| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.57965542 |
| Radiolabeled filensin binds specifically to lens vimentin under isotonic conditions , as demonstrated by affinity chromatography and ligand blotting assays . 0.57965542^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| Calcium overload , induced by ionomycin in artificial aqueous humor with 1mM calcium , resulted in lens opacification and degradation of cytoskeletal proteins including spectrin , filensin , and vimentin . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| In vitro studies on the assembly properties of the lens proteins CP 49 , CP 115 : coassembly with alpha crystallin but not with vimentin . ^^^ Vimentin , another intermediate filament protein found in the lens , was unable to coassemble with CP 115 , thus demonstrating the specificity of the interaction of CP 49 with CP 115 . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) and western immunoblot analyses showed selective interactions in lens cell homogenates between MBP alphaB and endogenous alphaA and alphaB crystallins , the lens specific intermediate filament proteins phakinin ( CP 49 ) and filensin ( CP 115 ) , and vimentin during a mild 20 minute heat shock at 45 degrees C . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| Using antibodies to filensin and the other lens intermediate filament proteins , vimentin and CP 49 , the codistribution of filensin with CP 49 and independence of this network from the vimentin network was confirmed . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| Vimentin and CP49 / filensin form distinct networks in the lens which are independently modulated during lens fibre cell differentiation . ^^^ The cells of the eye lens contain the type 3 intermediate filament protein vimentin , as well as two other intermediate filament proteins , CP 49 and filensin . ^^^ No correlation was found to changes in the vimentin network but nuclear loss did coincide with changes in the CP49 / filensin network . ^^^ This study demonstrates vimentin and the CP49 / filensin network to be independent in the lens but both networks undergo dramatic changes in subcellular distribution during the differentiation / maturation of the fibre cell . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| Besides the influence of Rb 1 , the lens differentiates under the influence of growth factors ( e . g . , FGF , IGF , PDGF , TGF ) , and specific genes become activated encoding cytoskeletal proteins ( e . g . , filensin , phakinin , vimentin ) , structural proteins ( e . g . , crystallins ) or membrane proteins ( e . g . , Mip ) . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| In the adult lens , filensin and CP 49 are maintained throughout all stages of lens fibre cell differentiation whilst vimentin is apparently lost at a specific stage from the deeper cortical fibres . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| For these purposes , we constructed chimeric proteins in which regions of filensin were exchanged with the equivalent ones of vimentin , a self polymerizing IF protein . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| The filensin gene consists of 8 exons and 7 introns , with 6 introns interrupting its rod domain in a highly conserved manner characteristic of type 3 IF genes , like vimentin , desmin , or peripherin . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| The intermediate filament peptides of the membrane fractions were examined by Western blot analysis , using monoclonal antibodies to filensin , cytoskeletal protein 49 ( CP 49 ) and vimentin . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| Specifically , spectrin ( 235 kDa ) , filensin ( 100 kDa ) , and vimentin ( 57 kDa ) were absent from the SDS PAGE of PSC . ^^^ Increases in filensin and vimentin were observed in a Christmas tree cataract and lamellar cataracts . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| In the present report a model is proposed for the role of tubulin , actin , vimentin , spectrin and the lens specific filaments , filensin and CP 49 , in the establishment and maintenance of transparent lens cell structure . . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| Prenatal and postnatal mice were probed for the IF proteins phakosin , filensin , and vimentin , using light microscope immunocytochemical methodology . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : To describe the intermediate filament proteins vimentin , filensin and phakinin associated with different fractions isolated from neonatal , 10 day old and 20 day old rat lenses . ^^^ Aliquots of the 8 M urea soluble proteins of each fraction were separated by SDS PAGE , transferred to PVDF membranes , the membranes were probed with antibodies to vimentin , filensin or phakinin , and analyzed by computer . ^^^ The concentration of each intermediate filament protein was greatest in the cytoskeletal fraction and over the 20 day period , the amount of vimentin associated with this fraction dramatically decreased , and the amounts of filensin and phakinin dramatically increased . ^^^ Filensin and phakinin associated with the other three major membrane fractions increased over the 20 day growth period , but the level of vimentin did not significantly change . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| METHODS : Indirect immunofluorescence and immunoblot analysis were employed to study the expression of vimentin , beta crystallin , gamma crystallin , filensin , aquaporin 0 and the Na , K ATPase catalytic subunit isoforms ( alpha 1 , alpha 2 , alpha 3 ) in bovine lens epithelium whole mounts as well as lens epithelial cell cultures propagated in medium containing 10 % bovine serum or in medium supplemented with bovine serum concentrations < or =4 % . ^^^ |
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| Interacting proteins: Q12934 and P08670 |
Pubmed |
SVM Score :0.0 |
| This showed the presence of the high molecular weight filamentous structural proteins spectrin , filensin , tubulin , vimentin , actin and phakinin as well as several forms of potential crystallin oligomers comprised of alphaA , betaB 1 , betaA 1 and betaA 4 chains . ^^^ Female rat lenses contained lower levels of aldose reductase , increased proteolyic fragments of the structural proteins filensin , vimentin and phakinin and higher levels of potential alphaA , betaB 1 and betaA 1 crystallin oligomers . ^^^ |
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