Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.83536854
Vimentin and nestin are also expressed in glial progenitor cells and vimentin is expressed along with GFAP in some mature astrocytes . 0.83536854^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
An immunocytochemical investigation has been carried out on five cases of chordoma ( 2 of the sacrum , 2 in the spheno occipital bone and 1 in the parapharyngeal area ) to study the expression of the 5 classes of intermediate filaments ( IF ) : cytokeratin ( CK ) , desmin ( DES ) , GFAP , neurofilaments ( NF ) and vimentin ( VIM ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Quantitative northern blotting was used to examine changes in the levels of mRNAs for the low , middle and high molecular weight neurofilament proteins ( NF L , NF M , NF H ) as well as peripherin , vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Eighteen cases of anaplastic meningioma were studied by LM , EM and immunohistochemistry for vimentin , EMA , keratin , GFAP and S 100 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The present study was performed in order to follow the response of rat cerebellum astroglial cells ( Bergmann glial cells and astrocytes ) to long term portacaval shunt ( PCS ) , by means of glial fibrillary acidic protein ( GFAP ) and vimentin immunoreactivities . ^^^ Since the vimentin content remained unaltered in response to PCS , when compared to control rats , it can be concluded that only the GFAP filaments are affected by PCS . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
By immunogold labelling it was established that GFAP and vimentin were co localized in intermediate filaments of the angiofibroma cells . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
An unusual coexpression of glial fibrillary acid protein ( GFAP ) , keratin and vimentin occurs in pleomorphic adenoma of salivary gland . ^^^ In pleomorphic adenoma , GFAP , cytokeratin and vimentin were coexpressed while in cases of oncocytic adenoma none of the markers was localized . ^^^ Thus , it appears that by using a combination of GFAP , cytokeratin , vimentin and S 100 a distinction between these neoplasms may be possible . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Six chordomas , ten fetal notochords and eight adult notochords were stained for keratin , vimentin , GFAP , desmin , CEA , EMA and s 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The streptavidin biotin immunoperoxidase method was applied to the materials using four monoclonal antibodies : antiglial fibrillary acidic protein ( GFAP ) , anti vimentin ( vimentin ) , LN 1 and LN 3 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Microglial cells may be labeled by staining for LN 1 , ricinus communis agglutinin ( RCA ) 1 , vimentin , HLA DR 2 , and nucleoside diphosphatase , but are negative for Leu M 1 , Leu M 3 , EBM 11 , von Willebrand factor , CD 22 , cytokeratin , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In a pediatric case of necrotizing CMV myelitis after perinatal HIV infection characteristic cytomegalic cells , which could not be attached to a particular cell line by cell morphology , were studied after immunostaining with monoclonal and polyclonal antibodies raised against GFAP , S 100 protein , NSE , synaptophysin , factor 8 , vimentin , macrophages , leukocytes , CMV , HSV 1 + 2 , toxoplasma , and HIV 1 gp 41 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These results indicate that an increase in GFAP and vimentin , the two glial intermediate filament proteins in the area of the ischemic lesion may be associated with a glial response to injury . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical methods were used for GFAP and vimentin , and levels were measured at 4 , 8 , 16 , 30 , 60 , 90 and 120 days . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both cell lines exhibited tumor cell morphology and growth kinetics and showed variable expression of glial fibrillary acidic protein ( GFAP ) , S 100 , fibronectin and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Additionally , the effect of norepinephrine on the phosphorylation of GFAP and vimentin was blocked by alprenolol . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The localization of S 100 protein , glial fibrillary acidic protein ( GFAP ) and vimentin as well as the relations between immunohistochemical reactions , grading and prognoses in 243 gliomas were investigated . ^^^ In astrocytomas , both GFAP and S 100 protein were decreased along with the increase of tumor grades while the vimentin content was coincidently increased . ^^^ The intensity of GFAP were negatively related to that of vimentin . ^^^ Typical neoplastic oligodendroglial cells were known to be devoid of glial filaments and negative to GFAP and vimentin , anyhow , in the sporadic tumor cells , positive reaction to both GFAP and vimentin were identified , and these cells were considered to be either the astrocytes or the transitional cells between astrocytes and oligodendrocytes . ^^^ The results suggested that vimentin and GFAP may exist either independently or coexist synchronously in the astrocytomas as the markers , expressing the anaplasia stages of astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Short clinical course ( preoperative 2 years , postoperative 6 months ) , anaplastic features of glial astrocytic component with scarce GFAP and negative vimentin immunostaining indicate aggressive character of the tumor . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Clones expressing GFAP , A2B5 , and vimentin were regarded as immature astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The development of the ependyma from 6 weeks ( wk ) gestation to term was studied in 26 human fetuses and infants for immunocytochemical differentiation using antibodies against vimentin , several cytokeratins , glial fibrillary acidic protein ( GFAP ) and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Biochemical and tissue distribution studies indicate that MH 1 may recognize four out of five classes of intermediate filament proteins in goldfish : vimentin , desmin , glial fibrillary acidic protein ( GFAP ) , and keratin , but not neurofilament . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibody localization was used to visualize cytokeratin , vimentin , and glial fibrillary acidic protein ( GFAP ) containing intermediate filaments , as well as the adhesive glycoprotein fibronectin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The finding that radial glia and oligodendrocytes expressed similar antigenic features and the additional observation that a small , but consistent fraction of the cells were simultaneously labeled by O 4 and anti GFAP antibodies support the hypothesis that , in the human spinal cord , radial glial cells can give rise to both oligodendrocytes and astrocytes ; in this respect , radial glial cells may be similar to the A2B5+ , GD3+ , vimentin+ bipotential glial progenitors previously identified in cultures from developing rat CNS , which also express A2B5 , GD 3 , and vimentin . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In most cases of tissues with neuroectodermal derivation , coexpression of cytokeratins and vimentin , or triple expression of cytokeratin , vimentin , and GFAP ( fetal and neonatal choroid plexus of humans , rat and guinea pig ependymal cells in the neighborhood of the subcomissural , folliculostellate cells of human and guinea pig pituitary ) is detectable . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Thirty five paraffin embedded medulloblastomas ( 19 from children and 16 from adults ; 24 classic medulloblastomas , 10 desmoplastic medulloblastomas , 1 tumor with neuronal differentiation ) were examined for reactions with antibodies against glial fibrillary acidic protein ( GFAP ) , cytokeratins KL 1 and MNF 116 , desmin , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immuno electron microscopy was used to localize the distribution of vimentin and glial fibrillary acidic protein ( GFAP ) in mouse astrocytes and their precursor cells in primary cultures . ^^^ In astroblasts and astrocytes , vimentin and GFAP form intermediate filaments ( IF ) , which are heteropolymers , as previously observed in gliomas . ^^^ Astrocytes and their precursor cells may have IF composed of GFAP vimentin heteropolymer or vimentin alone , but IF composed of GFAP only were not seen . ^^^ It seems that the formation of IF that are GFAP vimentin heteropolymers is a feature of normal astroglia development and that the ratio of GFAP to vimentin in these IF reflects the degree of differentiation and functional state of the cell . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The histogenesis of the hamster dentate gyrus was studied with light and electron microscopy and antisera against the astrocyte associated antigens vimentin and GFAP , in order to follow the differentiation of radial glial cells and astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present work , coexpression of vimentin ( VIM ) and glial fibrillary acidic protein ( GFAP ) is demonstrated in the glial cells of the adult rat pineal gland . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Remarkable vimentin expression in SAs , instead of GFAP , strongly suggests that SAs are not going to degenerate but are bearing up to regenerate in such circumstances . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Morphological examination using light and electron microscopy revealed profound neuronal changes associated with a marked increase in glial fibrillary acidic protein ( GFAP ) and in vimentin immunoreactivities , together with a substantial reduction in myelin basic protein ( MBP ) immunostaining in the brain . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GISTs were immunopositive in the following categories : vimentin 45 of 46 , desmin nine of 45 , muscle specific actin ( MSA ) 36 of 46 , alpha smooth muscle actin ( SMA ) 34 of 46 , chicken gizzard actin 7 zero of 38 , cytokeratin two of 46 , S 100 protein six of 46 , glial fibrillary acidic protein ( GFAP ) zero of 46 , synaptophysin zero of 46 , and chromogranin one of 46 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Myoepithelial cells were often immunopositive for S 100 protein , vimentin , glial fibrillary acidic protein ( GFAP ) , and alpha smooth muscle actin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Gliomas induced in the rat by transplacental administration of ethylnitrosourea ( ENU ) are intensely immunoreactive for vimentin and scarcely for glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Thereafter , the distribution of autographically labelled astrocytes expressing glial fibrillary protein ( GFAP ) and astrocyte like cells expressing vimentin were recorded within the region of injury . ^^^ However , on day 4 the number of proliferating GFAP positive astrocytes was about 50 % higher than that of vimentin positive cells . ^^^ Those facts were considered as an evidence against the hypothesis that a reactive astrocyte division induces a two stage increase in the cytoskeletal proteins level with the elevated synthesis of vimentin preceding that of GFAP . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumours were tested for reactivity with monoclonal antibodies against vimentin and desmin and with polyclonal antibodies against cytokeratin , S 100 protein , glial fibrillary acidic protein ( GFAP ) and neuron specific enolase ( NSE ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry was employed in these lesions using antibodies against neurofilament protein ( NFP ) , glial fibrillary acidic protein ( GFAP ) , vimentin ( VM ) and myelin basic protein ( MBP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present work the expression of vimentin and glial fibrillary acidic protein ( GFAP ) was determined in homotypic , transitional and heterotypic astroglial neoplastic areas and gemistocytes . ^^^ The intensity of vimentin and GFAP immunoreactivity as well as the number of immunoreactive cells within astroglial areas of different grades of differentiation were different . ^^^ Within homotypic astroglial areas the number of GFAP positive cells and intensity of GFAP immunoreactivity were higher than the same vimentin parameters . ^^^ It is well known that vimentin and GFAP may form heteropolymers both in vitro and in vivo . ^^^ Transitions in vimentin / GFAP expression reflect not only normal development of astroglial cells but occur also with the induction of neoplastic process . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies against neuron specific enolase ( NSE ) , S 100 protein , neurofilaments protein ( NFP ) , glial fibrillar acidic protein ( GFAP ) , myelin basic protein ( MBP ) , Synaptophysin ( SYN ) and vimentin were used . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of NP 185 was compared with those of other cytoskeletal and cytoplasmic proteins of axons and synapses , namely synaptophysin , vimentin , neurofilament NF 68 , and the intermediate filaments of glial cells ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Mller ( glial ) cells were well developed and could be immuno labelled by antibodies against both vimentin and glial fibrillary protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of the cytoskeletal elements cytokeratin 18 , vimentin , desmin , glial fibrillary acid protein ( GFAP ) , and actin was investigated in different portions of the ciliary body of human and bovine eyes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to determine whether this is the case , we combined adrenalectomy , with or without corticosterone ( CORT ) replacement , and 3H thymidine autoradiography , Nissl staining , and immunohistochemistry for the glial cell markers vimentin and glial fibrillary acidic protein ( GFAP ) as well as for the neuronal marker neuron specific enolase . ^^^ Removal of circulating adrenal steroids resulted in a greater density of both GFAP immunoreactive and vimentin immunoreactive cells compared to sham operated animals ; CORT replacement prevented increases in both of these cell types . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Sexual dimorphism in the hamster cerebellum demonstrated by glial fibrillary acidic protein ( GFAP ) and vimentin immunoreactivity . ^^^ Male and female hamsters aged 1 , 4 , and 10 postnatal weeks were used to study the distribution of vimentin and glial fibrillary acidic protein ( GFAP ) in the cerebellum . ^^^ Vimentin immunoreactivity exceeded that of GFAP during the first postnatal week , although GFAP was also observed in all cerebellar layers . ^^^ At 10 weeks , vimentin immunoreactivity was reduced to thin Bergmann glial processes , whereas GFAP immunoreactivity had greatly increased in the whole cerebellum . ^^^ The GFAP immunostaining was denser in males than in females ; however , in females , the Bergmann fibers were heavily immunostained with anti vimentin in contrast to the males . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In reaggregating cultures GFAP , vimentin and actin synthesis was approximately 52 % , 50 % and 37 % the level found in monolayer cultures , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
It included antibodies against cytokeratin ( CK ) , vimentin ( VIM ) , glial fibrillary acidic protein ( GFAP ) , and muscle specific actin ( MSA ) ; the latter three markers are associated with myoepithelial differentiation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
On the basis of immunofluorescence microscopy using multiple double labeling with antibodies to glial fibrillary acidic protein ( GFAP ) , vimentin , and the recently identified intermediate filament associated protein ( IFAP ) 70 / 280kD , four distinct astrocytic subtypes were definable in neonatal rat brain astrocytes in culture . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The majority of accumulation phase blastemal cells labeled with antibodies against GFAP , vimentin , 22 / 18 as well as with antibodies against NCAM , L 1 , laminin , and fibronectin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Gliafibrillar acid protein ( GFAP ) and vimentin proved to be of particularly high stability and , consequently , were easily detectable from paraffin material . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of intermediate filaments GFAP , Vimentin and Desmin was investigated in normal intracerebral and meningeal vessels in human material . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The gustatory centers ( vagal lobes and facial lobe ) and the tegment of the posterior rhombencephalon of the Iberian barb ( Barbus comiza ) have been studied using anti glial fibrillary acidic protein ( anti GFAP ) and anti vimentin immunohistochemical techniques . ^^^ GFAP immunoreactivity was found in the tegment and in a part of the vagal lobes while vimentin immunoreactivity was located in the tegment . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These cases were further studied with the anti epithelial non cytokeratin antibodies EMA , HEA 125 , Ber EP 4 , CEA as well as the melanoma antibody HMB 45 , Leu M 1 , GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At time 0 , the percentages of cells decorated by monoclonal antibodies specific for bromodeoxyuridine ( BrDU ) after a 2 . 5 h pulse , and for glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) were respectively 8 + / 2 , 15 + / 4 and 10 + / 3 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have studied the expression of the intermediate filament ( IF ) proteins , vimentin and glial fibrillary acidic protein ( GFAP ) , in cultured human Schwann cells ( SC ) from patients with different neuropathies and normal control cases . ^^^ SC cultures from sural nerve biopsies of 8 subjects with axonal neuropathies , 8 with demyelinating neuropathies and 3 normal controls were included in this study and processed with double immunofluorescence technique , using anti vimentin and anti GFAP antibodies , during the 2nd , 4th and 6th week of culture . ^^^ We have found that : ( 1 ) cultured human SC constantly express vimentin ; ( 2 ) SC from normal controls are GFAP negative in the first period of culture ; ( 3 ) SC from pathologic nerves can contain GFAP immunoreactive IF and the percentage of GFAP positive SC is higher in axonal than in demyelinating neuropathies ; ( 4 ) during the permanence in culture human SC from both normal and pathologic cases acquire the ability to synthesize GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Globoside colocalized with vimentin in human and mouse fibroblasts , with desmin in smooth muscle cells , with keratin in keratinocytes and hepatoma cells , and with glial fibrillary acidic protein ( GFAP ) in glial cells . ^^^ These observations indicate that globoside colocalizes with vimentin , desmin , kertain and GFAP , with a preference for vimentin in cells that contain both vimentin and keratin networks . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Serial sections of the conus medullaris and the filum terminale of 23 randomly selected human spinal cords were studied by light and electron microscopy , and following immunoperoxidase staining for glial fibrillary acidic protein ( GFAP ) , vimentin , neuron specific enolase ( NSE ) , amyloid beta protein , and S 100 protein . ^^^ A minority of the glial cells showed GFAP immunoreactivity , and some were immunoreactive for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Seventeen intracerebral gliomas containing Rosenthal fibers ( RF ) were studied by an immunoperoxidase method for localization of ubiquitin ( UB ) , glial fibrillary acidic protein ( GFAP ) , desmin and vimentin ( VIM ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
It has been postulated that coexpression of GFAP and vimentin in neoplastic myoepithelial cells in pleomorphic adenomas reflects early chondroid differentiation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
It is known that these cells co express the intermediate filaments cytokeratin , vimentin , and occasionally GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cultured cells of explants from 23 human gliomas ( seven astrocytomas , eleven anaplastic astrocytomas , three ependymal tumors and two medulloblastomas ) were studied to examine cell morphology and expression of glial fibrillary acidic protein ( GFAP ) , vimentin , fibronectin , and N myc oncoprotein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Electron immunocytochemical staining for three intermediate filament ( IF ) proteins , keratin ( K ) , glial fibrillary acidic protein ( GFAP ) , and vimentin ( 5 ) , and for the macrophage marker , EBM / 11 ( E ) , was performed on epiretinal membranes obtained during vitrectomies performed for proliferative vitreoretinopathy ( PVR ) , postdetachment macular puckers ( PDMPs ) , idiopathic macular puckers ( IMPs ) , or macular puckers associated with other disease processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Fifty one non neoplastic human pituitary glands , including examples with Crooke ' s hyalinization or amyloidosis , were examined by an immunoperoxidase method using antibodies to keratin , vimentin , neurofilaments ( NFs ) , glial fibrillary acidic protein ( GFAP ) , desmin , actin , S 100 protein and a variety of pituitary hormones . ^^^ This study also demonstrated that keratin and NFs may be co expressed in endocrine cells in the pituitary anterior lobe , that keratin , vimentin and GFAP may be co expressed in the epithelial cells forming cyst like follicle in the pars intermedia , and that vimentin and GFAP may be co expressed in folliculo stellate cells and pituicytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial cell processes were visualized immunohistochemically with antibodies to glial fibrillary acidic protein ( GFAP ) or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Retinal pigment epithelial ( RPE ) cells , retinal glial , and fibroblasts , three cell types believed to play a role in the pathogenesis of epiretinal membrane formation , were maintained in vitreous culture to determine the influence of vitreous on their ultrastructure and expression of cytokeratin , glial fibrillary acidic protein ( GFAP ) , vimentin , and glutamine synthetase ( GS ) . ^^^ Retinal glial grown on vitreous showed a time dependent decrease in the number of cells expressing GFAP and a corresponding increase in cells expressing vimentin or GS . ^^^ A substantial number of cells in each culture did not stain positively for cytokeratin , GFAP , vimentin , or GS . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ultrastructural immunoreactivities of alpha B crystallin , glial fibrillary acidic protein ( GFAP ) , ubiquitin , and vimentin in Rosenthal fibers ( RFs ) isolated from an Alexander ' s disease brain were investigated using nonosmium and low temperature embedding technique . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Routine immunohistochemical characterization of short term in vitro explants from human intracranial tumours . 35 intracranial tumours , 18 gliomas , 12 meningiomas , one neurilemmoma ( neurinoma ) , one malignant melanoma and two metastases were successfully grown in vitro and were submitted to immunocytochemical reactions , including cytokeratin , glial fibrillary acid protein ( GFAP ) , vimentin , fibronectin , S 100 protein , neurofilament proteins , neuron specific enolase ( NSE ) and basic myelin protein ( MBP ) . ^^^ Cytokeratin in metastases , GFAP and vimentin in gliomas , vimentin in meningiomas were consistently positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The GFAP immunoreactive cells also express a panel of myoepithelial cell markers , including cytokeratin 14 ( CK 14 ) , vimentin , smooth muscle specific actin isoforms , nerve growth factor receptor ( NGFR ) and common acute lymphoblastic leukaemia antigen ( CALLA ) . ^^^ Furthermore , in all the instances of fibroadenoma , phyllodes tumour , epitheliosis and gynaecomastia , a variable number of epithelial cells also acquires immunoreactivity for GFAP , vimentin , CK 14 , NGFR and , to a lesser extent , for CALLA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial fibrillary acidic protein ( GFAP ) , vimentin , desmin , neurofilaments , and broad specificity keratin MAbs , as well as MAbs that recognize several or only single keratin polypeptides , were used . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Primary antisera included glial fibrillary acidic protein ( GFAP ) , HNK 1 ( type 1 astrocyte precursor marker ) , A2B5 ( type 2 astrocyte precursor marker ) , S 100 , vimentin , myelin basic protein ( MBP ) , laminin , keratin , cytokeratin , epithelial membrane antigen ( EMA ) , and neuron specific enolase ( NSE ) . ^^^ Neoplastic astrocytes in optic nerve gliomas stained with GFAP , HNK 1 , S 100 , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At the interface between the meninges and the central nervous system there is a characteristic structure known as the glia limitans , consisting of many fine interdigitating astrocyte processes which contain both GFAP and vimentin , and a basal lamina . ^^^ Cultured meningeal cells were injected into the hippocampus of adult rats , and from 1 to 12 weeks later brains were stained were stained for GFAP and vimentin . ^^^ One week after injection there was a widespread astrocytic reaction stretching up to 2 mm from the injection , the cells being stained intensely for both GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have studied paraffin embedded specimens of 17 rat granular cell brain tumors ( GCBT ) from four long term drug safety carcinogenicity studies by peroxidase antiperoxidase ( PAP ) immunohistochemistry with either polyvalent or monoclonal antibodies against glial fibrillary acidic protein ( GFAP ) , S 100 protein ( S 100 ) , Leu 7 epitopes , vimentin ( VIM ) , keratin , desmin , and myelin basic protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of the following antigens was also studied : epithelial membrane antigen ( EMA ) , human natural killer ( HNK 1 ) , glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Increased vimentin expression associated with a decrease of GFAP immunoreactivity correlated with anaplasia and short survival . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of glial fibrillary acidic protein ( GFAP ) , vimentin , S 100 protein ( S 100 ) , HNK 1 , myelin basic protein ( MBP ) and fibronectin was investigated immunohistochemically in 51 ethylnitrosourea ( ENU ) induced neurinomas of the rat . ^^^ In the RN 6 neurinoma clone , vimentin and GFAP could be demonstrated both in vivo and in vitro . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Co expression of immunoreactivity for GFAP and vimentin was observed in adrenocortical and Leydig cells of the Syrian hamster but there were differences in the staining patterns of these intermediate filament proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
An immunohistochemical study of the production of the intermediate filaments [ vimentin , cytokeratin , and glial filament acidic protein ( GFAP ) ] during development of the pituitary gland was made by use of fetal and adult human pituitary tissue . ^^^ Among these intermediate filament proteins in the anterior and intermediate lobes of the pituitary , cytokeratin is the first to appear , followed by GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The sensory nerve formations ( or corpuscles ) of normal human glabrous skin from hand and fingers , obtained by punch biopsies , were studied by the streptavidin biotin method using monoclonal antibodies directed against neurofilament protein ( NFP ) , S 100 protein , glial fibrillary acidic protein ( GFAP ) , cytokeratins , and vimentin . ^^^ The present results suggest that the main nature of the intermediate filaments of the non neuronal cells of sensory nerve formations from human glabrous skin is represented by vimentin and not by GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These studies describe a cytoskeletal associated protein kinase activity in astrocytes that phosphorylated the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin and that appeared to be distinct from protein kinase C ( PK C ) and the cyclic AMP dependent protein kinase ( PK A ) . ^^^ In addition , it was determined that the intermediate filament protein kinase activity phosphorylated both serine and threonine residues of the intermediate filament proteins , vimentin and GFAP . ^^^ Also , the intermediate filament protein kinase activity phosphorylated both the N and the C terminal domains of vimentin and the N terminal domain of GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both GFAP and vimentin , another intermediate filament protein , marked the specialized ependymal cells of this region , the tanycytes . ^^^ At the light microscopic level , double label immunocytochemical studies did not reveal any specific association between GFAP or vimentin positive cellular processes and these pioneer GnRH fibers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The present paper describes the analysis of immunostaining patterns of four antibodies , alpha 1 antitrypsin ( alpha AT ) , glial fibrillary acid protein ( GFAP ) , S 100 protein ( S 100 ) , and vimentin in 31 human gliomas . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A gradual transition from vimentin to glial fibrillary acidic protein ( GFAP ) reactivity in the radial glial cells occurs at week 8 . ^^^ The S 100 positive astrocytes are thought to be derived from ventricular zone cells , which at the time of their appearance do not express any of the applied astrocytic markers ( S 100 , GFAP , vimentin ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Under normal conditions , the ependymal cells are vimentin positive and negative for GFAP and the IF , respectively , while GFAP and NF positivity is found in the grey and white matter . ^^^ During regeneration , by about day 10 , vimentin positivity begins to decrease in the ependymal epithelium of the caudal stump and GFAP positive elements appear ( subsequently , this was observed also in the ependymal epithelium of the cephalic stump ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This study was performed to identify cellular components of the membranes by means of immunohistochemical technique . 11 proliferative vitreoretinal membranes which were surgically obtained from 7 eyes with PVR and 4 eyes with PDR were stained with monoclonal antibodies against cytokeratin , glial fibrillary acidic protein ( GFAP ) , or vimentin using immunoperoxidase technique ( ABC method ) . ^^^ In the PVR membranes , mean cell positivities for cytokeratin , GFAP and vimentin were 48 % , 1 % and 92 % , respectively and in the PDR membranes , 0 % , 5 % and 93 % , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunohistochemical expression of glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) was studied in reactive astrocytes of the rat cerebral cortex 5 days after a brain injury . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies to vimentin and glial fibrillary acidic protein ( GFAP ) were used . ^^^ During the first postnatal weeks , vimentin was replaced by GFAP which is therefore expressed in a similar radial pattern . ^^^ This orderly structural organisation of vimentin and GFAP in the matrix layer could indicate the involvement of both proteins in morphogenetic processes such as neuron migration and cell organisation . ^^^ In the mantle layer , a distinct radial vimentin immunoreactivity was replaced by GFAP immunoreactivity during the first 2 postnatal weeks . ^^^ In the ventral and dorsal raphe , a major vimentin expression was replaced by a minor presence of GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Broad range monoclonal antibodies ( mAB ) against cytokeratins ( CK ) ( lu 5 , A 45 B / B3 , AE1 / 3 , CAM 5 . 2 ) as well as anti CK 18 ( DC 10 ) and anti CK 19 ( A 53 B / A2 ) were compared with mAB ' s against vimentin , epithelial membrane antigen ( EMA ) , epithelial sialomucin ( ESM 140 C 1 ) , GFAP ( GF 2 ) , neurofilament ( 2F11 ) , Leu 7 ( HNK 1 ) and polyclonal AB ' s against pituitary hormones ( ACTH , FSH , LH , TSH , GH , PRL ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The reduction in GFAP concentration together with the delayed vimentin GFAP transition could explain how astrocyte morphogenesis is impaired by neonatal thyroid deficiency . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Virtually all passaged cells ( greater than 98 % ) contain vimentin positive intermediate filaments and no glial fibrillary acidic protein ( GFAP ) has been detected using a range of GFAP antibodies known to label astrocytes in the guinea pig optic nerve . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Double label immunofluorescence revealed many vimentin positive cells and processes that were also labeled with GFAP antibody . ^^^ In addition , cells and processes that were vimentin negative but GFAP positive were also found in the degenerating tract . ^^^ We suggest that the reactive cells which possessed both vimentin and GFAP were reactive astrocytes of astroblastic origin while those that expressed only GFAP were derived from mature astrocytes . ^^^ Other vimentin positive cells / processes did not label with anti GFAP and thus were either microglial , endothelial or inflammatory cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have now extended these studies to assess the effect of TNF on message levels for the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ The results have shown that , whereas TNF had only a slight effect on vimentin mRNA , TNF induced a marked decrease to 4 . 3 + / 2 . 0 % of controls in GFAP mRNA which was both time and dose dependent . ^^^ Polyacrylamide gel electrophoretic analysis of astrocyte cytoskeletal proteins demonstrated that GFAP levels were reduced after a 5 day incubation with 200 U / ml TNF whereas protein levels of vimentin and actin were not significantly changed . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In fibroblasts with a preexisting vimentin network , injected porcine glial fibrillary acidic protein ( GFAP ) co localized with the vimentin network within 24 hours . ^^^ After microinjection of either porcine GFAP or murine vimentin into human carcinoma cells lacking cytoplasmic intermediate filaments , we observed that different types of filament networks developed . ^^^ Whereas vimentin was incorporated into short filaments immediately after injection , GFAP was found to aggregate into rodlike structures . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor cells did not show any positive reaction with antibodies to GFAP , S 100 protein , NSE , vimentin , cytokeratin , desmin , and von Willebrand factor ( factor 8 related antigen ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
PMA also increased 32P incorporation into several proteins , including glial fibrillary acidic protein ( GFAP ) , vimentin , and proteins with molecular weights of 80 , 000 ( pI 4 . 5 ) , 50 , 000 ( pI 4 . 9 ) , and 30 , 000 ( pI 5 . 5 ) . ^^^ Analysis of phosphorylation sites on GFAP and vimentin using two dimensional tryptic mapping techniques indicate that the partial inhibition of phosphorylation is likely the consequence of partial inhibition of protein kinase C rather than a selective inhibition at some phosphorylation sites and not others . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
S 100 was focally positive in three of the colloid and one of the enterogenous cysts while vimentin and GFAP were negative in both . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The alpha and beta subunits of S 100 protein , vimentin , and glial fibrillary acidic protein ( GFAP ) , which are reliable markers of astrocytic cells , were demonstrated in a large number of cells by immunoperoxidase staining . ^^^ The results of immunoblotting showed that the expression of vimentin was much higher than that of GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical study on this tumor showed negative NF and GFAP ; positive NSE , S 100 , vimentin and keratin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have studied 27 oligodendrogliomas with a panel of antibodies ( vimentin , GFAP , S 100 protein , myelin basic protein , CAM 5 . 2 ) and of lectins ( WGA , Con A , PNA , RCA , DBA , SBA ) to different glycoproteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial fibrillary acidic protein ( GFAP ) and vimentin were analyzed as intermediate filament ( IF ) proteins . ^^^ Fluorescence studies revealed some differences between the IF distribution patterns of GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemical staining for other putative astrocytic antigens demonstrated glutathione S transferase ( Yb isoenzyme ) to be localized extensively in GFAP positive cells and vimentin to be present both in inflammatory cells and in some GFAP positive astroglial cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The glial cells immunolabeled for glial fibrillary acidic protein ( GFAP ) , carbonic anhydrase C ( CA C ) , vimentin , and glutamine synthetase ( GS ) , thus suggesting that they were Mller ' s cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
No changes in total cellular levels of GFAP , vimentin , tubulin or actin were observed over a 2 h period of treatment . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To investigate the function of GFAP , we have studied the human astrocytoma cell line , U 251 , which constitutively expresses GFAP and vimentin in the same 10 nm filaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Na ( + ) current expression was studied in hippocampal astrocytes in vitro , individually identified by filling with Lucifer yellow ( LY ) and staining for glial fibrillary acidic protein ( GFAP ) and vimentin . 2 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both the protein kinase C ( PK C ) activator , phorbol 12 myristate 13 acetate ( PMA ) , and the cyclic AMP dependent protein kinase ( PK A ) activator , 8 bromo cyclic AMP ( 8 BR ) , have been shown to increase 32P incorporation into glial fibrillary acidic protein ( GFAP ) and vimentin in cultured astrocytes . ^^^ Therefore , studies were conducted to determine whether treatment with PMA and 8 BR results in the phosphorylation of the same tryptic peptide fragments on GFAP and vimentin in astrocytes . ^^^ Treatment with PMA increased 32P incorporation into all the peptide fragments that were phosphorylated by 8 BR on both vimentin and GFAP ; however , PMA also stimulated phosphorylation of additional fragments of both proteins . ^^^ The phosphorylation of vimentin and GFAP resulting from PMA or 8 BR treatment was restricted to serine residues in the N terminal domain of these proteins . ^^^ Studies were also conducted to compare the two dimensional tryptic phosphopeptide maps of GFAP and vimentin from intact cells treated with PMA and 8 BR with those produced when the proteins were phosphorylated with purified PK C or PK A . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The cells are negative for GFAP , positive for vimentin , express Ia antigen and take up DiL Ac LDL . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A surgically resected medulloblastoma of the left cerebellum in a 42 year old man contained numerous mature fat cells ; many of these adipocytes expressed glial fibrillary acidic protein ( GFAP ) , S 100 protein , and vimentin as seen by immunocytochemistry . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry technique revealed that the tumor cells were negative for GFAP , positive for Vimentin , positive for S 100 protein and negative for EMA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the primary tumor , GFAP , vimentin , S 100 , Leu 7 and glioma associated antigens ( GAA ) as defined by the monoclonal antibodies ( mAbs ) MUC 2 39 , MUC 8 22 and MUC 2 63 were markedly expressed . ^^^ In the recurrences , gradual loss of GFAP and Leu 7 could be observed , whereas S 100 , vimentin and GAA gave similar results to those in the primary tumor . ^^^ In long term passages , immunoreactivity of GFAP , Leu 7 and S 100 decreased , whereas GAA , vimentin and fibronectin increased . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
There was no relation between GFAP or vimentin expression and histology or GSL type both with regard to final and regional diagnoses . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP , NSE and vimentin were found in large cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunohistochemical study shows the value of GFAP , S 100 protein , HNK 1 and Vimentin as specific markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The presence of some cytoskeletal proteins related to the intermediate filaments glial fibrillary acidic protein GFAP and vimentin ) and S 100 protein has been investigated in sensory formations of the glabrous skin of the rat . ^^^ A positive reaction both for S 100 protein and vimentin was found in the inner core and related cells of glomerular and simple sensory corpuscles ; in contrast , no positive reaction was shown for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The occurrence of vimentin positive and GFAP negative cells in gliomas induced by transplacental ENU in the rat is not yet clarified . ^^^ Because of the temporal overlapping between neural cytogenesis and tumoral transformation in this experimental model , the expression of vimentin and GFAP in the developing rat brain can give some information . ^^^ Vimentin is constantly present in the processes of radial glia ; the earliest GFAP positive cells are mature astrocytes appearing when vimentin positive radial glia is still abundantly present . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of neuron specific enolase ( NSE ) , Leu 7 ( LEU ) , S 100 protein ( S 100 ) , glial fibrillary acidic protein ( GFAP ) , neurofilament protein ( NF ) , vimentin ( VIM ) , cytokeratin ( CKER ) , and desmin ( DES ) was also assessed with antisera and MAbs . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Normal pituitaries were stained for each of the five known IF ( Cytokeratins ( CK ) , glial fibrillary acidic protein ( GFAP ) , desmin , vimentin , and neurofilaments ( NF ] . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Second , different cell markers can be expressed by one and the same cell ( e . g . , GFAP , NSE , vimentin ) , making nosological interpretation of the tumor difficult , impossible , or at best rather subjective . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have investigated the actions of Ca 2 ( + ) calmodulin ( CaM ) dependent protein kinase 2 on various types of non epithelial intermediate filament proteins , vimentin , desmin , glial fibrillary acidic protein ( GFAP ) and neurofilament triplet proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
No p 38 immunoreactivity was discernible in the interstitial glial cells , which showed a heterogeneous pattern of immunostaining for the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
All 35 tumors of the nervous system were treated by a panel of immunohistochemical reactions comprising antibodies against cytoskeleton intermediary filaments such as GFAP ( glial fibrillary acid protein ) , neurofilament proteins , vimentin and cytokeratins and some nervous system antigens such as NSE ( neuron specific enolase ) , MBP ( myelin basic protein ) and S 100 protein . ^^^ In central tumors , considered to be malignant gliomas , focal reactivity against vimentin and GFAP was found . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have studied the ultrastructural distribution of GFAP , vimentin , FVIII / RAg and collagen 4 in biopsies of human gliomas with various degree of anaplasia . ^^^ In astrocytes , GFAP and VIM were localized in the same filament bundles ; GFAP was always more expressed than vimentin , regardless of the malignancy grade . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin and glial fibrillary acidic protein ( GFAP ) were quantified by counting of immunolabeled cells and immunoblotting . ^^^ Vimentin and GFAP content rose from DIV 3 to 6 in all cultures . ^^^ Regional variation of vimentin content was low , but large differences occurred in amounts of GFAP . ^^^ Simultaneous immunostaining for both cytoskeletal proteins revealed the presence of large numbers of vimentin single labeled and small numbers of vimentin / GFAP double labeled cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial fibrillary acidic protein ( GFAP ) and vimentin proteins are known to be component proteins of glial filaments in the CNS of many vertebrates . ^^^ The nature of the filaments present in the glial cells of the goldfish optic tectum and in the CNS of two members of the Mollusca ( Helix pomatia and Octopus vulgaris ) were investigated using immunocytochemical localization of monoclonal antibodies to GFAP and vimentin . ^^^ Immunoblots visualized by the alkaline phosphatase method showed cross reactive protein bands to GFAP and vimentin antibodies in total brain homogenates of the goldfish , octopus , and snail . ^^^ Unlike anti GFAP antibodies , which stained relatively specific to filaments , vimentin staining in the CNS tissues of the three organisms studied did not appear to be exclusive to filamentous structures . ^^^ The proteins GFAP and vimentin which are thought to be well conserved in vertebrate evolution also appear to be expressed in the nervous system of some lower organisms . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The majority of cells in the cultures expressed glial fibrillary acidic protein ( GFAP ) , frequently in conjunction with vimentin and fibronectin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to determine whether or not thyroid hormone influences the development of astrocytes in brain regions where neurons are affected , we performed vimentin and glial fibrillary acidic protein ( GFAP ) immunocytochemical and single section Golgi impregnation analyses on the basal forebrain and hippocampus of control and neonatally thyroid hormone treated rats . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To study the relationship of Mller cell changes to the vascular alterations in the dystrophic retina , we used immunoperoxidase techniques and antibodies against GFAP and vimentin . ^^^ Studies of vimentin distribution in the dystrophic retina showed results similar to the GFAP study . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Perithelial spindle cell proliferations and intramural spindle cells within thick walled vessels stained for GFAP , S 100 protein and / or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Dibutyryl cyclic AMP induces vimentin and GFAP expression in cultured medulloblastoma cells . ^^^ Astrocyte progenitor cells from the mouse neopallium however express another IF protein , vimentin , before they acquire GFAP in vivo and in vitro . ^^^ The purpose of the current study was to determine if cells obtained from a focally GFAP positive posterior fossa medulloblastoma previously shown to acquire GFAP in response to dibutyryl cyclic AMP ( dBcAMP ) , also express vimentin before expressing GFAP . ^^^ More than 80 % of cells in the tumor section contained vimentin while fewer than 1 % of cells contained GFAP ; occasional clusters of malignant GFAP positive cells and clusters of cells negative for both vimentin and GFAP were also identified in the tumor . ^^^ One hundred per cent of cultured cells in the first 10 passages from the tumor contained vimentin and no cells expressed GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The histological features of the initial tumour were conserved in the hetero transplanted tumours , which consisted of vimentin and GFAP immunoreactive astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The removed tumor , which was examined immunohistochemically , was positive for vimentin , GFAP , NF 68 , SYP , and S 100 ; and negative for CEA , desmin , and cytokeratin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Given the previously described coexpression of vimentin and GFAP by neoplastic cartilage , it appears likely that this immunophenotype in neoplastic myoepithelial cells reflects early chondroid differentiation . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP immunoreactive chondrocytes also showed apparent immunostaining for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In addition , the results show that astrocyte expression of vimentin is more specific than GFAP in identifying regions of permanent ischemic injury during the early postischemic period . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We investigated the expression of vimentin and glial fibrillary acidic protein ( GFAP ) within Mller cells in normal human retinas and in detached human retinas of proliferative vitreoretinopathy ( PVR ) cases using the immunogold method . ^^^ Mller cells in normal retinas showed vimentin immunoreactivity and faint GFAP immunoreactivity ; however , in detached retinas they showed distinct GFAP immunoreactivity as well as vimentin immunoreactivity . ^^^ Immunoelectron microscopic observation revealed that intermediate filaments ( IF ) within Mller cells in normal retinas showed vimentin immunoreactivity and that those within Mller cells in detached retinas showed both vimentin and GFAP immunoreactivity . ^^^ Double staining for vimentin and GFAP showed that in detached retinas , these two protein immunoreactivities were observed in the same filaments . ^^^ These results indicate that IF of human Mller cells consist of vimentin under normal conditions and that Mller cells in detached retinas contain different IF , which consist of vimentin and GFAP . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemical techniques were used to look for the existence of two classes of intermediate filaments , vimentin and glial fibrillary acidic protein ( GFAP ) , in cellular elements of the inner ear epithelium in normal and experimentally damaged guinea pig cochleas . ^^^ Cochlear hair cells were not labeled for either GFAP or vimentin . ^^^ Following hair cell destruction by exposure to noise or the administration of aminoglycosides , GFAP and vimentin are not present in phalangeal scars replacing lost hair cells . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression of glial fibrillary acidic protein and vimentin in mouse lens epithelial cells during development in vivo and during proliferation and differentiation in vitro : comparison with the developmental appearance of GFAP in the mouse central nervous system . ^^^ Analysis of glial fibrillary acidic protein ( GFAP ) and vimentin in mouse lens epithelial cells ( MLEC ) during ontogenesis revealed a two step developmental expression similar to that observed in astrocytes . ^^^ Vimentin was first immunostained at E 11 corresponding with the closure of the lens vesicle , whereas GFAP was detected only after a further 7 days ( E 18 ) ; this protein appeared simultaneously in the mouse lens and CNS . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The localization of vimentin ( VIM ) and glial fibrillary acidic protein ( GFAP ) was studied immunohistochemically in adult rat optic nerve . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The antibodies anti S 100 , anti GFAP and anti vimentin have supported the presence of glial and mesenchymal elements . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunostaining patterns of glial fibrillary acidic protein ( GFAP ) , S 100 protein ( S 100p ) and vimentin were studied using immunohistochemical techniques on 48 paraffin embedded glial tumors . ^^^ In many astrocytomas with different degrees of malignancy co expression of GFAP , S 100p and vimentin was found . ^^^ It seems that GFAP is a superior marker to S 100p and vimentin in the identification of human gliomas . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Exposure to PMA also produces increases in the phosphorylation of several proteins including vimentin , glial fibrillary acidic protein ( GFAP ) , an acidic 80 , 000 molecular weight protein , and two 30 , 000 molecular weight proteins ( pI 5 . 5 and 5 . 7 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We examined the effect of lactic acid on cultured human glioma cell lines expressing glial fibrillary acidic protein ( GFAP ) , vimentin and neuron specific enolase ( NSE ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We analyzed expression of the following markers by immunocytochemistry : glioma specific antigens ( GE 2 and CG 12 ) , fibronectin , intermediate filaments ( GFAP , vimentin , neurofilaments ) , class 1 and 2 histocompatibility antigens ( HLA ABC and HLA DR ) , growth factor and receptor ( alpha TGF and EGF receptor ) , proliferation associated antigen ( Ki 67 ) . ^^^ Strong and stable staining with the two antiglioma monoclonal antibodies ( GE 2 and CG 12 ) was seen , with coexpression of GFAP and fibronectin in five of six cell lines ( after 20 passages ) and presence of vimentin and neurofilaments . ^^^ A slight decrease in the positivity of some markers ( GFAP , vimentin and HLA DR in 2 / 6 cell lines ) was observed after prolonged in vitro culture ( greater than 12 months ) , but morphophenotypic modifications , established within a few passages after explanation , were maintained with time . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Radial glia and astrocytes in developing and adult telencephalon of the lizard Gallotia galloti as revealed by immunohistochemistry with anti GFAP and anti vimentin antibodies . ^^^ The development of radial glia and astrocytes in the telencephalon of the lizard Gallotia galloti was studied by immunohistochemistry with anti vimentin and anti GFAP antibodies . ^^^ Radial glia is still present in the adult , and the composition of its intermediate filaments changes during development from vimentin to GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of glial fibrillary acidic protein ( GFAP ) and vimentin containing cells was studied by immunohistochemistry in the midbrain of the lizard Gallotia galloti . ^^^ Faint GFAP immunoreactivity gradually appeared in the same structures between E 34 and E 37 , and this increased until adulthood , whereas vimentin immunoreactivity decreased after E 35 , becoming limited to a few end feet and fibers in the adult , mainly in the tegmentum . ^^^ Thus , in developing Gallotia midbrain a shift from vimentin containing to GFAP containing intermediate filaments begins around E 36 or E 37 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using antibodies against glial fibrillary acidic protein ( GFAP ) , vimentin and S 100 protein and peroxidase anti peroxidase techniques . ^^^ Material from brains perfusion fixed with formaldehyde remained positive for astrocytic GFAP , but was negative for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunolabeling patterns for vimentin and glial fibrillary acidic protein ( GFAP ) were studied in five rhesus monkeys that had undergone retinal detachment or detachment and reattachment . ^^^ Anti vimentin and anti GFAP labeling intensity increased in Mller cells after 2 days of detachment . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Sections of seven human epiretinal membranes of diverse pathologic origin were labeled with antibodies to cellular retinaldehyde binding protein ( CRALBP ) and two intermediate filament proteins : glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Radial glia were positive for vimentin and glial fibrillary acidic protein ( GFAP ) throughout the age range . ^^^ Germinal eminence cells were focally vimentin positive from 15 weeks , focally GFAP positive from 17 weeks , and negative for all NFP and for NSE . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The usefulness of 1 naphthol as substrate for horseradish peroxidase ( HRP ) in immunohistochemistry was studied using the peroxidase antiperoxidase ( PAP ) and avidin biotin complex ( ABC ) methods in the demonstration of glial fibrillary acidic protein ( GFAP ) , vimentin , carbonic anhydrase C ( CA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial markers , namely antibodies to glial fibrillary acidic protein ( GFAP ) , vimentin , galactocerebroside ( GC ) , and 08 antigen , were used to study the occurrence and location of neuroglial cells in adult rabbit retinae . ^^^ Mllerian glia were vimentin positive , lacked detectable amounts of GFAP , and were found in all parts of the rabbit retina . ^^^ Astroglia showing GFAP and vimentin immunoreactivity were absent from most of the superior and inferior retina , being found only in the myelinated area of the ganglion cell axon bundles , the medullary rays . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Light and electron microscopical studies of the tumours confirmed their glial nature , however immunocytochemical staining for the astrocyte specific markers glial fibrillary acidic protein ( GFAP ) and glutamine synthetase ( GS ) and for vimentin suggest that the tumours are composed mainly of poorly differentiated neoplastic glial cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
With one exception , these lines expressed IFs only of the vimentin type ; neurofilament peptides , desmin and GFAP were not detected . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial reaction has been studied in the rat by the immunohistochemical demonstration of glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) in two experimental conditions . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
On the main antigens ( S 100 protein , GFAP , Vimentin , Neuronal specific Enolase , neurofilaments , myelin basic protein , carboanhydrase C , FVIII / RAg , laminin , fibronectin ) the most important findings are described and critically considered . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cultures were immunostained for GFAP and vimentin . mRNAs to GFAP , vimentin , alpha and beta tubulin , and actin also were detected by in situ hybridization with biotinylated cDNA probes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ependymal cells express GFAP and vimentin filaments , whereas plexus epithelium simultaneously contains neurofilaments , cytokeratins and vimentin , a phenomenon not previously observed in normal cells in vivo . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In analogy to experimental results previously obtained with vimentin and neurofilament triplet proteins , the intermediate filament ( IF ) proteins desmin and glial fibrillary acidic protein ( GFAP ) were also found to have high capacities to associate with nucleic acids . ^^^ In general , desmin and GFAP were found to be similar to vimentin and neurofilament proteins in their nucleic acid binding properties . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Sixteen choroid plexus tumors ( CPTs ) have been investigated for the localization of different immunocytochemical markers of epithelial and nonepithelial nature , namely , simple epithelial type cytokeratins , vimentin , glial fibrillary acidic protein ( GFAP ) , a panepithelial antigen defined by the lu 5 monoclonal antibody ( lu 5 antigen ) , S 100 protein , and epithelial membrane antigen ( EMA ) . ^^^ In 7 of these 14 cases , in addition to cytokeratins and vimentin , the neoplastic cells were shown to coexpress GFAP , which is not synthesized by their normal cell counterpart . ^^^ The appearance of GFAP immunoreactivity in CPTs might be related to an ependymal differentiation of the neoplastic cells , because normal ependyma and ependymomas constantly coexpress GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
New aspects are gained from immunohistochemical investigations using mono and polyclonal antibodies against intermediate filaments ( GFAP , vimentin , cytokeratins ) , neuron specific enolase ( NSE ) , protein S 100 , carcinoembryonic antigen ( CEA ) , myelin associated glycoprotein ( Leu 7 ) , synaptophysin and nuclear proliferating antigen ( Ki 67 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ependymal cells contain GFAP and vimentin filaments , whereas epithelial plexus cells express cytokeratin , vimentin and neurofilament 200 KD . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Coexpression of GFAP , keratin and vimentin in tumor cells in aspirates is an unusual feature , so far demonstrated only in pleomorphic adenomas . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The peroxidase anti peroxidase technique was used for localization of glial fibrillary acidic protein ( GFAP ) and vimentin ( VM ) in 19 ependymal tumors in order to determine if a unique pattern of intermediate filament ( IF ) expression could be demonstrated . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin and glial fibrillary acidic protein ( GFAP ) were uniformly negative in normal parotids but showed variable ( often strong ) reactivity with some cells in chondroid , myxoid and solid areas of PA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Furthermore , vimentin and , focally , glial fibrillary acidic protein ( GFAP ) were detected in the cystic epithelia . ^^^ By double labelling , coexpression of cytokeratin and vimentin , GFAP and cytokeratin , and GFAP and vimentin could be demonstrated . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin , but not keratin , neuron specific enolase ( NSE ) , glial fibrillary acidic protein ( GFAP ) , neurofilament ( NF ) , desmin and Factor 8 related antigen ( FVIII AG ) , demonstrated positivity in the cytoplasm of the neoplastic cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These intermediate filament protein bands on Western blots were characterized as 3 cytokeratin like proteins ( 49 , 55 , and 58 kDa ) , one vimentin like protein ( 53 kDa ) , two distinct glial fibrillary acidic protein ( GFAP ) like proteins ( 60 and 67 kDa ) , and 3 neurofilament proteins ( 73 , 175 , and 200 kDa ) by evaluation of their crossreactivities with specific antibodies directed against the mammalian forms of these proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This work , based on Golgi impregnations , transmission electron microscopy and immunocytochemistry , demonstrates that the intermediate filaments found in the radial gliocytes of the adult newt spinal cord are both vimentin and glial fibrillary acidic protein ( GFAP ) structures . ^^^ They were extensively immunolabelled with both anti vimentin and anti GFAP monoclonal antibody conjugates . ^^^ The presence of GFAP confirms the astroglial character of the radial glia in urodeles ; the existence of vimentin suggests that the spinal cord of the adult animal retains immature astroglia , which should express enlarged capabilities of adaptation . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Sixteen oligodendrogliomas , twelve mixed oligoastrocytomas and ten anaplastic oligodendrogliomas were immunotested by the peroxidase antiperoxidase ( PAP ) method with anti GFAP serum , anti S 100 serum and anti MBP ( Myelin basic protein ) serum and by the avidin biotin peroxidase complex ( ABC ) method with anti vimentin serum and ant Leu 7 monoclonal antibody . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Each tumor was studied with monoclonal antibodies to keratin , vimentin , desmin , 200 kd neurofilament protein , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry on formalin fixed , paraffin embedded tissue was used to identify : S 100 protein ( S 100 ) ; neuron specific enolase ( NSE ) ; chromogranin A ( CHA ) ; serotonin ( SER ) ; synaptophysin ( SYN ) ; cytokeratin ( CK ) ; neurofilament ( NF ) ; desmin ( DES ) ; vimentin ( VIM ) ; and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Desmin , GFAP , vimentin , peripherin and the lightest neurofilament protein ( NF L ) were cleaved into carboxy and amino terminal halves by N chlorosuccinimide at their unique trytophan residue . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of glial fibrillary acidic protein ( GFAP ) and vimentin was investigated immunohistochemically in 104 experimental gliomas induced by transplancental application of ethylnitrosourea ( ENU ) in CDF rats . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In both cell types the immunostaining for glial fibrillary acidic protein ( GFAP ) , S 100 protein and vimentin was strongly positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical examination showed GFAP and vimentin positive cells were all reactive astrocytes around capillaries and near calcifications . ^^^ No tumor cells contained GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Formalin fixed , paraffin embedded sections of 59 ultrastructurally confirmed nerve sheath tumors ( NSTs ) that included 27 benign schwannomas , five neurofibromas , and 27 malignant schwannomas were studied by the avidin biotin peroxidase complex method using antibodies directed against glial fibrillary acidic protein ( GFAP ) , keratin , S 100 protein , vimentin , and desmin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Three cases had expression of both glial fibrillary acid protein ( GFAP ) and vimentin , but only one of the myoepitheliomas contained muscle specific actin . ^^^ Using double labeled immunofluorescent microscopy of one reticular variant and the plasmacytoid myoepithelioma , there was individual tumour cell co expression of GFAP and vimentin focally in the plasmacytoid myoepithelioma , but co expression of cytokeratins 13 , 16 and GFAP were not noted in either case . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The 17 markers studied included vimentin ( VIM ) , Factor 8 related antigen ( FVIIIR : Ag ) , blood group antigens A , B , and H , Ulex 1 lectin ( Ulex ) , Alkaline Phosphatase ( Alk P ) , neurofilament protein ( NF ) , glial fibrillary acidic protein ( GFAP ) , S 100 protein ( S 100 ) , nerve growth factor receptor ( NGFR ) , muscle specific actin ( MSA ) , desmin ( Des ) , monoclonal keratin ( MKER , including Cam 5 . 2 and AE1 / 3 ) , epithelial membrane antigen ( EMA ) , and chromogranin ( Chrom ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression of intermediate filament proteins was studied in human developing spinal cord using immunoperoxidase and double label immunofluorescence methods with monoclonal antibodies to vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ Vimentin was found in the processes of radial glial cells in 6 week embryos , while GFAP appeared in vimentin positive astroglial cells at 8 10 weeks . ^^^ GFAP and vimentin were present in approximately equal amounts in differentiating astrocytes in 23 week spinal cord . ^^^ In 30 week fetuses , astrocytes reacted strongly for GFAP , while both the reaction intensity and the number of vimentin positive cells fluctuated predominantly in the grey matter . ^^^ No clear cut transition from vimentin to GFAP was noticed during the development of astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Whereas the endocrine cells only expressed cytokeratins 8 and 18 , the folliculo stellate cells exhibited a reactivity for cytokeratins 7 , 8 , 18 and 19 as well as for GFAP and vimentin . ^^^ Whereas in may cystic epithelial cells including the `` pseudo follicles ' ' a triple expression of cytokeratin , vimentin and GFAP could be observed , only some basal cells of squamous epithelial nests coexpressed cytokeratin and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Comparative studies with antibodies against vimentin , GFAP , and fibronectin suggest that anti GDN recognizes cells associated with the olfactory system , but not exclusively the olfactory neurons themselves . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The avidin biotin complex method was employed using polyclonal immune sera against glial fibrillary acidic protein ( GFAP ) , S 100 protein and vimentin , and monoclonal antibodies against neurofilaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Primary antibodies were directed against alpha fetoprotein ( AFP ) , alpha 1 antitrypsin ( AAT ) , lysozyme ( LYS ) , carcinoembryonic antigen ( CEA ) , human chorionic gonadotropin ( HCG ) , glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) , epithelial membrane antigen ( EMA ) , hepatitis B surface antigen ( HbSA ) , lactoferrin ( LF ) , desmin ( DES ) , vimentin ( VIM ) , and keratin ( KER ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antisera to glial fibrillary acidic protein ( GFAP ) and vimentin were used to elucidate the distribution of these intermediate filament proteins in postnatal marmoset brains of various ages . ^^^ The ependyma of the lateral ventricles was unique in being equally immunoreactive for both GFAP and vimentin at all ages . ^^^ In neonates , vimentin immunoreactivity greatly exceeded that of GFAP and was located primarily in radial glia in the subependymal plate of the anterior cerebrum . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Mucoepidermoid carcinomas were generally positive for cytokeratin and showed double expression for cytokeratin and vimentin in 31 . 1 % and triple expression for cytokeratin , vimentin and GFAP in 24 . 1 % . ^^^ Acinic cell carcinomas were generally positive for cytokeratin and in single cases showed double expression for cytokeratin and vimentin and triple expression for cytokeratin , vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cryostat sections of two old plaques removed at autopsy from the spinal cord of a 62 year old man with multiple sclerosis of 24 year duration were studied by indirect immunofluorescence with antibodies to neurofilament proteins , glial fibrillary acidic protein ( GFAP ) , glial hyaluronate binding protein ( GHAP ) , vimentin and laminin . ^^^ Both multiple sclerosis plaques were positive for GFAP and vimentin , but negative for GHAP , while astrocytes in myelinated spinal cord white matter stained with both GFAP and GHAP antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In response to loss of axonal contact , vimentin expression by Schwann cells within the distal nerve stump increased , becoming a predominant intermediate filament protein of the cytoskeleton while glial fibrillary acid protein ( GFAP ) expression decreased . ^^^ This reversal in the prominence of intermediate filament protein was maintained until the onset of axonal reinnervation , at which point expression of GFAP increased and vimentin decreased . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In a wide range of canine tissues the immunoreactivity with commercially available antisera against intermediate filament antigens viz . vimentin , desmin , glial fibrillary acidic protein ( GFAP ) and neurofilament proteins , was studied . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These results suggest that the increase of GD 3 and upper band GM 3 in long term cultured astrocytes might be related to the appearance of small processes showing strong reactivity against GFAP and vimentin during astrocyte subculture . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial markers , namely , vimentin , glial fibrillary acidic protein ( GFAP ) , and glycogen , as well as accumulation of axon borne horseradish peroxidase ( HRP ) , were used to visualize radial glial cells in the developing opossum superior colliculus ( SC ) and to follow changes in young astrocytes of the superficial layers . ^^^ Vimentin , GFAP , and glycogen are relatively abundant in elements of the median ventricular formation ( MVF ) , which persists at least as late as weaning time , i . e . , postconception day 103 , postnatal day 90 ( PND 90 ) . ^^^ Radial profiles and end feet in the remaining collicular sectors ( main radial system , MRS ) are also vimentin positive but show little or no glycogen or anti GFAP staining . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Spinal cord sections from rats sensitized to develop experimental allergic encephalomyelitis ( EAE ) were immunostained with antibodies against glial fibrillary acidic protein ( GFAP ) , carbonic anhydrase , and vimentin , to see whether the latter two antigens could be detected in GFAP positive reactive astrocytes . ^^^ In the latter , GFAP and carbonic anhydrase were colocalized with vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical identification of keratin proteins ( TK , KL 1 and PKK 1 ) , vimentin , myosin , S 100 protein ( using polyclonal antiserum ) and S 100 alpha and beta subunits , glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) , lactoferrin , and lysozyme was made in myoepitheliomas , myoepithelial adenomas , and clear cell adenomas of salivary gland origin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Central nervous system ( CNS ) tissue from young myelin deficient ( md ) mutant rats and male littermate controls was immunostained with antibodies against the following glial cell antigens : glial fibrillary acidic protein ( GFAP ) , glutamine synthetase , vimentin , the Yb isozyme of glutathione S transferase ( Yb ) and carbonic anhydrase . ^^^ The most dramatic differences between astrocytes in the md and normal animals were revealed with anti GFAP and anti vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In all these species , the SCO ependymocytes possess vimentin as an intermediate filament , but never express GFAP or neurofilament proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin expression was detected by immunofluorescence microscopy using a monoclonal antibody ( V 9 ) against porcine lens vimentin in combination with either antiserum against the astroglial marker , glial fibrillary acidic protein ( GFAP ) , or with antiserum against the oligodendroglial marker , galactocerebroside ( GC ) . ^^^ Process bearing cells maintained in 10 % serum exhibited heterogeneity in their expression of GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Commercially available antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin were used in conjunction with our own antibodies ( 3F11 , MA 1 and 4D6 ) to label astrocytes in the adult albino rabbit retina . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The positions of the eight peripherin gene introns correspond to the intron patterns of desmin , vimentin , and GFAP , with one example of intron sliding . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antisera to vimentin , epithelial membrane antigen ( EMA ) , keratin , S 100 protein , carcinoembryonic antigen ( CEA ) , desmin , factor 8 , Ulex europeaus , and glial fibrillary acidic protein ( GFAP ) were utilized . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cell type specific immunofluorescence staining was examined by using antibodies directed against glial fibrillary acidic protein ( GFAP ) and vimentin , two astroglial markers ; neuron specific enolase ( NSE ) and neurofilament ( NF ) , two neuronal markers , and galactocerebroside ( GC ) , an oligodendroglial marker . ^^^ It was found that the cellular maturation in aggregates is characterized by distinct developmental increases in immunoreactivity for GFAP , vimentin , NSE , NF , and GC , and by a subsequent decrease of vimentin positive structures in more differentiated cultures . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Among the antigens detected in periretinal membranes are GFAP , keratin , vimentin , actin , fibronectin , collagen , and macrophage antigen . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical localization of glial fibrillary acidic protein ( GFAP ) and vimentin in the subcommissural organ of the Mongolian gerbil ( Meriones unguiculatus ) . ^^^ Antibodies against glial fibrillary acidic protein ( GFAP ) , vimentin , neurofilament proteins and cytokeratins were used . ^^^ Only GFAP and vimentin were detected in the non specialized diencephalic ependyma and in the ependymocytes of the SCO . ^^^ In contrast to the homogeneous vimentin labeling , the SCO was particularly immunoreactive for GFAP in its lateral aspects and in the supra and precommissural parts . ^^^ The coexpression of GFAP and vimentin in a subclass of SCO ependymocytes was demonstrated on differentially immunostained semithin sections . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cells with similar cytological features in the pars distalis or the intermediate zone were found to share common immunoreactivities against GFAP and the presumable FS cell markers vimentin and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astroglial features were characterized by high levels of glial fibrillary acidic protein ( GFAP ) and vimentin , which are the major intermediate filament proteins present in astrocytes at an early culture stage . ^^^ In long term cultured ( greater than 7 months ) astrocytes , vimentin and GFAP were increased in process bearing cells . ^^^ These results suggest that the increase of ganglioside GD 3 in long term cultured astrocytes may be related to the appearance of multistellate cells showing strong reactivity against GFAP and vimentin during development over a specified period in culture . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry with respect to proteins of the cytoskeleton and other cell markers showed positive tumour cell immunoreactions for vimentin and S 100 , but not for GFAP , Leu 7 , Leu M 1 and MBP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Outgrowing cells were identified and characterized by ( 1 ) morphological criteria using light and electron microscopy , ( 2 ) in vivo [ 3H ] thymidine labeling combined with subsequent in vitro autoradiography , ( 3 ) immunocytochemistry for vimentin , GFAP , Fc and complement receptors , MHC antigens , laminin , fibronectin , factor 8 related and 04 antigen as well as lectin histochemistry , and ( 4 ) functional in vitro tests . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin was demonstrated in four cases and was not always co expressed with GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Coronal sections of the cerebral wall from developing ferrets ( newborn to adult ) were double stained with antibodies to vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ A small population of immature astrocytes in the cortical plate was double labeled for GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This labelling consists of a histochemical visualization of anterogradely transported horseradish peroxidase ( HRP ) , within outgrowing rat corticospinal tract fibers and a double immunofluorescence labelling against glial fibrillary acidic protein ( GFAP ) and vimentin , being specific markers for astrocytes and their precursors , respectively . ^^^ Thereafter , double immunofluorescence labelling is carried out on the same section by adding simultaneously polyclonal anti GFAP and monoclonal anti vimentin which are then visualized with antisera conjugated with tetramethylrhodamine isothiocynate ( TRITC ) and fluorescein isothiocyanate ( FITC ) respectively as markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using modified peroxidase antiperoxidase ( PAP ) immunoperoxidase ( IP ) techniques , they evaluated the immunoreactivity to glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) , neurofilament ( NF ) , chromogranin ( CG ) , and vimentin ( VM ) . ^^^ All nine teratomas were immunoreactive for both GFAP and NSE , one was reactive for NF , and five ( 56 % ) were immunoreactive for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Among glial fibrillary acidic protein ( GFAP ) , keratin and vimentin , FS cells showed greatest affinity to vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The authors ' results indicated the following : ( 1 ) The inner cell layers of tubuloglandular components have distinct epithelial features with cytokeratin ( CK ) , carcinoembryonic antigen ( CEA ) , and epithelial membrane antigen ( EMA ) positivity . ( 2 ) The outer cell layers had negative results for muscle specific actin ( MSA ) , desmin , CEA , EMA , and CK ( if antibody AE1 / 3 was used ) and had positive results for vimentin , S 100 protein , neuron specific enolase ( NSE ) , and in three cases for glial fibrillary acidic protein ( GFAP ) . ( 3 ) The stromal cells and , to a lesser extent , the solid nests expressed immunophenotypes similar to those of the outer cell layers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Results of immunocytochemical studies demonstrated that in flat astrocytes which develop after 3 weeks of culturing in serum supplemented medium , the IF containing vimentin and glial fibrillary acidic protein ( GFAP ) are concentrated around the nucleus and dispersed in an irregular fashion throughout the cytoplasm . ^^^ Under both culture conditions , vimentin and GFAP are resistant to extraction with low salt buffer containing nonionic detergent , indicating that the different cytoplasmic distribution of IF is unrelated to the solubility properties of vimentin and GFAP . ^^^ However , electrophoretic and immunoblot analyses of detergent extracted proteins from parallel cultures demonstrate that neither the amount nor the solubility of GFAP and vimentin are changed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Eighteen granular cell tumors from various sites were examined with antisera directed against protein S 100 , neuron specific enolase ( NSE ) , alpha 1 antichymotrypsin , and alpha 1 antitrypsin , glial fibrillary acidic protein ( GFAP ) , lysozyme , factor 8 related antigen , myoglobin and vimentin , as well as with a monoclonal antibody ( lu 5 ) directed against a panepithelial marker . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We studied : ( 1 ) the cellular composition of the cultures by using two astroglial markers : vimentin and glial fibrillary acidic protein ( GFAP ) ; ( 2 ) the spontaneous and forskolin stimulated somatostatin release . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Normal , reactive , and neoplastic astrocytes express two types of intermediate filament ( IF ) proteins , namely glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the non epithelial intermediate filament branch , the ancestor of neurofilament proteins and the common ancestor of desmin , vimentin , and glial fibrillary acidic protein ( GFAP ) diverged first . ^^^ By evolutionary criteria , the intermediate filament protein recently discovered in neuronal cells does not belong to the neurofilament family but is more closely related to desmin , vimentin , and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The presence of heat shock proteins of three different molecular weights , and of vimentin , was identified by the use of monoclonal antibodies and of GFAP by a suitable polyclonal antibody coupled in each instance with immunofluorescence microscopy or immuno electron microscopy . ^^^ Changes in the expression of HSP , GFAP and vimentin can all be regarded as part of the Mller cell response to heat shock conditions . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To test the possibility that the astrocytes in these cultures arose from glial precursor cells , we followed the expression of ganglioside GD 3 , galactosylceramide ( GC ) , glial fibrillary acidic protein ( GFAP ) , and vimentin in the cultures . ^^^ With time , the oligodendrocytes remained GD 3 / GFAP / vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Double labeled fluorescence immunocytochemistry showed that in the brainstem of twitcher mutant , GFAP and vimentin were coexpressed in the majority of astrocytes . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The development of glia in the hippocampal formation of normal and hypothyroid rats was studied using immunocytochemical staining for either glial fibrillary acidic protein ( GFAP ) or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial fiber pattern in the developing chicken cerebellum : vimentin and glial fibrillary acidic protein ( GFAP ) immunostaining . ^^^ The possible relation between glial fibers and the formation of longitudinal granule cell migration patterns that occur in the cerebellar anlage of the chicken was investigated by immunocytochemistry of vimentin ( monoclonal antibody ) and glial fibrillary acidic protein ( polyclonal antibody against GFAP , PGF ) on fixed and unfixed brain tissues . ^^^ However , no specific topographic relation of vimentin and GFAP to the migration pattern of granule cells was observed . ^^^ In adults , Bergmann fibers and astroglia were stained with vimentin antiserum and not with GFAP antiserum . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cytoskeletal preparations of the purified astrocyte cultures from mature bovine brain contain both vimentin and glial fibrillary acidic protein ( GFAP ) , but vimentin is by far the major intermediate filament protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes showing GFAP and vimentin immunoreactivity were absent from most of the horse retina . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunoblot experiments indicated that the 51K / 6 . 0 protein stained with antiserum to glial fibrillary acidic protein ( GFAP ) and the 57K / 5 . 7 protein stained with antibodies to vimentin . ^^^ These experiments suggest that the membrane receptors present on astroglia function , in part , to regulate phosphorylation of the intermediate filament proteins GFAP and vimentin . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
By labelling astrocytes with radioactive methionine , we demonstrate increases of GFAP and vimentin synthesis within 48 h of exposure to dbcAMP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Receptor agonists that increase cyclic AMP levels in cultured astroglia have been shown to increase 32P labeling of the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin in these cells . ^^^ Time course experiments indicated that 6 8 h were required to reach steady state 32P labeling of both GFAP and vimentin . ^^^ Treatment with forskolin ( 10 microM ) after steady state 32P labeling increased GFAP and vimentin phosphorylation fourfold and twofold , respectively , and also induced a morphological change from polygonal to process bearing cells within 20 30 min of drug addition . ^^^ Cells incubated in media containing brain extract ( 30 % ) for 24 h at 37 degrees C and then 3 h at 23 degrees C underwent changes from polygonal to process bearing cells with no apparent increase in the phosphorylation of either GFAP or vimentin . ^^^ These results suggest that forskolin stimulation of GFAP and vimentin increases net number of phosphates associated with these intermediate filament proteins and that the resulting increase in phosphorylation can be dissociated from morphological changes . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of vimentin ( VIM ) has been histochemically investigated in 53 cerebral tumors and compared in gliomas to that of glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of intermediate filament ( IF ) proteins was studied in 71 cases of malignant human astrocytoma and in 17 cases of reactive gliosis , using immunocytochemical techniques with polyclonal and monoclonal antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ In all cases of astrocytoma , varying in degree of malignancy from grade 1 to grade 4 , co expression of GFAP and vimentin was found . ^^^ No change in vimentin or GFAP IF expression with increasing anaplasia was seen . ^^^ In addition astrocytic cells in reactive gliosis showed simultaneous expression of GFAP and vimentin . ^^^ Vimentin was found to be located in a more juxta nuclear position , whereas GFAP immunoreactivity showed a more intense staining of the cellular processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Measurement of the amount of vimentin indicated that GFAP was induced under conditions of low vimentin concentration . ^^^ They indicate a shift from vimentin to GFAP synthesis by an as yet unknown mechanism . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Increases in vimentin were also noted , following a time course similar to that of GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin and glial fibrillary acidic protein ( GFAP ) represent the principal constituents of intermediate filaments found in astrocytes . ^^^ In contrast to vimentin GFAP transition which occurs during glial development in situ , vimentin coexists with GFAP in cortical astrocytes allowed to differentiate in culture . ^^^ To examine whether culture conditions or proliferative activity of the cells is responsible for the expression of vimentin , we generated cultures of GFAP positive , vimentin negative astrocytes isolated from 26 day postnatal rat brain cortices . ^^^ Within 3 days in culture , all astrocytes accumulated GFAP in hypertrophic cell bodies and many began to express vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies were directed against antigenic markers , the expressions of which overlapped in time : GD 3 ganglioside in immature neuroectodermal cells ; vimentin in radial glia ; glial fibrillary acidic protein ( GFAP ) in astrocytes ; and carbonic anhydrase ( CA ) and galactocerebroside ( GC ) in oligodendrocytes . ^^^ At E 15 radial glia were stained with the anti vimentin antibody but were negative for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial markers , namely antibodies to glial fibrillary acidic protein ( GFAP ) , vimentin , galactocerebroside ( GC ) , 04 antigen , and 08 antigen , were used to study the development of neuroglial cells in the postnatal rabbit retina . ^^^ Few vimentin labeled astrocytes , situated in the nerve fiber layer , were detectable at birth , but of these few many were also GFAP positive . ^^^ The number of GFAP positive astrocytes , all of which co labeled with vimentin antibodies , increased during the following days . ^^^ From postnatal day ( P ) 9 10 onward , the vimentin labeling seen in GFAP positive astrocytes began to decline . ^^^ In the adult rabbit most GFAP positive astrocytes were only weakly vimentin stained ; some astrocytes even lacked detectable amounts of vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Polyclonal antiserum against vimentin and monoclonal antibodies to glial fibrillary acidic protein ( GFAP ) and to the 200 kD neurofilament triplet protein were used . ^^^ In the human retina , Mller ' s cells coexpressed vimentin and GFAP in most instances , probably as a reactive phenomenon . ^^^ The tumors contained , however , vimentin and GFAP in the stromal cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A comparative study of immunoreactivity of GFAP , vimentin , S 100 protein , and neurofilament in 38 schwannomas and 18 neurofibromas . ^^^ Immunoreactivity of glial fibrillary acidic protein ( GFAP ) in 38 schwannomas and 18 neurofibromas was evaluated and compared with the reactivity of vimentin , S 100 protein , and neurofilament protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The antigenic expression of glial fibrillary acidic protein ( GFAP ) , vimentin , S 100 protein and neurofilament triplex polypeptide was examined in cultured glial ( Mller ) cells from adult rabbit retina using immunocytochemical techniques . ^^^ Using a double labelling technique , 70 90 % of cultured cells showed positive labelling for GFAP and vimentin although the staining intensity was stronger in the case of the latter . ^^^ Immunoelectron microscopy confirmed that GFAP and vimentin were localised along the intermediate glial filaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical study of developing rat embryo localization of vimentin , GFAP , neurofilament protein within rat embryo central nervous system ] . ^^^ We have stained the specimens for vimentin in accordance with ABC method and for GFAP in accordance with PAP method . ^^^ Summarizing of these results , we can display first : immature cells composing of neural plate are vimentin positive at day 9 , second : NFP positive fibers and cells appear at day 13 , third : NFP positive cells are vimentin positive , too , forth : GFAP positive fibers appear at day 18 and they are vimentin positive , too . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To assess the cytogenesis of the central nervous system we studied the spinal cord and the cerebrum in 11 human embryos and fetuses of gestation age 7 25 weeks immunohistochemically using anti vimentin , anti neurofilament protein ( NFP ) , anti neuron specific enolase ( NSE ) , anti glial fibrillary acidic protein ( GFAP ) , anti S 100 protein , anti Leu 7 and anti myelin basic protein ( MBP ) antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
No tumor cells were positive , however , for neurofilaments , vimentin , keratin , desmin , GFAP , S 100 protein , ACTH , and somatostatin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemical investigation was performed on a large series of adult , neonatal , and fetal respiratory tract cartilages to ascertain their immunoreactivity for glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Simultaneous immunoreactivity for GFAP and vimentin has also been ascertained in chondrocytes and in perichondrial stellate or elongated cells of the 25 chondromatous hamartomas investigated . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunostaining for GFAP and vimentin overlaps in astrocytes trapped within neoplasms , in astrocytes at the tumor periphery , and in the peritumoral zone . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
One cell type expressed the astrocyte intermediate filament , GFAP , in addition to vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of cytokeratin ( 40 52 kD ) , carcinoembryonic antigen ( CEA ) , vimentin , neuron specific enolase ( NSE ) , S 100 protein , glial fibrillary acidic protein ( GFAP ) were investigated in 20 cases of pleomorphic adenoma of the salivary glands and 10 cases of normal salivary glands , in order to analyze and correlate the antigens ' expressions with the probably histogenetic mechanisms of the various histopathological differentiations in pleomorphic adenoma of the salivary glands and their probably original cells in normal salivary glands . ^^^ In both normal glands and pleomorphic adenomas , the duct lining cells were immunoreactive for cytokeratin , CEA and had both cytoplasmic and nuclear S 100 positivity ; The myoepithelial cells of the normal glands as well as the periduct cells , epithelial nests / cords , squamous metaplasia and the stellate / spindle / cartilaginous cells in the myxomatous chondroid areas of the pleomorphic adenoma contain immunoreactive vimentin , NSE , S 100 proteins and GFAP , and lesser amounts cytokeratin ( 40 52 kD ) / CEA . ^^^ The peripheral neurons and axons of the autonomic nervous system were identified by vimentin , NSE , S 100 proteins and GFAP . ^^^ The combination of epithelial cytokeratin and nervous system related vimentin , NSE , S 100 and GFAP immunostaining in myoepithelium of the normal glands and in all component elements ( particularly the periduct cells ) of pleomorphic adenoma reflects pleomorphic adenoma of the salivary glands is an epithelial tumor , the probably original cells or the probably histogenetic mechanisms of the various histopathological differentiations is correlated not only with `` duct acinar myoepithelial cells ' ' but also with the neuroectoderm in the normal salivary glands . ' ' . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of glial fibrillary acidic protein ( GFAP ) , vimentin and fibronectin ( Fn ) was studied in cells cultured from human glioma and fetal brain by indirect immunofluorescence ( IIF ) microscopy and multiple labelling experiments . ^^^ In the primary cultures a major part ( 20 % 70 % ) of the cells usually displayed both GFAP and vimentin and the rest of the cells only vimentin . ^^^ A prominent variation in GFAP and vimentin fluorescence intensity sometimes made interpretation of double IIF stainings difficult . ^^^ However , occasional GFAP positive cells appeared vimentin negative in primary glioma cultures , whereas in fetal brain primary cultures cells that were preferentially GFAP positive also showed at least a weak vimentin immunoreactivity . ^^^ As judged by double IIF , the GFAP positive cells were usually Fn negative , while the Fn positive cells were vimentin positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies of the tumor demonstrated glial fibrillary acid protein ( GFAP ) immunoreactivity in areas of glioma and focal vimentin and actin immunoreactivity in areas of sarcoma . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical study of human brain tumors with vimentin and astroprotein ( GFAP ) ] . ^^^ Distributions of two different subclasses of intermediate filaments , vimentin and glial filaments , were studied immunohistochemically in human brain tumors using specific antiserum to each protein subunit , vimentin and astroprotein ( GFAP ) , Surgical specimens ( 5 meningiomas , 4 ependymomas , 5 benign astrocytomas , 5 anaplastic astrocytomas and 7 glioblastomas ) were fixed in 95 % ethanol or ethanol acidic acid ( 95 : 5 ) and embedded in paraffin Avidin biotin peroxidase complex ( ABC ) method ( Vectastain ) was carried out on 6 microns thick paraffin sections . ^^^ All meningioma cells were negative for astroprotein ( GFAP ) and positive for vimentin . ^^^ Ependymoma cells showed various patterns of immunoreaction for astroprotein ( GFAP ) but were invariably positive for vimentin . ^^^ In anaplastic astrocytomas , population of astroprotein ( GFAP ) positive cells decreased and vimentin positive cells increased . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Quantitation of GFAP and vimentin demonstrated that enhanced fluorescence occurs without an increase in the level of intermediate filament proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The NG 2 labeled cells were not labeled with antibodies against glial fibrillary acidic protein ( GFAP ) , vimentin , or S 100 protein , intracellular markers for astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial filaments contain vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ The question of how glial filaments change morphologically according to the expression of vimentin and / or GFAP has remained unclear . ^^^ F6B3 tumor expressed GFAP and vimentin in large quantities . ^^^ G10A10 tumor expressed plenty of vimentin but only a little of GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used antibodies against the HNK 1 epitope , which is shared between natural killer cells and the nervous system , glial fibrillary acidic protein ( GFAP ) , vimentin , neurofilaments , S 100 protein , neuron specific enolase ( NSE ) and myelin basic protein ( MBP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The nature of astrocytic cells in rat gliomas induced by ethylnitrosourea ( ENU ) was studied by means of immunohistochemically demonstrating glial fibrillary acidic protein ( GFAP ) , vimentin and beta subunit of S 100 protein ( S 100 beta ) on paraffin sections . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
As for intermediate filament , tumor cells in both solid and cystic portions showed positive immunoreactivity for cytokeratin and GFAP , and not for vimentin and neurofilament . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to study which classes of intermediate filaments ( IF ) comprise the IF of human trabecular cells , we undertook immunocytochemical investigations on cultured human trabecular cells using anti keratin , anti vimentin , anti desmin and anti glial fibrillar acidic protein ( GFAP ) rabbit sera . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
From these results , it was suggested that astrocytes in the molecular layer of the cortex and the white matter adjacent to the lesion proliferated in response to the injury and expressed vimentin transiently , then acquired GFAP , and that astrocytes in the IInd to VIth layers of the cortex became reactive astrocytes without mitosis . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Hemosiderin isolated from frozen cerebellum contained ferritin , GFAP , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have examined the development of astrocytes in the albino rabbit retina , using antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This protein is distinct from GFAP ( MW 50 kD ) and vimentin ( MW 55 kD ) , the two core proteins of 10 nm IFs known to be expressed in the above types of macroglia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The possible existence of homologs of mammalian GFAP and vimentin in the goldfish retina was also explored . ^^^ Antibodies directed against mammalian GFAP and vimentin labeled the Mller fibers and the cone horizontal cells , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of GFAP , S 100 , NSE , vimentin , cytokeratin , fibronectin and desmoplakin was investigated . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Intermediate filaments and anaplastic change of ENU induced gliomass : immunohistochemical study with vimentin and astroprotein ( GFAP ) . ^^^ To study the expression of two different subclasses of intermediate filaments in ethylnitrosourea induced rat cerebral gliomas , the number of cells immunopositive for each subunit protein , vimentin and astroprotein ( GFAP ) , was quantitatively analyzed . ^^^ Vimentin is a subunit protein of non specific intermediate filaments which appear transiently in immature glial cells , while astroprotein ( GFAP ) is a subunit protein of glial filaments , normally expressed in mature astrocytes . ^^^ Accordingly , the vimentin / GFAP ratio [ the number of vimentin positive cells divided by the number of astroprotein ( GFAP ) positive cells ] was increased from 0 . 23 to 1 . 86 , and from 0 . 26 to 1 . 85 , respectively , as oligodendrogliomas and mixed gliomas become anaplastic . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NF kinase also phosphorylated two additional brain cell cytoskeletal elements : GFAp and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tumor from eight enucleated eyes was analyzed by immunohistochemistry , using a panel of specific antibodies including interphotoreceptor retinoid binding protein ( IRBP ) , S antigen ( S Ag ) , opsin , neuron specific enolase ( NSE ) , glial fibrillary acidic protein ( GFAP ) , laminin , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The extent of homology between the goldfish 58K intermediate filament proteins themselves and to rat optic nerve vimentin and glial fibrillary acidic protein ( GFAP ) was investigated . ^^^ The results show that the goldfish intermediate filament proteins share with vimentin and GFAP a 40K chymotrypsin resistant core fragment . ^^^ In addition , structural variability is observed between the goldfish intermediate filament proteins and vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Conversely , glia in brain territory , that is , in the optic tract and elsewhere in the CNS , lack the fish vimentin , but express GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both glial fibrillary acidic protein ( GFAP ) and vimentin antibodies gave a fibrillar cytoplasmic staining of the cells , and double immunofluorescence experiments showed the presence of both types of intermediate filaments in the same cells . ^^^ GFAP and vimentin could also be located to typical coiling perinuclear bundles after vinblastine treatment of the cultures . ^^^ In the detergent resistant , adherent cytoskeletons of the glioma cells , both GFAP and vimentin persisted as fibrillar cytoplasmic arrays . ^^^ Electrophoretic analysis , combined with the immunoblotting method , revealed that the two major detergent resistant cytoskeletal polypeptides of the cells , with molecular weights of 51 kD and 58 kD , were GFAP and vimentin , respectively . ^^^ On the other hand , neither GFAP nor vimentin were detected in the detergent extracts of the glioma cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Five cases of alveolar soft part sarcoma , 5 cases of granular cell tumor , and 6 cases of paraganglioma were investigated immunohistochemically to examine the expression of tissue specific intermediate filaments ( cytokeratin , vimentin , desmin , and glial fibrillary acidic protein ( GFAP ] , actin , myoglobin , and nervous tissue markers ( S 100 protein , neuron specific enolase , and Leu 7 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using the peroxidase antiperoxidase technique , all specimens were stained for GFAP , vimentin , S 100 and neuron specific enolase ( NSE ) . ^^^ The myelination glia in the eight normal brains stained positively for GFAP but not for vimentin . ^^^ Thus , GFAP positivity combined with vimentin negativity in both neoplastic and immature oligodendroglia suggests that GFAP positivity in oligodendrogliomas may reflect the transient expression of this intermediate filament by immature oligodendroglia . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cytoskeletal protein preparations showed increases in GFAP as well as in vimentin in the brainstem , spinal cord , and sciatic nerve . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Triple expression of GFAP , vimentin and cytokeratins in papillary meningioma and metastasizing renal carcinoma . ^^^ To ascertain the specificity of such unexpected immunoreactivities for GFAP and to exclude possible crossreactivities with other intermediate filament ( IF ) proteins , a panel of different antibodies was used for immunocytochemistry with the peroxidase antiperoxidase ( polyclonal antisera ) or labeled biotin avidin ( monoclonal antibodies ) techniques : two monoclonal and four polyclonal anti GFAP , three monoclonal and one polyclonal anti cytokeratins ( CK ) , and two monoclonal anti vimentin ( VIM ) antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Fetal human brain and leptomeningeal specimens embedded in paraffin were analyzed immunohistochemically for the distribution of glial fibrillary acidic protein ( GFAP ) , vimentin , factor 8 related antigen , fibronectin , laminin , type 4 collagen , and procollagen 3 . ^^^ Only GFAP and vimentin identified astrocytes and radial glia in the developing human brain ; fibronectin , laminin , and the collagen types were immunolocalized largely to the leptomeninges and to the cerebral vasculature . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Clonal growth and ratio of progenitor cells to oligodendrocytes was measured over the next two weeks by phase microscopy and double immunofluorescence labeling for the specific markers A2B5 , GC ( galactocerebroside , a surface marker for differentiated oligodendrocytes ) , O 4 ( a glycolipid marker of oligodendrocytes and some progenitors ) , GFAP ( an astrocyte specific intermediate filament protein ) and vimentin . ^^^ In type A clones , some cells became GC positive multipolar oligodendrocytes , while other multipolar cells remained GC negative for days and expressed A2B5 and O 4 , but not GFAP or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical examination was performed with polyclonal antibodies to keratin , alpha fetoprotein ( AFP ) , desmin , neurofilament , glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) , and S 100 protein , and with monoclonal antibodies to vimentin , 45 to 54 kd cytokeratin ( CKER ) , and carcinoembryonic antigen ( CEA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Human corneal endothelial cells and keratocytes were immunohistochemically examined using antisera against neuronal tissue antigens , namely , S 100 protein and neuron specific enolase ( NSE ) , and intermediate filaments such as vimentin , neurofilament , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using the peroxidase antiperoxidase ( PAP ) technique , we examined 35 primary brain tumors for expression of vimentin and GFAP . ^^^ Both low grade and high grade astrocytomas contained vimentin positive and GFAP positive cells . ^^^ Medulloblastomas and oligodendrogliomas were negative for both vimentin and GFAP , while meningiomas contained scattered areas of vimentin positive cells . ^^^ These results suggest that the expression of vimentin and GFAP is mostly confined to glial derived tumors , and that vimentin can potentially be a useful marker for distinguishing undifferentiated GFAP negative glial tumors and mesenchymal tumors from primitive neuroectodermal tumors . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Additionally and unexpectedly they reveal among each other lower sequence identity than the three non neuronal IF proteins GFAP , desmin , and vimentin where the last two are particularly closely related . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Three contained vimentin , two neurofilaments and one vimentin , neurofilaments and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In cultures started from whole brains of 12 day old rat embryos , fibronectin was expressed within 24 h in culture by all the cells with morphology of flat astrocytes , positive for vimentin but negative for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The proteins were visualized by Coomassie blue staining and immunoblotting with specific antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ At 15 weeks of development , only trace amounts of GFAP were observed and vimentin was the predominant intermediate filament protein . ^^^ In contrast , there was more than twice as much GFAP as vimentin in the adult optic nerve . ^^^ The results also showed that the ratio of GFAP to vimentin is higher in the optic tract than in the optic nerve during development , whereas in adult tissue , the ratio is the same for the two regions . ^^^ The amounts of both GFAP and vimentin were the same for both the normal and involved optic nerve . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical study of ethylnitrosourea induced rat gliomas with vimentin and astroprotein ( GFAP ) ] . ^^^ In the present communication , distribution of vimentin filaments in rat glial tumors was investigated and compared with that of glial filaments by using specific antiserum to each protein subunit , vimentin and astroprotein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cellular levels of the intermediate filament ( IF ) proteins , vimentin and glial fibrillary acidic protein ( GFAP ) , and actin , insoluble in Triton 10 100 , were examined by polyacrylamide gel electrophoretic analysis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cells failed , however , to express glial fibrillary acidic protein ( GFAP ) but did express the intermediate filament protein vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The antibodies raised against Purkinje fibre skeletin reacted with all three polypeptides of the `` neurofilament triplet ' ' , with glial fibrillary acidic protein ( GFAP ) , with smooth muscle desmin and also slightly with some prekeratin subunits and with endothelial vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Six chordomas , nine chondrosarcomas , and three myxopapillary ependymomas of the filum terminale were evaluated immunohistochemically for the expression of intermediate filament proteins by the use of monospecific antibodies against intermediate filament proteins of keratin , vimentin , and glial fibrillary acidic protein ( GFAP ) type . ^^^ Chondrosarcomas showed strong vimentin positivity , whereas ependymomas were positive for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The rat spinal cord contains glial fibrillary acidic protein ( GFAP ) , and the rat optic nerve contains vimentin and GFAP , in addition to the neurofilament triplet . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ten non neoplastic pituitary glands and 22 pituitary adenomas producing different hormones were studied by immunofluorescence microscopy as well as peroxidase antiperoxidase and biotin avidin techniques on frozen sections and formalin fixed , paraffin embedded material using antibodies to cytokeratin , vimentin , GFAP , neurofilament protein and different pituitary hormones . ^^^ Vimentin and GFAP reactivity was confined to agranular folliculo stellate cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the EAE rat all cytoskeletal proteins , including the neurofilaments , vimentin , microtubules , GFAP and actin , showed increased uptake of radioactive amino acids . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Intermediate sized filament proteins ( IFP ) are tissue specific in that antibodies to keratin , vimentin , desmin , glial fibrillary acidic protein ( GFAP ) and the neurofilament proteins can distinguish between cells of epithelial and mesenchymal origin as well as of myogenic and neural origin respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In sciatic nerves of adult mice , C 1 but not M 1 antigen is found in vimentin and glial fibrillary acidic protein ( GFAP ) positive Schwann cells . ^^^ In all cases vimentin positive structures outnumber GFAP or C 1 antigen positive ones . ^^^ At birth , C 1 antigen and vimentin are expressed in sciatic nerves , but GFAP is not yet detectable . ^^^ A similar augmentation is also observed for vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
When cultured astrocyte proteins were blotted , the antibody bound to both GFAP and vimentin , but no GFAP staining was observed in any of the tissue section staining . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A monoclonal antibody to vimentin ( RBA 1 ) and a polyclonal antiserum to glial fibrillary acidic protein ( GFAP ) were used in double labeling experiments to examine astrocyte intermediate filaments in development and wounding . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The results are that IgG monoclonal antibodies identify an astrocyte GFAP specific epitope and IgM monoclonal antibodies cross react with a common epitope to GFAP and vimentin . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In all cell lines studied indirect immunofluorescence stainings revealed bright arrays of vimentin type intermediary filaments but no desmin , cytokeratin , glial fibrillary acidic protein ( GFAP ) , or neurofilament proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antigenic markers characteristic of astrocytes and their differentiative states ( i . e . , glial fibrillary acidic protein ( GFAP ) , vimentin , and M 1 and C 1 antigens ) were investigated in the pineal gland of mouse and rat using double immunolabeling techniques . ^^^ Subpopulations of vimentin positive pineal astrocytes were also GFAP and C 1 antigen positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Presence and distribution of S 100 protein ( S 100 ) , neuron specific enolase ( NSE ) , cytokeratin polypeptides , glial fibrillary acidic protein ( GFAP ) , vimentin , actin , lysozyme and pituitary hormones ( prolactin , hGH , ACTH , beta FSH , beta LH , beta TSH , alpha subunit ) in folliculo stellate cells ( FSC ) were studied in seven normal human pituitary glands and 28 pituitary adenomas using peroxidase antiperoxidase and the avidin biotin immunohistochemical techniques . ^^^ Approximately 5 % of the cells of the adenohypophysis were agranular , non hormone producing FSC most of which showed a conspicuous and strong reaction with S 100 antibodies but some were , in addition , GFAP and vimentin positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Relative amounts of the proteins differed , in that low density cells contained large amounts of cytoskeletal actin relative to the intermediate filament ( IF ) proteins vimentin and GFAP , whereas high density cells contained relatively less actin and more IF proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We previously reported that the cytoskeleton of rat astrocytes in primary culture contains vimentin , glial fibrillary acidic protein ( GFAP ) , and actin . ^^^ In short term incorporation experiments vimentin , GFAP , and actin in the Triton insoluble fraction were labeled within 5 min after exposure of the cultures to radioactive leucine . ^^^ The turnover of filament proteins studied by following the decay of radioactivity from prelabeled vimentin , GFAP , and cytoskeletal actin displayed biphasic decay kinetics for all three proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A case of esthesioneuroblastoma , the pathological diagnosis of which almost always causes great difficulties , was investigated ultrastructurally , biochemically , and immunohistologically , using antibodies against the five known types of intermediate filaments [ keratin , vimentin , desmin , glial fibrillary acidic protein ( GFAP ) and neurofilaments ] . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The specimens extracted from developing human spinal cords , gestational age of 15 weeks , 30 weeks and 35 weeks , were prepared with cryostat for the investigation of anti vimentin mouse monoclonal antibody and of GFAP rabbit serum . ^^^ We have stained the specimens for vimentin in accordance with ABC method , for GFAP in accordance with PAP method . ^^^ On gestational age of 15 weeks , the fibers radially oriented from the central canal to pial surface were positive for vimentin and GFAP . ^^^ On gestational age of 30 weeks , radially oriented fibers were positive for vimentin and GFAP . ^^^ From results above , we concluded : 1 ) Radial glial cells in developing human spinal cord were positive for vimentin and GFAP . 2 ) The show weaker staining for vimentin in process of gestational age . 3 ) They differentiate to astrocyte . 4 ) They appear in earlier stage than suggested by previous reports . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunofluorescence microscopy showed that approximately 80 % of the cells were positive for glial fibrillary acidic protein ( GFAP ) and greater than 80 % were stained with an antiserum to the molecular weight 58 , 000 fibroblast intermediate filament protein ( vimentin ) . ^^^ The electrophoretic peptide patterns of the 58 , 000 protein were similar to those of vimentin isolated from NIL 8 fibroblasts , and the patterns of the 51 , 000 protein were similar to those of GFAP isolated from rat spinal cord . ^^^ We conclude that the major proteins of cytoskeleton preparations from cultured primary astrocytes are vimentin ( 58 , 000 ) , GFAP ( 51 , 000 ) , and actin ( 42 , 000 ) , and that our data show no obvious structural relationship among them . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical examination for glial fibrillary acidic protein ( GFAP ) revealed remarkable positive reactivity in the perinuclear cytoplasm , but no immunoreactivity for vimentin or Leu 7 was found . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies against glial fibrillary acidic protein ( GFAP ) , cytokeratins ( AE 1 , CK5D , AE 3 ) , S 100 protein , epithelial membrane antigen ( EMA ) , vimentin , neuron specific enolase ( NSE ) , neurofilaments protein ( NF ) and prealbumin , were used . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin and GFAP immunoreactivity in developing and mature neural microvessels . ^^^ The expression of the cytoskeletal filaments vimentin and GFAP has been analyzed by immunocytochemical techniques in endothelial cells , pericytes , and astrocyte perivascular endfeet of microvessels of chicken optic tectum and cerebellum during embryonic development and in adulthood . ^^^ Astrocyte processes closely associated with the vessel wall were vimentin stained in the 11 i . d . cerebellum and vimentin and GFAP reactive in 15 i . d . tectum . ^^^ The results indicate that intermediate filament expression in the cells of the brain microvasculature is developmentally regulated , and suggest that the vimentin to GFAP transition in perivascular astrocytes parallels the vessel wall maturation . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies to glial fibrillary acidic protein ( GFAP ) , S 100 protein , epithelial membrane antigen ( EMA ) , carcinoembryonic antigen ( CEA ) , keratin ( KER ) and vimentin ( VIM ) were used . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Non glial intermediate filament ( IMF ) proteins , as well as glial fibrillary acidic protein ( GFAP ) and vimentin , were studied by immunohistochemistry in 24 gliomas including low grade astrocytoma , pleomorphic xanthoastrocytoma , anaplastic astrocytoma , glioblastoma , oligodendroglioma , ependymoma and ependymoblastoma , which were fixed in ethanol and embedded in paraffin . ^^^ All tumors had GFAP positive neoplastic cells and vimentin was also found in all the tumors except one oligodendroglioma . ^^^ DM P might crossreact with GFAP and vimentin . ^^^ We conclude that , in addition to GFAP and vimentin , gliomas express several types of non glial IMF proteins . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of immunoreactivity of bone morphogenetic protein ( BMP ) , the glycosaminoglycans chondroitin 4 sulphate ( C4SPG ) , chondroitin 6 sulphate ( C6SPG ) , dermatan sulphate ( DSPG ) and keratan sulphate proteoglycans ( KSPG ) , cytokeratin ( K8 . 12 ) , vimentin , glial fibrillary acidic protein ( GFAP ) , actin , desmin , S 100 protein and neuron specific enolase ( NSE ) in mixed tumour of the skin was investigated using immunohistochemical methods using monoclonal ( MoAb ) and polyclonal antibodies ( PoAb ) . ^^^ Vimentin , S 100 protein , GFAP and NSE , but not actin and desmin , were distribute in the outer tumour cells and modified myoepithelial cells in chondroidally changed tissue . ^^^ First , outer or basal tumour cells in mixed tumour of the skin is characterized by the presence of positive immunoreactivity for BMP , KSPG , vimentin , cytokeratin K8 . 12 , S 100 protein , GFAP and NSE , and second , there is a matrix of chondroidally changed tissue containing the reaction products of C4SPG , C6SPG , DSPF and KSPG . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A total of 14 cases of clear cell carcinoma of salivary glands were evaluated by immunohistochemical methods using monoclonal antibodies to cytokeratin ( K1 . 1 and K8 . 12 ) , vimentin , S 100 alpha and beta subunits , neuron specific enolase ( NSE ) , glial fibrillary acidic protein ( GFAP ) , MAM 3 and MAM 6 antigens and proliferating cell nuclear antigen ( PCNA ) , as well as polyclonal antibodies to lysozyme ( Ly ) , lactoferrin ( la ) and Alpha 1 antichymotrypsin ( alpha 1 Ach ) . ^^^ Cytokeratin KL 1 and K8 . 12 was present in few tumor cells with almost negligible to strong reaction in all cases , vimentin in 6 , GFAP in 5 cases with multiple expression of cytokeratin K8 . 12 , vimentin and GFAP in 5 cases . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A spectrum of human astrocytomas has been examined by immunofluorescence microscopy employing antibodies to several IF structural subunit proteins ( GFAP , vimentin , and keratins ) and an IF associated protein , IFAP 300kDa . ^^^ These proteins occupy unique temporal niches in the cytogenesis of the astrocytic cells : keratins in cells of the neuroectoderm ; vimentin and IFAP 300kDa in radial glia and immature glia ; GFAP in mature astrocytes ; and vimentin in some mature astrocytes . ^^^ In agreement with previous reports , our immunofluorescence studies have revealed both GFAP and vimentin in all astrocytoma specimens . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical analysis of 9 choroid plexus papillomas ( CPPs ) and 3 choroid plexus carcinomas ( CPCs ) using a panel of antibodies against glial fibrillary acidic protein ( GFAP ) , cytokeratin ( CK ) , epithelial membrane antigen ( EMA ) , S 100 protein , vimentin ( vim ) , and neuron specific enolase ( NSE ) is presented . ^^^ Focal positivity was observed for GFAP in 11 , vimentin in 7 , cytokeratin in 2 and EMA in 3 cases . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Commercially available antibodies directed against high and low molecular weight keratins ( RCK 102 and NCL 5D3 ) , vimentin , desmin , glial fibrillary acidic protein ( GFAP ) , neurofilament ( NF ) proteins and muscle actin ( HHF 35 ) were used in the avidin biotin peroxidase complex ( ABC ) technique on formalin fixed paraffin wax embedded tumour tissue samples . ^^^ In addition , basal / myoepithelial cells expressed vimentin and muscle actin in both species , and GFAP was found in some feline basal / myoepithelial cells . ^^^ Feline mammary gland carcinoma cells reacted with RCK 102 ( 89 % ) , NCL 5D3 ( 62 % ) , vimentin ( 76 % ) and GFAP ( 30 % ) antibodies , while human mammary gland carcinoma cells reacted with RCK 102 ( 95 % ) , NCL 5D3 ( 100 % ) and vimentin ( 13 % ) antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The IF subgroups ( cytokeratins , Cks ; neurofilaments , NFs , vimentin , glial fibrillary acidic proteins , GFAP ; desmin ) displayed characteristic staining patterns . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry was used to detect changes in the expression of glial fibrillary acidic protein ( GFAP ) and vimentin up to 24 h after injury . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Possible differentiation mechanisms were investigated in a glioblastoma multiform cell line ( GL 15 ) presenting an undifferentiated phenotype with weak glial fibrillary acidic protein ( GFAP ) and strong vimentin ( VIM ) expression . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The inclusions were negative for antibodies to cytokeratin Cam5 . 2 , epithelial membrane antigen ( EMA ) , vimentin , S 100 , neurofilaments , glial fibrillary acidic protein ( GFAP ) and carcinoembryonic antigen ( CEA ) and there was no post Congo red apple green birefringence to indicate amyloid . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At no time during development are the lateral radial cells immunopositive for the glial fibrillary acidic protein ( GFAP ) ; however , shorter , vimentin positive astrocytic profiles can be seen in the tectum around the time the radial fibers have been withdrawn , suggesting that at least some radial cells are transformed into astrocytes that will colonize the mature colliculus . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Embryo and adult rat spinal cord sections immunostained for S 100 protein , vimentin , glial fibrillary acidic protein ( GFAP ) , or phosphorylated 160 kDa neurofilament protein ( NF ) , were used to study glial participation in nerve tissue assembly . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Proteins examined included glial fibrillary acidic protein ( GFAP ) , vimentin , tubulin , and actin . ^^^ GFAP and vimentin : At 1 day after detachment , there was an aggregation of intermediate filaments in the endfoot of Mller cells . ^^^ The increase in cytoskeletal proteins GFAP , vimentin , and tubulin within the retinal Mller cells after detachment may help to stabilize this cell type as it hypertrophies during glial scar formation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
DFP administration resulted in mCANP activity inhibition in sciatic nerve and significant decrease in total NF H , phosphorylated NF H , vimentin , GFAP , tubulin , and tau . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both giant and smaller cells excluding neuronal , endothelial and infiltrative cells were positive for GFAP , vimentin , and alpha 1 anti chymotrypsin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
From the large number of cases ( 53 ) diagnosed in Geneva over a period of 10 years , we decided to do a retrospective study of these tumours with regard to their immunohistological phenotypes , using the following markers : S 100 protein ( S 100 ) , Neuron specific enolase ( NSE ) , Vimentin , Chromogranin , Glial fibrillary acidic protein ( GFAP ) , Keratin , Epithelial membrane antigen ( EMA ) , Desmin , Smooth muscle alpha actin . 100 % of tumours were positive for S 100 , and 90 % for NSE , which confirms the neurogenic origin . 70 % were Vimentin positive , with however a variable intensity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Under these conditions the contamination with non neuronal cells was minimized to less than 5 % , as evidenced by immunohistochemical methods using the well established cell marker proteins : neuron specific enolase ( NSE ) as neuronal marker , and vimentin and glial fibrillary acidic protein ( GFAP ) as astroglial markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical stains for smooth muscle antigen ( SMA ) , S 100 protein , glial fibrillary acidic protein ( GFAP ) , vimentin and desmin were performed with the peroxidase antiperoxidase method on paraffin sections . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the normal retina , S 100 protein , glial fibrillary acidic protein ( GFAP ) and vimentin were detected in astrocytes and / or Mller cells . ^^^ In all retinoblastomas , strong NSE expression and weak bcl 2 expression was observed in almost all tumour cells and synaptophysin was localized in rosette forming cells , while tumour cells were devoid of S 100 , GFAP , vimentin and neurofilament protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Toward this end , vimentin ( VIM ) , glial fibrillary acidic protein ( GFAP ) and vasoactive intestinal peptide ( VIP ) immunoreactivity were investigated in SCN radial glia , astrocytes and neurons , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astroglial reactions , monitored by immunohistochemical staining for the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) , and the normal plasma constituent immunoglobulin G ( IgG ) , showed an initial and generalized astroglial immunoreactivity for IgG , which paralleled the initial and transient microglial reactions , while the reactive changes in GFAP and VIM immunohistochemistry paralleled the protracted , degeneration specific reactions with regard to timing , strength , and distribution . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The astrocytic reaction in the rat after brain injury has been studied immunohistochemically for intermediate filaments ( GFAP and vimentin ) , also with double staining procedures , and for markers of proliferation ( BrdU and PCNA ) . ^^^ GFAP positive reactive astrocytes appeared around the lesion , where they were vimentin positive and at a distance . ^^^ BrdU positive astrocytes represented a minor fraction of GFAP and vimentin positive astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In both operated md and normal littermates crush lesions contained degenerating astrocytes as well as reactive astrocytes in which the intermediate filaments of the perikarya failed to stain immunocytochemically for GFAP , vimentin , desmin , and a common determinant of intermediate filaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At 8 10 weeks gestational age ( GA ) , progenitor cells and their immediate progeny in the rhombencephalic ventricular zone expressed vimentin and nestin and , to a lesser extent , microtubule associated protein 5 ( MAP 5 ) and glial fibrillary acidic protein ( GFAP ) , but not the low affinity nerve growth factor receptor ( NGFR ) . ^^^ Finally , radial glia were positive for vimentin , GFAP , and nestin from 8 weeks GA to 8 months postnatal . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistological expressions of the cytokeratin ( CK ) subclass , vimentin , glial fibral antigen protein ( GFAP ) , alpha smooth muscle actin , and S 100 protein were investigated in pleomorphic adenoma of the parotid gland . ^^^ Vimentin , S 100 protein , and GFAP , which were not detected in the normal parotid gland , were observed in pleomorphic adenoma . ^^^ Therefore , the expression of vimentin , S 100 protein , GFAP , and CK 1 in pleomorphic adenoma is related to oncogenesis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies for bromodeoxyuridine ( BrdU ) , glial fibrillary acidic protein ( GFAP ) , vimentin , and laminin were employed to assess the infiltration of inflammatory cells , proliferation activity of cells , and reaction of astrocytes and mesenchymal cells , respectively . ^^^ The number of inflammatory cells , number of BrdU positive cells , area and number of vimentin positive astrocytes , and the area of GFAP positive astrocytes were quantitatively analyzed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The majority of these cells exhibit a strong immunoreactivity with glial antibodies ( GFAP , vimentin , S 100 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical evaluation of the tumour showed a positive immunoreactivity for vimentin , alpha and beta subunits of S 100 protein , neurone specific enolase ( NSE ) , substance P , met enkephalin and chromogranin but cytokeratins , desmin , actin , myosin , glial fibrillary acidic protein ( GFAP ) and calcitonin gene related peptide ( CGRP ) were negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Specific antibodies against vimentin and glial fibrillary acidic proteins ( GFAP ) identified neuroglia . ^^^ High numbers of cells in the female and male SCN immunoreacted with vimentin in neonates and GFAP in adults . ^^^ Astrocytes containing vimentin or GFAP were few in number in the SDApc and surrounding areas in neonates and adults , respectively . ^^^ There was a sex difference in the numerical density of both vimentin and GFAP positive cells in the SCN . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This shows that the loss of GFAP intermediate filaments is not compensated for by the up regulation of other intermediate filament proteins , such as vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The spindle shaped subpopulation typically formed small clusters of cells that were immunostained by antibodies to GFAP , vimentin , and low affinity NGF receptor ( LNGFR ) and demonstrated a low incidence of dye coupling ( 30 % ) , whereas the fiber like cells aligned themselves along their long axes , immunostained for gp140trkA , LNGFR , and anti alpha smooth muscle actin and showed extensive ( 100 % ) and diffuse dye spread to numerous other contiguous fiber like cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
From 16 h , reactive astrocytosis , defined as an increase in the number and size of cells expressing GFAP and vimentin , was observed surrounding the neocortical lesion site . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the adrenal medulla , supporting cells in noradrenaline ( NA ) cell regions showed similar and intense immunoreactivities to the three glial marker proteins , S 100 protein , glial fibrillary acidic protein ( GFAP ) and vimentin , forming a network , while those in adrenaline ( A ) cell regions were less numerous and their immunoreactivity was positive to S 100 protein but negative to GFAP and , at least in part , negative to vimentin . ^^^ In the pineal gland , supporting cells in the stalk and the proximal region of the body portion formed a network and showed immunoreactivities to the three antibodies , while those in the distal region of the body portion were less numerous and their immunoreactivity was positive to S 100 protein and vimentin , but negative to GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
It was found by immunoblotting analysis that even the reconstituted microfilament like filaments , which had been purified by affinity chromatography with heavy meromyosin subfragment 1 ( S 1 ) conjugated Sepharose , contained vimentin and glia fibrillary acidic protein ( GFAP ) besides actin , inferring the interaction between microfilament and glia filament . ^^^ The filaments ( 9 10 nm thick ) reconstituted from the glia filament extract were composed of actin and other minor components in addition to vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Phenotypic characteristics of B 2 cells were investigated by immunolabeling with cell type specific markers for oligodendrocytes ( O 4 and galactocerebroside ( GalC ) ) , astrocytes ( glial fibrillary acidic protein ( GFAP ) ) , and immature neuroectodermal cells ( vimentin ) . ^^^ Under this culture condition , vimentin was identified in all of the B 2 cells , GFAP in 7 % , and O 4 and GalC in less than 1 % of the cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The different retinal cells developed normal reactivity for the intracellular markers rhodopsin , neurofilament 160 kDa , vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ependyma : phylogenetic evolution of glial fibrillary acidic protein ( GFAP ) and vimentin expression in vertebrate spinal cord . ^^^ The phylogenetic evolution was studied of both glial fibrillary acidic protein ( GFAP ) and vimentin expression in the ependyma of the adult vertebrate spinal cord . ^^^ The importance of the immunohistochemical procedure , the cellular nature of GFAP positive ependymal cells and the relationship between tanycytes and ependymocytes are discussed , as well as GFAP and vimentin expression . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Co distribution of vimentin and glial fibrillary acidic protein ( GFAP ) was sparsely observed in the astrocyte like cells , which suggested an immature glial phenotype . ^^^ Oligodendroglia like cells and their intercellular neuropil like structures expressed beta tubulin isotype 3 , synaptophysin and NSE , in addition to the expressions of vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In particular , exposure to 100 pM gp 120 for 30 min depressed the phosphorylation of vimentin and glial fibrillary acidic protein ( GFAP ) induced by isoproterenol in rat cortical astrocyte cultures . ^^^ We found that chronic ( 3 day ) treatment of the cells with 100 pM gp 120 before exposure to isoproterenol was substantially more effective than acute treatment in depressing the stimulatory effect of the beta adrenergic agonist on vimentin and GFAP phosphorylation . 3 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Postnatal development of glial fibrillary acidic protein , vimentin and S 100 protein in monkey visual cortex : evidence for a transient reduction of GFAP immunoreactivity . ^^^ We studied the postnatal maturation of astrocytes in the primary visual cortex of Callithrix jacchus using antibodies against GFAP , vimentin and S 100 protein as immunohistochemical markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Here we demonstrate that these glial fibers , which are immunoreactive to Rat 401 , vimentin , and glial fibrillary acidic protein ( GFAP ) antibodies , are seen in adult rats . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP , introduced as a marker of neurotoxicity and Vimentin , which is expressed by immature or reactive astrocytes were investigated . ^^^ We found a dose related increase of GFAP positive radial glia and star shaped Vimentin positive astrocytes in the high lead group . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Effects of chronic low level lead exposure on the expression of GFAP and vimentin mRNA in the rat brain hippocampus analysed by in situ hybridization . ^^^ GFAP and Vimentin were chosen as glialtypic markers for neurotoxicity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Several additional cell markers have been included : rhodopsin , rod ( alpha transducin , neuron specific enolase ( NSE ) , synaptophysin ( SYN ) , cone specific opsins , vimentin , cellular retinaldehyde binding protein ( CRALBP ) , glial fibrillary acidic protein ( GFAP ) , rat major histocompatibility antigen class 2 ( MHC 2 ) and a rat macrophage marker ( Ox 42 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression of vimentin and GFAP and development of the retina in the trout ] . ^^^ The intermediate filament proteins , vimentin and glial fibrillary acidic protein ( GFAP ) were visualized by an indirect immunohistochemical method . ^^^ Results show that both vimentin and GFAP are early expressed in the developing retina and , particularly in the Mller cells , a coexpression of vimentin and GFAP is observed from embryonic to adult stages . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
On the other hand , no PSA IR was detected on axons immunoreactive to somatostatin or to corticotropin releasing hormone projecting throughout the median eminence , or on glial cell bodies and processes immunoreactive for glial fibrillary acidic protein ( GFAP ) or for vimentin dispersed throughout the median eminence and the neural lobe . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Since astrocytes are closely associated with motor neurons , antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin were used as independent monitors of astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We investigated the activation of Ca2+ / calmodulin dependent protein kinase 2 ( CaM kinase 2 ) via stimulation of glutamate receptors and subsequent phosphorylation of vimentin and glial fibrillary acidic protein ( GFAP ) in cultured rat cortical astrocytes . ^^^ In the experiments using 32P labeled astrocytes , the phosphorylation of vimentin and GFAP as well as autophosphorylation of CaM kinase 2 were found to be stimulated after the exposure to glutamate . ^^^ It was concluded by two dimensional phosphopeptide analysis that the increased phosphorylation of vimentin and GFAP observed in intact cells were due to the activation of CaM kinase 2 by glutamate . ^^^ These results suggest that glutamate can activate CaM kinase 2 through stimulation of both the metabotropic and non N methyl D aspartate receptors , and that the concomitant phosphorylation of vimentin and GFAP may in turn regulate the functions of intermediate filament proteins in intact astrocytes . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We find that the alpha crystallins dramatically inhibit the in vitro assembly of GFAP and vimentin in an ATP independent manner . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to determine the cell type that was affected , sections from brains of MK 801 treated rats were processed for 3H thymidine autoradiography combined with immunohistochemistry for the marker of radial glia , vimentin , and the marker of mature astrocytes , glial fibrillary acidic protein ( GFAP ) . ^^^ No change in the numbers of 3H thymidine labeled vimentin immunoreactive or GFAP immunoreactive cells was observed in the dentate gyrus with MK 801 treatment , indicating that glia are not primarily affected by NMDA receptor blockade . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To study the effect of recombinant human IL 1 beta ( rhIL 1 beta ) on astrocyte morphology , glial fibrillary acidic protein ( GFAP ) and vimentin expression , and actin organization , we conducted a systematic survey using dissociated human fetal astrocyte cultures . ^^^ These `` activated ' ' astrocytes displayed intense GFAP and vimentin immunoreactivity in the small perikarya and processes . ^^^ In contrast , the large , flat astrocytes in control cultures showed diffuse pale immunoreactivity for GFAP and vimentin . ^^^ To quantify the changes in GFAP and vimentin content with IL 1 beta stimulation , densitometric analyses of northern and western blots were performed . ^^^ Northern blot analysis of IL 1 beta stimulated astrocytes revealed a transient , marked decrease in steady state levels of mRNA for GFAP , vimentin , and microtubule associated protein 4 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Western blot analysis revealed a remarkable increase of GFAP expression in GFC 6 5 and GFC 6 6 compared with that in NeoC 6 and C 6 , in contrast to similar vimentin expression in all cell lines . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the large tumor cells were distinctly positive for cytokeratin , vimentin , GFAP , the melanoma marker NKI C 3 , NSE , and S 100 . ^^^ The small tumor cells were only slightly positive for GFAP , NKI C 3 , NSE , and S 100 but they were negative for cytokeratin and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Another type 3 IF protein , vimentin , colocalizes with GFAP in immature , reactive or radial glia , thereby indicating that vimentin has an important role in the build up of the glial architecture . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry was performed with antibodies specific for neuron specific enolase ( NSE ) , synaptophysin ( SYN ) , cone specific opsins , rhodopsin , rod alpha transducin , S antigen , vimentin , cellular retinaldehyde binding protein ( CRALBP ) and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Concomitant with the enhanced astrocytic GFAP , astrocytes were swollen and expressed immunoreactivity to vimentin and gamma aminobutyric acid ( GABA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Indirect immunofluorescence staining using anti vimentin and anti glial fibrillary acidic protein ( GFAP ) antibodies revealed that the orderly array of the intermediate filament system was severely disturbed . ^^^ Western blot analysis of cellular extracts was carried out to determine the protein levels of GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
An immunohistochemical study including nervous system associated protein ( S 100 ) , glial fibrillary acidic protein ( GFAP ) , neurofilament protein ( NF ) , laminin , fibronectin , vimentin , factor 8 ( von Willebrand factor ) related antigen , and the nuclear antibody Ki 67 was performed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To clarify the fate of extravasated serum protein and tissue reaction following blood brain barrier breakdown in mature and immature brain , we produced cryogenic injury in the cortices of adult and post natal day 2 rats , and immunohistochemical examination with GFAP , vimentin and albumin , endogenous tracer of serum proteins , antibodies . ^^^ However , fibrotic scar , cyst formation and GFAP , vimentin positive astrocytosis were main features in mature brain , fusion of adjoining cortical plate without scar and astrocytosis was typical in the immature brain . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Routine histological studies with immunohistochemistry using antibodies for BrdU , GFAP , vimentin and laminin were carried out to assess infiltration of inflammatory cells , formation of edema , cell proliferation , and reactivity of astrocytes and mesenchymal cells . ^^^ The number of inflammatory cells , number of BrdU positive cells , area and number of vimentin positive astrocytes , and the area of GFAP positive reactive astrocytes were quantitatively analyzed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present immunohistochemical study , the distribution and differentiation of glial and neuronal cells in bovine fetal brains ( age range : between 1 2 and 7 8 months ) was examined using antibodies against nervous system specific proteins , i . e . , glial fibrillary acidic protein ( GFAP ) , vimentin , neuron specific enolase ( NSE ) and a neurofilament protein subunit ( NF200kD ) . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Unlike the vimentin motif in which even conserved sequence changes affect filament assembly , the GFAP consensus sequence , MERRRITS ARRSY , can be replaced by a completely unrelated sequence ; namely , the heptapeptide , MVRANKR , derived from the lambda cII protein . ^^^ Sequences similar to the GFAP RP box motif are also to be found in vimentin , desmin and peripherin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression and assembly characteristics of carboxy and amino terminal deletion mutants of glial fibrillary acidic protein ( GFAP ) , an astrocyte specific type 3 intermediate filament protein , were examined by transient transfections of either vimentin positive or vimentin negative variants of human adrenocarcinoma derived SW 13 cell lines . ^^^ Whereas complete deletion of the C terminal tail domain of GFAP results in the formation of polymorphic aggregates , both intranuclear and cytoplasmic in self assembly experiments , efficient co assembly of these tail less GFAP mutants with vimentin can be achieved as long as the KLLEGEE sequence at the C terminal end of the rod domain is preserved . ^^^ All N terminal deletion mutants of GFAP share the same phenotype of diffuse cytoplasmic staining when expressed in vimentin negative SW 13 cells . ^^^ Although co assembly with vimentin can still be achieved with completely head less GFAP , preservation of some of the head domain greatly enhanced the efficiency . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The aim of this study was to characterize the cytoskeletal intermediate filaments , glial fibrillary acidic protein ( GFAP ) , and vimentin in normal and lead treated rats , and to compare the astroglial response in the cerebellum and the hippocampus two regions with great susceptibility to the toxic effects of lead . ^^^ Experiments combined light and electron microscopy immunohistochemistry using antibodies to GFAP and to vimentin , and conventional transmission electron microscopy techniques . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present study , the neoplastic myoepithelial cells in five pleomorphic adenomas of parotid gland were labeled with actin , myosin , S 100 protein , glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Intermediate filament expression by retinal macroglia was studied in cryostat sections and in retinal flatmounts using avidin biotin peroxidase immunolabeling of antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ In flatmounts , vimentin distribution was similar in the three groups , but GFAP labeling revealed hypertrophic astrocytes , which were absent from normal retinae , in 17 % of aged retinae and 55 % of AMD affected retinae . ^^^ CONCLUSIONS : Variation in GFAP and vimentin expression in retinal macroglia is affected by increasing age , and a distinctive variation of intermediate filament expression in retinal macroglia is associated with the pathogenesis of AMD . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To exclude other primary or metastatic neoplasms of the skull base possibly sharing the same cytological picture , additional direct smears were immunostained with antibodies specific for cytokeratin ( CK ) , vimentin ( VIM ) , S 100 protein ( S100P ) , carcinoembrionic antigen ( CEA ) , epithelial membrane antigen ( EMA ) , glial fibrillary acidic protein ( GFAP ) , CD 68 antigen ( KP 1 ) and with the ' panepithelial ' antibodies B72 . 3 and Ber EP 4 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin failed to immunoreact with any cells in day 5 CNS cultures . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The non astrocytic cells which proliferate in the rat brain after the induction of an area of necrosis have been characterized and counted by means of combined in vivo bromodeoxyuridine ( BrdU ) administration and immunohistochemical demonstration of glial fibrillary acid protein ( GFAP ) , vimentin , Ricinus communis agglutinin 120 ( RCA 1 ) , Griffonia simplicifolia B 4 isolectin ( GSI B 4 ) , keratan sulphate ( KS ) , carbonic anhydrase C ( CA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our immunohistochemical findings , using a panel of antibodies ( GFAP ) , vimentin , keratins , F 8 related antigen , muscle specific actin , KP 1 and HMB 45 ) , support its undifferentiated nature . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Developmental distribution of GFAP and vimentin in the Brazilian opossum brain . ^^^ We have utilized immunoblotting and immunohistochemistry to characterize the developmental profiles of glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) in the brain of the Brazilian opossum , Monodelphis domestica . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Two different types of intermediate filaments , vimentin ( VIM ) and glial fibrillary acidic protein ( GFAP ) were studied by immunohistochemical quantitative analysis using image analyser in 25 cases of astrocytomas , grade 2 to 4 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The topographical distribution of glial fibrillary acidic protein ( GFAP ) , vimentin , neuron specific enolase ( NSE ) and neurofilament ( NF ) proteins in the developing neocortex and cerebellum of sheep fetuses of different gestational ages ( 60 149 days ) was described . ^^^ In the walls of the developing cerebral hemispheres GFAP and vimentin immunoreactive radial glial fibres were demonstrated . ^^^ In Bergmann glial fibres of the cerebellum , which are the equivalent of radial fibres in the telencephalon , both GFAP and vimentin were detectable in fetuses and adult sheep . ^^^ GFAP and vimentin in radial glial fibres were not demonstrable without pretrypsinization of tissue sections . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
One component showed large cells with eosinophilic cytoplasm , vesicular nuclei with prominent nucleoli , eosinophilic perinuclear inclusions , and immunoreactivity for glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies revealed that the cytoplasm of rhabdoid cells had conspicuous structures , resembling weakly eosinophilic homogeneously amorphous inclusion bodies , and that the strongly eosinophilic cytoplasm , seen abundantly in the gemistocytic astrocyte like cells , was a mixture of components that were positive for glial fibrillary acidic protein ( GFAP ) and vimentin and of components positive for myoglobin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To visualize astrocytes , microglia , and macrophages , antibodies against glial fibrillary acidic protein ( GFAP ) , vimentin ( VIM ) , complement receptor type 3 ( OX 42 ) , and ED 1 were used . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To resolve the depth and orientation of the tissue reaction regarding the localization of reactive astrocytes , we looked for the expression of glial proteins like glial fibrillary acidic protein ( GFAP ) , Vimentin and S 100 with a three step biotin avidin immunoperoxidase method . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At both permissive ( 33 degrees C ) and non permissive ( 38 . 5 degrees C ) temperatures , all cell lines were positive for vimentin , two demonstrated glial fibrillary acidic protein ( GFAP ) immunoreactivity , and none expressed oligodendrocyte or microglial markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have reinvestigated the origin and genesis of the radial glia of the cerebellar cortex in the hamster using three astroglial markers , vimentin , GFAP , and S 100 protein antibodies . ^^^ On embryonic day 12 ( E 12 ) , before the emergence of the external granular layer , the cerebellar anlage is traversed from the ventricle to the pial surface by a primordial radial glial scaffold which is vimentin positive , but GFAP and S 100 negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tissue specimens and culture cells from three human gliomas ( two astrocytomas and one glioblastoma ) were immunohistochemically investigated , using GFAP , S 100P , vimentin , FN and TNF antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , most of the tumor cells reacted positively to Vimentin , but negatively to S 100 protein and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of glial antigens vimentin ( VIM ) and glial fibrillary acidic protein ( GFAP ) is described in the pineal gland of cats and dogs from the first postnatal days to adulthood . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical reactions with antisera to glial fibrillary acid protein ( GFAP ) , cytokeratin , vimentin , desmin , and S 100 demonstrated vimentin positivity in five of nine patients and cytokeratin positivity in two of nine patients . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
As a first step toward addressing the possibility that glial maturation and / or the development of an astrocytic response to lesioning contribute to loss of rubrospinal plasticity , we have studied the normal development of radial glia and astrocytes in the spinal cord of the opossum by immunostaining for vimentin ( Vim ) and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunoreactivity of ependymal cells for vimentin and GFAP was normal for age , but abnormally positive for S 100 protein and cytokeratin , as in lissencephaly ; unlike lissencephaly , the rosettes in CHR disease also are reactive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The present study examined the immunocytochemical localization of various intermediate filaments ( IFs ) , 68 kDa , 160 kDa and 200 kDa neurofilament protein ( NFP ) , cytokeratin ( CK ) 1 , 8 , 10 and 19 , vimentin , and glial fibrillary acidic protein ( GFAP ) in the vestibular end organs and ganglia of normal and streptomycin treated guinea pigs . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies against type 1 4 collagen , fibronectin ( FN ) , glial fibrillary acidic protein ( GFAP ) , vimentin and the monoclonal antibody ( MAB ) against human Mller cells were used to examine the membranes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The denervation induced changes on S 100 protein , glial fibrillary acidic protein ( GFAP ) and vimentin immunoreactivity ( IR ) of the lamellar cells from cutaneous Meissner like sensory nerve formations ( SNF ) , or corpuscles , of the adult rat hind limb foot pads were studied , using combined immunohistochemical and image analysis ( optic microdensitometry ) techniques . ^^^ Lamellar cells of Meissner like corpuscles displayed S 100 protein and vimentin IR , but not GFAP IR . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The developmental expression of the glial antigens , vimentin ( VIM ) , glial fibrillary acidic protein ( GFAP ) , and S 100 protein is described in the rat pineal gland from the first postnatal day to adulthood . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Co expression of GFAP and vimentin in astrocytes proliferating in response to injury in the mouse cerebral hemisphere . ^^^ Changes in the distribution of proliferating astrocytes expressing glial fibrillary acidic protein ( GFAP ) and / or vimentin were examined in the injured cerebral hemisphere in adult mice . ^^^ The brain sections were doubly immunostained for GFAP and vimentin and subjected to autoradiography . ^^^ In that way three cell types were distinguished immunocytochemically : ( 1 ) astrocytes co expressing glial fibrillary acidic protein ( GFAP ) and vimentin , ( 2 ) astrocytes expressing only GFAP and ( 3 ) astrocyte like cells expressing vimentin . ^^^ Two hours as well as 1 day after the injury proliferation of GFAP positive astrocytes and of those co expressing GFAP and vimentin could only be seen as statistically insignificant phenomena . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have examined the injury response of astrocytes in the immature hamster brain in this study , focusing on alterations in the expression of glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Double label immunofluorescence studies of this material suggested that GFAP as well as vimentin protein levels were also increased in many astrocytes in and around the degenerating corticospinal tract 2 14 days after axotomy . ^^^ Most of the reactive astrocytes in the degenerating regions exhibited increases in GFAP and vimentin immunostaining but some vimentin negative GFAP positive reactive astrocytes were also observed , particularly in regions surrounding the actual degenerative zones . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To assess the biological characters of cells , expression of GFAP , vimentin , A2B5 and p 53 product were determined by immunohistochemistry and flow cytometry . ^^^ Both primary cultured and ENU treated cells were positive for GFAP and vimentin , but population of A2B5 positive cells was less than 5 % , thus indicating that these cells were astroglial in origin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Forty nine cases of the so called `` angioblastic meningioma ' ' , six cases of cerebellar hemangioblastoma , and four cases of soft tissue hemangiopericytoma were studied with HE , reticulin stainings , and immunohistochemical reaction with Vimentin , EMA , GFAP , S 100 , and UEA 1 antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The plasmacytoid tumors were each immunoreactive for vimentin , cytokeratin , S 100 protein , and glial fibrillary acidic protein ( GFAP ) . ^^^ One tumor also contained focal positivity for desmin and GFAP , and a single spindle cell tumor was vimentin positive only . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In astrocytes we studied the phosphorylation of the two major cytoskeletal proteins , vimentin and GFAP , which is normally stimulated by isoproterenol , and found that gp 120 partially ( 40 50 % ) prevented such stimulation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Seventy four cases of human astrocytoma were studied with ABC method for GFAP and vimentin . ^^^ Data indicated that all of the specimens in this series contained two different intermediate filament proteins : GFAP and vimentin . ^^^ There was a negative correlation between the degree of anaplasia and the number of GFAP positive cells , but no definite relationship was seen between the degree of anaplasia and the number of vimentin positive cells . ^^^ Generally , two immunostainings are used in demonstrating GFAP and vimentin : one for intense perikaryo staining and the other for cell processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The phenotypes of the cells were defined on the basis of their immunologic composition with respect to IFAP 70 / 280 kD ( 1 ) , vimentin ( 5 ) and GFAP ( G ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These lesions were examined for localization of the following antigens : cytokeratins , epithelial membrane antigen , secretory component , carcinoembryonic antigen , prealbumin , vimentin , glial fibrillary acidic protein ( GFAP ) , S 100 protein , neuron specific enolase , 68 kD neurofilament protein , chromogranin , serotonin , and lysozyme , and with Leu 7 monoclonal antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using a double immunofluorescent staining method , reactive astrocytes which express GABA immunoreactivity were also found to immunostain with either GFAP or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Adsorption with purified vimentin substantially reduced cytotoxicity , while adsorption with glial fibrillary acid protein ( GFAP ) had no cytoprotective effects . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The morphology and growth rate of cultured cells were examined and correlated with the presence of vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ The cultures from various brain matters differed in cell morphology and rate of growth but not in GFAP and vimentin staining . ^^^ Cells of primary and rapidly proliferating cultures were GFAP negative and vimentin positive . ^^^ This deceleration , as well as the successive complete growth arrest , were accompanied by an appearance of GFAP positive cells and an elevated intensity for vimentin staining . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Seventy nine cases ( 96 % ) were positive for vimentin , only five cases and two cases were focally and weakly reactive for S 100 protein and GFAP respectively , and none stained for neurofilaments . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The following immunocytochemical markers were used to characterize the different retinal cell types : antibodies against protein kinase C ( PKC ) , calcium binding protein ( CabP 28kD ) , neurofilaments ( NF ) , glia specific antibodies ( GFAP , vimentin ) , and transmitter specific antibodies ( GABA , TH ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution and the structural , ultrastructural and immunohistochemical characteristics of the astroglial cells in the spinal cord of the adult barbel ( Barbus comiza ) have been studied by means of metallic impregnations ( Golgi and gold sublimate ) , immunohistochemical ( GFAP and vimentin ) and electron microscopic techniques . ^^^ The ependymocytes were heterogeneous cells because they showed different immunohistochemical characteristics : GFAP positive , vimentin positive or non immunoreactive cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Type 3 cells are also immunoreactive for glial fibrillary acidic protein ( GFAP ) , alpha actin , S 100 protein , vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Positive immunostaining for S 100 protein , Leu 7 antigen , vimentin and glial fibrillary acidic protein ( GFAP ) supported the schwann cell nature of these tumors . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have studied the postnatal evolution of the glial cells in the rat pineal gland after its chemical pre and perinatal denervation , by the assessment of the immunocytochemical expression of three antigens characteristic of glial cells i . e . , vimentin ( VIM ) , glial fibrillary acidic protein ( GFAP ) , and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
CONCLUSIONS : The increased GFAP and vimentin immunoreactivity and the morphometric and cytological changes in rat SON astrocytes may reflect a sustained upregulation of cellular activity with age , resulting in hypertrophy of glial perikarya and cell processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocyte activation was studied at various time points by immunocytochemistry for glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This study has characterized the subventricular zone of late embryonic , postnatal , and adult mice using several neuronal markers [ TuJ 1 , nicotinamide adenine dinucleotide phosphate diaphorase ( NADPH d ) , neuron specific enolase ( NSE ) ] , glial markers [ RC 2 , vimentin , glial fibrillary acidic protein ( GFAP ) , galactocerebroside ( Gal C ) ] , ECM markers [ tenascin C ( TN C ) , chondroitin sulfate , a chondroitin sulfate proteoglycan termed dermatan sulfate dependent proteoglycan 1 ( DSD 1 PG ) ] , stem cell marker ( nestin ) , and proliferation specific marker [ bromodeoxyuridine ( BrdU ) ] . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical study of the ASC demonstrated the presence of actin ( smooth muscle and muscle specific ) , KP 1 , and vimentin ; no reactivity was seen with desmin , myoglobin , S 100 protein , or glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunohistochemical study was positive for glial fibrillary acidic protein ( GFAP ) and neuron specific enolase ( NSE ) in 1 of the 2 tumours and positive for S 100 protein and vimentin in both tumours but negative for CD 68 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Eosinophilic giant cells of tubers were positive for vimentin ( 100 % ) , GFAP ( 77 % ) and S 100 protein ( 92 % ) ; such cells were also found to a various extent to be reactive for neuron associated antigens , including neurofilament ( NF ) proteins ( 38 % ) or class 3 beta tubulin ( 77 % ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial like cells of the rat pituitary intermediate lobe change morphology and shift from vimentin to GFAP expression during development . ^^^ This study demonstrated morphological changes in glial like cells of the rat pituitary intermediate lobe during early postnatal development , and a subsequent shift in protein expression from vimentin to GFAP . ^^^ The transition from vimentin to GFAP expression and concurrent morphological changes resemble those described for radial glial during cerebral cortical development . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemically , the tumour cells expressed vimentin , S 100 protein , epithelial membrane antigen , cytokeratins , and most surprisingly , glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Developmental pattern of GFAP and vimentin gene expression in rat brain and in radial glial cultures . ^^^ In the present study we analyze the events which occur during the early stages of astrogliogenesis by examining the pattern of both GFAP and vimentin gene expression and their corresponding immunoreactive proteins during rat brain development . ^^^ The biological significance of the developmental patterns of GFAP and vimentin expression in astroglial cells . during brain development is discussed . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In these regions , 6 . 17 immunolabeling was associated with S 100 , GFAP and vimentin positive astrocytes constituting the glial scar . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical staining for glial fibrillar acidic protein ( GFAP ) and vimentin were used to evaluate astrocyte response . ^^^ After one year of treatment , the immunostaining of homologous sections showed an increase of GFAP reactivity and the presence of vimentin + cells in the upper and the lower limb , and in the hilus of the fascia dentata . ^^^ GFAP and vimentin astrocytic response extended to the adjacent cerebral cortex after 14 months . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Commercially available antibodies directed against high and low molecular weight keratins ( keratin , RCK 102 , NCL 5D3 ) , vimentin , desmin , glial fibrillary acidic protein ( GFAP ) , and neurofilament intermediate filament ( IF ) proteins were used in the avidin biotin peroxidase complex technique on formalin fixed , paraffin embedded tumor tissue samples . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The identity of the isolated cells was confirmed by immunodetection of carbonic anhydrase 2 ( CA 2 ) , cellular retinaldehyde binding protein ( CRALBP ) , glial fibrillary acidic protein ( GFAP ) , vimentin , and delta smooth muscle actin ( alpha SMA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The study of its expression in the astrocyte lineage during development and in reactive astrocytes has revealed an intricate relationship with the expression of vimentin , another intermediate filament protein widely expressed in embryonic development . these findings suggested that vimentin could be implicated in the organization of the GFAP network . ^^^ To address this question , we have examined GFAP expression and network formation in the recently generated vimentin knockout ( Vim ) mice . ^^^ We show that the GFAP network is disrupted in astrocytes that normally coexpress vimentin and GFAP , e . g . , those of the corpus callosum or the Bergmann glia of cerebellum . ^^^ The role of vimentin in this process was further suggested by transfection of Vim cultured astrocytes with a vimentin cDNA , which resulted in the normal assembly of the GFAP network . ^^^ We demonstrate that in Vim mice , reactive astrocytes that normally express both GFAP and vimentin do not exhibit GFAP immunoreactivity , whereas those that normally express GFAP only retain GFAP immunoreactivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies ( for synaptophysin , chromogranin A , GFAP , vimentin ) together with morphology and localization of neoplasm suggested diagnosis of primitive neuroectodermal tumor ( PNET ) that spread mainly in the leptomeninges and caused obliteration of subarachnoid space . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The SVG cell line is vimentin , GFAP , Thy 1 . 1 and MHC class 1 positive , and negative for neurofilament and neuron specific enolase , consistent with its glial origin . ^^^ A panel of antibodies was used to unequivocally differentiate the engrafted cells from the host parenchyma , including antibodies to : SV 40 large T antigen ( expressed in the SVG TH cells ) , human and rat MHC class 1 , vimentin , GFAP , and tyrosine hydroxylase . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The antibodies for intermediate filaments , including keratin , vimentin , desmin , GFAP and neurofilaments , have been much useful in routinely processed immunohistochemical study for identifying characteristics of tumor cells or making the definite diagnosis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumour diagnoses were immunohistochemically proved by various antibodies to cytokeratins , vimentin , GFAP , NSE , von Willebrand factor , CD 3 , CD45RA , S 100 , thyroglobulin as well as histochemically with argyrophilic , Fontana Masson and heterochromatic reactions . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
After a variable time delay , immunocytochemistry of the resulting gliosis was employed to detect astrocytes with glial fibrillary acidic protein ( GFAP ) , Mller cells with vimentin , and CSPG with CS 56 antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Differential immunohistochemical diagnosis of tumors of the human central nervous system using monoclonal antibodies ] . 67 brain tumors were studied using monoclonal antibodies against glial fibrillary acid protein ( GFAP ) , neurofilaments , proteoglycan , laminin and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
By comparison , the ancestral sequence for GFAP appeared after that for vimentin . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Repeated seizures increase GFAP and vimentin in the hippocampus . ^^^ Reactive gliosis , detected by immunohistochemistry for the presence of GFAP and vimentin , was present 2 7 days after the repeated seizures . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The presence and the colocalization of the three intermediate filament proteins , glial fibrillary acidic protein ( GFAP ) and the marker of mesenchymal liver cells , vimentin , were studied by an immunofluorescence double labeling technique in cultures of isolated rat perisinusoidal stellate cells ( PSC ) and hepatocytes , in cocultures of isolated PSC and hepatocytes as well as in cryostat sections of rat liver . ^^^ GFAP and vimentin immunoreactivities were localized in cultured PSC which were identified by the presence of the cellular marker desmin , another intermediate filament protein , or the stellate morphology to be seen after staining for one of three intermediate filament proteins . ^^^ Both GFAP and vimentin were strongly expressed in the perinuclear region and the cell processes of cultured PSC . ^^^ Staining for GFAP highly coincided with that for vimentin or desmin in cultured PSC and with that for vimentin in the liver sections . ^^^ Almost all of the vimentin positive cells in hepatocyte culture were also expressing GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Gliosis developed in parallel with the onset of postnatal transgene expression and led to a dramatic increase in the number of astrocytes positive for GFAP , vimentin , and possibly tau . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Monoclonal antibodies for cytokeratins ( Cks ) , vimentin , neurofilament protein ( NF ) , and glial fibrillary acidic protein ( GFAP ) were used and visualized with an indirect ABC method . ^^^ Vimentin and NF were stained positively , GFAP negatively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical stains using antibodies to glial fibrillary acidic protein ( GFAP ) , vimentin and S 100 were also performed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to elucidate the immunohistochemical features of hydrocephalic ependyma , immunohistochemical examination was undertaken in 11 normal , postmortem brains ( age range , 11 weeks ' postconception to 6 months after birth ) and 12 hydrocephalic brains ( three cases each of congenital aqueductal stenosis , Dandy Walker malformation , Arnold Chiari type 2 malformation and posthemorrhagic hydrocephalus ) by using antisera to nestin , vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ Vimentin immunoreactivity was principally detected in immature ependymal cells and their basal fibers after the period of neuronal migration , then partly replaced by GFAP reactivity during late gestation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
By in situ hybridization , mRNAs for GFAP , vimentin , and clusterin were upregulated when compared to the control hemisphere ( which received heat inactivated CNTF ) . ^^^ By immunocytochemistry , GFAP , vimentin , glutathione S transferase mu , S 100 , and OX 42 were elevated by 48 h . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used antibodies against glial fibrillary acidic protein ( GFAP ) , vimentin , the S1OO protein ( S1OOP ) , lysozyme , and ED 1 as markers for astroglial cells and microglia / macrophages at the light and electron microscopic level during the 3 weeks following the crush . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
They upregulate astrocytic specific intermediate filament glial fibrillary acidic protein ( GFAP ) and show a positive signal for the intermediate filament vimentin , a protein primarily found in developing astrocytes . ^^^ The astrocytic intermediate filaments GFAP and vimentin were upregulated in a time dependent manner 2 days after injury . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes in ventromedial temporal , frontal , and calcarine cortices were immunohistochemically identified with monoclonal antibodies directed at glial fibrillary acidic protein ( GFAP ) and vimentin , and NFTs were labeled with an anti tau antibody specific for paired helical filaments . ^^^ There were no increases in GFAP or vimentin immunoreactive astrocyte counts , GFAP optical density , or NFT counts for the schizophrenic group as a whole compared to the non neuropsychiatric group , while both groups differed from AD . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the teratoma the neuroectodermal part was strongly HNK 1 positive and contained GFAP and vimentin positive astrocytes and some NSE positive neuron like cells . ^^^ The omental nodules consisted of astrocytes , which expressed GFAP , vimentin and desmin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cultures were characterized using antibodies against neuron specific enolase ( NSE ) , tyrosine hydroxylase ( TH ) , vimentin , glial fibrillary acidic protein ( GFAP ) , and the antigen A2B5 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
As analyzed by indirect immunofluorescence , the A2B5 surface marker is absent , they express galactocerebroside and myelin specific proteins , such as myelin basic protein ( MBP ) , myelin associated glycoprotein ( MAG ) , proteolipidprotein ( PLP ) , and Wolfgram protein ( WP ) , but do not exhibit astrocytic properties , such as the expression of vimentin or the glial fibrillary acidic protein ( GFAP ) . ^^^ Immunoblot analysis further confirmed the presence of MAG , WP and MBP immunoreactivity , and the absence of vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies against alpha internexin , the three neurofilament subunits ( NF L , NF M , NF H ) , vimentin , and glial fibrillary acidic protein ( GFAP ) were used to determine the relative expression of these proteins at different post natal stages of mouse retinal development . alpha Internexin is widely distributed in the process of amacrine cells , horizontal cells and retinal ganglion cells before post natal day 5 ( P 5 ) . ^^^ Vimentin is present in all glial cells ( astrocytes and Mller cells ) and some horizontal cell processes during development , while GFAP is found only in astrocyte processes of the mature retina . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
BMSC expressed fibronectin , alpha actin , beta tubulin , vimentin and vinculin while cytokeratinpan , GFAP , neurofilament , desmin and laminin were not expressed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry was performed using antibodies against HMB 45 , S 100 protein , NSE , cytokeratins , vimentin , desmin , GFAP , the HNK 1 carbohydrate epitope and alpha smooth muscle actin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunohistochemical features were similar for the benign and malignant tumors , with positivity for S 100 protein , vimentin , cytokeratins , and CAM 5 . 2 , and were negative for GFAP , muscle specific actin , CEA , and desmin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
One cell subset expressed cytokeratin 14 , S 100 protein , GFAP , vimentin and smooth muscle actin immunoreactivity ; this antigenic profile is consistent with myoepithelial differentiation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The diagnosis was confirmed by immunoperoxidase studies , that showed positivity for vimentin , whereas the staining for cytokeratin AE 1 , glial fibrillar acid protein ( GFAP ) and collagen 4 was negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Spinal cords have been examined at the cervical , thoracic and lumbar levels by histological methods and immunohistochemistry for GFAP , Vimentin , Tau protein , Neurofilaments , PCNA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies showed focal reactivity for glial fibrillary acidic protein ( GFAP ) in the retina adjacent to the schisis cavity in all five globes , focal reactivity for S 100 protein in four retinas , rare focal staining for vimentin and neurofilaments in two retinas each and no reactivity for type 1 keratin or actin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Primary cultures derived from the telencephalic region of 3 day old chick embryo ( stage 19 ) exhibited intense staining for vimentin ( Vim ; indicative of immature glial phenotypes ) throughout the 17 day culture period , transitioning to Vim positive / glial fibrillary acidic protein ( GFAP ) positive astroblasts after a single cell passage at 13 days in vitro ( DIV ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At these times the majority of the cells identified in the median eminence cultures exhibited immunostaining patterns of tanycytes , as detected in the mediobasal hypothalamus of 10 day old and adult rats , i . e . , they were immunoreactive to vimentin ( VIM ) , to DARPP 32 ( a dopamine and adenosine 3 ' : 5 ' monophosphate regulated phosphoprotein ) , and to a lesser extent to glial fibrillary acidic protein ( GFAP ) antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP and vimentin stained positive in all cases . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The gold colloid immuno cytochemical method was used to stain GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We investigated whether unilateral removal of the labyrinthine and cochlear receptors induces a macroglial reaction in rat vestibular and cochlear nuclei using vimentin and glial fibrillary acidic protein ( GFAP ) immunochemical markers . ^^^ Furthermore , double labelling experiments demonstrated that these vimentin positive cells were also GFAP positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To further understand the role of the subplate in human brain development , we have studied the immunohistochemical expression of certain neuronal ( GAP 43 , MAP 2 , parvalbumin ) and astroglial ( vimentin , GFAP ) markers in the developing visual cortex from gestational ages of 14 weeks to 9 months post term . ^^^ Vimentin and GFAP did not stain differentiated neuronal cells . ^^^ Vimentin immunoreactivity appeared early in neuroepithelial and radial glial cells , decreasing after 35 weeks , with a concomitant increase in GFAP immunoreactivity in radial glial and maturing astrocytic cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Smooth Muscle Actin , Cytokeratin 14 , GFAP , S 100 Protein and Vimentin immunoreactivity have clearly demonstrated that cells with myoepithelial immunophenotype are one of the major cell components in breast adenomas . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using a double fluorescence immunostaining , we characterized inoculated CNS cells , with a TH antiserum and one of the following antibodies : microtubule associated protein ( MAP 2 ) and GABA for neuronal cells , vimentin ( Vim ) for glial cells and glial fibrillary acidic protein ( GFAP ) for astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have investigated the effects of ethanol on the early stages of astrogliogenesis by analyzing the developmental pattern of vimentin and glial fibrillary acidic protein ( GFAP ) immunoreactivity and their mRNA levels during embryonic / fetal brain development and in radial glia in primary culture . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : Sections and wholemounts of retinas from fetal day ( Fd ) 65 to adult animals were analyzed immunohistochemically to detect glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Occasional spindle shaped cells containing GFAP or vimentin were identified as immature glia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor cells in both cases expressed cytokeratins ( CK 22 and CAM 5 . 2 ) but did not express glial fibrillary acidic protein ( GFAP ) , vimentin , epithelial membrane antigen , and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This was followed by a local hypertrophy of the OX 42 immunoreactive resident tract microglial cells and an increase in both GFAP and vimentin immunoreactivity of the tract astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : All tumors were examined using antibodies against Ulex europaeus agglutinin 1 ( UEA ) , glial fibrillary acidic protein ( GFAP ) , vimentin ( VM ) , epithelial membrane antigen ( EMA ) , desmin , collagen 4 , alpha 1 antitrypsin ( alpha 1 AT ) and smooth muscle actin ( SMA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The regional distribution and temporal appearance of astrocytes expressing glial fibrillary acidic protein ( GFAP ) , S 100 protein , and vimentin were determined in a nonpenetrating lateral fluid percussion ( LFP ) brain injury model . ^^^ Quantitative assessment of GFAP , S 100 , and vimentin polypeptides confirmed the immunohistochemical evaluation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies to prealbumin , glial fibrillary acidic protein ( GFAP ) , vimentin , keratin , and carcinoembryonic antigen ( CEA ) were used to determine the immunohistochemical features of interest . ^^^ Expression of GFAP , vimentin and keratin varied from one CPP case to another . ^^^ Both CPCs expressed CEA , but not prealbumin , GFAP and keratin ; one of them was vimentin positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astroglial elements ( radial glia and astrocytes ) were studied by glial fibrillary acidic protein ( GFAP ) and vimentin immunohistochemistry on post transplantation ( PT ) days 7 , 11 , 14 , 17 , and 21 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemistry of paraffin embedded tissues of the neoplasms revealed immunoreactivity with antibodies against S 100 protein , glial fibrillary acidic protein ( GFAP ) , vimentin , neuron specific enolase ( NSE ) and bcl 2 . ^^^ S 100 , GFAP and vimentin were positive only for glial elements ( reactive astrocytes ) and , NSE and bcl 2 for almost all tumor cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Confocal microscopic image analysis of human and rat retinae showed colocalization of 10 FDH primarily with Muller cell protein markers [ glial fibrillary acidic protein ( GFAP ) , vimentin , and carbonic anhydrase ] . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Following HOTC exposure to N methyl D aspartate ( NMDA ) , dose dependent changes occurred in the optical density ( OD ) values for the astrocytic immunohistochemical [ immunostaining ( IS ) ] markers glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ It also significantly increased GFAP IS and vimentin IS OD values in these regions . ^^^ Increased GFAP IS and vimentin IS OD values were also seen in CA 3 , a hippocampal region that displayed no cell death . ^^^ Light microscopic examination revealed hypertrophied GFAP and vimentin IS cells , characteristic of reactive astrocytes . ^^^ In contrast , exposure of HOTCs to a noninjurious level of NMDA ( 1 microM ) caused only minor changes in GFAP IS and vimentin IS OD values but a significantly reduced cellular proliferation in all HOTC regions . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP . ^^^ Vimentin and glial fibrillary acidic protein ( GFAP ) are intermediate filament proteins expressed in the cytoplasm of various types of cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to address this question , we have used double labelling immunofluorescence techniques for detecting smooth muscle alpha actin ( SMAA ) and the intermediate filament proteins , GFAP , desmin and vimentin , taking advantage of the fact that PSC present in primary cultures of rat hepatocytes proliferate and transform . ^^^ GFAP and vimentin were expressed in PSC throughout cultivation , while desmin which stained only about half of the PSC in early cultures was expressed in all GFAP positive cells later on . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the intact brain and spinal cord of adult rats , Mab22C11 immunostaining was associated with ( 1 ) numerous neuron like structures and ( 2 ) glial structures immunostained for glial fibrillary acidic protein ( GFAP ) and / or vimentin , including tanycytes mostly located in the mediobasal hypothalamus , fibrous astrocytes located in the white matter and ependymocytes bordering the ventricles . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A subpopulation of betaA treated astrocytes showed an increase in glial fibrillary acidic protein ( GFAP ) and Vimentin ( Vim ) immunostaining while other reactive astrocyte markers such as S100beta , MAP 2 , and ApoE remained unaltered or undetectable . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : Light microscopic immunocytochemistry using antibodies to VEGF , bFGF , vimentin , glial fibrillary acidic protein ( GFAP ) , and factor 8 on frozen sections from eyes of patients with diabetes without proliferative retinopathy , eyes of patients without diabetes and without known ocular disease , and eyes with disciform age related macular degeneration ( ARMD ) . ^^^ RESULTS : Based on morphology and on GFAP and vimentin immunopositivity , retinas from all subjects with diabetes immunostained strongly to VEGF in elongated processes that appeared to be Mller cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
FS cells were identified according to the reactivity to S 100 , GFAP and vimentin . ^^^ GFAP stained only a percentage of FS cells , while vimentin recognized in addition to stellate cells endothelia , perivascular and infiltrating macrophages . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The identity of the isolated cells was confirmed by immunodetection of carbonic anhydrase 2 ( CA 2 ) , cellular retinaldehyde binding protein ( CRALBP ) , glial fibrillary acidic protein ( GFAP ) , vimentin , and alpha smooth muscle actin ( alpha SMA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Standardized postembedding immunoelectron microscopy was performed to demonstrate glial fibrillary acidic protein ( GFAP ) and vimentin in individual intermediate filaments to determine the diagnostic value of demonstrating ultrastructural and immunophenotypic characteristics of intermediate filaments in routine brain biopsy specimens . ^^^ Dual expression of GFAP and vimentin was observed in the astroblastoma and astrocytes of Alexander ' s disease . ^^^ The antigen availability for vimentin , however , was too low to allow reliable assessment of the GFAP : vimentin ratio in individual intermediate filaments and / or filament bundles . ^^^ Nevertheless , application of the postembedding approach to routinely fixed biopsy specimens indicated an association of different entities with the exclusive presence of GFAP and / or vimentin in individual intermediate filaments , thus emphasizing the diagnostic value of intermediate filament typing for pathological characterization . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This staining pattern was different from the continuous and linear gold labeling of GFAP and vimentin . ^^^ MAb 8E1 did not immunoblot purified human brain GFAP nor bovine lens vimentin , and so was not neutralized by preabsorption with these molecules . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We report that CNTF increases glial fibrillary acidic protein ( GFAP ) , while having no appreciable effect on the levels of other intermediate filaments including vimentin and neurofilament . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the cells were slightly positive for NSE and negative for S 100 , GFAP , neurofilaments , squamous cell cytokeratin 1 , vimentin , desmin and leukocyte common antigen . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In contrast to the proliferating cultures , the bFGF treated cultures were strongly immunoreactive for GFAP , only mildly immunoreactive for nestin and vimentin , and negative for A2B5 , CNPase , NF , and NSE . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor was reactive to vimentin and CD 34 but was negative for glial fibrillary acid protein ( GFAP ) , S 100 , smooth muscle actin , epithelial membrane antigen , HMB 45 , myelin basic protein , and keratin ; ultrastructural examination showed fairly undifferentiated cells within a collagenous matrix , few tight junctions , and sparse extravascular basement membrane . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Frozen sections were immunohistochemically stained with monoclonal antibodies to three human CD 44 clones ( BU 52 , F 10 44 2 , and DF 1485 ) and to vimentin , and antiserum to glial fibrillary acidic protein ( GFAP ) . ^^^ RESULTS : Positive immunostaining for CD 44 was first detected at 21 weeks of gestation in the longitudinal fibers that extended from the inner to the outer limiting membrane and around capillary vessels with the simultaneous expression of vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These results raise the possibility that PKN may regulate filament structures of vimentin and GFAP by domain specific phosphorylation . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The molecular characterization of glial lineage cells in two urodele species , Ambystoma mexicanum and Triturus carnifex , has been investigated immunocytochemically with antibodies directed against intermediate filament proteins , glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Ambystoma astroglia shows clear GFAP immunopositivity and vimentin immunonegativity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumors basically showed the same immunostaining pattern as MHH MED 1 cells in vitro expressing neuron specific enolase ( NSE ) and vimentin , but no neurofilaments ( NFs ) , synapthophysin , or glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Pronounced immunoreactivity for ubiquitin and alpha 1 antichymotrypsin could be found in all investigated tumours , while GFAP , neuron specific enolase , von Willebrand factor , vimentin , S 100 protein , alpha 1 antitrypsin , actin , and the neurofilaments 68 kDa and 160 kDa showed mostly weak positivity in some cases . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These cells were arranged as chains parallel to the walls of the ventricle and were polysialylated neural adhesion cell molecule ( PSA NCAM ) , TuJ 1 ( beta tubulin ) , and nestin positive but GFAP and vimentin negative . ^^^ Chains of Type A cells were ensheathed by two ultrastructurally distinct astrocytes ( Type B 1 and B 2 ) that were GFAP , vimentin , and nestin positive but PSA NCAM and TuJ 1 negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used antisera to vimentin ( 5 ) , desmin ( D ) , epithelial membrane antigen ( EMA ) , prostate specific antigen ( PSA ) , pancytokeratin , carcinoembryonic antigen ( CEA ) , glial fibrillary acidic protein ( GFAP ) and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These two different compartments showed different characteristics as regards cellular differentiation , proliferative activity , and expression of oncogene and tumour suppressor oncogene proteins , as revealed by using markers for muscle actin , keratin , vimentin , S 100 protein , GFAP , Ki 67 , p 53 , and c erbB 2 proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Neither 4F3 nor 3F8 recognized GFAP or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
R 28 cells were analyzed both immunocytochemically and by western immunoblot for GFAP , S 100 , and vimentin . ^^^ RESULTS : GFAP , S 100 and vimentin immunoreactive proteins were detected in R 28 cells . ^^^ CONCLUSIONS : There is a very strong glial component to the R 28 retinal precursor cell line , as evidenced by the expression of proteins immunoreactive to GFAP , S 100 and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using immunocytochemical techniques , these cells were stained with monoclonal antibodies specific for Mller cells , glial fibrillary acidic protein ( GFAP ) , vimentin , glutamine synthetase ( GS ) and keratin . ^^^ RESULTS : The dissociated and cultured cells expressed vimentin and GS , but not GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The large cells within these nodular clusters were variably immunoreactive for glial fibrillary acidic protein ( GFAP ) and vimentin and negative for synaptophysin and neurofilament . ^^^ Subependymal radial glia expressed both vimentin and GFAP , but subpial radial glia either did not express these markers ( in contrast to an age matched control ) or were absent . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemical analysis showed that primary cultures were composed of colonies of oligoclonal cells that expressed the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) , vimentin , as well as glutamine synthetase ( GS ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using confocal microscopy we examined the expression of transitin , vimentin and glial fibrillary acidic protein ( GFAP ) in cultured astrocytes , and show that transitin co distributes with these other glial intermediate filament proteins . ^^^ The expression of transitin , vimentin and GFAP was also compared in embryonic chick spinal cord and brain radial glia , with these studies showing that these intermediate filament proteins display distinct expression patterns during CNS development . ^^^ Of particular note is the absence of vimentin and GFAP in spinal cord midline radial glia that express transitin protein , and a transient expression of transitin in brain midline radial glia that continue to express vimentin . ^^^ Our studies presented here therefore indicate that transitin , a novel radial glial intermediate filament protein , may have functions that are unrelated to GFAP or vimentin during CNS development , since transitin is localized to the processes of midline radial glia and is transiently expressed during chick CNS development . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies were positive for GFAP , vimentin , S 100 , CK , EMA , and SMA , but negative for AFP , HCG , PLAP , and CEA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To characterize the giant cells in tuberous sclerosis , we examined immunoreactivity for nestin , vimentin , microtubule associated protein 1B ( MAP1B ) , MAP 2 , neurofilament , and glial fibrillary acidic protein ( GFAP ) in cortical tubers detected in brain specimens from 6 patients with tuberous sclerosis who had undergone surgical resection for treatment of intractable epilepsy . ^^^ These neuron like giant cells demonstrated consistently strong immunoreactivity for neurofilament and MAP1B and occasional immunopositivity for nestin and vimentin and were rarely positive for GFAP . `` Indeterminate giant cells , ' ' characterized by abundant cytoplasm , an absence of Nissl substance , and one or more eccentric nuclei , demonstrated consistent immunoreactivity for nestin , vimentin , and MAP1B and were rarely positive for neurofilament , but more than half displayed immunoreactivity for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Retinas from rabbits on embryonic day ( E ) 26 to postnatal day ( P ) 50 and from adults were subjected to immunohistochemistry with antibodies to glial fibrillary acidic protein ( GFAP ) , vimentin , glycolipid O 4 , myelin basic protein ( MBP ) , galactocerebroside ( Gal C ) , 4D6 , and Griffonia simplicifolia isolectin , markers chosen for their specificity for astrocytes , cells of the oligodendrocyte lineage , Mller cells , and microglia . ^^^ Their numbers and distribution increased markedly between E 26 and E 30 , but between E 29 and P 3 , vimentin expression was replaced by GFAP expression . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Alterations observed from PN 21 to PN 140 were assessed by antibodies to the 70kDa heat shock proteins ( hsp ) , glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) using immunohistochemistry , transmission electron microscopy ( TEM ) , and computer assisted image analysis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Knockout of the gene coding for GFAP leads to a loss of cerebral white matter , and knockout of the gene coding for vimentin causes degeneration of the cerebellar Purkinje cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Staining with the astrocyte specific markers GFAP , S 100 and vimentin yielded equivocal results , but did not support a major role for astrocytes in lesion formation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This rearrangement is accompanied by reorganization of GFAP , vimentin and actin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Microscopic findings of the surrounding area included numerous spindle shaped cells which were positive for GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The intermediate filament proteins , glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) , were detectable at birth in radially oriented , fibrous cells , but GFAP IR was restricted to the ventral half of the cord . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocyte reactivity was evaluated using immunohistochemical methods with antibodies against : protein S 100 , GFAP , Vimentin and recombinant parts of the Ki 67 molecule ( MIB 1 antibody ) during 32 days after the disease onset . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Here we studied the suppressive effects of hirudin on the inflammation , vimentin positive astrocytes , and glial fibrillary acidic protein ( GFAP ) positive astrocytes using rat cerebral ablation models . ^^^ Antibodies for monocytes / macrophages , GFAP , and vimentin were used to assess the infiltration of inflammatory cells and reaction of astrocytes . ^^^ The number of the inflammatory cells , vimentin positive astrocytes , and GFAP positive astrocytes were quantitatively analyzed . ^^^ Hirudin suppressed the infiltration of inflammatory cells and the increase in vimentin positive astrocytes , but had no effects on the increase in GFAP positive astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , almost all the tumor cells were positive for epithelial membrane antigen and vimentin , and unexpectedly , glial fibrillary acidic protein ( GFAP ) . ^^^ A few cases of meningioma with triple expression of GFAP , vimentin and cytokeratin have been reported previously . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have examined the regeneration of corticospinal tract fibers and expression of various extracellular matrix ( ECM ) molecules and intermediate filaments [ vimentin and glial fibrillary acidic protein ( GFAP ) ] after dorsal hemisection of the spinal cord of adult GFAP null and wild type littermate control mice . ^^^ In the uninjured spinal cord ( 1 ) GFAP was expressed in the wild type but not the mutant mice , while vimentin was expressed in astrocytes in the white matter of both types of mice ; ( 2 ) laminin and fibronectin immunoreactivity was localized to blood vessels and meninges ; ( 3 ) tenascin and chondroitin sulfate proteoglycan ( CSPG ) labeling was detected in astrocytes and the nodes of Ranvier in the white matter ; and ( 4 ) in addition , CSPG labeling which was generally less intense in the gray matter of mutant mice . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The blunt GFAP / astrocyte processes possessed intermediate filaments consisting mainly of vimentin , though to a lesser degree than in the wild type . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemical analysis shows that the IPNN 8 cell line is weakly positive for NFP and S 100 protein and negative for both NSE and CD 44 but positive for A2B5 and GFAP , indicating glial differentiation ; whereas , the IPNT H cell line is positive for GFAP , glutamine synthetase , A2B5 and CD 44 but only weakly positive for vimentin . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The colocalization of desmin and glial fibrillary acidic protein ( GFAP ) in astrocytes was inferred from previous studies demonstrating a unique antigenic composition comprising GFAP , desmin and vimentin in perisinusoidal stellate cells ( PSC ) of liver which share several features with astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cells were positive for vimentin , desmin , and glial fibrillary acidic protein ( GFAP ) , but negative for alpha smooth muscle actin ( alpha SMA ) . ^^^ Sorted and cultured cells were immunophenotyped for the presence of collagen types 1 , 3 , and 4 , laminin , and the cytoskeletal proteins , alpha SMA , desmin , vimentin , and GFAP . ^^^ Desmin was present in 82 % + / 1 % , vimentin in 96 % + / 2 . 5 % , and GFAP in 91 % + / 4 . 5 % of cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The direct or indirect association of calcineurin with GFAP was suggested by observations showing that FK 506 , a specific inhibitor of calcineurin , increased the phosphorylation state of GFAP in immature slices and of GFAP and vimentin in astrocyte cultures . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The cells were labeled by ( 1 ) bulk filling with the fluorescent dye , Procion yellow , and by ( 2 ) immunocytochemical demonstration of vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In Rosenthal fibers ( RF ) , astrocytic inclusions that accumulate in Alexander ' s Disease , alpha B crystallin is found with hsp 27 and skeins of intermediate filaments ( IF ) of the GFAP and vimentin types . ^^^ Cytoskeletal preparations contained alpha B crystallin , and a filamentous pattern in which alpha B crystallin co localized with GFAP and vimentin by double label immunofluorescence . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both immunoprofile which included reactivity for vimentin , low molecular weight cytokeratins , S 100 protein , GFAP ( focally ) , alfa smooth actin and muscle specific actin , and ultrastructural features including evidence of intermediate filaments ( non bundling filaments , tonofilaments ) , desmosomes , and thin filaments of actin type sustain a myoepithelial differentiation for hyaline cells of this tumor and site . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin , glial fibrillary acidic protein ( GFAP ) , and S 100 , molecules considered to be markers of myoepithelial cells by many AA . , were not found . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes in culture , similar to reactive astrocytes in vivo , express three intermediate filament proteins : GFAP , vimentin , and nestin . ^^^ GFAP / astrocytes were found to produce both nestin and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
There were no significant differences in GFAP , vimentin and HSP 70 staining between the mixed tumours , although the pure F 98 and A15A5 tumours were , unlike the pure C 6 gliomas , S 100 negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The adult human ependyma expresses no intermediate filament proteins or secretory proteins ; the fetal ependyma shows strong immunocytochemical ( ICC ) expression of vimentin , glial fibrillary acidic protein ( GFAP ) , cytokeratins ( CKs ) of high molecular weight , glycoproteins , and S 100beta protein . ^^^ GFAP is not expressed in the floor plate ependyma at any stage of development , unlike vimentin and CK . ^^^ The choroid plexus epithelium is a specialized ependyma , with an ICC profile that differs from the surface ependyma : vimentin , CK , and S 100beta protein continue to be expressed throughout fetal and adult life , but GFAP is not expressed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The qualitative expression of cytoskeletal proteins remained unchanged 10 days following irradiation compared to controls , i . e . cytokeratin was observed but not vimentin , desmin or GFAP ( glial fibrillary acidic proteins ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In dorsal lesions , catecholaminergic and serotonergic transected fibers were found to abut onto the scar formed along the surgical cut and composed of closely inderdigitating astrocyte processes strongly immunoreactive for both glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor cells revealed in all cases variegated immunoreactivity for glial fibrillary acidic protein ( GFAP ) , S 100 protein , vimentin ( VIM ) and neuron specific enolase ( NSE ) , with stronger expression of VIM than GFAP in 2 cases . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , tumor cells were strongly reactive for GFAP and vimentin , but negative or only weakly staining for EMA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor biopsy specimens were immunohistochemically analyzed for Ki 67 ( MIB 1 ) , vimentin , glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) and synaptophysin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunofluorescence staining was performed for glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To these ends , we used antibodies to glial fibrillary acidic protein ( GFAP ) and to vimentin ( to identify astrocytes and to radial glia ) and Griffonia simplicifolia ( lectin ; to identify microglia and blood vessels ) . ^^^ First , the internal capsule is never rich with radial glial fibres ( vimentin and GFAP immunoreactive ) during development ( except at P 0 ) , whereas the thalamus has many radial fibres from very early development ( E 15 E17 ) . ^^^ Second , astrocytes ( vimentin and GFAP immunoreactive ) first become apparent in the internal capsule ( E 20 P0 ) well before they do in the thalamus ( P 15 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The type 3 intermediate filament proteins ( IFPs ) : vimentin , desmin and glial fibrillary acidic protein ( GFAP ) were studied in human glioma tissue extracts , in sera from glioma patients and in low passage glioma cell lines prepared from primary cultures of freshly dissected tumors . ^^^ Radioimmunoassay ( RIA ) studies , using anti GFAP , anti desmin and anti vimentin mAbs , showed high levels of these proteins in glioma extracts . ^^^ Binding studies with these antibodies using RIAs and western immunoblotting , showed that while anti GFAF mAbs were specific to GFAP , anti desmin mAb cross reacted completely with GFAP , anti vimentin mAb cross reacted substantially with desmin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
For immunohistochemical studies deparaffinized tissue sections were incubated with antibodies against vimentin , desmin , muscle specific actin , S 100 , CD 34 , GFAP , NSE and keratin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical examination revealed that , although vimentin and NSE were positive , GFAP , synaptophysin and S 100 were negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Brain samples were processed for glial fibrillary acidic protein ( GFAP ) , vimentin , MAP 2 and SMI 311 immunocytochemistry , using different procedures . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
For those compounds which were toxic , changes in glial fibrillary acidic protein ( GFAP ) levels were measured in the primary and C 6 cultures , and changes in vimentin and S 100 measured in the C 6 cells . ^^^ The GFAP , vimentin and S 100 levels showed similar EC 50 values for the toxicants , but were not as sensitive as the NR assay . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Indirect immunofluorescence localization of vimentin , glial fibrillary acidic protein ( GFAP ) , glutamine synthetase ( GS ) , beta amyloid precursor protein ( beta APP ) , and ( alpha smooth muscle actin ( alpha SMA ) was carried out on Mller cells that were freshly dissociated , on those that had been in culture 2 and 6 days , and on confluent primary cultures and late passage cultures . ^^^ RESULTS : The expression of vimentin , GFAP , GS , and beta APP was present 2 days after dissociation and was retained through 6 days in culture , at which time alpha SMA began to be expressed in a small number of cells . ^^^ Although there was loss of expression of the intermediate filament proteins GFAP and vimentin , the expression of beta APP was maintained , whereas alpha SMA was increased and appeared to be a major cytoskeletal protein . ^^^ CONCLUSIONS : Dissociated Mller cells that were maintained in culture underwent phenotypic changes that included a large , amorphous appearance ; the loss of detectable vimentin , GFAP , and GS expression ; the persistent presence of beta APP ; and the de novo appearance of alpha SMA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At various time points , Western blot analyses were performed to study the change in GFAP and vimentin ( markers for astrogliosis ) levels . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In 10 / 17 grade 2 and 3 eyes , spindle shaped cells had vimentin , 7 / 10 had actin , 5 / 17 had GFAP , 4 / 17 had S 100 protein immunoreactivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes from the 6 OHDA lesioned side contained elevated levels of glial fibrillary acidic protein ( GFAP ) and GFAP mRNA and were intensely immunoreactive for GFAP , vimentin , and two epitopes that in vivo are found only on reactive astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In double immunolabelling analysis of adult rat kidney sections using antiserum against GFAP and monoclonal antibody ( mAb ) against vimentin or desmin , the presence of immunoreactivity for GFAP could be observed in the glomerulus of the kidney and vascular cells situated in the peritubular space which expressed vimentin and desmin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have used immunohistochemistry for calcium binding protein Calbindin D28k , astroglial proteins vimentin and glial fibrillary acidic protein ( GFAP ) , and the ultrastructural differentiation of the cerebral cortex and hippocampus to measure putative differences . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes in the Tg73 . 2 cultures appear odd shaped and enlarged , express increased levels of GFAP ( both human and mouse ) , and express alphaB crystallin protein , Hsp 27 protein , and vimentin protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the intracranial MPNST was strongly positive for S 100 protein and vimentin , and in places weakly positive for glial fibrillary acid protein ( GFAP ) . ^^^ The choroid plexus papilloma was strongly positive for epithelial membrane antigen ( EMA ) , keratin , S 100 protein and vimentin , and positive for GFAP in places . ^^^ The anaplastic ( malignant ) astrocytoma was strongly positive for vimentin , S 100 protein and keratin in the cytoplasm and fibrous processes , and weakly positive for GFAP and EMA in places . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The morphology , organization and expression of proliferating cell nuclear antigen ( PCNA ) and the cytoskeletal proteins vimentin and GFAP in immature Bergmann glial cells were studied after a developmental injury induced by a single dose of the cytotoxic agent methylazoxymethanol ( MAM ) administered on postnatal day 5 . ^^^ Vimentin and GFAP immunocytochemistry revealed a reinforcement of the Bergmann glial palisade with overexpression of both proteins and thicker immunoreactive glial processes in MAM treated rats . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The battery of antibodies included LCA , CD 3 , OPD 4 , CD 34 , UCHL 1 , L 26 , KP 1 , MAC 387 , Factor XIIIa , Leu 7 , S 100 protein , HMB 45 , GFAP , synaptophysin , neurofilament protein , ubiquitin , vimentin , type 4 collagen , laminin , fibronectin , Factor 8 related antigen , Desmin M , smooth muscle actin , cytokeratin 7 , cytokeratin 20 , AEI / AE3 , CAM 5 . 2 , EMA , GCDFP , Ki 67 , and PCNA . ^^^ Immunohistochemical stains for CD 3 , Leu 7 , S 100 protein , type 4 collagen , laminin , Factor 8 related antigen , GFAP , synaptophysin , neurofilament protein , ubiquitin , smooth muscle actin , vimentin , Desmin M , cytokeratin 7 , cytokeratin 20 , AE1 / AE3 , CAM 5 . 2 , Ki 67 and PCNA showed consistent cross reactivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
While in mammalian brain the Bergmann glia contains glial fibrillary acidic protein ( GFAP ) , in the avian Bergmann glia , the cytoskeletal protein is vimentin , which is characteristic for immature astroglia in mammals . ^^^ Free floating sections cut with a vibration microtome were processed for immunohistochemistry against GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Staining for the glial markers GFAP and vimentin was increased near the microsulcus . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The present study describes the distribution of glial fibrillary acidic protein ( GFAP ) and vimentin immunopositive structures in the brain of the domestic chicken ( Gallus domesticus ) from hatching to maturity . ^^^ In the nucleus laminaris we observed a characteristic palisade of non ependymal glia , reactive to GFAP but not to vimentin , which almost completely disappears by adulthood . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The elimination of GFAP expression in as U 251 cells was accompanied by a marked increase in vimentin , actin microfilaments and CD 44 levels . ^^^ Gold labeling density counts of cytoskeletal and cell surface elements demonstrated that the differences between GFAP , actin , CD 44 and vimentin levels in the different astrocytoma cell lines were statistically significant ( p < 0 . 05 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Most cells in the cultures co expressed GFAP and one of the two vimentin epitopes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Altered taurine release following hypotonic stress in astrocytes from mice deficient for GFAP and vimentin . ^^^ Astrocytes from wild type mice were compared with cells from mice deficient for either glial fibrillary acidic protein ( GFAP / ) or vimentin ( vimentin / ) and with astrocytes from mice deficient for both proteins ( GFAP / vim / ) . ^^^ Whereas GFAP / and vimentin / cultured or reactive astrocytes retain intermediate filaments , the GFAP / vim / astrocytes are completely devoid of these structures . ^^^ The stimulated efflux was not significantly affected in astrocytes from GFAP / or vimentin / mice . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The S100B protein inhibits phosphorylation of GFAP and vimentin in a cytoskeletal fraction from immature rat hippocampus . ^^^ Analysis by two dimensional electrophoresis revealed a Ca2+ / calmodulin dependent and a Ca2+ / cAMP dependent inhibitory effect of S100B , ranging from 62 % to 67 % of control , on the phosphorylation of the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression levels of glial fibrillary acidic protein ( GFAP ) and vimentin and morphological features of astrocytes were used as indices of astroglial reactivity in adult transgenic versus wild type mice provided with ZnCl 2 in their water for 3 weeks . ^^^ In the striatum , the hippocampus , and the cervical spinal cord , the three CNS areas monitored , transgenic mice displayed enhanced GFAP mRNA and protein levels and elevated vimentin protein levels . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Alterations in temporal / spatial distribution of GFAP and vimentin positive astrocytes after spinal cord contusion with the New York University spinal cord injury device . ^^^ Astrocytes become reactive as a result of various types of lesions and upregulate 2 intermediate filaments , glial fibrillary acidic protein ( GFAP ) , and the developmentally regulated protein vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We suggest , on the basis of molecular mass , that the 54 , 51 and 43 . 5 kDa proteins could be the cytoskeletal proteins vimentin , GFAP and actin , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immature astroglia cells ( radial glia and astroblasts ) and astrocytes were immunostained using antibodies to the cytoskeletal proteins vimentin ( VIM ) and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP , S 100 and vimentin proteins in rat after cerebral post ischemic reperfusion . ^^^ In the present study astrocytes reactivity during cerebral post ischemic reperfusion was evaluated immunocytochemically by using antibodies to vimentin , glial fibrillary acidic protein ( GFAP ) and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Subsequent histological sections through these areas were stained with periodic acid Schiff , Sirius red , and Alcian blue ( basement membranes ) , and by immunohistochemistry to type 4 collagen ( basement membranes ) , von Willebrand factor ( endothelial cells ) , glial fibrillary acid protein ( GFAP ) and vimentin ( glial cells ) , S 100 protein ( perivascular glial cells ) , carbonic anhydrase isoenzyme 2 ( CAH 2 ) and CD 57 antigen ( Mller cells ) , and CD 68 antigen ( microglia ) . ^^^ The material which was accumulated to occlude the lumen of retinal capillaries displayed immunoreactivity to GFAP , vimentin , CD 57 antigen , and CAH 2 , but not to S 100 protein , suggesting that this material represents invaded Muller cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunoreactivity was strongly positive for vimentin and S 100 protein , less intense for the cytokeratins , and negative for smooth muscle actin , muscle specific actin , myoglobin , and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In adult rodents , Bergmann glia ( BG ) of the cerebellum continue to express vimentin together with glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression of CD 15 antigen ( also referred to as stage specific embryonic antigen , SSEA 1 , or Lewis ( 10 ) ) was analyzed in cerebellar cultures prepared from seven day old rats by double immunostaining with anti CD 15 mAb7A and cell specific antibodies to glial fibrillary acidic protein ( GFAP ) and Vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present report , by double immunofluorescence cytochemistry S 100B was localized to both GFAP / vimentin IFs and MTs as well as to centrosomes in U 251 glial cells . ^^^ In cells treated with the MT depolymerizing agent , colchicine , S100B remained associated with the rearranged GFAP IFs throughout the cell and , at the cell periphery , vimentin IFs . ^^^ Also , a preferential association of S100B with GFAP vs . vimentin IFs could be observed near the nucleus where colocalization of S100B with MTs was also maximal . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We demonstrate that reverse transcription PCR of RNA isolated from a single eye can detect the mRNAs for glial fibrillary acidic protein ( GFAP ) , vimentin , neurofilament light chain ( NF 68 ) and Thy 1 , all of whose promoters have been used to direct transgene expression in the CNS . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunostaining confirmed that these cells were positive for vimentin but negative for smooth muscle actin , GFAP , S 100 protein , and desmin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical analysis of sections revealed that the neoplastic cells were immunoreactive for glial fibrillary acidic protein ( GFAP ) , vimentin and S 100 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Histologically , the tumor was composed of poorly differentiated neuroectodermal cells , and these neoplastic cells showed a mild immunohistochemical reaction for GFAP and synaptophysin , and a moderate reactivity for neuron specific enolase and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Reactive astrocytes were induced by repeated seizures and their presence was confirmed by a qualitative increase in glial fibrillary acidic protein ( GFAP ) and vimentin immunoreactivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In response to injury of the central nervous system , astrocytes become reactive and express high levels of the intermediate filament ( IF ) proteins glial fibrillary acidic protein ( GFAP ) , vimentin , and nestin . ^^^ We have shown that astrocytes in mice deficient for both GFAP and vimentin ( GFAP / vim / ) can not form IFs even when nestin is expressed and are thus devoid of IFs in their reactive state . ^^^ Here , we have studied the reaction to injury in the central nervous system in GFAP / , vimentin / , or GFAP / vim / mice . ^^^ Glial scar formation appeared normal after spinal cord or brain lesions in GFAP / or vimentin / mice , but was impaired in GFAP / vim / mice that developed less dense scars frequently accompanied by bleeding . ^^^ These results show that GFAP and vimentin are required for proper glial scar formation in the injured central nervous system and that some degree of functional overlap exists between these IF proteins . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytic proteins GFAP , vimentin , S 100 , Glutathione S Transferase Yb ( GST Yb ) and neuronal protein TG 2 were detected by immunofluorescence . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Examination of various regions of the retina from the experimental PVR eye demonstrated that vimentin , glial fibrillary acidic protein ( GFAP ) , cellular retinaldehyde binding protein ( CRALBP ) , and beta amyloid precursor protein ( beta APP ) , which were present in the Mller cells of the retina from the control eye , increased their expression , while the antigenicity of glutamine synthetase ( GS ) , did not change ; these proteins were also present in the cells contained within the experimentally induced epiretinal membrane . ^^^ This suggests that a localized mechanism associated with epiretinal membrane formation induces the expression of alpha SMA in Mller cells while the increased expression of GFAP , beta APP , vimentin , and CRALBP are probably regulated via a more general mechanism . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These human GFAP inclusions did not contain the endogenous vimentin or beta tubulin , and the intermediate filament and microtubular networks of the transfected cells appeared normal . alphaB crystallin and hsp 25 were associated with the GFAP inclusions . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Subsequent immunocytochemical analysis showed that they stained positive for GFAP and vimentin , and negative for A2B5 , O 4 , GalC , and MAP 2 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Here , using a series of monoclonal antibodies that can specifically recognize distinct phosphorylated sites on glial fibrillary acidic protein ( GFAP ) and vimentin , phosphorylation sites by Rho kinase in vitro were revealed to be identical to in vivo phosphorylation sites on these intermediate filament ( IF ) proteins at the cleavage furrow in dividing cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : Pineal glands from 10 horses with uveitis and from 13 horses without uveitis were evaluated for histochemical ( H & E , collagen ) and immunohistochemical ( MHC class 2 antigen expression , infiltration of T and B lymphocytes , and glial fibrillary acidic protein ( GFAP ) and vimentin upregulation ) evidence of inflammation . ^^^ No differences in GFAP or vimentin immunoreactivity were noted in pineal glands from horses with or without uveitis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In order to determine the extent of microglial influx into the nervous system , we have examined their distribution within the human fetal spinal cord in relation to astrocytic and vascular development between 9 and 16 weeks of gestation , using conventional immunohistochemistry [ CD11b ; CD 45 ; CD 64 ; CD 68 ; ICAM 1 ; ICAM 2 ; VCAM 1 ; PECAM ; GFAP ; vimentin ] and lectin histochemistry [ RCA 1 ] . ^^^ This inward migration is associated with progressive vascularisation , ICAM 2 expression and co localises with GFAP and vimentin positive radial glia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NDGA could induce differentiation of these cells with the glial filaments increased , and the treated cells expressed more GFAP but less vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The majority of neoplastic cells were glial fibrillary acidic protein ( GFAP ) and vimentin positive , whereas S 100 protein labeled only individual cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Regulation of protein phosphorylation in astrocyte cultures by external calcium ions : specific effects on the phosphorylation of glial fibrillary acidic protein ( GFAP ) , vimentin and heat shock protein 27 ( HSP 27 ) . ^^^ Zero [ Ca2+ ] e increased total 32P incorporation into astrocyte protein and when this was normalized to 100 % , incorporation was significantly increased into glial fibrillary acidic protein ( GFAP ) , vimentin ( VIM ) and HSP 27 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GLT 1 protein expression within astrocytes is developmentally regulated , appearing first in vimentin positive radial glia at 60 and 71 days and then switching to GFAP positive parenchymal and perivascular astrocytes at 136 days . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
BMP 6 and 7 also promoted DNA synthesis and increased numbers of cells immunoreactive for vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunodetection of the cytoskeletal filament proteins , vimentin ( VIM ) , and glial fibrillary acidic protein ( GFAP ) , demonstrates that at 12 weeks the perivascular glial processes are mostly represented by VIM stained fibers of the radial glia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The astrocytic nature of the dye coupled cells was verified by double labeling of neurobiotin filled cells with antisera for the astrocytic filament proteins GFAP or vimentin . ^^^ While vimentin is the major astroglial filament in autumn , GFAP is strongly expressed in spring . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocyte maturation is followed by a switch between vimentin and glial fibrillary acidic protein ( GFAP ) expression , with the latter being recognized as an astrocyte maturation marker . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The proteins examined were alpha smooth muscle actin ( alphaSMA ) , h 1 calponin ( calponin ) , keratin 14 ( K 14 ) , beta subunit of S 100 protein ( S 100beta ) , vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ The MECs exhibited immunoreactivity for alphaSMA , calponin and K 14 , but not that for S 100beta , vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : Cells of ERM were studied by electron immunocytochemical techniques , using simple , double , and triple immunostaining for cytokeratins ( CK ) , vimentin ( Vim ) , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes may express three types of intermediate filament proteins : glial fibrillary acidic protein ( GFAP ) , vimentin , and nestin . ^^^ We used mice with targeted mutations in the GFAP or vimentin genes , or both , to study the impact of loss of either or both of these proteins on intermediate filament formation in cultured astrocytes and in normal or reactive astrocytes in vivo . ^^^ We report that nestin can not form intermediate filaments on its own , that vimentin may form intermediate filaments with either nestin or GFAP as obligatory partners , and that GFAP is the only intermediate filament protein of the three that may form filaments on its own . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial fibrillary acidic protein ( GFAP ) and vimentin staining , karyotypes , and growth curves were obtained for representative glioma cultures . ^^^ All cultures tested were positive for vimentin ( 29 / 29 ) while 62 % ( 18 / 29 ) were positive for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our results show that acidic calponin is particularly enriched in : ( 1 ) growth cones and submembranous fields of maturing cerebellar and cortical cells , where it codistributes with microfilaments and ( 2 ) glial cells in vivo , including radial glia , glia limitans , Bergmann glia and mature astrocytes , and ex vivo , where acidic calponin strongly colocalizes with intermediate glial fibrillary acidic protein ( GFAP ) and vimentin filaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used primary cultures of reactive astrocytes obtained from gelatin sponge implants in adult rat brains , a system that yielded highly purified , homogeneous cultures with > 95 % of cells positive for GFAP , vimentin , and S 100beta . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Paraffin , as well as semithin and ultrathin LR White sections were incubated with antibodies reacting to antigens located on the cell surface ( stage specific embryonic antigen 1 ; SSEA 1 ) , within the plasma membrane ( myelin basic protein ) , in the cytosol ( HNK 1 , S 100 protein ) , in the cytoskeleton ( GFAP , vimentin , neurofilament protein , INT FIL ) , and in the extracellular matrix ( laminin ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These studies revealed a strong expression of GFAP , vimentin , and CD 34 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The impact of genetic removal of GFAP and / or vimentin on glutamine levels and transport of glucose and ascorbate in astrocytes . ^^^ The importance of the intermediate filament ( IF ) proteins glial fibrillary acidic protein ( GFAP ) and vimentin for astrocyte function was studied by investigating astrocytes prepared from GFAP / and / or vimentin / mice . ^^^ The rate of glucose uptake through facilitative hexose transporters was not affected by depletion of GFAP or vimentin . ^^^ However , compared with wild type astrocytes , glutamine concentrations were increased up to 200 % in GFAP / astrocytes and up to 150 % in GFAP+ / astrocytes and this increase was not dependent on the presence of vimentin . ^^^ GFAP / astrocytes in culture still contain IFs ( made of vimentin and nestin ) , whereas GFAP / vim / cultured astrocytes lack IFs . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunofluorescence investigation identified specific labeling for vimentin and glutamine synthetase ( GS ) but not for glial fibrillary acidic protein ( GFAP ) in the mature pecteneal glia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Different pathomorphological methods , including especially immunohistochemistry with GFAP , vimentin , p 53 , Ki 67 , and topoisomerase 2 alpha if applied together , may at least partially help to overcome the problems of the differentiation of reactive and neoplastic gliosis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Alzheimer type 2 astrocytes were glial fibrillary acidic protein ( GFAP ) negative or only weakly positive , weakly S 100 positive , and vimentin negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical stains showed positive staining for S 100 protein , vimentin , GFAP , NSE , and CD 57 , and negative staining for Ki 67 , CD 34 , Neurofilament protein , cytokeratin , and EMA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
For example , supraoptic astrocytes display a radial glia like morphology and continue to express vimentin , together with GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
TGF alpha differentially regulates GFAP , vimentin , and nestin gene expression in U 373 MG glioblastoma cells : correlation with cell shape and motility . ^^^ To begin understanding the regulation and biological significance of changes in the expression of intermediate filament proteins in astrocytic tumors , we have recently shown that TGF alpha alters the protein level of glial fibrillary acidic protein ( GFAP ) , nestin , and vimentin in U 373 MG glioblastoma cells . ^^^ This differential regulation of GFAP , nestin , and vimentin gene expression indicates that TGF alpha induces further dedifferentiation of U 373 MG cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In addition , in cynomolgus monkey optic nerves immunohistochemical staining for alphaB crystallin , glial fibrillary acidic protein ( GFAP ) and vimentin was performed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In this study , the cytokinetics of glia in terms of the development of cerebellar dysplasia in the CVD rat was investigated using glial fibrillary acidic protein ( GFAP ) and vimentin immunohistochemistry . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Irradiated cells expressed the 2A progenitor cell specific markers : GFAP , vimentin and A2B5 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The morphological differentiation of cobblestone like podocytes into process bearing cells was followed by loss of the myofibroblastic marker , SMAA , de novo expression of desmin , and distribution of GFAP , vimentin and desmin into the processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunodetection of glial markers showed that transplanted astrocytes exhibited intense immunostaining for both glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) , whereas transplanted tanycytes were intensely immunostained for VIM , but GFAP negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
They expressed the glial fibrillary acidic protein ( GFAP ) , intermediate filament protein vimentin , and S 100 ( beta subunit ) protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Plectin is associated with intermediate filaments , such as glial fibrillary acidic protein ( GFAP ) and vimentin , which have an important role in the glial reactions after lesions . ^^^ GFAP and vimentin showed intense immunopositivity in both areas . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial and neuronal localization of ionotropic glutamate receptor subunit immunoreactivities in the median eminence of female rats : GluR2 / 3 and GluR6 / 7 colocalize with vimentin , not with glial fibrillary acidic protein ( GFAP ) . ^^^ To identify these cells as tanycytes , the median eminence was dual immunostained with glutamate receptors and glial cytoskeletal marker proteins , such as vimentin or glial fibrillary acidic protein ( GFAP ) . ^^^ Both GluR2 / 3 and GluR6 / 7 were shown to colocalize with vimentin , not with GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression of polymorphonuclear leukocytes ( PMNs ) and monocyte / macrophage ( Mo / Mo ) cells , and vimentin ( VIM ) positive astrocytes and glial fibrillary acidic protein ( GFAP ) positive astrocytes were compared between groups . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our results show that , during development of the rat cortex , synemin is expressed only in a subpopulation of astrocytic cells expressing GFAP as well as vimentin and nestin . ^^^ Unlike GFAP , however , synemin is not expressed in mature astrocytes and , unlike vimentin and nestin , synemin is not present in astrocytic precursors before GFAP expression . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
No alteration of the actin , tubulin , or vimentin components of the cytoskeleton was observed in either cell type , whereas a disruption of the glial fibrillary acidic protein filament ( GFAP ) network was noted in MVeGFP infected U 251 cells . ^^^ The relative amounts of GFAP present in infected and uninfected U 251 cells were quantified by image analysis of data sets obtained by confocal microscopy by using vimentin , another intermediate filament on which MVeGFP has no effect , as a control . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The results are discussed with respect to previous studies on glial fibrillary acidic protein ( GFAP ) and vimentin to show the heterogeneity of IFs expression in glioma cell lines . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Since , OPIDN is associated with alteration in the expression of several proteins ( e . g . , Ca2+ / calmodulin dependent protein kinase 2 ( CaM kinase 2 ) alpha subunit , tau , tubulin , neurofilament ( NF ) protein , vimentin , GFAP ) as well as their mRNAs ( e . g . , NF , CaM kinase 2 alpha subunit ) , we determined the effect of a single dose of DFP on the expression of one of the best known immediate early gene ( IEG ) , c fos . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
With the exception of some animals with end stage tumors , very little astroglia or microglia reactivity was detected in the wild type tumors as analyzed by immunocytochemistry using glial fibrillary acid protein ( GFAP ) , vimentin , human histocompatibility leukocyte antigen DR , OX 42 , and CD 68 specific monoclonal antibodies . ^^^ Astrogliosis , as indicated by a strong GFAP and vimentin immunoreactivity , was seen in the tumor scar area . ^^^ GFAP and vimentin reactivity was already present after the GCV treatment in tumors induced with 1 % BT4C tk cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The retinas were double labeled with either anti vimentin and anti long / medium wavelength sensitive ( anti L / M ) cone opsin or anti glial fibrillary acidic protein ( GFAP ) and biotinylated peanut agglutinin ( PNA ) . ^^^ RESULT : The hypertrophy of Muller cells was readily traced using antibodies to vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
For characterization of the ependymal cells we used antisera against S 100 protein , vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ The adult ependyma consisted of a ciliated , cuboid cell monolayer with short basal processes ; it displayed S 100 immunoreactivity but only scarce deposits of vimentin and no GFAP . ^^^ The fetal ependyma had the appearance of a pseudostratified epithelium with elongated nuclei and basal processes containing S 100 and vimentin from day 80 post coitum and GFAP from day 100 post coitum . ^^^ In explants , no differences were seen between the three culture media ; the ependyma became pseudostratified , developed basal processes and showed increasing amounts of S 100 and vimentin first , and subsequently also GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the cytoplasm of the plasmacytoid and spindle cells was moderately positive for vimentin and GFAP , whereas the buccal gland adjacent to the tumor was negative for these antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Comparative anatomy of the cerebellar cortex in mice lacking vimentin , GFAP , and both vimentin and GFAP . ^^^ In the cerebellum of adult mammals , glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) are coexpressed in Golgi epithelial cells ( GEC ) , also known as Bergmann glia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A reaction was present in the epithelial cytoplasm of the rete ovarii and epoophoron of mesonephric origin , for vimentin , GFAP , cytokeratin markers , ( AE1 / AE3 , MNF 116 ; Cam 5 . 2 , 34 beta E 12 and for the monospecific antibodies to cytokeratins 7 and 19 ) , heat shock protein 27 , in the cell membrane for HBME 1 , EMA and in the subepithelial collagen for collagen 4 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
These neuroblast like cells were negative for glial fibrillary acidic protein ( GFAP ) , 2 ; , 3 ; cyclic nucleotide 3 ; phosphohydrolase ( CNPase ) , and vimentin , suggesting that they were not differentiating as glia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tumor cells were strongly immunoreactive for S 100 protein , GFAP , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Nestin , vimentin , and glial fibrillary acidic protein ( GFAP ) are the three identified proteins constitutive of intermediate filaments present in astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Alterations in levels of mRNAs coding for glial fibrillary acidic protein ( GFAP ) and vimentin genes in the central nervous system of hens treated with diisopropyl phosphorofluoridate ( DFP ) . ^^^ We studied the effect of DFP admimistration ( 1 . 7 mg / kg / s . c . ) on the expression of Intermediate Filament ( IF ) proteins : Glial Fibrillary Acidic Protein ( GFAP ) and vimentin which are known indicators of neurotoxicity and astroglial pathology . ^^^ Northern blots prepared using standard protocols were hybridized with GFAP and vimentin as well as beta actin and 18S RNA cDNA ( controls ) probes . ^^^ The results indicate a differential / spatial / temporal regulation of GFAP and vimentin levels which may be due to the result of disruption of glial neuronal network . ^^^ On the other hand both GFAP and vimentin levels were upregulated from 2 days onwards in the non susceptible tissue cerebrum , implying protective mechanisms from the beginning . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Double immunolabelling of brain sections with vimentin confirmed co localization with glial fibrillary acidic protein ( GFAP ) , mGluR2 / 3 and mGluR 5 in reactive astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Upon completion of the cell cycle , analysis of glia fibrillary acidic protein ( GFAP ) and vimentin abundance , and glutamine synthetase ( GS ) activity showed that astrocytes had gained in expression of differentiation markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
By comparison with GFAP expression in gliomas and vimentin in meningiomas , the colocalisation of gangliosides and intermediary filament proteins is supposed . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical evaluation was performed in cases with rhabdoid foci using a panel of antibodies to pancytokeratin ( pan CK ) , CK 7 , CK 20 , epithelial membrane antigen ( EMA ) , S 100 protein , desmin , vimentin , neuron specific enolase ( NSE ) , muscle specific actin ( MSA ) , smooth muscle actin ( SMA ) , human melanoma , black 45 ( HMB 45 ) , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The development of astroglial cells and the effect of the retinohypothalamic tract on it were studied by vimentin and glial fibrillary acidic protein ( GFAP ) immunocytochemistry in the suprachiasmatic nucleus ( SCN ) of the rat . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Consistent desmin , alpha smooth muscle specific and sarcomeric actin , and vimentin immunoreactivity was demonstrated in the tumor cells , whereas cytokeratins , CD 30 , CD 31 , CD 34 , CD 45 , CD 68 , EMA , GFAP , HMB 45 and S 100 protein were negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The intermediate filaments expressed by cultured FS cells were GFAP and vimentin . ^^^ GFAP labeling was punctate and vimentin was organized as filaments . ^^^ All cultured S 100 cells were positive for vimentin , suggesting a mesenchymal origin for the cells , and all cultured S 100 positive cells were positive for GFAP , suggesting a neuroectodermal origin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytes associated with the SON , and identified with antibodies targeting glial fibrillary acidic protein ( GFAP ) or vimentin , were not colabeled with NR2B . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At 5 , 15 , and 28 days after cell injections , PVR was clinically quantified , and immunohistochemistry for OX 42 , ED 1 , vimentin ( VIM ) , glial fibrillary acidic protein ( GFAP ) , and cytokeratin was performed . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GAN corresponds to a generalized disorganization of the cytoskeletal intermediate filaments ( IFs ) , to which neurofilaments belong , as abnormal aggregation of multiple tissue specific IFs has been reported : vimentin in endothelial cells , Schwann cells and cultured skin fibroblasts , and glial fibrillary acidic protein ( GFAP ) in astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In addition , while Nm 23 R2 was colocalized and coimmunoprecipitated with vimentin in nondifferentiated cells , both isoforms were associated with GFAP in differentiated cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our approach uncovered four oligomeric proteins tubulin ( alpha and beta ) , glial fibrillary acidic protein ( GFAP ) , desmin , and vimentin that have conserved regions matching the consensus sequence . ^^^ In the type 3 intermediate filament proteins ( GFAP , vimentin , and desmin ) , this region corresponds to a portion of a coiled coil ( helix 2A ) , the structural element responsible for their assembly . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The proliferating cells were identified as astrocytes based on their expression of vimentin and GFAP . ^^^ The mitogenic effects of CRF seemed to be primarily on immature astrocytes determined by their differential expression of vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To address the requirements for the formation of desmin containing IFs both in quiescent and in transdifferentiated stellate cells , we used mice deficient for glial fibrillary acidic protein ( GFAP ) and / or vimentin , which are additional IF proteins present in stellate cells . ^^^ In this study , we show that desmin can not form full length bundles of IFs in the absence of both GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Unlike GFAP mRNA , vimentin , beta tubulin , and beta and gamma actin mRNAs were mainly confined to the cell bodies , with only occasional labeling seen in the processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In all opercula , GFAP , vimentin , and cellular retinaldehyde binding protein positive glia were present . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The retinal explants were then studied by histology and immunocytochemistry for glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Retinal glial ( Mller ) cells displayed gliosis as indicated by increased GFAP immunoreactivity and decreased immunoreactivity for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The present immunoperoxidase cytochemical study describes the distribution of glial intermediate filament molecular markers , glial fibrillary acidic protein ( GFAP ) and vimentin , in the brain and spinal cord of the adult lizard , Podarcis sicula . ^^^ While vimentin immunoreactive elements are almost completely absent in the brain except for a few diencephalic radial fibres , the spinal cord ependyma exhibits a clearly vimentin positivity and no GFAP staining . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A comparison of intermediate filament markers for presumptive astroglia in the developing rat neocortex : immunostaining against nestin reveals more detail , than GFAP or vimentin . ^^^ The present study compares the immunopositive elements in the developing rat cortex between the day of birth ( P 0 ) and the 18th postnatal day ( P 18 ) , after immunostaining against nestin , vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ Nestin immunostaining revealed more structural details than either vimentin or GFAP , or they together . ^^^ While vimentin immunostaining preferred radial glia and GFAP preferred astrocytes , nestin immunostaining detected both . ^^^ Nestin immunostaining revealed such structures which were not seen in GFAP or vimentin immunostained sections : cell body like structures ' hanging ' at the end the radial fibers , seeming to divide with their fibers , or having astrocyte like processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
De novo expression of vimentin , GFAP or peripherin leads to the assembly of an extended intermediate filament network in intermediate filament free SW13 / cl . 2 cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Co expression of GFAP , vimentin and cytokeratins in GL 15 glioblastoma cell line . ^^^ Glial fibrillary acidic protein ( GFAP ) , vimentin ( 6 ) and cytokeratin ( CK ) intermediate filament ( IF ) proteins were studied in glioblastoma cell line GL 15 . ^^^ The double immunofluoresce revealed that all CK positive cells express GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Brains were removed and processed to measure infarct volume , 70 kDa heat shock protein ( hsp 70 ) , glial fibrillary acidic protein ( GFAP ) and vimentin ( Vim ) immunoreactivity ( IR ) , and apoptosis using terminal deoxynucleotidyl transferase ( TdT ) mediated dUTP digoxigenin nick end labeling ( TUNEL ) assay . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry showed that both tumours were immunoreactive to vimentin and NSE , whereas GFAP , CAM5 . 2 , EMA , S 100 protein and synaptophysin were negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Within both germinative zones , BrdU labeled nuclei were associated with cell bodies immunostained for the neuronal marker polysialylated neural cell adhesion molecule , but not for the glial markers glial fibrillary acidic protein ( GFAP ) or vimentin ( VIM ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical results included : positive vimentin and collagen ( subtypes 1 , 2 , and 6 ) ; and negative NSE , S 100 , GFAP , amyloid A component , desmin , alpha sarcomeric actin , pancytokeratin , EMA , and von Willebrand factor . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemistry demonstrated that Ito cells expressed HHF 35 muscle actin , vimentin , desmin , glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) , chromogranin A and cytokeratins in normal livers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ca2+ / calmodulin dependent protein kinase 2 ( CaM kinase 2 ) alpha subunit , tau , tubulin , neurofilament protein ( NF ) , vimentin , GFAP ) and an immediate early gene ( e . g . c fos ) in DFP treated hens . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The panel of primary antibodies used included vimentin , MIC 2 , NSE , S 100 protein , leu 7 , neurofilaments , GFAP , and chromogranin A . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In mature differentiated astrocytes , vimentin is replaced by the IF protein glial fibrillary acidic protein ( GFAP ) . ^^^ In response to injury of the CNS in the adult , astrocytes become reactive , upregulate the expression of GFAP , and reexpress vimentin . ^^^ For this purpose , we cocultured wild type neurons on astrocytes from three types of knock out ( KO ) mice for GFAP or / and vimentin in a neuron astrocyte coculture model . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
They do not express vimentin , GFAP , or NG 2 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The IF proteins studied included the type 1 and type 2 cytokeratins K 8 , K 18 , and K 19 ; the type 3 proteins desmin , glial fibrillary acidic protein ( GFAP ) , peripherin , and vimentin ; and the type 4 neurofilament triplet protein L ( NF L ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor cells suggesting glial differentiation were positive for glial fibrillary acidic protein ( GFAP ) , S 100 , neuron specific enolase ( NSE ) , and vimentin . ^^^ PNET like components in the primary tumor were positive for NSE , GFAP , and S 100 , and weakly positive for vimentin and synaptophysin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GRX cells expressed vimentin , desmin , glial fibrillary acidic protein ( GFAP ) , and smooth muscle alpha actin ( SM alphaA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Spheroid cultures were established from four malignant glioma cell lines : U 87 , U 373 , MOG G CCM and A 172 ; harvested from culture at weekly intervals and stained with Haematoxylin and Eosin ( H & E ) , TdT mediated dUTP 10 nick end labelling ( TUNEL ) and by immunohistochemistry for vimentin , Glial Fibrillary Acidic Protein ( GFAP ) and Ki 67 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Astrocytic intermediate filaments : lessons from GFAP and vimentin knock out mice . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Radiolabeled aRNA from single neurons was used to probe cDNA arrays containing the low ( NFL ) , medium ( NFM ) and high ( NFH ) molecular weight neurofilament isoform , alpha internexin , desmin , vimentin , peripherin ( PRPH ) , nestin , and glial fibrillary acidic protein ( GFAP ) cDNAs . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the glial processes associated with the TZ , vimentin expression decreases , while that of GFAP increases . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor showed strong and diffuse positivity for vimentin , and EMA and GFAP were focally detectable . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cells were analyzed by immunocytochemistry , Western blot and immunoprecipitation using antibodies to Hsp 27 , alpha B crystallin , vimentin or GFAP . ^^^ GFAP and vimentin co localized with Hsp 27 in the cytoplasm . ^^^ GFAP and vimentin co localized with Hsp 27 and alpha B crystallin in the perinuclear area . ^^^ Western blot and metabolic labeling detected increased synthesis of Hsp 27 , GFAP and vimentin but no change in alpha B crystallin . ^^^ These results indicated that GFAP and vimentin associate with Hsp 27 and alpha B crystallin in ONH astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies of tumor cells showed positive immunoreactivity for vimentin and epithelial membrane antigen but were negative for GFAP , desmin , neurofilament , keratin , S 100 , CD 34 and CEA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The current histopathological and immunohistochemical study ( with a panel of antibodies for GFAP , vimentin , S 100 protein , MBP , NSE ) has been performed on biopsy specimens from 12 cases of GFAP immunopositive oligodendroglial tumours to evaluate their phenotypic characteristics . ^^^ The majority of cases exhibited negative staining for vimentin whereas there was no evident correlation between GFAP expression and other immunohistochemical markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Mononuclear and multinucleate tumour cells were both positive for vimentin and CD 34 ; one tumour exhibited focal S 100 protein and GFAP positivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used type 1 , 2 , 3 , 4 collagen and fibronectin to study extracellular components , and glial fibrillary acidic protein ( GFAP ) , S 100 protein , vimentin , and so forth to study cellular components . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In cultured glial cells , a 6 hr exposure to TMT ( 10 microM ) produced a morphological response of the cells and increased immunoreactivity for vimentin , GFAP , and AM . mRNA levels for TNFalpha , IL 1alpha , GFAP , vimentin , and AM were elevated within 3 6 hr of exposure . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Although a pulse like expression of Hsp 27 is observed in some microglial cells at day 1 PL , most Hsp 27 labeled cells are reactive astrocytes , which show GFAP overexpression and coexpress vimentin from day 3 PL . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
All the GFAP positive transplanted cells were negative for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used 10 human glioma cell lines , for which we characterized glial differentiation by means of quantitative RT PCR for nestin , vimentin , and GFAP mRNA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
After surviving 3 10 months , retinal specimens were examined with hematoxylin and eosin staining and immunohistochemical analysis of vimentin and glial fibrillary acidic protein ( GFAP ) expression . ^^^ In the host retina covering the graft , outer layers were degenerated , and vimentin labeled Mller cells in this area appeared short , disorganized , and displayed strong GFAP labeling . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The purely spindled tumor exhibited immunoreactivity for vimentin , actins , S 100 protein , EMA ( focally ) , and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In addition , the individual tumor cells showed strong cytoplasmic immunoreactivity with antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin , as well as nuclear and cytoplasmic staining with S 100 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The effects of meningeal fenestration on glial cells were examined by immunocytochemical procedures ( day 15 ) using the antibodies against two astrocyte markers : glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Vimentin , alphaSMA , and glial fibrillary acidic protein ( GFAP ) expression was detected by indirect immunofluorescence . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
OBJECTIVE : To observe immunohistochemical expression of S 100A1 , S 100A2 , S 100A4 , S 100A6 , S 100B , K8 . 12 , KL 1 , Vimentin , GFAP and NSE in pleomorphic adenoma of salivary gland , and to evaluate the differential localization of S 100 proteins and biological behaviour of neoplastic myoepithelial cells . ^^^ Nonluminal tumor cells had positive immunoreactivity for S 100B , as well as Vimentin , keratins detected by monoclonal K8 . 12 and KL 1 , GFAP and NSE . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Double labeling to glial fibrillary acidic protein ( GFAP ) and BrdU , or isolectin B ( 4 ) and BrdU identified astrocytes or microglia cells that proliferated ; immunoblotting and immunoreactivity to vimentin served for assess immaturity of astrocytic intermediate filaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The staining pattern was identical for the two nestin antisera and very similar to that of vimentin , while glial fibrillary acidic protein ( GFAP ) , immunoreactivity was absent from 9 . 5 week old forebrain . ^^^ In all tumors nestin immunoreactivity was confined to the cytoplasm of tumor cells and was co expressed with astrocyte markers vimentin , GFAP , and S 100 . ^^^ Vascular endothelial cells of all neoplasms also showed marked immunoreactivity for nestin and vimentin , whereas they were negative for GFAP and S 100 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Neuropathological examination , performed in one , included immunocytochemical studies of NeuN , synaptophysin , vimentin , and glial fibrillary acidic protein ( GFAP ) . ^^^ The architecture of the cerebellar cortex was imperfect but generally normal , and Bergmann glial cells had normal radial processes shown by vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
RESULTS : After 130 to 150 passages , SHG 44 cells were weakly positive for glial fibrillary acidic protein ( GFAP ) , but strongly positive for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Both cases on immunohistochemistry ( IHC ) showed reactivity for vimentin , S 100 protein and glial fibrillary acidic protein ( GFAP ) antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
However , in vivo hyperthermia does not appear to affect the morphology of the intermediate filament proteins GFAP , vimentin , or the beta tubulin component of microtubules in Bergmann glial cell processes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , tumour cells were positive for cytokeratin AE1 / AE3 , cytokeratin 14 , vimentin , calponin , S 100 protein and gliofibrillary acid protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Histopathologic study revealed a juxtapapillary choroidal melanocytoma The pigmented tumor cells stained positive for HMB 45 and vimentin but negative for S 100 , GFAP , NSE , and MNF 116 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
A similar motif is also present in other cytoplasmic intermediate filaments ( i . e . , vimentin , desmin , peripherin and GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We evaluated embryonal brain matter obtained from human 8 12 week old fetuses by means of flow cytometry on a panel including : nestin ; vimentin ; NeuN ; GFAP ; beta tubulin 3 ; CD 56 ; N Cad ; OB Cad ; HLA ABC ; HLA DR ; CD 34 , and annexin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
All tumor cells were strongly immunoreactive for vimentin and negative stained for glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , CD 117 and CD 34 showed diffuse strong expression , the positive rates were 98 . 7 % and 68 . 4 % respectively , alpha SMA , MSA , S 100 , PGP9 . 5 showed focal expression , the positive rates were 25 . 0 % , 19 . 7 % , 23 . 7 % and 17 . 1 % respectively , vimentin were all positive and desmin , GFAP , NF were all negative . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our data showed that after 30 min under simulated microgravity the cytoskeleton was damaged : microfilaments ( F actin ) and intermediate filaments ( Vimentin , Glial Fibrillary Acidic Proteins GFAP ) were highly disorganised , microtubules ( alpha tubulin ) lost their radial array , the overall cellular shape was deteriorated , and the nuclei showed altered chromatin condensations and DNA fragmentation . ^^^ The research was carried out by immunohistochemistry using antibodies to alpha tubulin , vimentin and GFAP , and cytochemical labelling of F actin ( Phalloidin TRIC ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tumors were evaluated for reactivity to the following six immunohistochemical markers : vimentin , pancytokeratin , glial fibrillary acidic protein ( GFAP ) , S 100 , neuron specific enolase ( NSE ) , and synaptophysin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
On immunohistochemistty the tumor showed strong Vimentin , S 100 and Glial Fibrillary Acidic Protein ( GFAP ) positivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Sarin causes early differential alteration and persistent overexpression in mRNAs coding for glial fibrillary acidic protein ( GFAP ) and vimentin genes in the central nervous system of rats . ^^^ We investigated the mRNA expression of the astroglial marker genes glial fibrillary acidic protein ( GFAP ) and vimentin to evaluate the fate of astroglial and neuronal cells , because reactive gliosis is very often used to assess the extent of CNS damage . ^^^ Total RNA was extracted and Northern blots were hybridized with cDNA probes for GFAP and vimentin , as well as 28S RNA ( control ) . ^^^ The data obtained indicate that a single dose of sarin ( 0 . 5 10 LD 50 ) showed induction in the transcript levels of GFAP and vimentin in the cortex , cerebellum , brainstem and midbrain , and spinal cord . ^^^ Both GFAP and vimentin were induced at 1 hour in all the tissues studied except brainstem , where moderate and high levels of GFAP induction were noted at 1 and 3 days . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies directed against alpha tubulin , actin , glial fibrillary acidic protein ( GFAP ) , vimentin , neurofilament ( 200 ) and beta catenin were used to investigate the cytoskeletal changes over a certain period of time . ^^^ After experimental detachment Mller cells quickly started to hypertrophy showing increased levels of intermediate filaments ( i . e . vimentin and GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Subsequently , immunohistochemical reactivities of the tumor cells for various cytokeratins , glial fibrillary acidic protein ( GFAP ) , S 100 protein , alpha smooth muscle actin ( SMA ) , and vimentin were similar to those of pleomorphic adenoma of the parotid gland . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Here we report that menin interacts with the type 3 intermediate filament ( IF ) proteins glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Deletion mutants of GFAP or vimentin involving the head domains of the molecules abolish the interaction with menin . ^^^ Endogenous menin is colocalized with GFAP and vimentin in glioma cells as determined by confocal microscopy . ^^^ Furthermore , a tailless GFAP deletion mutant , which disrupts the IF network , results in menin / GFAP / vimentin containing aggregates . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At the end of the experiment , the whole CNS of each animal was collected for histopathology and immunohistochemistry for apoptotic markers ( BAX , BCl 2 and CPP 32 ) and for glial fibrillary acid protein ( GFAP ; vimentin ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Pulse chase experiments of cultured astrocytes indicated that the Hm 3 GFAP encoded by Gfap ( hm 3 ) was unstable particularly in the absence of vimentin , another IF protein . ^^^ These results revealed the role of phosphorylation in turnover of GFAP and a synergistic role of GFAP and vimentin in the dynamics of glial filaments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In this study we immunohistochemically examined the expression of nestin and other intermediate filament proteins , including desmin , keratins ( Ks ) , glial fibrillary acidic protein ( GFAP ) , neurofilament , and vimentin in GISTs of different sites , esophageal leiomyomas and GI schwannomas . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunofluorescent analysis of two porcine epiblast derived cell lines demonstrated that the cells were positive for the expression of vimentin and the glial fibrillary acidic protein ( GFAP ) . ^^^ Similar analysis of a bovine epiblast derived cell line showed that the cells were positive for vimentin but that they did not express GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Processes of RG surrounding the SVZ / RMS could also be observed by immunostaining for vimentin , GFAP , and nestin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To elucidate the role of glia in this phenomenon , we investigated neuronal dispersion , astrocyte organization and expression of intermediate filaments of mature and immature astrocytes ( i . e . glial fibrillary acidic protein ( GFAP ) and vimentin , respectively ) in seven subjects with early febrile seizures ( F ( + ) ) and five subjects with other etiologies than febrile seizures ( F ( ) ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Incipient stages of histogenesis in the reaggregates and their interaction with host brain were investigated using light and electron microscopy , with antibodies against vimentin , glial fibrillary acidic protein ( GFAP ) , proliferating cell nuclear antigen ( PCNA ) , ferritin , as well as lectin ricinus communis agglutinin ( RCA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor cells were immunoreactive for GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
After surviving 6 174 days , eyes were examined in the light microscope , and grafts displaying the normal laminated morphology were labeled with antibodies against vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In this study , we investigated the effects of p 16 expression and RA treatment on the expression and distribution of actin , glial fibrillary acidic protein ( GFAP ) , and vimentin within the U 343 MG A astrocytoma cytoskeleton . ^^^ We showed that p 16 expression and RA treatment led to an increase in the expression of GFAP , as well as its reorganization but that it did not significantly affect actin or vimentin expression . p 16 induction in combination with RA treatment resulted in a decreased expression and activation of rhoA as determined by immunocytochemistry and Western blot analysis of soluble and insoluble fractions of cell lysates . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This study has investigated the interactions between growth factors and estrogens on DNA labeling and cytoskeletal protein [ glial fibrillary acidic protein ( GFAP ) and vimentin ] expression in 22 DIV astrocyte cultures treated for 24 or 36 h under different experimental conditions . ^^^ Western blot analysis for cytoskeletal proteins showed also changes of both GFAP and vimentin expression . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor is characterized immunophenotypically by a mesenchymal and neurogenic profile with positivity for vimentin , S 100 , and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The spinal cord tissues on postoperative days ( POD ) 5 , 7 , 10 , and 14 were processed to observe astrocytic , radial glial , and microglial differentiations by glial fibrillary acid protein ( GFAP ) , vimentin and ricinus communis agglutinin 1 ( RCA 1 ) stainings , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Id 1 expression defines a subset of vimentin / S 100beta positive , GFAP negative astrocytes in the adult rat pineal gland . ^^^ In the present study , double fluorescence immunocytochemical analysis was used to examine the co localization of Id 1 and Id 3 with both neuronal ( synaptophysin , betaIII tubulin ) and astrocytic markers ( GFAP , vimentin , S 100beta ) in the rat pineal . ^^^ In addition to localizing Id 1 and Id 3 protein to the melatonin producing pinealocytes , we have also made the novel observation that Id 1 , but not Id 3 , is highly expressed in a population of vimentin positive / S 100beta positive / GFAP negative astrocytes . ^^^ The pineal has been recognized previously as a unique region of the central nervous system in which a vimentin positive / GFAP negative glial phenotype is maintained in adult mammals . ^^^ The exclusion of Id 1 from GFAP positive cells , and expression in a population of vimentin positive pineal astrocytes is evidence of a role for Id 1 in the adult stabilization of one form of astrocyte . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tissue sections were labeled with antibodies to cone opsins , rod opsin , glial fibrillary acidic protein ( GFAP ) , vimentin , synaptophysin , cytochrome oxidase , and calbindin D 28K . ^^^ Mller cells remain remarkably unreactive ; they show essentially no proliferation , only negligible hypertrophy , and there is no increase in their expression of GFAP or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Mild upregulation of vimentin and glial fibrillary acid protein ( GFAP ) was seen in astrocytes adjacent to the fenestration , but no change in S 100 beta was evident . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
On immunohistochemical evaluation , hyaline cells were strongly and diffusely positive for S 100 protein , vimentin , pan ( CAM 5 . 2 ) and high molecular weight ( 34betaE12 ) cytokeratins ; these cells were focally positive for GFAP , maspin , neuron specific enolase , and cytokeratin 14 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ependymal cells in culture were labelled with glial markers ( S 100 , vimentin , GFAP , BLBP , 3A7 and 3CB2 ) and did not incorporate bromodeoxiuridine when this molecule was added to the culture media . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
IOP was recorded and eye tissues at 12 hours and 1 , 3 , 5 , 7 , or 14 days after laser were examined by immunohistochemistry with antibodies against glial fibrillary acidic protein ( GFAP ) , vimentin , S 100 , ED 1 , and OX 42 . ^^^ In the inner retina , there was a gradual and sustained increase in GFAP and S 100 immunoreactivity , but only a transient increase in vimentin immunoreactivity . ^^^ No remarkable changes in GFAP , vimentin , and S 100 immunoreactivity were noted at the optic nerve head ( ONH ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Histopathologic features were analyzed by routine HE stain and immunohistochemical stains for epithelial membrane antigen , cytokeratin , vimentin , smooth muscle actin , desmin , GFAP , S 100 protein , neurofilament protein , synaptophysin and alpha feto protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The second and fourth passage cells were stained with HE and anti GFAP , S 100 protein , Vimentin , and CD 34 antibodies . ^^^ These cells were positive in GFAP , S 100 protein and vimentin staining , and negative in CD 34 staining . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : With the use of three intermediate filament ( IF ) proteins , vimentin , glial fibrillary acidic protein ( GFAP ) , and cytokeratin ( CK ) , cytocentrifuge slides of 14 fresh vitreous aspirates were detected with immunohistochemical technique . ^^^ Immunocytochemical staining showed that 76 . 0 90 . 0 % cells stained for CK , 17 . 4 29 . 6 % cells expressed GFAP , and 80 . 1 91 . 0 % cells were positive for vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cell identity and changes in cell phenotype were confirmed by immunodetection of glial fibrillary acidic protein ( GFAP ) , cellular retinaldehyde binding protein ( CRALBP ) , vimentin , and alpha smooth muscle actin ( alpha SMA ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The canal of the uninjured cord is surrounded by four cell types : S 100 immunopositive ependymocytes , S 100 and glial fibrillary acidic protein ( GFAP ) immunopositive tanycytes , vimentin immunopositive dorsally located cells , and lateral and ventral liquor contacting neurons , which label for either gamma aminobutyric acid ( GABA ) or tyrosine hydroxylase ( TH ) . ^^^ The newly formed canal , which bridges the transection by day 10 but is not complete until about day 20 , is greatly enlarged ( < / =100 times ) and is dominated by ependymocytes that are vimentin immunopositive , but cells expressing GABA , TH , and GFAP do not appear until days 11 , 13 , and 16 , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Proliferating tumor cells were characterized immunohistochemically and found to express glial tumor markers including nestin , vimentin , glial fibrillary acidic protein ( GFAP ) , S 100 , and gap junction protein connexin 43 ( Cx 43 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Signet ring cells showed intense expression with GFAP , S 100 and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , some tumor cells were immunoreactive for synaptophysin , neurofilament , beta tubulin , chromogranin A , GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemical markers such as vimentin and glial fibrillary acidic protein ( GFAP ) were also employed to label the radial cells of glial lineage and to reveal the early pattern of astrocyte distribution . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The neoplastic cells showed diffuse cytoplasmic reactivity for EMA and vimentin , as well as positivity to GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Chlorotoxin tagged cells isolated by flow cytometry and cultured up to two passages exhibit positive labeling for GFAP and vimentin , but not for prolyl 4 hydroxylase ( fibroblast ) , A2B5 ( O2A progenitor ) , or OX 42 ( microglia ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The high salt Triton insoluble cytoskeletal fraction is enriched in neurofilament subunits ( NF H , NF M and NF L ) , vimentin and glial fibrillary acidic protein ( GFAP ) , while the low salt Triton insoluble cytoskeletal fraction contains detergent insoluble cytoskeletal elements such as intermediate filament subunits and tubulins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Using mice KO for GFAP and vimentin , we show that reinnervation occurs after an hemisection of the spinal cord , mainly through sprouting of controlateral intact serotoninergic and cortico spinal axons , thanks to the absence of glial reactivity . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , both oval and spindle cells were strongly positive for vimentin , smooth muscle actin , desmin and h caldesmon , negative for cytokeratin , EMA , CD 34 , CD 10 , CD 117 , HMB 45 , TTF 1 , BCL 2 , GFAP , calretinin , chromogranin , estrogen and progesterone receptors . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In all these assays , vimentin and glial fibrillary acidic protein ( GFAP ) showed comparable activities , versus desmin , which was almost inactive . ^^^ In addition , vimentin and GFAP exhibited much higher affinities for the Z DNA conformation of brominated , linear d ( CG ) ( 25 ) repeats than for the B DNA configuration of the unmodified oligonucleotides . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Moreover , vimentin , and to a lesser extent GFAP and desmin , interacted with the cruciform structures of inverted repeats inserted into a supercoiled vector plasmid , thereby changing their configuration via branch migration and sensibilizing them to processing by T 7 endonuclease 1 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Basal expression levels of GFAP and vimentin in unlesioned CNTF deficient mice were reduced by 66 and 37 % , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Robust neural integration from retinal transplants in mice deficient in GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Moreover , synemin was also found in astrocytes of optic nerves and non myelin forming Schwann cells , together with glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^ Blot overlays pointed to molecular interactions of synemin with desmin , vimentin , GFAP and keratin 5 and 6 , but not with keratin 14 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor cells were positive for glial fibrillary acidic protein ( GFAP ) , epithelial membrane antigen ( EMA ) , vimentin , and negative for cytokeratin , synaptophysin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
SK 3 immunoreactive structures were overlapped with glial fibrillary acidic protein ( GFAP ) , but not with vimentin , markers for glial cells and olfactory sensory axons , respectively . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor was positive for S 100 , vimentin and Schwann / 2E and was negative for GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP served as a marker for distal reactive gliosis and vimentin ( VIM ) for proximal gliosis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of antigens was examined with the use of single and double immunolabelling methods for GFAP , vimentin , LCA , CD 68 , HLA class 2 and MAC 387 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To identify cells and associated molecules that promote or impede regeneration , we compared expression of collagen type 4 , glial fibrillary acidic protein ( GFAP ) , and vimentin immunoreactivity ( IR ) at the lesion sites in combination with anterograde axonal tracing between animals with two types of transection . ^^^ Axonal regeneration occurred as early as 18 hours after transection ; regenerating axons penetrated vessel like structures with collagen type 4 IR at the lesion site , while reactive astrocytes coexpressing GFAP and vimentin IR appeared in the lesioned white matter . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tissues were immunochemically labeled for glial fibrillary acidic protein ( GFAP ) or vimentin to identify astrocytes , or for ED 1 to identify microglia . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP and vimentin increased with aging and in Alzheimer ' s disease ( AD ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
For the analysis of transplanted SPC , various histological and immunohistochemical staining methods were applied ( bisbenzidine , BrdU , antibodies against human nuclei , vimentin , beta tubulin , neurofilaments , GFAP ) , that allowed an independent evaluation of their state and differentiation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical analysis of the tumor revealed that most of the neoplastic cells were immunoreactive for S 100 protein , with less intensity for glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical staining for vimentin , pancytokeratin , CD 3 , CD79a , synaptophysin , S 100 , and neuron specific enolase was negative ; glial fibrillary acidic protein ( GFAP ) staining was equivocal . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Investigation into the astrocytic immunoreactivity to GFAP and vimentin in the brainstem of Wistar rats submitted to the ethidium bromide gliotoxic model ] . ^^^ OBJECTIVE : Astrocyte immunoreactivity to glial fibrillary acidic protein ( GFAP ) and vimentin ( VIM ) was investigated after EB injection . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Ependymal cells of type 2 and 3 walls were labelled with antibodies against S 100beta protein , vimentin , GFAP , BLBP and nestin . ^^^ Anti betaIII tubulin stained small cells in the subependyma and inside the GFAP and vimentin positive subjacent glial network . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The first population was composed of solid nests of polygonal eosinophilic or glycogen rich clear cells showing neoplastic myoepithelial immunocytological features , such as positivity for cytokeratins , vimentin , S 100 protein ( S 100 ) , alpha smooth muscle actin ( SMA ) and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of JUNV proteins was highly reduced in the presence of 2mM acrylamide while immunofluorescence staining of IF showed network disruption with the formation of cytoplasmic aggregates containing vimentin or glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical study of the excised periretinal membranes was performed with cytokeratins , GFAP , vimentin , CD 68 , CD 45 , and lysozyme antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical staining shows positive reaction for cytokeratin AE / 1 , CAM 5 . 2 , 34 beta E 12 , vimentin , smooth muscle actin , EMA , S 100 protein and oncogene cERB . b2 and negative for estrogen , progesterone , GFAP and chromogranin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Purified cells were immunostained with astrocyte markers : GFAP , vimentin , Pax 2 , A2B5 , nestin and NCAM . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : Idiopathic ERMs were excised at vitrectomy in 41 consecutive cases and stained for glial fibrillary acidic protein ( GFAP ) , vimentin , cytokeratin , desmin and actin . ^^^ RESULTS : Of the 41 ERMs , 31 ( 76 % ) were found to be fibroglial by light microscopy ; 40 ( 98 % ) stained for GFAP , 39 ( 95 % ) for vimentin , 10 ( 24 % ) for cytokeratin , 3 ( 7 % ) for desmin and 11 ( 27 % ) for actin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Prior to differentiation , most of the cells were nestin , Sox 2 , vimentin , and / or GFAP positive , and a subpopulation was doublecortin positive . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
As a consequence of retinal detachment , a `` mechanical ' ' injury to the retina , GFAP , and another intermediate filament protein , vimentin , dramatically increase in Mller cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Previous studies in teleosts have revealed the presence of the intermediate filaments vimentin ( Vim ) and glial fibrillary acidic protein ( GFAP ) in glial cells of the spinal cord and / or some brain regions , but there is no comprehensive study of their distribution and developmental changes in fishes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We found that the reaction surrounding transcranially implanted HFMs was significantly greater than that observed with intraparenchymally implanted materials including increases in immunoreactivity against GFAP , vimentin , ED 1 labeled macrophages and microglia , and several extracellular matrix proteins including collagen , fibronectin , and laminin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We used immunoperoxidase and immunofluorescence techniques to determine whether these transporters co localized with markers for blood vessels ( CD 34 ) , neurons ( microtubule associated protein 2 [ MAP 2 ] , SMI 311 ) , radial glia ( vimentin ) , or astrocytes ( glial fibrillary acidic protein [ GFAP ] , S100beta protein ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In this study interactions between growth factors and estradiol on DNA labeling and glial fibrillary acidic protein ( GFAP ) and vimentin expression in cultured astrocytes were investigated . ^^^ Western blotting showed also a modulation of GFAP and vimentin expression in treated astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The material was fixed in buffered formalin and embedded in paraffin and then stained with hematoxylin and immunostained for GFAP , cytokeratin and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Such distinctive nestin expressing cells were not seen in nestin stained control brains or ENU exposed brains stained for GFAP or vimentin , nor was such a cell seen in a stab wound model used to assess reactive astrocytosis . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies were positive for MIC 2 , NSE , C KIT , vimentin , Class 3 beta tublin and glycogen , but negative for NFP , synaptophysin , chromogranin A and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Upregulation of glial fibrillary acidic protein ( GFAP ) and vimentin immunoreactivity , a measure of reactive astrocytic response , were increased at 1 week after injury in the rats treated with electrical stimulation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The expression of the intermediate filaments GFAP , vimentin , and nestin was intensely upregulated immediately after transplantation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
It may partner with junD , NF kB , PEM , SMAD 3 , RPA 2 , FANCD 2 , NM23beta , nonmuscle myosin heavy chain 2 A , GFAP , and / or vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The cells expressed vimentin , glial fibrillary acidic protein ( GFAP ) , intermediate filament proteins expressed by neural progenitor cells , RG and astrocytes . ^^^ Lesioning the cerebral cortex did not change the expression of vimentin and GFAP in RG like cells , nor did it alter their morphology . ^^^ Our results show that cells expressing vimentin and GFAP ( in the radial glia astrocyte lineage ) are heterogeneous dorsoventrally in the walls of the LV . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
GFAP and vimentin expression was not effected by hyperoxia ; these intermediate filament proteins increased in Mller cells similar to that observed in control detachments . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
This study was designed to assess the interactions between growth factors and dexamethasone ( DEX ) on cytoskeletal proteins ( GFAP and vimentin ) expression in 25 days in vitro ( DIV ) astrocyte cultures . ^^^ An increase in GFAP and vimentin expression was observed after 12 h pretreatment with bFGF and subsequent treatment for 60 h with DEX . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Consistent with their osteoblastic lineage and malignant phenotype , all cell lines were immunoreactive to vimentin , osteopontin , PCNA , p 53 , MMP 2 and MMP 9 , while they were negative for cytokeratin , desmin , SMA , Factor 8 , NSE , GFAP , Rb and p 21 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Standard histology and immunohistochemical procedures for factor 8 related antigen , smooth muscle actin ( SMA ) , vimentin and glial fibrillary acidic protein ( GFAP ) were performed . ^^^ The extent of organization corresponded with increasing immunoreactivity for vimentin and GFAP , indicating their predominantly glial origin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To clarify the role of astrocytes in determining the success or failure of regeneration we compared expression of glial fibrillary acidic protein ( GFAP ) , vimentin and neurofilament ( NF ) immunoreactivity ( IR ) in the lesion between single and repeated transections . ^^^ However , by 24 h after a single transection , immature astrocytes coexpressing GFAP and vimentin IR appeared in the former astrocyte free area and NF positive axons crossed the lesion . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We found clear positivity to antibodies against mammalian tubulin , vimentin , GFAP , and actin , but not keratin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Most proliferating ventricular zone ( VZ ) cells are labeled with radial glial ( RG ) markers such as vimentin , glial fibrillary acidic protein ( GFAP ) , and glutamate astrocyte specific transporter ( GLAST ) antibodies . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : A series of eight cerebellar HBs were histochemically examined for the detection of mast cells and immunohistochemically for the expression of factor 8 related antigen ( FVIII RAg ) , CD 34 , vimentin , factor XIIIa ( FXIIIa ) , S 100 protein , glial fibrillary acidic protein ( GFAP ) , neuron specific enolase ( NSE ) , neurofilaments ( NF ) , synaptophysin , chromogranin , and somatostatin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Glial scar and axonal regeneration in the CNS : lessons from GFAP and vimentin transgenic mice . ^^^ Accordingly , knock out mice deficient in vimentin ( VIM ) and / or glial fibrillary acidic protein ( GFAP ) , the two major IF proteins of astrocytes , were investigated . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Rats were killed on day 7 after chemical denervation , and the reaction of glial cells was monitored within the olfactory bulb , using GFAP and vimentin immunohistochemistry . ^^^ Furthermore , GFAP and vimentin immunostaining in the glomerular layer of the olfactory bulb were diminished in the denervated and DHEA treated groups . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of glial intermediate filament molecular markers , glial fibrillary acidic protein ( GFAP ) , and vimentin , in the brain and spinal cord of the African lungfish , Protopterus annectens , was examined by light microscopy immunoperoxidase cytochemistry . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The combined detection of glial markers within cells present in the lesioned area indicated that , although they rarely express GFAP , the marker of mature astrocytes , NG 2 ( + ) cells located along the lesion borders frequently express nestin and vimentin , i . e . , two markers of immature astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Most of the tumor cells stained immunohistochemically for Cytokeratin , high molecular , CAM5 . 2 , and epithelial membrane antigen ( EMA ) , but were negative for alpha smooth muscle actin , vimentin , glial fibrillary acid protein ( GFAP ) , and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
However , two classes of abnormal giant cells were observed in brain sections from untreated aged Eker rats : ( a ) large dysmorphic pyramid like cells immunoreactive for NeuN , tuberin , and EAAC 1 in layers 4 6 ; and ( b ) abnormal cytomegalic cells immunoreactive for glial fibrillary acidic protein ( GFAP ) , vimentin , and nestin in deep cortical layers or along the white matter . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At 3 , 7 or 14 days post lesion , animals were sacrificed and their brains processed for immunohistochemistry to detect GFAP , vimentin , MAP 2 , S100B and nuclear Hoechst staining . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical studies demonstrated positive reactions for S 100 protein , cytokeratins , smooth muscle actin , vimentin , GFAP protein , p 53 protein and Ki 67 antigen , and allowed for establishing the final histopathological diagnosis of malignant myoepithelioma . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Agarose embedded sections ( 100 microm thick ) were double labeled for immunohistochemistry by confocal microscopy , with antibodies against rod opsin and GFAP ; vimentin and M / L cone opsin ; calbindin D and S cone opsin ; and cytochrome oxidase and synaptophysin . ^^^ All specimens demonstrated a marked upregulation of Muller cell and astrocyte expression of GFAP and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In undamaged retinas , we assayed whether intraocular injections of insulin , CNTF , or FGF 2 influenced glial expression of glial fibrillary acidic protein ( GFAP ) , neurofilament , RA 4 , vimentin and beta 3 tubulin by using immunocytochemistry on frozen sections . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Tooth cells that proliferate in vitro have polyclonal immunoreactivity ( IR ) for glial fibrillary acidic protein ( GFAP ) , growth associated protein ( GAP 43 ) , and vimentin , plus glial like ion channels . ^^^ Fibroblasts and undifferentiated mesenchymal cells in pulp had polyclonal GFAP IR and vimentin IR , while nerve fibers reacted only with polyclonal antibody . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In response to central nervous system ( CNS ) injury , and more discretely so also during aging , astrocytes become reactive and increase their expression of the intermediate filament proteins glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
At later time points , when astrogliosis is morphologically evident , nitrated hypertrophied reactive astrocytes are observed in the penumbra and the neurodegenerated area , displaying increased expression of GFAP and vimentin cytoskeletal proteins and of metallothionein 1 2 and Cu / Zn superoxide dismutase antioxidant proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present study , radial glia are investigated in vivo using the neuroepithelial markers nestin and vimentin and the gliogenic markers GLAST , BLBP , 3CB2 , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In vitro experiments showed that the intensity of immunofluorescent labeling for vimentin and glial fibrillary acidic protein ( GFAP ) was consistently decreased in astrocytes after transfection with a retrovirus carrying antisense complementary DNA ( cDNA ) for vimentin . ^^^ In vivo studies demonstrated that transfection with the retrovirus carrying the antisense cDNA vimentin inhibited the upregulation of vimentin and GFAP in stab wounds in rat cerebrum . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Differentiating cells expressed pancytokertin , vimentin , CD 31 , CD 56 , GFAP , nestin , and NeuN as well as CD 34 , and c Kit . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Thus , the conclusion must be drawn that the correlation of ganglioside patterns to the proliferation of astrocytic tumors is as poor as that of GFAP or vimentin expression , respectively . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Paraffin embedded brain sections were immunostained for glial fibrillary acidic protein ( GFAP ) and vimentin ( VN ) to identify glial cells ; for microtubule associated protein 2 ( MAP 2 ) and neuronal nuclear protein ( NeuN ) to document neurons ; for neurofilament ( NF ) to identify axons ; and for chondroitin sulfate ( CS ) , tenascin ( TN ) , and fibronectin ( FN ) to show the ECM . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Two of the citrullinated proteins were identified as vimentin and glial fibrillary acidic protein ( GFAP ) by using two dimensional gel electrophoresis and MALDI TOF mass spectrometry . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our immunohistochemical antibody panel included pan cytokeratin ( CK ) , low molecular weight CK ( CK8 / 18 ) , four basal cell type CKs ( 34betaE12 , CK5 / 6 , CK 14 , and CK 17 ) , vimentin antibodies , as well as antibodies to established ( SMA , CD 10 , p 63 , S 100 , maspin , calponin , GFAP , SM myosin ) , and novel ( CD 29 , 14 3 3sigma ) myoepithelial markers . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistological examination of the excised tissues was undertaken using confocal microscopy after labeling with antibodies to glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have also localized the expression of TNC mRNA , TNC , glial fibrillary acidic protein ( GFAP ) , vimentin and integrin beta 1 . ^^^ The proliferating border zone cells and pre migratory cells intensely expressed TNC mRNA , TNC , vimentin , GFAP and integrin beta 1 like immunoreactivity , while the migratory cells showed generally reduced expression except the front . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the perilesional rim , CTGF expression was confined to invading vimentin positive , glial fibrillary acidic protein ( GFAP ) negative fibroblastoid cells , endothelial and smooth muscle cells of laminin positive vessels , and GFAP positive reactive astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We observed that ditelluride in the concentrations of 1 , 15 and 50 microM induced hyperphosphorylation of the high salt Triton insoluble neurofilament subunits ( NF M and NF L ) , glial fibrillary acidic protein ( GFAP ) and vimentin , without altering the immunocontent of these proteins . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In primary rat astrocytes and Cos 7 cells , the mutant GFAP was incorporated into filament networks along with the endogenous GFAP and vimentin , respectively . ^^^ Although the presence of a suitable coassembly partner ( vimentin or GFAP ) reduced the potential negative effects of the R239C mutation on GFAP network formation , the mutation affected the stability of GFAP in cells in a dominant fashion . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We ascertain that luminal epithelial neoplastic cell were almost exclusively positive to antibody peculiar to epithelial differentiation ( AE1 / AE3 , MNF 116 , CK 8 , CK 19 , CK 20 , EMA ) and occasional to S 100 protein and negative to vimentin , alpha smooth muscle actin , calponin , Glial Fibrillary Acidic Protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Expression of nestin , vimentin , keratocan , alpha smooth muscle actin ( alphaSMA ) , beta 3 tubulin , neurofilament M ( NFM ) , and glial fibrillary acidic protein ( GFAP ) was examined in the sphere colonies and their progeny ( i . e . , cells migrated from sphere colonies ) , by immunocytochemistry and / or reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Most of the cells within these colonies expressed nestin and vimentin , whereas some expressed beta 3 tubulin , NFM , GFAP , and alphaSMA by immunocytochemistry . ^^^ Ninety one percent and 89 % of the progeny expressed vimentin and alphaSMA , respectively , whereas nestin was undetectable . beta 3 tubulin , NFM , and GFAP positive cells were detected in the progeny at the frequency of 7 . 2 % , 0 . 9 % , and 0 . 5 % , respectively . ^^^ Semiquantitative RT PCR showed that nestin , NFM , GFAP , and keratocan gene expression was higher in the sphere colonies , whereas vimentin and alphaSMA expression increased in the progeny . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor cells were immunoreactive to AE1 / AE3 , CK 19 and S 100 , were partially positive for CK 7 , CK 8 , GFAP and PCNA , but were negative for SMA , CK 13 , CK 14 and vimentin . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Besides the CD117 / c kit marker , t ICC expressed variously CD 34 , caveolins 1 & 2 , alpha SMA , S 100 , vimentin , nestin , desmin , NK 1 . t ICC were negative for : CD 68 , CD1a , CD62P , NSE , GFAP , chromogranin A , PGP9 . 5 , but IHC showed the possible existence of ( neuro ) endocrine cells in tubal interstitium . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the neoplasm showed a polyphenotype , including glial fibrillary acidic protein ( GFAP ) , S 100 , synaptophysin , chromogranin A , cytokeratin , vimentin , smooth muscle actin , and desmin . ^^^ The inclusion body was a densely packed , granulovesicular structure at the electron microscopic level , and was immunoreactive for vimentin , GFAP , desmin , and actin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Agarose embedded sections were immunostained with antibodies to nestin , vimentin , glial fibrillary acidic protein ( GFAP ) , glutamate aspartate transporter ( GLAST ) , rhodopsin , Ki 67 , and cyclin D 1 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We analyzed the distribution of intermediate filament molecular markers , glial fibrillary acidic protein ( GFAP ) , and vimentin in the brain and spinal cord of the adult brown anole lizard , Anolis sagrei . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immortal RLT PSC cells retained the phenotype of activated PSC proven by the expression of alpha smooth muscle actin ( alphaSMA ) , vimentin , desmin and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our results indicated that nearly 100 % of cells isolated expressed the astrocytic markers GFAP , NG 2 , Pax 2 , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In the present study the beginning of reactive astrogliosis in the adult , as shown immunocytochemically by upregulation of glial fibrillary acidic protein ( GFAP ) and vimentin , was seen two days after the left sensorimotor cortex lesion , being maximal during the first two weeks and declining by 30 days after the lesion . ^^^ Conversely , after the neonatal lesion a lack of gliotic scar was seen until 30 days postsurgery , although the pattern of GFAP and vimentin expression during recovery period was the same . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical markers such as S 100 , Leu 7 , HMB 45 , Melan A , CK , EMA , vimentin , GFAP , laminin , collagen 4 and MIB 1 were detected with the Envision immunohistochemical staining method . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Several subtypes of RG were co labeled with vimentin and other glial markers ( BLBP , GFAP , or GLAST ) and quantified in vitro . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Two of the citrullinated proteins were identified as a vimentin and GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Double labeling showed that most of these cells express vimentin , a marker for immature astrocytes and radial glia , but not brain lipid binding protein ( BLBP ) , a marker for radial glial cells , or glial fibrillary acidic protein ( GFAP ) , a marker for mature astrocytes . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The distribution of intermediate filament molecular markers , glial fibrillary acidic protein ( GFAP ) and vimentin , has been studied in the central nervous system ( CNS ) of the adult leopard gecko , Eublepharis macularius . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Sections were doubly labeled with antibodies directed against AR and one of the following : neurons ( immature , nestin ; mature , NeuN ) or astrocytes [ immature , vimentin ; mature , glial fibrillary acidic protein ( GFAP ) ] or mature oligodendrocytes ( mGalC ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Six weeks after grafting , the animals were sacrificed and transplants were retrieved either by Hoechst fluorescence or by immunohistochemistry for detection of glial fibrillary acidic protein ( GFAP ) and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Cell morphology and expression of alpha smooth muscle actin , choline acetyltransferase , CD 45 , glial fibrillary acidic protein ( GFAP ) , keratocan , nestin , neurofilaments , protein gene product 9 . 5 , tyrosine hydroxylase , and vimentin were examined . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The culture ( VC312R ) was characterised by immunocytochemistry and flow cytometry using antibodies against cluster of differentiation 44 ( CD 44 ) , glialfibrillary acidic protein ( GFAP ) , intermediate filament proteins ( Nestin and Vimentin ) , neural cell adhesion molecules ( NCAMs ) ( ERIC and UJ13A ) , ganglioside ( GD 3 ) and neuron glial 2 ( NG 2 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The tumor cells were strongly positive for GFAP , vimentin and S 100 protein , all of which stressed the foamy appearance of the giant cells . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
METHODS : ILM peeled from ten eyes during vitrectomy by infracyanine green ( ICG ) was studied immunohistochemically using the markers : GFAP , S 100 , and vimentin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , globules were negative for actin , keratin , vimentin , glially fibrillary acidic protein ( GFAP ) , and neurofilament . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
HISTOPATHOLOGY : Both tumors were composed of isomorphic rounded cells positive for synaptophysin , chromogranin and NSE , while some reacted for GFAP , vimentin and S 100 protein . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , they were investigated for AE 1 AE3 , MNF 116 , CK 8 , EMA , vimentin , alpha actin calponin , S 100 , GFAP , collagen 4 , and PCNA . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In 1 day cultured HSCs , synemin associated with other IF proteins ( GFAP , desmin , and vimentin ) , and with the focal adhesion proteins vinculin and talin , but not with alpha actinin or paxillin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In addition , Nordy treatment seemed to promote glioma cell differentiation , as evidenced by their reduced expression of vimentin and increased expression of GFAP . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The immunohistochemical study revealed intensive positivity of GFAP , vimentin , epithelial membrane antigen ( EMA ) , cytokeratin , connexin 26 and 32 , desmocollin 1 and neuronal cadherin . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
It has been studied in 50 neuroepithelial tumors , mostly gliomas of different malignancy grades , by immunohistochemistry , immunofluorescence , immunoblotting , and confocal microscopy and compared with GFAP and Vimentin . ^^^ In glioblastomas , Nestin distribution does not completely correspond to that of GFAP and Vimentin with which its expression varies in tumor cells in a complementary way , as confirmed by confocal microscopy . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical examination demonstrated labelling of luminal epithelial cells and myoepithelial cells , and mucinous , myxochondroid and cartilaginous tissues with antibodies to cytokeratin LU 5 , AE1 / AE3 , CK 14 , CALP , a SMA , vimentin , GFAP , and S 100 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Our immunohistochemical antibody panel included pan cytokeratin ( CK ) , basal cell type CKs ( 34betaE12 , CK5 / 6 , CK 14 , CK 17 ) and vimentin antibodies , antibodies to established ( SMA , CD 10 , p 63 , S 100 , maspin , calponin , GFAP , SM myosin ) , and novel ( CD 29 , 14 3 3sigma ) myoepithelial markers , as well as antibodies to CD 34 , desmin , h caldesmon , steroid receptors ( estrogen , progesterone , androgen ) , and EGFR ( Her 1 ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Antibodies included EMA , cytokeratins , smooth muscle actin ( SMA ) , desmin , vimentin , CD 34 , CD 10 , S 100 protein , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
We have studied the expression of alpha smooth muscle actin and intermediate filament proteins vimentin , desmin and glial fibrillary acidic protein ( GFAP ) by immunocytochemistry in HSC cultured for 2 or 7 days after isolation . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunocytochemistry used antibodies against markers of pluripotential stem cells ( CD 133 ) , multipotential progenitor cells ( nestin ) , antiapoptotic gene products ( Bcl 2 ) , immature neurons ( beta tubulin 3 , TUJ 1 ) , immature glia ( vimentin ) , mature neurons ( MAP 2 and NeuN ) , and astrocytes ( glial fibrillary acidic protein ; GFAP ) . ^^^ RESULTS : Balloon cells ( BCs ) were found to be immunoreactive to Bcl 2 ( 46 % ) , vimentin ( 41 % ) , Nestin ( 28 % ) , CD 133 ( 28 % ) , MAP 2 ( 27 % ) , GFAP ( 14 % ) , and TUJ 1 ( 10 % ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
To report the major intraretinal pathological changes in retinas with proliferative vitreoretinopathy ( PVR ) and retinal shortening , 13 human retinal samples from postoperative PVR after primary surgery for retinal detachment were immunostained for vimentin , glial fibrillary acidic protein ( GFAP ) , cytokeratins , and CD 68 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
The histologic diagnosis was made with immunohistochemical study in which the tumor showed positivity of vimentin , S 100 protein , neuron specific protein ( NSE ) , neurofilament protein ( NF ) and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
In this study , we investigate the effects of propionic acid ( PA ) , a metabolite accumulating in this disorder , on cytoskeletal reorganization , on cell viability , and on the in vitro phosphorylation of glial fibrillary acidic protein ( GFAP ) and vimentin in cultured astrocytes from cerebral cortex of neonatal rats . ^^^ In addition , the morphological alterations were accompanied by increased in vitro 32P incorporation into GFAP and vimentin recovered into the high salt Triton insoluble cytoskeletal fraction . ^^^ In conclusion , our results indicate that PA lead to cytoskeletal reorganization and to increased in vitro phosphorylation of Triton insoluble GFAP and vimentin . ^^^ On the basis of our results we could suppose that Triton insoluble GFAP and vimentin hyperphosphorylation could be implicated in the reorganization of cellular structure and these findings could be involved in the brain damage characteristic of propionic acidemia patients . . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemically , the tumor cells demonstrated reactivity for epithelial membrane antigen ( membranous ) and vimentin , with negative staining for S 100 protein , cytokeratin , CD 34 , glial fibrillary acidic protein ( GFAP ) , synaptophysin , and chromogranin A . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Neoplastic cells expressed vimentin and , multifocally , broad spectrum cytokeratins , but were negative for GFAP , NSE and Sl 00 antigen . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Immunohistochemical analysis revealed strong diffuse cytoplasmic immunoreactivity for vimentin and focal immunoreactivity for smooth muscle actin , whereas neoplastic cells did not stain for Factor 8 related antigen , Glial fibrillary acidic protein ( GFAP ) , or S 100 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
After incubation in neural differentiation medium , CB MSCs expressed the neural cell surface antigen A2B5 , the neurofilament polypeptide NF 200 , the oligodendrocyte precursor marker 04 , intermediate filament proteins characteristic of neural differentiation ( nestin and vimentin ) , as well as the astrocyte marker glial fibrillary acidic protein ( GFAP ) and the neural progenitor marker TUJ 1 . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
Neoplastic cells were positive for vimentin , nestin , microtubule associated protein 1B , S 100 protein , and glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
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Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA
Interacting proteins: P14136 and P08670	Pubmed	SVM Score :0.0
NA