| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| METHODS : The presence of the high affinity Coxsackievirus and adenovirus receptor ( CAR ) for adenovirus type 5 , as well as endothelial growth factor receptor ( EGFR ) and alpha , integrins ( ITGAVs ) , were analyzed in primary tumors by using immunohistochemical studies and in primary meningioma cell cultures by using fluorescence activated cell sorting . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| When signaling through the epidermal growth factor receptor was inhibited in T 4 2 cells , cells reverted to a normal phenotype , CAR protein expression was significantly reduced , and the protein relocalized to cell cell junctions . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| This EGFR targeted CRAd exhibited increased infection efficiency and oncolytic replication on CAR deficient cancer cells and augmented lateral spread in CAR deficient 3 D tumor spheroids in vitro . ^^^ When compared to its parent control with native tropism , the new CRAd exhibited similar cytotoxicity on CAR positive cancer cells , but up to 1000 fold enhanced oncolytic potency on CAR deficient , EGFR positive cancer cells . ^^^ In addition , EGFR targeted CRAd killed primary human CAR deficient brain tumor specimens that were refractory to the parent control virus . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| The current experiments demonstrate that this occurs because CaR activation `` transactivates ' ' the EGF receptor ( EGFR ) . ^^^ Time dependent increases in tyrosine phosphorylation of the EGFR after addition of extracellular calcium ( [ Ca2+ ] o , 3 mM ) occurred in stably CaR transfected HEK 293 cells but not in non transfected HEK 293 cells . ^^^ AG 1478 , an EGFR kinase inhibitor , prevented the CaR mediated increases of pERK and PTHrP release , while AG 1296 , a PDGFR kinase inhibitor , had no effect . ^^^ Inhibitors of matrix metalloproteinase and heparin bound EGF prevented the CaR mediated increases of pERK and PTHrP , consistent with a `` triple membrane spanning signaling ' ' requirement for transactivation of the EGFR by the CaR . ^^^ Proximal and distal signal transduction cascades activated by the CaR may reflect transactivation of the EGFR by the extracellular calcium sensing receptor . . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| Because the CaR is a G protein coupled receptor ( GPCR ) , it is likely to transactivate the epidermal growth factor receptor ( EGFR ) or the platelet derived growth factor receptor ( PDGFR ) . ^^^ In this study , we hypothesized that activation of the CaR transactivates the EGFR or PDGFR , and examined whether transactivation affects PTHrP secretion in PC 3 human prostate cancer cells . ^^^ Taken together , these findings indicate that activation of the CaR transactivates the EGFR , but not the PDGFR , leading to phosphorylation of ERK1 / 2 and resultant PTHrP secretion , although CaR EGFR ERK might not be the only signaling pathway for PTHrP secretion . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| They transduced human carcinoma cells independently of the CAR pathway , via cell surface receptors targeted by specific monoclonal antibodies , that is , EGF R on A 549 , HT 29 and SW 1116 , HER 2 / neu on SK OV 3 and SK BR 3 , CA 242 ( epitope recognized by the monoclonal antibody C 242 ) antigen on HT 29 and SW 1116 , and PSMA ( prostate specific membrane antigen ) expressed on HEK 293 cells , respectively . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| Derivative AdDelta 24 425S11 infects cells deficient in coxsackie / adenovirus receptor ( CAR ) via the epidermal growth factor receptor ( EGFR ) . ^^^ METHODS : Expression of CAR and EGFR , and p 53 pathway and DNA damage responses were analyzed in six NB cell lines and two xenografts derived from primary NB using immunohistochemistry , reporter gene transactivation , Western blot and fluorescence activated cell sorting ( FACS ) analysis . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| In this report , we examined the role of cross talk between the CaR and the epidermal growth factor receptor ( EGFR ) in mediating these responses in Rat 1 cells . ^^^ These results indicate that EGFR transactivation is an important component of the CaR mediated response to increased Cao2+ in Rat 1 fibroblasts and most likely involves CaR mediated induction of regulated proteolysis and ligand shedding . . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| The CaR induced activation of ERK1 / 2 , induction of PTHrP release and stimulation of cellular proliferation in H 500 cells are likewise mediated , in large part , through the EGFR . ^^^ In conclusion , the calcium activates the EGFR , possibly through the CaR , to regulate downstream signaling events and important biological functions in a model of HHM . . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| Transduction of PEG containing , EGFR targeted viral complexes was inhibited by 66 % in coxsackie and adenovirus receptor ( CAR ) deficient RD cells and by 47 % in CAR expressing DU 145 cells in receptor antibody experiments . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| CaR effects on morphology were unaffected by inhibition of G ( q / 11 ) or G ( i / o ) signaling , epidermal growth factor receptor , or the metalloproteinases . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| Our results indicate that AMSH , which has been recently characterized as a regulator of the endosomal sorting of epidermal growth factor receptor , represents a novel modulator of CaR signaling . . ^^^ |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10644 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|