| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :1.1892739 |
| Potential interaction between CCR 1 and its ligand , CCL 3 , induced by endogenously produced interleukin 1 in human hepatomas . 1.1892739^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.58110956 |
| CCL 3 binds to the chemokine receptors CCR 1 and CCR 5 , which are expressed by inflammatory leukocytes such as macrophages and T cells present in the affected joints of RA patients . 0.58110956^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.56621805 |
| Inhibition of labeled MIP 1alpha binding was achieved with unlabeled MIP 1alpha and RANTES , the latter a beta chemokine that binds to both CCR 1 and CCR 5 , but not by MCP 1 . 0.56621805^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.5606907 |
| We previously reported that F4 / 80 ( ) B 220 ( ) CD11c ( + ) DC precursors expressing the CC chemokine receptors CCR 1 and CCR 5 are mobilized rapidly into the circulation in mice injected with Propionibacterium acnes and are recruited into inflammatory tissue by macrophage inflammatory protein 1alpha ( MIP 1alpha ) , which binds to CCR 1 and CCR 5 . 0.5606907^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Survival of CCR 5 ( / ) mice was equivalent to that of controls , indicating that the enhanced susceptibility of CCL 3 ( / ) mice to K . pneumoniae is mediated via another CCL 3 receptor , presumably CCR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In vivo neutralization of the CCR 1 ligand , macrophage inflammatory protein 1alpha ( CCL 3 ) , resulted in less encephalitogenic CD 4 ( + ) T cell CNS infiltration . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| An abortive ligand induced activation of CCR 1 mediated downstream signaling event and a deficiency of CCR 5 expression are associated with the hyporesponsiveness of human naive CD4+ T cells to CCL 3 and CCL 5 . ^^^ Thus , a failure of the ligand induced activation of CCR 1 mediated downstream signaling event as well as a deficiency of CCR 5 expression may be involved in the hyporesponsiveness of naive CD 4 ( + ) T cells to CCL 3 and CCL5 . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Moreover , CCL 3 L1 , but not CCL 3 , is susceptible to cleavage by CD 26 , creating a truncated 2 form with enhanced affinity for CCR 1 and CCR 5 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In parallel , CCR 1 and CCR 5 expression is down regulated and CXCL 8 , CCL 2 , CCL 3 and CCL 5 production is up regulated . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In contrast , CCL 3 , a ligand of CCR 1 and CCR 5 , induced rapid uptake of FITC dextran by immature DCs but not mature DCs . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| One such receptor is CCR 1 which is a receptor for CC chemokines , such as CCL 5 ( RANTES ) and CCL 3 ( MIP 1alpha ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Eosinophils from these donors showed increased eosinophil CCR 1 expression and also underwent CCL 3 mediated chemotaxis and up regulation of CD11b . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| After 2 to 4 weeks , L . amazonensis infected mice had significantly delayed and depressed expression of inflammatory cytokines ( interleukin 12 [ IL 12 ] , gamma interferon , IL 1 alpha , IL 1 beta ) , CC chemokines ( CC chemokine ligand 3 [ CCL 3 ] / macrophage inflammatory protein 1 alpha [ MIP 1 alpha ] , CCL4 / MIP 1 beta , CCL5 / RANTES , MIP 2 ) , and chemokine receptors ( CCR 1 , CCR 2 , CCR 5 ) in foot tissues and draining lymph nodes compared to the expression in L . major infected controls . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The chemokines CCL 3 and CCL 5 , as well as their shared receptor CCR 1 , are believed to play a role in the pathogenesis of several inflammatory diseases including rheumatoid arthritis , multiple sclerosis , and transplant rejection . ^^^ Radiolabeled binding studies indicate that CP 481 , 715 binds to human CCR 1 with a Kd of 9 . 2 nm and displaces 125I labeled CCL 3 from CCR 1 transfected cells with an IC 50 of 74 nm . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization histochemistry revealed local production of the CCR 1 ligands CCL 3 ( MIP 1 alpha ) and CCL 5 ( RANTES ) , as well as large numbers of CCR 1 and CCR 5 expressing cells within inflammatory brain lesions . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In addition , chemotaxis toward the CCR 1 and CCR 5 ligands CCL 3 and CCL 5 was also increased . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The present manuscript details the discovery and early fundamental structure activity relationship studies involving compound 3 , a novel hydroxyethylene peptide isostere derived molecule that provides micromolar inhibition of CCL 3 binding to its receptor CCR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| This was associated with reduced renal expression of CC chemokines CCL 2 , CCL 3 , CCL 4 , and CCL 5 and the chemokine receptors CCR 1 , CCR 2 , and CCR 5 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In the present study , pretreatment of CCR 1 : mu opioid receptor / HEK293 cells with CCL 3 ( MIP 1alpha ) induced internalization of mu opioid receptors and severely impaired the mu opioid receptor mediated inhibition of cAMP accumulation . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Costimulation via FcepsilonRI engagement with IgE / antigen and CCR 1 engagement with recombinant human CCL 3 synergistically enhanced degranulation in rat basophilic leukemia 2H3 cells expressing human CCR 1 ( RBL CCR 1 ) . ^^^ Interestingly , FcepsilonRI engagement inhibited CCL 3 mediated chemotaxis and membrane ruffling of RBL CCR 1 cells . ^^^ Both a Rho inhibitor C 3 exoenzyme and a Rho kinase ( ROCK ) inhibitor Y 27632 inhibited chemotaxis of RBL CCR 1 cells toward CCL 3 , indicating that activation of the Rho / ROCK signaling pathway is required for the CCL 3 mediated chemotaxis of the cells . ^^^ Costimulation with IgE / antigen and CCL 3 enhanced Rac and Cdc 42 activation but decreased ROCK activation in RBL CCR 1 cells compared with that in the cells stimulated with CCL 3 alone . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| To assess the role of the chemokine receptors CCR 1 and CCR 5 , and a ligand for these receptors CCL3 / macrophage inflammatory protein 1alpha , in this pathogenic process , the reverse passive cutaneous Arthus reaction was induced in mice lacking CCR 1 , CCR 5 , or CCL 3 . ^^^ Edema was significantly attenuated in CCR 1 deficient ( CCR 1 ( / ) ) and CCL 3 ( / ) mice but not CCR 5 ( / ) mice , compared with wild type mice . ^^^ Numbers of infiltrating neutrophils and mast cells were reduced in CCL 3 ( / ) and CCR 1 ( / ) mice , respectively , compared with wild type mice . ^^^ Remarkably , the intradermal mRNA expression of CCL5 / RANTES , another ligand for CCR 1 and CCR 5 , was increased in CCR 5 ( / ) and CCL 3 ( / ) mice , compared with wild type mice , while the cutaneous CCL 3 mRNA expression was augmented in CCR 1 ( / ) and CCR 5 ( / ) mice . ^^^ These results indicate that CCR 1 , CCR 5 , and CCL 3 cooperatively contribute to the cutaneous Arthus reaction , and also suggest that enhanced expression of CCL 3 and CCL 5 compensates for the loss of CCR 1 , CCR 5 , and CCL 3 in the reaction . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Thirty three point mutants of CCR 1 were expressed transiently in L1 . 2 cells , and the cells were assessed for their capacity to migrate in response to CCL 3 in the presence or absence of UCB 35625 . ^^^ Cells expressing the mutant constructs Y41A ( TM helix 1 , or TM 1 ) , Y113A ( TM 3 ) , and E287A ( TM 7 ) were responsive to CCL 3 but resistant to the antagonist , consistent with a role for the TM helices in CCR 1 interactions with UCB 35625 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The mRNA expression of CCR 1 and CC chemokines , including the CCR 1 ligands CCL 3 ( MIP 1alpha ) and CCL 5 ( RANTES ) , was up regulated in diseased kidneys , with a prominent interstitial expression of CCL 5 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The expression of CCL 3 ( MIP 1alpha ) , CCL 4 ( MIP 1beta ) , CCL 5 ( RANTES ) and their main receptors ( CCR 1 and CCR 5 ) was studied by semi quantitative reverse transcriptase polymerase chain reaction ( RT PCR ) and immunohistochemistry in a series of 16 IIM and five controls ( four normal muscles and one tonsil ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| When CCR 1 : TRPV1 / HEK293 cells were pretreated with CCL 3 , the sensitivity of TRPV 1 , as indicated by the Ca ( 2+ ) influx , was increased approximately 3 fold . ^^^ CCR 1 on DRG neurons was functional , as demonstrated by CCL 3 induced Ca ( 2+ ) ion influx and PKC activation . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| A minority of BM MSCs ( 2 % to 25 % ) expressed a restricted set of chemokine receptors ( CXC receptor 4 [ CXCR 4 ] , CX3C receptor 1 [ CX3CR1 ] , CXCR 6 , CC chemokine receptor 1 [ CCR 1 ] , CCR 7 ) and , accordingly , showed appreciable chemotactic migration in response to the chemokines CXC ligand 12 ( CXCL 12 ) , CX3CL1 , CXCL 16 , CC chemokine ligand 3 ( CCL 3 ) , and CCL 19 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| As for chemokines and chemokine receptors , CXCL1 / GRO alpha , CXCL2 / GRO beta , CXCL5 / ENA 78 , CXCL6 / GCP2 , IL 8 / CXCL8 , CXCL12 / SDF 1 , CXCL9 / Mig , CXC11 / ITAC , CX3CL1 / fractalkine , CCL2 / MCP 1 , CCL 3 , CCL5 / RANTES , CCL 7 , CCL 15 , CCL 21 , CCL 23 , CCL 28 , and CCR 1 , CCR 9 , CXCR 4 were identified . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| MIP 1alpha [ CCL 3 ] acting on the CCR 1 receptor mediates neutrophil migration in immune inflammation via sequential release of TNF alpha and LTB 4 . ^^^ Herein , we show increased mRNA expression for MIP 1alpha [ CCL 3 ] , MIP 1beta [ CCL 4 ] , RANTES [ CCL 5 ] , and CCR 1 in peritoneal cells harvested from OVA challenged , immunized mice , as well as MIP 1alpha [ CCL 3 ] and RANTES [ CCL 5 ] but not MIP 1beta [ CCL 4 ] proteins in the peritoneal exudates . ^^^ MIP 1alpha [ CCL 3 ] used CCR 1 to promote neutrophil recruitment , as OVA or MIP 1alpha [ CCL 3 ] failed to induce neutrophil migration in CCR 1 ( / ) mice , in contrast to CCR 5 ( / ) mice . ^^^ In summary , we have demonstrated that neutrophil migration observed in this model of immune inflammation is mediated by MIP 1alpha [ CCL 3 ] , which via CCR 1 , induces the sequential release of TNF alpha and LTB ( 4 ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| From each blood sample PBMCs were separated and gene expression levels for chemokines CCL 3 , CCL 4 , CCL 5 , CXCL 10 , and their receptors CCR 1 , CCR 5 , and CXCR 3 , were determined using real time quantitative PCR . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Met RANTES , an amino terminal modified methionylated form of RANTES ( CCL 5 ) , antagonizes the binding of the chemokines RANTES and macrophage inflammatory protein 1alpha ( MIP 1alpha ; CCL 3 ) to their receptors CCR 1 and CCR 5 , respectively . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Differential pattern of CCR 1 internalization in human eosinophils : prolonged internalization by CCL 5 in contrast to CCL 3 . ^^^ A significant down regulation of CCR 1 was induced by 24 h preincubation of isolated eosinophils from CCR1high donors either with IL 3 , CC chemokine ligand 3 ( CCL 3 ) , CCL 5 , CCL 7 , or CCL 13 . ^^^ Internalization experiments using eosinophils from CCR1high donors revealed that CCL 5 is more effective to induce CCR 1 internalization than CCL 3 . ^^^ Whereas CCR 1 re expression after stimulation with CCL 3 reached prestimulation levels ( 120 min : 81 . 3 % relative CCR 1 surface expression ) CCL 5 induced a prolonged CCR 1 internalization ( 120 min : 15 . 7 % ) . ^^^ CONCLUSIONS : This study demonstrates a distinct pattern of CCR 1 internalization and re expression in human eosinophils between CCL 3 and CCL 5 , as CCL 5 induces a prolonged CCR 1 internalization and the basic value is not reached after 24 h . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Essential contribution of a chemokine , CCL 3 , and its receptor , CCR 1 , to hepatocellular carcinoma progression . ^^^ Here , we show that CCL 3 and CCR 1 are also expressed in 2 different models of this cancer ; N nitrosodiethylamine ( DEN ) induced HCC and HCC induced by hepatitis B virus surface ( HBs ) antigen primed splenocyte transfer to myelo ablated syngeneic HBs antigen transgenic mice . ^^^ At 10 months after DEN treatment , foci number and sizes were remarkably reduced in CCR 1 and CCL 3 deficient mice , compared with those of wild type ( WT ) mice , although tumor incidence were marginally , but significantly , higher in CCR 1 and CCL 3 deficient mice than in WT mice . ^^^ Of note is that tumor angiogenesis was also markedly diminished in CCL 3 and CCR 1 deficient mice , with a concomitant reduction in the number of intratumoral Kupffer cells , a rich source of growth factors and matrix metalloproteinases ( MMPs ) . ^^^ Moreover , MMP 9 , but not MMP 13 , gene expression was attenuated in CCR 1 and CCL 3 deficient mice , compared with that of WT mice . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| U83A Npep acted only as antagonist , efficiently blocking binding of CCL 3 to CCR 1 or CCR 5 on differentiated monocytic / macrophage leukemic cells . ^^^ Furthermore , CCL 3 induction of calcium signaling via CCR 1 and CCL 1 induced chemotaxis via CCR 8 in primary human leukocytes was inhibited . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| We have found that costimulation with IgE / antigen and CC chemokine ligand 3 ( CCL 3 ) enhances degranulation but inhibits chemotaxis of rat basophilic leukaemia ( RBL ) 2H3 cells expressing human CCR 1 ( RBL CCR 1 cells ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| We therefore studied the displacement of [ 125I ] MIP 1 alpha by IL 8 Leu 25 > Tyr from the CC CKR 1 receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Characterisation of the RANTES / MIP 1 alpha receptor ( CC CKR 1 ) stably transfected in HEK 293 cells and the recombinant ligands . ^^^ In order to study the activation of a single receptor , we have expressed the shared receptor ( CC CKR 1 ) for RANTES and MIP 1 alpha stably in the HEK 293 cell line . ^^^ We have examined the effects of RANTES and MIP 1 alpha on the CC CKR 1 transfectants by equilibrium binding studies and in a chemotaxis assay . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| We have previously cloned three mouse genes by cross hybridization with the human MIP 1 alpha / RANTES receptor gene CMKBR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| However , we now show that MCP 3 is a ligand and potent agonist for the macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) / regulated on activation , normal T expressed , and secreted protein ( RANTES ) receptor CC CKR 1 ( rank order for [ Ca2+ ] 1 changes = MIP 1 alpha > MCP 3 > RANTES ) , which is expressed in monocytes > neutrophils > eosinophils . 125I MCP 3 bound directly to CC CKR 1 and CC CKR2B ( Ki = 8 and 7 nM , respectively ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| CC CKR 3 is a seven transmembrane domain G protein coupled receptor most closely related to the previously reported monocyte and neutrophil selective receptor CC CKR 1 ( also known as the MIP 1 alpha / RANTES receptor ) . ^^^ When [ Ca2+ ] 1 changes were monitored in stably transfected human embryonic kidney 293 cells , MIP 1 alpha and RANTES were both potent agonists for CC CKR 3 and CC CKR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Eosinophils also express a much lower level of a second chemokine receptor , CC CKR 1 , which appears to be responsible for the effects of MIP 1 alpha . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In this report we show that the deletion of another receptor for MIP1alpha and RANTES , the CCR 1 receptor , is associated with protection from pulmonary inflammation secondary to acute pancreatitis in the mouse . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| A chemokine receptor cDNA clone , designated YT 4 , was sequenced and found to be identical to the known C C CKR 1 or macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) / Rantes receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Two human beta chemokine receptors have been cloned and characterized : the MIP 1 alpha / RANTES receptor or C C chemokine receptor 1 ( CCR 1 ) and the MCP 1 receptor or C C chemokine receptor 2 ( CCR 2 ) . ^^^ COS cells transiently transfected with the murine homologue of human CCR 1 bind murine MIP 1 alpha and human RANTES with Kds of 3 . 4 nM and 4 . 2 nM and murine MIP 1 beta with an EC 50 of 8 . 9 nM . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Human and murine macrophage inflammatory protein 1 alpha ( MIP 1 alpha ) , human human monocyte chemotactic protein 1 ( MCP 1 ) , and RANTES all bind to the C C CKR 1 with varying affinities . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The same pattern was observed in peritoneal neutrophils from wild type mice , whereas neutrophils from CCR 1 / mice failed to respond to either MIP 1 alpha or Lkn 1 . ^^^ We conclude that CCR 1 is a receptor mediating responses to both MIP 1 alpha and Lkn 1 on neutrophils and produces different biological responses depending on the ligand bound . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Identification of amino acids involved in the binding of hMIP 1 alpha to CC CKR 1 , a MIP 1 alpha receptor found on neutrophils . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| This CCR 1 was thought to be functional as assessed by neutrophil CD11b up regulation following whole blood MIP 1 alpha stimulation . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Here we examine the role of MIP 1 alpha in the pathogenesis of this disease using mice deficient in MIP 1 alpha or its receptor , CCR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| For examples , Langerhans cells migrate selectively to MIP 3 alpha ( via CCR 6 ) , blood CD11c+ DC to MCP chemokines ( via CCR 2 ) , monocytes derived DC respond to MIP 1 alpha / beta ( via CCR 1 and CCR 5 ) , while blood CD11c DC precursors do not respond to any of these chemokines . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In EAE protected mice , 17 beta estradiol strongly inhibited mRNA expression of the chemokines RANTES , MIP 1 alpha , MIP 2 , IP 10 , and MCP 1 , and of the chemokine receptors CCR 1 , CCR 2 and CCR 5 at both time points . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Although only a minority of the cell lines responded by calcium mobilization after agonist stimulation , a migratory response to the CCR 1 ligands RANTES and MIP 1 alpha was obtained in 5 / 6 and 4 / 6 , respectively , of the cell lines tested . ^^^ The expression of CXCR 4 and CCR 1 and the migration to their ligands , SDF 1 , and RANTES and MIP 1 alpha , respectively , were also demonstrated in primary MM cells . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Cloning and differential tissue specific expression of three mouse beta chemokine receptor like genes , including the gene for a functional macrophage inflammatory protein 1 alpha receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The predicted protein sequence of K 5 5 shows only 49 % identity to the macrophage inflammatory protein 1 alpha / RANTES receptor ( CC CKR 1 ) and 47 % identity to monocyte chemotactic protein 1 receptor ( b form ) , suggesting that this cDNA encodes a novel member of the CC chemokine receptor family . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| This Leu 25 > Cys NBD mutant introduces monocyte chemoattractant activity and the ability to displace 125I labeled macrophage inflammatory protein 1 alpha from the recombinant CC CKR 1 receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Cloning and characterization of a novel murine macrophage inflammatory protein 1 alpha receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| After bleomycin challenge in C57BL / 6J mice , the expression of CCR 1 mRNA increased and peaked at day 7 , which paralleled to the expression of its ligands , macrophage inflammatory protein 1 alpha and RANTES . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Our studies showed that LD infection caused the upregulation of three beta chemokines ( macrophage inflammatory protein 1 alpha ( MIP 1alpha ) , MIP 1beta and RANTES ( regulated on activation normal T cell expressed and secreted ) ) , one alpha chemokine ( interleukin 8 ( IL 8 ) ) and the CC chemokine receptor 5 ( CCR 5 ) but not CCR 1 , as evident from reverse transcriptase polymerase chain reaction ( RT PCR ) analysis . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Radiolabeled MCP 1 bound with high affinity to the wild type MCP 1 receptor , but not to the RANTES / MIP 1alpha receptor ( C C CKR 1 ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Preparation of specific polyclonal antibodies to a C C chemokine receptor , CCR 1 , and determination of CCR 1 expression on various types of leukocytes . cDNA cloning has revealed the presence of at least three distinct human receptors for macrophage inflammatory protein 1alpha ( MIP 1alpha ) and RANTES : C C chemokine receptor ( CCR ) 1 , 4 , and 5 . ^^^ Finally , the antibody partially inhibited monocyte chemotactic activities of human rMIP 1alpha , suggesting that CCR 1 is a functional receptor for MIP 1alpha on human peripheral blood monocytes . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Receptor studies have indicated that the inactivity of Hep Mut on human monocytes correlates with its inability to bind to CCR 1 , a cloned human MIP 1alpha receptor . ^^^ In addition , studies using proteoglycan deficient cells transfected with CCR 1 have indicated that the proteoglycan binding site in MIP 1alpha is a site that is also involved in the docking of MIP 1alpha to the monocyte receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| R46A bound as efficiently as wild type MIP 1alpha to CCR 1 and was equally active in eliciting increases in intracellular free calcium concentrations . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In experiments using cells transfected with C C chemokine receptors , 125I MCP 2 bound to human embryonic kidney 293 cells transfected with CCR 1 or CCR2B , known to also bind MIP 1alpha / RANTES and MCP 1 , respectively , but both shared by MCP 3 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Chinese hamster ovary cells expressing CCR 1 , 3 , 5 , and D 6 bound 125I murine MIP 1alpha : the order of affinity was D 6 > CCR 5 > CCR 1 > CCR 3 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Binding assays using 125I labeled C C chemokines in mammalian cells indicated that CHEMR 1 did not bind MIP 1alpha , RANTES , or MIP 1beta , whereas CCR 1 binds MIP 1alpha and RANTES . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In order to observe modulations of MIP 1alpha secretion and expression along with MIP 1alpha receptor ( MIP 1alpha R ) expression for a possible autocrine role in AIDS associated B cell lines , we studied a wide panel of human B cell lines . ^^^ Furthermore , our results strongly suggest MIP 1alpha autocrine loops in a majority of tumor B cells as evident by MIP 1alpha R expression , and also secretion of MIP 1alpha . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| To clarify the mechanisms for the modulation of hematopoiesis by this chemokine , we examined the expression of a receptor for MIP 1alpha , CCR 1 , on bone marrow cells of normal individuals using a specific antibody and explored the effects of MIP 1alpha on in vitro erythropoiesis driven by stem cell factor ( SCF ) and erythropoietin ( Epo ) . ^^^ Although reverse transcription polymerase chain reaction ( RT PCR ) showed the presence of CCR 1 , CCR 4 , and CCR 5 transcripts in CD34+ cells in BM , anti CCR 1 antibodies significantly abrogated the inhibitory effects of MIP 1alpha on BFU E formation both in a methylcellulose culture and in a single cell proliferation assay of purified CD34+ cells . ^^^ Although the contribution of CCR 4 or CCR 5 can not be completely excluded , these results suggest that MIP 1alpha mediated suppression of the proliferation of immature , but not mature erythroid progenitor cells , is largely mediated by CCR 1 expressed on these progenitor cells . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| RT PCR and in situ hybridization were used to identify expression of the murine CCR 1 MIP 1alpha receptor on astrocytes . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Finally , AMD 3100 had no effect on the Ca2+ flux induced by the CC chemokines MIP 1alpha , regulated on activation normal T cell expressed and secreted ( RANTES ; also a ligand for CCR 5 ) , or monocyte chemoattractant protein 3 ( a ligand for CCR 1 and CCR2b ) , nor was it able to induce Ca2+ fluxes by itself . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Desensitization studies indicated that RANTES , MIP 1alpha and MCP 3 interacted at the same receptor , which is identical in characterization to the cloned CCR 1 . 125I MCP 1 also demonstrated high affinity satuable binding to dEoL 3 cells with a Kd value of 0 . 4 nM . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Removal of glycosaminoglycans from CHO cells expressing chemokine receptors CXCR 1 , CCR 1 , or CCR 2 resulted in 40 70 % decreases in the binding of RANTES , MCP 1 , IL 8 , and MIP 1alpha . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| RANTES and MIP 1alpha mediate their effects via shared chemokine receptors ( CCRs ) : CCR 1 , CCR 4 , and CCR 5 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Messenger RNA for the MIP 1alpha / RANTES receptor , CCR 1 , and the MCP 1 / MCP 3 receptor , CCR 2 , was expressed by unstimulated VSMCs grown under serum free culture conditions for 24 hours . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| This is the first description of CCR 1 receptor antagonists that may be useful in the treatment of chronic inflammatory diseases involving MIP 1alpha , RANTES , and CCR1 . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The N terminal extracellular segments of the chemokine receptors CCR 1 and CCR 3 are determinants for MIP 1alpha and eotaxin binding , respectively , but a second domain is essential for efficient receptor activation . ^^^ In particular , macrophage inflammatory protein 1alpha ( MIP 1alpha ) is a ligand for CCR 1 but not CCR 3 , and eotaxin is a ligand for CCR 3 but not CCR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| HCC 1 competed with MIP 1alpha for binding to CCR 1 transfected cells , but with a markedly reduced affinity ( IC 50 = 93 nM versus 1 . 3 nM for MIP 1alpha ) . ^^^ Similarly , HCC 1 was less potent than MIP 1alpha in inducing inhibition of adenylyl cyclase in CCR 1 transfected cells . ^^^ HCC 1 induced chemotaxis of freshly isolated human monocytes , THP 1 cells , and CCR 1 transfected cells , and the optimal concentration for cell migration ( 100 nM ) was approximately 100 fold lower than that of MIP 1alpha ( 1 nM ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| However , when Northern blot analysis was used , mRNA for two different MIP 1alpha receptors CCR 1 and CCR 5 could be detected in bone marrow , but only CCR 1 mRNA was seen in cord blood CD34+ samples . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The expression of the MIP 1alpha receptor subtypes on LP CD 34 ( + ) cells was studied by indirect immunofluorescence using specific antibodies for the detection of CCR 1 , CCR 4 , and CCR 5 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| MPIF 1 binds specifically to sites , in particular CCR 1 , shared with macrophage inflammatory protein 1alpha ( MIP 1alpha ) on the surface of human monocytes and dendritic cells , as inferred by its ability to compete for [ 125I ] MIP 1alpha , but not for [ 125I ] MIP 1beta or [ 125I ] monocyte chemotactic protein 1 ( MCP 1 ) binding to intact cells . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Here we report that superantigen stimulation of human peripheral blood monocytes results in a rapid , dose dependent , and specific down regulation of chemokine ( macrophage inflammatory protein 1alpha ( MIP 1alpha ) , monocyte chemotactic protein 1 and MIP 1beta ) binding sites ( e . g . , CCR 1 , CCR 2 , and CCR 5 ) , which correlates with a concomitant hyporesponsiveness of human monocytes to these CC chemokine ligands . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In contrast , MIP 1alpha induced a potent response in eosinophils from a small and previously undescribed subgroup of donors via a non CCR 3 pathway likely to be CCR 1 mediated . ^^^ MIP 1alpha responsiveness in vivo may therefore be a function of both CCR 1 expression levels and the regulated efficiency of coupling to intracellular signaling pathways . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| MIP 1alpha and MIP 5 were found to be the most effective chemoattractants , CCR 1 was the main receptor expressed and modulated during LPS treatment , and MIP 2 , RANTES , IP 10 and MCP 1 were the chemokines modulated during DC maturation . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In a parallel manner , binding of labeled MIP 1alpha was first shown to be attenuated by unlabeled RANTES , both of which recognize CCR 1 and CCR 5 , and then separately antagonized by MCP 3 and MIP 1beta , which bind to CCR 1 and CCR 5 , respectively . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Ligands for the CCR 1 receptor ( MIP 1alpha and RANTES ) have been implicated in a number of chronic inflammatory diseases , most notably multiple sclerosis and rheumatoid arthritis . ^^^ A novel series of 4 hydroxypiperidines has been discovered by high throughput screening ( HTS ) which potently inhibits the binding of MIP 1alpha and RANTES to the recombinant human CCR 1 chemokine receptor . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| During the development of nephrotoxic nephritis ( NTN ) in the mouse , we find that a variety of chemokines and chemokine receptors are induced : CCR 1 ( RANTES , MIP 1alpha ) , CCR 2 ( MCP 1 ) , CCR 5 ( RANTES , MIP 1alpha , MIP 1beta ) , CXCR 2 ( MIP 2 ) , and CXCR 3 ( IP 10 ) . ^^^ Lastly , using recombinant proteins and transfected cells that overexpressed CCR 1 , we demonstrated that MIP 1alpha , but not RANTES , bound CCR 1 and induced cell chemotaxis . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The increase in CCR 1 expression was reflected in an increase in the number of cell surface receptors for MIP 1alpha on the GM CSF treated BMM and in an enhanced response of the GM CSF treated BMM to CCR 1 ligands . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Here , the polypeptide specifically induced migration of U 937 cells expressing the CCR 1 ( MIP 1alpha receptor ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| We conclude that CCR 1 is a receptor for different length CKbeta 8 and that CKbeta 8 ( 25 99 ) has a similar affinity for CCR 1 as macrophage inflammatory protein 1alpha ( MIP 1alpha ) . ^^^ The longer variants of CKbeta 8 are significantly less potent than CKbeta 8 ( 25 99 ) and MIP 1a on CCR 1 and monocytes ( P < 0 . 05 ) . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| To determine if CCR 1 was dominantly mediating the above activities of MIP 1alpha , CCR 1 deficient ( / ) mice , produced by targeted gene disruption , were used . ^^^ MIP 1alpha enhanced colony formation of marrow granulocyte / macrophage colony forming units ( CFU GM ) , responsive to stimulation by granulocyte / macrophage colony stimulating factor ( GM CSF ) , and CFU M , responsive to stimulation by M CSF , from littermate control CCR 1 ( + / + ) but not CCR 1 ( / ) mice . ^^^ Moreover , MIP 1alpha did not mobilize MPCs to the blood or synergize with G CSF in this effect in CCR 1 ( / ) mice . ^^^ Multi growth factor stimulated MPCs in CCR 1 ( / ) and CCR 1 ( + / + ) marrow were equally sensitive to inhibition by MIP 1alpha . ^^^ These results implicate CCR 1 as a dominant receptor for MIP 1alpha enhancement of proliferation of lineage committed MPCs and for mobilization of MPCs to the blood . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Expression of MIP 1alpha R mRNA in these cells suggests an autocrine role for this chemokine in HTLV 1 infected T cells . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| TGFbeta 1 mediated increase of CCR 1 mRNA accumulation resulted in increased CCR 1 protein expression and augmented cell migration to a physiological ligand , macrophage inflammatory protein 1alpha ( MIP 1alpha ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| However , all three active chemokines have been reported to bind to CCR 1 and cross desensitization studies demonstrate that RANTES and MIP 1alpha can partially inhibit the chemotactic response elicited by CKbeta 8 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Competition binding studies revealed that BX 471 was able to displace the CCR 1 ligands macrophage inflammatory protein 1alpha ( MIP 1alpha ) , RANTES , and monocyte chemotactic protein 3 ( MCP 3 ) with high affinity ( K ( 1 ) ranged from 1 nm to 5 . 5 nm ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In this study , Chinese hamster ovary cells were transfected separately with the human chemokine receptors CCR 1 and CCR 5 , and these receptors were shown to induce an intracytoplasmic Ca ( 2+ ) flux and cellular chemotaxis following stimulation with the natural CC chemokine ligands ( MIP 1alpha , RANTES ( regulated on activation normal T cell expressed ) , and MIP 1beta ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Gene transcripts for the receptor CCR 1 , that binds RANTES and MIP 1alpha were also readily detected , as was CCR 2 , the receptor for the monocyte chemotactic proteins ( MCP ) 1 4 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Nanomolar concentrations of UCB 35625 were sufficient to inhibit eosinophil shape change responses to MIP 1alpha , MCP 4 , and eotaxin , while greater concentrations could inhibit the chemokine induced internalization of both CCR 1 and CCR 3 . ^^^ Chemotaxis of transfected cells expressing either CCR 1 or CCR 3 was inhibited by nanomolar concentrations of the compound ( IC ( 50 ) values of CCR 1 MIP 1alpha = 9 . 6 nm , CCR 3 eotaxin = 93 . 7 nm ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| However , it failed to significantly displace radiolabeled macrophage inflammatory protein 1alpha ( MIP 1alpha ) binding to mouse CCR 1 at concentrations up to 10 microM . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The number of CCR 1 positive cells in glomeruli was correlated with urinary levels of MIP 1alpha . ^^^ The number of CCR 1 positive cells in the interstitium was correlated with both urinary MIP 1alpha and RANTES levels . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| A macrophage inflammatory protein 1alpha ( MIP 1alpha ) receptor has been cloned from rat ( Rattus norvegicus ) genomic DNA by PCR using oligonucleotides based on the mouse CCR 1 DNA sequence and expressed in HEK 293 cells to enable its characterization . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To investigate the kinetics of expression of MIP 1alpha and receptor CCR 1 and the relationships between the expression and infiltration of inflammatory cells in allergen induced cutaneous late phase reactions in atopic subjects . ^^^ RESULTS : The peak expression of allergen induced mRNA for MIP 1alpha and CCR 1 was 6 h . ^^^ CONCLUSION : Allergen induced cutaneous late phase reactions in humans were associated with increased expression of MIP 1alpha and CCR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In this study , a group of healthy subjects over 90 years old is compared to a group of young subjects , we evaluated the ability of monocytes , T lymphocytes and NK cells : ( 1 ) to produce RANTES and MIP 1alpha , either in basal conditions or after stimulation with , respectively , LPS , anti CD 3 MoAb and IL 2 ; ( 2 ) to express the corresponding chemokine receptors ( CCR 1 , CCR 3 , CCR 5 ) . ^^^ We demonstrate that : ( a ) monocytes , T lymphocytes and NK cells spontaneously produced detectable amounts of chemokines , both in young and old subjects ; ( b ) monocyte dependent RANTES and MIP 1alpha production induced by LPS was up regulated in nonagenarian subjects as anti CD 3 induced secretion from T cells ; ( c ) RANTES and MIP 1alpha production by IL 2 stimulated NK cells was reduced in elderly subjects ; ( d ) CCR 1 , CCR 3 and CCR 5 were widely expressed on monocytes , but less expressed on T lymphocytes and NK cells . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemistry assays were also used to detect 2 MIP 1alpha receptors , CCR 1 and CCR 5 . ^^^ In P 7 to P 12 brain , many cells expressed MIP 1alpha receptors ; both CCR 1 and CCR 5 immunoreactivity were localized to endothelium and ependyma ; CCR 1 immunoreactive astrocytes and neurons and CCR 5 immunoreactive microglia were also identified . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In earlier work , we found that acute infection with the natural rodent pathogen , PVM , elicited a rapid and sustained pulmonary inflammatory response ( peak , 1 . 7 10 10 ( 6 ) leukocytes / ml BAL fluid ) that was dependent on both local production of MIP 1alpha and signaling via its receptor , CCR 1 . ^^^ Further experiments with CCR 1 deficient mice confirm the finding that although MIP 1alpha is produced in response to RSV infection , leukocytes do not respond via this pathway . ^^^ Among the explanations for these findings , we propose that there are other , as yet to be identified proinflammatory mediators elicited in response to PVM ( but not in response to RSV ) that serve to prime the leukocytes in vivo , thus enabling them to respond to MIP 1alpha signaling via CCR 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| We examined one MIP 1alpha receptor , CCR 1 , which is present on CD34+ cells from haemopoietic tissues . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The mRNAs for MIP 1alpha and MCP 1 , as well as their cognate receptors CCR 1 and CCR 2 , respectively , were localized in resident and infiltrating macrophages in numbers commensurate with those of F4 / 80 immunopositive macrophages in adjacent tissue sections . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| We also evaluated the expression of the CCR 1 ligand macrophage inflammatory protein 1alpha ( MIP 1alpha / CCL3 ) . ^^^ MIP 1alpha positive cells were frequently located near the processes and cell bodies of CCR 1 immunoreactive cells , during times of neuronal and glial maturation ( second and third postnatal weeks ) . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Toxin A induced in all mice a significant ( P < 0 . 05 ) increase in ileal fluid accumulation , epithelial damage , and neutrophil infiltration , with all parameters being significantly ( P < 0 . 01 ) lower in CCR 1 / and MIP 1alpha / mice . ^^^ Ileal messenger RNA expression of the CCR 1 ligands MIP 1alpha and RANTES and RANTES synthesis were increased in toxin A treated wild type mice . ^^^ CONCLUSIONS : C . difficile toxin A induced murine enteritis involves CCR 1 and its ligands MIP 1alpha and RANTES , which may be important mediators of the neutrophil recruitment characterizing acute , enterotoxin mediated enteritis . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Identification of the extracellular loop 2 as the point of interaction between the N terminus of the chemokine MIP 1alpha and its CCR 1 receptor . ^^^ Macrophage inflammatory peptide 1alpha ( MIP 1alpha ) / CC chemokine receptor ligand 3 is an 8 kDa peptide that induces chemotaxis of various lymphocytes to sites of inflammation through interaction with the G protein coupled chemokine receptors CCR 1 and CCR 5 . ^^^ Benzophenone MIP 1alpha is a full agonist that specifically and covalently labels CCR 1 and CCR 5 receptors upon irradiation . ^^^ In the present study , we use enzymatic and chemical cleavage methods on wild type and mutated CCR 1 receptors to show that the N terminus of the chemokine MIP 1alpha interacts in a specific manner with the second extracellular loop of the CCR 1 receptor , within a segment comprising amino acids 178 to 194 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Although originally referred to as the MIP 1alpha / RANTES receptor , CCR 1 is quite promiscuous and can be activated by numerous chemokines . ^^^ We used radioligand binding and [ 35S ] GTPgammaS exchange assays in membranes from a cell line transfected to express CCR 1 ( Ba / F3 hCCR 1 ) to characterize a panel of chemokines ( HCC 1 , MIP 1alpha , MIP 1beta , MIP 1delta , MPIF 1 , MCP 2 , MCP 3 , and RANTES ) as CCR 1 ligands . ^^^ Using [ 35S ] GTPgammaS exchange assays , intracellular calcium flux and / or whole cell chemotaxis assays in HL 60 ( Rx ) cells , we validated that MIP 1alpha was the most potent CCR 1 ligand ( MIP 1alpha > MPIF 1 > RANTES > or=MIP 1beta ) although the ligands differed in their efficacy as agonists . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Chemokines that bind CCR 1 ( MIP 1alpha , RANTES , and monocyte chemoattractant protein 3 [ MCP 3 ] ) were produced to varying degrees by murine OCs , OBs , and their precursors , and markedly increased by interleukin ( IL ) 1alpha and TNFalpha in differentiating OBs . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The chemokine macrophage inflammatory protein 1alpha ( MIP 1alpha ) and its receptor , CC chemokine receptor 1 ( CCR 1 ) , have been identified as crucial to the inflammatory response to PVM and hRSV and thus as elements to exploit for potential immunomodulatory control . ^^^ Biochemical blockade of MIP 1alpha signaling with the CCR 1 antagonist met RANTES prevents the inflammatory response to PVM and results in reduced morbidity and mortality when administered in conjunction with the antiviral agent ribavirin . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| However , the remaining expression of CCR 1 and CCR 5 correlated reciprocally with an ongoing unresponsiveness to the CCR 1 and CCR 5 agonists CCL3 / macrophage inflammatory protein 1alpha ( MIP 1alpha ) , CCL4 / MIP 1beta , and CCL5 / regulated on activation , normal T expressed and secreted ( RANTES ) , all chemokines binding to these two receptors . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| In both strains , we detected similarly increased expression of chemokines RANTES / CCL5 , macrophage inflammatory protein 1alpha ( MIP 1alpha ) / CCL3 , MIP 1beta / CCL4 , and MIP 2 , as well as chemokine receptors CCR 1 , CCR 2 , and CCR 5 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Chemokines that can bind to CCR 1 ( CCR 1 ligands ) , such as regulated on activation , normal T cell expressed and secreted ( RANTES ) and macrophage inflammatory protein 1alpha ( MIP 1alpha ) , were confirmed in the decidual tissues by RT PCR and immunohistochemistry . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The constitutive expression of these chemokines and the CC receptors was unaffected , whereas induced expression of MIP 1alpha , MIP 1beta , and 1 309 , and that of the CCR 1 receptor all involved in monocyte chemotaxis was significantly inhibited by MLIF . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The intracellular Ca ( 2+ ) concentration and the chemotactic activity increased in 9 CRA treated cells in response to the CCR 1 dependent chemokines Lkn 1 , MIP 1alpha , and RANTES , and the CCR 2 specific chemokine MCP 1 . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Antagonizing the interaction of MIP 1alpha with its receptor CC chemokine receptor 1 ( CCR 1 ) or signal transduction from CCR 1 may therefore prove to be effective as an antiinflammatory therapy on the ocular surface . . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| Increased expression of mRNA specific for various chemokines ( eotaxin , MIP 1alpha , MIP 2 ) and chemokine receptors ( CCR 1 , CCR 2 and CCR 5 ) was observed in the lungs of nonsensitized mice challenged with the spores . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| MIP 1alpha utilizes both CCR 1 and CCR 5 to induce osteoclast formation and increase adhesion of myeloma cells to marrow stromal cells . ^^^ Several chemokine receptors ( CCR 1 , CCR 5 , and CCR 9 ) mediate the effects of MIP 1alpha . ^^^ METHODS : We employed RT PCR analysis , neutralizing antibodies to CCR 1 and CCR 5 as well as a CCR 1 specific antagonist and OCL formation assays to identify the MIP 1alpha receptors involved in MIP 1alpha ' s effects on myeloma cells and OCL formation . ^^^ Neutralizing antibodies to CCR 1 or CCR 5 inhibited MIP 1alpha induced OCL formation . ^^^ Furthermore , monocyte chemotactic protein 3 ( MCP 3 ) , which binds CCR 1 but not CCR 5 and the CCR 1 specific antagonist , BX 471 , markedly inhibited OCL formation stimulated with MIP 1alpha . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The recruitment of major histocompatibility complex ( MHC ) class 2 ( + ) LC was significantly suppressed in CCR 5 ( / ) mice and blockade of RANTES and MIP 1beta , but not in CCR 1 ( / ) , CCR 2 ( / ) / MIP 1alpha ( / ) , or MIP 1alpha ( / ) mice . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| One such chemokine receptor is CCR 1 which is particularly responsive to the C C chemokines RANTES and MIP 1alpha . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| RESULTS : CCR 1 , a receptor for macrophage inflammatory protein 1alpha ( MIP 1alpha ) / CCL3 and RANTES / CCL5 , exhibited high constitutive expression on macrophages in AIA . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| The aim of the study was to compare the immunoexpression of monocyte chemoattractant protein 1 ( MCP 1 ) , monocyte inflammatory protein 1alpha ( MIP 1alpha ) , Regulated upon Activation Normal T cell Expressed and Secreted ( RANTES ) and CC chemokine receptors : CCR 1 and CCR 5 in renal biopsy specimens in IgA nephropathy ( IgAN ) and in non IgA mesangial proliferative glomerulonephritis ( MesProGN ) , and to find any relationships between the immunoexpression of chemokines and chemokine receptors and renal interstitial lesions . ^^^ |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P10147 and P32246 |
Pubmed |
SVM Score :0.0 |
| NA |
|