| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.58828059 |
| To investigate interactions between BYDV MP and the NE , wild type and mutant open reading frame ( ORF 4 ) green fluorescent protein ( GFP ) fusion cistrons were expressed in insect cells . 0.58828059^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In young larvae of M . endothoracicus , AlP and NE activity was highest in the modified bladder wall forming a verrucose mound , and at the site of the opening of the invaginated canal . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We found a significant negative correlation between neutrophil elastase activity and ALP ( r = 0 . 58 ; p < 0 . 01 ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The selectivity that is observed between the proteases and the hexapeptide phosphonates 6o 5 ( Boc AAPV psi [ P = O ( OPh ) O ] AA OMe ) and 6o F ( Boc AAPF psi [ P = O ( OPh ) O ] AA OMe ) parallels that between these enzymes and their substrates : ALP and HLE are selectively inactivated by the ValP containing analogue 6o 5 , while subtilisin ( SUB ) shows a preference for the PheP derivative 6o F . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Antileucoprotease ( ALP ) , a potent inhibitor of human neutrophil elastase ( HNE ) , may be a modulating factor in the pathogenesis of emphysema . ^^^ In vivo efficacy of ALP on HNE induced pulmonary lesions was studied by instillation of either 365 micrograms or 730 micrograms ALP , followed after 1 h by 420 micrograms HNE . ^^^ These results show that ALP inhibits efficiently the development of HNE induced emphysema and , to a lesser extent , haemorrhage . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We have determined the effect of two elastase specific synthetic low molecular weight substrates , L pyroglutamylprolylvaline paranitroanilide and succinyltrialanyl paranitroanilide , together with insoluble elastin fluorescein , on the determination of the neutrophil elastase ( NE ) inhibitory capacity of purified alpha 1 proteinase inhibitor ( alpha 1 PI ) and bronchial antileukoprotease ( ALP ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| A substantial proportion of NE inhibition in secretions could not be accounted for by the amount of alpha 1PI , ALP and alpha 2M present ( median 74 . 8 % , range 43 . 2 97 . 4 , for alpha 1PI deficient sol phase ; median 50 . 0 % , range 0 80 . 8 , for bronchitic sol phase ) . ^^^ Gel filtration of sol phase demonstrated the presence of NE inhibition in the low molecular weight fractions which was markedly sensitive to changes in substrate concentration and ionic strength , in contrast to purified alpha 1PI and ALP . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human lung antileukoprotease ( ALP ) is a potent inhibitor of neutrophil elastase that is locally produced by submucosal bronchial glands and by secretory cells in the bronchiolar epithelium . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Two days after an 4 infusion of norepinephrine ( NE ) ( 4 micrograms . kg 1 . min 1 10 60 min ) in rabbits , patchy myocardial damage in frozen cross sections of right and left papillary muscles was associated with loss of staining for alkaline phosphatase ( ALP ) , an enzyme present in normal capillary endothelium , whereas a regular pattern of staining was observed in control normal muscles . ^^^ Myocardial damage induced by a high dose of NE is therefore associated 48 hr later with loss of ALP staining and endothelial cell disruption and edema , which may impair capillary perfusion and contribute to limited working cardiac performance observed previously . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We have determined the effect of altering assay conditions on the observed neutrophil elastase inhibitory capacity in lung secretions from emphysematous patients with normal serum alpha 1 PI . alpha 1 PI , ALP and alpha 2 macroglobulin were detected in all samples . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The starting material , concentrates , and eluates were assayed for immunoglobulin A ( IgA ) , albumin ( Alb ) , alpha 1 proteinase inhibitor ( alpha 1 PI ) , antileukoprotease ( ALP ) , and total leukocyte elastase inhibitory capacity ( LEIC ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Most of the available histochemical methods and techniques ( azodye , metal salt and indigogenic methods , cryostat , free floating and lyophilized section techniques ) and different modifications of these methods ( different substrate concentrations , pH , temperature , incubation time e . g . ) were applied to study the distribution of acid phosphatase ( AcPB = after Barka and Anderson ; AcPG = after Gomori ) , beta glucuronidase ( beta Glu ) , aryl sulfatase ( AS ) , beta N acetylglucosaminidase ( NAG ) , acid 5 ' nucleotidase ( a 5 Nucl ) , non specific esterase ( NE ) and alkaline phosphatase ( AlP ) in the kidneys of rats of both sexes . ^^^ As most important proved : the incubation of free floating sections cut from `` standard ' ' fixed ( 2 h in formol calcium continued for another 18 22 h in the same fixative plus 0 . 88 M sucrose at 4 degrees C ) kidney slices only for AcPB and NE material fixed after Holt had to be used ; the incubation for AlP and NE at 4 degrees C ; final pH of the incubation medium for AcPB 5 . 5 , AcPG 5 . 0 and NE 6 . 5 ; the use of Fast Garnet GBC Salt as coupler in the NE azo dye reaction . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Compared to the native form of ALP the mutant ALP 242 was less inactivated , whereas ALP 231 was nearly totally resistant towards all reactive oxygen . ( Heinzel Wieland R . et al . , Biomed Biochim Acta 50 : 677 681 ( 1991 ) ) The intratracheal administration of HLE into the lung of Syrian Hamsters induced mild to moderate emphysematous lesions . ^^^ The inhibitory potencies of native ALP and the ALP mutants were determined in this animal model by means of intratracheal instillation of the different molecules one hour prior to the administration of HLE . ^^^ The inhibitory effects of ALP 242 and ALP 231 towards HLE induced emphysema were significantly better than that of the native molecule . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In a human osteosarcoma cell line ( SaOS 2 ) , HNE exhibited an early cytotoxic effect characterized by apoptosis , cytostatic and differentiating effects characterized by slow growth , increase in alkaline phosphatase ( ALP ) , and alpha 5 integrin subunit content with decrease in tumorigenicity . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Studies on the interaction between leukocyte elastase , antileukoproteinase and the plasma proteinase inhibitors alpha 1 proteinase inhibitor and alpha 2 macroglobulin . ^^^ The dominating inhibitor of leukocyte elastase in human respiratory tract secretions is a low molecular mass inhibitor , designated antileukoproteinase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Topographic relation between skin derived antileukoproteinase ( SKALP ) and leukocyte elastase in a case of annular pustular psoriasis . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Demonstration of skin derived antileukoproteinase ( SKALP ) and its target enzyme human leukocyte elastase in squamous cell carcinoma . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Expression of human leukocyte elastase inhibitor , elafin , otherwise known as skin derived antileukoproteinase inhibitor ( SKALP ) , was investigated in normal and abnormal oral tissues using a specific anti SKALP rabbit antiserum . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Skin derived antileukoproteinase ( SKALP ) , also known as elafin , is a potent inhibitor of human leukocyte elastase and proteinase 3 , two PMN derived proteinases implicated in tissue destruction and leukocyte migration . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| As secretory leukoprotease inhibitor ( SLPI ) and elafin / skin derived antileukoproteinase ( SKALP ) are locally produced in the lung and inhibit neutrophil elastase activity , we hypothesized that adenoviral E1A might affect the production of these antiproteases . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Bronchial leukocyte proteinase inhibitor ( BLPI ) is an 11 . 7 kDa , acid stable protein found in mucous secretions , which inhibits neutrophil elastase . ^^^ BLPI , due to its small compact size , can inhibit neutrophil elastase after the enzyme has been complexed with alpha 2 macroglobulin ( A 2 M ) but alpha 1 proteinase inhibitor failed to inactivate A 2 M bound elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Attention also was focused on the bronchial leukocyte proteinase inhibitor ( BLPI ) , which inhibits HNE in the bronchial secretions . ^^^ Studies on BLPI showed that it is a significant inhibitor of HNE and probably plays a more important role in protecting the lung than previously thought . ^^^ In vitro exposure of alpha 1 PI and BLPI to 800 moles of NO 2 per mole of inhibitor resulted in 35 % and 50 % losses of HNE inhibitory activity , respectively . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| At 40 mol O3 / mol protein , BLPI lost 79 % of its activity against HNE and 87 % of its Cat G inhibitory activity . ^^^ Methionine specific modification of BLPI reduced its HNE and Cat G inhibitory activities . ^^^ Two moles of N chlorosuccinimide per mole of BLPI methionine caused an 80 % reduction in activity against Cat G , but only a 40 % reduction in HNE inhibitory activity . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In contrast , most normal subjects and even smokers who do not develop airflow obstruction do not exhibit such airway wall remodelling , increased NE cells , or increased BLP levels . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The hyperplastic neuroendocrine ( NE ) cells contain multiple neuropeptides , including the bombesinlike peptides ( BLP ) , which are likely important in the pathogenesis of the disorder by stimulating proliferation of fibroblasts in a paracrine fashion and the NE cells themselves in an autocrine manner . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In early studies , we determined mechanisms by which NE cell derived peptides such as bombesin like peptide ( BLP ) promote normal fetal lung development . ^^^ We speculate that overproduction of NE cell derived peptides such as BLP may be responsible for a variety of chronic inflammatory disorders . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The role of the antiproteases alpha 1 proteinase inhibitor ( alpha 1PI ) and mucus proteinase inhibitor ( MPI ) in human lung emphysema was investigated by measuring their amount and functional activity against trypsin , leukocyte elastase , and pancreatic elastase in the bronchoalveolar lavage fluid ( BALF ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Oxidation of mucus proteinase inhibitor ( MPI ) transforms Met 73 , the P ' 1 residue of its active center into methionine sulfoxide and lowers its affinity for neutrophil elastase [ Boudier , C . , and Bieth , J . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Combination of MP and 2DG , but not MAC and 2DG , produced a greater NE turnover than 2DG alone . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Low molecular mass heparin ( 5 . 1 kDa ) forms a tight complex with mucus proteinase inhibitor , the physiologic neutrophil elastase inhibitor of the upper respiratory tract . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In the nanomolar enzyme and inhibitor concentration range , 1 mol of mucus proteinase inhibitor ( MPI ) inhibits 1 mol of neutrophil elastase , cathepsin G , trypsin , and chymotrypsin . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We have developed a method for electrotransfer of strongly basic proteins ( lysozyme , pI 11 ; mucus proteinase inhibitor , pI greater than 10 ; bovine pancreas trypsin inhibitor ; pI 10 . 5 ; human leukocyte elastase , pI greater than 9 ) from nondenaturing acid gels ( pH 4 . 5 ) to nitrocellulose sheets . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human mucus proteinase inhibitor is a two domain protein which inactivates bovine trypsin and chymotrypsin , leukocyte elastase and cathepsin G . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| DNA binds neutrophil elastase and mucus proteinase inhibitor and impairs their functional activity . ^^^ DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase Sepharose , inhibitor Sepharose and DNA cellulose . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Influence of low molecular mass heparin on the kinetics of neutrophil elastase inhibition by mucus proteinase inhibitor . ^^^ Commercial low molecular mass heparin accelerates the inhibition of neutrophil elastase by mucus proteinase inhibitor , the predominant antielastase of lung secretions ( Faller , B . , Mly , Y . , Grard , D . , and Bieth , J . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Heparin accelerates the inhibition of neutrophil elastase by mucus proteinase inhibitor ( MPI ) , the physiological antielastase of airways as a result of its binding with the inhibitor [ Faller , B . , Mly , Y . , Grard , D . , & Bieth , J . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Oxidized mucus proteinase inhibitor : a fairly potent neutrophil elastase inhibitor . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Effect of polynucleotides on the inhibition of neutrophil elastase by mucus proteinase inhibitor and alpha 1 proteinase inhibitor . ^^^ We used tRNA and synthetic polynucleotides as models of DNA to study the influence of polynucleotides on the inhibition of neutrophil elastase by its endogenous inhibitors alpha 1 proteinase inhibitor ( alpha 1 PI ) and mucus proteinase inhibitor ( MPI ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| A high performance liquid chromatographic assay of methamphetamine ( MP ) and its related compounds , i . e . ephedrine ( EP ) , norephedrine ( NE ) , p hydroxymethamphetamine ( p HMP ) , p hydroxyamphetamine ( p HAP ) and amphetamine ( AP ) , with peroxyoxalate chemiluminescence detection has been developed . 4 ( N , N Dimethylaminosulphonyl ) 7 fluoro 2 , 1 , 3 benzoxadiazole ( DBD F ) was used as a fluorescent labeling reagent . ^^^ The detection limits ( S / N = 3 ) for the proposed method for MP , AP , EP , NE , p HMP and p HAP were 27 , 100 , 40 , 133 , 25 and 133 fmol on column , respectively . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The partial N terminal amino acid sequence of ESI shows some homology with MPI , especially around the reactive site of MPI for human neutrophil elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| To define doses which effectively suppress reproductive function in NZB / W mice , we implanted groups of NZB / W females with Silastic capsules containing increasing doses of four compounds : norethindrone ( NE ) 0 . 5 5 . 0 mg , norgestrel ( NG ) 1 . 0 7 . 5 mg , medroxyprogesterone ( MP ) 0 . 5 20 . 0 mg , and ethinyl estradiol ( EE ) 0 . 5 5 . 0 mg . ^^^ Based upon these parameters , we determined that effective doses were 5 . 0 mg NE , 7 . 5 mg NG , and 0 . 5 mg EE given as single implants and 10 . 0 mg MP given in two 5 . 0 mg implants . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The highly purified products ( DA 4 O S , NE 3 O S , EPI 3 O S ) were characterized by their melting points ( mp ) , infrared spectra ( IR ) , thin layer chromatography ( TLC ) , high performance liquid chromatography ( HPLC ) , elemental analysis , and 1H nuclear magnetic resonance spectroscopy ( 1H NMR ) . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The cardiovascular actions of single 10 micrograms / kg 4 doses of norepinephrine ( NE ) and isoproteronol ( ISO ) , before and after the administration of a single 30 mg / kg 4 dose of the glucocorticoid methylprednisolone ( MP ) ( sodium succinate ester ) , were compared in adult cats . ^^^ MP treatment significantly reduced the increase in systolic and diastolic pressures caused by NE . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| To examine links between norepinephrine ( NE ) stimulated sodium transport and gluconeogenesis , we perfused isolated rat kidneys with 6 % albumin , containing various combinations of glucose , alanine , pyruvate . and lactate and inhibitors of gluconeogenesis ( 0 . 1 mM mercaptopicolinate , MP ) or glucose metabolism ( 0 . 2 0 . 5 mM 2 deoxyglucose , DG ) . ^^^ In lactate and / or pyruvate perfused kidneys NE decreased fractional water excretion with little increase in free water clearance ; free water formation was lowest in kidneys perfused with DG or MP . ^^^ MP inhibited gluconeogenesis both in the basal state and after NE . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human mucous proteinase inhibitor ( MPI ) is present in bronchial secretions , where it participates in the protection of lung structures against degradation by leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| A marked elevation in MAO activity was seen during MP withdrawal when 5 HT , DA , beta phenylethylamine ( beta PEA ) and norepinephrine ( NE ) were used as substrates . ^^^ The kinetics of MAO showed a significant decrease in Km values , but no significant change in Vmax values during MP withdrawal , despite the presence of NE . ^^^ Inhibition of MAO by MP or its metabolites ( amphetamine , p hydroxyamphetamine and p hydroxymethamphetamine ) increased with the use of the following substrates in the order : DA , 5 HT , NE and beta PEA . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| One hundred and twelve specimens of the common shrew from 21 populations in NE Poland were studied from an area of contact between chromosomal race 2 ( metacentric chromosomes : hi , ko , gm , np ) and race 7 ( ki , hn , gr , mp ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In showing for the first time that MPI and elafin ( and its precursor ) are secreted by the A 549 cell line , this report implicates the type 2 alveolar cell in the defense of the peripheral lung against the neutrophil elastase secreted during inflammation . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Furthermore , recombinant MPI retains full inhibitory activity against chymotrypsin and leukocyte elastase indicating that the protein was folded properly . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In the states of Bihar , Rajasthan , UP , MP , and NE States ( combined ) coverage levels were relatively lower . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| An international collaborative study was conducted of a high performance liquid chromatography ( HPLC ) UV method for the determination of the major ( ephedrine [ EP ] and pseudoephedrine [ PS ] ) and minor ( norephedrine [ NE ] , norpseudoephedrine [ NP ] , methylephedrine [ ME ] , and methylpseudoephedrine [ MP ] ) alkaloids in selected dietary supplements representative of the commercially available products . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We examined the effects of norepinephrine ( NE ) , isoproterenol ( ISO ) and metaproterenol ( MP ) on natural killer ( NK ) cell cytolytic activity and on the protein and mRNA levels of the regulatory factors perforin and granzyme B and interferon gamma ( IFN gamma ) in splenocytes . ^^^ NE , the beta adrenergic agonist ISO , and the beta 2 selective agonist MP all inhibited the protein and mRNA levels of perforin , granzyme B and mRNA levels of IFN gamma . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| To attempt to understand the lack of benefit in these trials , we have examined the effects of MP and two other progestogens , the less androgenic desogestrel ( DG ) and the more androgenic norethisterone ( NE ) , on cardiovascular risk factors against a background of oestrogen therapy . ^^^ DESIGN AND MEASUREMENTS : Thirty four women were treated with conjugated equine oestrogens ( CEE ) 0 . 625 mg daily alone for 12 weeks , followed in random order by each of the three progestogens ( DG 75 microg , MP 10 mg and NE 1 mg daily ) given sequentially for three 12 week cycles while maintaining the same CEE treatment . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We simultaneously recorded SM isometric tension and MP in de endothelised rat aorta rings and examined their submaximal activation by K+ , norepinephrine ( NE ) or phenylephrine ( PHE ) and the influence of methoxyverapamil ( D 600 ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Besides its direct elastolytic activity , NE releases soluble epidermal growth factor receptor ( EGFR ) ligands and initiates EGFR / MEK / ERK signaling to downregulate tropoelastin mRNA in neonatal rat lung fibroblasts ( DiCamillo SJ , Carreras 1 , Panchenko MV , Stone PJ , Nugent MA , Foster JA , and Panchenko MP . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Addition of increasing amounts of Secretory leukocyte protease inhibitor ( SLPI ) to the incubation mixtures resulted in binding of leukocyte elastase to this inhibitor and extinction of free proteolytic activity against both natural and synthetic substrates . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a two domain protein that inhibits a wide range of proteases including chymotrypsin , leukocyte elastase , and trypsin . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Studies were undertaken to evaluate the in vitro properties of recombinant human secretory leukocyte protease inhibitor ( rSLPI ) that had been made in Escherichia coli in an inactive form and refolded , and to determine whether emphysema and bronchial secretory cell metaplasia , induced in hamsters by intratracheal treatment with human neutrophil elastase ( HNE ) , could be amelio rated by prior intratracheal instillation of rSLPI . ^^^ Recombinant human secretory leukocyte protease inhibitor : in vitro properties , and amelioration of human neutrophil elastase induced emphysema and secretory cell metaplasia in the hamster . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Isolation , properties , and complete amino acid sequence of human secretory leukocyte protease inhibitor , a potent inhibitor of leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Neutrophil elastase increases secretory leukocyte protease inhibitor transcript levels in airway epithelial cells . ^^^ In the conducting airway , proteases , particularly neutrophil elastase , are counter balanced by several antiproteases , including secretory leukocyte protease inhibitor ( SLPI ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| PMN were manipulated in the following four ways : ( 1 ) inhibition of oxygen free radicals using manganese superoxide dismutase and catalase ( SOD / CAT ) , ( 2 ) antagonism of PMN elastase using secretory leukocyte protease inhibitor ( SLPI ) , ( 3 ) neutropenia using PMN antisera , and ( 4 ) inhibition of activation and adhesion using interleukin 1 receptor antagonist ( IL 1ra ) and tumor necrosis factor binding protein ( TNFbp ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemistry , showed a human leukocyte elastase dose dependent increase of secretory leukocyte protease inhibitor containing cells and a basal extracellular localization of secretory leukocyte protease inhibitor after incubation with 100 microg / ml human leukocyte elastase . ^^^ The relative amount of secretory leukocyte protease inhibitor mRNA transcripts increased with respect to the human leukocyte elastase concentration . ^^^ Nevertheless , the potential stimulation of secretory leukocyte protease inhibitor secretion by human leukocyte elastase was lower in the more remodelled and inflamed tissue . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The major physiological role of human secretory leukocyte protease inhibitor ( SLPI ) , a low molecular weight inhibitor present in mucus , is the rapid formation of a tight binding inhibitory complex with neutrophil elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| ICI 200 , 355 ( an inhibitor of neutrophil elastase and proteinase 3 ) or secretory leukocyte protease inhibitor ( an inhibitor of elastase but not of proteinase 3 ) abolished IL 8 induced GC degranulation , implicating elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The protease antiprotease balance was studied by measuring immunoreactive anionic trypsin ( irAT ) , cationic trypsin ( irCT ) , complexes between cationic trypsin and alpha 1 protease inhibitor ( irCT alpha 1 PI ) , leukocyte elastase and neutrophil proteinase 4 ( NP 4 ) , as well as the endogenous protease inhibitors , pancreatic secretory trypsin inhibitor ( PSTI ) , alpha 2 macroglobulin ( alpha 2M ) , alpha 1 protease inhibitor ( alpha 1 PI ) , antichymotrypsin ( ACHY ) , and secretory leukocyte protease inhibitor ( SLPI ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a potent inhibitor of human leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Concentrations of alpha ( 1 ) protease inhibitor ( API ) , secretory leukocyte protease inhibitor ( SLPI ) , NE API complex , and myeloperoxidase ( MPO ) were measured by ELISA . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a neutrophil elastase inhibitor which also has antibacterial and anti inflammatory properties . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a potent inhibitor of human leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| This protein had an apparent molecular mass of about 10 kDa on SDS PAGE and the N terminal sequence showed some homology to secretory leukocyte protease inhibitor ( SLPI ) , which is known as the predominant inhibitor of neutrophil elastase and cathepsin G . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a potent inhibitor of human leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a potent inhibitor of human leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Because neutrophil elastase appears to be a key mediator of tissue damage in CF lung disease , anti elastase compounds have also been studied , including alpha 1 protease inhibitor , secretory leukocyte protease inhibitor , and small molecule inhibitors . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Secretory leukocyte protease inhibitor ( SLPI ) is a protein found in various fluids , including parotid secretions , cervical mucus , seminal plasma and ascites , and is a potent inhibitor of human leukocyte elastase activity . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Increased leukocyte elastase activity in mice lacking secretory leukocyte protease inhibitor ( SLPI ) leads to impaired wound healing due to enhanced activity of TGFbeta and perhaps additional mechanisms . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte protease inhibitor ( SLPI ) is a natural NE inhibitor present in amniotic fluid , fetal membranes and cervical mucus . ^^^ Neutrophil elastase and secretory leukocyte protease inhibitor in prelabor rupture of membranes , parturition and intra amniotic infection . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Human oral epithelial cells but not HGF express mRNA and protein of secretory leukocyte protease inhibitor , an inhibitor of HLE and Cat G , and recombinant secretory leukocyte protease inhibitor clearly inhibited the activation of HGF induced by HLE and Cat G but not by PR 3 . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Two of these fragments exhibited high homology to previously characterized human genes : 1 ) secretory leukocyte protease inhibitor , also known as antileukoprotease , an endometrial neutrophil elastase inhibitor with antibacterial and anti inflammatory properties ; and 2 ) syncytin , also known as endogenous retrovirus W envelope protein , a highly fusogenic membrane glycoprotein that induces formation of giant syncytia and is believed to be important in decidual and placental development . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| To counteract the activity of HNE , we have examined the effects of adenoviral gene delivery of the anti elastases elafin , previously demonstrated within human atheroma , and murine secretory leukocyte protease inhibitor ( SLPI ) , a related molecule , on the inflammatory responses of human endothelial cells and macrophages to atherogenic stimuli . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : Secretory leukocyte protease inhibitor ( SLPI ) is a potent inhibitor of human leukocyte elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) , a 12 kDa serine antiprotease , normally protects the upper airway epithelial surface from attack by neutrophil elastase ( NE ) . ^^^ In the context that a variety of inflammatory lung diseases are characterized by large neutrophil burdens with resultant high levels of NE in the lung , recombinant SLPI ( rSLPI ) , a molecule identical to natural SLPI , may be an effective means to augment the anti NE protective screen of the lung . ^^^ To determine whether intravenous rSLPI will augment respiratory tract and epithelial surface levels of SLPI and anti NE capacity , rSLPI was administered intravenously to sheep and SLPI levels were quantified in plasma , lung lymph ( as a measure of lung interstitial levels ) , lung epithelial lining fluid ( ELF ) , and urine . rSLPI ( 1 g ) was administered over 10 min , and after 30 min plasma levels of SLPI were 8 microM and decreased with a half life of 1 . 8 h . ^^^ Western analysis demonstrated intact SLPI in lymph and ELF , and functional analysis showed increases in lymph and ELF anti NE capacity that paralleled the levels of SLPI . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) , a 12 kD nonglycosylated serine antiprotease with a high capacity for inhibiting neutrophil elastase ( NE ) , is produced by cells of mucosal surfaces including the human lung . ^^^ The molar concentrations of SLPI in total respiratory tract epithelial lining fluid ( ELF ) were 56 + / 10 % that of alpha 1 antitrypsin , suggesting SLPI may be more important for the anti NE protection of the pulmonary epithelial surface than previously thought . ^^^ Consistent with this , evaluation of SLPI in lavage fluid of individuals with cystic fibrosis ( a condition with oxidants and NE on the respiratory epithelium ) showed that the SLPI was degraded . ^^^ However , evaluation of SLPI in normal ELF by molecular sieve analysis and Western analysis demonstrated an intact 12 kD molecule , suggesting that the partial inactivation of SLPI in normals in vivo is not because it is complexed to NE or exposed to oxidants + NE . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The secretory leukoprotease inhibitor ( SLPI ) gene codes for a 12 kD protein that within the lung protects the airway epithelium from neutrophil elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| One candidate for augmenting epithelial anti NE protection is the secretory leukoprotease inhibitor ( SLPI ) . ^^^ In vitro evaluation demonstrated that 96 + / 1 % of the recombinant SLPI ( rSLPI ) molecules were capable of inhibiting NE , with an association rate constant of 7 . 1 + / 0 . 1 10 10 ( 6 ) M 1 . s 1 . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Taking into account that SLPI is a strong inhibitor of several proteases , including leukocyte elastase and cathepsin G , the results suggest that SLPI has a local protective function against proteolytic degradation of the male reproductive tract tissues during inflammation . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The inhibitory capacity of the C terminal domain , ( Asn 55 Ala107 ) SLPI , was as strong as that of full length SLPI against human neutrophil elastase ( NE ) , cathepsin G and chymotrypsin . ^^^ The inhibitory activity of ( Asn 55 Ala107 ) SLPI against the proteolysis of the natural substrates elastin and collagen by NE was comparable with that of full SLPI ( elastin , IC 50 = 907 + / 31 nM for SLPI , 767 + / 33 nM for ( Asn 55 Ala107 ) SLPI ; collagen , IC 50 = 862 + / 36 nM for SLPI , 727 + / 47 nM for ( Asn 55 Ala107 ) SLPI ) . 4 . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND The neutrophil elastase inhibitor , secretory leucocyte protease inhibitor ( SLPI ) , is a potential therapeutic tool in inflammatory lung diseases such as cystic fibrosis and pulmonary emphysema . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| One hundred percent inhibition of NE by pure AAT and SLPI standards occurred at molar ratios of 0 . 91 + / 0 . 03 for AAT to NE and 0 . 83 + / 0 . 02 for SLPI to NE when assayed in buffer with < or = 0 . 15 mol NaCl / L , compared to ratios of 0 . 99 + / 0 . 02 and 1 . 06 + / 0 . 02 , respectively , for assays in buffers with 0 . 50 1 . 00 mol NaCl / L ( p < 0 . 05 for AAT to NE ; p < 0 . 02 for SLPI to NE ) . ^^^ Assays in buffer with < or = 0 . 15 mol NaCl / L indicated that 86 . 9 + / 4 . 1 % of AAT and 100 . 9 + / 4 . 9 % of SLPI in BALF were active against NE , while assays in buffer with 0 . 50 1 . 00 mol NaCl / L showed that 84 . 4 + / 3 . 5 % of AAT and 81 . 6 + / 5 . 9 % of SLPI present were active . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : The purpose was to determine the concentration of leukocyte elastase and SLPI in the cervical mucus plug , cervical tissue , fetal membranes , amniotic fluid and serum from pregnant women . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) , a 12 kDa serine antiprotease , serves as the major inhibitor of neutrophil elastase ( NE ) on the epithelial surface of the upper airways . ^^^ As a control for studies to evaluate the aerosol administration of recombinant SLPI ( rSLPI ) to augment the anti NE defenses of the lung , the status of antioxidants in respiratory epithelial lining fluid ( ELF ) was evaluated . ^^^ To evaluate this concept , rSLPI ( 100 mg ) was aerosolized to sheep , and SLPI , glutathione , anti NE capacity , and anti H2O2 capacity were evaluated in respiratory ELF over a 30 h period . ^^^ As expected , aerosolization of rSLPI increased ELF SLPI levels and anti NE capacity . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In order to elucidate the function of the N terminal domain , we studied the kinetics of reactions of human leukocyte elastase with two recombinant forms of SLPI : the full length inhibitor and the C terminal domain alone . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) , a 12 kD nonglycosylated serine antiprotease , helps to protect the epithelial surface of the airways from the destructive capacity of neutrophil elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Restricted fragments of SLPI were detected using SDS PAGE after treatment with human neutrophil elastase ( NE ) or normal saliva and sequenced at their cleavage sites . ^^^ They were both as active at inhibiting NE as the parent SLPI . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Noncovalent complexes between HLE and either the tight binding secretory leucoprotease inhibitor ( SLPI ) or a slow tight binding peptide difluoroketone inhibitor were not observed by ESI MS . ^^^ SLPI displayed a mass of 11 , 710 Da in the absence and presence of HLE . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The capacity of recombinant human secretory leukocyte proteinase inhibitor ( SLPI ) to inhibit human leukocyte elastase ( HLE ) and cathepsin G ( Cat G ) was investigated and compared with a recombinant truncated form ( carboxyl terminal domain , Asn 55 Ala107 ) called 1 / 2 SLPI . 2 . ^^^ The effects of SLPI and 1 / 2 SLPI were then tested against polymorphonuclear neutrophil ( PMN ) mediated platelet activation , a cell to cell interaction mediated by HLE and Cat G released from PMN . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Results showed that in the BALF and BLF of normal subjects , the SLPI / alpha 1 protease inhibitor ( alpha 1 PI ) ratios ( M / M ) are about 0 . 6 and 6 , respectively and the neutrophil elastase inhibitory capacity of BLF is mainly due to SLPI . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| After rSLPI aerosolization to normal individuals ( 100 mg single dose or 100 mg twice daily for 1 wk ) there was a marked increase in SLPI levels and anti NE capacity in airway epithelial lining fluid ( ELF ) at 1 h , diminishing gradually over 4 to 12 h . ^^^ Interestingly , the ELF SLPI levels and anti NE capacity achieved 12 h after 1 wk of rSLPI aerosols were no different than those 12 h after a single dose of rSLPI , suggesting that rSLPI does not accumulate on the respiratory epithelial surface after aerosolization . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The purposes of this study were to investigate the effect of our recently developed truncated secretory leukoprotease inhibitor ( SLPI ) on bleomycin ( BLM ) induced lung injury and fibrosis in hamsters , which is widely used as a model of interstitial pulmonary fibrosis , and to assess the possible involvement of neutrophil elastase in the pathogenesis of pulmonary fibrosis . ^^^ These results suggest that neutrophil elastase may be implicated in the pathogenesis of BLM induced pulmonary fibrosis and that the truncated SLPI might be a promising therapeutic in the treatment of interstitial pulmonary fibrosis . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| To investigate this notion , we treated awake sheep with a potent neutrophil elastase inhibitor , recombinant secretory leukoprotease inhibitor ( SLPI ) ( 100 mg / day by aerosol ) , during 12 days of CAE ( CAE+SLPI ; n = 7 ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte proteinase inhibitor ( SLPI ) and alpha 1 proteinase inhibitor ( alpha 1 ( PI ) ) can not fully explain the total neutrophil elastase ( NE ) inhibitory capacity detected in bronchoalveolar lavage ( BAL ) fluid , suggesting the existence of other NE inhibitor ( s ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) and alpha 1 protease inhibitor ( alpha 1 PI ) are powerful antiproteases currently under investigation for their potential to protect the lung from neutrophil elastase ( NE ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Three types of endogenous protease inhibitors control the activity of neutrophil elastase , including alpha 1 protease inhibitor ( alpha 1PI ) , alpha 2 macroglobulin and secreted leukoproteinase inhibitor ( SLPI ) [ 8 10 ] . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Because the antiprotease defense in cystic fibrosis ( CF ) airways is overwhelmed by neutrophil elastase ( NE ) , substitution of antiproteases such as secretory leukoprotease inhibitor ( SLPI ) seems to be a reasonable therapeutic approach . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Physiologically , secretory leukoprotease inhibitor ( SLPI ) is the major antiprotease of the epithelium of the upper respiratory tract providing protection against neutrophil elastase ( NE ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) is a low molecular weight serine proteinase inhibitor , notably of neutrophil elastase ( NE ) , which is synthesised and secreted by the pulmonary epithelium . ^^^ SLPI plays an important role in limiting NE induced pulmonary inflammation and , significantly , it also possesses anti HIV activity . ^^^ SLPI is a significant component of the anti NE shield in the lung which has different reactivity from , and is therefore complementary to , the anti NE action of alpha 1 proteinase inhibitor ( alpha 1 PI ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) is one of the major physiological inhibitors protecting respiratory epithelium from attack by excess human leukocyte elastase ( HLE ) , a serine protease released by neutrophils upon activation in response to inflammatory stimuli . ^^^ Kinetic analysis revealed that glycosaminoglycans greatly accelerated the association of oxidized SLPI and HLE , whereas iduronate containing glycosaminoglycans also stabilized the enzyme inhibitor complex formed . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukocyte proteinase inhibitor ( SLPI ) is the main neutrophil elastase ( HLE ) inhibitor found in the upper airways during pulmonary inflammation . ^^^ In addition , we demonstrate that SLPI is the major inhibitor of HLE present in neutrophil cytosol because pre incubation with specific anti SLPI IgG was able to inhibit completely the anti HLE activity of the cytosol . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| A well characterized function of SLPI is inhibition of neutrophil elastase and cathepsin G . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Attention is focused on the balance between destructive enzymes such as neutrophil elastase and protective proteins such as A1AT , secretory leucocyte proteinase inhibitor ( SLPI ) , human elastase inhibitor ( HEI ) and elafin . ^^^ When neutrophil elastase is already attached to the elastin fibres the smaller molecules SLPI and elafin appear to be better inhibitors of this enzyme than larger inhibitors such as A1AT and HEI . ^^^ Furthermore , SLPI and elafin may provide the first line of defence against proteinase attack from neutrophil elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Furthermore , an undersulfated decasaccharide was able to bind SLPI tightly ( Kd approximately 13 nM ) , resulting in its activation and the inhibition of neutrophil elastase and pancreatic chymotrypsin . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Murine SLPI inhibits murine neutrophil elastase and cathepsin G with Ki values of 5 and 0 . 12 nM , respectively , while human SLPI inhibits the both murine serine proteases with Ki ' s of 0 . 02 nM . ^^^ In contrast , murine SLPI inhibits human neutrophil elastase and cathepsin G with Ki values of 1 . 4 and 90 nM , respectively , while human SLPI inhibits the proteases with Ki ' s of 0 . 3 and 10 nM , respectively . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Using two inhibitors , ICI ( Imperial Chemical Industries ) 200 , 355 ( which inhibits both HNE and PR 3 ) and secretory leukoproteinase inhibitor ( SLPI ) ( which inhibits only HNE ) , we showed that PR 3 was enzymatically active in sputum , and its activity , as assessed by SLPI resistant serine proteinase activity , correlated highly with its antigenic concentration measured by ELISA . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Antiproteinases such as alpha 1 proteinase inhibitor ( alpha 1 Pi ) , secretory leukocyte proteinase inhibitor ( SLPI ) and elastase specific inhibitor ( elafin ) control excess production of human neutrophil elastase . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| SLPI is an inhibitor of serine proteases such as leukocyte elastase , cathepsin G , and mast cell chymase . ^^^ Since SLPI is a strong inhibitor of leukocyte elastase , we also focused on the function of elastase in allergic rhinitis . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Recently , NE was found to enhance SLPI mRNA levels while decreasing SLPI protein release in airway epithelial cells . ^^^ Furthermore , glucocorticoids were shown to increase both constitutive and NE induced SLPI mRNA levels . ^^^ The aim of the present study was to investigate the effect of defensins on the production of SLPI and the related NE inhibitor elafin / SKALP in primary bronchial epithelial cells ( PBECs ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| In septic shock , the extent to which lactic acidosis ( LA ) is a consequence of splanchnic lactate overproduction ( SLP ) or impaired hepatic lactate extraction ( HLE ) is not clear . ^^^ We examined SLP and HLE in E . coli sepsis in dogs . ^^^ We further determined the effects of vasopressor treatments , which included phenylephrine , dopamine , norepinephrine , and a combination of dobutamine and norepinephrine treatment , on SLP and HLE in respective groups . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The authors assessed the sputum concentration of the neutrophil chemoattractants interleukin 8 ( IL 8 ) and leukotriene ( LT ) B 4 , myeloperoxidase ( MPO ) as a marker of neutrophil influx , neutrophil elastase activity and its natural inhibitors , alpha1AT and secretory leukoprotease inhibitor ( SLPI ) . ^^^ Patients with alpha1AT deficiency had greater inflammation in the larger airways with increased LTB 4 ( p < 0 . 005 ) , MPO ( p < 0 . 001 ) , neutrophil elastase activity ( p < 0 . 01 ) , protein leak ( p < 0 . 001 ) , and were found to have a lower anti proteinase screen with both reduced sputum alpha1AT ( p < 0 . 001 ) and SLPI concentrations ( p < 0 . 05 ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The goal of the study was to evaluate whether TGF beta 1 is capable of interfering with the expression of the secretory leukoprotease inhibitor ( SLPI ) , the dominating defence of the conducting airways against neutrophil elastase ( NE ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Previous studies using airway epithelial cell lines indicated that neutrophil elastase ( NE ) increases SLPI mRNA transcripts while decreasing SLPI protein release . ^^^ The aim of the present study was to investigate the effect of NE on SLPI and elafin synthesis in cultures of human primary bronchial epithelial cells ( PBEC ) . ^^^ METHODS : Subcultures of human PBEC were incubated with NE , followed by preparation of cell free supernatants and cellular lysates and determination of SLPI and elafin protein levels by enzyme linked immunoadsorbent assay . ^^^ The effect of NE on SLPI mRNA transcripts was determined by Northern blot analysis . ^^^ RESULTS : The results showed that NE increased SLPI mRNA expression while decreasing SLPI protein release . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Previously we reported that DNA from sputum promotes the inhibition of human leukocyte elastase ( HLE ) by native secretory leukoprotease inhibitor ( SLPI ) . ^^^ With sputum DNA at 5 microg / ml , a concentration much lower than those in vivo , the inhibition constant ( K ( 1 ) ) of oxidized SLPI against HLE is reduced from 31 nM to 23 to 920 pM , as compared with the K ( 1 ) of native SLPI , 58 pM , under the same conditions . ^^^ DNA binding sites on HLE also participate in the upregulation of oxidized SLPI activity . ^^^ Data from this and our previous studies demonstrate that sputum DNA facilitates the association of HLE with native and oxidized SLPI , whereas it delays the association of HLE with alpha ( 1 ) PI . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Although human neutrophil elastase ( HNE ) at low concentrations increased SLPI secretion above the control level ( i . e . , 149 % of control level at 10 ( 11 ) M ) , HNE at high concentrations significantly decreased it below the control level ( i . e . , 16 % of control level at 10 ( 7 ) M ) . ^^^ The decrease in SLPI concentration was shown to result from the degradation of SLPI by excessive HNE . ^^^ Both HNE and methacholine significantly increased the level of SLPI mRNA in submucosal glands in a dose dependent manner ( i . e . , 357 % of control level at 10 ( 7 ) M and 175 % of control level at 10 ( 5 ) M , respectively ) . ^^^ These findings indicate that human airway submucosal glands can transcribe 30 fold or more SLPI mRNA than the superficial epithelium and that SLPI mRNA transcription and secretion are regulated by both HNE and muscarinic receptors . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| While inhibition of neutrophil elastase is a function common to both alpha ( 1 ) PI and SLPI , we showed previously that they exhibit different patterns of protection against antigen induced changes in airway function in allergic sheep . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The naturally occurring neutrophil elastase inhibitors , alpha 1 proteinase inhibitor ( alpha1PI ) , secretory leukocyte proteinase inhibitor ( SLPI ) , and elafin , are potential therapeutic agents in the treatment of neutrophil mediated lung disease . ^^^ The effect of MMP 8 on SLPI and elafin was assessed by determining the neutrophil elastase inhibitory capacity ( NEIC ) and electrophoretic protein profile of both inhibitors following exposure to purified MMP 8 . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Interleukin ( IL ) 1beta , IL 6 , IL 8 , leukotriene B ( 4 ) ( LTB ( 4 ) ) , secretory leukoprotease inhibitor ( SLPI ) , alpha 1 antitrypsin ( alpha ( 1 ) AT ) , and tumour necrosis factor alpha ( TNFalpha ) were measured by ELISA , and neutrophil elastase ( NE ) and myeloperoxidase ( MPO ) by chromogenic substrate assay . ^^^ RESULTS : Median levels of IL 1beta , IL 6 , IL 8 , SLPI , and NE were similar in the DTT and NaCl treated samples . ^^^ DTT had no effect on the assay standards for IL 1beta , IL 8 or TNFalpha , but at higher concentrations it did affect IL 6 , SLPI , NE , and LTB ( 4 ) standards ( 43 % , 70 % , 76 % and 643 % of control value for top standard , respectively ) and at all concentrations DTT completely abolished MPO activity . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Concentrations of two neutrophil elastase inhibitors , alpha 1 proteinase inhibitor ( PI ) and secretory leukoproteinase inhibitor ( SLPI ) , as well as neutrophil elastase ( NE ) activity and NE inhibitory capacity ( NEIC ) were quantified in all lavage fluids . ^^^ Neutrophil elastase inhibitory capacity activity followed the same gradient and correlated positively and consistently with SLPI , suggesting that this inhibitor makes an important contribution to the regulation of elastolytic activity in the healthy human respiratory tract . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Secretory leukoprotease inhibitor ( SLPI ) protects tissue against the destructive action of neutrophil elastase at the site of inflammation . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Levels and activities of alpha 1 antitrypsin ( A1AT ) , secretory leucoprotease inhibitor ( SLPI ) , and neutrophil elastase ( NE ) were quantified . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| We examined its ability to inhibit NF kappaB activation induced by lipoteichoic acid and investigated the effects of oxidation or complex formation with neutrophil elastase on SLPI ' s anti inflammatory properties in U 937 myelomonocytic cells and macrophages . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Platelet SLPI inhibits neutrophil elastase , an activity that is reduced when beta 1 tubulin is absent . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Release of neutrophil elastase and plasma levels of the natural NE antagonists alpha ( 1 ) proteinase inhibitor ( API ) and secretory leukocyte proteinase inhibitor ( SLPI ) were measured . ^^^ NE and SLPI concentrations were measured by ELISA , API and fibronectin plasma levels were determined by nephelometry . ^^^ The imbalance between NE and the NE inhibitors API and SLPI may increase the risk for tissue damage due to granulocyte activation after cardiac surgery . . ^^^ RESULTS : Neutrophil elastase plasma concentrations rose during ECC ( 245 + / 107 microg / ml versus 44 + / 14 microg / ml after induction , p < 0 . 001 ) , whereas SLPI and API were decreased after onset of ECC . 24 h after ECC SLPI ( 54 + / 17 ng / ml versus 41 + / 10 ng / ml , p < 0 . 05 ) and API ( 3 + / 0 . 5 g / l versus 1 . 6 + / 0 . 3 g / l , p < 0 . 05 ) increased significantly compared to baseline values . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| This has enabled us to demonstrate that ovine SLPI is correctly processed ( Western blot analysis and SELDI TOF mass spectrometry analysis ) and has biological antihuman neutrophil elastase activity . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| This review will address more particularly the pleiotropic functions of low molecular mass neutrophil elastase inhibitors [ SLPI ( secretory leucocyte proteinase inhibitor ) and elafin ] and , more specifically , their role in the development of the adaptive immune response . . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| Rgps proteolysis of SLPI disrupted SLPI functions , which normally suppresses neutrophil elastase and neutralizes pro inflammatory effects of bacterial cell wall compounds in cultured human gingival fibroblasts . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| The lower SLPI levels in antral biopsies of H . pylori positive subjects were associated with a 30 fold increase ( p < 0 . 01 ) in neutrophil elastase activity , and a significant negative correlation was demonstrated for both parameters ( R= 0 . 63 , p=0 . 0002 ) . ^^^ |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P03973 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
|