| Interacting proteins: Q13480 and P07949 |
Pubmed |
SVM Score :0.0 |
| In response to GDNF stimulation , SHC bound to GAB 1 and GRB 2 adaptor proteins as well as RET , and SHC and GAB 1 were highly phosphorylated on tyrosine . ^^^ The complex formation consisting of SHC , GAB 1 and GRB 2 was almost abolished by replacement of tyrosine 1062 in RET with phenylalanine . ^^^ |
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| Interacting proteins: Q13480 and P07949 |
Pubmed |
SVM Score :0.0 |
| Whereas SHC bound to RET was associated with GRB 2 and GAB 1 proteins , SNT / FRS2 was associated with GRB 2 only , suggesting that SNT / FRS2 is involved mainly in the activation of the RAS / mitogen activated protein kinase ( MAPK ) pathway but not the phosphatidylinositol 3 kinase ( PI 3 K ) / AKT pathway . ^^^ |
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| Interacting proteins: Q13480 and P07949 |
Pubmed |
SVM Score :0.0 |
| Biochemical and biological responses induced by coupling of Gab 1 to phosphatidylinositol 3 kinase in RET expressing cells . ^^^ We previously reported that tyrosine 1062 in RET receptor tyrosine kinase activated by glial cell line derived neurotrophic factor ( GDNF ) represents a binding site for the Shc Grb 2 Gab1 complex , and that the p 85 subunit of phosphatidylinositol 3 kinase ( PI3K ) and SHP 2 tyrosine phosphatase is associated with Gab 1 in GDNF treated cells . ^^^ In the present study , we further analyzed the physiological roles of Gab 1 downstream of RET , using Gab 1 mutants that lack the binding sites for PI3K ( Gab 1 PI3K m ) or SHP 2 ( Gab 1 SHP2 m ) . ^^^ Expression of Gab 1 PI3K m in SK N MC human primitive neuroectodermal tumor cells expressing wild type RET markedly impaired Akt phosphorylation , Rac 1 activation , and lamellipodia formation that were induced by GDNF whereas expression of Gab 1 SHP2 m partially impaired Erk activation . ^^^ Furthermore , expression of Gab 1 PI3K m , but not Gab 1 SHP2 m , in TT human medullary thyroid carcinoma cells expressing RET with a multiple endocrine neoplasia 2A mutation enhanced cytochrome c release , and apoptosis induced by etoposide , suggesting that PI3K is involved in survival of TT cells via a mitochondrial pathway . ^^^ |
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| Interacting proteins: Q13480 and P07949 |
Pubmed |
SVM Score :0.0 |
| RAI ( ShcC / N Shc ) dependent recruitment of GAB 1 to RET oncoproteins potentiates PI 3 K signalling in thyroid tumors . ^^^ This association is constitutive , but , in the presence of RET oncoproteins , both RAI and GAB 1 are tyrosine phosphorylated , and the stoichiometry of this interaction remarkably increases . ^^^ |
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| Interacting proteins: Q13480 and P07949 |
Pubmed |
SVM Score :0.0 |
| NA |
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