| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| In two cell lines , HS 1 derived from ALL carrying t ( 8 ; 14 ) and FR 4 derived from a plasmacytoma carrying a complex form of t ( 8 ; 14 ) , the signal of Ig gamma was observed at the breakpoint region 8q24 . 1 of the der ( 8 ) in addition to the der ( 14 ) , indicating that translocation event occurred within the Ig gamma locus . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| Induction of tyrosine phosphorylation on a variety of cellular proteins , such as Vav , Cbl , and HS 1 , upon BCR cross linking was also abolished in these B cells . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| The binding of antigen to the B cell antigen receptor ( BCR ) results in the activation of protein tyrosine kinases ( PTKs ) such as Lyn and Syk , and the phosphorylation of several substrate proteins including HS 1 and SLP 65 . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| To investigate abnormalities in BCR mediated signaling pathway in lupus B lymphocytes , we analyzed HS 1 , a molecule downstream of BCR , in 80 Japanese SLE patients . ^^^ WEHI 231 cell , a mouse B cell line , transfected with the aberrant HS 1 displayed a significantly increased cell death upon cross linking of BCR . ^^^ These data suggest that the aberrant HS 1 molecule may transmit an accelerated signal after BCR stimulation and may play a role in the activation of autoreactive B lymphocytes . . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| WEHI 231 cells transfected with EPEP+ HS 1 were 100 fold more sensitive to B cell receptor ( BCR ) mediated apoptosis than were those transfected with HS 1 without EPEP . ^^^ CONCLUSION : These results suggest that HS 1 with the EPEP insertion polymorphism transmits accelerated signals from BCR and is involved in the pathogenesis of SLE . . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| Hematopoietic lineage cell specific protein 1 ( HS 1 ) is an F actin and actin related proteins 2 and 3 ( Arp2 / 3 ) binding protein that undergoes a rapid tyrosine phosphorylation upon B cell antigen receptor ( BCR ) activation . ^^^ Density gradient centrifugation of Triton 10 100 lysates from B lymphocytes demonstrated that HS 1 was translocated in response to BCR cross linking into lipid raft microdomain along with Arp2 / 3 complex and Wiskott Aldrich syndrome protein . ^^^ HS 1 green fluorescent protein was localized in membrane patches enriched with GM 1 gangliosides and BCR in the cells treated with anti IgM antibody . ^^^ Colocalization of HS 1 green fluorescent protein with BCR was also correlated with tyrosine phosphorylation of HS 1 . ^^^ Interestingly a murine HS 1 mutant at the tyrosine residues Tyr 388 and Tyr 405 targeted by Syk failed to respond to BCR cross linking for either translocation into lipid rafts or colocalization with BCR within cells . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| As HS 1 is a protein pivotal in the signal cascade triggered by B cell receptor ( BCR ) stimulation , we studied its pattern of expression following BCR engagement . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| The 75 kD HS 1 protein is highly tyrosine phosphorylated during B cell antigen receptor ( BCR ) mediated signaling . ^^^ Owing to low expression of HS 1 , WEHI 231 derived M 1 cells , unlike the parental cells , are insensitive to BCR mediated apoptosis . ^^^ Here , we show that BCR associated tyrosine kinases Lyn and Syk synergistically phosphorylate HS 1 , and that Tyr 378 and Tyr 397 of HS 1 are the critical residues for its BCR induced phosphorylation . ^^^ Thus , tyrosine phosphorylation of HS 1 is required for BCR induced apoptosis and nuclear translocation of HS 1 may be a prerequisite for B cell apoptosis . . ^^^ |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P31946 |
Pubmed |
SVM Score :0.0 |
| NA |
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