| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :1.1719321 |
| An in vitro translated CAR acting as a retinoid 10 receptor alpha heterodimer binds directly to the two nuclear receptor sites NR 1 and NR 2 within PBREM . 1.1719321^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.57939335 |
| The nuclear orphan receptor constitutive active receptor ( CAR ) can be activated to induce CYP2B genes by the potent phenobarbital type inducer 1 , 4 bis [ 2 ( 3 , 5 dichloropyridyloxy ) ] benzene ( TCPOBOP ) in which the receptor forms a heterodimer with the retinoid 10 receptor ( RXR ) and binds to a conserved enhancer element NR 1 . 0.57939335^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The primary structure showed a high homology with the metabotropic glutamate receptors ( mGluR ) , and a limited homology with the Ca2+ / polyvalent cation receptors ( CaR ) . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| GDNF also protected striatal CaR positive neurons against glutamate toxicity . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| In vitro translated CAR bound to NR 1 , but only in the presence of similarly prepared RXR . ^^^ PBREM was synergistically activated by transfection of CAR and RXR in HepG 2 and HEK 293 cells when the NR 1 site was functional . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The calcium sensing receptor ( CaR ) is a G protein coupled receptor that displays 19 25 % sequence identity to the gamma aminobutyric acid type B ( GABAB ) and metabotropic glutamate ( mGlu ) receptors . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| In addition , the role of dopamine ( DA ) D 2 , serotonin ( 5 HT ) 2A / 2C , alpha 1 , 5 HT1A , DA D 4 , muscarinic and glutamate receptors in the suppression of CAR induced by various classes of drugs is evaluated . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Structurally , the CaR is a novel member of a growing G protein coupled receptor superfamily , which includes metabotropic glutamate receptors ( mGluRs ) [ 1 ] , [ gamma ] aminoisobutyric acid ( GABA B ) receptors [ 2 ] and vomeronasal organ receptors [ 3 ] . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| This constitutive activity was not diminished when the mutations were transferred to a chimeric receptor Ca / 1a consisting of the amino terminal domain of the CaR and the 7 transmembrane and intracellular domains of the metabotropic glutamate receptor mGluR1a . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| CAR binds to retinoic acid beta 2 response elements ( betaRARE ) and to the NR 1 and NR 2 sites of the PBRU and activates transcription of reporter genes in cell lines . ^^^ However , conversion of NR 1 into betaRARE was the equivalent of an inactivating mutation , indicating that CAR does not activate PB dependent transcription via NR 1 in the natural sequence context . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The nuclear orphan receptor CAR ( constitutively active receptor or constitutive androstane receptor ) can be activated in response to xenochemical exposure , such as activation by phenobarbital of a response element called NR 1 found in the CYP2B gene . ^^^ Here various steroids were screened for potential endogenous chemicals that may activate CAR , using the NR 1 enhancer and Cyp2b10 induction in transfected HepG 2 cell and / or in mouse primary hepatocytes as the experimental criteria . 17beta Estradiol and estrone activated NR 1 , whereas estriol , estetrol , estradiol sulfate , and the synthetic estrogen diethylstilbestrol did not . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| We also made use of truncation and deletion mutants of the CaR and CaR / metabotropic glutamate receptor type 1 ( mGluR 1 ) chimeras to study both the site of action of R 568 and the effect of the Leu125Pro activating mutation . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| CAR is a member of the nuclear receptor family ( NR 1 ) mostly expressed in the liver , which heterodimerizes with retinoid 10 receptor ( RXR ) and was shown to transactivate both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The nuclear receptor 1 ( NR 1 ) site of the PBRE is sufficient for CAR mediated transactivation , but additional sequences within the PBRE , and hence the proteins that bind to them , are required for the interaction of CAR with SRC 1 . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Calcium sensing ( CaR ) and Group 1 metabotropic glutamate receptors exhibit overlapping expression patterns in brain , and share common signal transduction pathways . ^^^ To determine whether CaR and Group 1 metabotropic glutamate receptors ( mGluRs ) ( mGluR1alpha and mGluR 5 ) can form heterodimers , we immunoprecipitated CaR from bovine brain and observed co precipitation of mGluR1alpha . ^^^ CaR becomes sensitive to glutamate mediated internalization when present in CaR : mGluR1alpha heterodimers . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| In this study , we examined whether conserved amino acid residues involved in the binding of glutamate to metabotropic glutamate receptors ( mGluRs ) also participate in the potentiation of the activity of CaR by l phenylalanine . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| The majority of binding and hCAR activation is derived from the NR 1 portion of the CAR RE . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Purified constitutive androstane receptor ( CAR ) / retinoid 10 receptor ( RXR ) bound to the core NR 1 and NR 2 sites and to a third NR 3 site to the 5 ' side of the core in Cyp2b10 . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Metabotropic glutamate receptors ( mGluRs ) and calcium receptors ( CaR ) are closely related G protein coupled receptors ( GPCRs ) . ^^^ The similar structural and functional properties of mGluRs and CaRs include conserved amino acid residues involved in glutamate binding in mGluRs and Ca2+ binding in the CaR . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| These traits , along with a significant homology to the human calcium sensing receptor ( CaR , 34 % aa sequence identity ) , the taste receptor 1 ( T1R1 , 28 % ) , and the metabotropic glutamate receptor 1 ( mGluR 1 , 24 % ) , places GPRC6A in family C of the GPCRs . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Second , two CaR mGlu chimeric receptor constructs that retained the VFT domain of CaR , one containing the extracellular Cys rich region of CaR and the other containing the Cys rich region of the rat metabotropic glutamate type 1 ( mGlu 1 ) receptor , together with the rat mGlu 1 transmembrane region and C terminal tail , retained amino acid sensing . ^^^ The findings support the concept that the mGlu 1 amino acid binding site for L glutamate is conserved as an L amino acid binding site in its homolog , the CaR . . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Constitutive androstane receptor ( CAR ) RXR ( retinoic 10 receptor ) heterodimers are known to bind to NR 1 , NR 2 and NR 3 . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| To gain an insight into the long term molecular changes involved in the above described long term nociceptive effects of neonatal noxious insult at the spinal level , we performed DNA microarray analysis ( using microarrays containing oligo probes for 205 genes encoding receptors and transporters for glutamate , GABA , and amine neurotransmitters , precursors and receptors for neuropeptides , and neurotrophins , cytokines and their receptors ) to compare gene expression profiles in the lumbar spinal dorsal horn ( LDH ) of adult ( P 60 ) male rats that received neonatal CAR treatment within ( at postnatal day 3 ; P 3 ) and outside ( at postnatal 12 ; P 12 ) of the sensitive period . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| TSO increased CAR nuclear localization in mouse liver , activated the human Cyp2B6 promoter in liver in vivo , and activated a reporter plasmid that contains five nuclear receptor 1 ( NR 1 ) binding sites in HepG 2 cells . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Because constitutively activated receptor ( CAR ) is a major transcription factor driving the xenobiotic induced stimulation of CYP2B1 / 2 through NR 1 , the role of CAR as a preferential mediator of DAS effect is discussed . . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| Mutation of NR 3 resulted in similar effects on transactivation of the PBRU by CAR in HepG 2 cells as did mutations of NR 1 and NR 2 . ^^^ In the linear sequence NR 3 is further from NR 1 than NR 2 is , but in a nucleosomal structure , NR 3 is well positioned for cooperative recruitment of GRIP1 / SRC 2 by CAR / RXR that is bound to NR 3 and either NR 1 or NR 2 , while NR 1 and NR 2 are on opposite sides of the nucleosome separated by the histone core . ^^^ These results demonstrate that NR 3 is functionally similar to NR 1 and NR 2 in CAR transactivation of the PBRU in vitro and suggest that NR 3 may have a greater role in a chromatin context in vivo than is apparent from transient transfection studies . . ^^^ |
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| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| In mammals , the CNC family members nuclear factor erythroid 2 p 45 related factors 1 and 2 ( Nrf 1 and Nrf 2 ) have been shown to be involved in the transcriptional up regulation of cytoprotective genes including those encoding glutamate cysteine ligase , NAD ( P ) H : quinone oxidoreductase , glutathione S transferases and aldo keto reductases . ^^^ |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and Q05586 |
Pubmed |
SVM Score :0.0 |
| NA |
|