Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.52003185
The XPF protein forms a tight complex with ERCC 1 and this complex functions as a structure specific endonuclease responsible for the 5 ' incision during DNA excision repair . 0.52003185^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.6829555
To characterize the specific interaction between XPF and ERCC 1 , we expressed the human ERCC 1 binding domain of XPF ( XPF EB ) and the XPF binding domain of ERCC 1 ( ERCC 1 FB ) in Escherichia coli . 0.6829555^^^ Cross linking experiments at high salt concentrations revealed that XPF interacts with ERCC 1 mainly through hydrophobic interactions . 0.67310805^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Partial characterization of the DNA repair protein complex , containing the ERCC 1 , ERCC 4 , ERCC 11 and XPF correcting activities . ^^^ The nucleotide excision repair ( NER ) protein ERCC 1 is part of a functional complex , which harbors in addition the repair correcting activities of ERCC 4 , ERCC 11 and human XPF . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
ERCC 1 and XPF correcting activity copurified with an ERCC 1 binding polypeptide of 110 kDa that was absent in XP F cell extract . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Formation of a ternary complex by human XPA , ERCC 1 , and ERCC 4 ( XPF ) excision repair proteins . ^^^ The XPA bound fraction complemented cell free extracts of excision repair cross complementing 1 ( ERCC 1 ) , ERCC 4 ( XP F ) , and XP A mutants . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Co correction of the ERCC 1 , ERCC 4 and xeroderma pigmentosum group F DNA repair defects in vitro . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Evidence for a repair enzyme complex involving ERCC 1 and complementing activities of ERCC 4 , ERCC 11 and xeroderma pigmentosum group F . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Although significant homology exists between Rad 10 and the human DNA repair protein Ercc 1 in this region , we were unable to detect any interaction between Ercc 1 and Rad 1 proteins . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We confirmed that ERCC 1 and ERCC 4 play a role in the repair of cis diamminedichloroplatinum ( DDP ) and Melphalan ( L PAM ) induced DNA damage , while a marginal role of ERCC 2 and ERCC 3 in the cellular response to DDP and L PAM treatment has been observed . ^^^ ERCC 1 , ERCC 2 , ERCC 3 and ERCC 4 , but not ERCC 6 gene products seem to be involved in removing the lesions caused by Tallimustine and CC 1065 , minor groove alkylating agents that alkylate N 3 adenine in a sequence specific manner . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
On the other hand , we observed a very faint PCNA staining in XP2YO ( XP F ) cells , expressing no detectable ERCC 1 or XPF protein , after UV irradiation . 10 rays induced PCNA staining in all cell lines with a similar staining pattern , and radiosensitivity was exactly the same between XL 216 7 and XLgfPAneo 1 cells . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We compared the sensitivity of the wild type CHO cell line , AA 8 , with that of the CHO mutant cell lines UV 4 and UV 20 ( ERCC 1 ) , UV 5 ( ERCC 2 ) , UV 24 ( ERCC 3 ) , UV 41 ( ERCC 4 ) , UV 135 ( ERCC 5 ) , UV 61 ( ERCC 6 ) , and EM 9 ( XRCC 1 ) . ^^^ Thus mutations in the ERCC 1 and ERCC 4 genes impart a hypersensitivity to CP analogs . ^^^ CONCLUSIONS : Our findings confirm the general pattern indicating that the ERCC 1 and ERCC 4 gene products are crucial for the repair of 4HC induced DNA damage , while the other nucleotide excision repair genes examined are relatively unimportant . ^^^ These data suggest that the hypersensitivity of ERCC 1 and ERCC 4 mutants to DNA crosslinking agents may reflect a defect in recombinational repair rather than nucleotide excision repair . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The XPF protein was purified from mammalian cells in a tight complex with ERCC 1 . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The human DNA repair protein ERCC 1 resides in a complex together with the ERCC 4 , ERCC 11 and XP F correcting activities , thought to perform the 5 ' strand incision during nucleotide excision repair ( NER ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Among the NER genes , ERCC 4 and ERCC 1 are also uniquely involved in removing DNA interstrand cross linking damage . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Reconstitution of human excision nuclease with recombinant XPF ERCC 1 complex . ^^^ The human XPF ERCC 1 protein complex is one of several factors known to be required for general nucleotide excision repair . ^^^ To determine whether XPF ERCC 1 completes a set of six proteins that are sufficient to carry out excision repair , the human XPF and ERCC 1 cDNAs were coexpressed in Sf 21 insect cells from a baculovirus vector . ^^^ Furthermore , reconstitution of the human excision nuclease was observed with a mixture of five repair factors ( XPA , XPC , XPG , TFIIH , and RPA ) and the recombinant XPF ERCC 1 , thus verifying that no additional protein factors are needed for the specific dual incisions characteristic of human excision repair . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The ERCC 1 protein has been predicted to form part of a tight complex with a protein partner , the yet unidentified XPF / ERCC4 protein , in normal human cells . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The extract selectively fails to complement extracts of group 1 and 4 mutants consistent with the notion that the affected proteins , ERCC 1 and ERCC 4 , are part of the same complex and that mutations in one subunit also affect the other component . ^^^ These unusual group 1 and 4 mutants demonstrate that the unique UV and MMC sensitivity phenotypes displayed by these groups can be separated and support the idea that they are the result of distinct repair functions of the corresponding ERCC 1 and ERCC 4 genes : nucleotide excision repair for UV lesions and a separate repair pathway for removal of interstrand crosslinks . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
BACKGROUND : The structure specific ERCC1 / XPF endonuclease complex that contains the ERCC 1 and XPF subunits is implicated in the repair of two distinct types of lesions in DNA : nucleotide excision repair ( NER ) for ultraviolet induced lesions and bulky chemical adducts ; and recombination repair of the very genotoxic interstrand cross links . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
In mammalian cells XPG cleaves 3 ' of the DNA lesion while the ERCC 1 XPF complex makes the 5 ' incision . ^^^ Using the two hybrid system of Saccharomyces cerevisiae we show that only the truncated human ERCC 1 protein is able to interact with the S . pombe Rad 16 protein , which is the fission yeast homologue of human XPF . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The panel of hamster cell mutants that we characterized for their relative sensitivity to fast neutrons and gamma rays includes cell lines with defects in the nucleotide excision repair pathway ; these can be further subdivided into mutants which are defective in nucleotide excision repair alone [ UV 5 ( ERCC 2 ) , UV 24 ( ERCC 3 ) , UV 135 ( ERCC 5 ) and UV 61 ( ERCC 6 ) ] compared to those which have an associated defect in the distinct but overlapping pathway for the repair of DNA crosslinks [ UV 20 ( ERCC 1 ) and UV 41 ( ERCC 4 ) ] . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The C terminal domain of UvrC is homologous with the C terminal part of the ERCC 1 protein which , in a complex with XPF , is responsible for the 5 ' incision reaction in human nucleotide excision repair . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We have evaluated host cell reactivation and cell survival of wild type Chinese hamster ovary cells and of mutants in the NER genes ERCC 1 , ERCC 2 , and ERCC 4 after treatment with the methylating compounds dimethylsulfate and methylnitrosourea . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
In mammalian nucleotide excision repair ( NER ) , the ERCC 1 protein is known to act as a complex with ERCC 4 ( XPF ) protein , which is necessary for stability of ERCC 1 , and this complex introduces an incision on the 5 ' side of a damaged site in DNA . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Mapping of interaction domains between human repair proteins ERCC 1 and XPF . ^^^ ERCC 1 XPF is a heterodimeric protein complexinvolved in nucleotide excision repair and recombinational processes . ^^^ In repair , ERCC 1 XPF and Rad 10 Rad1 make an incision on the the 5 ' side of the lesion . ^^^ All XP F patients examined demonstrate reduced levels of XPF and ERCC 1 protein , suggesting that proper complex formation is required for stability of the two proteins . ^^^ To better understand the molecular and clinical consequences of mutations in the ERCC 1 XPF complex , we decided to map the interaction domains between the two subunits . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Sensitivity of group F xeroderma pigmentosum cells to UV and mitomycin C relative to levels of XPF and ERCC 1 overexpression . ^^^ The XPF and ERCC 1 proteins form a tight complex and function as an endonuclease to incise on the 5 ' side of pyrimidine dimers in DNA . ^^^ We transfected XP F cells with the plasmids expressing XPF or ERCC 1 and examined levels of both proteins in the cells . ^^^ Although XP F cells are sensitive to UV and mitomycin C ( MMC ) , cells overexpressing XPF expressed ERCC 1 as well and resistance to UV and MMC was restored to the normal level . ^^^ In contrast , cells overexpressing ERCC 1 did not express XPF and were still sensitive to UV and MMC . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
In this study , the mRNA levels of the different genes involved in NER ( ERCC 1 , XPA , XPB , XPC , XPD , XPF ) were examined in a panel of eight different human cancer cell lines , together with the overall DNA repair capacity using a host cell reactivation assay of a damaged plasmid . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
ERCC 4 mRNA and poly ( ADP ribose ) polymerase levels were similar in both cell lines , and ERCC 1 mRNA levels were 2 to 2 . 5 fold lower in D 283 Med ( 4 HCR ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The XP FR 2 cells expressed high level of XPF protein as well as ERCC 1 protein , although their parental XP F cells expressed extremely low level of both proteins . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Nine different amino acid substitution variants have been identified in resequencing of the exons of three nucleotide excision repair genes ( ERCC 1 , XPD , and XPF ) , a gene involved in double strand break repair / recombination genes ( XRCC 3 ) , and a gene functioning in base excision repair and the repair of radiation induced damage ( XRCCI ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Domain mapping of the DNA binding , endonuclease , and ERCC 1 binding properties of the human DNA repair protein XPF . ^^^ To characterize the biochemical function of XPF , we have expressed and purified the independent 104 kDa recombinant XPF protein from E . coli and determined that it is an endonuclease and can bind DNA in the absence of the ERCC 1 subunit . ^^^ To investigate the significance of the two predicted domains , a series of XPF deletion fragments spanning the entire protein were designed and examined for DNA binding , endonuclease activity , and ERCC 1 subunit binding . ^^^ Our results indicate that the N terminal 378 amino acids of XPF are capable of binding and hydrolyzing DNA , while the C terminal 214 residues are capable of binding specifically to ERCC 1 . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Moreover , increased levels of Xpf and Ercc 1 mRNAs correlated with meiotic and early postmeiotic spermatogenic cells . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The 5 ' incision is made by a heterodimer of XPF and ERCC 1 ( Rad1p Rad10p in Saccharomyces cerevisiae ) , and the 3 ' incision is made by XPG ( Rad2p in S . cerevisiae ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Such genes include ERCC 1 , XPA , XPB , XPD , XPF , and XPG . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
XPF and ERCC 1 mutants ( defective in genes required for NER and some types of recombination ) and XRCC 2 and XRCC 3 mutants ( defective in RAD 51 related homologous recombination genes ) were highly sensitive to HN 2 . ^^^ In agreement with their extreme sensitivity , the XPF and ERCC 1 mutants were defective in the incision or `` unhooking ' ' step of ICL repair . ^^^ DSB induction and repair were normal in all the NER mutants , including XPF and ERCC 1 . ^^^ These results define a role for XPF and ERCC 1 in the excision of ICLs , but not in the recombinational components of cross link repair . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We have used the same study persons to investigate the correlation between expression of eight genes involved in nucleotide excision repair and DNA repair capacity . mRNA levels of XPA , XPB , XPC , XPD , XPF , XPG , CSB and ERCC 1 in primary lymphocytes from 33 individuals were quantified by dot blots and normalized to beta actin . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Large heterology repair in nuclear extracts of human cells is also independent of the XPF gene product , and extracts of Chinese hamster ovary cells deficient in the ERCC 1 and ERCC 4 gene products also support the reaction . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The resultant NER complex is a cooperative assembly of XPF , ERCC 1 , XPA , RPA , and XPG subunits . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
DNA synthesis is compromised in extracts from mutants ( deficient in ERCC 1 , XPF , XRCC 2 , and XRCC 3 ) which are all sensitive to DNA cross linking agents but is normal in extracts from mutants ( XP A , XP C , and XP G ) which are much less sensitive . ^^^ The DNA synthesis deficit in ERCC 1 and XPF deficient extracts is restored by addition of purified ERCC 1 XPF heterodimer . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
XPF forms a heterodimeric complex with ERCC 1 and is required for the repair of DNA interstrand cross links . ^^^ These mAbs were examined for their ability to differentially inhibit production of dual incisions in DNA by normal human chromatin associated protein extracts that contain XPF and ERCC 1 . ^^^ Since this C terminal region corresponds to the proposed site of interaction of ERCC 1 with XPF , these results suggest that binding of ERCC 1 to XPF is critical for its ability to produce the 5 ' and 3 ' incisions at the site of an interstrand cross link , possibly through activation or regulation of the endonucleolytic activity of the N terminal domain of XPF . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The processing of psoralen ICLs is also dependent upon the nucleotide excision repair proteins Ercc 1 and Xpf but not upon other components of the excision stage of this pathway or upon Fanconi anemia proteins . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Recently , a model of ICL repair has been proposed , in which mammalian cells use novel excision repair reactions ( requiring the XPF and ERCC 1 proteins ) to uncouple the crosslink . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Some of these cell lines , specifically certain rodent mutants with severe defects in the ERCC 1 and XPF genes , are dramatically sensitive to crosslinking agents such as phosphoramide mustard ( PM ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We have also shown using hamster mutants that both ERCC 1 and XPF are required for the observed incorporation . ^^^ These findings support the hypothesis that the activities of XPF in nucleotide excision repair ( NER ) and crosslink repair are separable , and that mutations in XPF patients result in the abolition of NER , but not recombinational repair pathways , which are likely to be essential as has been observed in ERCC 1 homozygous / mice . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Activity of individual ERCC 1 and XPF subunits in DNA nucleotide excision repair . ^^^ ERCC 1 XPF is a structure specific nuclease with two subunits , ERCC 1 and XPF . ^^^ We produced recombinant ERCC 1 and XPF separately in Escherichia coli as soluble proteins . ^^^ XPF and ERCC 1 are unstable in mammalian cells in the absence of their partners but we found , surprisingly , that ERCC 1 alone could confer some repair to extracts from ERCC 1 defective cells . ^^^ This indicated that a small amount of active XPF was present in ERCC 1 extracts , and immunoassays showed this to be the case . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We have made a replication defective adenovirus , AdCMV FlagXPA ( 59 114 ) , that expresses a truncated form of XPA encompassing amino acids 59 114 sufficient for binding to the excision repair cross complementing protein 1 ( ERCC 1 ) / xeroderma pigmentosum complementation group F ( XPF ) nuclease essential for making an incision 5 ' of the damage . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
RESULTS : Mammalian cell lines ( XPB , XPD , XPF , XPG , and ERCC 1 ) deficient in the nucleotide excision repair ( NER ) gene products show resistance to Et 743 . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Toenail arsenic levels were inversely correlated with expression of critical members of the nucleotide excision repair complex , ERCC 1 ( r ( 2 ) = 0 . 82 , p < 0 . 0001 ) , XPF ( r ( 2 ) = 0 . 56 , p < 0 . 002 ) , and XPB ( r ( 2 ) = 0 . 75 , p < 0 . 0001 ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
MUS 81 has sequence homology to another DNA nuclease , XPF , which , with its partner ERCC 1 , makes the 5 ' incision during nucleotide excision repair . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We have compared the frequency of polymorphisms in the NER genes , XPD , XPF , XPG , ERCC 1 ; in the BER gene , XRCC 1 ; and in the RR gene , XRCC 3 ; in colorectal cancer patients and in a control group . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Mutations in ERCC 1 , XPA , XPB , XPC , XPF , XPG and CSB dramatically reduced TER . ^^^ Mutations in ERCC 1 , XPC , XPF , XPG and CSB suppressed transfection so that the TER was significantly below 1 . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Defects in interstrand cross link uncoupling do not account for the extreme sensitivity of ERCC 1 and XPF cells to cisplatin . ^^^ In contrast to cells bearing mutations in other components of the nucleotide excision repair apparatus ( XPB , XPD , XPG and CSB ) , cells defective for the ERCC 1 XPF structure specific nuclease are highly sensitive to cisplatin . ^^^ To determine if the extreme sensitivity of XPF and ERCC 1 cells to cisplatin results from specific defects in the repair of either intrastrand or interstrand cross links we measured the elimination of both lesions in a range of nucleotide excision repair Chinese hamster mutant cell lines , including XPF and ERCC 1 defective cells . ^^^ These results demonstrate that the high cisplatin sensitivity of ERCC 1 and XPF cells likely results from a defect other than in excision repair . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Ercc 1 is essential for nucleotide excision repair ( NER ) but , unlike other NER proteins , Ercc 1 and Xpf are also involved in recombination repair pathways . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Thirty studies of polymorphisms in OGG 1 , XRCC 1 , ERCC 1 , XPC , XPD , XPF , BRCA 2 , and XRCC 3 were identified in the April 30 , 2002 MEDLINE database ( National Center for Biotechnology Information . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The formation of UV induced chromosome aberrations involves ERCC 1 and XPF but not other nucleotide excision repair genes . ^^^ ERCC 1 XPF , through its role in nucleotide excision repair ( NER ) , is essential for the repair of DNA damage caused by UV light . ^^^ ERCC 1 XPF is also involved in recombinational repair processes distinct from NER . ^^^ This implies that ERCC 1 XPF , but not other NER proteins , is involved in the formation of UV induced chromosome aberrations , presumably through the role of ERCC 1 XPF in recombinational repair pathways rather than NER . ^^^ We suggest that ERCC 1 XPF may be involved in the bypass / repair of DNA damage in replicating DNA by an exchange mechanism involving single strand annealing between non homologous chromosomes . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The structure specific endonuclease Ercc 1 Xpf is required to resolve DNA interstrand cross link induced double strand breaks . ^^^ However , genetic data implicate the Ercc 1 Xpf endonuclease and proteins required for homologous recombination mediated double strand break ( DSB ) repair . ^^^ To examine the role of Ercc 1 Xpf in ICL repair , we monitored the phosphorylation of histone variant H2AX ( gamma H2AX ) . ^^^ MMC triggered sister chromatid exchanges in wild type cells but chromatid fusions in Ercc 1 ( / ) and Xpf mutant cells , indicating that in their absence , repair of DSBs is prevented . ^^^ Collectively , these data support a role for Ercc 1 Xpf in processing ICL induced DSBs so that these cytotoxic intermediates can be repaired by homologous recombination . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The xeroderma pigmentosum complementation group F ( XPF ) protein is a structure specific endonuclease in a complex with ERCC 1 and is essential for nucleotide excision repair ( NER ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The model rationalises published biochemical data and implies a novel role for the ERCC 1 subunit of eukaryotic XPF complexes . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The expressions of the 5 FU related genes TS , DPD , and TP and of the cisplatin related genes ERCC 1 , ERCC 4 , KU 80 , and GADD45A were analyzed by quantitative real time PCR . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Selected SNPs belong to the following genes : ADH1B , ALDH 2 , APEX , CDKN2A , COMT , CYP1A1 , CYP1A2 , CYP1B1 , CYP2A6 , CYP2C19 , CYP2C9 , CYP2E1 , CYP3A4 , DRD 2 , DRD 4 , EPHX 1 , ERCC 1 , ERCC 2 , ERCC 4 , ERCC 5 , GRPR , GSTA 4 , GSTM 3 , GSTP 1 , GSTT 2 , LIG 3 , MDM 2 , MGMT , MPO , NAT 1 , NAT 2 , NQO 1 , OGG 1 , PCNA , POLB , SLC6A3 , SOD 2 , TP 53 , XRCC 1 , XRCC 2 , XRCC 3 , and XRCC 9 . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
XPF , in association with ERCC 1 , constitutes a structure specific endonuclease that makes an incision 5 ' to the photodamage . ^^^ XPF ERCC 1 has also been implicated in both removal of interstrand DNA cross links and homology mediated recombination and in immunoglobulin class switch recombination ( CSR ) . ^^^ The complex phenotype suggests that XPF ERCC 1 is involved in multiple DNA repair processes . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Reduced levels of XPA , ERCC 1 and XPF DNA repair proteins in testis tumor cell lines . ^^^ We previously investigated 2 testis tumor cell lines with a low capacity to remove cisplatin induced DNA damage and found that they had low levels of the DNA nucleotide excision repair proteins XPA , ERCC 1 and XPF . ^^^ To determine whether low levels of XPA , ERCC 1 and XPF proteins are characteristic of testis tumor cell lines , we investigated 35 cell lines derived from cancers to determine whether groups of cell lines from diverse tissue origins differ from one another in constitutive levels of these NER proteins . ^^^ Only the 6 testis tumor cell lines showed significantly lower mean levels of XPA ( p = 0 . 001 ) , XPF ( p = 0 . 001 ) and ERCC 1 ( p = 0 . 004 ) proteins from the other groups . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Three NER proteins : ERCC 1 , XPF , and XPA , are present at low levels in testis tumor cell lines , and addition of these proteins to protein extracts of testis tumor cells increases their in vitro DNA repair capacity to normal levels . ^^^ The levels of the mRNA transcripts for ERCC 1 , XPF , and XPA were measured in a panel of 14 different human cancer cell lines , using real time PCR . ^^^ The significantly lower levels of ERCC 1 , XPF , and XPA protein in testis tumor cell lines can not be explained solely by differences in transcriptional efficiency or mRNA stability . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Genes involved in DNA repair , such as XRCC 5 , ERCC 5 , ERCC 1 , RAD9A , ERCC 4 and the gene encoding DNA PK , were found to be increased to a higher level in A 549 cells than in NCI H 446 cells . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Crystal structure and DNA binding functions of ERCC 1 , a subunit of the DNA structure specific endonuclease XPF ERCC 1 . ^^^ Human XPF ERCC 1 is a DNA endonuclease that incises a damaged DNA strand on the 5 ' side of a lesion during nucleotide excision repair and has additional role ( s ) in homologous recombination and DNA interstrand crosslink repair . ^^^ We show that a truncated form of XPF lacking the N terminal helicase like domain in complex with ERCC 1 exhibits a structure specific endonuclease activity with similar specificity to that of full length XPF ERCC 1 . ^^^ The XPF ERCC 1 HhH2 domain heterodimer contains two independent ssDNA binding surfaces , which are revealed by a crystal structure of the protein complex . ^^^ A crystal structure of the central domain of ERCC 1 shows its fold is strikingly similar to that of the nuclease domains of the archaeal Mus81 / XPF homologs , despite very low sequence homology . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
RNA expression levels of 5 FU metabolism associated genes thymidylate synthase , thymidine phosphorylase , dihydropyrimidine dehydrogenase , methylenetetrahydrofolate reductase , MAP 7 , and ELF 3 , of platinum and taxane related genes caldesmon , ERCC 1 , ERCC 4 , HER 2 / neu , and GADD 45 , and of multidrug resistance gene MRP 1 were determined using real time reverse transcriptase PCR . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Genotyping assays were done for ERCC 1 , ERCC 2 ( XPD ) , ERCC 4 ( XPF ) , ERCC 5 ( XPG ) , XPA , and XPC . ^^^ No appreciable differences between cases and controls were observed in the genotype distributions of ERCC 1 ( c8092a and c19007t ) , ERCC 2 ( D312N , K751Q , and c22541a ) , ERCC 4 ( R415Q and t30028c ) , or ERCC 5 ( D1104H ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We analyzed 13 polymorphisms in seven DNA repair genes belonging to different repair pathways [ 10 ray repair cross complementing group 1 ( XRCC 1 ) : 26304C > T , 26651A > G , 28152A > G ; xeroderma pigmentosum D ( XPD ) : 23591A > G , 35931A > C ; excision repair complementing defective in Chinese hamster , group 1 ( ERCC 1 ) : 19007C > T ; XRCC 3 : 4541T > C , 17893A > G , 18067C > T ; proliferating cell nuclear antigen ( PCNA ) : 6084G > C ; ERCC 4 : 30028C > T , 30147A > G ; and XRCC 2 31479A > G ] in 317 incident bladder cancer patients and 317 controls . ^^^ XRCC 3 , XRCC 1 , ERCC 4 , and XPD ERCC 1 haplotype frequencies were estimated by the maximum likelihood method . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The ERCC 1 domain folds properly only in the presence of the XPF domain , which implies a role for XPF as a scaffold for the folding of ERCC 1 . ^^^ NMR titration data show that only the ERCC 1 domain of the ERCC1 / XPF complex is involved in DNA binding . ^^^ On the basis of these findings , we propose a model for the targeting of XPF nuclease via ERCC 1 mediated interactions in the context of nucleotide excision repair . . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
Lower OERs have also been reported in cells deficient in XPF and ERCC 1 , which , similar to homologous recombination genes , are known to play a role in cross link repair . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The risk of OSCC was evaluated in 10 genetic polymorphisms of nine genes , such as CYP1A1 , CYP2E1 , GSTM 1 , GSTT 1 , XPA , XPC , XPC , XPF and ERCC 1 . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
We evaluated the influence of common genetic variation in the NER pathway on bladder cancer risk by analyzing 22 single nucleotide polymorphisms ( SNP ) in seven NER genes ( XPC , RAD23B , ERCC 1 , ERCC 2 , ERCC 4 , ERCC 5 , and ERCC 6 ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
ERCC 1 is an excision nuclease within the nucleotide excision repair pathway that forms a heterodimer with XPF . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
The candidate genes belong to different DNA repair pathways : base excision repair ( OGG 1 , LIG 3 , APEX , POLB , XRCC 1 , PCNA , and MUTYH ) , nucleotide excision repair ( ERCC 1 , ERCC 2 , ERCC 4 , and ERCC 5 ) , double strand breaks repair ( XRCC 2 , XRCC 3 , and XRCC 9 ) , and reversion repair ( MGMT ) genes . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
XP proteins are key players in several steps of the NER process , including DNA strand discrimination ( XPA , in complex with replication protein A ) , repair complex formation ( XPC , in complex with hHR23B ; XPF , in complex with ERCC 1 ) and repair factor recruitment ( transcription factor IIH , in complex with XPG ) . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
RNA expression levels of 5 FU metabolism associated genes thymidylate synthase ( TS ) , thymidine phosphorylase ( TP ) , dihydropyrimidine dehydrogenase ( DPD ) , methylenetetrahydrofolate reductase ( MTHFR ) , MAP 7 , ELF 3 , as well as of platinum and taxane associated related genes caldesmon , excision cross complementing genes ( ERCC 1 and ERCC 4 ) HER 2 neu , DNA damage inducible gene 45 ( GADD 45 ) and multidrug resistance genes ( MDR 1 , MRP 1 ) were determined using real time RT PCR . ^^^
Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
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Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
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Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
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Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
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Interacting proteins: P07992 and Q92889	Pubmed	SVM Score :0.0
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Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.52003185
The XPF protein forms a tight complex with ERCC 1 and this complex functions as a structure specific endonuclease responsible for the 5 ' incision during DNA excision repair . 0.52003185^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.6829555
To characterize the specific interaction between XPF and ERCC 1 , we expressed the human ERCC 1 binding domain of XPF ( XPF EB ) and the XPF binding domain of ERCC 1 ( ERCC 1 FB ) in Escherichia coli . 0.6829555^^^ Cross linking experiments at high salt concentrations revealed that XPF interacts with ERCC 1 mainly through hydrophobic interactions . 0.67310805^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Partial characterization of the DNA repair protein complex , containing the ERCC 1 , ERCC 4 , ERCC 11 and XPF correcting activities . ^^^ The nucleotide excision repair ( NER ) protein ERCC 1 is part of a functional complex , which harbors in addition the repair correcting activities of ERCC 4 , ERCC 11 and human XPF . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
ERCC 1 and XPF correcting activity copurified with an ERCC 1 binding polypeptide of 110 kDa that was absent in XP F cell extract . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Formation of a ternary complex by human XPA , ERCC 1 , and ERCC 4 ( XPF ) excision repair proteins . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
On the other hand , we observed a very faint PCNA staining in XP2YO ( XP F ) cells , expressing no detectable ERCC 1 or XPF protein , after UV irradiation . 10 rays induced PCNA staining in all cell lines with a similar staining pattern , and radiosensitivity was exactly the same between XL 216 7 and XLgfPAneo 1 cells . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The XPF protein was purified from mammalian cells in a tight complex with ERCC 1 . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
BACKGROUND : The structure specific ERCC1 / XPF endonuclease complex that contains the ERCC 1 and XPF subunits is implicated in the repair of two distinct types of lesions in DNA : nucleotide excision repair ( NER ) for ultraviolet induced lesions and bulky chemical adducts ; and recombination repair of the very genotoxic interstrand cross links . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
In mammalian cells XPG cleaves 3 ' of the DNA lesion while the ERCC 1 XPF complex makes the 5 ' incision . ^^^ Using the two hybrid system of Saccharomyces cerevisiae we show that only the truncated human ERCC 1 protein is able to interact with the S . pombe Rad 16 protein , which is the fission yeast homologue of human XPF . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The C terminal domain of UvrC is homologous with the C terminal part of the ERCC 1 protein which , in a complex with XPF , is responsible for the 5 ' incision reaction in human nucleotide excision repair . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The XP FR 2 cells expressed high level of XPF protein as well as ERCC 1 protein , although their parental XP F cells expressed extremely low level of both proteins . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Nine different amino acid substitution variants have been identified in resequencing of the exons of three nucleotide excision repair genes ( ERCC 1 , XPD , and XPF ) , a gene involved in double strand break repair / recombination genes ( XRCC 3 ) , and a gene functioning in base excision repair and the repair of radiation induced damage ( XRCCI ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
In mammalian nucleotide excision repair ( NER ) , the ERCC 1 protein is known to act as a complex with ERCC 4 ( XPF ) protein , which is necessary for stability of ERCC 1 , and this complex introduces an incision on the 5 ' side of a damaged site in DNA . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Reconstitution of human excision nuclease with recombinant XPF ERCC 1 complex . ^^^ The human XPF ERCC 1 protein complex is one of several factors known to be required for general nucleotide excision repair . ^^^ To determine whether XPF ERCC 1 completes a set of six proteins that are sufficient to carry out excision repair , the human XPF and ERCC 1 cDNAs were coexpressed in Sf 21 insect cells from a baculovirus vector . ^^^ Furthermore , reconstitution of the human excision nuclease was observed with a mixture of five repair factors ( XPA , XPC , XPG , TFIIH , and RPA ) and the recombinant XPF ERCC 1 , thus verifying that no additional protein factors are needed for the specific dual incisions characteristic of human excision repair . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The ERCC 1 protein has been predicted to form part of a tight complex with a protein partner , the yet unidentified XPF / ERCC4 protein , in normal human cells . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
DNA synthesis is compromised in extracts from mutants ( deficient in ERCC 1 , XPF , XRCC 2 , and XRCC 3 ) which are all sensitive to DNA cross linking agents but is normal in extracts from mutants ( XP A , XP C , and XP G ) which are much less sensitive . ^^^ The DNA synthesis deficit in ERCC 1 and XPF deficient extracts is restored by addition of purified ERCC 1 XPF heterodimer . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Mapping of interaction domains between human repair proteins ERCC 1 and XPF . ^^^ ERCC 1 XPF is a heterodimeric protein complexinvolved in nucleotide excision repair and recombinational processes . ^^^ In repair , ERCC 1 XPF and Rad 10 Rad1 make an incision on the the 5 ' side of the lesion . ^^^ All XP F patients examined demonstrate reduced levels of XPF and ERCC 1 protein , suggesting that proper complex formation is required for stability of the two proteins . ^^^ To better understand the molecular and clinical consequences of mutations in the ERCC 1 XPF complex , we decided to map the interaction domains between the two subunits . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Sensitivity of group F xeroderma pigmentosum cells to UV and mitomycin C relative to levels of XPF and ERCC 1 overexpression . ^^^ The XPF and ERCC 1 proteins form a tight complex and function as an endonuclease to incise on the 5 ' side of pyrimidine dimers in DNA . ^^^ We transfected XP F cells with the plasmids expressing XPF or ERCC 1 and examined levels of both proteins in the cells . ^^^ Although XP F cells are sensitive to UV and mitomycin C ( MMC ) , cells overexpressing XPF expressed ERCC 1 as well and resistance to UV and MMC was restored to the normal level . ^^^ In contrast , cells overexpressing ERCC 1 did not express XPF and were still sensitive to UV and MMC . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
In this study , the mRNA levels of the different genes involved in NER ( ERCC 1 , XPA , XPB , XPC , XPD , XPF ) were examined in a panel of eight different human cancer cell lines , together with the overall DNA repair capacity using a host cell reactivation assay of a damaged plasmid . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Large heterology repair in nuclear extracts of human cells is also independent of the XPF gene product , and extracts of Chinese hamster ovary cells deficient in the ERCC 1 and ERCC 4 gene products also support the reaction . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The resultant NER complex is a cooperative assembly of XPF , ERCC 1 , XPA , RPA , and XPG subunits . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Domain mapping of the DNA binding , endonuclease , and ERCC 1 binding properties of the human DNA repair protein XPF . ^^^ To characterize the biochemical function of XPF , we have expressed and purified the independent 104 kDa recombinant XPF protein from E . coli and determined that it is an endonuclease and can bind DNA in the absence of the ERCC 1 subunit . ^^^ To investigate the significance of the two predicted domains , a series of XPF deletion fragments spanning the entire protein were designed and examined for DNA binding , endonuclease activity , and ERCC 1 subunit binding . ^^^ Our results indicate that the N terminal 378 amino acids of XPF are capable of binding and hydrolyzing DNA , while the C terminal 214 residues are capable of binding specifically to ERCC 1 . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Moreover , increased levels of Xpf and Ercc 1 mRNAs correlated with meiotic and early postmeiotic spermatogenic cells . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The 5 ' incision is made by a heterodimer of XPF and ERCC 1 ( Rad1p Rad10p in Saccharomyces cerevisiae ) , and the 3 ' incision is made by XPG ( Rad2p in S . cerevisiae ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Such genes include ERCC 1 , XPA , XPB , XPD , XPF , and XPG . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
XPF and ERCC 1 mutants ( defective in genes required for NER and some types of recombination ) and XRCC 2 and XRCC 3 mutants ( defective in RAD 51 related homologous recombination genes ) were highly sensitive to HN 2 . ^^^ In agreement with their extreme sensitivity , the XPF and ERCC 1 mutants were defective in the incision or `` unhooking ' ' step of ICL repair . ^^^ DSB induction and repair were normal in all the NER mutants , including XPF and ERCC 1 . ^^^ These results define a role for XPF and ERCC 1 in the excision of ICLs , but not in the recombinational components of cross link repair . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We have used the same study persons to investigate the correlation between expression of eight genes involved in nucleotide excision repair and DNA repair capacity . mRNA levels of XPA , XPB , XPC , XPD , XPF , XPG , CSB and ERCC 1 in primary lymphocytes from 33 individuals were quantified by dot blots and normalized to beta actin . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We have also shown using hamster mutants that both ERCC 1 and XPF are required for the observed incorporation . ^^^ These findings support the hypothesis that the activities of XPF in nucleotide excision repair ( NER ) and crosslink repair are separable , and that mutations in XPF patients result in the abolition of NER , but not recombinational repair pathways , which are likely to be essential as has been observed in ERCC 1 homozygous / mice . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Recently , a model of ICL repair has been proposed , in which mammalian cells use novel excision repair reactions ( requiring the XPF and ERCC 1 proteins ) to uncouple the crosslink . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Some of these cell lines , specifically certain rodent mutants with severe defects in the ERCC 1 and XPF genes , are dramatically sensitive to crosslinking agents such as phosphoramide mustard ( PM ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Activity of individual ERCC 1 and XPF subunits in DNA nucleotide excision repair . ^^^ ERCC 1 XPF is a structure specific nuclease with two subunits , ERCC 1 and XPF . ^^^ We produced recombinant ERCC 1 and XPF separately in Escherichia coli as soluble proteins . ^^^ XPF and ERCC 1 are unstable in mammalian cells in the absence of their partners but we found , surprisingly , that ERCC 1 alone could confer some repair to extracts from ERCC 1 defective cells . ^^^ This indicated that a small amount of active XPF was present in ERCC 1 extracts , and immunoassays showed this to be the case . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We have made a replication defective adenovirus , AdCMV FlagXPA ( 59 114 ) , that expresses a truncated form of XPA encompassing amino acids 59 114 sufficient for binding to the excision repair cross complementing protein 1 ( ERCC 1 ) / xeroderma pigmentosum complementation group F ( XPF ) nuclease essential for making an incision 5 ' of the damage . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
RESULTS : Mammalian cell lines ( XPB , XPD , XPF , XPG , and ERCC 1 ) deficient in the nucleotide excision repair ( NER ) gene products show resistance to Et 743 . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
XPF forms a heterodimeric complex with ERCC 1 and is required for the repair of DNA interstrand cross links . ^^^ These mAbs were examined for their ability to differentially inhibit production of dual incisions in DNA by normal human chromatin associated protein extracts that contain XPF and ERCC 1 . ^^^ Since this C terminal region corresponds to the proposed site of interaction of ERCC 1 with XPF , these results suggest that binding of ERCC 1 to XPF is critical for its ability to produce the 5 ' and 3 ' incisions at the site of an interstrand cross link , possibly through activation or regulation of the endonucleolytic activity of the N terminal domain of XPF . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The processing of psoralen ICLs is also dependent upon the nucleotide excision repair proteins Ercc 1 and Xpf but not upon other components of the excision stage of this pathway or upon Fanconi anemia proteins . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Defects in interstrand cross link uncoupling do not account for the extreme sensitivity of ERCC 1 and XPF cells to cisplatin . ^^^ In contrast to cells bearing mutations in other components of the nucleotide excision repair apparatus ( XPB , XPD , XPG and CSB ) , cells defective for the ERCC 1 XPF structure specific nuclease are highly sensitive to cisplatin . ^^^ To determine if the extreme sensitivity of XPF and ERCC 1 cells to cisplatin results from specific defects in the repair of either intrastrand or interstrand cross links we measured the elimination of both lesions in a range of nucleotide excision repair Chinese hamster mutant cell lines , including XPF and ERCC 1 defective cells . ^^^ These results demonstrate that the high cisplatin sensitivity of ERCC 1 and XPF cells likely results from a defect other than in excision repair . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Ercc 1 is essential for nucleotide excision repair ( NER ) but , unlike other NER proteins , Ercc 1 and Xpf are also involved in recombination repair pathways . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Thirty studies of polymorphisms in OGG 1 , XRCC 1 , ERCC 1 , XPC , XPD , XPF , BRCA 2 , and XRCC 3 were identified in the April 30 , 2002 MEDLINE database ( National Center for Biotechnology Information . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The formation of UV induced chromosome aberrations involves ERCC 1 and XPF but not other nucleotide excision repair genes . ^^^ ERCC 1 XPF , through its role in nucleotide excision repair ( NER ) , is essential for the repair of DNA damage caused by UV light . ^^^ ERCC 1 XPF is also involved in recombinational repair processes distinct from NER . ^^^ This implies that ERCC 1 XPF , but not other NER proteins , is involved in the formation of UV induced chromosome aberrations , presumably through the role of ERCC 1 XPF in recombinational repair pathways rather than NER . ^^^ We suggest that ERCC 1 XPF may be involved in the bypass / repair of DNA damage in replicating DNA by an exchange mechanism involving single strand annealing between non homologous chromosomes . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Toenail arsenic levels were inversely correlated with expression of critical members of the nucleotide excision repair complex , ERCC 1 ( r ( 2 ) = 0 . 82 , p < 0 . 0001 ) , XPF ( r ( 2 ) = 0 . 56 , p < 0 . 002 ) , and XPB ( r ( 2 ) = 0 . 75 , p < 0 . 0001 ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
MUS 81 has sequence homology to another DNA nuclease , XPF , which , with its partner ERCC 1 , makes the 5 ' incision during nucleotide excision repair . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We have compared the frequency of polymorphisms in the NER genes , XPD , XPF , XPG , ERCC 1 ; in the BER gene , XRCC 1 ; and in the RR gene , XRCC 3 ; in colorectal cancer patients and in a control group . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Mutations in ERCC 1 , XPA , XPB , XPC , XPF , XPG and CSB dramatically reduced TER . ^^^ Mutations in ERCC 1 , XPC , XPF , XPG and CSB suppressed transfection so that the TER was significantly below 1 . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The structure specific endonuclease Ercc 1 Xpf is required to resolve DNA interstrand cross link induced double strand breaks . ^^^ However , genetic data implicate the Ercc 1 Xpf endonuclease and proteins required for homologous recombination mediated double strand break ( DSB ) repair . ^^^ To examine the role of Ercc 1 Xpf in ICL repair , we monitored the phosphorylation of histone variant H2AX ( gamma H2AX ) . ^^^ MMC triggered sister chromatid exchanges in wild type cells but chromatid fusions in Ercc 1 ( / ) and Xpf mutant cells , indicating that in their absence , repair of DSBs is prevented . ^^^ Collectively , these data support a role for Ercc 1 Xpf in processing ICL induced DSBs so that these cytotoxic intermediates can be repaired by homologous recombination . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The xeroderma pigmentosum complementation group F ( XPF ) protein is a structure specific endonuclease in a complex with ERCC 1 and is essential for nucleotide excision repair ( NER ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The model rationalises published biochemical data and implies a novel role for the ERCC 1 subunit of eukaryotic XPF complexes . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
XPF , in association with ERCC 1 , constitutes a structure specific endonuclease that makes an incision 5 ' to the photodamage . ^^^ XPF ERCC 1 has also been implicated in both removal of interstrand DNA cross links and homology mediated recombination and in immunoglobulin class switch recombination ( CSR ) . ^^^ The complex phenotype suggests that XPF ERCC 1 is involved in multiple DNA repair processes . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Reduced levels of XPA , ERCC 1 and XPF DNA repair proteins in testis tumor cell lines . ^^^ We previously investigated 2 testis tumor cell lines with a low capacity to remove cisplatin induced DNA damage and found that they had low levels of the DNA nucleotide excision repair proteins XPA , ERCC 1 and XPF . ^^^ To determine whether low levels of XPA , ERCC 1 and XPF proteins are characteristic of testis tumor cell lines , we investigated 35 cell lines derived from cancers to determine whether groups of cell lines from diverse tissue origins differ from one another in constitutive levels of these NER proteins . ^^^ Only the 6 testis tumor cell lines showed significantly lower mean levels of XPA ( p = 0 . 001 ) , XPF ( p = 0 . 001 ) and ERCC 1 ( p = 0 . 004 ) proteins from the other groups . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Crystal structure and DNA binding functions of ERCC 1 , a subunit of the DNA structure specific endonuclease XPF ERCC 1 . ^^^ Human XPF ERCC 1 is a DNA endonuclease that incises a damaged DNA strand on the 5 ' side of a lesion during nucleotide excision repair and has additional role ( s ) in homologous recombination and DNA interstrand crosslink repair . ^^^ We show that a truncated form of XPF lacking the N terminal helicase like domain in complex with ERCC 1 exhibits a structure specific endonuclease activity with similar specificity to that of full length XPF ERCC 1 . ^^^ The XPF ERCC 1 HhH2 domain heterodimer contains two independent ssDNA binding surfaces , which are revealed by a crystal structure of the protein complex . ^^^ A crystal structure of the central domain of ERCC 1 shows its fold is strikingly similar to that of the nuclease domains of the archaeal Mus81 / XPF homologs , despite very low sequence homology . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Genotyping assays were done for ERCC 1 , ERCC 2 ( XPD ) , ERCC 4 ( XPF ) , ERCC 5 ( XPG ) , XPA , and XPC . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Three NER proteins : ERCC 1 , XPF , and XPA , are present at low levels in testis tumor cell lines , and addition of these proteins to protein extracts of testis tumor cells increases their in vitro DNA repair capacity to normal levels . ^^^ The levels of the mRNA transcripts for ERCC 1 , XPF , and XPA were measured in a panel of 14 different human cancer cell lines , using real time PCR . ^^^ The significantly lower levels of ERCC 1 , XPF , and XPA protein in testis tumor cell lines can not be explained solely by differences in transcriptional efficiency or mRNA stability . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The ERCC 1 domain folds properly only in the presence of the XPF domain , which implies a role for XPF as a scaffold for the folding of ERCC 1 . ^^^ NMR titration data show that only the ERCC 1 domain of the ERCC1 / XPF complex is involved in DNA binding . ^^^ On the basis of these findings , we propose a model for the targeting of XPF nuclease via ERCC 1 mediated interactions in the context of nucleotide excision repair . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Lower OERs have also been reported in cells deficient in XPF and ERCC 1 , which , similar to homologous recombination genes , are known to play a role in cross link repair . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The risk of OSCC was evaluated in 10 genetic polymorphisms of nine genes , such as CYP1A1 , CYP2E1 , GSTM 1 , GSTT 1 , XPA , XPC , XPC , XPF and ERCC 1 . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
ERCC 1 is an excision nuclease within the nucleotide excision repair pathway that forms a heterodimer with XPF . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
XP proteins are key players in several steps of the NER process , including DNA strand discrimination ( XPA , in complex with replication protein A ) , repair complex formation ( XPC , in complex with hHR23B ; XPF , in complex with ERCC 1 ) and repair factor recruitment ( transcription factor IIH , in complex with XPG ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Evidence for a repair enzyme complex involving ERCC 1 and complementing activities of ERCC 4 , ERCC 11 and xeroderma pigmentosum group F . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We confirmed that ERCC 1 and ERCC 4 play a role in the repair of cis diamminedichloroplatinum ( DDP ) and Melphalan ( L PAM ) induced DNA damage , while a marginal role of ERCC 2 and ERCC 3 in the cellular response to DDP and L PAM treatment has been observed . ^^^ ERCC 1 , ERCC 2 , ERCC 3 and ERCC 4 , but not ERCC 6 gene products seem to be involved in removing the lesions caused by Tallimustine and CC 1065 , minor groove alkylating agents that alkylate N 3 adenine in a sequence specific manner . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We compared the sensitivity of the wild type CHO cell line , AA 8 , with that of the CHO mutant cell lines UV 4 and UV 20 ( ERCC 1 ) , UV 5 ( ERCC 2 ) , UV 24 ( ERCC 3 ) , UV 41 ( ERCC 4 ) , UV 135 ( ERCC 5 ) , UV 61 ( ERCC 6 ) , and EM 9 ( XRCC 1 ) . ^^^ Thus mutations in the ERCC 1 and ERCC 4 genes impart a hypersensitivity to CP analogs . ^^^ CONCLUSIONS : Our findings confirm the general pattern indicating that the ERCC 1 and ERCC 4 gene products are crucial for the repair of 4HC induced DNA damage , while the other nucleotide excision repair genes examined are relatively unimportant . ^^^ These data suggest that the hypersensitivity of ERCC 1 and ERCC 4 mutants to DNA crosslinking agents may reflect a defect in recombinational repair rather than nucleotide excision repair . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The human DNA repair protein ERCC 1 resides in a complex together with the ERCC 4 , ERCC 11 and XP F correcting activities , thought to perform the 5 ' strand incision during nucleotide excision repair ( NER ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Among the NER genes , ERCC 4 and ERCC 1 are also uniquely involved in removing DNA interstrand cross linking damage . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The extract selectively fails to complement extracts of group 1 and 4 mutants consistent with the notion that the affected proteins , ERCC 1 and ERCC 4 , are part of the same complex and that mutations in one subunit also affect the other component . ^^^ These unusual group 1 and 4 mutants demonstrate that the unique UV and MMC sensitivity phenotypes displayed by these groups can be separated and support the idea that they are the result of distinct repair functions of the corresponding ERCC 1 and ERCC 4 genes : nucleotide excision repair for UV lesions and a separate repair pathway for removal of interstrand crosslinks . . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The panel of hamster cell mutants that we characterized for their relative sensitivity to fast neutrons and gamma rays includes cell lines with defects in the nucleotide excision repair pathway ; these can be further subdivided into mutants which are defective in nucleotide excision repair alone [ UV 5 ( ERCC 2 ) , UV 24 ( ERCC 3 ) , UV 135 ( ERCC 5 ) and UV 61 ( ERCC 6 ) ] compared to those which have an associated defect in the distinct but overlapping pathway for the repair of DNA crosslinks [ UV 20 ( ERCC 1 ) and UV 41 ( ERCC 4 ) ] . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Co correction of the ERCC 1 , ERCC 4 and xeroderma pigmentosum group F DNA repair defects in vitro . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
RNA expression levels of 5 FU metabolism associated genes thymidylate synthase , thymidine phosphorylase , dihydropyrimidine dehydrogenase , methylenetetrahydrofolate reductase , MAP 7 , and ELF 3 , of platinum and taxane related genes caldesmon , ERCC 1 , ERCC 4 , HER 2 / neu , and GADD 45 , and of multidrug resistance gene MRP 1 were determined using real time reverse transcriptase PCR . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We analyzed 13 polymorphisms in seven DNA repair genes belonging to different repair pathways [ 10 ray repair cross complementing group 1 ( XRCC 1 ) : 26304C > T , 26651A > G , 28152A > G ; xeroderma pigmentosum D ( XPD ) : 23591A > G , 35931A > C ; excision repair complementing defective in Chinese hamster , group 1 ( ERCC 1 ) : 19007C > T ; XRCC 3 : 4541T > C , 17893A > G , 18067C > T ; proliferating cell nuclear antigen ( PCNA ) : 6084G > C ; ERCC 4 : 30028C > T , 30147A > G ; and XRCC 2 31479A > G ] in 317 incident bladder cancer patients and 317 controls . ^^^ XRCC 3 , XRCC 1 , ERCC 4 , and XPD ERCC 1 haplotype frequencies were estimated by the maximum likelihood method . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We evaluated the influence of common genetic variation in the NER pathway on bladder cancer risk by analyzing 22 single nucleotide polymorphisms ( SNP ) in seven NER genes ( XPC , RAD23B , ERCC 1 , ERCC 2 , ERCC 4 , ERCC 5 , and ERCC 6 ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The candidate genes belong to different DNA repair pathways : base excision repair ( OGG 1 , LIG 3 , APEX , POLB , XRCC 1 , PCNA , and MUTYH ) , nucleotide excision repair ( ERCC 1 , ERCC 2 , ERCC 4 , and ERCC 5 ) , double strand breaks repair ( XRCC 2 , XRCC 3 , and XRCC 9 ) , and reversion repair ( MGMT ) genes . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
We have evaluated host cell reactivation and cell survival of wild type Chinese hamster ovary cells and of mutants in the NER genes ERCC 1 , ERCC 2 , and ERCC 4 after treatment with the methylating compounds dimethylsulfate and methylnitrosourea . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
ERCC 4 mRNA and poly ( ADP ribose ) polymerase levels were similar in both cell lines , and ERCC 1 mRNA levels were 2 to 2 . 5 fold lower in D 283 Med ( 4 HCR ) . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Selected SNPs belong to the following genes : ADH1B , ALDH 2 , APEX , CDKN2A , COMT , CYP1A1 , CYP1A2 , CYP1B1 , CYP2A6 , CYP2C19 , CYP2C9 , CYP2E1 , CYP3A4 , DRD 2 , DRD 4 , EPHX 1 , ERCC 1 , ERCC 2 , ERCC 4 , ERCC 5 , GRPR , GSTA 4 , GSTM 3 , GSTP 1 , GSTT 2 , LIG 3 , MDM 2 , MGMT , MPO , NAT 1 , NAT 2 , NQO 1 , OGG 1 , PCNA , POLB , SLC6A3 , SOD 2 , TP 53 , XRCC 1 , XRCC 2 , XRCC 3 , and XRCC 9 . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
The expressions of the 5 FU related genes TS , DPD , and TP and of the cisplatin related genes ERCC 1 , ERCC 4 , KU 80 , and GADD45A were analyzed by quantitative real time PCR . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Genes involved in DNA repair , such as XRCC 5 , ERCC 5 , ERCC 1 , RAD9A , ERCC 4 and the gene encoding DNA PK , were found to be increased to a higher level in A 549 cells than in NCI H 446 cells . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
RNA expression levels of 5 FU metabolism associated genes thymidylate synthase ( TS ) , thymidine phosphorylase ( TP ) , dihydropyrimidine dehydrogenase ( DPD ) , methylenetetrahydrofolate reductase ( MTHFR ) , MAP 7 , ELF 3 , as well as of platinum and taxane associated related genes caldesmon , excision cross complementing genes ( ERCC 1 and ERCC 4 ) HER 2 neu , DNA damage inducible gene 45 ( GADD 45 ) and multidrug resistance genes ( MDR 1 , MRP 1 ) were determined using real time RT PCR . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
Although significant homology exists between Rad 10 and the human DNA repair protein Ercc 1 in this region , we were unable to detect any interaction between Ercc 1 and Rad 1 proteins . ^^^
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q92889 and P07992	Pubmed	SVM Score :0.0
NA