| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| Like EGF URO , TGF alpha was a potent stimulator of femoral arterial blood flow in vivo ; and , when added to the tissue bath in vitro before an agonist , TGF alpha like EGF URO was a potent inhibitor of the contractile responses of helical coronary arterial strips to various smooth muscle agonists : norepinephrine ( NE ) , prostaglandin F 2 alpha ( PGF alpha ) and potassium chloride ( KCl ) . ^^^ The inhibitory action of EGF URO for agonist ( NE , PGF 2 alpha or KCl ) mediated contraction was diminished with repeated exposure of the tissue to EGF URO , whereas the inhibitory action of TGF alpha persisted with repetitive tissue exposure . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| The female TGF alpha mice showed elevated levels of norepinephrine ( NE ) in the hypothalamus and serotonin ( 5 HT ) in the cortex and brain stem when compared with nontransgenic CD 1 females . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| Amongst these , endopeptidase activity produces a similar product profile from the nonapeptide substrate to that of human leukocyte elastase , an enzyme previously shown to be capable of releasing a bioactive , mature form of TGF alpha from its cell bound precursor . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| HNE induced MUC5AC production was inhibited by a neutralizing transforming growth factor alpha ( TGF alpha , an EGFR ligand ) antibody and by a neutralizing EGFR antibody but not by oxygen free radical scavengers , further implicating TGF alpha and ligand dependent EGFR activation in the response . ^^^ HNE decreased pro TGF alpha in NCI H 292 cells and increased TGF alpha in cell culture supernatant . ^^^ From these results , we conclude that HNE induced MUC5AC mucin production occurs via its proteolytic activation of an EGFR signaling cascade involving TGF alpha . . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| Activated neutrophils recruited to the airways ( and their secreted products ) play several key roles in EGFR dependent mucus hypersecretion : ( 1 ) activated neutrophils secrete tumor necrosis factor ( TNF ) alpha , which induces EGFR expression in airway epithelial cells ; ( 2 ) activated neutrophils release reactive oxygen species , which activate EGFR ; ( 3 ) neutrophil elastase cleaves the EGFR proligand , pro transforming growth factor ( TGF ) alpha , releasing mature TGF alpha which activates EGFR in a ligand dependent fashion ; and ( 4 ) neutrophil elastase causes potent goblet cell degranulation . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We demonstrate that in BLM treated mice ( 1 ) the development of elastolytic emphysema precedes that of fibrosis ; ( 2 ) significant amount of elastase in alveolar interstitium is associated with an increased expression of TGF beta and TGF alpha ; and finally , ( 3 ) emphysematous and fibrotic lesions can be significantly attenuated by using a protease inhibitor active against neutrophil elastase . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| Previous studies showed that HNE induces MUC5AC mucin production involving reactive oxygen species ( ROS ) generation and TGF alpha dependent epidermal growth factor receptor ( EGFR ) activation in human airway epithelial cells . ^^^ We hypothesize that HNE activates TACE via ROS generation , resulting in cleavage of pro TGF alpha , EGFR activation , and MUC5AC mucin expression in airway epithelial cells . ^^^ Here we show that in human airway epithelial cells HNE increases TGF alpha release , EGFR phosphorylation , and MUC5AC mucin expression , effects that were attenuated by TACE inhibitor TAPI 1 and by specific knockdown of TACE expression with small interfering RNA , implicating TACE in HNE induced responses . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| Half life of tropoelastin mRNA in RFL 6 cells is > 24 h , but it is decreased to approximately 8 h by addition of TGF beta neutralizing antibody , EGF , TGF alpha , or NE . ^^^ Tropoelastin mRNA destabilization by NE , EGF , or TGF alpha is abolished by AG 1478 or U 0126 . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| It was further demonstrated that NE induced EGFR phosphorylation was transactivated through TGF alpha , using ELISA . ^^^ NE increased the cleavage of TGF alpha from the cell surface 30 fold compared with the cells without treatment . ^^^ Interestingly , sivelestat significantly reduced NE induced EGFR phosphorylation and ERK1 / 2 activation and completely blocked the release of TGF alpha from the TMK 1 cell surface . ^^^ These results indicate that sivelestat suppresses the growth of gastric cancer cells by inhibiting the release of TGF alpha stimulated by NE , which often occurs after surgical stresses . . ^^^ Sivelestat , a specific neutrophil elastase inhibitor , suppresses the growth of gastric carcinoma cells by preventing the release of transforming growth factor alpha . ^^^ |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P01135 and P08246 |
Pubmed |
SVM Score :0.0 |
| NA |
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