| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Multiple cis acting sequences to which nuclear proteins bind have been mapped , and transcription factors that can bind to these sequences have been identified ; these include C / EBP alpha , Sp 1 , USF , HNF 1 , CTF / NF1 , the glucocorticoid receptor , and RAR alpha . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| In transient transfection studies in MCF 7 human breast cancer cells , pRAR 2 and pRAR 1 were E 2 responsive ; both of the RAR alpha 1 gene promoter inserts contained two GC rich sites and bound Sp 1 protein in gel mobility shift assays . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| As expected from previous studies , the transcription factor Sp 1 can be detected at the RARA alpha 1 promoter by ChIP . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Purified chick T3R alpha 1 ( cT3R alpha 1 ) binds as monomers and homodimers to a region in the LTR ( nucleotides 104 to 75 [ 104 / 75 ] ) which contains two tandem NF kappa B binding sites and to a region ( 80 / 45 ) which contains three Sp 1 binding sites . ^^^ Although the binding sites for p50 / 65 , p50 / 50 , and T3R , or Sp 1 and T3R , overlap , their binding is mutually exclusive , and with the inclusion of RXR beta , the major complex is the RXR beta cT3R alpha 1 heterodimer . ^^^ Mutations within the NF kappa B motifs which eliminate binding of cT3R alpha 1 also abolish stimulation by T 3 , indicating that cT3R alpha 1 binding to the Sp 1 region does not independently mediate activation by T 3 . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| ER alpha also interacts with Sp 1 protein to transactivate genes through binding Sp 1 ( N ) xERE or Sp 1 ( N ) xERE half site ( 1 / 2 ) motifs where both ER alpha and Sp 1 bind DNA elements . ^^^ Activation through Sp 1 ( N ) xERE1 / 2 requires interactions of both proteins with their cognate DNA elements as well as additional nuclear factors to form a functional ER alpha / Sp1 DNA complex . ^^^ Recent studies also show that ER alpha and Sp 1 physically interact and ER alpha preferentially binds to the C terminal DNA binding domain of Sp 1 protein . ^^^ Moreover , ER alpha / Sp1 can activate transcription from a consensus GC rich Sp 1 binding site in transient transfection studies in MCF 7 human breast cancer cells , and this response is also observed with ER alpha variants that do not contain the DNA binding domain . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| From chromatin immunoprecipitation ( ChIP ) assays , the nuclear retinoic acid receptor alpha ( RARalpha ) associated with the Sp 1 region , and RARs beta and gamma associated with the AP 1 and Sp 1 regions ; treatment of KG 1 cells with ATRA did not alter Sp 1 binding but increased the association of RARalpha and decreased the association of RARs beta and gamma . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| RAR gamma 2 expression is regulated through a retinoic acid response element embedded in Sp 1 sites . ^^^ Our data suggest an important role for RAR containing heterodimers and Sp 1 in the regulation of RAR gamma 2 expression . . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| The genomic sequences upstream of the start site cluster lack CAAT and TATA boxes , but have two Sp 1 binding sites and 10 T ( G / C ) AC ( C / A ) boxes , which may represent binding sites for RA receptors ( RAR ) . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| In the upstream important sequences , there were the nearest sequences for AP 1 binding motif , AP 2 binding element , SP 1 binding sequence , cAMP response element , half site of glucocorticoid receptor ( GRE ) binding sequence , half site of thyroid hormor receptor ( TR ) or retinoic acid receptor ( RAR ) binding sequence and MTF 1 binding sequence . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Computer analysis of the promoter proximal 1280 bp sequence revealed a number of potentially important cis regulatory sequences ; these include the recognition elements for Ap 1 , Ap 2 , Ap 3 , Sp 1 , a possible binding site for RAR : RXR , and a number of TCF 1 consensus motifs . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation studies , glutathione S transferase pull down experiment , and mammalian two hybrid assays suggested a physical interaction between RAR / RXR and Sp 1 . ^^^ Finally , Sp 1 and RAR / RXR synergistically enhanced the transactivation activity of the uPA promoter . ^^^ These results suggest that ( 1 ) RA induces RARs mainly via RARalpha and that ( 2 ) RAR / RXR physically and functionally interact with Sp 1 , resulting in a potentiation of uPA transcription . . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| The binding of Sp 1 to Sp 1 sites was independent of RAR RXR heterodimer but increased with the increase in Sp 1 concentration in the presence of unknown factor ( s ) of reticulocyte lysate . ^^^ We concluded that two genomic DNA regions , the DR 4 site ( 1531 to 1516 ) and the first and second Sp 1 binding sites ( 145 to 121 ) , were involved in the RA dependent augmentation of thrombomodulin gene expression through increased interactions of the two regions with heterodimer of RAR RXRalpha and nuclear Sp 1 , respectively . . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| In search for these missing links we found that nuclear receptors [ retinoic acid receptor ( RAR ) , thyroid hormone receptor ( TR ) , vitamin D ( 3 ) receptor , peroxisome proliferator activated receptor and retinoic 10 receptor ] induce an electrophoretic mobility increase of Sp 1 GC rich DNA complexes . ^^^ The presence of RAR and Sp 1 in complexes with either cognate site was revealed in supershift experiments . ^^^ In spite of similar capacity to form ternary complexes , RAR but not TR up regulated an Sp 1 driven reporter in a ligand dependent way . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Early granulopoiesis requires the C / EBPalpha , PU . 1 , RAR , CBF , and c Myb transcription factors , and terminal neutrophil differentiation is dependent upon C / EBPepsilon , PU . 1 , Sp 1 , CDP , and HoxA 10 . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Electrophoretic mobility shift assays showed that Sp 1 , but not RAR and / or RXR , could bind to the element . ^^^ The formation of the Sp 1 DNA complex was inhibited by co incubation with RAR , but not by RXR . ^^^ Taken together , these findings suggest that TXR gene transcription suppression may be mediated by the inhibition of Sp 1 binding to the 22 / 7 GC box related sequence by activated RAR , which may result in the inhibition of TX induced VSMC proliferation . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| HPR induced growth suppression was accompanied by a simultaneous block in the G ( 1 ) phase of the cell cycle in RAR transduced and control SP 1 cells and differential regulation of cell cycle and apoptotic mediators . . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| The transcription factors retinoic acid receptor ( RAR ) , retinoid 10 receptor ( RXR ) , and Sp 1 exhibited > 2 fold increased activity by HA ( 6 ) treatment , as confirmed by EMSA . ^^^ RT PCR analysis showed that the expression levels of MMP 3 , type 2 collagen , and COMP messenger RNA , which are tightly associated with the activation of RAR , RXR , or Sp 1 , were up regulated by treatment with HA ( 6 ) . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Chromatin immunoprecipitation assays indicate that HDAC inhibitors act on the FR beta gene by enhancing RAR associated histone acetylation to increase the association of Sp 1 with the basal FR beta promoter . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| In conclusion , our data indicate that light exposure increases VEGF expression through the mechanisms involving activation of Sp 1 and RARalpha signaling in Y 79 cells . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| Mutation of either the Sp 1 or the CBF site significantly decreases basal expression , transactivation by RXRalpha / RARalpha / RA , and the ability of TSA to stimulate hPDK 4 gene transcription . ^^^ |
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| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10276 and P08047 |
Pubmed |
SVM Score :0.0 |
| NA |
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