| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.97957226 |
| Association of PDGFR with PLC gamma 1 and RasGAP followed by their tyrosine phosphorylation failed , however , to activate PLC gamma 1 , protein kinase C ( PKC ) , and Ras . 0.97957226^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| Upon ligand induced tyrosine phosphorylation , the platelet derived growth factor ( PDGF ) receptor ( PDGFR ) beta subunit associates with PLC gamma 1 , RasGAP , P13K , and a 64 kd protein . ^^^ F 5 PDGFR failed to activate PLC gamma 1 , P13K , or Ras and was unable to trigger DNA synthesis . ^^^ Permitting association of F 5 PDGFR with either PLC gamma 1 or P13K restored Ras activation and a mitogenic response . ^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| To identify the phosphotyrosine involved in association of phospholipase C ( PLC ) gamma 1 with the beta platelet derived growth factor receptor ( PDGFR ) , and to investigate which contiguous residues confer specificity for PLC gamma 1 , phosphotyrosine containing glutathione S transferase ( GST ) fusion proteins possessing different regions of the beta PDGFR were incubated with lysates of Rat 2 cells that overexpress PLC gamma 1 . ^^^ The phosphorylated C terminal tail of the PDGFR bound PLC gamma 1 , but did not associate with phosphatidylinositol ( PI ) 3 ' kinase or GTPase activating protein ( GAP ) . ^^^ Substitution of the proline at the +3 position relative to Tyr 1021 with methionine ( Y1021IIP > Y1021IIM ) in the phosphorylated PDGFR tail did not alter PLC gamma 1 association , but conferred binding activity towards PI 3 ' kinase , indicating that this residue is critical in discriminating between PLC gamma 1 and PI 3 ' kinase . ^^^ Progressive conversion of the three residues C terminal to Tyr 1021 to the consensus for PI 3 ' kinase binding ( YMDM ) allowed PI 3 ' kinase association , but did not block PLC gamma 1 binding , suggesting that additional residues other than the three residues immediately following the phosphotyrosine may contribute to the association of PLC gamma 1 with the PDGFR . ^^^ These results indicate that phosphorylation at Tyr 1021 in the tail of the PDGFR creates a specific binding site for PLC gamma 1 . ^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| PDGF receptor ( PDGFR ) , protein kinase C alpha ( PKC alpha ) , phospholipase C gamma 1 ( PLC gamma 1 ) , and PLD 1 were examined by Western blot analysis . ^^^ The protein levels of PKC alpha and PLC gamma 1 were unchanged , but those of PLD 1 and PDGFR were reduced with age . ^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| Here we show that functional phospholipase C gamma 1 ( PLC gamma 1 ) is required for c Abl activation by PDGFR . ^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| The signal transduction protein phospholipase C gamma 1 ( PLC gamma 1 ) is activated when its C terminal SH 2 domain ( PLCC ) binds the phosphorylated Tyr 1021 site ( pTyr 1021 ) in the beta platelet derived growth factor receptor ( PDGFR ) . ^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| Expression of a partially activated A Raf mutant resulted in decreased tyrosine phosphorylation of the PDGFR , specifically on Y 857 ( autophosphorylation site ) and Y 1021 ( phospholipase Cgamma 1 ( PLCgamma 1 ) binding site ) , but not the binding sites for other signalling proteins ( Nck , phosphatidylinositol 3 ' kinase ( PI3K ) , RasGAP , Grb 2 , SHP ) . ^^^ Thus , A Raf can regulate PLCgamma 1 signalling via a PDGFR dependent mechanism and may also regulate PI3K signalling via a PDGFR independent mechanism . . ^^^ |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UFY1 and P09619 |
Pubmed |
SVM Score :0.0 |
| NA |
|