| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| The specific activity of four candidate protein tyrosine phosphatases ( protein tyrosine phosphatase 1B ( PTP1B ) , SH 2 domain containing PTPase 2 ( SHP 2 ) , leukocyte common antigen related ( LAR ) , and leukocyte antigen related phosphatase ) ( LRP ) toward IRS 1 dephosphorylation was studied using recombinant proteins in vitro . ^^^ |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| Here , insulin dependent insulin receptor tyrosine kinase activation was examined with recombinant insulin receptor substrate 1 ( IRS 1 ) as the substrate and shown to be 3 fold greater in cells with suppressed LAR levels . ^^^ Consistent with a receptor level effect , in vivo insulin dependent tyrosine phosphorylation of both IRS 1 and Shc was increased by a similar 3 fold with LAR suppression . ^^^ |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| After stimulation with 100 nM insulin , tyrosine phosphorylation of the insulin receptor was decreased by 31 % at 1 min ( P < 0 . 01 ) and by 42 % at 10 min ( P < 0 . 01 ) , and that of IRS 1 was decreased by 34 % ( P < 0 . 01 ) at 1 min and by 56 % ( P < 0 . 01 ) at 10 min in the LAR overexpressing cells compared with empty vector transfectants . ^^^ In contrast , overexpression of recombinant LAR cytoplasmic domain , detected as a 72 kDa protein in the cell cytosol , did not significantly affect the insulin stimulated tyrosine phosphorylation of the insulin receptor or IRS 1 ( 99 % and 93 % of control at 10 min , respectively ) . ^^^ |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| In order to determine whether the effects of TNF alpha might involve alterations in the expression of specific protein tyrosine phosphatases ( PTPases ) that have been implicated in the regulation of growth factor receptor signalling , KRC 7 rat hepatoma cells were treated with TNF alpha , and changes in overall tissue PTPase activity and the abundance of three major hepatic PTPases ( LAR , PTP1B , and SH PTP 2 ) were measured in addition to effects of TNF alpha on ligand stimulated autophosphorylation of insulin and epidermal growth factor ( EGF ) receptors and insulin stimulated insulin receptor substrate 1 ( IRS 1 ) phosphorylation . ^^^ |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| In addition , insulin stimulated tyrosine phosphorylation of the endogenous insulin receptor substrates IRS 1 and Shc were decreased to 57 % and 73 % of control , respectively , and IRS 1 associated phosphatidylinositol 3 ' kinase activity was reduced to 47 % of control of the cells overexpressing LAR . ^^^ |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| Finally , no covalent association between IR beta subunit and IRS 1 , the protein tyrosine phosphatase LAR or SHP 2 was observed in BMH treated cells expressing the wild type human IR . ^^^ |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10586 and P35568 |
Pubmed |
SVM Score :0.0 |
| NA |
|