| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| Pathways downstream of Shc and Grb 2 are required for cell transformation by the tpr Met oncoprotein . ^^^ Utilizing this mutant , together with additional Tpr Met mutants containing Tyr to Phe substitutions , we have demonstrated that transformation of Fr3T3 fibroblasts by the Tpr Met oncoprotein is dependent upon pathways downstream of Shc and Grb 2 and that pathways downstream of phosphatidylinositol 3 ' kinase , phospholipase Cgamma , and SHPTP2 / Syp are insufficient for transformation . . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| Following Met activation , alpha6beta4 is tyrosine phosphorylated and combines with Shc and PI3K , generating an additional signaling platform that potentiates HGF triggered activation of Ras and PI3K dependent pathways . ^^^ In the presence of an alpha6beta4 mutant defective for Shc recruitment , Met can not sustain HGF mediated responses . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| IL 4 did not enhance HGF dependent tyrosine phosphorylation of c Met or Shc . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| HGF stimulated Gab 1 association with c Met , Grb 2 , SHP 2 , PI3K , Shc , Crk isoforms and CrkL , but not with PLCgamma 1 . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| After ligand induced tyrosine phosphorylation , the HGF receptor associates with the Shc adaptor , via the SH 2 domain . ^^^ Site directed mutagenesis of the HGF receptor indicates that phosphotyrosines Y1349VHV and Y1356VNV can work as docking sites for Shc . ^^^ After stimulation of the HGF receptor , Shc is phosphorylated on Y317VNV , generating an high affinity binding site for Grb 2 ( Kd = 15 nM ) . ^^^ These data show that Shc is a relevant substrate of the HGF receptor , and works as an ' amplifier ' of the motogenic as well as of the mitogenic response . . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation was performed with antisera to signal transducers and activators of transcription 1 ( STAT 1 ) , STAT 3 , Janus kinase 1 ( Jak 1 ) , Shc , Grb 2 , Sos 1 , and HGF receptor ( met ) . ^^^ Phosphorylated HGF receptor coimmunoprecipitated with Shc , Grb 2 , and Sos 1 . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| Some of the Shc associated phosphoproteins ( EGFR , PDGFR , erbB 2 , Met , bcr abl , H 4 ret ) bound both the Shc and Grb 2 SH2 domains in vitro ; others ( p 175 ; p 70 p80 ) only the Shc SH 2 domain and yet others ( p 140 ) only the Grb 2 SH3 domains . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| We have generated variant forms of the Tpr Met oncoprotein with the ability to bind individually to the p 85 subunit of PI3 ' K , PLCgamma , or to the Grb 2 or Shc adaptor proteins . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| The Shc adaptor protein is critical for VEGF induction by Met / HGF and ErbB 2 receptors and for early onset of tumor angiogenesis . ^^^ Moreover , the use of fibroblasts derived from ShcA deficient mouse embryos , demonstrated that Shc was essential for the induction of VEGF by the Met / hepatocyte growth factor RTK oncoprotein and by serum derived growth factors . ^^^ Together , our findings identify Shc as a critical angiogenic switch for VEGF production downstream from the Met and ErbB 2 RTKs . . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| Oncogenic Met receptor induces cell cycle progression in Xenopus oocytes independent of direct Grb 2 and Shc binding or Mos synthesis , but requires phosphatidylinositol 3 kinase and Raf signaling . ^^^ We show that cell cycle progression and activation of MAPK and JNK mediated by the oncogenic Met receptor , Tpr Met , are dependent on its kinase activity and the presence of the twin phosphotyrosine ( Y 482 & Y 489 ) residues in its C terminus , but that the recruitment of Grb 2 and Shc adaptor proteins is dispensable , implicating other signaling molecules . ^^^ However , using Met receptor oncoproteins engineered to recruit specific signaling proteins , we demonstrate that recruitment of Grb 2 or Shc adaptor proteins is sufficient to induce cell cycle progression and activation of MAPK and JNK , while the binding of phospholipase Cgamma or phosphatidylinositol 3 kinase alone fails to elicit these responses . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| Using variant forms of Tpr Met that are engineered to recruit a specific signaling molecule of choice , we demonstrate that the sole recruitment of either the Grb 2 or the Shc adaptor protein is sufficient to induce ectopic structures and anterior reduction , while the recruitment of PI 3Kinase ( PI 3K ) is necessary but not sufficient for this effect . ^^^ |
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| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08581 and P29353 |
Pubmed |
SVM Score :0.0 |
| NA |
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