| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.66645013 |
| Solubilized bFGF was found as a complex with the 200 kD HSPG . 0.66645013^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.7664501 |
| The analysis of congenital nephropathies additionally indicates that also the HSPG side chains with their negative charges may be involved in certain diseases with filter impairment . 4 . ) Furthermore , HSPG serves as a binding site for growth factors , particularly for the basic fibroblast growth factor ( bFGF ) . 0.7664501^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.97047587 |
| The interaction between FGF 2 and proteoglycans was assayed by examining the binding of 125I FGF 2 to breast cancer cell cultures as well as to cationic membranes loaded with HSPG . 0.97047587^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.93653025 |
| The balance between intracellular FGF 2 and HSPG bound secreted FGF 2 is shown not to be controlled by the availability of cell surface HSPGs , indicating that the FGF 2 secretion machinery itself is rate limiting . . 0.93653025^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| A preliminary survey of several defined and affinity purified species of cell surface HSPG , including syndecan , fibroglycan , and glypican failed to identify natural HSPG that promote high affinity receptor binding of bFGF . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Our results indicate that 3 8 % of the total cellular content of bFGF is associated with glycosyl Pl anchored cell surface HSPG . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Cell surface heparan sulfate proteoglycans ( HSPG ) constitute relatively low affinity binding sites for bFGF . ^^^ Furthermore , binding of bFGF to cell surface HSPG is necessary for its binding to high affinity FGF receptors and for its mitogenic activity . ^^^ In addition , bFGF is bound to HSPG in the extracellular matrix ( ECM ) . ^^^ It has been suggested that bFGF may be sequestered in ECM as part of a stable insoluble FGF HSPG complex from which it is released by specific enzymatic mechanisms when needed for mitogenic activity . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycan ( HSPG ) is a major component of the muscle extracellular matrix and our previous study has shown that basic fibroblast growth factor ( bFGF ) , a member of the heparin binding growth factor ( HBGF ) family , and its receptor are present in Xenopus myotomal muscle during the period of synaptogenesis . ^^^ This exogenously applied bFGF could be largely removed by treatment of cultures with heparin , suggesting its association with HSPG at the cell surface . ( 5 ) An anti bFGF neutralizing antiserum significantly reduced the efficacy of the bead stimulation . ^^^ These data suggest that uncoated beads , which adhere to the cell surface and can mimic the cell substratum interaction , effect a local presentation of HBGFs , such as bFGF , residing with the HSPG to their membrane receptors , thereby locally activating receptor associated tyrosine kinases . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) binds to cell surface receptor ( CSR ) proteins and to heparan sulfate proteoglycans ( HSPG ) . ^^^ Basic fibroblast growth factor ( bFGF ) binds to cell surface receptor ( CSR ) proteins and to heparan sulfate proteoglycans ( HSPG ) . ^^^ Thus , the difference in Kd ' s appears to result from the faster rate at which bFGF is released from the HSPG sites compared to the CSR . ^^^ The `` high affinity ' ' binding of bFGF to intact cells probably refers only to a complex of bFGF with both CSR and HSPG , and not to the CSR alone . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| A simple procedure using human basic fibroblast growth factor ( FGF ) was utilized for the selection of COS cell mutants with defects in the biosynthesis or expression of heparan sulfate proteoglycan ( HSPG ) . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Alz 50 , a monoclonal antibody ( MAb ) to an abnormal form of tau and bFGF binding label mutually exclusive subpopulations of neurofibrillary tangles . bFGF binding is abolished by heparinase or heparitinase treatment and therefore is most likely based on the presence of HSPG . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We found that basic fibroblast growth factor ( bFGF ) binds to heparinase sensitive sites in NFTs . bFGF binding is due to a heparan sulfate proteoglycan ( HSPG ) immunocytochemically identified in NFTs . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Both HSPG species bind bFGF . 125I bFGF bound to BCE cell cultures is readily released by either heparinase or plasmin . ^^^ We propose that this plasminogen activator mediated proteolysis provides a mechanism for the release of biologically active bFGF HSPG complexes from the extracellular matrix and that bFGF release can be regulated by the balance between factors affecting the pericellular proteolytic activity . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The codistribution of both types of IRs with basic fibroblast growth factor ( bFGF , FGF 2 ) in neurons and astroglia supports the notion that heparan sulfate proteoglycans ( HSPG ) in the extracellular matrix may serve as a site for storage of bFGF and assist in the bFGF induced activation of the high affinity FGF receptors linked to astroglia and neurons in these discrete areas . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| A survey of defined species of cell surface and extracellular matrix heparan sulfate proteoglycans ( HSPG ) was performed in a search for cellular proteoglycans that can promote bFGF receptor binding and biological activity . ^^^ Of the various affinity purified HSPGs tested , perlecan , the large basement membrane HSPG , is found to induce high affinity binding of bFGF both to cells deficient in HS and to soluble FGF receptors . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| It is noteworthy that HSPG binding protects FGF 2 from denaturation and proteolytic degradation , provides a matrix bound or cell surface reservoir of this factor for the cells and is required for the activation of FGF high affinity receptors . ^^^ Using as model system L 6 myoblasts lacking endogenous FGF receptors ( FGFRs ) , we demonstrated that these cells internalize FGF 2 efficiently through an HSPG mediated pathway . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Biochemical studies have shown that basic fibroblast growth factor ( bFGF or FGF 2 ) is internalized by two pathways , after binding to either FGF tyrosine kinase receptors or to heparan sulfate proteoglycans ( HSPG ) . ^^^ To get insights on the HSPG mediated pathway , we have examined by electron microscopy the intracellular route of bFGF HRP , a monovalent conjugate of bFGF and horseradish peroxidase which was found to bind to HSPG only and was detectable by electron microscopy . bFGF HRP association to adult bovine aortic endothelial ( ABAE ) cells or baby hamster kidney ( BHK ) cells was inhibited by a high molar excess of native bFGF , a 2 M NaCl wash at neutral pH , heparin and heparan sulfate , but not by chondroitin 4 sulfate or chondroitin 6 sulfate . bFGF HRP was not able to displace [ 125I ] bFGF from its high affinity binding sites , and the dissociation constant of its binding to ABAE cells was estimated at 3 nM . ^^^ These results suggest that bFGF associated to HSPG can enter the cell via several pathways and follows mainly a degradative route . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycans ( HSPG ) are obligatory for receptor binding and mitogenic activity of basic fibroblast growth factor ( bFGF ) . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We had previously demonstrated that fragments > 6 mer can inhibit the interaction between cell surface heparan sulphate proteoglycan ( HSPG ) and bFGF , and bFGF induced proliferation of adrenocortical endothelial ( ACE ) cells . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycan ( HSPG ) , for example , associates with several matrix components such as fibronectin , laminin , and basic fibroblast growth factor . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The HSPG exactly co localizes with FGF 2 within the neuroepithelium of the embryonic mouse . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The present studies examined how 125I labeled basic fibroblast growth factor ( bFGF ) bound to high affinity receptors and with lower affinity to heparan sulfate proteoglycans ( HSPG ) of cultured immature rat Leydig cells was processed . ^^^ At time zero and at specific intervals over the next 6 h , the incubation medium was saved and cells washed to quantitate 125I bFGF released into the medium , associated with HSPG of the cell surface or extracellular matrix ( radioactivity released by washing cells with 2 M NaCl , pH 7 . 4 ) , associated with cell surface receptors ( radioactivity released by washing cells with 2 M NaCl , pH 4 . 0 ) or internalized ( radioactivity resistant to high salt and acid washes , and solubilized with 0 . 5 M NaOH ) . ^^^ Radioactivity released into the initial medium and the pooled washes was further divided into a trichloroacetic acid ( TCA ) precipitated form ( radioactivity precipitated by 10 % TCA ) and a TCA soluble form ( radioactivity remaining in the TCA supernatant ) . 125I bFGF associated with both HSPG and surface receptors declined progressively during the first 4 h of incubation before stabilizing when cells were transferred to 37 degrees C . ^^^ The majority of internalized 125I bFGF appeared to originate from the HSPG bound fraction as there was a greater decline in HSPG associated radioactivity and most of the increase in internalized radioactivity could be blocked by the inclusion of 10 micrograms / ml heparin ( which mainly blocks 125I bFGF binding to HSPG but not to high affinity receptors ) during the initial incubation with 125I bFGF for 2 h at 4 degrees C . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Cell surface expression of the basement membrane form of heparan sulfate proteoglycan ( HSPG ) , perlecan , basic fibroblast growth factor ( bFGF ) binding forms of heparan sulfate , and laminin has been examined by immunofluorescence during the attachment and outgrowth phases of mouse embryo development in vitro and in utero . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| A heparan sulfate proteoglycan ( HSPG ) from bovine cardiac plasma membrane was purified to homogeneity using either isoelectric focusing or anion exchange chromatography , followed by affinity chromatography on immobilized basic fibroblast growth factor ( bFGF ) . ^^^ We conclude that adult bovine myocardium contains a membrane associated bFGF binding heparan sulfate proteoglycan containing little or no chondroitin sulfate and that this HSPG may be distinct from those of the syndecan family of heparan sulfate proteoglycans . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The angiogenic factor , basic fibroblast growth factor ( bFGF ) , is sequestered and protected by binding to heparan sulfate proteoglycans ( HSPG ) in the subendothelial extracellular matrix ( ECM ) . ^^^ Exposure of ECM to thrombin , the final activation product of the clotting cascade , resulted in release of high molecular weight HSPG bFGF complex , as indicated by its immunoprecipitation with anti bFGF antibodies , susceptibility to degradation by bacterial heparinase , and inhibition of its mitogenic activity in the presence of neutralizing anti bFGF antibodies . ^^^ The ECM resident bFGF HSPG complex was not released by thrombin in the presence of hirudin or antithrombin 3 , or by catalytically blocked thrombin preparations . ^^^ This difference is attributed to heat stable bFGF HSPG complexes that are more readily released after heat treatment of the ECM and to activation and release of ECM resident transforming growth factor beta ( TGF beta ) activity . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| These proteoglycans were sensitive to heparinase , demonstrating that K 562 cells synthesize bFGF binding HSPG . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Fibroblast growth factor 1 ( FGF 1 ) and 2 ( FGF 2 ) bind to two classes of receptors : the high affinity receptors , a family of four known transmembrane tyrosine kinases ( FGF R 1 R4 ) , and the low affinity receptors , cell surface and basement membrane heparan sulfate proteoglycan ( HSPG ) . ^^^ This change in the capacity of FGF 2 to bind to FGF R 1 and to HSPG was not due to a switch of the IIIc splice form of FGF R 1 to the IIIb splice form since the exon IIIc was the most predominant splice form of FGF R 1 during RPE cell cultures . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Our results showed that MCF 7 cells were unsensitive to FGF 2 after enzymatic degradation of heparin sulfate proteoglycans ( HSPG ) by heparinase . ^^^ The sulphated glycosaminoglycans which were critical in FGF 2 induced proliferation were strictly HSPG , as an addition of heparin in cell culture medium can restore FGF 2 mitogenic activity . ^^^ These results provide clear evidence for the critical role of HSPG in FGF 2 induced proliferation on MCF 7 breast cancer cells . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We have investigated the interaction of basic fibroblast growth factor ( bFGF ) with its receptors and heparan sulfate proteoglycans ( HSPG ) . ^^^ It has been suggested that in the absence of HSPG , cells are not able to bind bFGF or respond to treatment with bFGF . ^^^ We found that bFGF was able to bind , be internalized , and stimulate DNA synthesis in the absence of HSPG in a dose dependent manner . bFGF bound to its receptors on chlorate treated cells with a lower apparent affinity and no change in receptor number . ^^^ Endocytotic rate constants ( ke ) for chlorate treated and control cells were ke = 0 . 078 + / 0 . 022 min 1 and ke = 0 . 043 + / 0 . 012 min 1 , respectively , suggesting that the process of bFGF internalization is not dramatically altered by HSPG . bFGF stimulated DNA synthesis to the same maximal level under both conditions , but chlorate treated cells were significantly less responsive at low bFGF doses ( approximately 10 fold increase in ED 50 ) . ^^^ Although the presence of HSPG on the cell surface increases the affinity of bFGF for its receptors , our observations suggest that HSPG are not `` absolutely ' ' required for binding , internalization , or stimulation of mitogenic activity . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Bovine coronary SMCs ( cSMCs ) express bFGF , bFGF receptor ( FGF R 1 ) , and cell membrane integrated proteoheparan sulfate ( HSPG ) . ^^^ Trypsinization generates soluble forms of the complex of bFGF with the ectodomains of the bFGF receptor and cell membrane integrated HSPG in the pericellular compartment , thus allowing quantification of pericellular bFGF by a highly specific enzyme immunoassay . ^^^ The heparin induced increase in cellular protein content includes a 60 % to 100 % increase in the expression of pericellular bFGF , FGF R 1 , and cell membrane integrated HSPG . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| In order to obtain an insight into morphogenetic processes such as angiogenesis , cell proliferation , and tissue remodeling we have studied the localization of basic fibroblast growth factor ( bFGF ) and heparan sulfate proteoglycan ( HSPG ) in the human placenta by immunohistochemistry . ^^^ Positive reaction product for bFGF is found mainly in the villous trophoblastic covering and for HSPG in the villous basement membranes . ^^^ HSPG and bFGF are codistributed in the distal half of the villous stroma in the mesenchymal villi . ^^^ In cell islands and cell columns , bFGF is detectable in the cytoplasm of the extravillous cytotrophoblastic cells , whereas HSPG is localized between the extravillous cytotrophoblastic cells and in their cytoplasm . ^^^ HSPG bFGF codistribution in term placenta is confined to the walls of fetal vessels and to some extravillous cytotrophoblastic cells in the basal plate . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Using specific antibodies , we compared the immunolocalization of the vascular heparan sulfate proteoglycan ( HSPG ) binding protein vitronectin ( VN ) , HSPG , basic fibroblast growth factor , and collagen type 1 in retinae prepared from 11 month old diabetic and nondiabetic Wistar rats . ^^^ In normal rats , VN immunostaining was prominent in vascularized parts of the inner retina and colocalized with HSPG , which itself showed costaining with basic fibroblast growth factor in the extracellular matrix , vascular walls , and the inner limiting membrane . ^^^ In contrast , the recognition of VN , HSPG , and basic fibroblast growth factor in diabetic retinae was greatly reduced in the inner limiting membrane and the extracellular matrix , where increased immunoreactive AGE colocalizing with VN were detectable . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Heparan sulphate proteoglycan ( HSPG ) , which is composed of heparan sulphate glycosaminoglycan ( HSGAG ) , has also been shown to play an important role in the pathogenesis of tissue overgrowth by enhancing the functions of bFGF . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| HSPG may play some important roles in the regeneration of liver by interacting with various growth factors such as bFGF and HB EGF . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We have examined the importance of cell surface heparan sulfate proteoglycans ( HSPG ) in fibroblast growth factor ( FGF ) signaling . 3T3 cells grown under conventional conditions were much more sensitive to FGF 2 compared to FGF 1 . ^^^ However , cells were equally sensitive to FGF 1 and FGF 2 using conditions which reduced the effect of endogenous HSPG . ^^^ Glycosaminoglycan chains or core protein fragments derived from the HSPG failed to show the same high apparent affinity for FGF 2 , suggesting that an intact proteoglycan structure was important for the high FGF 2 affinity . ^^^ Addition of HSPG ectodomains , isolated from cultured 3T3 cells or produced as recombinant molecules , to chlorate treated cultures of 3T3 cells inhibited the mitogenic activity of FGF 2 and eliminated the effect of heparin as a potentiator of either growth factor . ^^^ These results support the idea that the cell associated HSPG is an integral component of the FGF signaling system and in 3T3 cells contributes to the increased sensitivity of these cells to FGF 2 compared to FGF 1 . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Taken together with the morphological evidence on autoradiography , it seems most likely that the injected trafermin could be inactivated in sinusoidal endothelial cells , probably through a well known internalization mechanism of the basic fibroblast growth factor HSPG complex . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Heparan sulfate proteoglycans ( HSPG ) have been shown to be involved in the activation of tyrosine kinase receptors by basic fibroblasts growth factor ( bFGF ) , a strong inhibitor of skeletal muscle differentiation . ^^^ All these results suggests that perlecan , a HSPG that binds myogenic inhibitory bFGF , normally associated with basement membranes in adult tissues is present on the surface of myoblasts and its expression is down regulated during skeletal muscle differentiation . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The released Tat binds to HSPG through its basic region and this determines its storage into the ECM , as occurs for bFGF . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) is a potent mitogen for vascular smooth muscle cells ( VSMC ) and has been implicated in a number of vascular disorders . bFGF interacts with high affinity receptors and heparan sulfate proteoglycans ( HSPG ) at the cell surface . ^^^ Basic fibroblast growth factor ( bFGF ) is a potent mitogen for vascular smooth muscle cells ( VSMC ) and has been implicated in a number of vascular disorders . bFGF interacts with high affinity receptors and heparan sulfate proteoglycans ( HSPG ) at the cell surface . ^^^ HSPG have been demonstrated to enhance bFGF binding to its receptors , yet no known role for HSPG in modulating postbinding events has been identified . ^^^ In the present study , we analyzed bFGF internalization , intracellular distribution , degradation , and stimulation of DNA synthesis within native and HSPG deficient VSMC . ^^^ Analysis of the accumulation of internalized bFGF within cytoplasmic and nuclear fractions of native and HSPG deficient VSMC showed striking differences . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Modification of HSPG induced by TGFbeta 1 or NaB treatments in normal and breast cancer epithelial cells resulted in an increase in 125I fibroblast growth factor 2 ( FGF 2 ) binding on HSPG . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Incubation of organ sections with 2 M NaCl or heparin eluted all the radioactivity , indicating that labeling was due to FGF 2 heparan sulfate proteoglycan ( HSPG ) interactions . ^^^ All FGF 2 catabolic fragments bound heparin , demonstrating the preservation of their HSPG binding site during the in vivo intracellular catabolism of FGF 2 . ^^^ This study shows for the first time that exogenous FGF 2 is cleared by HSPG cellular internalization and catabolism without inducing the activation of FGFRs within at least five organs in vivo , which strongly suggests that the HSPG dependent internalization and catabolism pathway may control the in vivo bioavailability of FGF2 . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Isolated heparan sulfate proteoglycan ( HSPG ) from liver cells in cell free complexes with FGFR 4 restored the specificity for FGF 1 and supported the binding of both FGF 1 and FGF 2 when complexed with FGFR 1 . ^^^ In contrast , FGF 2 bound equally well to complexes of both FGFR 1 and FGFR 4 formed with endothelial cell derived HSPG , but the endothelial HSPG was deficient for the binding of FGF 1 to both FGFR complexes . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| However , these antibodies appear to target preferentially different domains within the FGF 2 molecule , and therefore differentially influence the interactions of FGF 2 with its low and high affinity receptors . 254F1 is a more effective inhibitor of the high affinity , receptor tyrosine kinase binding site , whereas 256A12 appears to be a better inhibitor of the low affinity , HSPG interactions . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| In addition , we demonstrate this macrophage HSPG selectively binds the macrophage derived growth factors FGF 2 , vascular endothelial growth factor and heparin binding EGF and can present FGF 2 in a form that transactivates receptor bearing BaF 32 cells . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Ligand binding studies indicated that elastase treatment decreased total binding of ( 125 ) 1 bFGF to the cell surface and affected both fibroblast growth factor receptor and heparan sulfate proteoglycan ( HSPG ) binding sites . ^^^ These findings indicate that elastase mediated HSPG release from fibroblast matrices reduces the effective affinity of bFGF for its receptor . ^^^ Collectively , these studies suggest that HSPG co receptors are important mediators of the pulmonary fibroblast response to elastase treatment and that bFGF , HSPG , and other elastase released entities play an important role in the response of the lung to chronic injury . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We evaluated the expression of a select panel of growth factors and their receptors , including fibroblast growth factor 1 ( FGF 1 ) , fibroblast growth factor 2 ( FGF 2 ) , platelet derived growth factor ( PDGF ) , FGF receptor 1 ( FGF R 1 ) , FGF receptor 3 ( FGF R 3 ) , FGF receptor 4 ( FGF R 4 ) , PDGF receptor alpha ( PDGF Ralpha ) , PDGF receptor beta ( PDGF Rbeta ) , and heparan sulfate proteoglycan ( HSPG ) , in muscle biopsy specimens from nine facioscapulohumeral muscular dystrophy ( FSHD ) patients using immunohistochemistry . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The interaction of fibroblast growth factor 2 ( FGF 2 ) with heparan sulfate proteoglycans ( HSPG ) has been demonstrated to enhance receptor binding and alter the intracellular distribution of internalized FGF 2 . ^^^ In the present study , the intracellular fate of FGF 2 was analyzed in vascular smooth muscle cells ( VSMC ) under native and HSPG deficient conditions . ^^^ The kinetic analysis indicated that the greatest differences between native and HSPG deficient VSMC was in the formation of LMW and further suggested that these FGF 2 products appear to represent a stable subpool of internal FGF 2 that is favored in cells that contain HSPG . ^^^ Thus , HSPG might function as a cellular switch between immediate and prolonged signal activation by heparin binding growth factors such as FGF 2 . ^^^ In the absence of HSPG , FGF 2 can interact with and activate its receptor , yet in the presence of HSPG , FGF 2 might be able to mediate prolonged or unique biological responses through intracellular processes . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Fibroblast growth factor 2 ( FGF 2 ) is one of the best characterized of the heparin binding growth factors and it has been shown experimentally that heparin regulation of FGF 2 activity is dependent on the level of cell HSPG and the concentration of heparin . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the HSPG receptor for FGF 2 not only influences the outcome of FGF 2 signaling , e . g . cell proliferation , but importantly regulates the immediate early signals generated by this growth factor . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Here , we evaluated the effects of different heparan sulfate proteoglycan ( HSPG ) expressing cell lines and HSPGs derived from these cells on FGF 2 induced FGFR 1 phosphorylation in heparan sulfate negative BaF 3 cells . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| In this study , we have focused on the role of FGF 2 , which requires heparan sulfate proteoglycans ( HSPG ) as cofactors for binding and activation of its cell surface tyrosine kinase receptor . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Five micrometer sections cut from paraffin blocks were immunolabeled for collagen , heparan sulfate proteoglycans ( HSPG ) , FGF 2 , TGFbeta , and VEGF . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| This report shows that the bFGF regulation of their expression specifically depends on the nature of HSPG and of the Sertoli cell developmental stage . ^^^ In conclusion , HSPG are partners and the target of bFGF in rat Sertoli cells . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| However , it fully retains the FGF 2 binding ability of the original heparin , as shown by its capacity to protect FGF 2 from trypsin cleavage and to prevent the formation of heparan sulfate proteoglycan ( HSPG ) / FGF2 / FGFR1 ternary complexes . ^^^ However , when compared to heparin it showed a reduced capacity to induce FGF 2 dimerization and to favor the interaction of [ 125I ] FGF 2 with FGFR 1 in HSPG deficient , FGFR 1 transfected CHO cells . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Moreover , the heparin resistant binding of [ 125I ] bFGF in TM BBB 4 cells was significantly reduced by 50 % following treatment with sodium chlorate , suggesting the loss of perlecan ( a core protein of heparan sulfate proteoglycan , HSPG ) from the extracellular matrix of the cells . ^^^ RT PCR analysis revealed that HSPG mRNA and FGFR 1 and FGFR 2 ( tyrosine kinase receptors for bFGF ) mRNA are expressed in TM BBB 4 cells . ^^^ These results suggest that bFGF is internalized via HSPG , which is expressed on the abluminal membrane of the BBB . ^^^ HSPG at the BBB may play a role in maintaining the BBB function due to acceptance of the bFGF secreted from astrocytes . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Increased amounts of HSPG were present in the dermal nodules of scleromyxedema compared to adjacent normal dermis and these bound FGF 2 , immobilized the soluble receptor protein FGFR 1 and , therefore , formed a ternary complex composed of HSPG , FGF 2 and FGFR 1 in vitro . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We report here that heparan sulfate proteoglycan ( HSPG ) was found in bovine aqueous humor and that it directly inhibits binding of basic fibroblast growth factor and vascular endothelial growth factor to cell surface heparan sulfate . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Satellite cells that differ in proliferation rates differ in responsiveness to FGF 2 , and in the expression of FGF 2 , FGF receptor 1 , and HSPG . . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| One possible role for the nuclear localization of HSPG is to function as a shuttle for the nuclear transport of heparin binding growth factors , such as basic fibroblast growth factor ( FGF 2 ) . ^^^ Furthermore , pre treatment with heparinase 3 decreased nuclear FGF 2 , and CHO cells defective in the ability to properly synthesize heparan sulfate chains showed reduced nuclear FGF 2 indicating that the heparan sulfate chains of HSPG are critical for this process . ^^^ HSPG signaling , particularly through the cytoplasmic tails of syndecans , was investigated as a potential mechanism for the nuclear localization of FGF 2 . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( FGF 2 ) activates its high affinity receptors ( FGFRs ) but also acts through interaction with heparan sulfate proteoglycans ( HSPG ) . ^^^ Basic fibroblast growth factor ( FGF 2 ) activates its high affinity receptors ( FGFRs ) but also acts through interaction with heparan sulfate proteoglycans ( HSPG ) . ^^^ Overall , our data suggest that FGF 2 stimulates alpha 6 over expression in HUVEC , through HSPG but independently from FGFR , and that LMWF ( or LMWH ) modulates this interaction . ^^^ |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Moreover , using heparan sulfate proteoglycan ( HSPG ) deficient myeloid cells and porcine aortic endothelial cells ( PAECs ) expressing chimeric FGFR 1 , we show that antagonism of FGFR 1 mediated DNA synthesis and MAPK activation by SPARC does not require the presence of cell surface , low affinity FGF 2 receptors , but can be mediated by an intracellular mechanism that is independent of an interaction with the extracellular ligand binding domain of FGFR 1 . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Interestingly , comparison of sensorgrams of sequential injections of FGF 2 and FGFR 1 and equimolar FGF 2 FGFR1 injections onto a heparin neoproteoglycan surface demonstrates that FGF 2 dramatically enhances the association of FGFR 1 with heparin and leads us to propose a model for the stepwise assembly of a ternary FGF FGFR HSPG complex . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Basic fibroblast growth factor ( bFGF ) , its receptor FGF R 1 , and coreceptor heparansulfate proteoglycan ( HSPG ) are important components involved in this angiogenic process . ^^^ Basic fibroblast growth factor ( bFGF ) , its receptor FGF R 1 , and coreceptor heparansulfate proteoglycan ( HSPG ) are important components involved in this angiogenic process . ^^^ Ang 2 induces an AT 1 receptor dependent expression of bFGF and also upregulates the FGF R 1 and HSPG expression which is suppressed by losartan , the AT 1 receptor blocker . ^^^ AT 1 receptor signaling which is characterized by phosphorylation of p 42 mitogen activated protein kinase ( MAPK ) is involved in Ang 2 induced bFGF , FGF R 1 and HSPG upregulation and DNA synthesis in human cSMC . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The most universal angiogenic cytokines ( VEGF , bFGF , HGF ) are all heparin binding proteins , the function of which is dependent on cell surface heparan sulfate proteoglycans ( HSPG ) . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : FGF 2 was transferred to FGFR 1 through binding to HSPG , which is characteristically expressed on RASFs , resulting in RANKL and ICAM 1 mediated maturation of osteoclasts via ERK activation . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The spatiotemporal expression of syndecan 3 during feather development suggests that this cell surface HSPG may be involved in the response of competent embryonic skin dermis to FGF 2 . . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| In contrast , initiated progenitors did not exit the cell cycle when stimulated with PDGF and / or FGF 2 . cDNA oligoarray analysis and RT PCR verification showed an early upregulation / induction of growth factor / receptors , PDGF A , PDGFR beta , IGFR 1 , IGF 1 and 2 , IL 6 , MEGF 5 , FRAG 1 , IRS 2 , HSPG , and FGFR 1 , followed by a late increase in the expression IGFBP 6 , PDGF alpha , FGFR 4A , c / ERB A , and FGFR 4 , 2 , and 1 during the tumorigenic progression . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| In particular , heparin binding growth factors , such as fibroblast growth factor 2 ( FGF 2 ) , bind to heparan sulfate proteoglycans ( HSPG ) on the cell surface and within the extracellular matrix ( ECM ) , and these molecules have been viewed as accessory co receptors serving to facilitate tyrosine kinase receptor binding . ^^^ Recent studies , however , have indicated that HSPG can directly participate in signal transduction in response to FGF 2 binding . ^^^ We find that FGF 2 binding to its receptor correlates well with Erk1 / 2 activation and that HSPG can modulate this response through its ability to stabilize these ligand receptor complexes . ^^^ Moreover , we also observed that FGF 2 binding to HSPG correlates strongly with Erk1 / 2 activation under conditions where there is a loss of receptor activity , and we demonstrate that the relative amounts of signaling and non signaling HSPG on the cell surface , as well as the presence of competing HSPG in the ECM , can impact the signal potential via this pathway . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Glioma cell expression of the cell surface HSPG glypican 1 closely mirrored the FGF 2 coactivator activity . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| The levels of mRNA expression were regulated by FGF 2 : in late progenitors , FGF 2 induced a doubling of the mRNA levels of syndecan 2 , 3 , and 4 , while those for syndecan 1 and glypican remained unaffected ; in mature OLs , the levels of syndecan 1 mRNA were up regulated , the levels of syndecan 2 and 4 and glypican were down regulated . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| Higher syndecan 2 expression changed cell morphology and increased spreading and adhesion in these cells and proliferation induced by FCS and FGF 2 . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| We examined the intracellular dynamics of FGF 2 , FGFR 1 and the HSPGs syndecan 2 and 3 , glypican 1 and 2 , and perlecan in neurones and glia in and around adult rat cerebral wounds . ^^^ |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P34741 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|