Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.62796336
Interactions of neutrophil elastase , serum trypsin inhibitory activity , and smoking history as risk factors for chronic obstructive pulmonary disease in patients with MM , MZ , and ZZ phenotypes for alpha 1 antitrypsin . 0.62796336^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.60583338
Incubating leukocyte elastase with plasma elastase inhibitor alpha 1 antitrypsin prevented the loss of recombinant TNF bioactivity and blocked the degradation of this cytokine . 0.60583338^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.67767804
METHOD : Alpha 1 antitrypsin from a female patient aged 75 years with the rare genotype PLowell NullBellingham was studied for its ability to inhibit human neutrophil elastase in a time dependent manner . 0.67767804^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.54511195
AAT formed a complex with neutrophil elastase . 0.54511195^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.65482793
Western blot analysis showed that this murine muscle secreted human AAT ( hAAT ) formed a complex with human neutrophil elastase in a dose dependent manner . 0.65482793^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
METHODS : Neutrophil elastase activity , sputum concentrations of the serum derived inhibitors alpha 1 antiproteinase and alpha 2 macroglobulin , and the sputum to serum ratios of albumin and C reactive protein concentration were measured in 26 patients with bronchiectasis . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In addition to its direct elastolytic properties , this metalloelastase may also promote elastolysis by cleaving alpha 1 antiproteinase and thus protecting neutrophil elastase from inhibition . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Plasma concentrations of neutrophil elastase alpha 1 antiproteinase , tumour necrosis factor alpha ( TNF alpha ) and serum C reactive protein ( CRP ) were increased in the 7 days prior to death ( P < 0 . 05 ) when compared with a period of clinical stability during the preceding 6 months . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Hemoglobin , neutrophil elastase , and alpha 1 antiproteinase ( alpha 1 PI ) oxidized to eliminate its antiproteinase activity all stabilized large particles against conversion . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Multiple forms of alpha 1 antiproteinase in the rabbit plasma implicate the unknown functions other than the inhibition of neutrophil elastase . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Spirometry and circulating concentrations of C reactive protein , neutrophil elastase alpha 1 antiproteinase complex , catecholamines , non esterified fatty acids and glycerol were determined . ^^^ Following treatment , 1 s forced expiratory volume ( FEV 1 ) and weight increased , while C reactive protein ( P < 0 . 0001 ) and neutrophil elastase alpha 1 antiproteinase complex concentrations ( P < 0 . 0001 ) were reduced . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The stoichiometry of inhibition of human pancreatic elastase 2 by human alpha 1 antitrypsin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Total protein , alpha 1 antitrypsin , alpha 2 macroglobulin , amylase , methemalbumin , tryptic amidase activity , radioimmunoassayable elastase 2 , and three lysosomal hydrolases were determined in the ascites fluid from patients with acute pancreatitis . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The inactivation of elastase 2 employed as radioiodinated tracer with an active site specific reagent ( phenylmethanesulfonyl fluoride ) was necessary to prevent its binding by serum alpha 1 antitrypsin and alpha 2 macroglobulin while maintaining its immunoreactivity . ^^^ While a minor amount of elastase 2 is eluted in a position consistent with alpha 1 antitrypsin elastase 2 complex , no free elastase or free proelastase is detectable . ^^^ Addition of exogenous elastase 2 to normal serum prior to gel filtration on G 200 produced an increase only in the peak of radioimmunoassayable elastase bound to alpha 1 antitrypsin . ^^^ In vitro experiments have demonstrated that while elastase 2 bound to alpha 1 antitrypsin is immunologically reactive , alpha 2 macroglobulin bound elastase 2 cross reacts less than 2 % in this radioimmunoassay . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neither trypsin nor neutrophil elastase were present in the active fraction whereas , among the possible plasma proteinase inhibitors tested , immunoreactivity was observed only in the presence of alpha 1 antitrypsin ( alpha 1 AT ) antiserum . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The relative concentrations and absolute amounts of neutrophil elastase and its two inhibitors , alpha 2 macroglobulin ( alpha 2 M ) and alpha 1 antitrypsin ( alpha 1 AT ) , were determined in gingival crevicular fluid ( GCF ) collected from six dental students who refrained from brushing the upper left or right quadrant during three weeks . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutrophil elastase ( NE ) and its complex with alpha 1 antitrypsin were quantified in ear discharges from 15 patients with chronic suppurative otitis media ( CSOM ) , and their levels were compared to those in middle ear effusions from 10 pediatric patients with chronic otitis media with effusion ( OME ) . ^^^ The mean value of NE alpha 1 antitrypsin complex was 13 . 7 + / 8 . 8 micrograms / mL in CSOM and 7 . 8 + / 4 . 0 micrograms / mL in OME . ^^^ Neutrophil elastase and its complex with alpha 1 antitrypsin in the pathogenesis of chronic suppurative otitis media . ^^^ Neutrophil elastase ( NE ) and its complex with alpha 1 antitrypsin were quantified in ear discharges from 15 patients with chronic suppurative otitis media ( CSOM ) , and their levels were compared to those in middle ear effusions from 10 pediatric patients with chronic otitis media with effusion ( OME ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
We describe a method to measure human leukocyte elastase ( HLE ) inhibitory capacity and compared it with porcine pancreatic elastase ( PPE ) inhibitory capacity and with a turbidimetric method using a specific antibody to alpha 1 antitrypsin ( AAT ) , all performed on a Cobas Bio centrifugal analyser . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Different transcriptional initiation sites used in three different cell types . alpha 1 Antitrypsin ( alpha 1 AT ) is plasma glycoprotein that constitutes the principle inhibitor of neutrophil elastase in tissue fluids . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Formation of the covalently stabilized alpha 1 antitrypsin ( alpha 1 AT ) neutrophil elastase complex , the archetype of serpin enzyme complexes , results in a structurally rearranged alpha 1 AT molecule that possesses chemo attractant activities , mediates an increase in synthesis of alpha 1 AT by mononuclear phagocytes and hepatocytes , and is more rapidly cleared from the circulation than is the native alpha 1 AT molecule . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The emphysema of alpha 1 antitrypsin ( alpha 1AT ) deficiency is conceptualized to result from insufficient alpha 1AT allowing neutrophil elastase to destroy lung parenchyma . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The molar concentrations of SLPI in total respiratory tract epithelial lining fluid ( ELF ) were 56 + / 10 % that of alpha 1 antitrypsin , suggesting SLPI may be more important for the anti NE protection of the pulmonary epithelial surface than previously thought . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In cystic fibrosis neutrophil dominated inflammation on the respiratory epithelial surface results in a chronic epithelial burden of the destructive enzyme , neutrophil elastase . alpha 1 antitrypsin ( alpha 1AT ) , the main inhibitor of neutrophil elastase in lung , was given in aerosol form to 12 cystic fibrosis patients . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The net balance of neutrophil elastase and its inhibitor , alpha 1 antitrypsin ( alpha 1 AT ) , is a critical determinant of connective tissue turnover during homeostasis and in disease states . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
During the formation of an inhibitory complex with neutrophil elastase , alpha 1 antitrypsin ( alpha 1 AT ) undergoes a structural rearrangement and the resulting alpha 1 AT elastase complex becomes endowed with chemoattractant activities , mediates an increase in synthesis of alpha 1 AT , and is rapidly cleared from the circulation . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Immunoreactive neutrophil elastase ( NE ) and its complex with alpha 1 antitrypsin ( AT ) was measured by double antibody enzyme linked immunosorbent assay ( ELISA ) in nasal secretions of chronic sinusitis ( CS ) . ^^^ Neutrophil elastase and its complex with alpha 1 antitrypsin in soluble and insoluble fractions of nasal secretions of chronic sinusitis . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Two different horseradish peroxidase ( HRP ) labelled antibodies used as the second antibody were anti NE HRP for measuring total ( free + complexed ) NE level and anti alpha 1 antitrypsin ( AT ) HRP for complexed NE level . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Double labeling with colloidal gold showed that alpha 1 antitrypsin is localized at the same site as neutrophil elastase , i . e . , within primary granules . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Diagnosis of the hereditary disorder alpha 1 antitrypsin ( alpha 1AT ) deficiency is critically dependent on quantification of serum levels of alpha 1AT , a 52 kDa antiprotease that serves to protect the lung from destruction by neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Gap formation was prevented by alpha 1 antitrypsin , N methoxysuccinyl Ala Ala Pro Val chloromethylketone , or 10 % serum , was mimicked by PMN elastase ( 24 micrograms / ml ) , but not by hydrogen peroxide in concentrations up to 10 mM , or superoxide generated by xanthine / xanthine oxidase , and was reversible within 24 h of removal of elastase and exposure to fresh medium . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The serum protein alpha 1 antitrypsin ( alpha 1 AT ) serves as the major inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The effects of HNE ( 500 micrograms / ml ) were completely abolished by adding alpha 1 antitrypsin ( 5 mg / ml ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Proteolytic inactivation of human alpha 1 antitrypsin by human stromelysin . alpha 1 Antitrypsin ( alpha 1AT ) is the main physiological inhibitor of neutrophil elastase , a serine protease which has been implicated in tissue degradation at inflammatory sites . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Cigarette smoking is associated with a reduction in the association rate constant of lung alpha 1 antitrypsin for neutrophil elastase . ^^^ To evaluate this hypothesis we compared the time dependent kinetics of the inhibition of neutrophil elastase by lung alpha 1AT from healthy , young cigarette smokers ( n = 8 ) and nonsmokers ( n = 12 ) . alpha 1 antitrypsin was purified from lavage fluid using affinity and molecular sieve chromatography , and the association rate constant ( k assoc ) for neutrophil elastase quantified . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Characterization of the molecular basis of the alpha 1 antitrypsin F allele . alpha 1 Antitrypsin ( alpha 1AT ) , the major serum inhibitor of neutrophil elastase , is a highly polymorphic serum protein associated with characteristic isoelectric focusing ( IEF ) patterns for most variants . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Protection of normal tissues from the destructive capacity of NE is provided by alpha 1 antitrypsin ( alpha 1 AT ) , a 52 Kd serine antiprotease produced by hepatocytes and mononuclear phagocytes . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The importance of the destructive element in emphysema and the gradual focusing on neutrophil elastase as a key enzyme in the pathogenesis of emphysema in alpha 1 antitrypsin deficiency is emphasized . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin ( AAT ) deficiency is a genetic disease in which low serum and lung levels of the antiprotease AAT cause a deficiency of the anti elastase defensive screen of the lower respiratory tract such that neutrophil elastase is free to degrade the connective tissue of the lung , eventually resulting in emphysema . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Formation of the covalently stabilized complex of alpha 1 antitrypsin ( alpha 1 AT ) with neutrophil elastase , the archetype of serine proteinase inhibitor serpin enzyme complexes , is associated with structural rearrangement of the alpha 1 AT molecule and hydrolysis of a reactive site peptide bond . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 Antitrypsin ( alpha 1 AT ) is similar to other members of the serine protease inhibitor ( serpin ) supergene family in that it undergoes structural rearrangement during the formation of a covalently stabilized inhibitory complex with its cognate enzyme , neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Coal workers had significantly elevated levels of neutrophil elastase in BAL fluid complexed with alpha 1 antitrypsin ( P less than 0 . 01 ) and normal levels of alpha 1 antitrypsin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Characterization of the normal alpha 1 antitrypsin allele Vmunich : a variant associated with a unique protein isoelectric focusing pattern . alpha 1 Antitrypsin ( alpha 1AT ) , the major serum inhibitor of neutrophil elastase , is a highly polymorphic protein associated with isoelectric focusing ( IEF ) patterns typical for each variant . alpha 1AT Vmunich , a previously unreported normal alpha 1AT variant , has a unique IEF banding pattern in which the 7 and 8 alpha 1AT protein bands focus with the normal M type 7 and 8 bands , despite the fact that the major fraction of the Vmunich protein focuses in the `` 5 ' ' region of the IEF gel . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The blood concentrations of granulocytic elastase ( PMN E ) , alpha 1 antitrypsin and alpha 2 macroglobulin were examined to evaluate their clinical significance in patients who underwent hepatectomy due to liver cancer with liver cirrhosis ( n = 31 , Group A ) , due to liver cancer with chronic hepatitis ( n = 5 , Group B ) and without hepatic complications ( n = 6 , Group C ) and other major surgeries ( n = 10 , Group D ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In order to study the pathogenic mechanisms of adult respiratory distress syndrome ( ARDS ) , the neutrophil aggregation activity , platelet aggregation rate and levels of thromboxane , prostacyclin , superoxide dismutase , lipoperoxides , neutrophil elastase , alpha 1 antitrypsin and angiotensin conversion enzyme were measured in 84 experimental dogs . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Smokers did not differ significantly from nonsmokers in the antigenic alpha 1 antitrypsin ( alpha 1AT ) concentrations ( 0 . 67 + / 0 . 04 versus 0 . 73 + / 0 . 09 nmol alpha 1AT / mg protein ) , in the neutrophil elastase inhibitory capacity ( NEIC ) ( 0 . 59 + / 0 . 03 versus 0 . 52 + / 0 . 05 nmol NEIC / mg protein ) , or in the porcine pancreatic elastase inhibitory capacity ( PPEIC ) ( 0 . 36 + / 0 . 03 versus 0 . 42 + / 0 . 05 nmol PPEIC / mg protein ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin ( alpha 1AT ) , a highly pleomorphic 52 kD glycoprotein , functions chiefly as the major inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The alpha 1 antitrypsin gene and emphysema . alpha 1 Antitrypsin ( alpha 1 AT ) is the major endogenous inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The alpha 1 antitrypsin gene and its deficiency states . alpha 1 antitrypsin , a 52 kDa antiprotease , provides the major defense to the lower respiratory tract against the ravages of neutrophil elastase , a powerful serine protease . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The results were an activity increase of leukocyte elastase and trypsin inhibition in serum as well as doubling of alpha 1 antitrypsin in sputum . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 Antitrypsin ( alpha 1AT ) deficiency is characterized by insufficient amounts of alpha 1AT to protect the lower respiratory tract from neutrophil elastase , resulting in emphysema . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Serum elastase , neutrophil elastase and alpha 1 antitrypsin ( A 1 AT ) were determined in 20 patients undergoing elective coronary artery bypass grafting . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Oxidants spontaneously released by alveolar macrophages of cigarette smokers can inactivate the active site of alpha 1 antitrypsin , rendering it ineffective as an inhibitor of neutrophil elastase . ^^^ The possible role of alveolar macrophages in contributing to this imbalance was investigated by evaluating the ability of cigarette smokers ' alveolar macrophages to inactivate alpha 1 antitrypsin ( alpha 1AT ) , the major anti neutrophil elastase of the human lower respiratory tract . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Characterization of the M 1 ( Ala 213 ) type of alpha 1 antitrypsin , a newly recognized , common `` normal ' ' alpha 1 antitrypsin haplotype . alpha 1 Antitrypsin ( alpha 1AT ) is a highly pleomorphic 52 kDa serum glycoprotein that functions as the major inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutrophil elastase activity was not detected in any ARDS lavages , although elastase was antigenically present in most samples and appeared to be complexed to alpha 1 antitrypsin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 Antitrypsin ( alpha 1AT ) deficiency is a hereditary disorder characterized by reduced serum levels of alpha 1AT , resulting in destruction of the lower respiratory tract by neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Immunoreactive polymorphonuclear leukocyte elastase in complex with alpha 1 antitrypsin in Kawasaki disease . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Homozygous inheritance of the null bellingham alpha 1 antitrypsin ( alpha 1AT ) gene is associated with early onset emphysema , resulting from the lack of alpha 1AT to protect the lung from neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Evaluation of the S type of alpha 1 antitrypsin as an in vivo and in vitro inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The major physiological role of the serine protease inhibitor alpha 1 antitrypsin ( alpha 1 AT ) is to protect elastic fibers in the lung from excessive hydrolysis by neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The hereditary disorder alpha 1 antitrypsin ( alpha 1AT ) deficiency results in the development of emphysema due to a diminished anti neutrophil elastase screen of the lower respiratory tract . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The `` deficiency ' ' group of alpha 1 antitrypsin ( alpha 1AT ) alleles is characterized by alpha 1AT genes that code for alpha 1AT present in serum but in amounts insufficient to protect the lower respiratory tract from progressive destruction by its burden of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin ( alpha 1AT ) , a 52 , 000 mol wt serum glycoprotein produced by hepatocytes and mononuclear phagocytes , functions as the major inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Z type alpha 1 antitrypsin is less competent than M 1 type alpha 1 antitrypsin as an inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Current concepts of the pathogenesis of emphysema suggest that an imbalance between neutrophil elastase and alpha 1 antitrypsin in the lung allows neutrophil elastase to work unimpeded to destroy the alveolar structures . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Although alpha 1 proteinase inhibitor ( alpha 1 antitrypsin ) is widely thought to protect lung elastin against the elastolytic action of leukocyte elastase , there is only circumstantial evidence for such a protective role . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The primary function of alpha 1 antitrypsin ( alpha 1 AT ) , an antiprotease produced by the liver , is the inhibition of neutrophil elastase , a protease capable of hydrolysing most connective tissue components . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Evaluation of recombinant DNA directed E . coli produced alpha 1 antitrypsin as an anti neutrophil elastase for potential use as replacement therapy of alpha 1 antitrypsin deficiency . alpha 1 antitrypsin ( alpha 1AT ) deficiency is an inherited disorder almost always associated with the development of panacinar emphysema in the fourth to fifth decades . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The results of the technique are compared with those described with staining for muramidase ( lysozyme ) , alpha 1 antitrypsin , leukocyte elastase , and naphthol AS D chloroacetate esterase , and with certain monoclonal antisera directed against granulocyte determinants . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
These studies demonstrated that : ( a ) alpha 1 antitrypsin is the major antielastase of the normal human lower respiratory tract ; ( b ) alpha 2 macroglobulin , a large serum antielastase , and the bronchial mucous inhibitor , an antielastase of the central airways , do not contribute to the antielastase protection of the human alveolar structures ; ( c ) individuals with PiZ alpha 1 antitrypsin deficiency have little or no alpha 1 antitrypsin in their lower respiratory tract and have no alternative antiprotease protection against neutrophil elastase ; and ( d ) the lack of antiprotease protection of the lower respiratory tract of PiZ individuals is a chronic process , suggesting their vulnerability to neutrophil elastase is always present . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The interaction of alpha 1 antitrypsin with trypsin , chymotrypsin and human leukocyte elastase as revealed by end group analysis . ^^^ Complexes of alpha 1 antitrypsin with trypsin , alpha chymotrypsin , and human leukocyte elastase were purified and examined for amino terminal sequences . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The emphysema associated with the inherited serum deficiency of alpha 1 antitrypsin appears to result from an imbalance between neutrophil elastase and its major inhibitor within the alveolar structures . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The sequence of alpha 1 antitrypsin is in keeping with its role as a tissue scavenger of leukocyte elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Among the oxidants released are potent chlorinated compounds , such as hypochlorous acid and chloramines , which induce tissue cell damage and inactivate protease inhibitors , particularly alpha 1 antitrypsin , the specific inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Compared with PMN elastase or IL 6 , increased plasma concentrations of such acute phase proteins as alpha 1 antitrypsin or CRP , and consumption of the protease inhibitor alpha 2 macroglobulin , are later events that can be detected only 1 to 4 days later . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Arg 15 aprotinin ( 3 . 1 microM ) , Ala 357 , Arg 358 alpha 1 antitrypsin ( 2 . 6 microM ) , and soybean trypsin inhibitor ( 48 . 0 microM ) either completely or partially inhibited the generation of kallikrein C 1 inhibitor complexes but were less effective inhibitors of human neutrophil elastase release . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Samples of pleural effusion were obtained 3 and 24 h postoperatively and samples of the peripheral blood were obtained preoperatively , 3 h , 24 h , and 1 w postoperatively , then the neutrophil counts , the levels of neutrophil elastase and alpha 1 antitrypsin by enzyme linked immunosorbent assay , the activity of neutrophil elastase using succinyl ( L alanine ) 3 p nitroanilide were measured . ^^^ In conclusion , there is an imbalance between the levels of neutrophil elastase and alpha 1 antitrypsin in pleural effusion after lobectomy , the imbalance resulting in the increased activity of neutrophil elastase which probably is relating to tissue injury in the pleural spaces . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
This oxidant is also capable of inactivating the specific inhibitor of neutrophil elastase ( alpha 1 antitrypsin ) , thereby favouring digestion of the connective matrix . 2 . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin Pittsburgh ( Met 358 > Arg ) inhibits the contact pathway of intrinsic coagulation and alters the release of human neutrophil elastase during simulated extracorporeal circulation . ^^^ Most importantly , Arg 358 alpha 1 antitrypsin decreased the release of 1 . 11 + / 0 . 16 micrograms / ml human neutrophil elastase by 43 % . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Intratracheal ( i . t . ) or intravenous ( i . v . ) administration of FR 901451 , a potent inhibitor of human leukocyte elastase ( HLE ) prevented HLE induced lung hemorrhage in hamsters with ED 50 values of 10 . 5 micrograms / site and 8 . 1 mg / kg , respectively . alpha 1 Antitrypsin ( alpha 1 AT ) also showed inhibitory effect in this model . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
METHODS : We examined PMN elastase complexed with alpha 1 antitrypsin ( alpha 1 AT ) , chymotrypsin , and alpha 2 macroglobulin by ELISA in feces and plasma . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
All patients had markedly elevated plasma polymorphonuclear leucocyte elastase ( PMN elastase ) levels with mild depletion of alpha 2 macroglobulin but normal concentrations of alpha 1 antitrypsin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
We measured total neutrophil elastase burden , elastase activity , and alpha 1 antitrypsin ( alpha 1AT ) in bronchoalveolar lavage ( BAL ) fluid and we evaluated the in vitro interleukin 8 ( IL 8 , a potent chemoattractant for neutrophils ) secretion by alveolar macrophages ( AM ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
BACKGROUND : Alpha 1 antitrypsin is the body ' s major inhibitor of human neutrophil elastase , a powerful proteolytic enzyme capable of degrading the common tissue components . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Matrilysin is much more efficient than other matrix metalloproteinases in the proteolytic inactivation of alpha 1 antitrypsin . alpha 1 antitrypsin , the primary physiologic inhibitor of human leukocyte elastase , is proteolytically inactivated by several matrix metalloproteinases including interstitial collagenase , stromelysin and 92 kDa gelatinase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Recent studies have shown that alpha 1 antitrypsin ( alpha 1 AT ) from Z antitrypsin deficiency subjects has a slightly lower association rate constant with neutrophil elastase ( NE ) than alpha 1 AT from normal subjects , although it is unknown whether this is of clinical importance . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
GCF eluates of periodontitis group 1 and controls , prepared by a technique that lysed polymorphonuclear leucocytes ( PMN ) in the samples , were assayed for functional neutrophil elastase ( NE ) and immunoreactive alpha 1 antitrypsin ( alpha 1 AT ) and alpha 1 antitrypsin neutrophil elastase complex ( alpha 1 AT NE ) . ^^^ Inhibition of crevicular fluid neutrophil elastase by alpha 1 antitrypsin in periodontal health and disease . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Expression and structural analysis of a novel highly inducible gene encoding alpha 1 antitrypsin in rabbit . alpha 1 Antitrypsin is a major plasma proteinase inhibitor whose primary function is to control the proteolytic activity of neutrophil elastase that hydrolyzes structural proteins . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Evaluation of serine alpha 1 antitrypsin and polymorphonuclear leukocyte elastase contents and their immunogenetic correlation in psoriasis . ^^^ The purpose of our study was to quantify the serum content of alpha 1 antitrypsin ( alpha 1 AT ) and polymorphonuclear leukocyte elastase ( PMN E ) in of 21 patients affected by active and stationary psoriasis , and 12 normal controls . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
We have examined the relationship between gut mucosal hypoperfusion , endotoxin core antibodies ( EndoCAb ) , neutrophil elastase alpha 1 antitrypsin complexes ( NE ) and components of the contact system during elective major surgery . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutrophil polymorphonuclear leukocytes ( PMN ) can inactivate the PMN elastase inhibitor alpha 1 antitrypsin ( A1AT ) proteolytically , by using metalloproteinases normally stored as zymogens in myeloperoxidase ( MPO ) negative granules . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
A protein structural approach to the solution of biological problems : alpha 1 antitrypsin as a recent example . alpha 1 Antitrypsin is a circulating serine proteinase inhibitor that protects the lungs against proteolysis by the enzyme neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
At this time circulating C reactive protein was increased in all 6 and neutrophil elastase alpha 1 antitrypsin complex ( elastase complex ) was increased in 5 patients compared with healthy controls . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Mutation of Ala 347 to threonine in alpha 1 antitrypsin did not affect the kinetic behavior of the protein with trypsin or human leukocyte elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The protection of a body ' s alpha 1 antitrypsin shield from serpinolytic activity of collagenase and matrix metallproteinases can result in inhibition of serine proteases such as neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin deficiency : an overview . 1 . alpha 1 antitrypsin is an antiprotease that inhibits the neutrophil elastase enzyme , and belongs to a family of structurally related serine proteinase inhibitors ( serpins ) . ^^^ In normal conditions , alpha 1 antitrypsin protects the lungs from destruction by the proteolytic neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Cell specific expression of alpha 1 antitrypsin in human intestinal epithelium . alpha 1 Antitrypsin ( alpha 1 AT ) is an acute phase plasma protein predominantly derived from the liver which inhibits neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin is a glycoprotein which inactivates proteolytic enzymes , especially neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Instead of shifting the specificity of alpha 1 antitrypsin towards the proteases of the contact activation and complement systems , we attempted to obtain a C 1 inhibitor species which resists proteolytic inactivation by HNE . 12 recombinant C 1 inhibitor variants were produced with mainly conservative substitutions at the cleavage sites for HNE , 440 Ile and / or 442 Val . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
An ELISA for neutrophil elastase ( ELA ) in complex with alpha 1 protease inhibitor ( PI ) ( alpha 1 antitrypsin ) was developed in microtitre plates and compared to the ELISA kit from MERCK ( 2 h version ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
IL 8 , total neutrophil elastase ( NE ) , free elastase activity , alpha 1 antitrypsin ( alpha 1 AT ) , and total leukocyte and neutrophil counts were evaluated in bronchoalveolar lavage fluids ( BALF ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The production of chemotactic activity is dose and time dependent and can be blocked by preincubation of NE with alpha 1 antitrypsin ( alpha1AT ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The serum levels of cytokines ( interleukin 1 beta ; IL 1 beta , interleukin 6 ; IL 6 , tumor necrosis factor alpha ; TNF alpha ) , and acute phase proteins ( CRP , alpha 1 antitrypsin ; alpha 1 AT , alpha 1 acid glycoprotein ; alpha 1 AG , fibrinogen ; FBG , pancreatic secretory trypsin inhibitor ; PSTI ) , and the plasma concentration of polymorphonuclear cell elastase ; PMN E and white blood cell counts were measured in 18 patients with esophageal cancer who underwent radical esophagectomy through right thoracotomy and reconstruction with gastric tube . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In this regard , NE plays a central role in the pathogenesis of pulmonary emphysema by destroying the alveolar walls of the lung in the conditions that antiproteases in the lung such as alpha 1 antitrypsin ( alpha 1 AT ) are inactivated e . g . , cigarette smoking , or alpha 1 AT deficiency caused by mutations of the alpha 1 AT gene resulting in excess burden of NE in the lung . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In the pulmonary vein there was a significant increase in neutrophil expressed CD11b ( P < 0 . 001 ) , neutrophil elastase : alpha 1 antitrypsin complexes ( P < 0 . 001 ) , endothelin 1 ( P < 0 . 001 ) and thrombin antithrombin complexes ( P < 0 . 001 ) by the end of bypass compared with pre operative levels . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
BACKGROUND : The protein alpha 1 antitrypsin is a prototype member of the serpin ( serine protease inhibitor ) family and is known to inhibit the activity of neutrophil elastase in the lower respiratory tract . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Based on the most recent available crystal structures and biochemical studies of protease complexes of normal and mutant serine protease inhibitors ( serpins ) , we have built models of the complexes : alpha 1 antitrypsin + human neutrophil elastase ; alpha 1 antitrypsin Pittsburgh ( 358Met > Arg ) ( Scott et al . , J . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin ( alpha 1 AT ) , the major inhibitor of NE , is synthesized by mature neutrophils . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutralization of excess NE by delivering supplemental alpha 1 antitrypsin to the airways via aerosolization represents an exciting new potential therapy for CF lung disease . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Function of a rare variant of alpha 1 antitrypsin , phenotype P ( 1 ) EFranklin S , a poor inhibitor of human neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
On a molar basis , rHME is approximately 30 % as active as human leukocyte elastase in solubilizing elastin . rHME also efficiently degrades alpha 1 antitrypsin ( alpha 1 AT ) , the primary physiological inhibitor of human leukocyte elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Plasma PMN elastase and some acute phase reactants including alpha 1 antitrypsin , alpha 2 macroglobulin , fibrinogen , erythrocyte sedimentation rate ( ESR ) , and transferrin were studied in thirty nine patients with psoriasis , including 16 controlled patients , and forty healthy control subjects . ^^^ PMN elastase was found to correlate significantly with PMN leukocyte count and alpha 1 antitrypsin in active and inactive periods of the disease , but with fibrinogen and psoriasis area and severity index ( PASI ) only in the active period of the disease . ^^^ We conclude that plasma PMN elastase level may be a more specific and sensitive inflammatory marker than alpha 1 antitrypsin , alpha 2 macroglobulin , ESR , and may be a good marker for diagnosis and follow up of the disease activity of the psoriatic patients . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Kinetic characterisation of alpha 1 antitrypsin F as an inhibitor of human neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Levels of plasma HNE bound to alpha 1 antitrypsin ( pHNE alpha1AT ) were measured by ELISA . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In order to study the colonic intraluminal proteinase antiproteinase imbalance under inflammatory conditions , we determined proteolytic activity ( PA ) , alpha 1 antitrypsin and the activities of trypsin , chymotrypsin and neutrophil elastase in feces from patients with ulcerative colitis ( UC ) comparing the results with a control group . ^^^ We found an increase in fecal PA , alpha 1 antitrypsin and neutrophil elastase in patients with UC and the correlation between the severity of the disease and the PA was statistically significant ( r = 0 . 62 , P < 0 . 05 ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The imbalance of neutrophil elastase and alpha 1 antitrypsin in pleural effusion after lobectomy and the effects of the neutrophil elastase inhibitors , sodium N [ 2 [ 4 ( 2 , 2 Dimethylpropionyloxy ) phenyl sulfonylamino ] benzo yl ] aminoacetic acid ( ONO 5046 ) and purified alpha 1 antitrypsin , on neutrophil elastase activity were determined . ^^^ The amount of neutrophil elastase complexed to alpha 1 antitrypsin , measured by an enzyme linked immunosorbent assay , was 170 times higher in pleural effusion than in blood 3 h after lobectomy . ^^^ ONO 5046 , but not alpha 1 antitrypsin , reduced the neutrophil elastase activity in pleural effusion . ^^^ There is an imbalance of neutrophil elastase and alpha 1 antitrypsin in pleural effusion after lobectomy . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The aim of this study was to investigate the extracellular degrading proteolytic cascade proteins referred to as matrix metalloproteinase 1 ( MMP 1 ) , MMP 2 , MMP 9 , membrane type matrix metalloproteinase 1 ( MT 1 MMP ) , tissue inhibitors of matrix metalloproteinase 1 ( TIMP 1 ) , TIMP 2 , neutrophil elastase , and alpha 1 antitrypsin in human pulmonary emphysema . ^^^ Results of the neutrophil elastase as analyzed by EIA , and alpha 1 antitrypsin levels as detected by laser nephelometric immunoassay , indicated no statistical difference between the emphysematous and control groups . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Ilomostat ( galardin ) is a synthetic , specific inhibitor of matrix metalloproteinases including P . aeruginosa elastase and alkaline protease , whereas alpha 1 antitrypsin inhibits serine proteases including neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The cleavage was completely inhibited by alpha 1 antitrypsin , a natural HLE protease inhibitor . ^^^ The cleavage induced by incubation of cells with the sputum sample was totally inhibited by alpha 1 antitrypsin and the specific peptidic HLE inhibitor N methoxysuccinyl Ala Ala Pro Val chloromethylketone . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Deficiency of the serine proteinase inhibitor ( serpin ) alpha 1 antitrypsin ( alpha 1AT ) is the most common autosomal recessive genetic disorder in Northern Europe . alpha 1AT is the physiological regulator of the proteolytic enzyme neutrophil elastase and severe deficiency states are associated with an increased risk of developing chronic obstructive pulmonary disease ( COPD ) as a consequence of chronic proteolytic damage to the lungs . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Human interleukin 1beta ( IL1beta ) was used as a presentation scaffold for the characterization of the reactive site loop ( RSL ) of the serpin alpha 1 antitrypsin ( A1AT ) , the physiological inhibitor of leukocyte elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The present study deals with those of the mediators which have shown demonstrable activity in the course of pancreatitis , e . g . acute phase proteins ( among others C reactive protein and alpha 1 antitrypsin ) and neutrophil elastase ( PMN elastase ) as the marker for granulocyte activity . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
From this point of view , we also examined leukocyte elastase , alpha 1 antitrypsin , and albumin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Further studies in patients with IBD are needed to determine whether other faecal parameters , such as lactoferrin , tumour necrosis factor alpha , PMN elastase , lysozyme , leucocyte esterase , immunoglobulin A , among others , are more accurate or cost effective than measurement of alpha 1 antitrypsin in the stools of such patients . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutrophil elastase ( a 240 aa mature protein predominantly found in neutrophil granules ) is the target for protease inhibition by alpha 1 antitrypsin , and its unopposed release destroys tissue at sites of inflammation . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Oxidation of either methionine 351 or methionine 358 in alpha 1 antitrypsin causes loss of anti neutrophil elastase activity . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
AIMS / BACKGROUND : alpha 1 antitrypsin ( alpha 1 AT ) is a hepatic acute phase protein which predominantly inhibits neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The antiprotease , alpha 1 antitrypsin , is the major circulating serine protease inhibitor which protects tissues from neutrophil elastase attack . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
A variety of genetic disorders and exogenous microbial proteinases disturb the balance between proteinases and cognate inhibitors . alpha 1 Antitrypsin deficiency is a model disorder resulting in an insufficient control of leukocyte elastase with a subsequent alveolar tissue damage . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The inflammatory markers measured were calprotectin , a prominent protein in the cytosol fraction of neutrophil granulocytes , and alpha 1 antitrypsin ( alpha 1 AT ) , the major inhibitor of neutrophil elastase in the lower respiratory system . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In certain pathological circumstances , an imbalance between NE and its major plasmatic inhibitor alpha 1 PI ( formerly , alpha 1 antitrypsin ) leads to abnormal tissue destruction and disease development . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
As a measure for the inflamed enzymatic potence appears the increase of the PMN Elastase Concentration with a relative lack of alpha 1 Antitrypsin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin deficiency in Europe : geographical distribution of Pi types S and Z . alpha 1 Antitrypsin ( AT ) is the principal serum inhibitor of proteolytic enzymes such as neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
This increase in activity was not due an interference with the specific inhibition of HNE by alpha 1 antitrypsin but colistin was found to reverse the inhibitory effects of small molecular weight molecules like heparin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Interleukin ( IL ) 1beta , IL 6 , IL 8 , leukotriene B ( 4 ) ( LTB ( 4 ) ) , secretory leukoprotease inhibitor ( SLPI ) , alpha 1 antitrypsin ( alpha ( 1 ) AT ) , and tumour necrosis factor alpha ( TNFalpha ) were measured by ELISA , and neutrophil elastase ( NE ) and myeloperoxidase ( MPO ) by chromogenic substrate assay . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Modifier genes are implicated for this heterogeneity . alpha 1 antitrypsin ( alpha 1 AT ) is one of the few antiproteases capable of inactivating neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
This action required proteolytic activity as alpha 1 antitrypsin blocked NE induced PGE 2 release . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
This contrasts with the effects of exogenous antielastases on sputum neutrophil elastase activity that of alpha 1 antitrypsin was limited , but that of secretory leukoproteinase inhibitor was facilitated . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Levels and activities of alpha 1 antitrypsin ( A1AT ) , secretory leucoprotease inhibitor ( SLPI ) , and neutrophil elastase ( NE ) were quantified . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
However , serum NE and alpha 1 antitrypsin concentrations were not significantly different between patients and controls , even during acute exacerbation in the former . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Each elastase inactivated the endogenous inhibitor of the other , with neutrophil elastase degrading tissue inhibitor of metalloproteinase 1 , and macrophage elastase degrading alpha 1 antitrypsin . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
BACKGROUND : Alpha 1 antitrypsin ( AAT ) serves primarily as an inhibitor of the elastin degrading proteases , neutrophil elastase and proteinase 3 . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The properties of natural occurring inhibitors of NE such as alpha one antitrypsin and secretory leukoprotease inhibitor are reviewed . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The purpose of this study was to measure the matrix metalloproteinases ( MMP ) and human neutrophil elastase ( HNE ) activities in chronic otitis externa ( COE ) and to determine whether administration of protease inhibitors recombinant alpha 1 antitrypsin ( rAAT ) and ilomastat might reduce these protease activities . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In the subtraction , carboxypeptidase B , trypsinogen , elastase 1 , fibrinogen , elastase 2 , triacyl glycerol lipase , and alpha 1 antitrypsin genes showed lower expression . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Furthermore , NE was suggested as one of the determinant factors of individual susceptibility to lung cancer resulting from imbalance between alpha 1 antitrypsin ( AT ) and NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Although alpha 1 antitrypsin is mainly produced in the liver , its main function is to protect the lung against proteolytic damage from neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 antitrypsin and neutrophil elastase imbalance and lung cancer risk . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
SP A binds alpha 1 antitrypsin in vitro and reduces the association rate constant for neutrophil elastase . ^^^ METHODS AND RESULTS : At an alpha 1 antitrypsin : SP A molar ratio of 1 : 1 , the interaction resulted in a calcium dependent decrease of 84 . 6 % in the association rate constant of alpha 1 antitrypsin for neutrophil elastase . ^^^ CONCLUSION : We conclude that the binding of SP A to alpha 1 antitrypsin results in a decrease of the inhibition of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The conversion of the reactive center bond of the serpin alpha 1 proteinase inhibitor ( alpha 1 PI , also known as alpha 1 antitrypsin ) from Met Ser to Arg Ser decreases the rate at which it inhibits neutrophil elastase and endows it with the ability to inhibit thrombin and activated protein C ( APC ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The in vitro effects of the Pseudomonas aeruginosa derived phenazine pigments pyocyanin and 1 hydroxyphenazine ( 1 hp ) on neutrophil elastase release and myeloperoxidase induced inactivation of alpha 1 protease inhibitor ( alpha 1 PI ) were investigated . 1 hp ( 6 25 microM ) , but not pyocyanin , caused a dose dependent enhancement of elastase release by FMLP : cytochalasin B ( CB ) activated human neutrophils . 1 hp ( 0 . 78 6 . 25 microM ) also increased the oxidative inactivation of the elastase inhibitory capacity of alpha 1 PI exposed to FMLP : CB activated neutrophils . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The major endogenous inhibitor of neutrophil elastase in the plasma , alpha 1 protease inhibitor ( alpha 1 PI ) , has a single cysteine residue which has been shown to form mixed disulfides with a number of thiols in vitro . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
We have studied glycosidases activities , elastase like activity , immunoreactive alpha 1 protease inhibitor ( alpha 1PI ) , neutrophil elastase inhibitory capacity ( NEIC ) and myeloperoxidase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Lung injury in idiopathic pulmonary fibrosis and measurement of immunoreactive neutrophil elastase and alpha 1 protease inhibitor in blood ] . ^^^ In order to examine whether the pathogenesis of IPF involves the proteolytic mechanism of enzymes as in emphysema , concentrations of plasma neutrophil elastase and serum alpha 1 protease inhibitor were measured in patients with IPF , and compared with the levels in patients with emphysema and in normal individuals . ^^^ In some patients with IPF , the blood concentration of neutrophil elastase was much higher than normal and the degree of imbalance between neutrophil elastase and alpha 1 protease inhibitor was significantly great than in patients with emphysema . ^^^ The blood concentration of neutrophil elastase and alpha 1 protease inhibitor ratio may be useful in assessing the degree of lung injury . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Because intravascular coagulation may accompany severe burn injury , this study examined burn wound exudates for PMN elastase and its physiologic inhibitor , plasma alpha 1 protease inhibitor ( alpha 1 PI ) , as a step in evaluating their contributions to coagulopathy in patients with burns . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Reversible inhibition of neutrophil elastase by thiol modified alpha 1 protease inhibitor . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Contribution of additional elastase inhibitors other than alpha 1 protease inhibitor and bronchial antileukoprotease to neutrophil elastase inhibiting capacity of bronchoalveolar lavage . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Thus , sarcoidosis ( mostly lymphocytic ) is associated with enhanced macrophage derived proteolytic activity in BAL , while CVD patients both with and without lung disease have increased neutrophil counts and neutrophil elastase complexed to alpha 1 protease inhibitor and presumably inactive in BAL . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Incubation of 3H rSLPI HNE complex with alpha 1 protease inhibitor for 3 hours at 37 degrees C decreased the amount of complex compared with incubation in the presence of bovine serum albumin ( 70 % vs 27 % dissociated ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
A study was undertaken to determine whether emphysema and airway secretory cell metaplasia , induced in hamsters by intratracheal treatment with human neutrophil elastase ( HNE ) , could be moderated by pretreatment with human alpha 1 protease inhibitor ( API ) . ^^^ Alpha 1 protease inhibitor moderates human neutrophil elastase induced emphysema and secretory cell metaplasia in hamsters . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The two major protease inhibitors in mouse plasma are alpha 1 protease inhibitor ( alpha 1 PI ) , putative inhibitor of neutrophil elastase , and contrapsin , an inhibitor in vitro of trypsinlike proteases . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Endotoxin increased PMN elastase in plasma and lymph whereas the level of alpha 1 protease inhibitor ( alpha 1 PI ) did not change . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The alpha 1 protease inhibitor , or alpha antitrypsin ( AAT ) , a major plasma inhibitor of leukocyte elastase and bacterial proteases , is encoded at the PI locus on chromosome 14 ( 14q24 . 3 q32 . 1 ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutrophil elastase , bound to alpha 1 protease inhibitor ( alpha 1 PI ) , was found to increase from 0 . 47 micrograms / ml in untreated monkeys to 0 . 99 micrograms / ml in FNLP treated animals and 1 . 23 micrograms / ml in monkeys receiving PMA . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Oxidant species produced by human polymorphonuclear leukocytes ( PMN ) inactivate alpha 1 protease inhibitor and thus may indirectly enhance neutrophil elastase induced proteolysis . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Alpha 1 protease inhibitor ( alpha 1 PI ) is the major regulator of extracellular leukocyte elastase activity and can be rendered impotent against elastase by oxidation of a critical methionine , residue 358 . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The source of this fibril bound enzyme activity was consistent with human neutrophil elastase ( HNE ) , since it was readily extracted by high salt solutions and inhibited by an elastase specific chloromethyl ketone inhibitor , human alpha 1 protease inhibitor or by an antibody specific for HNE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The synergistic nature of the interaction between H2O2 and NE mediated injury was further clarified when GO and NE induced lung injury was prevented by addition of an oxidant resistant NE inhibitor ( Eglin C ) , but not an oxidant sensitive NE inhibitor ( human alpha 1 protease inhibitor , alpha 1PI ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Human neutrophil elastase does not bind to alpha 1 protease inhibitor that has been exposed to activated human neutrophils . ^^^ The exposure of alpha 1 protease inhibitor to the myeloperoxidase hydrogen peroxide halide system resulted in a nearly complete loss of its ability to bind and inactivate purified human neutrophil elastase . ^^^ A similar loss of binding to and inactivation of human neutrophil elastase was observed on exposure of alpha 1 protease inhibitor to human neutrophils in the presence of a halide and the neutrophil activating agent , phorbol myristate acetate . ^^^ Thus , the reported effects of leukocyte oxidants , especially the myeloperoxidase system , on alpha 1 protease inhibitor have been confirmed using the most pathophysiologically relevant protease , human neutrophil elastase , as the test enzyme . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Decreased functional alpha 1 protease inhibitor , an inhibitor of neutrophil elastase , might render smokers susceptible to elastase catalyzed destruction of pulmonary elastic fibers and the development of emphysema . ^^^ Binding and inactivation of isotopically labeled porcine pancreatic elastase and human neutrophil elastase by alpha 1 protease inhibitor were measured in fluid obtained by bronchoalveolar lavage of volunteers . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The effect of solid phase fibrin on the inactivation of plasmin , miniplasmin , and neutrophil leukocyte elastase ( PMN elastase ) by plasma protease inhibitors ( alpha 2 antiplasmin , alpha 1 protease inhibitor , alpha 2 macroglobulin ) was studied . ^^^ In Hanks ' balanced salt solution , fibrin reduces the second order rate constant for the inhibition of PMN elastase by alpha 1 protease inhibitor from 8 , 760 10 10 ( 4 ) to 4 10 10 ( 4 ) M 1 . s 1 and by alpha 2 macroglobulin from 121 10 10 ( 4 ) to 1 . 8 10 10 ( 4 ) M 1 . s 1 . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In the case of PMN elastase , heparin ( 300 nM ) further increases enzyme protection against alpha 1 protease inhibitor ; the rate constant decreases from 41 10 10 ( 3 ) M 1 s 1 to 23 10 10 ( 3 ) M 1 s 1 . alpha 2 Macroglobulin inhibits fibrin bound miniplasmin and PMN elastase with a second order rate constant of 1 . 8 10 10 ( 4 ) M 1 s 1 and heparin ( 300 nM ) increases the rate insignificantly for miniplasmin and by a factor of two for PMN elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Several structurally and functionally different antiproteases , alpha 1 protease inhibitor , soybean trypsin inhibitor , two chloromethyl ketone derivatives ( N tosyl L lysine chloromethyl ketone and methoxysuccinyl Ala Ala Pro Val chloromethyl ketone ) , and L 658 , 758 , a neutrophil elastase inhibitor , attenuated the release of neutrophil chemotactic activity and lactate dehydrogenase ( P < 0 . 01 ) . alpha 1 Protease inhibitor and N tosyl L lysine chloromethyl ketone attenuated the inhibition of BEC accumulation by endotoxin ( P < 0 . 001 ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The immunologic NE complex with alpha 1 protease inhibitor ( alpha 1 PI ) was released significantly higher in the LAA ( + ) group than in the LAA ( ) group ( 17 . 4 + / 6 . 5 versus 1 . 8 + / 0 . 6 micrograms / L , respectively , p < 0 . 05 ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Despite the homology with alpha 1 protease inhibitor ( alpha 1PI ) , wild type antichymotrypsin ( ACT ) is a substrate for HNE rather than an inhibitor of the enzyme . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Several studies have shown increased plasma concentrations of neutrophil elastase in complex with alpha 1 protease inhibitor and / or lactoferrin in inflammatory conditions , and serial measurements have been advocated in order to follow disease activity . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In an attempt to further evaluate the role of neutrophil elastase ( NE ) in the development of emphysema , we examined the immunologic quantity of NE bound to alpha 1 protease inhibitor ( PI ) , the NE inhibitory activity , and the molecular pattern of alpha 1 PI in unconcentrated bronchoalveolar lavage fluid ( BALF ) supernatant from 36 community based older volunteers . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Only two of the proteins were efficient inhibitors of neutrophil elastase , the major physiological target of the sole human alpha 1 protease inhibitor ( antitrypsin ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Plasma was analysed for neutrophil elastase , interleukin ( IL ) 8 and neutrophil elastase in complex with alpha 1 protease inhibitor ( alpha 1PI ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
No effects were observed with heat inactivated PMN elastase ( 95 degrees C , 30 min ) or with PMN elastase in the presence of human alpha 1 protease inhibitor ( 2 micrograms / microgram elastase ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The octapeptide Val 89 Thr Val Ala Pro Val His Ile 96 ( CRP 89 96 ) is shown to inhibit hLE and hCG to a larger extent than peptides of similar chain lengths corresponding to the active sites of their natural inhibitors , alpha 1 protease inhibitor and alpha antichymotrypsin , respectively . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
METHODS : We measured the ir NE concentration in 144 non small cell lung cancer tumor extracts using EIA , which permits the rapid measurement of both the free and alpha 1 protease inhibitor ( alpha 1 PI ) complexed form of ir NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Tracheal mucous velocity CTMV ) , an index of mucociliary function , was measured with a roentgenographic technique before and serially after airway challenge with Ascaris suum antigen alone , or after pretreatment with aerosolized alpha 1 protease inhibitor ( alpha 1 PI , 10 mg ) or the specific neutrophil elastase inhibitor ICI 200 , 355 ( 10 mg ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Secretory leukoprotease inhibitor ( SLPI ) and alpha 1 protease inhibitor ( alpha 1 PI ) are powerful antiproteases currently under investigation for their potential to protect the lung from neutrophil elastase ( NE ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Three types of endogenous protease inhibitors control the activity of neutrophil elastase , including alpha 1 protease inhibitor ( alpha 1PI ) , alpha 2 macroglobulin and secreted leukoproteinase inhibitor ( SLPI ) [ 8 10 ] . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
We utilized a reactive loop switching strategy to determine the extent to which the inhibitory activity of alpha 1 protease inhibitor ( PI ) against human neutrophil elastase ( HNE ) could be transferred to alpha 1 antichymotrypsin ( ACT ) , a serpin that does not inhibit HNE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Furthermore , reactive oxygen species are known to inactivate the alpha 1 protease inhibitor ( alpha 1 PI ) , a potent neutrophil elastase inhibitor in plasma . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The protease antiprotease balance was studied by measuring immunoreactive anionic trypsin ( irAT ) , cationic trypsin ( irCT ) , complexes between cationic trypsin and alpha 1 protease inhibitor ( irCT alpha 1 PI ) , leukocyte elastase and neutrophil proteinase 4 ( NP 4 ) , as well as the endogenous protease inhibitors , pancreatic secretory trypsin inhibitor ( PSTI ) , alpha 2 macroglobulin ( alpha 2M ) , alpha 1 protease inhibitor ( alpha 1 PI ) , antichymotrypsin ( ACHY ) , and secretory leukocyte protease inhibitor ( SLPI ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
This study examined the role of cysteine proteinases and their inhibitor in the development of emphysema in comparison with neutrophil elastase ( NE ) complexed with alpha 1 protease inhibitor ( NE alpha 1 PI ) , which was previously demonstrated to be increased in bronchoalveolar lavage ( BAL ) fluid from subjects with subclinical emphysema . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Interleukin 8 ( IL 8 ) , tumor necrosis factor alpha ( TNF alpha ) , neutrophil elastase alpha 1 protease inhibitor complex ( NE complex ) , protein , and alpha 1 protease inhibitor ( alpha 1 PI ) were measured in serum and sputum collected from CF patients during respiratory exacerbations and periods of well being . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
This was not due to cell proliferation or to degradation to soluble collagen fragments because the amounts of DNA and hydroxyproline were not altered . alpha 1 Protease inhibitor and the synthetic inhibitor of NE , L 680 , 833 , when added in sufficient amount to inhibit free elastase activity , blocked the contraction induced by NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Thus , control of the level of such vascular mediators appears important both in infectious disease and in cancer . alpha 1 Protease inhibitor , which inhibits neutrophil elastase , is inactivated by oxidative metabolites such as superoxide and peroxynitrite , and this effect activates matrix metalloproteinases . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Hyperproliferative effects of human leukocyte elastase in vitro and in vivo were abolished by the addition of elastase inhibitors , such as elafin , anti leukoprotease , and alpha 1 protease inhibitor . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The hydrolysis of peptide substrates reflects the specificity of the parent serpin including those from alpha 1 protease inhibitor and monocyte neutrophil elastase inhibitor , two potent inhibitors of elastase and Pr 3 . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Because neutrophil elastase appears to be a key mediator of tissue damage in CF lung disease , anti elastase compounds have also been studied , including alpha 1 protease inhibitor , secretory leukocyte protease inhibitor , and small molecule inhibitors . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Neutrophil elastase ( NE ) cleaves coagulation factors ; thus , elevated elastase levels or its dysregulation by alpha 1 protease inhibitor ( Alpha1PI ) may be linked to DIC . ^^^ Neutrophil elastase ( NE ) cleaves coagulation factors ; thus , elevated elastase levels or its dysregulation by alpha 1 protease inhibitor ( Alpha1PI ) may be linked to DIC . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
We have used a new fluorogenic neutrophil elastase substrate to measure the activity of free and membrane bound human neutrophil elastase ( HNE ) in the presence of alpha 1 protease inhibitor ( alpha 1 Pi ) , the main physiological inhibitor of neutrophil serine proteases in lung secretions . ^^^ Inhibition of neutrophil elastase by alpha 1 protease inhibitor at the surface of human polymorphonuclear neutrophils . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
However , it had no significant effects on norepinephrine ( NE ) stimulated [ 3H ] IP 1 formation or alpha 1A adrenoceptor subtype . ^^^ The lack of effects on NE stimulated PI turnover in ECS treated rats may be due to its lack of effect on the alpha 1A adrenoceptor subtype . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
L 1 , after which only alpha 1A adrenoceptors were left , reduced the vascular contraction induced by NE to 36 % of the control ( P < 0 . 01 ) . ^^^ Compared with alpha 1B subtype , alpha 1A subtype has a lower affinity but a more reserve and a higher efficacy for NE . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In the aortae and mesenteric arteries from old rats , the blocking effect of chlorethylclonidine ( CEC , selective alpha 1b subtype antagonist ) on NE induced contraction was decreased significantly , while the effect of nifedipine ( selectively blocks the response of alpha 1a subtype ) was increased . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Both chlorethylclonidine , an irreversible antagonist of alpha 1b adrenoceptors , and 5 methylurapidil , an alpha 1a selective antagonist , reduced NE induced IP formation regardless of the hormonal condition of the animals . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Vasoconstrictive responses to norepinephrine ( NE ) in isolated rat aorta and renal artery were respectively taken to represent the biological responses mediated by alpha 1B and alpha 1A subtype adrenoceptor ( AR ) . ^^^ After blood vessels were incubated with 10 mumol / L NE for 2 h , alpha 1 AR mediated vasocontraction was desensitized significantly , alpha 1B AR being more marked than alpha 1A AR . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The NE induced positive chronotropic response was inhibited 68 % by the alpha 1B adrenoceptor antagonist , chloroethylclonidine ( CEC ) , but partially ( 41 % ) so by the alpha 1A adrenoceptor antagonist , WB 4101 . ^^^ These findings indicate ( 1 ) that cultured neonatal rat cardiac myocytes possess both alpha 1 adrenoceptor subtypes , i . e . , alpha 1A and alpha 1B , ( 2 ) that the predominant alpha 1 adrenoceptor subtypes mediating NE induced positive chronotropy in neonatal rat cardiac myocytes at day 3 of culture are alpha 1B subtypes , and ( 3 ) that NE induced positive chronotropy may be caused via voltage gated Ca2+ channel activation . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
These results indicate ( 1 ) that cultured neonatal rat cardiac myocytes contain both alpha 1 adrenoceptor subtypes , i . e . , alpha 1A and alpha 1B , and ( 2 ) that the predominant alpha 1 adrenoceptor subtypes mediating NE induced chronotropy were altered by hypoxia . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Pretreatment of astrocytes with 100 microM CEC , an alpha 1B adrenoceptor specific , irreversible antagonist increased the EC 50 values for NE induced InsPn accumulation in non treated as well as in dBcAMP treated cultures , indicating that both the alpha 1A and alpha 1B adrenoceptor subtypes were expressed under both culturing conditions . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
These studies evaluated the effect of a selective alpha 1D adrenoceptor antagonist , BMY 7378 ( 8 [ 2 [ 4 ( 2 methoxyphenyl ) 1 piperazinyl ] ethyl ] 8 azaspirol [ 4 , 5 ] decane 7 , 9 dio ne 2HCl ) , on the pressor effect to i . v . phenylephrine ( alpha 1A / B / D adrenoceptor agonist ) and ( R ) A 61603 ( alpha 1A adrenoceptor agonist ; N [ 5 ( 4 , 5 dihydro 1 H imidazol 2yl ) 2 hydroxy 5 , 6 , 7 , 8 tetrahydronaphthalen l yl ] methanesulfonamide HBr ) in pithed rats . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Expression of human alpha 1a , alpha 1b , and alpha 1d AR cDNAs under the repressible control of anhydrotetracycline in human embryonic kidney ( HEK ) 293 cells , which normally express no alpha 1 ARs , was used to compare responses to norepinephrine ( NE ) at different receptor densities . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The present study examined whether hypoxia / reoxygenation ( H / R ) attenuates norepinephrine ( NE ) effectiveness in small arteries by interfering with function of alpha 1a and / or alpha 1b adrenoceptor subtypes . ^^^ In other groups , alpha 1b receptors were blocked with chloroethylclonidine and alpha 1a receptors were blocked with 5 methylurapidil or WB 4101 to produce exclusive alpha 1a or alpha 1b responses to NE . ^^^ SOD and CAT prevented the H / R induced contractile dysfunction . alpha 1a Receptor responses to NE were not altered by H / R . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
METHODS AND RESULTS : We used standard microelectrode techniques to study the concentration dependent actions of three alpha 1 adrenoreceptor blockers ( WB [ alpha 1A > or = alpha 1D ] , 5 methylurapidil [ 5 MU ] [ alpha 1A > > alpha 1D ] , and UK 52 , 046 [ nonselective ] ) or norepinephrine ( NE ) effects in normal PF and in PF depolarized with a simulated ischemic solution ( [ K+ ] o = 10 mM ; pO 2 < 20 mmHg ; pH 6 . 8 ; maximum diastolic potential 60 + / 1 mV ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In intact rats , arterial [ NE ] ranged between 0 . 1 and 15 ng / microgram DNA , arterial [ alpha 1 AR ] ranged between 12 . 4 and 46 . 8 fmol / mg protein and [ alpha 1A AR ] ranged between 0 . 05 and 27 . 9 fmol / mg protein . ^^^ However , with respect to the [ alpha 1A AR ] a significant correlation between [ NE ] and [ alpha 1A AR ] was observed . ^^^ Two weeks after chemical sympathectomy of the rats with 6 hydroxydopamine ( 1 ) arterial [ NE ] was markedly reduced , and ( 2 ) a distinct reduction in the [ alpha 1A AR ] as percentage of the total [ alpha 1 AR ] density in mesenteric artery side branches was noted . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
These results indicate that both alpha 1A and alpha 1D subtypes are functionally up regulated in SHR muscle vascular bed where the ascending limb of VO 2 is predominantly mediated by the alpha 1D at a much lower concentration for NE than the descending limb which is predominantly mediated by the alpha 1A subtype . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The desensitization of alpha 1A AR took place earliest and needed lowest concentration of NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In the 12 month old rats , compared with the 10 week old rats , ( 1 ) the maximal contraction induced by norepinephrine ( NE ) was reduced , without significant alteration of pD 2 value ; ( 2 ) the inhibitory effect of chlorethylclonidine ( an irreversible antagonist for alpha 1B and alpha 1D subtype ) on NE induced contraction was weaker ; ( 3 ) using NE as an agonist , the pA 2 value for WB 4101 ( an alpha 1A and alpha 1D selective antagonist ) was not changed , but the pA 2 value for BMY 7378 ( an alpha 1D selective antagonist ) was decreased , while the pA 2 value for sertindole ( an alpha 1A selective antagonist ) was increased . ^^^ Thus , differently from the 10 week old rats in which only alpha 1D adrenoceptors mediate NE induced contraction in aortae , both alpha 1A and alpha 1D adrenoceptors ( mainly alpha 1A ) mediate the response in the 12 month old rats . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
In PC 12 cells stably transfected with the human alpha 1A AR , norepinephrine ( NE ) strongly activated both extracellular signal regulated kinases ( ERKs ) and c jun NH 2 terminal kinases ( JNK ) . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NE stimulated activation of extracellular signal regulated kinases ( ERKs ) in cells expressing all three alpha 1 AR subtypes , although alpha1A and alpha1B ARs caused larger ERK activation than did alpha1D ARs . ^^^ NE also activated p 38 MAPK in alpha1A and alpha1B but not alpha1D transfected cells and activated c Jun NH 2 terminal kinase ( JNK ) only in alpha1A transfected cells . ^^^ NE , but not NGF , strongly stimulated tyrosine phosphorylation of a 70 kDa protein only in alpha1A transfected PC 12 cells . ^^^ NE caused neurite outgrowth only in alpha1A expressing PC 12 cells , but not in alpha1B or alpha1D transfected cells , whereas NGF caused neurite outgrowth in all cells . ^^^ Only the alpha1A AR expressing cells differentiated in response to NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Treatment with norepinephrine ( NE ) in the presence of propranolol for 24 h increased DNA synthesis in HEK293 / alpha 1A or HEK293 / alpha 1B AR cells concentration dependently , with EC 50 values of 48 . 8 nmol . ^^^ Preincubation with prazosin completely abolished NE induced CCDPK activation in HEK293 / alpha 1A and alpha 1B AR cells . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NE also activated luciferase reporter constructs for seven different transcription factors ( AP 1 , SRE , CRE , NFkappaB , NFAT , Stat , GAS ) following transfection into alpha1A AR expressing PC 12 cells . ^^^ NE caused differentiation of alpha1A , but not alpha1B or alpha1D , AR expressing PC 12 cells similar to that caused by NGF . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The pKI values for DDPH in tissues and the cloned alpha 1A or alpha 1D AR were shown to fit well in with the pA 2 values in antagonizing NE induced constriction in rat isolated aorta , renal artery and spleen . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Nerve released NE acts primarily at alpha1B and to a lesser extent at alpha1A ARs in SHAM arteries whereas NE mediates neurogenic constrictions in DOCA salt arteries by acting at alpha1B ARs . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
That this activity was due to alpha 2 macroglobulin ( A2MG ) complexed neutrophil elastase was evidenced by ( a ) the Sephadex G 75 elution profile ; ( b ) the inactivity against insoluble [ 3H ] elastin ; ( c ) the inhibitory profile with phenylmethylsulfonyl fluoride , methoxy succinyl alanyl alanyl prolyl valyl chloromethylketone , ethylene diamine tetraacetic acid , and A1AT ; and ( d ) the immobilization by A2MG antibody bound to polystyrene plates . ^^^ Furthermore , in agreement with the predicted affinity of A1AT and A2MG for neutrophil elastase , the ratio of A2MG to A1AT in the fluid ( 0 . 57 % ) coincided with the ratio of the A2MG to A1AT complexed elastase ( 0 . 36 % ) . ^^^ These findings suggest that the net lung protease antiprotease balance in ARDS is shifted largely in favor of the antiproteases ( chiefly A1AT ) , and that the antiproteases , A1AT and A2MG , have similar affinities for neutrophil elastase in vivo . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The major function of A1AT is to inhibit neutrophil elastase ; A1AT does so through an active site centered around Met 358 contained within an external stressed loop on the surface of the molecule . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
A1AT is the principal inhibitor of neutrophil elastase , such that a deficiency of A1AT results in insufficient anti elastase protection in the lower respiratory tract , thus allowing neutrophil elastase to destroy alveolar structures . ^^^ The goal of A1AT augmentation therapy in A1AT deficiency is to raise lung A1AT levels and anti neutrophil elastase capacity to levels that will provide adequate protection against neutrophil elastase , thereby preventing the lung from further elastase mediated degradation . ^^^ Of critical importance is the fact that the A1AT that diffused into the lung was active as an inhibitor or neutrophil elastase , resulting in significant augmentation of epithelial lining fluid anti neutrophil elastase capacity and normalization of the lung anti elastase protection . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Attention is focused on the balance between destructive enzymes such as neutrophil elastase and protective proteins such as A1AT , secretory leucocyte proteinase inhibitor ( SLPI ) , human elastase inhibitor ( HEI ) and elafin . ^^^ When neutrophil elastase is already attached to the elastin fibres the smaller molecules SLPI and elafin appear to be better inhibitors of this enzyme than larger inhibitors such as A1AT and HEI . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
A recombinant fusion protein was constructed consisting of an antihuman pIgR single chain Fv ( scFv ) antibody linked to human alpha ( 1 ) antitrypsin ( A1AT ) , an inhibitor of NE . ^^^ The recombinant scFv A1AT fusion protein bound specifically to the pIgR on the basolateral surface of an epithelial cell monolayer , and was transported and released into the apical medium where the A1AT domain was capable of forming an inactivation complex with NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Percentage neutrophil elastase ( NE ) inhibitory capacity of BAL fluid was low in both A1AT deficient subjects and a cigarette smoking normal subject . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Both AAT and placental SPAAT also bind to diisopropyl fluorophosphate ( DFP ) treated HNE as well as cathepsin G . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The development of emphysema in the hereditary PiZ AAT deficiency state suggests that inhibition of leukocyte elastase in the lung is a major function of this protein . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
One hundred percent inhibition of NE by pure AAT and SLPI standards occurred at molar ratios of 0 . 91 + / 0 . 03 for AAT to NE and 0 . 83 + / 0 . 02 for SLPI to NE when assayed in buffer with < or = 0 . 15 mol NaCl / L , compared to ratios of 0 . 99 + / 0 . 02 and 1 . 06 + / 0 . 02 , respectively , for assays in buffers with 0 . 50 1 . 00 mol NaCl / L ( p < 0 . 05 for AAT to NE ; p < 0 . 02 for SLPI to NE ) . ^^^ Assays in buffer with < or = 0 . 15 mol NaCl / L indicated that 86 . 9 + / 4 . 1 % of AAT and 100 . 9 + / 4 . 9 % of SLPI in BALF were active against NE , while assays in buffer with 0 . 50 1 . 00 mol NaCl / L showed that 84 . 4 + / 3 . 5 % of AAT and 81 . 6 + / 5 . 9 % of SLPI present were active . ^^^ AAT inhibited NE and PE equally only in buffer with 0 . 50 1 . 00 mol NaCl / L . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
AAT deficiency results in loss of protection in the lung against neutrophil elastase ( NE ) the major target for AAT . ^^^ The disease process is exacerbated by cigarette smoke , which is capable of oxidizing a critical methionine residue at the active site , rendering AAT an inefficient inhibitor of NE . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The protection of bodies ' AAT shield from serpinolytic activity of collagenase would result in inhibition of serine proteinases such as neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
AAT is prominent among the serine proteinase inhibitors and is an important regulator of leukocyte elastase and proteinase 3 . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
These findings raise the possibilities that protective levels of AAT in the lungs are insufficient or that elastin degradation in the lungs of these subjects is not dependent upon neutrophil elastase at this time . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Excess neutrophil elastase is an important determinant of pulmonary disease in CF . alpha 1 antitrypsin ( AAT ) , also known as alpha 1 proteinase inhibitor ( alpha1PI ) is a major modulator of elastase activity . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Then , the animals were sacrificed , and bronchoalveolar lavage fluid ( BALF ) was collected for measurement of concentrations of total protein ( TP ) , interleukin 8 ( IL 8 ) , tumor necrosis factor ( TNF alpha , the activities of NE and AAT , total phospholipids ( TPL ) and disaturated phosphatidylcholine ( DSPC ) . ^^^ In the BALF , the activity of AAT , TPL , DSPC / TPL were lower than those in Group NS ( P < 0 . 05 ) , but the concentrations of albumin , IL 8 , TNF alpha , the activity of NE and the ratio of W / D were higher than those in Group NS ( P < 0 . 05 ) . ^^^ Those beneficial effects of AAT might be due , in part , to reduction in the levels of mediators that could activate neutrophils , in addition to the direct inhibitory effect on neutrophil elastase . . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Deficiency of AAT is a recognised risk factor for chronic obstructive pulmonary disease ( COPD ) , attributed to uninhibited neutrophil elastase released into the lung tissue during inflammatory states . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Emphysema is caused by the protease antiprotease imbalance when smoking induced release of neutrophil elastase in the lung is inadequately inhibited by the deficient levels of AAT , the major inhibitor of neutrophil elastase . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
Sputum myeloperoxidase ( MPO ) , interleukin 8 , tumor necrosis factor receptors , sputum and plasma NE / AAT complexes , and safety parameters were also measured . ^^^ Sputum NE / AAT complex and MPO levels were lower on rAAT compared to placebo . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
The main physiological role of AAT is to inhibit neutrophil elastase ; however , elastase was efficiently inhibited by AAT in the presence and absence of YopM , indicating that YopM does not interfere with the anti protease inhibition activity of AAT , and that the domain of AAT interacting with YopM is not identical to AAT ' s protease interaction domain . ^^^
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
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Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA
Interacting proteins: P01009 and P08246	Pubmed	SVM Score :0.0
NA