| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| In this report we show that Shc proteins become tyrosine phosphorylated after BCR ligation on both transformed and normal human B cells . ^^^ Co ligation of the BCR with the CD 19 molecule results in a marked increase of these events , whereas CD 19 cross linking alone does not induce Shc tyrosine phosphorylation or translocation . ^^^ Thus , in B cells the Shc complex may represent a molecular junction between the BCR and the mitogenic p21ras cascade . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Our results indicate that the SH 2 domain of BCR ABL is not required for the induction of growth factor independence and is not required for the association of BCR ABL with rasGAP or SHC . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Shc protein is tyrosine phosphorylated upon B cell receptor ( BCR ) activation and after its phosphorylation interacts with the adaptor protein Grb 2 . ^^^ Tyrosine phosphorylation of Shc and its association with Grb 2 were profoundly reduced in both Lyn and Syk deficient B cells upon BCR stimulation . ^^^ Because Lyn is required for activation of Syk upon receptor stimulation , our results suggest that the Lyn activated Syk phosphorylates Shc during BCR signaling . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylated Shc and p 145 coprecipitated with anti Shc antibodies following B cell antigen receptor ( BCR ) cross linking or interleukin 4 ( IL 4 ) receptor activation in B cells , and after lipopolysaccharide ( LPS ) treatment or IgG Fc receptor ( Fc gamma R ) cross linking in macrophages . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| In BCR ABL delta 1 40 reactivated by the SH 3 deletion , transphosphoryation of other cellular proteins like p 62 or SHC in vivo and autophosphorylation with recruitment of GRB 2 were also recovered . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| The BCR activates Ras , presumably by the formation of a Shc Grb 2 adaptor complex , which recruits the Grb 2 associated guanine nucleotide exchange factor Sos to the plasma membrane . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Syk expression reconstituted several signaling events upon anti IgM stimulation , including Syk phosphorylation and association with the BCR , tyrosine phosphorylation of numerous proteins including Shc , and activation of mitogen activated protein kinase . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| In Rat 1 cells expressing GST / Bcr Abl ( Delta 1 160 ) , phosphotyrosine contents of p 62 and Shc were 70 % decreased . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| B cell antigen receptor ( BCR ) stimulation induces tyrosine phosphorylation of the Shc adaptor protein and its association with Grb 2 . ^^^ We reveal here an additional complexity of the BCR induced Shc / Grb2 complex : it contains tyrosine phosphorylated proteins of 130 , 110 and 75 kDa . ^^^ Cbl constitutively interacts with the SH 3 domains of Grb 2 , with a preference for the amino terminal domain , and is in this way recruited to Shc upon BCR stimulation . ^^^ Immunodepletion studies showed that Grb 2 associated Cbl can be phosphorylated by BCR induced tyrosine kinases independent of a Shc / Grb2 interaction . ^^^ This indicates that the BCR can also couple to a Grb 2 complex without the involvement of Shc . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| The results reported here indicate that the resting , nonphosphorylated BCR associates with the Grb 2 / Sos linker SHC via the Ig alpha immunoreceptor based tyrosine activation motif ( ITAM ) . ^^^ In view of previous studies which demonstrated p21ras co capping with ligated BCR , the data presented here suggest that Ig alpha / beta and SHC tyrosine phosphorylation dependent recruitment of the Grb2 / Sos complex to the receptor can occur and may provide a mechanism by which the nucleotide exchange activity of Sos could mediate activation of BCR localized p21ras . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Stimulation of the B cell Ag receptor ( BCR ) has been reported to induce tyrosine phosphorylation of a 145 kDa protein and its association with the adapter protein Shc . ^^^ Further analysis revealed that both maximal phosphorylation of SHIP and its association with Shc require co clustering with the Fc receptor for IgG ( Fc gamma RII ) rather than stimulation of the BCR alone . ^^^ Since co clustering of the BCR and Fc gamma RII also down regulates proliferation induced by Ag receptor stimulation , we hypothesize that tyrosine phosphorylation of SHIP and its association with Shc contribute to negative signaling through effects on inositol and phosphatidylinositol metabolism . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Analysis of specific effector molecules revealed that tyrosine phosphorylation of Shc , but not rasGAP or Vav , correlated with the unique ability of BCR ligation to trigger p21ras activation in CD45+ cells . ^^^ BCR mediated Shc phosphorylation and recruitment of Grb 2 depended on CD 45 expression . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| APS bound to Shc irrespective of stimulation and bound to Grb 2 after stimulation , suggesting that it plays a role in linkage from BCR to Shc / Grb2 pathway . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| The major target proteins have been identified , and are very similar to the known substrates of BCR / ABL , including Shc , CBL , CRKL , and several proteins in the cytoskeleton . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate that the BCR induced ERK activation is reduced by loss of Grb 2 or expression of a dominant negative form of Ras , RasN 17 , whereas this response is not affected by loss of Shc . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Indeed , it has been proposed that SHIP serves as a linking molecule for the regulation of the extracellular signal regulated kinase pathway in BCR signaling , because SHIP associates with Shc . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| BASH becomes tyrosine phosphorylated with the B cell Ag receptor ( BCR ) cross link or by coexpression with Syk and Lyn and associates with signaling molecules including Syk and a putative chicken Shc homologue . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| We and others have reported that SHIP is rapidly tyrosine phosphorylated upon B cell antigen receptor ( BCR ) cross linking and forms a complex with the adapter protein Shc . ^^^ Here , we report that cross linking of the BCR induces association between Grb 2 and SHIP as well as association between Shc and SHIP . ^^^ Shc and Grb 2 may help to localize SHIP to the cell membrane , regulating SHIP ' s inhibitory function following BCR stimulation . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| SHIP 2 shows a maximal tyrosine phosphorylation and association to Shc after ligation of BCR to FcgammaRIIB but not after stimulation of BCR alone . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Here we describe that the cell membrane associated Shc is partially dephosphorylated in BCR Fc gammaRIIb 1 co ligated samples , suggesting that its function in regulating p21ras monomeric G protein is impaired . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| We and others have shown that the Bcr Abl oncoprotein binds activators of the Ras pathway such as Grb 2 and Shc . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Cross linking of the B cell antigen receptor ( BCR ) induces tyrosine phosphorylation of Shc , which is believed to lead to the activation of Ras . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of Shc in response to B cell Ag receptor ( BCR ) engagement creates binding sites for the Src homology 2 ( SH 2 ) domain of Grb 2 . ^^^ Given the importance of Shc phosphorylation , we investigated the mechanism by which the BCR stimulates this response . ^^^ We found that both the SH 2 domain and phosphotyrosine binding ( PTB ) domain of Shc are important for BCR induced tyrosine phosphorylation of Shc and the subsequent binding of Grb 2 to Shc . ^^^ Because the major ligand for the Shc PTB domain is SHIP , we asked whether the interaction of Shc with SHIP was required for BCR induced tyrosine phosphorylation of Shc . ^^^ Using SHIP deficient DT 40 cells , we show that SHIP is necessary for the BCR to induce significant levels of Shc tyrosine phosphorylation . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| Moreover , we demonstrate that addition of both linker for activation of T cells ( LAT ) and Grb 2 related adaptor downstream of Shc ( Gads ) to SLP 76 allow SLP 76 to be recruited into GEMs , whereby the BCR function is reconstituted . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| When pre BCR was cross linked with anti micro heavy chain antibodies , significant phosphorylation of intracellular molecules , including Syk , Shc , ERK MAP kinase , and AKT , and an activation of Ras were observed without regard to deficiency of BLNK expression , suggesting that BLNK is not required for pre BCR mediated activation of MAP kinase and phosphatidyl inositol 3 ( PI 3 ) kinase signalling . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| We investigated the expression and phosphorylation of Src homology 2 and collagen homology domains ( SHC ) [ corrected ] proteins in subpopulations of CML primary cells . ^^^ Shc polypeptides are tyrosine kinase substrates that are constitutively tyrosine phosphorylated in continuous cell lines of CML origin . ^^^ High levels of Shc expression were found in the CD34+ cells from CML BC , CML CP and normal bone marrow . ^^^ Shc proteins were constitutively phosphorylated in the CD34+ fractions from CML cells ( both CP and BC ) , but not in normal CD34+ cells . ^^^ These data bear implications for the role of Shc in normal hemopoiesis and CML leukemogenesis : ( a ) dramatic changes of Shc expression during terminal differentiation of hemopoietic cells adds a further level of regulation to the signal transduction function of Shc ; and ( b ) constitutive Shc tyrosine phosphorylation in the rare CD34+ cells of CML CP might contribute to the selection of this subpopulation during the blast crisis transformation of CMLs . . ^^^ |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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