| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| In this study the action of fructose 2 , 6 diphosphate ( F 2 , 6DP ) and other bisphosphorylated sugars which purportedly activate phosphofructokinase 1 ( PFK 1 ; EC 2 . 7 . 1 . 11 ) were studied . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Glucose uptake by the islets , their insulin content , and both the Vmax and the apparent Km for fructose 6 phosphate of phosphofructokinase 1 ( EC 2 . 7 . 1 . 11 ) in PTH treated rats were not different from normal . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The strong enhancement of FDP / G6P ratio is taken as evidence for in vivo stimulation of phosphofructokinase 1 ( PFK ) ( ATP : D fructose 6 phosphate 1 phosphotransferase ; EC 2 . 7 . 1 . 11 ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Mutants lacking Escherichia coli phosphofructokinase ( pfkA , 78 min ) are suppressed by the unlinked pfkB 1 mutation , which restores some enzyme activity ( Morrissey and Fraenkel , 1972 ) . ^^^ We here describe a secondary mutation at pfkB , `` PFKB , ' ' which abolishes the suppression as well as the low residual activity of unsuppressed pfkA mutants . pfkB is at about 33 min . with the gene order groD pps pheS pfkB . ^^^ Strains carrying pfkB or pfkC , but wild type at pfkA , were not markedly affected in growth on sugars . ^^^ A new search for suppressors such as pfkB 1 gave five independent candidates , all of which suppressed both pfkA 1 and pfkA 2 and occurred in the pfkB region ; none occurred at pfkC . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The deduced amino acid sequence of the fruK product exhibits little similarity to the major 6 phosphofructokinase of E . coli ( pfkA ) and the 6 phosphofructokinases present in a number of pro and eukaryotic organisms , but there is 27 % , 25 % and 22 % identify of sequence respectively with the minor 6 phosphofructokinase ( pfkB ) of E . coli , the lacC gene product of Staphylococcus aureus and the ribokinase of E . coli . . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The pfkB gene of Escherichia coli is known to specify a minor phosphofructokinase , Pfk 2 , in the wild type strain ; the pfkB 1 mutation causes a 25 fold increase in the amount of Pfk 2 so that it adequately substitutes for mutational loss of the major phosphofructokinase , Pfk 1 ( specified by pfkA ) ; and another closely linked mutation , pfkB 10 , affects the structure of Pfk 2 . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The glycolytic isozymes pyruvate kinase F and A ( encoded by pykF and pykA , respectively ) and phosphofructokinase 1 and 2 ( pfkA and pfkB , respectively ) exhibited differential regulation via csrA . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| PfkA locus of Escherichia coli . pfkA was know , on the basis of three mutants , as the likely locus of phosphofructokinase in Escherichia coli , and the unlinked pfkB 1 mutation suppressed these mutations by restoring some enzyme activity ( Morrissey and Fraenkel , 1972 ) . ^^^ We now report a new search for the complete inactivation of pfkA ( e . g . , by deletion or amber mutation ) , done to assess whether the pfkB 1 suppression is by an independent enzyme , phosphofructokinase activity 2 ( Fraenkel , Kotlarz , and Buc , 1973 ) . ^^^ Ten new phosphofructokinase mutants all were at pfkA , rather than at pfkB or pfkC . ^^^ All ( including pfkA 11 ) were suppressed by the pfkB 1 mutation . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Chem . 248 , 4865 4866 ) in strains carrying the pfkB 1 mutation , a suppressor of pfkA mutants , and very low levels of this enzyme have also been detected in strains not carrying the suppressor ( i . e . pfkB+ ) . ^^^ The nonallosteric protein has now been prepared pure from three strains , one carrying pfkB 1 and pfkA+ , one carrying pfkB 1 and completely deleted for pfkA , and one carrying pfkB+ and also deleted for pfkA . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| This prediction is tested in Saccharomyces cerevisiae through the use of a high copy plasmid that bears one of the following : hexokinase B ( HEXB ) , phosphoglucose isomerase ( PGI ) , phosphofructokinase ( PFKA and PFKB ) , or pyruvate kinase ( PYK ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Proteins of the PfkB family did not exhibit statistically significant sequence identity with PfkA of E . coli . ^^^ On the basis of their similar functions , sizes , predicted secondary structures , and sequences , we suggest that the PfkA and PfkB families share a common evolutionary origin . . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The anaerobic expression of pfl is reduced both in a strain mutated in the pgi gene and in a pfkA pfkB double mutant strain when cells are grown in medium supplemented with glucose . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Over this time period , insulin provoked selective increases ( up to 5 fold ) in factors relevant to the control of glycolysis : the activities of phosphofructokinase 1 ( PFK 1 ) , phosphofructokinase 2 ( PFK 2 ) and hexokinase isoenzymes and the content of fructose 2 , 6 bisphosphate ( F26BP ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The rat PFK A amino acid sequence is 69 % and 68 % identical to those for rat PFK B and rat PFK C , respectively , and differences in residues involved in binding of allosteric effectors were observed among the three isoforms . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The mouse PFK C gene organization is similar to that of the human and rabbit PFK A and human and mouse PFK B genes . ^^^ Reverse transcription PCR analysis revealed that PFK A , PFK B and PFK C genes were expressed , in all mouse tissues tested , at varying levels . ^^^ PFK A mRNA was more abundantly expressed in all tissues than were the PFK B and PFK C genes . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| PFK activity was insensitive to bacterial PFK A ( allosteric ) effectors such as PEP , implying that Synechocystis PFK should be classified as a PFK B ( non allosteric ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| A plasmid pSDK 1 containing the Escherichia coli phosphofructokinase 1 gene ( pfkA ) was constructed , and transferred into Acidithiobacillus thiooxidans Tt 7 by conjugation . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Construction of an inducible , pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources . ^^^ In addition to having both pfkA and pfkB deleted , RL 257 contains the lacI ( q ) allele , which allows for inducible expression when coupled with an expression vector containing either the lac or tac promoter . . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| To elucidate the precise regulation of glucose homeostasis in chicken skeletal muscle , expression of muscle and liver type phosphofructokinase 1 ( EC : 2 . 7 . 1 . 11 , PFK M , PFK L ) was characterized in the insulin stimulated state by Real Time PCR . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| A second muscle biopsy , performed at 21 months of age , a few days before her death , and postmortem study of heart and liver , disclosed excessive extralysosomal glycogen storage and reduced phosphofructokinase 1 ( PFK 1 ) activity . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The model is applied to the study of three PFK of Escherichia coli : two isoenzymes , phosphofructokinase 1 ( PFK 1 ) and phosphofructokinase 2 ( PFK 2 ) , and a mutant form of phosphofructokinase 2 ( PFK 2 * ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Phosphofructokinase 1 ( PFK 1 ) from Thermus thermophilus differs from other bacterial PFKs in that it is regulated by effector induced reversible tetramer dimer conversion rather than conformational change alone . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Phosphofructokinase 1 ( PFK 1 ) is the enzyme that facilitates the conversion of fructose 6 phosphate to fructose 1 , 2 bisphosphate , a step in the glycolytic pathway that precedes the production of triose phosphates . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The birds with enlarged livers ( high index ) also possessed metabolic abnormalities in that 6 phosphofructokinase ( EC 2 . 7 . 1 . 11 ; PFK 1 ) activity ( measured at low substrate concentration ) was depressed despite the presence of normal , or even slightly elevated fructose 2 , 6 bisphosphate concentration . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Enzyme activities of phosphofructokinase 1 ( PFK 1 ) from glycolysis , alpha ketoglutarate dehydrogenase ( alpha KGDH ) from the Krebs cycle and carnitine palmitoyltransferase ( CPT 1 and 2 ) from fatty acid metabolism in soleus , tibialis anterior and gastrocnemius muscles were measured in trained and sedentary rats . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Phosphofructokinase 1 ( PFK 1 ) and phosphofructokinase 2 ( PFK 2 ) activities were enhanced in cells from diabetic rats compared with those from normal rats . [ U 14C ] glucose incorporation into glycogen was also increased in cells from diabetic rats and the 14CO2 liberation was lesser than in cells from normal animals . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| METHOD : The ATP level and the activities of hexokinase ( HK ) , phosphofructokinase 1 ( PFK 1 ) and pyruvate kinase ( PK ) in red blood cells ( RBC ) were measured in 50 school students 6 12 yr of age in the Jordan Valley ( JV ) which is the lowest region below sea level in the world ( low altitude : 390 m below sea level , hyperoxic and with an average daily value of 796 mmHg barometric pressure ) and in 50 school students of the same age group in Irbid region ( normal altitude : 600 m above sea level and with an average daily value of 600 mmHg barometric pressure ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| A small increase in phosphofructokinase 1 ( PFK 1 ) activity was also detected . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Glucose ( from 5 to 25 mM ) induced phosphofructokinase 1 ( PFK 1 ) isoform C , glyceraldehyde 3 phosphate dehydrogenase ( GAPDH ) ( 4 fold ) , and L pyruvate kinase ( L PK ) ( 7 fold ) mRNAs . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Phosphofructokinase 1 from Saccharomyces cerevisiae is an octameric enzyme comprising two non identical subunits , alpha and beta , which are encoded by the unlinked genes PFK 1 and PFK 2 . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The expression of one of the phosphofructokinase genes ( pfkB ) , however , was repressed by glucose while both PFK 1 and PFK 2 were induced in S . cerevisiae . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Phosphofructokinase 1 ( Pfk 1 ) from Schizosaccharomyces pombe was purified by 54 fold enrichment to homogeneity elaborating the following steps : ( a ) Disruption of the cells with glass beads ; ( b ) fractionated precipitation with polyethylene glycol 6000 ; ( c ) affinity chromatography on Cibacron Blue F3G A Sephadex G 100 ; ( d ) ion exchange chromatography on Resource Q . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Scallop 6 phosphofructo 1 kinase ( EC 2 . 7 . 1 . 11 ) ( PFK 1 ) was strongly activated by 1 microM Fru 2 , 6 BP when 0 . 2 mM Fru 6 P was used as a substrate , but the activity was not affected at 5 mM Fru 6 P . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| PCR amplified hypervariable 16S rDNA fragments of the bacterium Escherichia coli DF 1020 and cDNA of the 6 phosphofructo 1 kinase isoenzymes ( PFK 1 , EC 2 . 7 . 1 . 11 ) in rat brain were chosen as model systems for essentially heterogeneous templates . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The following enzymes of the carbohydrate metabolism , hexokinase ( HK ) , phosphofructokinase 1 ( PFK 1 ) , pyruvate kinase ( PK ) , glucose 6 phosphate dehydrogenase ( G6PD ) , and lactate dehydrogenase ( LDH ) as well as the content of glycogen were determined in the salivary glands of rats aged 2 , 7 , 14 , 21 , 30 and 60 days . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Random peptide phage display analysis revealed a consensus motif ( WLELRP ) with almost complete homology to part of the enzyme phosphofructokinase 1 ( PFK 1 ) . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Pyrophosphate dependent phosphofructokinase ( PFP ; EC 2 . 7 . 1 . 90 ) and two isoforms of ATP dependent phosphofructokinase ( PFK 1 and PFK 2 ; EC 2 . 7 . 1 . 11 ) from ripened banana ( Musa cavendishii L . cv . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Phosphofructokinase 1 ( PFK 1 ) was 16 % decreased in HTG hearts , whereas pyruvate kinase activity did not change . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| To elucidate the specificity of glucose metabolism in chicken skeletal muscle , changes in mRNA levels of hexokinase 1 ( HKI ) , hexokinase 2 ( HKII ) , phosphofructokinase 1 ( PFK 1 ) and glycogen synthase ( GS ) were characterized in acute and persistent hypoglycemia induced by tolbutamide administration . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Determination of the maximal velocity ( 5 ( max ) ) of the 10 glycolytic enzymes from hexokinase to lactate dehydrogenase revealed that hexokinase ( 153 306 times ) and phosphofructokinase 1 ( PFK 1 ) ( 22 56 times ) had higher over expression in rat AS 30D hepatoma cells than in normal freshly isolated rat hepatocytes . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Because fructose 2 , 6 diphosphate is a product of phosphofructokinase 2 ( PFK 2 ) , these data suggest that AMPK regulates post mortem glycolysis through its phosphorylation and activation of PFK 2 , which then up regulates the activity of phosphofructokinase 1 ( PFK 1 ) , a key rate controlling enzyme in glycolysis . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| It was found that while relative amount of PFKL mRNA in adult brain was one fourth of that detected in fetal brain the level of PFKM mRNA in adult brain was slightly higher than in fetal tissue , suggesting that PFK expression might be controlled at the transcriptional level . . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| Using somatic cell hybrids and subunit specific antibodies , the PFKM , PFKP , and PFKL loci have been assigned to chromosomes 1 ( region cen leads to q 32 ) , 10p and 21 , respectively . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| The deduced amino acid sequence is highly homologous to PFK M ( muscle ) and PFK L ( liver ) , 69 and 65 % amino acid identity , respectively , especially at substrate binding and catalytic sites , while the allosteric binding sites are less conserved . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| PFK has three subunits : PFK L , PFK M , and PFK P . ^^^ |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P08237 and P17858 |
Pubmed |
SVM Score :0.0 |
| NA |
|