| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation by Cdk 1 induces Plk 1 mediated vimentin phosphorylation during mitosis . ^^^ Cdk 1 also phosphorylates vimentin from prometaphase to metaphase , but its significance has remained unknown . ^^^ Here we demonstrated a direct interaction between Plk 1 and vimentin Ser 55 phosphorylated by Cdk 1 , an event that led to Plk 1 activation and further vimentin phosphorylation . ^^^ This elevation followed the Cdk 1 induced vimentin Ser 55 phosphorylation , and was impaired by Plk 1 depletion . ^^^ Taken together , these results indicated a novel mechanism that Cdk 1 regulated mitotic vimentin phosphorylation via not only a direct enzyme reaction but also Plk 1 recruitment to vimentin . . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| Cdk 1 phosphorylates vimentin Ser 55 from prometaphase to metaphase , leading to the recruitment of Polo like kinase 1 ( Plk 1 ) to vimentin . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| Visualization and function of vimentin phosphorylation by cdc 2 kinase during mitosis . ^^^ To investigate the role of intermediate filament ( IF ) protein phosphorylation by cdc 2 kinase during mitosis , we developed a monoclonal antibody 4A4 recognizing Ser 55 phosphorylated vimentin . ^^^ Western blotting indicated that this antibody reacted with vimentin phosphorylated by cdc 2 kinase but not with non phosphorylated vimentin or with vimentin phosphorylated by other kinases such as cAMP dependent protein kinase , protein kinase C , or Ca ( 2+ ) calmodulin dependent protein kinase 2 . ^^^ Immunofluorescent evidence using antibody 4A4 and biochemical analysis using vimentin Ser 55 peptide showed that the degree of disassembly of vimentin filament of various cell types at early mitotic phase correlated well with the amount of mitotically activated cdc 2 kinase . . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of synthetic vimentin peptides by cdc 2 kinase . ^^^ Synthetic peptide representing the site Ser 41 in vimentin , Leu Gly Ser 41 Ala42 Leu Arg 44 Arg Arg NH 2 , and its analogs in which Ala 42 was replaced by various amino acids were tested as substrates for cdc 2 kinase . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| Temporal change of vimentin phosphorylation by PKC differed form changes by cdc 2 kinase . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| To examine structural features of proline which are essential for the proline directed phosphorylation by cdc 2 kinase or cdk 5 , we prepared the peptide representing the cdc 2 kinase phosphorylation site at Ser 55 in vimentin [ Ser Leu Tyr Ser Ser Ser 55 Pro56 Gly Gly 58 Ala Tyr NH 2 ] , the peptide containing arginine in place of Gly 58 , and their derivatives containing various N methylamino acids or proline homologs in place of Pro 56 , and tested them as substrates for the kinases . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| Visualization of mitotic radial glial lineage cells in the developing rat brain by Cdc 2 kinase phosphorylated vimentin . ^^^ Here we examined mitotic radial glial lineage cells in the rat brain E 17 P7 , using the monoclonal antibody 4A4 , which recognizes vimentin phosphorylated by a mitosis specific kinase , cdc 2 kinase . ^^^ |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06493 and P08670 |
Pubmed |
SVM Score :0.0 |
| NA |
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