| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.89026947 |
| These results indicate that PF 4 interacts with FGF 2 by complex formation , inhibiting FGF 2 dimerization , binding to FGF receptors , and internalization . 0.89026947^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.57371919 |
| The ability of PF 4 to bind simultaneously to both FGF 2 and HS was assessed using affinity gel chromatography . 0.57371919^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Intracerebroventricular ( i . c . v . ) microinfusion of epidermal growth factor ( EGF ) , basic fibroblast growth factor ( bFGF ) and platelet factor 4 ( PF 4 ) suppressed 2 h and nighttime food intake in rats . ^^^ I . c . v . infusion of bovine serum albumin ( BSA ) , nerve growth factor ( NGF ) , or inactivated EGF , bFGF and PF 4 had no effect . ^^^ Intraperitoneal ( i . p . ) administration of EGF , bFGF , PF 4 and PDGF in doses equivalent to or higher than those administered centrally had no effect . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Although secreted CXCL 4 and CXCL4L1 differ in only three amino acids , CXCL4L1 was more potent in inhibiting chemotaxis of human microvascular endothelial cells toward interleukin 8 ( IL 8 ) / CXCL8 or basic fibroblast growth factor ( bFGF ) . ^^^ In vivo , CXCL4L1 was also more effective than CXCL 4 in inhibiting bFGF induced angiogenesis in rat corneas . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| CT 102 APST ( Curative Technologies , Setauket , NY ) was prepared from homologous platelets and contained multiple growth factors including PDGF , PDAF , EGF , PF 4 , TGF beta , aFGF , and bFGF . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Platelet factor 4 ( PF 4 ) blocked the binding of basic fibroblast growth factor ( bFGF ) to the plasma membrane receptor . ^^^ Five micrograms / ml of PF 4 completely blocked the specific binding of bFGF to the receptor of NIH 3T3 cells . ^^^ The inhibition reached maximum at 5 micrograms / ml of PF 4 , where the binding of bFGF to the receptor was completely blocked . . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| HRGs from both chicken and human , and human PF 4 , were demonstrated to compete with each other and with acidic FGF ( aFGF ) and basic FGF ( bFGF ) for binding to BALB / c 3T3 cell surface HSPGs , whereas ATIII did not compete . ^^^ Thus , HRG , PF 4 , aFGF , and bFGF all interact with the same HS chains on the 3T3 cell surface , either binding to the same or binding to adjacent saccharide sequences on the chains . ^^^ In terms of their relative binding affinity for cell surface HSPGs , the hierarchy was shown to be PF 4 > or = bFGF > aFGF = cHRG > hHRG . ^^^ Indeed , both HRG and PF 4 , at physiological concentrations , were shown to effectively inhibit the binding of 125I aFGF and 125I bFGF to ECM . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| We tested the ability of platelet factor 4 ( PF 4 ) and other platelet heparin binding proteins to modulate bFGF stimulated [ 3H ] thymidine incorporation into fibroblasts . ^^^ The supernatant of thrombin stimulated platelets contained an inhibitor of bFGF induced mitogenesis ; this activity coeluted with PF 4 upon gel filtration , heparin agarose , and ion exchange chromatography . ^^^ PF 4 inhibited the activity of 5 pM bFGF with 50 % inhibitory concentration of 75 nM . ^^^ Inhibition of bFGF activity by PF 4 could be overcome by exogenous heparin or chondroitin 4 sulfate , suggesting that inhibition of mitogenesis is caused by binding of PF 4 to cell surface glycosaminoglycans . ^^^ These results indicate that an important role of PF 4 released at sites of vascular injury and platelet activation is to control cellular proliferation caused by the release of bFGF from ruptured cells . . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Platelet factor 4 ( PF 4 ) blocks the binding of basic fibroblast growth factor ( bFGF ) to its receptor . ^^^ In the present study , we constructed carboxyl terminal fragments , which represent the heparin binding region of the PF 4 molecule , and examined whether these synthetic peptides retain the blocking effects on the receptor binding of bFGF . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Binding of 125I labeled PF 4 was partially inhibited by the presence of increasing concentrations of protamine sulfate and basic fibroblast growth factor . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Here , we present data to show that the angiogenesis inhibitor platelet factor 4 ( PF 4 ) is able to prevent basic fibroblast growth factor ( bFGF ) induced down regulation of intercellular adhesion molecule 1 ( ICAM 1 ) . ^^^ Furthermore , PF 4 restores ICAM 1 expression following bFGF induced down regulation of ICAM 1 . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| In this study , we examined in detail the interaction of platelet factor 4 ( PF 4 ) with fibroblast growth factor 2 ( FGF 2 ) and vascular endothelial growth factor ( VEGF ) and the effect of PF 4 derived synthetic peptides . ^^^ This is based on the following observations : PF 4 peptide 47 70 inhibited FGF 2 or VEGF binding to endothelial cells ; it inhibited FGF 2 or VEGF binding to FGFRs or VEGFRs in heparan sulfate deficient CHO cells transfected with FGFR 1 ( CHOFGFR 1 ) or VEGFR 2 ( CHOmVEGFR 2 ) cDNA ; it blocked proliferation or tube formation in three dimensional angiogenesis assays ; and , finally , it competed with the direct association of ( 125 ) 1 PF 4 with FGF 2 or VEGF , respectively , and inhibited heparin induced FGF 2 dimerization . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| We have demonstrated that heparanase and platelet factor 4 ( PF 4 ) , released from activated platelets at the site of injury , liberate bFGF from the extracellular matrix ( ECM ) of vascular smooth muscle cells ( SMC ) , resulting in the induction of SMC proliferation and migration . ^^^ Increases in proliferation and migration were inhibited by treatment with a bFGF neutralizing antibody , suggesting that proliferation and migration in response to heparanase or PF 4 are mediated by bFGF activation . ^^^ Platelet degranulation products , such as heparanase and PF 4 , may liberate bFGF from extracellular sequestration , activating the growth factor and inducing the SMC proliferation and migration that contribute to the wound healing response following vascular injury . . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| The effect of platelet factor 4 ( PF 4 ) on myoblast cultures with or without basic fibroblast growth factor ( bFGF ) or other growth factors was investigated in the present in vitro experiments , with reference to bFGF binding to myoblast membrane fraction . ^^^ When bFGF and PF 4 were simultaneously added to the culture medium , PF 4 abolished the facilitatory effects of bFGF on myogenesis . ^^^ The real time biospecific interaction analysis ( BLA ) core system showed that the myoblast membrane fraction at 1 day after cultivation contains bFGF binding elements which are blocked by PF 4 in a dose dependent manner . ^^^ These findings suggest that : 1 ) PF 4 inhibits myoblast proliferation and myotube formation only for a limited initial period of cultivation , possibly because of the time dependent down regulation of high affinity bFGF receptors : and 2 ) PF 4 may be used as a tool to investigate the function of endogenous heparin binding growth factors upregulated transiently at a certain developmental stage or in case of tissue damage and repair , even though it is not monospecific to bFGF . . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| The heparin binding domains of PF 4 have been reported to prevent vascular endothelial growth factor 165 ( VEGF ( 165 ) ) and fibroblast growth factor 2 ( FGF 2 ) from interacting with their receptors . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| The viscoelastic properties of the hydrogels can be altered via variations in the ratio of LMWH to PF 4 ZIP . bFGF release from these gels have also been investigated . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Platelet factor 4 , which blocks the binding of bFGF to its receptor , inhibited network formation . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| We investigated the effects of PF 4 on the intracellular signal transduction induced by basic fibroblast growth factor ( FGF 2 ) . ^^^ We found that PF 4 ( 10 microg / mL ) inhibited the FGF 2 induced proliferation of adrenal cortex capillary endothelial ( ACE ) cells . ^^^ Surprisingly , PF 4 did not affect FGF 2 induced Akt phosphorylation . ^^^ This suggests that PF 4 disrupts FGF 2 signaling via an intracellular mechanism of inhibition . ^^^ To exclude the possibility that PF 4 inhibited the binding of FGF 2 to only one FGF receptor , preferentially activating the ERK pathway , we investigated the effect of PF 4 on FGF 2 induced ERK and Akt phosphorylation , using mutant heparan sulfate deficient Chinese hamster ovary cells transfected with the FGF R 1 cDNA . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| In contrast , the CXC chemokines lacking the ELR motif , platelet factor 4 , interferon gamma inducible protein 10 , and monokine induced by gamma interferon , not only failed to induce significant in vitro endothelial cell chemotaxis or in vivo corneal neovascularization but were found to be potent angiostatic factors in the presence of either ELR CXC chemokines or the unrelated angiogenic factor , basic fibroblast growth factor . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Protective effect of basic fibroblast growth factor heparin and neurotoxic effect of platelet factor 4 on ischemic neuronal loss and learning disability in gerbils . ^^^ Platelet factor 4 , which has a potent affinity for heparin , has been shown to inhibit the binding of basic fibroblast growth factor to the cell surface receptor and to counteract the biological activities of basic fibroblast growth factor in certain peripheral tissues . ^^^ In the present in vitro [ 125I ] basic fibroblast growth factor binding experiments , platelet factor 4 consistently inhibited the binding of iodinated basic fibroblast growth factor to cell membranes of the gerbil hippocampus . ^^^ To investigate the in vivo function of endogenous basic fibroblast growth factor and / or basic fibroblast growth factor receptor possibly activated in the ischemic gerbil brain , we infused platelet factor 4 continuously into the left lateral ventricle with an osmotic minipump . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Three angiogenesis inhibitors , alpha interferon , TNP 470 , and platelet factor 4 , inhibited VEGF and bFGF induced cell migration . ^^^ |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Consistent with these results , heparin , suramin , protamine sulphate and platelet factor 4 inhibited bFGF induced proliferation of human aortic smooth muscle cells . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| Effects of basic fibroblast growth factor ( bFGF ) neutralizing antibody and platelet factor 4 on facial nerve regeneration . ^^^ The present study was designed to examine the role of endogenous bFGF , rather than exogenous bFGF in the regenerative process of the transected facial nerve of guinea pig , by using the so called silicone tubulization model which enabled us to bridge the transected facial nerve with a silicone tube and to inject into the tube bFGF neutralizing antibody , normal IgG , saline , or platelet factor 4 ( an antagonist for bFGF receptor ) . ^^^ |
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| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02776 and P09038 |
Pubmed |
SVM Score :0.0 |
| NA |
|