| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Putative cis regulatory elements were identified by comparing promoter regions from three genes that are highly upregulated in chondroinduced hDFs ( BIGH 3 , COL1A2 , and FN 1 ) [ Zhou , S . , Glowacki , J . , Yates , K . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Using constructs ( COL1A2 / CAT ) containing the promoter for the alpha 2 ( 1 ) collagen gene in transient transfection assays with matched pairs of scleroderma and normal skin fibroblasts , we observed higher transcriptional activity of the COL1A2 gene in scleroderma fibroblasts and , in contrast to normal fibroblasts , no further expression was observed in the presence of TGF beta 1 . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Neointima formation and TGF beta 1 immunoreactivity were correlated with increases in fibronectin , collagen alpha 2 ( 1 ) , and collagen alpha 1 ( 3 ) gene expression . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Despite the greatly enhanced rate of secretion of activated TGF beta , metastatic cells exhibited markedly altered responses of TGF beta 1 and TGF beta 2 , being unable to either increase collagen secretion or enhance collagen alpha 2 ( 1 ) or TGF beta 1 mRNA levels . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| We demonstrated that 24 hours of incubation of human dermal fibroblasts with TGF beta 1 ( 1 ng / ml ) under serum free conditions resulted in an elevated expression of TGF beta 1 , collagen alpha 2 ( 1 ) and fibronectin mRNAs that persisted at least 96 hours after removal of TGF beta 1 . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| We have previously identified a strong TGF beta 1 responsive element ( TbRE ) in the upstream promoter sequence of the alpha 2 ( 1 ) collagen ( COL1A2 ) gene . ^^^ Our experiments have shown that TGF beta 1 stimulates COL1A2 transcription by increasing binding of an Sp 1 containing complex ( TbRC ) to the TbRE . ^^^ Here , we provide evidence showing that tyrosine dephosphorylation of nuclear proteins mimics the stimulation of COL1A2 transcription by the TGF beta 1 activated signaling pathway . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that TGF beta 1 and beta 3 have similar but not identical regulatory mechanisms of COL1A2 expression , and that their pathophysiological roles in wound healing might be different from each other . . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Uptake of ( 14 ) C spermidine from the medium by MMC and the transcriptional activity of a segment of the human collagen pro alpha 2 type 1 chain ( COL1A2 ) promoter fused to a luciferase reporter gene were used as indices of TGF beta 1 . ^^^ Transfection of Smad 7 expression vector into MMC abrogated both TGF beta 1 dependent stimulation of spermidine uptake and COL1A2 promoter activity . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| This study compared the association of OSF and polymorphisms of six collagen related genes , collagen 1A1 and 1A2 ( COL1A1 and COL1A2 ) , collagenase 1 ( COLase ) , transforming growth factor beta 1 ( TGF beta 1 ) , lysyl oxidase ( LYOXase ) , and cystatin C ( CST 3 ) , between patients with low and high exposure to betel quids . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| We previously showed that Smad 3 promotes COL1A2 gene activation by TGF beta 1 in human mesangial cells . ^^^ Inhibition of PKCdelta by rottlerin decreased basal and TGF beta 1 stimulated collagen 1 production , mRNA expression , and COL1A2 promoter activity , whereas blockade of conventional PKCs by G 6976 had little or no effect . ^^^ TGF beta 1 stimulated PKCdelta activity positively regulates Smad transcriptional activity and is required for COL1A2 gene transcription . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Transforming growth factor beta 1 ( TGF beta 1 ) induces alpha 2 ( 1 ) collagen gene ( COL1A2 ) expression in mesangial cells through physical and functional cooperation of Smad proteins and Sp 1 . ^^^ RESULTS : TGF beta 1 induced COL1A2 gene expression in cultured mouse mesangial cells which was blocked by overexpression of inhibitory Smad 7 . ^^^ TGF beta 1 induced interaction of endogenous p 300 with Smad2 / 3 , and a dominant negative construct of p 300 inhibited the TGF beta 1 induced COL1A2 expression in cultured mouse mesangial cells . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| For fetal dominating genes , 28 clones matched to 10 known genes ( COL1A2 , COL3A1 , endomucin , HBG 1 , HBG 2 , PCBP 2 , LOC 51144 , TGFBI , vinculin and PND ) , 9 clones to 5 cDNAs of unknown functions ( accession AK 021715 , AF 085867 , AB 040948 , AB 051460 and AB 051512 ) and 2 clones had homology to hEST sequences . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| We previously showed that Smad 3 promotes COL1A2 gene activation by TGF beta 1 in human glomerular mesangial cells . ^^^ Similarly , LY 294002 or an Akt dominant negative construct blocks TGF beta 1 induction of COL1A2 promoter activity . ^^^ Thus , although the PI3K PDK 1 Akt pathway alone is insufficient to stimulate COL1A2 gene transcription , its activation by TGF beta 1 enhances Smad 3 transcriptional activity leading to increased collagen 1 expression in human mesangial cells . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Colonic expression of collagens ( Col1a2 , Col3a2 ) , ECM remodeling genes ( matrix metalloproteinase [ MMP ] 1 , 3 , and tissue inhibitor of matrix metalloproteinase [ TIMP ] 1 ) , and inflammation modulating cytokines ( tumor necrosis factor alpha [ TNF alpha ] , interferon gamma [ IFN gamma ] , transforming growth factor beta 1 [ TGF beta 1 ] , and insulin like growth factor 1 [ IGF 1 ] ) were assessed by semiquantitative reverse transcription polymerase chain reaction . ^^^ RESULTS : Colons of TNBS treated mice contained acute and chronic inflammatory infiltrates , increased collagen , fibrogenic tissue architecture , and increased expression of TNF alpha , TGF beta 1 , IGF 1 , Col1a2 , MMP 1 , and TIMP 1 . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| These include significant time course differences in the expression of COL1A2 and TGF beta 1 mRNAs and inability of neutralizing antibodies to TGF beta 1 to inhibit acetaldehyde dependent collagen gene transcription and Smad 3 phosphorylation . ^^^ In conclusion , acetaldehyde dependent mechanisms involved in COL1A2 upregulation are similar , but not identical , to those of TGF beta 1 . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| COL1A2 promoter activity was dose dependently induced by TGF beta 1 , which was further augmented by adenoviral overexpression of Smad 3 , but was downregulated by Smad 7 . ^^^ Under the identical condition , adenoviral overexpression of BMP 7 attenuated the TGF beta 1 dependent COL1A2 promoter activity . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| Northern blot studies revealed a progressive increase in collagen alpha 2 ( 1 ) , TGF beta 1 and endoglin mRNA expression in L kidneys when compared with the corresponding non ligated ( NL ) kidneys from the animals subjected to left UUO . ^^^ Seventeen days after UUO , significant increases in collagen alpha 2 ( 1 ) , collagen alpha 1 ( 4 ) , TGF beta 1 and endoglin mRNA levels were detected in L kidneys vs NL kidneys . ^^^ |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q15582 and P08123 |
Pubmed |
SVM Score :0.0 |
| NA |
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