| One of these kinases , Lyn , was activated by hypotonic stress and phosphorylated TRPV 4 in an immune complex kinase assay and an in vitro kinase assay using recombinant Lyn and TRPV 4 . ^^^ Transfection of wild type Lyn dramatically potentiated hypotonicity dependent TRPV 4 tyrosine phosphorylation whereas dominant negative acting Lyn modestly inhibited it . ^^^ |