| Interacting proteins: P50613 and P10276 |
Pubmed |
SVM Score :0.0 |
| Furthermore RAR alpha bound free CAK and purified TFIIH in vitro , and RAR alpha TFIIH complexes could be isolated from HeLa nuclear extracts . ^^^ |
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| Interacting proteins: P50613 and P10276 |
Pubmed |
SVM Score :0.0 |
| Here we find that the retinoid induced G ( 1 ) arrest and differentiation activation of cultured human leukemic cells are associated with a switch to CAK hypophosphorylation of retinoic acid receptor alpha ( RARalpha ) from CAK hyperphosphorylation of RARalpha . ^^^ The switch to CAK hypophosphorylation of RARalpha is accompanied by decreased MAT 1 expression and MAT 1 fragmentation that occurs in the differentiating cells through the all trans retinoic acid ( ATRA ) mediated proteasome degradation pathway . ^^^ Because HL60R cells that harbor a truncated ligand dependent AF 2 domain of RARalpha do not demonstrate any changes in MAT 1 levels or CAK phosphorylation of RARalpha following ATRA stimuli , these biochemical changes appear to be mediated directly through RARalpha . ^^^ These studies indicate that significant changes in MAT 1 levels and CAK activities on RARalpha phosphorylation accompany the ATRA induced G ( 1 ) arrest and differentiation activation , which provide new insights to explore the inversely coordinated P / D transition at G ( 1 ) exit . . ^^^ |
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| Interacting proteins: P50613 and P10276 |
Pubmed |
SVM Score :0.0 |
| Recent studies demonstrate that retinoic acid ( RA ) induced human HL 60 cell proliferation / differentiation ( P / D ) transition is accompanied by MAT 1 degradation and decreased CAK phosphorylation of retinoic acid receptor alpha ( RARa ) . ^^^ Thus , we investigated the biochemical pathway of MAT 1 degradation and its relationship with CAK phosphorylation of RARa . ^^^ RA induced MAT 1 ubiquitination reduces CAK abundance and decreases CAK phosphorylation of RARalpha , whereas inhibition of MAT 1 ubiquitination resists this RA effect . ^^^ These findings reveal that RA induces MAT 1 ubiquitination to decrease CAK phosphorylation of RARalpha , suggesting a novel mechanism of RA mediated P / D transition in which MAT 1 ubiquitination may act as an integral part of RA effect to decrease CAK activity in the switch from proliferation to differentiation . . ^^^ |
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| Interacting proteins: P50613 and P10276 |
Pubmed |
SVM Score :0.0 |
| The N terminal AF 1 domain of RARalpha is phosphorylated at S 77 by the cyclin dependent kinase ( cdk ) activating kinase ( CAK ) subcomplex ( cdk7 / cyclin H / MAT1 ) of the general transcription factor TFIIH . ^^^ |
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| Interacting proteins: P50613 and P10276 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that the XPD mutation alters cdk 7 function in RARalpha phosphorylation . ^^^ |
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| Interacting proteins: P50613 and P10276 |
Pubmed |
SVM Score :0.0 |
| NA |
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