and one of its ligands , FGF 2 . ^^^ While the uptake of FGF 2 occurred at least partly by a caveolar dependent mechanism , that of FGFR 1 was independent of both caveolae and coated pits . ^^^ Surprisingly , neither the uptake of FGF 2 nor FGFR 1 required the activity of the receptor tyrosine kinase . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Transcription profiling identified classes of genes that are differentially expressed in early and late retinal stem cells / progenitors in proliferating conditions and suggested that the distinct proliferative response to FGF 2 and EGF is likely due to differential expression of FGF receptor 1 ( FGFR 1 ) and EGF receptor ( EGFR ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To determine whether FGF 2 had an autocrine role , FGFR signaling was inhibited using recombinant adenovirus expressing dominant negative FGFR 1 ( RAdDN FGFR 1 ) and a specific inhibitor of FGFR 1 signaling , PD 166866 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Furthermore , anosmin 1 enhances FGF 2 signaling specifically through FGFR 1 IIIc in heterologous BaF 3 lymphoid cells in a heparan sulfate dependent manner . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
To determine the minimum size and sulfation content of active dermatan sulfate oligosaccharides , dermatan sulfate was first digested and then separated by size exclusion high pressure liquid chromatography , and the activity to facilitate FGF 2 and FGF 7 was assayed by the cellular proliferation of cell lines expressing FGFR 1 or FGFR 2 IIIb . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In contrast , initiated progenitors did not exit the cell cycle when stimulated with PDGF and / or FGF 2 . cDNA oligoarray analysis and RT PCR verification showed an early upregulation / induction of growth factor / receptors , PDGF A , PDGFR beta , IGFR 1 , IGF 1 and 2 , IL 6 , MEGF 5 , FRAG 1 , IRS 2 , HSPG , and FGFR 1 , followed by a late increase in the expression IGFBP 6 , PDGF alpha , FGFR 4A , c / ERB A , and FGFR 4 , 2 , and 1 during the tumorigenic progression . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Chronic obstructive pulmonary disease is associated with enhanced bronchial expression of FGF 1 , FGF 2 , and FGFR 1 . ^^^ In this study , the role of fibroblast growth factors ( FGF 1 and FGF 2 ) and their receptor , FGFR 1 , was assessed in bronchial airway wall remodelling in patients with COPD ( FEV 1 < 75 % ; n = 15 ) and without COPD ( FEV 1 > 85 % ; n = 16 ) . ^^^ Steady state mRNA levels of FGFR 1 were elevated in human ASM cells treated with either FGF 1 or FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In thyroids from three RAd treated animals , there was marked , significant inhibition of Tie 2 , FGFR 1 , VEGFR 1 , FGF 2 , and VEGF expression , compared with control goiters . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
With a treatment of FGF 2 , the expression of FGFR 1 slightly increased in the USC chondrocytes and was not related with the concentration of FGF 2 in the Ti chondrocytes . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RNA was extracted from posterior frontal ( PF ) and sagittal ( SAG ) sutures , and reverse transcription polymerase chain reaction ( RT PCR ) was performed for cranial suture candidate cytokines BMP 2 , BMP 3 , BMP 4 , FGF 2 , FGFR 1 , FGFR 2 , FGFR 4 , TGF betaRI , TGF betaRII , and TGF betaRIII . ^^^ There was a marked difference in FGFR 1 , FGF 2 , TGF betaRI , and TGF betaRII expression levels when comparing the fusing PF and nonfusing SAG sutures . ^^^ Although FGF 2 ligand expression was low , FGF receptor 1 ( FGFR 1 ) levels were markedly elevated with a bimodal expression pattern in both PF and SAG similar to that of BMP 2 , BMP 3 , and BMP 4 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
RESULTS : Seizures increase FGF 2 mRNA and protein levels in specific brain areas and upregulate the expression of its receptor FGFR 1 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Whereas FGF 2 , the prototypic ligand in a family of 22 members , activates all four tyrosine kinase FGF receptors ( FGFR 1 FGFR4 ) , other members demonstrate a higher degree of selectivity . ^^^ In addition , we demonstrate that , although activation of FGFR 1 by FGF 2 leads to proliferation of OL progenitors , it produces deleterious effects on differentiated OLs ( i . e . , aberrant reentry into cell cycle and down regulation of myelin proteins with a loss of myelin membrane ) . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Furthermore , up regulated phosphorylation of fibroblast growth factor receptor 1 ( FGFR 1 ) and Akt were observed in wild type Cbl cells upon FGF 2 stimulation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In liver samples , the expression of FGF 1 and FGF 2 and of FGF receptors ( FGFRs ; FGFR 1 , FGFR 2 ) was significantly elevated with time after bone marrow transplantation ( BMT ) compared with other factors , and co expression of GFP and FGFs or FGFRs could be detected . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Finally , axonal application of blocking antibody against the FGF receptor 1 ( FGFR 1 ) prevented the FGF 2 induced up regulation of BDNF and TrkB . ^^^ Our results suggest that FGF 2 acts on RGCs via FGFR 1 , activating the ERK pathway and CREB to increase BDNF synthesis , and PKA and CREB to increase TrkB synthesis . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
In the present study , we evaluated the expression of FGF 1 and FGF 2 as well as FGF receptors 1 4 ( FGFR 1 FGFR4 ) in 39 cases of Hodgkin ' s lymphoma , including all subtypes , as well as Hodgkin ' s lymphoma cell lines . ^^^ FGF 1 and FGF 2 and their receptors FGFR 2 FGFR4 , but not FGFR 1 , were found to be expressed by the malignant cells in the majority of cases . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
At 5 50 microM Abeta 1 40 reduced EC population , caused apoptosis , downregulated FGF 2 production , inhibited FGF 2 binding to heparin , and FGFR 1 phosphorylation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We examined the intracellular dynamics of FGF 2 , FGFR 1 and the HSPGs syndecan 2 and 3 , glypican 1 and 2 , and perlecan in neurones and glia in and around adult rat cerebral wounds . ^^^ In the intact cerebral cortex , FGF 2 and FGFR 1 mRNA and protein were constitutively expressed in astrocytes and neurones respectively . ^^^ After injury , expression of FGF 2 mRNA was up regulated only in astrocytes , whereas FGFR 1 mRNA expression was increased in both glia and neurones , a disparity indicating that FGF 2 may act as a paracrine and autocrine factor for neurones and glia respectively . ^^^ Specific HSPGs responded to injury by differentially co localizing with trafficked intracellular FGF 2 and FGFR 1 . ^^^ The spatiotemporal dynamics of FGF 2 FGFR 1 HSPG complex formation implies a role for individual HSPGs in regulating FGF 2 storage , nuclear trafficking and cell specific injury responses in CNS wounds . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Neutralizing FGFR 1 specific antibody abrogates the physiologic and chemoprotective effects of FGF 2 / FGFR1beta signaling and inhibits tumor growth in mice xenotransplanted with human AML . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
OBJECTIVE : To seek evidence of early vascular injury in the placental villous microcirculation in placental insufficiency identified by a high resistance umbilical Doppler study by examining for expression of fibroblast growth factor receptor 1 ( FGFR 1 ) , its transcription factor , early growth response factor 1 ( Egr 1 ) and plasma fibroblast growth factor 2 ( FGF 2 ) . ^^^ MAIN OUTCOME MEASURES : Microvascular endothelial cells expression of Egr 1mRNA , FGFR 1 mRNA and presence of soluble FGF 2 in fetal plasma . ^^^ The FGFR 1 activation and increase in FGF 2 in the fetal circulation are known to be very early features of the response of endothelium to injury . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We show that RGTA is comparable to heparin as it favors FGF 2 binding to FGFR 1 and FGF 2 dimerization and potentiates FGF 2 induced mitogenic activity . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
Hippocampal FGF 2 and FGFR 1 mRNA expression in major depression , schizophrenia and bipolar disorder . ^^^ We determined the distribution of hippocampal FGF 2 and its receptor ( FGFR 1 ) mRNA in post mortem brains of people who suffered from major depression , bipolar disorder and schizophrenia and those of controls . ^^^ METHODS : FGF 2 and FGFR 1 mRNA were measured within hippocampal CA 1 , CA 4 regions and the dentate gyrus ( DG ) , using in situ hybridization . ^^^ CONCLUSIONS : We found raised measures of FGFR 1 mRNA+ in major depression and , less so , in schizophrenia , along with reduced FGF 2 mRNA density in depression . ^^^ Perturbations of FGF regulation could be relevant to the pathogenesis of both disorders as FGF 2 and FGFR 1 are implicated in normal hippocampal synaptology , stem cell recruitment , and connectivity , and are modulated by corticosteroids . . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
We confirmed this by showing that all FLRTs can interact with FGFR 1 and FLRTs can be induced by the activation of FGF signalling by FGF 2 . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
FGF 2 inhibition of OPC differentiation was effectively blocked by siRNA targeted to FGFR 1 , but not FGFR 2 or FGFR 3 . ^^^ We propose a model of direct FGF 2 activation of FGFR 1 leading to inhibition of OPC differentiation . ^^^
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
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Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA
Interacting proteins: P09038 and P11362	Pubmed	SVM Score :0.0
NA