| Interacting proteins: P09619 and P22681 |
Pubmed |
SVM Score :0.0 |
| We identified platelet derived growth factor receptor alpha ( PDGFR alpha ) as one target of mutant Cbl induced deregulation . ^^^ In mutant Cbl transfectants , PDGFR alpha was hyperphosphorylated and constitutively complexed with a number of SH 2 domain containing proteins . ^^^ PDGFR alpha hyperphosphorylation and enhanced proliferation of mutant Cbl transfected NIH 3T3 cells were drastically reduced upon serum starvation , and PDGF AA substituted for the maintenance of these traits . ^^^ PDGF AA stimulation of serum starved Cbl transfectants induced the in vivo association of transfected Cbl proteins with PDGFR alpha . ^^^ In vitro , Cbl N directly bound to PDGFR alpha derived from PDGF AA stimulated cells but not to that from unstimulated cells , and this binding was abrogated by a point mutation ( G306E ) corresponding to a loss of function mutation in SLI 1 . ^^^ |
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| Interacting proteins: P09619 and P22681 |
Pubmed |
SVM Score :0.0 |
| A point mutation ( G306E ) that inactivates the tyrosine kinase binding domain in the N terminal transforming region of Cbl compromised the PDGF inducible tyrosine phosphorylation of Cbl although this mutant could still associate with the PDGFR . ^^^ More importantly , the G306E mutation abrogated the ability of Cbl to enhance the ligand induced ubiquitination and degradation of the PDGFR and to inhibit the PDGF dependent cell proliferation and protection from apoptosis . ^^^ These results demonstrate that Cbl can negatively regulate PDGFR dependent biological responses and that this function requires the conserved tyrosine kinase binding domain of Cbl . . ^^^ |
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| Interacting proteins: P09619 and P22681 |
Pubmed |
SVM Score :0.0 |
| TGF beta , c Cbl , and PDGFR alpha the in mammary stroma . ^^^ In addition , both TGF beta and Cbl regulated platelet derived growth factor receptor alpha ( PDGFR alpha ) expression in vivo and in isolated mammary fibroblasts . ^^^ The hypothesis that TGF beta mediates the levels of PDGFR alpha protein via regulation of c Cbl was tested . ^^^ We conclude that TGF beta regulates PDGFR alpha in the mammary stroma via a c Cbl independent mechanism . ^^^ |
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| Interacting proteins: P09619 and P22681 |
Pubmed |
SVM Score :0.0 |
| Low density lipoprotein receptor related protein 1 ( LRP 1 ) controls endocytosis and c CBL mediated ubiquitination of the platelet derived growth factor receptor beta ( PDGFR beta ) . ^^^ |
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