| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.50565234 |
| Enhancement of damage specific DNA binding of XPA by interaction with the ERCC 1 DNA repair protein . 0.50565234^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.50175805 |
| The transfectant overexpressing mutant XPA with a defect in the interaction with either ERCC 1 , replication protein A ( RPA ) , or general transcription factor TFIIH , showed more or less decreased repair of CPD in each strand in parallel , while in the transfectant overexpressing R207G ( Arg207to Gly ) mutant XPA derived from XP 129 , a UV resistant XP12ROSV revertant , the rate of CPD repair was almost normal in each strand . 0.50175805^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Homologs of human excision repair genes ERCC 1 , XPDC / ERCC2 , and XPAC have been identified in the yeast Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Stable associations of the ERCC 1 complex with other known repair factors ( XPA , XPC , XPG and TFIIH complex ) could not be detected . . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| To study this question , we assayed malignant and nonmalignant brain tissues from 19 individuals for mRNA expression levels of the human DNA repair genes ERCC 1 , ERCC 2 , and XPAC and for differential splicing of the ERCC 1 transcript . ^^^ Furthermore , within the same individuals , malignant tissues were discordant with nonmalignant tissues for ERCC 1 and ERCC 2 , although concordance for XPAC was preserved . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Mutations in XPA that prevent association with ERCC 1 are defective in nucleotide excision repair . ^^^ The human repair proteins XPA and ERCC 1 have been shown to be absolutely required for the incision step of nucleotide excision repair , and recently we identified an interaction between these two proteins both in vivo and in vitro ( L . ^^^ The ERCC 1 binding domain on XPA was previously mapped to a region containing two highly conserved XPA sequences , Gly 72 to Phe 75 and Glu 78 to Glu 84 , which are termed the G and E motifs , respectively . ^^^ In vitro , the binding of ERCC 1 to delta E was reduced by approximately 70 % , and binding to delta G was undetectable ; furthermore , both mutants failed to complement XPA cell extracts in an in vitro DNA repair synthesis assay . ^^^ These results suggest that the association between XPA and ERCC 1 is a required step in the nucleotide excision repair pathway and that the probable role of the interaction is to recruit the ERCC 1 incision complex to the damage site . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Messenger RNA levels of XPAC and ERCC 1 in ovarian cancer tissue correlate with response to platinum based chemotherapy . ^^^ The rate limiting step , DNA damage recognition and excision , is effected by the protein products of the genes ERCC 1 and XPAC . ^^^ We therefore assessed mRNA levels of ERCC 1 and XPAC in malignant ovarian cancer tissues from 28 patients that were harvested before the administration of platinum based chemotherapy . ^^^ Cancer tissues from patients whose tumors were clinically resistant to therapy ( n = 13 ) showed greater levels of total ERCC 1 mRNA ( P = 0 . 059 ) , full length transcript of ERCC 1 mRNA ( P = 0 . 026 ) , and XPAC mRNA ( P = 0 . 011 ) , as compared with tumor tissues from those individuals clinically sensitive to therapy ( n = 15 ) . ^^^ These data suggest greater activity of the DNA excision repair genes ERCC 1 and XPAC in ovarian cancer tissues of patients clinically resistant to platinum compounds . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| ERCC 1 and XPBC / ERCC 3 ) , the mouse XPAC promoter region lacks classical promoter elements like TATA , GC and CAAT boxes . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Specific association between the human DNA repair proteins XPA and ERCC 1 . ^^^ Here , we demonstrate both in vivo , using the two hybrid system , and in vitro , using recombinant proteins , that the human repair factors XPA and ERCC 1 specifically interact . ^^^ In addition , we report an initial determination of the domains in ERCC 1 and XPA that mediate this interaction . ^^^ These results suggest that XPA may play a role in the localization or loading of an incision complex , composed of ERCC 1 and possibly other repair factors , onto a damaged site . . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Formation of a ternary complex by human XPA , ERCC 1 , and ERCC 4 ( XPF ) excision repair proteins . ^^^ The XPA bound fraction complemented cell free extracts of excision repair cross complementing 1 ( ERCC 1 ) , ERCC 4 ( XP F ) , and XP A mutants . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| To study the influence of nucleotide excision repair ( NER ) on mutagenesis in vivo , ERCC 1 + / , XPA / , and wild type ( ERCC1+ / + and XPA+ / + , respectively ) lambda lacZ transgenic mice were treated i . p . with N hydroxy 2 acetylaminofluorene ( N OH AAF ) and lacZ mutant frequencies were determined in liver . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Based on these results , a model for MMC crosslink recognition is proposed in which ERCC 1 and XPA each participate in formation of one or more multimeric complexes on the crosslinked DNA and XPA also aids in the formation , but is not a component of a higher molecularweight multimeric complex that may contain ERCC 1 . . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| XPA , an essential protein in nucleotide excision repair ( NER ) , interacts with damaged DNA and other proteins ( RPA , ERCC 1 and TFIIH ) to remove a wide variety of chemically and structurally distinct DNA lesions from the eukaryotic genome . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| ERCC 1 also binds to XPA protein to make a large protein complex at the site of DNA damage . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Of the nine ERCC 1 mutant alleles characterized two mutations were identified in the XpA binding region of the Ercc 1 protein ; no mutations were found in the N terminal portion of the Ercc 1 protein . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Reconstitution of mammalian excision repair activity with mutant cell free extracts and XPAC and ERCC 1 proteins expressed in Escherichia coli . ^^^ XPAC and ERCC 1 were expressed as fusion proteins with the Escherichia coli maltose binding protein ( MBP ) and purified to > 80 % homogeneity by affinity chromatography . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Sequential binding of DNA repair proteins RPA and ERCC 1 to XPA in vitro . ^^^ The xeroderma pigmentosum group A protein ( XPA ) was shown to bind to the replication protein A ( RPA ) or the excision repair cross complementing rodent repair deficiency group 1 protein ( ERCC 1 ) , and these interactions might be involved in the damage recognition and / or incision steps , of nucleotide excision repair . ^^^ These regions do not overlap with the ERCC 1 binding region of XPA , and a ternary protein complex of RPA , XPA and ERCC 1 was detected in vitro . ^^^ In addition , using the surface plasmon resonance biosensor , the binding of RPA and ERCC 1 to XPA was investigated . ^^^ The dissociation constants ( KD ) of RPA and ERCC 1 with XPA were 1 . 9 10 10 ( 8 ) and 2 . 5 10 10 ( 7 ) M , respectively . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Furthermore , reconstitution of the human excision nuclease was observed with a mixture of five repair factors ( XPA , XPC , XPG , TFIIH , and RPA ) and the recombinant XPF ERCC 1 , thus verifying that no additional protein factors are needed for the specific dual incisions characteristic of human excision repair . . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The XPA protein was not co immunoprecipitated with ERCC 1 in any cell strain . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| In this study , the mRNA levels of the different genes involved in NER ( ERCC 1 , XPA , XPB , XPC , XPD , XPF ) were examined in a panel of eight different human cancer cell lines , together with the overall DNA repair capacity using a host cell reactivation assay of a damaged plasmid . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The protein XPA appears to play a central role in NER , recognizing and / or verifying damaged DNA and recruiting other proteins , including RPA , ERCC 1 , and TFIIH , to repair the damage . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The resultant NER complex is a cooperative assembly of XPF , ERCC 1 , XPA , RPA , and XPG subunits . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Cell cycle checkpoint abrogator UCN 01 inhibits DNA repair : association with attenuation of the interaction of XPA and ERCC 1 nucleotide excision repair proteins . ^^^ Because mammalian cells remove CDDP induced DNA adducts through the nucleotide excision repair ( NER ) pathway , we determined the effects of UCN 01 on NER by measuring its effects on the interaction of the repair factors XPA and ERCC 1 and the phosphorylation / dephosphorylation of the repair proteins . ^^^ Although expression of XPA and ERCC 1 proteins was elevated in cells exposed to UCN 01 , the treatment resulted in a decreased ERCC 1 level in the Triton 10 100 insoluble fraction of cell lysates . ^^^ A pull down assay using the MBP XPA fusion protein showed a significant reduction in the binding of ERCC 1 to XPA in nuclear extracts from UCN 01 treated cells compared with untreated cells , suggesting that UCN 01 reduced the XPA ERCC 1 interaction . ^^^ In vitro phosphorylation revealed that UCN 01 had no effect on the phosphorylation / dephosphorylation status of either XPA or ERCC 1 ; however , UCN 01 caused dephosphorylation of an unidentified XPA bound protein with an apparent molecular mass of 52 kDa . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We have previously reported on mRNA expression of ERCC 1 , XPA and XPD in human ovarian cancer cells and tissues . ^^^ In each of these cell lines , the genes examined included ERCC 1 , XPA , XPB , XPD , XPG , CSB and p 53 . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Human XPA , a 31 kDa , zinc associated protein , is thought to play a major NER role in the recognition of damaged DNA and the recruitment of other proteins , including RPA , ERCC 1 , and TFIIH , to repair the damage . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Such genes include ERCC 1 , XPA , XPB , XPD , XPF , and XPG . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Included in this assessment are ERCC 1 , XPB , CSB , and XPA , studied in 28 ovarian cancer tumor tissue specimens . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We have used the same study persons to investigate the correlation between expression of eight genes involved in nucleotide excision repair and DNA repair capacity . mRNA levels of XPA , XPB , XPC , XPD , XPF , XPG , CSB and ERCC 1 in primary lymphocytes from 33 individuals were quantified by dot blots and normalized to beta actin . ^^^ ERCC 1 and XPD mRNA quantities were highly correlated ( r=0 . 89 ; P < 10 ( 11 ) ) while XPA , XPB , XPC , XPG , XPFand CSB mRNAs were moderately correlated ( r=0 . 2 0 . 7 ) . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Since these platinum resistant tumors also show higher mRNA levels of ERCC 1 and XPA , platinum resistance appears to be associated with concurrent up regulation of four genes ( XPA , ERCC 1 , XPB , and CSB ) . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Cisplatin sensitivity has been shown to be dependent on the expression of the two DNA repair proteins ERCC 1 and XPA . ^^^ Therefore , these studies were undertaken to determine if hypoxia activated TPZ pretreatment inhibited the cells ' normal protective response to cisplatin via inhibiting the upregulation of ERCC 1 and / or XPA expression . ^^^ Expression of ERCC 1 and XPA were evaluated at the mRNA and protein level at 24 or 48 h after drug exposure . ^^^ CONCLUSION : In contrast to reports in the literature , this study did not demonstrate cisplatin inducing its own repair by upregulating the DNA crosslink repair proteins ERCC 1 or XPA . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We have made a replication defective adenovirus , AdCMV FlagXPA ( 59 114 ) , that expresses a truncated form of XPA encompassing amino acids 59 114 sufficient for binding to the excision repair cross complementing protein 1 ( ERCC 1 ) / xeroderma pigmentosum complementation group F ( XPF ) nuclease essential for making an incision 5 ' of the damage . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Moreover , a decrease of the expression of two proteins of the nucleotide excision repair ( NER ) system , ERCC 1 and XPA , was observed . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The steady state levels of ERCC 1 mRNA , but not of XPA , were moderately elevated in the resistant cells . ^^^ Exposure to either one of the drugs resulted in an induction of XPA in all the cell lines and of ERCC 1 in cisplatin resistant cells . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis revealed age associated decreases of 40 % 80 % between newborn and old adult donor cells in the constitutive protein levels of p 53 , p 21 , XPA , RPA , ERCC 1 , and PCNA . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Several NER genes in this pathway including XPB , XPD , XPA and ERCC 1 have been implicated in anticancer drug resistance in human tumor cells . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We have recently completed screening of the National Cancer Institute human tumor cell line panel and demonstrated that among four nucleotide excision repair proteins ( XPA , XPB , XPD , and ERCC 1 ) , only the TFIIH subunit XPD endogenous protein levels correlate with alkylating agent drug resistance . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Several NER genes in this system including XPA , XPB , ERCC 1 , and ERCC 2 ( XPD ) have been implicated in anticancer drug resistance in human tumor cells . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Subsequently Xpa and Xpc knockouts have proved to be good models for the human NER deficiency disease , xeroderma pigmentosum , leading to speculation that the recombination , rather than the NER deficit is the key to the Ercc 1 knockout phenotype . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The determination of ERCC 1 and XPA expression , two enzymes of the NER pathway , by reverse transcriptase polymerase chain reaction ( RT PCR ) , demonstrated that ERCC 1 expression was predictive of L OHP sensitivity ( r ( 2 ) =0 . 67 , P=0 . 02 ) and XPA level after oxaliplatin exposure was also correlated to L OHP IC ( 50 ) ( r ( 2 ) =0 . 5 ; P=0 . 04 ) . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Mutations in ERCC 1 , XPA , XPB , XPC , XPF , XPG and CSB dramatically reduced TER . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Bulky DNA lesions are mainly repaired by nucleotide excision repair ( NER ) , in which the interaction of ERCC 1 with XPA protein recruits the ERCC 1 XPF complex , which acts as a structure specific endonuclease in the repair process . ^^^ However , additional functions besides NER have been suggested for the ERCC 1 XPF complex , because ERCC 1 or XPF deficient rodent cells are significantly more sensitive to DNA interstrand cross linking ( ICL ) agents such as cis diamminedichloroplatinum ( 2 ) ( CDDP ) than any other NER deficient cells and because ERCC 1 deficient mice suffer a more severe phenotype than XPA deficient mice . ^^^ By using RNA interference we show here that suppression of ERCC 1 expression increases the sensitivity of xeroderma pigmentosum group A ( XPA ) deficient human cells to CDDP but not to UV . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Reduced levels of XPA , ERCC 1 and XPF DNA repair proteins in testis tumor cell lines . ^^^ We previously investigated 2 testis tumor cell lines with a low capacity to remove cisplatin induced DNA damage and found that they had low levels of the DNA nucleotide excision repair proteins XPA , ERCC 1 and XPF . ^^^ To determine whether low levels of XPA , ERCC 1 and XPF proteins are characteristic of testis tumor cell lines , we investigated 35 cell lines derived from cancers to determine whether groups of cell lines from diverse tissue origins differ from one another in constitutive levels of these NER proteins . ^^^ Only the 6 testis tumor cell lines showed significantly lower mean levels of XPA ( p = 0 . 001 ) , XPF ( p = 0 . 001 ) and ERCC 1 ( p = 0 . 004 ) proteins from the other groups . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Inhibition of nucleotide excision repair by anti XPA monoclonal antibodies which interfere with binding to RPA , ERCC 1 , and TFIIH . ^^^ The xeroderma pigmentosum group A protein ( XPA ) binds to three nucleotide excision repair ( NER ) factors : RPA , ERCC 1 , and TFIIH . ^^^ The two clones inhibited the binding of XPA to RPA , ERCC 1 , and TFIIH . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The specific polymorphic loci examined include XPA [ A / G at 5 ' untranslated region ( UTR ) ] , XPC ( poly AT , Ala ( 499 ) Val , Lys ( 939 ) Gln ) , XPD ( Asp ( 312 ) Asn , Lys ( 751 ) Gln ) , XPG ( His ( 1104 ) Asp ) , ERCC 1 ( G / T at 3 ' UTR ) , and ERCC 6 ( Met ( 1097 ) Val , Arg ( 1230 ) Pro ) . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Three NER proteins : ERCC 1 , XPF , and XPA , are present at low levels in testis tumor cell lines , and addition of these proteins to protein extracts of testis tumor cells increases their in vitro DNA repair capacity to normal levels . ^^^ The levels of the mRNA transcripts for ERCC 1 , XPF , and XPA were measured in a panel of 14 different human cancer cell lines , using real time PCR . ^^^ The significantly lower levels of ERCC 1 , XPF , and XPA protein in testis tumor cell lines can not be explained solely by differences in transcriptional efficiency or mRNA stability . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The gene expression of the repair enzymes , XPA and XRCC 1 , was the same in all 3 cell lines but ERCC 1 , ERCC 3 and XPC were 2 6 times higher in AG 6000 compared to A 2780 cells . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| The risk of OSCC was evaluated in 10 genetic polymorphisms of nine genes , such as CYP1A1 , CYP2E1 , GSTM 1 , GSTT 1 , XPA , XPC , XPC , XPF and ERCC 1 . ^^^ CONCLUSION : CYP2E1 , XPA and ERCC 1 polymorphisms may affect the risk of OSCC . . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We used the allelic discrimination method to identify polymorphisms in GSTT 1 , SULT1C2 , CDA , SXR ( drug metabolic pathways ) , XPD , XPA , XPG , ERCC 1 , TOP2A ( DNA repair ) , VEGF ( angiogenesis ) , and MDR 1 ( multidrug resistance ) genes in 110 adult patients with intermediate risk AML , enrolled in the CETLAM 99 prospective trial . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| METHODS : Expression of CSA , CSB , XPC , hHR23B , XPA , XPB , ERCC 1 and p 53 genes was analyzed by quantitative RT PCR and immunoblotting in 26 HCC and 9 normal livers . ^^^ XPA , XPC , hHR23B and ERCC 1 mRNA levels were significantly increased ( p < 0 . 05 ) in HCC arising in cirrhotic livers compared to non fibrotic tissue . ^^^ Moreover , expression of ERCC 1 , XPA and XPC mRNA was significantly augmented in HCC , even more in tumors arising in cirrhotic liver . ^^^ ERCC 1 , XPC ad XPA mRNA levels were highly correlated in NT and HCC . ^^^ |
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| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We investigated 44 single nucleotide polymorphisms ( SNPs ) in 20 DNA repair genes including nucleotide excision repair ( NER ) genes XPA , ERCC 1 , ERCC2 / XPD , ERCC4 / XPF and ERCC5 / XPG ; base excision repair ( BER ) genes APE1 / APEX , OGG 1 , MPG , XRCC 1 , PCNA , POLB , POLiota , LIG 3 and EXO 1 ; double strand break repair ( DSB R ) genes XRCC 2 , XRCC 3 , XRCC 9 , NBS 1 and ATR ; and direct damage reversal ( DR ) gene MGMT / AGT . ^^^ Our results indicate that SNPs in the NER genes ERCC 1 ( Asn118Asn , 15310G > C , 8902G > T ) , XPA ( 4G > A ) , ERCC2 / XPD ( Lys751Gln ) and ERCC5 / XPD ( His46His ) ; the BER genes APE1 / APEX ( Ile64Val ) , OGG 1 ( Ser326Cys ) , PCNA ( 1876A > G ) and XRCC 1 ( Arg194Trp , Arg280His , Arg399Gln ) ; and the DSB R genes ATR ( Thr211Met ) , NBS 1 ( Glu185Gln ) , XRCC 2 ( Arg188His ) and XRCC 9 ( Thr297Ile ) modulate NSCLC risk . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| Genotyping assays were done for ERCC 1 , ERCC 2 ( XPD ) , ERCC 4 ( XPF ) , ERCC 5 ( XPG ) , XPA , and XPC . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| XP proteins are key players in several steps of the NER process , including DNA strand discrimination ( XPA , in complex with replication protein A ) , repair complex formation ( XPC , in complex with hHR23B ; XPF , in complex with ERCC 1 ) and repair factor recruitment ( transcription factor IIH , in complex with XPG ) . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| XPA versus ERCC 1 as chemosensitising agents to cisplatin and mitomycin C in prostate cancer cells : role of ERCC 1 in homologous recombination repair . ^^^ In this study , we used synthetic siRNAs targeted to XPA and ERCC 1 and compared their effectiveness in sensitising mismatch repair deficient prostate cancer cell lines to cisplatin and mitomycin C . ^^^ Excision repair of cisplatin adducts in PC 3 cells was attenuated to a similar extent by XPA and ERCC 1 downregulation . ^^^ Downregulation of XPA but not ERCC 1 caused an increase in the number of cisplatin induced RAD 51 foci in PC 3 cells , suggesting that HRR is able to substitute for NER in these cells . ^^^ These results indicate that ERCC 1 is a broader therapeutic target than XPA with which to sensitise cancer cells to chemotherapy because of its additional role in recombination repair . . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| We have now determined genotypes for seven single nucleotide polymorphisms in five nucleotide excision repair genes , including Xeroderma pigmentosum complementation group A ( XPA +62T > C ) , group C ( XPC Lys939Gln and Ala499Val ) , group D ( XPD Asp312Asn and Lys751Gln ) , and group G ( XPG His1104Asp ) and ERCC 1 ( 8092 C > A ) in a total of 160 sister pairs for whom DRC phenotype data were available . ^^^ |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07992 and P23025 |
Pubmed |
SVM Score :0.0 |
| NA |
|