| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| Translocation of the Csk homologous kinase ( Chk / Hyl ) controls activity of CD 36 anchored Lyn tyrosine kinase in thrombin stimulated platelets . ^^^ Chk and Lyn , but not Csk and c Src , co fractionated in the higher density lysate fractions of resting platelets , with Chk being found to localize close to CD 36 ( membrane glycoprotein 4 ) anchored Lyn . ^^^ In vitro phosphorylation assays showed that Chk suppressed Lyn activity by phosphorylating its C terminal negative regulatory tyrosine . ^^^ Upon stimulation of platelets with thrombin , the rapid and complete translocation of Chk away from Lyn caused concomitant activation of Lyn . ^^^ These results suggest that Chk , but not Csk , may function as a translocation controlled negative regulator of CD 36 anchored Lyn in thrombin induced platelet activation . . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| The Csk homologous kinase ( Chk ) , which is co expressed with C terminal Src kinase ( Csk ) in hematopoietic cells , negatively regulates Src family kinases in vitro with selectivity toward Lyn but not c Src in platelets . ^^^ FN induced cell spreading accompanied a sustained increase in Lyn activity with coincidental kinetics and the activation of Lyn was also suppressed by overexpression of Chk but not a Chk mutant lacking the SH 3 domain . ^^^ These results suggest that sustained activation of Lyn , which is regulated by membrane anchored Chk , plays a crucial role in VLA 5 mediated cell spreading but not cell attachment to a FN substrate . . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| CHK down regulates SCF / KL activated Lyn kinase activity in Mo7e megakaryocytic cells . ^^^ Studies using immobilized c Kit phosphopeptides show that Lyn is able to specifically associate with the tyrosine phosphorylated juxtamembrane 568Y * VY * IDPT sequence of c Kit which has previously been shown to associate with CHK . ^^^ In cells over expressing CHK by means of a recombinant vaccinia virus , we observed an elimination of the SCF / KL stimulated Lyn kinase peak of activity observed at 2 5 minutes in cells infected with the helper T 7 expressing vaccinia virus by itself . ^^^ Taken together , these findings show that CHK is able to down regulate SCF / KL stimulated Lyn activity in megakaryocytes . . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| We observed that in MCF 7 CHK ( wt ) cells Lyn kinase activity was more profoundly inhibited than Src kinase activity . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| First , CHK directly binds to the SFK members Hck , Lyn , and Src to form stable protein complexes . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| In vitro kinase assays showed that Chk overexpression suppressed the tyrosine kinase activity of Lyn , a member of the Src family , immunoprecipitated from Triton 10 100 lysates . ^^^ Subcellular fractionation studies revealed that fractions of Chk and Lyn , resistant to Triton 10 100 solubilization , are associated with mitotic chromosome scaffolds and spindles . ^^^ Chk overexpression induced a decrease in autophosphorylation of Lyn and concomitant changes in levels of tyrosine phosphorylation of proteins associated with both fractions . ^^^ These results indicate that Chk , Lyn and the tyrosine phosphorylated proteins localize to mitotic chromosomes and spindles , suggesting that Chk dependent tyrosine phosphorylation , presumably through Lyn , may be involved in chromosome dynamics . . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CHK down regulated c Src and Lyn protein expression and kinase activity , and the entry into mitosis was delayed in the wt CHK transfected MCF 7 cells upon HRG treatment . ^^^ |
|
| Interacting proteins: P42679 and P07948 |
Pubmed |
SVM Score :0.0 |
| NA |
|