Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.66445036
Furthermore , in CD 8 ( + ) spleen cells of E 14 group of 3 week old chickens , the expression of beta AR mRNA and the relative increase of intracellular cAMP stimulated with NE were significantly decreased . 0.66445036^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The genes encoding lysozyme , lactoferrin , cathelicidin antimicrobial peptide ( hCAP18 / LL 37 ) , cathepsin G , bactericidal / permeability increasing protein , azurocidin ( CAP37 / heparin binding protein ) , and neutrophil elastase were each found to be single copy per haploid genome . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Sensitivity was measured using the cAMP response of brain slices to norepinephrine ( NE ) . ^^^ The cAMP response of liver homogenates to NE was measured . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
No responses were detected when norepinephrine ( NE ) , glutamate , cAMP , substance P , or dopamine were applied to the SCGN of either age group . 6 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The cAMP response was evoked in vitro by various neurotransmitters including norepinephrine ( NE ) , histamine , serotonin , dopamine , acetylcholine , and gamma aminobutyric acid ( GABA ) . ^^^ The cAMP response to NE and histamine was enhanced by ethanol withdrawal . ^^^ GABA inhibited the NE stimulated cAMP response possibly through the hyperpolarizing action of GABA . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Adenosine 5 ' monophosphate produced a very slight increase in nerve stimulated release of NE and DBH activity in concentrations which inhibited pressor responses considerably . cAMP produced slight inhibition of pressure responses but failed to influence the release of either NE or DBH activity during nerve stimulation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effects of both chronic ethanol treatment and 3 days of ethanol withdrawal on the cyclic adenosine 3 ' : 5 ' monophosphate ( cAMP ) response of cerebral cortical slices to norepinephrine ( NE ) were studied . ^^^ ED 50 cAMP responses for each group were determined using graded doses of NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In the isolated perfused rat heart , the dose related cardiostimulation produced by norepinephrine ( NE ) or calcium chloride ( Ca2+ ) was followed by a corresponding increase in coronary flow ( CF ) and in the cardiac level of adenosine 3 ' , 5 ' cyclic phosphate ( cAMP ) . ^^^ Prolonged prostaglandin E 2 ( pge 2 ) infusion did not change the basic force of contraction , CF , or cAMP level but when NE or Ca2+ were administered , only the responses of the CF and the cAMP were diminished . ^^^ With NE or Ca2+ , during Dx both the changes in CF and cAMP were augmented compared to the nontreated hearts . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Cyclic adenosine 3 ' , 5 ' monophosphate ( cAMP ) accumulation and cation transport were measured in duck erythrocytes after stimulation by norepinephrine ( NE ) or shrinkage induced by exposure to hypertonic media ( S ) . ^^^ NE elicited a rapid rise in cellular cAMP and 42K influx . ^^^ At concentrations of NE below 3 10 10 ( 8 ) M ( the concentration at which 42K influx saturates ) , there was good correlation between the magnitude of the permeability change and the increment in cAMP . ^^^ The data are discussed in terms of a model in which S and NE activate a final common transport pathway by different mechanisms , which in the case of S does not involve cAMP . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Pimozide was found to be more specific than haloperidol as it did not effect norepinephrine ( NE ) elicited formation of [ 3H ] cAMP , while the latter blocker caused a 48 % reduction of the stimulation by NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Experiments were conducted to determine if stressful procedures , which increase brain norepinephrine ( NE ) release in rats , lower the responsiveness of central noradrenergic receptors as measured by the catecholamine ( CA ) induced cAMP accumulation in hypothalamic and cerebral cortical slices . ^^^ These include ( a ) the inhibition of phosphodiesterase activity with isobutylmethylxanthine , which may have obscured possible adaptive changes in cAMP degradation and / or adenosine dependent adrenergic receptors ; ( b ) a low initial responsiveness to NE in these animals as suggested by the greater case in inducing supersensitivity with reserpine than subsensitivity with amphetamine ; and ( c ) the use as a test agent of exogenous NE which may stimulate a far broader population of receptive sites in brain slices than are activated during stress by the local release of endogenous NE . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Prior studies indicate ethanol feeding induces adrenergic subsensitivity in vitro , as measured by cyclic adenosine monophosphate ( cAMP ) response of tissues to norepinephrine ( NE ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Effects of exogenous PG E 1 on the level of human plasma norepinephrine ( NE ) , dopamine beta hydroxylase ( DBH ) activity , cAMP , cGMP , free fatty acids ( FFA ) as well as pulse rate and blood pressure were studied . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The response of cAMP to norepinephrine ( NE ) was measured in hypothalamic tissue and pineal glands of cycling female rats killed on the mornings of proestrus and diestrus ( in 4 day cycles ) or diestrus 2 ( in 5 day cycles ) . ^^^ Chopped hypothalamic tissue incubated in vitro revealed a greater response of cAMP levels to NE on proestrus compared to diestrus in the anterior hypothalamic preoptic area , but no significant changes in the middle or posterior hypothalamus . ^^^ In an effort to reproduce the changes noted on proestrus , ovariectomized animals were injected with estradiol benzoate for 2 days , but no effects of the treatment on the cAMP response to NE were found . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Local cerebral ischemia was produced in rats by internal carotid artery injection of 35 mu carbon microspheres , and brain norepinephrine ( NE ) , dopamine , and cyclic adenosine 3 , 5 monophosphate ( cAMP ) were measured in embolized and intact hemispheres at intervals up to four hours . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
With incubated tissue slices ( rat and rabbit cortices ) CPZ CH 3 , 7 OH CPZ CH 3 , beta OH CPZ and butaclamol displayed a capacity to inhibit either NE or H induced accumulation of adenosine cyclic 3 ' , 5 ' monophosphate ( cAMP ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE , forskolin , and 8 ( 4 chlorophenylthio ) cAMP all increase Vmax of the Ca2+ response curve of ATP . ^^^ We conclude that NE potentiates the ATP induced Ca2+ transient by increasing cAMP levels and that one of the early events is the increase of the inward Ca2+ current during the action potential . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We previously demonstrated that norepinephrine ( NE ) induction of cAMP accumulation in slices of the preoptic area ( POA ) and middle hypothalamus ( MH ) is reduced by in vivo administration of progesterone to estradiol primed rats , apparently by eliminating alpha 1 receptor augmentation of beta receptor stimulated cAMP formation . ^^^ The present studies examined whether in vitro exposure to progesterone would also depress NE stimulated cAMP synthesis . ^^^ Pre incubation of slices with progesterone for as little as 5 min significantly suppressed NE stimulated cAMP formation by greater than 60 % . ^^^ This effect was estrogen dependent in that progesterone in vitro did not inhibit NE stimulated cAMP accumulation in slices from ovariectomized rats not pretreated with estradiol . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These data indicate that aqueous humor NE per se does not cause the transient IOP decrease after superior cervical ganglionectomy and cAMP mediated ocular activities may be involved in this change in IOP . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These results suggest that cAMP inhibits NE induced contraction , at least partly , by inhibiting the alpha adrenoceptor mediated signal transduction and high K ( + ) induced contraction by decreasing Ca2+ sensitivity but not Ca2+ influx . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Alpha adrenergic receptor sensitivity was measured in platelets by the specific binding of [ 3H ] dihydroergocryptine and the NE inhibition of prostaglandin E 1 ( PGE 1 ) stimulated cyclic AMP ( cAMP ) production . ^^^ PGE 1 stimulated cAMP production was half as great in hypertensive as in normotensive men , while NE inhibition of PGE 1 stimulated cAMP production was similar in hypertensive and normotensive men and women . [ 3H ] Dihydroergoeryptine binding in female hypertensives , and PGE 1 stimulated cAMP in male hypertensives did not differ from that in sex matched controls . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effect of norepinephrine ( NE ) on cAMP accumulation in primary glial cultures is mediated by two functionally opposing receptor subtypes . beta Adrenergic receptor activation increases cAMP formation , whereas simultaneous alpha 2 adrenergic receptor activation partially inhibits this effect . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In parallel to the stimulation of GnRH release , NE also rapidly increased ( first observed at 60 sec ) the intracellular concentration of cAMP in isobutylmethylxanthine pretreated GT 1 1 and GT 1 7 cells in a dose dependent fashion . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Relaxing effect of FK 336 on NE response was inhibited by methylene blue , NG , K ( + ) channel inhibitors and acetylcholine ( ACh ) , and potentiated by M & B 22 , 948 and theophylline . 8 Br cGMP and dibutyl cAMP had no effect . 5 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
It is unlikely that the inhibitory effect of alpha 1 adrenergic stimulation on NE or forskolin elevated ICa is mediated through activation of Ca dependent protein kinase C or changes in intracellular free Ca ( pCa = 8 . 5 , EGTA 5 mM ) or cAMP dependent phosphodiesterase . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In primary glial cultures , norepinephrine ( NE ) activates both beta adrenergic receptors to increase cAMP formation and alpha 2 adrenergic receptors to partially inhibit this response . ^^^ NE inhibited the cAMP response to ISO with an apparent EC 50 of 38 + / 1 . 2 nM . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Although the cAMP level was not changed by norathyriol , the cGMP level was increased by a high concentration of norathyriol ( 400 microM ) . [ 3H ] Inositol monophosphate formation caused by NE was not affected by norathyriol at concentration of either 100 or 400 microM . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These results suggest that the ability of NPY to potentiate alpha 2 adrenoceptor induced inhibition of [ 3H ] NE release in discrete brain regions does not depend on the reductions in cAMP . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In hypoxic cells incubated with 5 mM glucose , the addition of 1 microM ( ) norepinephrine ( NE ) increased cAMP generation from 29 . 3 + / 10 . 6 to 54 . 2 + / 16 . 1 pmol / 35 mm plate ( n = 5 , P less than 0 . 025 ) ; upon reoxygenation cAMP levels remained elevated above control ( n = 5 , P less than 0 . 05 ) . ^^^ In contrast , NE stimulated cAMP content in glucose deprived hypoxic myocytes fell by 31 % ( n = 5 , P less than 0 . 05 ) and did not return to control levels with reoxygenation . beta AR agonist affinity assessed by ( ) isoproterenol displacement curves was unaltered after 2 h of hypoxia irrespective of glucose content . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Additional studies revealed that indomethacin ( 10 ( 6 ) M ) , isoproterenol ( 10 ( 5 ) M ) , forskolin ( 10 ( 5 ) M ) , and dibutyryl cAMP ( 1 mM ) had no effect on [ Ca2+ ] 1 in ciliated HNE cells . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Preincubation with Con A , 0 . 5 micrograms / ml , attenuated 1 microM ( ) norepinephrine ( NE ) induced downregulation of beta adrenergic receptors and resulted in a 50 % augmentation of cAMP accumulation stimulated by 1 microM NE . ^^^ The muscarinic cholinergic agonist carbachol ( 1 100 microM ) decreased 1 microM NE stimulated cAMP generation by an average of 32 % ( n = 7 , P less than 0 . 05 ) ; preincubation with Con A further enhanced the inhibitory effect of carbachol by 18 % ( n = 7 , P less than 0 . 05 ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
It was found that selective blockade of the metabolism of glial cells by fluorocitrate infusion produced a virtually complete ( 90 % ) inhibition of the cortical cAMP response to norepinephrine ( NE ) . ^^^ Pharmacological experiments showed that the response was selectively antagonized by a beta 1 receptor blocker which also selectively antagonized the cAMP response to NE in brain slices known to be localized in glial cells . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We measured the release of [ 3H ] norepinephrine ( NE ) induced by field electrical stimulation , NE stimulation of inositol phosphates [ ( 3H ] IP ) accumulation in the presence of LiCl and isoproterenol induced accumulation of cAMP in hippocampal slices taken from rats electrically kindled at stages 2 and 5 in the dorsal hippocampus . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In addition to target effect , we also tested the effect of elevated intracellular cAMP level on NE uptake versus GABA uptake . ^^^ Increasing the intracellular cAMP level , by application of the membrane permeable analog dibutyryl cyclic AMP ( DbcAMP ) , resulted in a selective stimulation of NE uptake , due to enhanced survival of noradrenergic neurons . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In addition to the effect of target , we also tested the effect of elevated intracellular cAMP levels on NE uptake versus GABA uptake . ^^^ Increasing intracellular cAMP resulted in selective stimulation of NE uptake . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Each agonist increased cellular cAMP , with relative potencies ( IC50S ) of ISO ( 0 . 5 microM ) greater than EPI ( 6 . 3 microM ) greater than NE ( 56 microM ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effects of norepinephrine ( NE ) , carbachol ( CCh ) , NaF , 3 isobutyl 1 methylxanthine ( IBMX ) , and high K+ concentration ( 80 mM ) depolarization on inositol trisphosphate ( IP 3 ) accumulation , cyclic AMP ( cAMP ) formation , and contraction were investigated in the dilator and sphincter smooth muscles of the sympathetically denervated as well as the normal rabbit eye . ( a ) In the denervated dilator muscle , NE stimulated IP 3 production and contraction are enhanced . ( b ) In the sphincter muscle of rabbits that have undergone sympathetic denervation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
To determine if Li has similar effects on Gi in the central nervous system , we measured extracellular ( EC ) cyclic adenosine monophosphate ( cAMP ) in rat brain by in vivo microdialysis , revealing a dose dependent increase in cAMP by norepinephrine ( NE ) antagonized by propranolol . ^^^ Chronic ( 4 week ) Li doubled basal EC cAMP , while decreasing the fractional response to 100 microM NE . ^^^ Thus , using in vivo microdialysis , we observed the reported reduction in NE stimulated AC activity , but only as a function of elevated basal cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These investigations suggest that NE elevates K+ flux , probably through a large conductance K ( Ca ) channel , that NE acts through alpha 1 and beta adrenergic receptors , and that Ca2+ and cAMP act together through a biochemical `` AND ' ' gate to mediate the effects of receptor activation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The role of cAMP in mediating the stimulatory effects of norepinephrine ( NE ) on testosterone ( T ) production by hamster testes in vitro was examined using tissue from both gonadally active and gonadally regressed hamsters . ^^^ In incubations of regressed testes from short photoperiod exposed hamsters , NE stimulated accumulation of cAMP in media and tissue . ^^^ In incubations of active testes from long photoperiod exposed animals , NE stimulated cAMP in the media but not in the tissue , and potentiated the effect of hCG on the accumulation of cAMP only in tissue . ^^^ When added to incubations with NE and hCG , propranolol , but not prazosin , reduced cAMP levels in media and tissue . ^^^ NE stimulates cAMP production via its action at the beta receptors and T production via its action at the alpha receptors . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In rat pinealocytes , norepinephrine ( NE ) increases cAMP and cGMP accumulation through a synergistic dual receptor mechanism involving alpha 1 and beta adrenergic receptors . ^^^ By contrast , all three Ca2+ / calmodulin inhibitors reduced the NE stimulated cAMP and cGMP responses . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Studies described in this report show that vasopressin acts to potentiate norepinephrine ( NE ) induced cyclic adenosine monophosphate ( cAMP ) accumulation in the hippocampus by a calcium dependent mechanism . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These results demonstrate that chronic lithium treatment induced reduction of norepinephrine ( NE ) stimulated phosphoinositide ( PI ) hydrolysis ( Casebolt and Jope , 1987 ) is not due to changes in the alpha 1 adrenergic receptor and is more sensitive to in vivo lithium treatment than is adrenergic stimulated cAMP accumulation . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Responsiveness of norepinephrine ( NE ) sensitive cyclic AMP ( cAMP ) generating systems was determined in slices from different areas of the rat cerebral cortex in which FeCl 2 solution was injected unilaterally into the sensorimotor cortex to induce epileptic activity . ^^^ In anterior cortical areas of rats in which the appearance of electrographic isolated spikes was dominant either ipsilaterally or contralaterally to the injection site 8 10 days after the injection , the cAMP accumulations elicited by NE and an NE phentolamine combination were greater on the side of dominant spike activity than on the other . ^^^ In anterior cortical areas of rats showing dominant spike activity on either side of the cortex 31 60 days after the injection , the cAMP accumulation elicited by NE was smaller on the dominant side than on the other . ^^^ In anterior cortical areas of rats showing nearly equal spike activity on the two sides 31 60 days after the injection , the cAMP accumulations elicited by NE and an NE phentolamine combination were greater on the side ipsilateral to the injection site than on the other . ^^^ In anterior and posterior cortical areas of rats in which the appearance of spike and wave complexes , as well as isolated spikes , was detected 31 60 days after the injection , the cAMP accumulations elicited by NE and combinations of NE and phentolamine or propranolol were greater on the side ipsilateral to the injection site than on the other . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These results are consistent with the view that NE released from sympathetic nerve terminals in the newborn atria maintains myocardial sensitivity at normal level by acting on beta adrenergic receptors , and that the effect may be partially mediated by a rise in intracellular cAMP concentration . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These experiments examined the influence of estradiol and progesterone given in vivo on norepinephrine ( NE ) regulation of cAMP synthesis in hypothalamic and preoptic area slices in vitro . ^^^ Administration of progesterone to estrogen primed female rats attenuated NE induced slice cAMP accumulation . ^^^ This hormone dependent reduction in NE stimulated cAMP synthesis was observed in slices incubated with TTX and in slices prepared from hypophysectomized rats , suggesting that progesterone effects on NE receptor activation of cAMP generating systems are not secondary to the release of neurotransmitters that inhibit adenylyl cyclase or to changes in pituitary hormone secretion . ^^^ Progesterone suppression of NE induced cAMP formation could be prevented by incubating slices in the presence of a phorbol ester . ^^^ In additional studies , the activity of beta NE receptors was assessed by measuring isoproterenol ( ISO ) stimulated cAMP accumulation in the presence of the phosphodiesterase inhibitor RO 20 1724 , and the activity of alpha 1 receptors was evaluated by measuring phenylephrine ( PHE ) augmentation of the ISO response . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The results indicated that the Qigong group after treatment of 9 weeks had more cases with normal sympathetico adrenomedullary functions than it had before the treatment , and that their urinary CA , E , NE decreased , MHPG SO 4 increased , plasma cAMP and cGMP got down , but cAMP / cGMP ratio got up . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Local infusion of the beta agonists norepinephrine ( NE ) and isoproterenol produced rapid ( 3 min ) and marked ( three to sevenfold ) dose dependent increases in extracellular cAMP , which were potentiated by the phosphodiesterase inhibitor rolipram , and blocked by the beta antagonist timolol . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The correlations between actual blood volume ( BV ) , blood pressure ( BP ) , heart rate , and plasma levels of renin activity ( PRA ) , serum aldosterone ( ALD ) , antidiuretic hormone ( ADH ) , epinephrine ( E ) , norepinephrine ( NE ) , atrial natriuretic factor ( ANF ) , cGMP , and cAMP were investigated in 10 stable patients during HD . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Unlike ISO and NE , the membrane permeant cyclic adenosine 3 ' , 5 ' monophosphate ( cAMP ) analogue dibutyryl cAMP ( dbcAMP ) , and the potent adenylate cyclase activator forskolin did not enhance but even inhibited DNA synthesis and Ig secretion stimulated by LPS and Kp MPG . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
On recovery day 14 concentrations of E , NE , BE , STH , AC , cAMP , cGMP , FFA as well as E and dopamine excretion remained elevated while concentrations of T 3 , PL , FT , LT , testosterone PG A + E , PG 2 and excretion of MN , HVA , VMA , MN : E remained decreased , while other parameters returned to the normal . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Vasoactive intestinal peptide ( VIP ) increases cellular cAMP levels in human thyroid cultures and its effect is additive to increases produced by norepinephrine ( NE ) and isoproterenol ( ISO ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
However , NE and carbachol inhibited TSH stimulated cAMP production in both follicles and cells . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The cellular cAMP content in arterial strips after the stimulation with NE was significantly less in the SHR than in the WKY , whereas the cAMP contents were similar in arterial strips from both strains which were stimulated with forskolin . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE induced cAMP production was comparable to that evoked by NE as a beta agonist , and the accumulation of [ 3H ] inositol 1 , 4 , 5 trisphosphate ( IP 3 ) evoked by NE was comparable to that elicited by NE as an alpha agonist . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The goal of the present studies was to determine if the cAMP second messenger system might also be a component of the mechanism responsible for this NE modulatory action on GABA mediated inhibition . ^^^ In summary , these results indicate that a membrane permeant analog of cAMP , a phosphodiesterase inhibitor and an agent which directly activates adenyl cyclase can mimic the previously observed GABA potentiating actions of NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The present experiments examined the effects of progesterone on adrenergic receptor coupling to adenylate cyclase in hypothalamic and preoptic area slices by monitoring norepinephrine ( NE ) stimulated increases in cAMP accumulation . ^^^ Progesterone treatment of estrogen primed rats decreased NE induced slice cAMP accumulation . ^^^ Neither generalized increases in phosphodiesterase activity nor decreases in the catalytic activity of adenylate cyclase could account for the reduced ability of NE to stimulate cAMP accumulation in hypothalamic slices . ^^^ Likewise , NE stimulated cAMP accumulation was completely antagonized only by a combination of both beta ( propranolol ) and alpha 2 ( yohimbine ) antagonists . ^^^ The data suggest that in slices from estrogen plus progesterone treated rats , alpha 2 receptors contribute significantly to NE stimulation of cAMP accumulation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Namely , NE , EP , or cAMP showed positive inotropic action but slight chronotropic action on cultured ventricle cells . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) stimulated the accumulation of cAMP in embryonic rat cerebral cortex in dissociated cell culture . ^^^ After exposure to NE for 10 min , the intracellular cAMP content of these cultures went from 22 + / 12 to 202 + / 75 pmol / mg protein . ^^^ Using selective culturing techniques , evidence was obtained supporting the hypothesis that NE stimulated production of cAMP is a property associated with the glial rather than the neuronal component of these cultures . ^^^ In response to NE stimulation of glia , in particular astrocytes , cAMP or its metabolites may accumulate at high enough concentrations in the extracellular space in cerebral cortex to affect neuronal function , possibly via adenosine receptors . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We examined density ( Bmax ) , affinity ( Kd ) , and norepinephrine ( NE ) stimulated cyclic adenosine monophosphate ( cAMP ) generation of the beta adrenergic receptor on lymphocytes in patients with dementia of the Alzheimer type ( DAT , n = 13 ) , in normal aged controls ( AGED , n = 27 ) , and in young controls ( YOUNG , n = 21 ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The distribution of norepinephrine ( NE ) , cyclic AMP ( cAMP ) and cyclic GMP ( cGMP ) and the activities of related enzymes in the atrioventricular ( A 5 ) conducting tissue of the bovine heart were examined . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Vinpocetine , 8 MeOMeMIX and M & B 22948 at concentrations ( 30 and 100 microM ) which inhibit CaM PDE greater than 60 % increased cGMP but not cAMP levels in l norepinephrine ( NE ) preincubated rabbit aortic slices . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The particulate fraction from ischaemic brain accumulated considerably more cAMP and responded more effectively to stimulation by noradrenaline ( NE ) , histamine ( Hi ) and adenosine than that prepared from normoxic controls . ^^^ The activation of cAMP production by NE and Hi , but not 2 chloradenosine ( 2 CA ) , was totally dependent on the presence of endogenous adenosine and calcium . ^^^ The synergism of 2 CA with NE or Hi on cAMP accumulation was observed . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The evidence supporting noradrenergic action on glia consists primarily of findings that beta adrenoceptors , norepinephrine ( NE ) stimulated cyclic AMP ( cAMP ) responses and glycogen are localized preferentially in glial cells and that beta receptor density and glycogen hydrolysis are under the control of neuronally released NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This study explored an effector mechanism associated with the arginine vasopressin ( AVP ) recognition site in the hippocampus , namely , potentiation of norepinephrine ( NE ) induced cAMP accumulation in the surviving hippocampal slice . ^^^ The biochemical mechanisms that underlie the AVP potentiation were investigated as follows : First , the actions of AVP upon NE induced accumulation of cAMP in hippocampal slices from rat brain were specific to AVP and not shared by other closely related peptides , namely , oxytocin and AVP 4 9 . ^^^ Second , the AVP induced neuromodulation involved beta adrenergic receptors , with AVP having no effect on cAMP levels in the absence of NE . ^^^ Third , the potentiation by AVP was biphasic , with lower AVP concentrations potentiating NE induced cAMP accumulation , while higher concentrations did not potentiate . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE decreases AVP stimulated cAMP generation from 190 + / 11 to 117 + / 10 fm / micrograms , P less than 0 . 001 , N = 6 . ^^^ The specific alpha 2 antagonist , yohimbine , but not the alpha 1 antagonist , prazosin , prevents the NE induced decrease as AVP stimulated cAMP is restored to 187 + / 19 fm / micrograms . ^^^ After pretreatment with PT ( 1 1000 ng / ml ) , AVP stimulated cAMP was not inhibited by NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
PMA simulated the effects of NE and phenylephrine on 3H 5HT efflux , while cAMP had no effect . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This supports the hypothesis that NE and isoproterenol augment features of LTP by stimulating adenosine 3 ' , 5 ' cyclic monophosphate ( cAMP ) production and that cAMP plays a modulatory role in the induction of LTP . 6 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Treatment of intact pinealocytes with H 7 for 0 240 min reduced the effects of subsequent norepinephrine ( NE ) stimulation of cAMP and cGMP accumulation by at least 25 % . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The inhibitory effects of norepinephrine ( NE ) on the cyclic adenosine 3 ' , 5 ' monophosphate ( cAMP ) response to prostaglandin E 1 ( PGE 1 ) , a measure of alpha 2 adrenergic receptor function , have been compared in platelets from drug free schizophrenic patients , depressive patients , and normal controls . ^^^ The absolute value of the inhibition by NE of the cAMP response to PGE 1 was smaller in platelets from schizophrenic and depressive patients than in controls . ^^^ Effects of NE on cAMP production did not discriminate between actively ill and remitted patients with either schizophrenia or depression . ^^^ Platelet alpha 2 receptor sensitivity , as measured by the effects of NE on cAMP production , does not appear to be altered in these psychiatric disorders . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE , isoproterenol ( ISO ) and epinephrine ( E ) stimulate cyclic AMP ( cAMP ) production with equal potency . ^^^ NE stimulation of cAMP was totally blocked by propranolol ( beta antagonist ) but only partially blocked by practolol ( a beta 1 preferential antagonist ) indicating a mixed population of beta 1 and beta 2 receptor subtypes . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Perfusion of probes with NE ( 10 ( 5 ) to 10 ( 3 ) M ) led to dose dependent increases in cAMP efflux . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Circadian rhythms in physiological processes and behaviors were compared with hypothalamic circadian rhythms in norepinephrine ( NE ) metabolites , adrenergic transmitter receptors , cAMP , cGMP and suprachiasmatic nucleus ( SCN ) arginine vasopressin ( AVP ) in a single population of rats under D : D conditions . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We found here that , already during the early growth phase , the cultures ( essentially precursor cells and preadipocytes at this stage ) show increased cyclic AMP ( cAMP ) levels when acutely stimulated with norepinephrine ( NE ) . ^^^ This effect was reproduced by selective activation of adenylate cyclase with cholera toxin , suggesting that the NE effect was exerted through an increase in cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Both types of treatment did not decrease the increase in cyclic AMP ( cAMP ) content of these cells in response to a 10 min exposure to 10 ( 5 ) M NE . ^^^ In contrast to the lack of effect of the tricyclic antidepressants pretreatment of the cultures with 10 ( 5 ) M NE resulted in total inhibition of the cAMP response after 2 h , with a 50 % decrease occurring in about 45 min . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Cyclic 3 ' , 5 ' adenosine monophosphate ( cAMP ) generation by neuronal activity and norepinephrine ( NE ) was studied in rat hippocampal slices . ^^^ NE ( 50 microM ) also caused a 3 fold rise in cAMP in whole slices and this stimulation was not altered by NE depletion . ^^^ These results are consistent with our previous data suggesting that cAMP production is involved in the expression of long term potentiation and NE induced long lasting potentiation in the dentate gyrus . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Plasma glucose concentration , adrenal norepinephrine ( NE ) and epinephrine ( E ) content , spleen NE content , and myocardial NE and cAMP content were analyzed in fed rats given saline or endotoxin ( ETOX , 16 . 7 mg / kg ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Despite its in vitro and in vivo inhibitory activity on NE uptake , 21 repeated daily injections of maprotiline ( 20 mg / kg i . p . ) neither attenuated the norepinephrine stimulated cAMP accumulation nor reduced the number of beta adrenergic recognition sites in rat frontal cortex . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We have examined the interactions , in eliciting cAMP accumulation , between vasoactive intestinal polypeptide ( VIP ) and the three monoamines norepinephrine ( NE ) , histamine ( HIS ) , and serotonin ( 5 hydroxytryptamine , 5 HT ) in mouse cerebral cortical slices . ^^^ We have observed that NE and HIS , but not 5 HT , act synergistically with VIP to increase cAMP levels . ^^^ The rank order of potency of several adrenergic agonists in potentiating the effect of VIP on cAMP levels is the following : epinephrine greater than NE greater than phenylephrine much greater than clonidine , with EC 50 of 2 . 2 , 5 , and 10 microM , respectively ( clonidine being only marginally effective ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Restraint stress reduces the cyclic AMP ( cAMP ) response to norepinephrine ( NE ) in slices of the rat cerebral cortex and hypothalamus . ^^^ Analysis of the NE cAMP dose response curve indicates that the stress reduces the maximum cAMP response to NE but does not increase the EC 50 value of NE . ^^^ An increased release of brain NE may also be involved as repeated administration of the NE reuptake inhibiting antidepressant , desmethylimipamine , reduces the function of non beta as well as beta adrenergic receptors as evidenced by reductions of both the NE and isoproterenol cAMP responses . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These results indicate that VIP ( 10 ( 10 ) M ) selectively inhibits the Ca release activated by NE , and high concentrations ( over 10 ( 9 ) M ) would expectedly increase the Ca extrusion from cells following increase in the levels of cAMP . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
With the injection of other neuroactive substances , values ranging from 160 pmol cAMP / mg protein for norepinephrine ( NE ) , to 15 pmol cAMP / mg protein for gamma amino butyric acid ( GABA ) were observed . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Properties of the norepinephrine ( NE ) stimulated , cAMP generating system were studied in rat hippocampal slices . ^^^ NE but not other putative neurotransmitters , caused a 3 4 fold rise in cAMP levels in the slices . ^^^ The latency to the NE stimulation of cAMP formation was about 20 sec but maximal stimulation was reached only after 5 10 min of incubation . ^^^ NE caused a 12 15 fold rise in cAMP levels in KA treated HPC . ^^^ The beta 1 antagonist , practolol , and the beta 2 antagonist , H35 / 25 , were about equipotent in blocking the NE stimulated cAMP formation in normal HPC . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Pretreatment of the cells with cis DDP significantly potentiated cAMP accumulation in response to NE alone and to cis DDP plus NE could be prevented to a large extent by propranolol ; in cis DDP treated cells the propranolol protection was more effective , both in the absence and the presence of IBMX . ^^^ The results indicate that the cis DDP stimulated cAMP response to NE is mediated via an interaction with beta adrenergic receptors . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The NE stimulated accumulation of 3H inositol phosphates in astrocytes was inhibited by the cyclic nucleotide phosphodiesterase inhibitor isobutylmethylxanthine as well as by the cAMP analog dibutyryl cAMP . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Indomethacin ( 100 microM ) , an inhibitor of phospholipid and arachidonic acid metabolism , was able to inhibit the stimulatory effect of NE ( 84 % ) , and to a lesser extent of TSH ( 63 % ) and cholera toxin , had minor effect on forskolin ( 24 % ) , IBMX ( 16 % ) and Graves ' IgG ( 8 % ) , and no effect on 8 bromo cAMP . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Major differences were observed in the modes of action of the two compounds : ( 1 ) the time course of relaxation to FOR was slower than that to NP ( 15 min vs . 3 min ) even though the cAMP dependent protein kinase activity ratio was maximally elevated at 3 min after the addition of FOR ; ( 2 ) FOR and dibutyryl cAMP prevented contraction to both norepinephrine ( NE ) and KCl whereas NP and 8 bromo guanosine 3 ' , 5 ' monophosphate ( 8 Br cGMP ) were more effective in preventing contraction to NE than to KCl . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The percent norepinephrine ( NE ) inhibition of PGE 1 stimulated cAMP production was similar in patients and controls . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
SCGx resulted in a decrease of median eminence norepinephrine ( NE ) content 16 h after surgery and in an increase of medio basal hypothalamic cAMP synthesis and receptor occupancy . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) , dopamine ( DA ) , serotonin ( 5 HT ) , acetylcholine ( ACh ) , cyclic AMP ( cAMP ) and cyclic GMP ( cGMP ) contents in dissected brain regions were measured after MB exposure . ^^^ MB induced changes in NE contents lasted for at least 24 hr after the cessation of exposure . cAMP was increased and cGMP was reduced by MB exposure . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In CCT , 10 ( 5 ) M NE reduced by 84 % the cAMP generation induced by 10 ( 10 ) M AVP ( 8 . 8 + / 2 . 0 vs . 54 . 2 + / 3 . 5 fmoles / mm , N = 3 ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effects of TSH and NE on the cAMP content of incubated mouse thyroids were also studied . ^^^ In contrast , NE ( 6 10 10 ( 5 ) M ) produced a transient but significant increase in cAMP only within the first 5 min . ^^^ Unlike the action of NE on organification , this short term stimulatory effect on cAMP production was mediated by a beta adrenergic receptor , since it was blocked by l propranolol but not by phentolamine . ^^^ The following conclusions were reached : 1 ) stimulation of iodide organification and thyroid hormone release involves different sensitivity thresholds for TSH and NE ; 2 ) TSH stimulation of iodide organification , hormone release , and cAMP formation is not exerted through adrenergic receptors ; 3 ) NE stimulates organification and inhibits TSH stimulated T 4 release through alpha adrenergic receptors , but stimulates cAMP production through beta receptors ; and 4 ) cAMP may not be the mediator of all TSH actions on the thyroid . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Phosphodiesterase inhibitors or cyclic AMP ( cAMP ) analogues attenuate the NE induced suppression . ^^^ These results suggest that the NE induced suppression is mediated by adenylate cyclase inhibition and a resultant decrease of intracellular cAMP content . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
An analog of cyclic adenosine monophosphate ( cAMP ) , 8 bromo cAMP , mimicked the effect of NE . ^^^ It is suggested that NE activates a Na K ATPase , that this effect might be mediated by cAMP , and that these interrelations may underly the physiological action of NE in the brain . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The role of the cardiac cyclic nucleotides , adenosine 3 ' , 5 ' monophosphate ( cAMP ) and guanosine 3 ' , 5 ' monophosphate ( cGMP ) , and norepinephrine ( NE ) in cardiac responses to stimulation of the left ansa subclavia were studied in anesthetized open chest dogs . ^^^ In three groups of dogs undergoing stimulation for 6 min with impulse frequencies of 4 , 10 , or 20 Hz and 5 5 , left ventricular levels of cAMP , cGMP , and NE were determined at the end of the stimulation period and compared to control dogs . ^^^ The rate of left ventricular pressure rise ( LV dP / dt ) was found to be linearly related to the increase in myocardial cAMP ( P less than 0 . 01 ) rather than to NE levels found after stimulation . ^^^ Propranolol administered before ansa subclavia stimulation caused significant decreases in both cAMP and LV dP / dt , whereas the muscarinic agonist carbachol , caused increases in cGMP and NE and a decrease in LV dP / dt accompanied by a nonsignificant decline in cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Taken together , these results suggest that NE may interact with alpha adrenergic receptors to decrease pyramidal cell excitability , and the beta adrenergic receptors to increase pyramidal cell excitability ; the beta effect may involve cAMP . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Chronic footshock stress in rats produces a persistent reaction in the sensitivity of the norepinephrine ( NE ) cAMP generating system in the cerebral cortex , an effect similar to that reported after chronic antidepressant treatment . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This study has shown , for the first time , that the change in plasma adenosine 3 ' , 5 ' cyclic monophosphate ( cAMP ) , from accommodation or trough levels , correlates highly in both progressive and continuous work stress experiments with epinephrine ( E ) at 0 . 990 and 0 . 898 , and norepinephrine ( NE ) at 0 . 954 and 0 . 927 , respectively , in men subjected to the same relative , rather than absolute , stress levels . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Dibutyryl cAMP ( 0 . 1 mM ) mimicked the effect of NE on pineal [ 14C ] testosterone metabolism ; it also mimicked the NE induced inhibition of [ 14C ] progesterone reduction to 5 alpha pregnanedione and 3 alpha hydroxy 5 alpha pregnan 20 one by rat pineal gland explants . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Many experimental findings suggest that the administration of exogenous Norepinephrine ( NE ) , in cerebral cortical slices surviving in vitro , increases cyclic AMP ( cAMP ) levels , although the NE receptor turns out to be different for the different animal species . ^^^ In this report we studied the interaction of NE on the cAMP system of human cerebral tissue slices , normal and tumoral . ^^^ In the normal slices , NE increases cAMP levels in a dose dependent manner , probably through beta receptors ; in fact , Propranolol counteracts this effect . ^^^ The contemporaneous administration of NE and A produces an effect on cAMP levels greater than that displayed by each drug alone . ^^^ Probably the enhanced cAMP increase is due to the endogenous release , evoked by NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) is unable to modify cAMP concentration in the same preparation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
However , thyroid cAMP was not changed by ACh , suggesting that ACh , like NE , exerted its effects at a step beyond cAMP production . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Analysis of myocardial tissue obtained from the infusion sites showed that cAMP was increased when the NE / Ca2+ VT ensued and that choline esters in the absence of atropine prevent such accumulation of cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The urinary concentrations of cAMP , cGMP , E and NE were monitored daily . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Physiologic responsiveness was determined by measuring the response of cyclic adenosine 3 ' 5 ' monophosphate ( cAMP ) to prostaglandin E 1 ( PGE 1 ) , and the inhibition of the PGE 1 stimulated cAMP response by NE in intact platelets . ^^^ Baseline platelet cAMP production and plasma NE levels were significantly decreased after chronic clorgyline treatment . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Platelet alpha 2 adrenergic receptor number as measured by tritiated dihydroergocriptine ( 3H DHE ) binding is increased in depressed patients compared to controls , while cyclic 3 ' 5 ' adenosine monophosphate ( cAMP ) production in response to prostaglandin E 1 ( PGE 1 ) and norepinephrine ( NE ) inhibition of PGE 1 stimulated cAMP production are reduced in the platelets of depressed patients . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In rats treated with diethyldithiocarbamate to block norepinephrine ( NE ) synthesis , the concentration of the nucleotide decreased and remained at steady levels indicating that cyclic variations of cAMP in the hypothalamus are related to the endogenous release of NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
However the Vmax of the cAMP ( cyclic AMP ) generating system stimulated by NE is reduced suggesting that similarly to other antidepressants bupropion down regulates beta adrenergic receptors located in the frontal cortex . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Dibutyryl cAMP , papaverine ( which inhibits cAMP phosphodiesterase and increases cAMP levels ) , bromo cGMP , and sodium nitroprusside ( which stimulates guanylate cyclase and increases cGMP levels ) inhibited in a concentration dependent manner the biphasic aortic contractions induced by NE in calcium free medium . ^^^ The present findings indicate that the phasic and tonic components of NE induced aortic contractions in calcium free medium are subject to similar intracellular regulatory mechanisms by cyclic nucleotides at a step subsequent to alpha adrenoreceptor occupancy by NE , since dibutyryl cAMP and bromo cGMP inhibited the tonic component of NE induced contraction noncompetitively . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
These results indicate that FK 33 824 induced hyperthermia is not mediated by PG , NE and cAMP , but rather that mu receptors are involved in the induction of hyperthermia by FK 33 824 . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Fifty percent inhibition of both NE induced contractures and NE stimulated 42K effluxes occurred at levels of cAMP that were 1 . 4 times basal , and 90 % inhibition of both processes was associated with a two to threefold increase in cAMP content . ^^^ In sharp contrast , the level of tissue cAMP associated with inhibition of KCl contractures was 6 10 times higher than that associated with inhibition of NE induced contractures . ^^^ We postulate that cAMP dependent regulation of membrane fluxes stimulated by receptor occupancy represents a primary mechanism for relaxation of a NE contracture , whereas the processes that regulate depolarization dependent channels and the phosphorylation of myosin light chain kinase occur at much higher cAMP content and apparently function in a secondary capacity . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In rats receiving ( ) deprenyl ( 1 mumol / kg , s . c . ) twice daily for 3 weeks , the Bmax of imipramine binding sites located in crude synaptic membranes prepared from frontal cortex increases while the NE stimulation of cAMP accumulation in minces prepared from frontal cortex is attenuated . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
To examine the functional responsiveness of the platelet alpha 2 adrenergic receptor , basal cyclic adenosine 3 ' , 5 ' monophosphate ( cAMP ) production , prostaglandin E 1 ( PGE 1 ) stimulation of cAMP production , and norepinephrine ( NE ) inhibition of PGE 1 stimulated cAMP production were measured in 23 depressed patients and 53 control subjects . ^^^ Both the PGE 1 stimulated cAMP response and the inhibition of this response by NE were significantly reduced in the depressed patients compared to the control subjects . ^^^ Plasma NE concentrations were neither significantly different in the depressed patients than in the controls nor correlated with any of the measures of cAMP responsiveness . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
It was manifested by a dose dependent NE inhibition of the PGE 2 stimulated formation of cAMP , which was partially restored by phentolamine . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Sodium nitroprusside ( nitroprusside ) and NE elevated the cGMP and cAMP , respectively , in both HiOxSa and hypoxia . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Lack of antagonism of naloxone on pyrogen , arachidonic acid , PGE 2 , dibutyryl cAMP and NE induced hyperthermia indicates that endogenous opioid peptides are not likely to be central mediators of the hyperthermia induced by these agents . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This experimental paradigm was also used to measure the efflux of cAMP following a brief ( 10 min ) exposure of cells to NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) produces a greater stimulation of cAMP levels than does the beta adrenergic agonist , isoproterenol ( ISO ) , and the NE response is inhibited by both the beta adrenergic antagonist , propranolol , and the alpha adrenergic antagonist , phentolamine . ^^^ Hence , NE stimulation of cAMP levels in striatal slices appears to involve a synergistic interaction between alpha and beta adrenergic receptors . alpha Receptors also potentiate adenosine stimulation of cAMP levels in striatal slices . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Recently , increased brain and spinal fluid ( CSF ) norepinephrine ( NE ) , and a decreased cAMP response to prostaglandin E 1 ( PgE 1 ) stimulation of platelet NE sensitive adenylcyclase were observed in some schizophrenic patients . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Maximum active tension of noninfarcted area of the isolated perfused hearts was markedly reduced 6 hr , 2 days , and 5 days after ligation , associated with significant decreases in norepinephrine ( NE ) and cAMP contents , but with no changes in adenylate cyclase ( AC ) and cAMP phosphodiesterase ( PDE ) activities . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effect of human leukocyte elastase ( hLE ) , a nicking enzyme , on standard hCG also was examined in the cAMP assay and on receptor binding . ^^^ Treatment of standard hCG with hLE increased the cAMP response about two fold . ^^^ Dose dependent displacement of bovine [ 125I ] TSH by standard hCG and hLE increased the cAMP response about two fold . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Generation of cAMP by brown adipocytes in response to norepinephrine ( NE ) and CL 316 , 243 was measured by RIA . ^^^ In BAT , beta 3 AR can account for as much as 50 % of the maximal cAMP response to NE , whereas in WAT these receptors probably account for all the effect of NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This decrease in NE uptake occurs coincident with increases in the density of postsynaptic alpha 1 and beta adrenergic receptors and also NE stimulated accumulation of 3 ' , 5 ' cyclic adenosine monophosphate ( cAMP ) . ^^^ Peaks in the development of alpha 1 receptors ( PND 10 ) and beta receptors ( PND 20 ) and NE stimulated cAMP accumulation ( PND 15 ) were also followed by decreases to adult levels . ^^^ At each day following 6 OHDA , synaptosomal uptake of [ 3H ] NE was reduced by two thirds compared with control values . alpha 1 and beta adrenergic receptor binding are uniformly increased along with a parallel increase in NE stimulated accumulation of cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) regulates melatonin production and many other aspects of pineal function through actions involving cAMP . ^^^ In the present study the effects of NE on the phosphorylation of the cAMP response element binding protein ( CREB ) were studied to determine whether CREB phosphorylation might be involved in cAMP signal transduction in this tissue . ^^^ Pharmacological studies show that NE stimulated CREB phosphorylation is mediated primarily through beta 1 adrenergic receptor stimulated increases in cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Agents that elevate intracellular cyclic AMP ( cAMP ) have been found to enhance the synaptic discharge of norepinephrine ( NE ) from sympathetic nerve terminals in the rabbit iris ciliary body and other peripheral tissues . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) and epinephrine ( EPI ) were equally potent in increasing cAMP accumulation , consistent with a primarily beta 1 response , although both beta 1 and beta 2 components of the response could be isolated using selective agonists ( NE and zinterol ) , and antagonists ( CGP 20712A and ICI 118 , 551 ) . ^^^ Surprisingly , after DEX treatment , NE and EPI were still equally potent in activating cAMP accumulation , although responses to the beta 2 selective agonist , zinterol , were increased . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
DA , norepinephrine ( NE ) and isoproterenol ( Iso ) increased the level of progesterone ( P 4 ) and cAMP in the conditioned medium . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In ovariectomized white rats : ( 1 ) The amount of NE in the hypothalamus increased , and NE , 5 HT , DA , plasma cAMP , ACTH , serum FT 3 , beta EP in pituitary gland markedly lowered in the treatment group . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
DA , norepinephrine ( NE ) and isoproterenol ( Iso ) increased the levels of progesterone ( P 4 ) and cAMP in the media . ^^^ The effect of NE and Iso on P 4 and cAMP levels was inhibited by propranolol ( Pro ; beta blocker ) , while the increase of P 4 and cAMP levels caused by DA or D 1 agonists was suppressed by bulbocapnine ( Bul ; D 1 antagonists ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
After the treatment , examination of the 24 hour urine showed significant increases in its content of norepinephrine ( NE ) , dopamine ( DA ) , 3 4 dihydroxy phenylacetic acid ( DOPAC ) , cyclic adenosine monophosphate ( cAMP ) , and creatinine ( Cr ) . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Application of dibutyryl cAMP ( dbcAMP ) , other cAMP analogs , or forskolin , to LC cultures resulted in a significant increase in NE uptake which was associated with up to a 4 fold increase in the number of TH immunoreactive cells ( TH+ ) . dbcAMP treatment caused an increase in the number of TH+ cells in LC cultures by enhancing their survival and / or by upregulating their phenotypic differentiation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Further study showed that NE induced a significant increase in cyclic adenosine monophosphate ( cAMP ) production by trophoblast tissue . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE induced transient intracellular Ca2+ mobilization and an increase of intracellular cAMP and also , as previously described , a short time concentration dependent decrease of BrI secreted by HTG cells . ^^^ When applied together on HTG cells , NPY and NE induced an increase of intracellular cAMP , a discrete sustained increase of basal intracellular Ca2+ , and a long lasting inhibition of secretion . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The intent of this work was to examine the actions of allicin on 1 ) intraocular pressure ( IOP ) in normal and unilaterally sympathectomized ( SX ) rabbits ; 2 ) cAMP accumulation in the rabbit iris ciliary body ( ICB ) and cultured nonpigmented epithelial ( NPE ) ciliary body cells ; and 3 ) 3H norepinephrine ( NE ) release by calculating fractional tritium overflow in response to electrical field stimulation ( EFS , 5 Hz , 12 V / cm ) in isolated , perfused rabbit ICBs . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The changes in blood volume ( BV ) , atrial natriuretic peptide ( ANP ) , plasma renin activity ( PRA ) , aldosterone ( Aldo ) , norepinephrine ( NE ) , epinephrine ( Epi ) , parathyroid hormone ( PTH ) , arginine vasopressin ( AVP ) and the cyclic nucleotides cAMP and cGMP were measured during a fluctuating BV cycle in 15 patients with end stage renal failure maintained on chronic hemodialysis ( HD ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Concentrations of norepinephrine ( NE ) , neuropeptide Y ( NPY ) , and cyclic AMP ( cAMP ) in aqueous humor were elevated . ^^^ Aqueous humor NE and cAMP concentrations increased , while NPY and protein concentrations did not change . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Phenylpropanolamine , [ 1R , 2R ] ( ) norephedrine , [ 1S , 2S ] ( + ) norephedrine , [ 1S , 2R ] ( + ) norpseudoephedrine , [ S ] ( + ) amphetamine , and [ 1R , 2S ] ( ) ephedrine , were compared with the known beta adrenoceptor agonists [ R ] ( ) epinephrine ( EPI ) , [ R ] ( ) norepinephrine ( NE ) , and [ R * , S * ] ( + / ) isoproterenol ( ISO ) for their ability to increase the intracellular concentration of cyclic 3 ' , 5 ' adenosine monophosphate ( cAMP ) in minces of rat heart . ^^^ Of the compounds investigated only NE , EPI , and ISO , as well as , forskolin , which directly stimulates adenylyl cyclase , significantly ( p < 0 . 05 ) increased intracellular levels of cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Further study showed that NE induced a significant increase in cyclic adenosine monophosphate ( cAMP ) production by trophoblast tissue . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The sensitivity to K channel blockers and regulation by norepinephrine ( NE ) , adenosine 3 ' , 5 ' cyclic monophosphate ( cAMP ) , and increases in intracellular Ca concentration ( [ Ca ] ) were studied . ^^^ NE likely reduces K secretion by decreasing intracellular concentration of cAMP . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
An elevated intracellular concentration of cAMP is a strong negative effector of S 14 gene expression , and neither NE nor cAMP action is totally overcome by T 3 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
For either second messenger system the stimulation by NE and the potentiation by CPA appeared to utilize separate mechanisms as evidenced by the fact that the potentiations by CPA were selectively antagonized by a cAMP analogue or by pertussis toxin , whereas the stimulations by NE were essentially unaffected by these agents . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In this study we found that the endogenous catecholamines epinephrine ( EPI ) and norepinephrine ( NE ) , but not dopamine , serotonin , or histamine , all at 10 microM , significantly stimulated intracellular cAMP accumulation , cAMP transport , and extracellular adenosine accumulation in cortical cultures . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
SR 59230A , in contrast to the selective beta 1 and beta 2 adrenoceptor antagonists ( + / ) [ 2 ( 3 carbamoyl 4 hydroxyphenoxy ) ethylamino ] 3 [ 4 ( 1 methyl 4 trifluoromethyl 2 imidazolyl ) phenoxy ] 2 propanol and erythro ( + / ) 1 ( 7 methylindan 4 yloxy ) 3 isopropylaminob utan 2 ol hydrochloride did not counteract the cAMP production induced by ( ) isoprenaline or norepinephrine ( NE ) in rat brain areas rich in beta 1 or beta 2 adrenoceptors , such as frontal cortex and cerebellum . ^^^ Moreover , in proliferating brown fat cells , in which the beta 1 adrenoceptor is the only beta adrenergic subtype coupled to cAMP production , SR 59230A did not modify the production of cAMP induced by NE , whereas CGP 12177 did . ^^^ In confluent brown fat cells , in which the beta 3 adrenoceptor is the functional beta adrenergic subtype coupled to adenylyl cyclase , SR 59230A antagonized the NE induced cAMP accumulation and glycerol release without affecting their basal values , whereas CGP 12177 , which per se stimulated cAMP accumulation and glycerol release , did not change the NE induced increase of either parameter . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
RESULTS : The arteriole constrictor response to NE and the dilator response to hydrogen peroxide , dibutyryl cGMP and cAMP , and papaverine were not different between the dietary groups . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The responses of the brain to the amino acid deficiency that occur after eating imbalanced amino acid diets ( IMB ) have been associated with decreased concentrations of norepinephrine ( NE ) and cAMP in the anterior piriform cortex ( APC ) , an area essential for the initial feeding responses to amino acid deficiency . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Since norepinephrine ( NE ) influences intracellular calcium concentration [ Ca2+ ] 1 and cAMP levels in C cells , we used different adrenergic agonists and antagonists to investigate the effect of NE on [ Ca2+ ] 1 and cAMP accumulation , and on calcitonin secretion in rat MTC 6 23 cells . ^^^ NE stimulated intracellular cAMP accumulation and calcitonin secretion dose dependent , with 10 ( 7 ) mol / l causing maximal stimulation . ^^^ The NE induced increase in cAMP accumulation / calcitonin secretion could be decreased to baseline by equimolar amounts of the beta adrenergic blocker propanolol . ^^^ The beta adrenergic agonist fenoterol proved to be as effective as NE in stimulating cAMP accumulation / calcitonin secretion . ^^^ NE stimulated calcitonin secretion was also completely blocked by the cAMP antagonist RpcAMPs . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Highly significant correlations were obtained between lymphocyte NE and E on the one hand and cAMP in lymphocytes on the other both in the basal state and after stimulation with isoproterenol . ^^^ It is concluded that endogenous lymphocyte NE and E concentrations in healthy subjects reflected basal cAMP production in lymphocytes and lymphocyte subset composition . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Exposure of GT 1 7 cells to E 2 completely blocked the norepinephrine ( NE ) induced elevation of cAMP content . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In the developing rat pineal gland , norepinephrine ( NE ) stimulated cyclic guanosine 3 ' , 5 ' monophosphate ( cGMP ) formation appears 2 weeks later than that of cyclic adenosine 3 ' , 5 ' monophosphate ( cAMP ) formation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We studied the effects of changing beta 1 and beta 2 adrenergic receptor ( AR ) subtype ratios and densities on cyclic AMP ( cAMP ) responses to norepinephrine ( NE ) and epinephrine ( EPI ) in rat C 6 glioma cells . ^^^ We compared the effects of NE and EPI on cAMP accumulation in each condition and observed a predominantly beta 1 pharmacology ( NE > EPI ) under most conditions . ^^^ The ratio of beta 2 / beta 1 density correlated significantly ( p < 0 . 006 ) with the ratio of the potencies of NE and EPI in increasing cAMP formation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Although the stress exposure did not affect the binding properties of either the alpha 1 or beta adrenoceptors , it suppressed cAMP formation stimulated by NE , but not by isoproterenol or forskolin , in the cerebral cortical slices . ^^^ In the presence of prazosin or phorbol ester , the difference in NE stimulated cAMP formation between the control and the stress groups was totally abolished . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Dibutyryl cAMP also mimicked the effect of NE . ^^^ These results demonstrate that NE activates MAPK directly in brown adipocytes and that the effect of NE is not mediated by PMA sensitive protein kinase C or wortmannin sensitive phosphatidylinositol 3 kinase but rather is likely to be dependent on beta receptor mediated increase in cAMP with a minor contribution of alpha receptor mediated signals . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Reverse transcriptase PCR demonstrates that the addition of NE ; selective beta 1 , beta 2 , or beta 3 adrenergic receptor agonists ; or agents increasing cAMP production , such as forskolin , to brown adipocytes stimulates inducible NOS ( iNOS ) messenger RNA , which is present within 4 h after exposure . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In this study , by means of binding assays on cardiac membranes or by evaluating contractility and cAMP levels of isolated rat atria in the presence of IL 2 , we demonstrate that IL 2 may indirectly stimulate beta adrenergic mediated function by triggering the presynaptic release of norepinephrine ( NE ) on isolated rat atria . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Concomitantly , suppression of norepinephrine ( NE ) induced cAMP formation was found in the cerebral cortical slices of the stress exposed rats without changes in adrenergic alpha 1 or beta receptors . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
When expressed in Spodoptera frugiperda Sf 9 cells the alpha2A subtype showed only inhibition of forskolin stimulated cAMP production when activated by norepinephrine ( NE ) , whereas the alpha2B subtype displayed a biphasic dose response curve with inhibition at low concentrations of NE and a potentiation at higher concentrations . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
METHODS AND RESULTS : Norepinephrine ( NE , 10 ( 7 ) mol / L ) increased peak left ventricular systolic pressure ( LVSP ) and cAMP more than twofold compared with controls . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Finally , ( RS ) alpha methyl 4 tetrazolylphenylglyc ine and particularly ( RS ) alpha methyl 4 phosphonophenylglyci ne , appeared to be the most potent and selective antagonists of L AP 4 induced inhibition of forskolin stimulated cAMP production in adult rat striatal slices . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Perfusates were collected before and after NE infusion for the measurement of PGE 2 , 6 keto PGF 1 alpha , TxB 2 , cAMP , and cGMP . ^^^ Production of cAMP did not differ between the four groups before NE infusion , and NE increased cAMP release in the L NMMA group and controls . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
By contrast , c fos expression and intracellular cAMP concentration do not show any significant change after HNE treatments . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In the present work we investigate atrial natriuretic factor ( ANF ) effects on the endogenous content , utilization and turn over of norepinephrine ( NE ) , on tyrosine hydroxilase ( TH ) activity , on cAMP and cGMP levels , and on phosphatidylinositol hydrolysis in rat adrenal medulla in order to assess the possible mechanisms underlying ANF effects on NE metabolism . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE inhibited the action of dibutyryl cAMP ( dbcAMP ) on iodide uptake in a similar manner to TSH , but did not alter the cyclic adenosine monophosphate ( cAMP ) levels increased by TSH . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Forskolin led to activation of MAP kinase comparable with that induced by NE , and a selective inhibitor of cAMP dependent protein kinase , H 8 , attenuated the NE induced activation of MAP kinase . ^^^ These results demonstrate that NE activates MAP kinase directly in GT 1 1 cells , and that the effect of NE is mediated by increase in the cAMP level and by calcium influx , but not by PMA sensitive protein kinase C or Raf 1 kinase . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The UCP gene is under the control of norepinephrine ( NE ) via cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
L Glutamate or agonists of class 2 receptors decreased NE or forskolin dependent increase of cAMP and serotonin N acetyltransferase activities to similar extents . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
CGRP dose dependently increased cAMP accumulation ( EC 50 = 5 . 25 nM ) , inhibited IP 3 production ( EC 50 = 5 . 4 nM ) and induced relaxation ( EC 50 = 10 nM ) in muscle precontracted with norepinephrine ( NE ) ( 10 microM ) . ^^^ CGRP ( 8 37 ) , a CGRP receptor antagonist , reversed the relaxant action of the neuropeptide and inhibited CGRP induced cAMP accumulation in a concentration dependent manner ( IC 50 = 12 . 5 nM ) . 2 ' , 5 ' dideoxyadenosine ( DDA ) , a specific adenylate cyclase inhibitor , significantly reduced the inhibitory actions of CGRP on NE induced contraction and IP 3 production and inhibited CGRP induced cAMP accumulation in a concentration dependent manner ( IC 50 = 6 . 9 nM ) . ^^^ The mechanism of cAMP inhibition of NE induced IP 3 production and contraction is unclear . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This attenuation was associated with lower ( 65 72 % ) uncoupling protein 1 concentrations but no alterations in NE induced cAMP levels or lipolysis . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Using co treatment with IBMX ( 1 mM ) , a cyclic nucleotide phosphodiesterase inhibitor known to increase NE stimulated cAMP and prazosin ( 1 microM ) , an alpha 1 antagonist , the results indicate that a ) 22 : 6n 3 did not act on cyclic nucleotide phosphodiesterase activities to decrease both cAMP ( 64 % ) and cGMP ( 45 % ) levels and b ) the inhibition of cGMP was higher ( 70 % ) in presence of prazosin . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The results suggest that oocytes and somatic cells are linked by a neuroendocrine loop consisting of NE synthesized in oocytes from actively transported DA and cAMP produced by somatic follicular cells in response to NE induced beta adrenoreceptor activation . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NPY ( 100 nM ) can completely block NE induced MT secretion , but the decrease in cAMP efflux is less . ^^^ These data suggest that NE , VIP , and NPY are differently involved in the cAMP and calcium signaling . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Application of 8 bromo derivative analogs of cAMP or cGMP resulted in attenuation of HNE induced increases in mitochondrial calcium , reactive oxygen species , and neuron loss . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
CONCLUTION : 1 ) Hypoxia stress activates the secretion of corticotrophin ( ACTH ) via cAMP ; 2 ) Adrenocotical function of rats decays during chronic hypoxia ; 3 ) Arg and NE regulate the secretion of plasma corticosterone and synthesis of pituitary cAMP at the hypothalamus level ; 4 ) Hypoxia tolerance of the pika was high . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Effect of CRF , AVP and NE on cAMP in cultured rat anterior pituitary cells during hypoxia ] . ^^^ In the study , the effect CRF ( corticotropin releasing factor ) , AVP ( argipressin ) and NE ( norepinephrine ) on cAMP formation in cultured rat anterior pituitary cells in vitro was examined and the effect of cAMP formation between normoxia and hypoxia ( 8 % O 2 ) was compared . ^^^ The concentration of intracellular cAMP was decreased by NE , but the content of extracellular cAMP was no changed . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE also increased the phosphorylation of cAMP response element binding protein ( CREB ) and perilipin ( PL ) , both of which are protein kinase A substrates that are differentially targeted to the nucleus and lipid droplets , respectively . beta ( 1 ) receptor blockade inhibited NE stimulated phosphorylation of CREB but not PL . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Thus inhibition of proteolysis by NE likely involves a beta ( 3 ) adrenergic receptor mediated increase in cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The stimulatory effect of NE was inhibited in the presence of propranolol , atenolol , Rp cAMP , yohimbine , staurosporine , H 7 and U 73122 but not in the presence of ICI 118 , 551 and prazosin . ^^^ Furthermore , the addition of NE , forskolin , 8 Br cAMP or phorbol 12 myristate ( PMA ) stimulated the expression of pOGH ( ANG N 806 / 779 / 53 / +18 ) , a fusion gene containing the putative cAMP responsive element ( CRE , ANG N 806 / 779 ) upstream of the ANG promoter ( ANG N 53 / + 18 ) in OK 95 cells , but had no effect on the expression of fusion genes containing the mutant of the CRE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Such elevations of HNE were sufficient to kill cyclic adenosine monophosphate ( cAMP ) differentiated motor neuron hybrid cells in vitro , and anti oxidants prevented this HNE dependent cell death . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In vitro , differentiation of the LNCaP and PC3M prostatic tumor cell lines to a NE phenotype can be induced by dibutyryl cyclic AMP ( cAMP ) , suggesting that physiological agents that increase intracellular concentrations of cAMP might regulate NE differentiation in vivo . ^^^ Indeed , we demonstrate in this report that LNCaP cells acquire NE characteristics in response to treatment with physiological and pharmacological agents that elevate intracellular cAMP , agents such as epinephrine , isoproterenol , forskolin , and dibutyryl cAMP . ^^^ Together , these results indicate that chronic cAMP mediated signaling is required to block proliferation of prostate tumor cells and to induce NE differentiation . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
When the beta adrenergic antagonist propranolol was added to the stimulation cultures of l PAM TuB spleen cells at a concentration that prevented NE induced cAMP elevation , the NE mediated decrease in TNF alpha mRNA and NE mediated inhibition of CTL generation were reversed . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In preadipocytes , norepinephrine ( NE ) increased cAMP levels but the beta 3 agonists BRL 37344 and CGP 12177 did not ; in contrast , when the cells had differentiated into mature brown adipocytes , a large cAMP response to the beta 3 agonists had emerged and was now double that to NE ( although the affinity of NE had increased 10 fold ) . ^^^ Although beta 1 receptors remained detectable by [ 3H ] CGP 12177 binding in the mature brown adipocytes , the cAMP response to NE ( based on propranolol inhibitory potency ) switched from beta 1 to beta 3 . ^^^ The increases in cAMP levels could adequately explain the increased cell proliferation in NE stimulated preadipocytes and the NE induced UCP 1 gene expression in mature brown adipocytes . ^^^ The distinctive adrenergic profiles for stimulation of proliferation and of differentiation were thus not due to the existence of additional pathways but to a switch in the type of beta receptor mediating the NE response , coordinated with an alteration in the nuclear response to increased cAMP levels . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effect of 24 h pretreatment with epinephrine ( EPI ) or norepinephrine ( NE ) on alpha ( 2 ) and beta adrenoceptor agonist induced , cAMP responses and G ( i 3 ) alpha protein expression was studied in primary cultures of rat superior cervical ganglionic ( SCG ) neurons . ^^^ A 24 h treatment of SCG neurons with EPI or NE induced desensitization of the cAMP response to the beta adrenoceptor agonist isoproterenol . ^^^ Simultaneously , EPI treatment increased the maximal inhibitory cAMP response to the alpha ( 2 ) adrenoceptor agonist UK 14 , 304 and NE was without effect . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Intracellular cAMP levels after NE and ISO treatments were also measured using a radioimmunoassay . ^^^ Both ISO and NE elevated [ cAMP ] in HCSM cells . ^^^ The reduction in ET 1 induced [ Ca2+ ] 1 after NE / ISO treatment appears to be due to elevated cAMP levels via beta receptor activation , suggesting the existence of receptor cross talk . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This rhythm involves the transcriptional regulation of the AANAT by two norepinephrine ( NE ) inducible transcription factors , e . g . the activator pCREB ( phosphorylated Ca2+ / cAMP response element binding protein ) and the inhibitor ICER ( inducible cAMP early repressor ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In these studies , elevation of cAMP is a critical step in NE induced chemotaxis of monocytes . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The NE induced VEGF expression was fully cAMP mediated , an effect which was inhibited by H 89 and thus was dependent on protein kinase A activity . ^^^ The specific inhibitor of Src tyrosine kinases , PP 2 , markedly reduced the stimulation by NE , which demonstrates that a cAMP dependent Src mediated pathway is positively connected to VEGF expression . ^^^ These results demonstrate that adrenergic stimulation , through beta adrenoreceptor / cAMP / protein kinase A signaling , recruits a pathway that branches off from the NE activated Src Erk1 / 2 cascade to enhance transcription of the VEGF gene . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
To determine whether the inhibitory actions of U 50 , 488H against the effects of beta adrenoceptor stimulation was via a cAMP dependent or a cAMP independent pathway , we determined the effects of U 50 , 488H on NE enhanced cAMP production and [ Ca2+ ] 1 oscillations induced by either forskolin , an activator of adenylate cyclase , or Bay K 8644 , a selective L type Ca2+ channel agonist , in the ventricular myocytes . ^^^ In addition , U 50 , 488H also attenuated significantly the NE induced elevation in cAMP in the heart . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Upon binding of L glutamate to the class 2 metabotropic glutamate receptor , norepinephrine ( NE ) dependent formation of cAMP was inhibited , resulting in decreased serotonin N acetyltransferase ( NAT ) activity and melatonin output . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
On this basis , the aim of the present work was to study ANF effects in the rat hypothalamus on the following processes : endogenous content , utilization and turn over of NE ; tyrosine hydroxylase ( TH ) activity ; cAMP and cGMP accumulation and phosphatidylinositol hydrolysis . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
An analysis of pHi responses to bath HCO 3 reduction revealed that DA , SKF 38393 ( a DA 1 agonist ) , and adenosine 3 ' , 5 ' cyclic monophosphate ( cAMP ) inhibited the basolateral Na+ : HCO 3 cotransporter in rabbit and Wistar Kyoto rat ( WKY ) , if its transport stoichiometry was converted to 3 HCO 3 : 1 Na+ by DMEM plus NE incubation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Promoter analysis using deletion constructs of the CT 1 gene indicated that the NE responsive element located between 2174 / 1540 and this region contained the cAMP responsive element ( CRE ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Norepinephrine ( NE ) is the principal neurotransmitter that drives hormone synthesis by activating the cAMP signaling pathway . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Pretreatment of PC 12 cells with a survival promoting agent , 8 ( 4 chlorophenylthio ) cAMP , prevented both the HNE induced JNK activation and apoptosis . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The results suggest that NE depolarizes the BCs through beta adrenoceptor mediated cAMP formation linking it to activation of 1 ( h ) , which is , at least in part , involved in noradrenergic afferent mediated facilitation of GABAergic synaptic activity at BC PC connections in the rat cerebellum . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Additional cell pH measurements suggest that NE stimulates the basolateral Na+ HCO 3 cotransporter which then becomes susceptible to inhibition by cAMP . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The stimulatory effect of NE was blocked by the presence of propranolol ( beta AR blocker ) , atenolol ( beta 1 AR blocker ) , yohimbine ( alpha 2 AR blocker ) , Rp cAMP ( an inhibitor of cAMP dependent protein kinase AI & AII ) and staurosporine ( an inhibitor of protein kinase C ) , but was not blocked by ICI 118 , 551 ( beta 2 AR blocker ) and prazosin ( alpha 1 AR blocker ) . ^^^ Furthermore , the addition of NE , 8 Br cAMP or PMA stimulated the expression of pOGH ( rANG N 806 / 779 / 53 / +18 ) , a fusion gene containing the putative cAMP responsive element ( CRE , ANG N 806 / 779 ) upstream of the ANG promoter ( ANG N 53 / +18 ) in OK cells , but had no effect on the expression of fusion genes containing the mutant of the CRE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In this study , we examined the phosphorylation of an important transcription factor , cAMP response element binding protein ( CREB ) , which has been implicated in long term memory formation ( McLean et al . 1999 ) during NE induced odor preference learning in normal and olfactory bulb 5 HT depleted rat pups . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Incubation of POA and HYP slices with insulin like growth factor 1 ( IGF 1 ) , which signals via a receptor ( IGF IR ) with endogenous tyrosine kinase activity , enhances NE stimulated cAMP accumulation only in tissue derived from ovariectomized , E ( 2 ) primed animals . ^^^ JB 1 , an antagonist for IGF IR , prevents the IGF 1 enhancement of NE stimulated cAMP accumulation in both POA and HYP slices . ^^^ IGF 1 enhances NE stimulated cAMP accumulation via modulation of alpha ( 1 ) adrenoceptor potentiation of adenylyl cyclase . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Since beta adrenoceptor stimulation raises the smooth muscle cell levels of cAMP , an adenylate cyclase stimulator , forskolin ( 10 ( 5 ) M ) was administered prior to NE application . ^^^ We conclude that the calcium response to NE in the isolated rabbit afferent arteriole is mediated by an alpha 1 adrenoceptor . beta Adrenoceptor stimulation and forskolin blunt the increase in [ Ca2+ ] 1 induced by NE stimulation , indicating that cAMP counteracts the NE induced activation of alpha 1 adrenoceptors . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Using ICBs , BRE and norbinaltorphimine ( nor BNI ) , relatively selective kappa opioid receptor agonist and antagonist , respectively , along with pertussis toxin ( PTX ) , were used to evaluate the effect of 1 ) kappa opioid receptors on [ ( 3 ) H ] norepinephrine ( NE ) release from postganglionic sympathetic neurons , and 2 ) cAMP accumulation . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Estrogen priming shifts the balance of postsynaptic NE receptor signaling in the hypothalamus and preoptic area away from beta adrenergic activation of cAMP synthesis toward alpha ( 1 ) adrenergic signaling pathways . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The present demonstration of the induction of NE transdifferentiation in LNCaP cells by increasing concentrations of VIP adds value to previous observations on the role of cAMP in this process , an interesting topic in the comprehension of the molecular changes that are involved in the progression of prostate cancer to androgen independence . . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Superoxide dismutase ( SOD ) , cyclic adenosine monophosphate ( cAMP ) , norepinephrine ( NE ) , dopamine ( DA ) , 5 hydroxytryptamine ( 5 HT ) , myocyte ultrastructural injury and occurrence of arrhythmia were investigated . ^^^ In rabbits treated with DXR , the levels of SOD were elevated while cAMP , NE , DA , 5 HT and the whole blood viscosity were lowered . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
It has been determined that the elevation of LNCaP intracellular cyclic adenosine monophosphate ( cAMP ) levels through the addition of membrane permeable cAMP analogs , phosphodiesterase inhibitors , adenylate cyclase activators , or components of the cAMP signal transduction pathway can induce reversible neuroendocrine ( NE ) differentiation . ^^^ To our knowledge , this is the first report of the use of differential gene expression techniques to analyze gene expression during cAMP induced NE differentiation in LNCaP cells . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The peak cAMP efflux always preceded the elevation of AA NAT gene expression during the 3 h infusion of VIP or NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We have examined whether a qualitative switch occurs in the response of the ribonucleotide reductase ( RNR ) genes to the effect of the physiological cAMP elevating agent norepinephrine ( NE ) during the development of brown adipocytes . ^^^ The NE stimulation of R 2 expression in preadipocytes was mimicked by forskolin and abolished by H 89 , demonstrating mediation via cAMP and protein kinase A ( PKA ) . ^^^ We conclude that adrenergic stimulation of brown adipocytes by NE specifically elevates expression of the RNR subunit R 2 gene in the proliferative stage of brown adipocyte development , the mediating pathway being a cAMP / PKA cascade further involving Src and the MAP kinase Erk1 / 2 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Full D 2 expression is achieved using NE + T ( 3 ) , especially after prolonged T ( 3 ) exposure . beta ( 3 ) Adrenergic agonists mimic the NE action , whereas cAMP analogs do not . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE , NPY , glutamate , and GABA are neurotransmitters possibly controlling pulsatile LHRH release , and NO , cAMP , and ATP may be diffusible substances involved in pulsatile LHRH release without synaptic input . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Naphazoline induced neuroendocrine changes : increases in ANP and cGMP levels , but suppression of NE , 3H NE , and cAMP levels in rabbit eyes . ^^^ The objective of this study was to determine whether naphazoline , an alpha 2 ( alpha 2 ) / imidazoline ( I 1 ) agonist , can alter endogenous levels of atrial natriuretic peptide ( ANP ) and norepinephrine ( NE ) in aqueous humor and cyclic nucleotide ( cAMP , cGMP ) accumulation and NE overflow in the iris ciliary body ( ICB ) of the rabbit eye . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The effect of NE on Per 1 level was dose and receptor subtype dependent , and both cAMP and cGMP induced Per 1 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
AIM : To evaluate norepinephrine ( NE ) and epinephrine ( E ) plasma concentration and lymphocyte beta 2 adrenoceptor dependent cAMP synthesis in primary pulmonary hypertension ( PPH ) patients in comparison with healthy controls . ^^^ Heart failure progressing from 1 2 to 3 NYHA functional classes was accompanied by plasma NE enhance and lymphocyte beta 2 adrenoreceptor activated cAMP production decrease . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Stimulation of beta ARs on microglia by norepinephrine ( NE ) resulted in an increase in the level of intracellular cAMP and the subsequent expression of interleukin 1beta mRNA . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
AIM : To evaluate carvedilol effect on clinical state , norepinephrine ( NE ) and epinephrine ( E ) plasma concentration and lymphocyte beta 2 adrenoreceptor dependent cAMP synthesis in patients with primary pulmonary hypertension ( PPH ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The rat pineal organ is an established model to study signal transduction cascades that are activated by norepinephrine ( NE ) and cause increases in cAMP levels and stimulation of protein kinase A ( PKA ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Forskolin or the cell permeable Br cAMP caused [ Ca ( 2+ ) ] ( 1 ) rise , which were even higher than with NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Here , we investigated in isolated bovine pinealocytes the NE dependent phosphorylation of the transcription factor cyclic adenosine monophosphate ( cAMP ) responsive element binding protein ( CREB ) and compared the effects of NE with those of vasoactive intestinal peptide ( VIP ) and pituitary adenylate cyclase activating polypeptide ( PACAP ) . ^^^ Immunoblots showed that the NE induced immunoreaction was due to phosphorylation of the transcription factor CREB and another protein , presumably the activating transcription factor 1 ( ATF 1 ) . 10 ( 7 ) m isoproterenol ( ISO ) or 10 ( 5 ) m forskolin mimicked the response to NE indicating that NE acts through the beta adrenergic / cAMP pathway . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Long term AT ( 1 ) receptor blockade decreased adrenomedullary AT ( 1 ) receptor binding , AT ( 2 ) receptor binding and AT ( 2 ) receptor protein , rat tyrosine hydroxylase ( TH ) mRNA , norepinephrine ( NE ) content , Fos related antigen 2 ( Fra 2 ) protein , phosphorylated cAMP response element binding protein ( pCREB ) , and ERK 2 . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
The NE induced effect on 2 deoxy D glucose uptake was mediated by adenylyl cyclase and cAMP because responses were inhibited by the adenylyl cyclase inhibitor 2 ' , 5 ' dideoxyadenosine and the protein kinase A inhibitor 4 cyano 3 methylisoquinoline . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Plasma levels of norepinephrine ( NE ) were measured using high performance lipid chromatography , beta adrenergic receptor density ( Bmax ) and the content of cAMP in peripheral lymphocytes were calculated using 3H dihydroalpneolo as ligand and competitive immunoassay , respectively . ^^^ In the cardiogenic death group , plasma levels of NE and epinephrine ( E ) ( 3 . 74 nmol / L + / 0 . 09 nmol / L and 3 . 17 nmol / L + / 1 . 0 nmol / L ) and the contents of peripheral lymphocyte cAMP ( 3 . 64 pmol / mg protein + / 1 . 4 pmol / mg protein ) were significantly increased as compared with the survival group ( 2 . 68 nmol / L + / 0 . 07 nmol / L , 2 . 41 nmol / L + / 0 . 24 nmol / L and 2 . 73 pmol / mg protein + / 0 . 9 pmol / mg protein , respectively , all P < 0 . 01 ) . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In comparison , SB 202474 , the inactive analog , had no effect on NE or ( Bu ) ( 2 ) cAMP stimulated AA NAT activity or protein level . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE differentiating regimens , either cAMP elevation or androgen deprivation , resulted in a reduced endoplasmic reticulum Ca ( 2+ ) store content due to both SERCA 2b Ca ( 2+ ) ATPase and luminal Ca ( 2+ ) binding / storage chaperone calreticulin underexpression , and to a downregulated store operated Ca ( 2+ ) current . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Among the 4 groups , the plasma levels of NE and E had increased the most in the normal morphology group , followed in decreasing order by the concentric remodeling , concentric hypertrophy , and eccentric hypertrophy groups ; the density of lymphocyte beta AR and the content of intralymphocyte cAMP of peripheral blood in the normal morphology , concentric remodeling , and concentric hypertrophy groups increased while they decreased in the eccentric hypertrophy group in patients with essential hypertension . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Whereas treatment with dibutyryl cAMP or ionomycin mimicked the NE induced MKK3 / 6 and p38MAPK phosphorylation , neither dibutyryl cGMP nor 4beta phorbol 12 myristate 13 acetate had an effect . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
We have previously observed that vasoactive intestinal peptide ( VIP ) stimulates cAMP production mainly through VPAC ( 1 ) receptor , inducing NE differentiation in LNCaP cells . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
RESULTS : ( 1 ) Activation of each of three subtypes resulted in an increase of cAMP accumulation in HEK 293 cells in a dose dependent manner , which was inhibited by selective alpha 1 AR antagonist prazosin . ( 2 ) Comparing the pharmacological property , the maximal responses of alpha1A AR to agonists were the most potent , while the sensitivity of alpha 1 AR subtypes to norepinephrine ( NE ) was the highest . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Agents known to enhance TH and DbetaH transcription , phosphorylation or activity ( e . g . , 8 bromo cAMP , forskolin or dexamethasone ) reversed the inhibitory effects of atrazine and simazine on the NE . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Factors such as IL 6 , epinephrine , and forskolin induce NE differentiation in prostate cancer cells ; the mechanisms involve increases in intracellular cAMP , protein kinase A ( PKA ) activation and reduced intracellular calcium levels . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE binds to both alpha 1 and beta adrenergic receptors present on the pinealocyte membrane and initiates adrenergic signal transduction via cyclic adenosine monophosphate ( cAMP ) and cyclic guanosine monophosphate ( cGMP ) generating pathways . ^^^ The NE induced adrenergic signal transduction switches ' on ' melatonin synthesis during the early hours of night by stimulating expression of the rate limiting enzyme of melatonin synthesis , N acetyltransferase ( AA NAT ) via cAMP protein kinase A ( PKA ) cAMP response element binding protein ( CREB ) cAMP response element ( CRE ) pathway as well as by increasing AA NAT activity via cAMP PKA 14 3 3 protein pathway . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
This was accomplished by measuring NE and beta 3 adrenoceptor ( beta 3 AR ) agonist ( BRL 37344 ) induced lipolysis ( glycerol release ) as well as NE induced cAMP accumulation by inguinal , epididymal , and retroperitoneal WAT ( IWAT , EWAT , and RWAT ) in isolated adipocytes of LD and SD housed hamsters . ^^^ NE stimulated adipocyte accumulation of cAMP was greater after 5 wk of SDs for IWAT and EWAT and after 10 wk of SDs for IWAT compared with LDs , with no photoperiod effect for RWAT . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE differentiating regimens , namely , chronic cAMP elevation or androgen deprivation , resulted in generally augmented 1 ( Cl , swell ) and enhanced RVD . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NE is known to markedly elevate pineal cAMP , and here it was found that cAMP agonists elevate MAT2A mRNA levels by increasing MAT2A mRNA synthesis and that drugs that block cAMP activation of cAMP dependent protein kinase block effects of NE . ^^^ Therefore , the NE cAMP dependent increase in pineal MAT activity seems to reflect an increase in MAT 2 protein , which occurs in response to cAMP > protein kinase dependent increased MAT2A expression . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
RESULTS : Under agonist stimulation , alpha1B AR mediated cAMP synthesis in HEK 293 cells , and blockade by PLC PKC or tyrosine kinase did not reduce cAMP accumulation induced by NE . ^^^ Pretreatment with pertussis toxin ( PTX ) had little effect on basal cAMP accumulation as well as norepinephrine ( NE ) stimulated cAMP accumulation . ^^^ The extracellular Ca2+ chelator egtazic acid ( EGTA ) , nonselective Ca2+ channel blocker CdCl 2 and calmodulin ( CaM ) inhibitor W 7 significantly reduced NE induced cAMP accumulation from 1 . 59 % + / 0 . 47 % to 1 . 00 % + / 0 . 31 % , 0 . 78 % + / 0 . 23 % , and 0 . 90 % + / 0 . 40 % , respectively . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
METHODS : A total of 83 identified CHF patients with a baseline and follow up plasma levels of norepinephrine ( NE ) and epinephrine ( E ) , lymphocytes beta AR density ( Bmax ) , and intralymphocyte cAMP content in peripheral blood were followed up . ^^^ Persistence of high NE , E , and cAMP from baseline to follow up were confirmed as risk predicting factors of cardiovascular events . ^^^ Persistence NE above 4 . 0 nmol / L , E above 3 . 5 nmol / L , and the intralymphocyte cAMP content above 3 . 5 pmd 10 mg ( 1 ) 10 pro ( 1 ) from baseline to follow up were significant adverse prognostic predictors . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
In the whole cell patch clamp recording mode 1 ( Cl , swell ) could be activated in response to hypotonicity induced cell swelling in control and NE differentiated ( by incubation in membrane permeable cAMP analogs ) LNCaP cells even following total depletion of intracellular ATP using a cocktail of metabolic inhibitors . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
An increase and sustained activation of adenosine 3 ' , 5 ' cyclic monophosphate ( cAMP ) levels induced NE like differentiation in NSCLC cell lines , and a concomitant increase in the expression of mac25 / IGFBP rP 1 and 25 . 1 was observed during the cAMP regulated differentiation of NCI H 157 cells , suggesting the involvement of these proteins . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Cell culture studies indicate that whereas the NE > cGMP activation of p42 / 44MAPK is rapid and transient , the NE > cAMP activation of p38MAPK is slower and more sustained . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Chronotropic responsiveness to norepinephrine ( NE ) , together with production of cAMP and cGMP , was assessed in isolated atrial preparations from age matched hypertensive and normotensive rats . ^^^ These atria were hyperresponsive to NE at all of the concentrations tested , with elevated production of cAMP . ^^^ NE stimulated cAMP concentration and L type calcium current were also attenuated by adenoviral nNOS , along with the chronotropic responsiveness to NE in hypertensive rat atria . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
Neutrophil products , including neutrophil elastase and antimicrobial peptides such as neutrophil defensins and LL 37 , have been demonstrated to display chemotactic activity towards cells from both innate and adaptive immunity . ^^^
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49913 and P08246	Pubmed	SVM Score :0.0
NA