| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.67907953 |
| Additional studies of unstimulated CH 12 and wild type splenic B cells revealed the constitutive association of PTP1C with the resting BCR complex , as evidenced by coprecipitation of PTP1C protein and phosphatase activity with BCR components and the depletion of BCR associated tyrosine phosphatase activity by anti PTP1C antibodies . 0.67907953^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.99433898 |
| The present study shows that SHP 1 is constitutively associated with the BCR in B 1 cells . 0.99433898^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.53730731 |
| CD 5 promotes a prolonged interaction between BCR and SHP 1 , which may be inhibitory to BCR signaling . 0.53730731^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| More recently , SHP 1 has been shown to negatively regulate B cell antigen receptor ( BCR ) initiated signaling . ^^^ To elucidate potential mechanisms of SHP 1 action in BCR signal transduction , we studied proteins that interact with SHP 1 in B cells . ^^^ However , significant tyrosine phosphorylation of SLP 76 as well as of SHP 1 was observed after BCR ligation . ^^^ Collectively , these results suggest possible mechanisms by which SHP 1 may modulate signals delivered by BCR engagement . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| CD 22 can interact with both the B cell antigen receptor ( BCR ) complex and signalling molecules , including the protein tyrosine phosphatase SHP 1 ( PTP1C , SHP ) , a putative negative regulator of BCR signalling . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| These motifs are phosphorylated upon BCR crosslinking , and can bind the tyrosine phosphatase SHP 1 , a putative negative regulator of signalling from the BCR . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Loss of heterozygosity with microsatellite markers around the SHP 1 gene was detected in 79 % of informative ALL cases . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| RT PCR showed that all samples expressed HCP gene , only one missense mutation at codon 225 ( AAC to AGC , Asn to Ser ) within N terminal SH 2 domain was found in an ALL patient . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| In view of recent data implicating the cytosolic phosphotyrosine phosphatase PTP1C in the regulation of BCR signaling capacity , we used W delta cells to investigate the potential role for PTP1C in modulating the cell response to BCR activation . ^^^ The association of PTP1C with this 44 kDa phosphoprotein following mIgM cross linking was also observed in two additional B cell lines representing an immature state of differentiation , but was not detected after BCR engagement in two representative mature B cell lines or in splenic B cells . ^^^ While the structural and biochemical properties of pp 44 require further definition , the findings suggest that BCR triggered interactions of PTP1C with pp 44 occur only in the context of an immature state of cellular differentiation and the induction of apoptosis . ^^^ These data therefore suggest that PTP1C interactions with pp 44 may be relevant to the transduction of BCR signals which evoke cell death . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Here it was shown that the Fc gamma RIIB 1 recruits the phosphotyrosine phosphatase PTP1C after BCR coligation . ^^^ Thus , PTP1C is an effector of BCR Fc gamma RIIB 1 negative signal cooperativity . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Complexes of either CD22 / PTP 1C / Syk / PLC gamma ( 1 ) could be isolated from B cells stimulated by BCR engagement or a mixture of hydrogen peroxidase and sodium orthovanadate , respectively . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Another inhibitory receptor , the low affinity Fc receptor for immunoglobulin G ( IgG ) ( Fc gamma RIIb 1 ) , has been shown to bind SHP 1 when cocross linked with the antigen receptor on B cells ( BCR ) . ^^^ However , coligation of Fc gamma RIIb 1 with BCR and with Fc epsilon RI on mast cells leads to recruitment of the inositol 5 ' phosphatase SHIP and to inhibition of mast cells from SHP 1 deficient mice . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| The cytosolic SHP 1 and transmembrane CD 45 protein tyrosine phosphatases ( PTP ) play critical roles in regulating signal transduction via the B cell antigen receptor ( BCR ) . ^^^ For example , BCR mediated mitogenesis is essentially ablated in mice lacking CD 45 ( CD 45 ( ) ) , but is enhanced in SHP 1 deficient motheaten ( me ) and viable motheaten ( mev ) mice . ^^^ To determine whether these PTPs act independently or coordinately in modulating the physiologic outcome of BCR engagement , we assessed B cell development and signaling in CD 45 deficient mev ( CD 45 / SHP 1 ) mice . ^^^ Here we report that the CD 45 / SHP 1 ) cells undergo appropriate induction of protein kinase activity , mitogen activated protein kinase activation , and proliferative responses after BCR aggregation . ^^^ However , BCR elicited increases in the tyrosine phosphorylation of several SHP 1 associated phosphoproteins , including CD 19 , were substantially enhanced in CD 45 / SHP 1 , compared to wild type and CD 45 cells . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Hypophosphorylation on tyrosine residues of these molecules resulted in failure of recruiting the tyrosine phosphatase SHP 1 and inositol phosphatase SHIP , SH 2 containing potent inhibitors of BCR induced B cell activation , to the coreceptors . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Polygenic autoimmune traits : Lyn , CD 22 , and SHP 1 are limiting elements of a biochemical pathway regulating BCR signaling and selection . ^^^ As in invertebrates , genetic effects of loci with only one functional allele can be used to analyze signaling networks in mice , demonstrating that negative regulation of the BCR is a complex quantitative trait in which Lyn , the coreceptor CD 22 , and the tyrosine phosphatase SHP 1 are each limiting elements . ^^^ The biochemical basis of this complex trait involves a pathway requiring Lyn to phosphorylate CD 22 and recruit SHP 1 to the CD22 / BCR complex . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Moreover , cross linking of B cell Ag receptor ( BCR ) enhances both tyrosine phosphorylation of CD 72 and association of CD 72 with SHP 1 in B cell line WEHI 231 . ^^^ These results indicate that CD 72 recruits SHP 1 upon tyrosine phosphorylation induced by BCR signaling , suggesting that CD 72 is a negative regulator of BCR signaling . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| However , coligation of Fc gamma RIIB 1 with B cell Ag receptors ( BCR ) inhibits BCR mediated signaling by a mechanism that may involve recruitment of phosphatases SHP 1 , SHP 2 , and the SH 2 containing inositol 5 ' phosphatase ( SHIP ) to the phosphorylated Fc gamma RIIB 1 immunoreceptor tyrosine based inhibitory motif . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| The protein tyrosine phosphatase SHP 1 is known to regulate the strength of BCR signaling . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| In Ag specific Id positive B cells , the co cross linking of BCR and Fc gamma RIIB 1 by anti Id Ab resulted in the association of both Src homology 2 containing protein tyrosine phosphatase ( SHP 1 ) and Src homology 2 containing inositol phosphatase ( SHIP ) with the Fc gamma RIIB 1 ; however , only SHIP activity was detected . `` Superclustering ' ' of the BCR and Fc gamma RIIB 1 complex induced by stimulation with anti Id Ab plus polyvalent Ag synergistically activated SHP 1 . ^^^ The degree of co cross linking between BCR and Fc gamma RIIB 1 may determine the activation status of SHP 1 and SHIP . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Paired immunoglobulin like receptor B ( PIR B ) inhibits BCR induced activation of Syk and Btk by SHP 1 . ^^^ Based on these results , we propose a model of PIR B mediated inhibitory signaling in which coligation of PIR B and BCR results in phosphorylation of ITIMs by Lyn , subsequent recruitment of SHP 1 , and a resulting inhibition of the BCR induced inositol 1 , 4 , 5 trisphosphate generation by dephosphorylation of Syk and Btk . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Src homology 2 domain bearing tyrosine phosphatase SHP 1 and Src homology 2 domain bearing inositol 5 ' phosphatase SHIP usually participate in negative regulation of the BCR . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylated CD 22 recruits numerous cytoplasmic effector molecules including SHP 1 , a potent phosphotyrosine phosphatase that down regulates B cell antigen receptor ( BCR ) and CD 19 generated signals . ^^^ These data demonstrate that CD 22 functions as a molecular `` scaffold ' ' that specifically coordinates the docking of multiple effector molecules , in addition to SHP 1 , in a context necessary for BCR dependent SHIP activity and JNK stimulation . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| It has been reported that BCR mediated signaling in B 1 cells is negatively regulated by signals from CD 22 , CD 5 and CD 72 co receptors , and that Lyn kinase plays a crucial role in tyrosine phosphorylation of immunoreceptor tyrosine based inhibitory motifs on the CD 22 and CD 72 , which recruits SHP 1 to the BCR complex . ^^^ We found that Lyn kinase is also essential for the tyrosine phosphorylation of CD 5 and subsequent recruitment of SHP 1 in B 1 cells upon cross linking of BCR . ^^^ Ly 49 was rapidly tyrosine phosphorylated upon cross linking of BCR and SHP 1 was found to recruit to the phosphorylated Ly 49 . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Although CD 22 contains three immunoreceptor tyrosine based inhibitory motifs ( ITIMs ) , only the two carboxyl terminal ITIM tyrosines are required for efficient recruitment of the SHP 1 phosphatase after BCR ligation . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Although simultaneous CD 19 and BCR engagement significantly enhanced CD19 / Lyn complex formation and [ Ca ( 2+ ) ] ( 1 ) responses , downstream tyrosine phosphorylation of CD 22 and multiple other cellular proteins was inhibited , as was SHP 1 recruitment to phosphorylated CD 22 . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Several proteins ( CD 19 , CD 22 , SHP 1 , and Dok ) and a lipid raft marker were co recruited to the BCR , regardless of the presence or absence of FcgammaRII and SHIP . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| We previously showed that B cell linker protein ( BLNK ) is a physiological substrate of SHP 1 and that B cell receptor ( BCR ) induced activation of c Jun NH ( 2 ) terminal kinase ( JNK ) is significantly enhanced in cells expressing a form of SHP 1 lacking phosphatase activity ( SHP 1 C / S ) . ^^^ Finally , BCR induced apoptosis was suppressed in SHP 1 C / S expressing cells and coexpression of Nck SH 2 mutants or a dominant negative form of SEK 1 reversed this phenotype . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| This autoimmunity may result from loss of a feedback inhibitory pathway in which Lyn phosphorylates CD 22 , triggering recruitment of the tyrosine phosphatase SHP 1 to the plasma membrane , which then dampens BCR signaling . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Lyn ( up / up ) B cells are characterized by the constitutive phosphorylation of negative regulators of B cell antigen receptor ( BCR ) signaling including CD 22 , SHP 1 , and SHIP 1 , and display attributes of lymphocytes rendered tolerant by constitutive engagement of the antigen receptor . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Src homology protein 1 ( SHP 1 ) plays an important role in B cell Ag receptor ( BCR ) differentiation , proliferation , survival , and apoptosis . ^^^ After BCR stimulation in apoptotic cells , SHP 1 has been shown to be recruited to phosphorylated immunoreceptor tyrosine based inhibitory motifs present in receptors such as CD 22 and CD 72 . ^^^ Furthermore , more SHP 1 molecules translocate into lipid rafts , and their association with actin was increased after BCR stimulation . ^^^ Therefore , actin dephosphorylation by SHP 1 is essential for actin depolymerization after BCR stimulation . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| For example , after BCR stimulation in apoptotic B cells , SHP 1 has been shown to be recruited to phosphorylated ITIMs present in receptors such as CD 72 . ^^^ The results suggest that SHP 1 plays a critical role in the reorganization of cytoskeletal architecture mediated via BCR stimulation . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| We found that SLP65 / BLNK reaches maximal tyrosine phosphorylation at earlier time points in CD 22 ( / ) than in wild type B cells upon BCR cross linking , suggesting that SLP65 / BLNK is a substrate of SHP 1 . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Mounting evidence indicates that abnormal BcR signaling , such as occurs in SHP 1 and Lyn deficient mice , results in production of pathogenic autoantibodies and lupus like glomerulonephritis , suggesting that altered signaling thresholds could underlie the development of systemic autoimmunity . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| SHP 1 remained attached to the BCR in subgroup Ia , but not in subgroup Ib , where signal transduction was associated with an excess of truncated CD79b . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Interaction of B cells with membrane antigen results in the formation of the B cell synapse : the B cell receptor ( BCR ) and antigen concentrate in the contact zone while CD45 / B220 and the phosphatase SHP 1 are excluded . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Given that SHP 1 binds to CD 22 upon BCR ligation , our findings suggest that dephosphorylation of SLP 76 recruited to CD 22 by SHP 1 inhibits BCR induced JNK activation , dictating apoptosis . . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| Activation of these inhibitory co receptors occurs by phosphorylation on their cytoplasmic inhibitory immunoreceptor tyrosine based inhibition motifs ( ITIMs ) , followed by recruitment of the tyrosine phosphatase SHP 1 or the lipid phosphatase SHIP , and depends on their association with the BCR . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that SHIP is necessary for human FcgammaRIIB to inhibit BCR signaling , and can not be replaced by SHP 1 or SHP 2 . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| We therefore studied mRNA expression of HCP in pure populations of CD34+ cells , granulocytes , platelets and lymphocytes from patients with PV , chronic myeloid leukaemia ( CML ) or essential thrombocythemia ( ET ) , as well as healthy controls . ^^^ Moreover , HCP mRNA expression was similar in ET and CML compared to controls . ^^^ |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11274 and P29350 |
Pubmed |
SVM Score :0.0 |
| NA |
|