Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
NA |
|
Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.93333749 |
Furthermore , coimmunoprecipitations using anti Igalpha Ab demonstrated that Lyn associated with the resting BCR was constitutively phosphorylated and activated in CD 45 negative cells . 0.93333749^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.91147801 |
An association between CD 45 and the Src family protein tyrosine kinase , Lyn , was maintained in the presence of IL 6 ; the formation of the CD45 / Lyn complex was impaired by vanadate . 0.91147801^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Receptor engagement also resulted in the rapid association of Lyn with the phosphotyrosine phosphatase , CD 45 . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Data show that cross linking of CD 45 markedly inhibits CD40L induced activity of the Src family kinases Lck and Lyn . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
We previously demonstrated that CD 45 negatively regulates Lyn in the WEHI 231 B cell line . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Selective regulation of Lyn tyrosine kinase by CD 45 in immature B cells . ^^^ The results clearly demonstrated that among PTKs examined ( Lyn , Lck , and Syk ) , only Lyn kinase is dysregulated in the absence of CD 45 such that without B cell antigen receptor ligation , Lyn is hyperphosphorylated and activated in CD 45 negative clones . ^^^ Thus , Lyn seems to be a selective in vivo substrate for CD 45 in immature B cells . . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Additionally , CD 45 was selectively associated with the src family protein tyrosine kinase , lyn . ^^^ This finding suggests that CD 45 may preferentially regulate the phosphorylation of lyn and thus , its activity . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
The B cell src type tyrosine kinase lyn , which is activated following CD 40 engagement , is a potential target for the effects of CD 45 observed in our experiments , because CD45 / CD40 crosslinking resulted in the inhibition of CD 40 mediated lyn phosphorylation and activation . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Signal transduction and glycophosphatidylinositol linked proteins ( lyn , lck , CD 4 , CD 45 , G proteins , and CD 55 ) selectively localize in Triton insoluble plasma membrane domains of human leukemic cell lines and normal granulocytes . ^^^ In the present study , we observed lck , lyn , src , hck , CD 4 , CD 45 , G proteins , and CD 55 ( decay accelerating factor ) expression in the buoyant low density Triton insoluble ( LDTI ) fraction of selected leukemic cell lines and granulocytes . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
CD 45 modulates phosphorylation of both autophosphorylation and negative regulatory tyrosines of Lyn in B cells . ^^^ It has been shown that Src family tyrosine kinases such as Lyn could be a potential substrate for CD 45 . ^^^ We report here that both autophosphorylation and C terminal negative regulatory tyrosines of Lyn were hyperphosphorylated in CD 45 deficient DT 40 B cells . ^^^ Consistent with this observation , Lyn activation upon receptor ligation was profoundly decreased in CD 45 deficient cells . ^^^ Taken together , our results suggest that dephosphorylation of tyrosine residues at both autophosphorylation and negative regulatory sites is mediated by CD 45 in vivo , and that dephosphorylation of C terminal tyrosine is a prerequisite for participation of Lyn in BCR signaling . . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Syk , but not Lyn , recruitment to B cell antigen receptor and activation following stimulation of CD 45 B cells . ^^^ In contrast to CD45+ cells , Lyn exhibited high basal tyrosine phosphorylation in the CD 45 cells and was not further phosphorylated upon Ag stimulation . ^^^ Moreover , CD79a ITAM phosphopeptides failed to bind Lyn from the CD 45 cells . ^^^ Thus , Y 508 phosphorylation of Lyn occurs in the absence of cellular CD 45 expression and appears to render the kinase unable to associate with the phosphorylated receptor complex via its Src homology 2 domain and to participate in signal propagation . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
RESULTS : We found that CD 45 colocalized with beta 2 integrin and the Src family kinase p53 / 56 ( lyn ) to adhesion sites in bone marrow derived macrophages . ^^^ In adherent macrophages , absence of CD 45 led to the hyperphosphorylation and hyperactivation of p56 / 59 ( hck ) and p53 / 56 ( lyn ) , but not of p 58 ( c fgr ) . ^^^ Furthermore , coexpression of CD 45 with p 59 ( hck ) or p 56 ( lyn ) containing a tyrosine to phenylalanine mutation at the carboxy terminal negative regulatory site resulted in decreased tyrosine phosphorylation of the Src family member kinases due to dephosphorylation of the potentiating tyrosine phosphorylation site within the kinase domain . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Cross linking of either class 2 molecules or the BCR on CD 45 positive K 46 17micromlambda B lymphoma cells was observed to induce activation of the Src family protein tyrosine kinase Lyn , tyrosine phosphorylation of Syk and phospholipase Cgamma , and the production of inositol 1 , 4 , 5 trisphosphate leading to intracellular mobilization as well as extracellular influx of Ca2+ . ^^^ In the absence of CD 45 , cross linking of either class 2 molecules or the BCR failed to induce activation of Lyn . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
The regulation of calcium responses involves a network of tyrosine kinases ( e . g . lyn , syk ) , tyrosine or lipid phosphatases ( CD 45 , SHP 1 , SHIP ) , and accessory molecules ( CD21 / CD19 , CD 22 , FcR gamma 2b ) . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
They are currently being used as a well defined and unlimited cell source to study antigen receptor signalling and B cell specific gene regulation , and the dependence of these processes on the products of the vav , CD 45 , lyn , oct 2 , btk and OBF 1 genes . . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Thus , we propose that , although not indispensable in mast cell degranulation , CD 45 positively regulates the signaling through FcepsilonRI by promoting the activation of FcepsilonRI associated Lyn . . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Our results demonstrate that the tyrosine phosphatase CD 45 is the receptor for GAL 1 , and that the src type tyrosine kinase Lyn is a target for the effects of GAL1 / CD45 interactions in B cells . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Compared with the effects in the same system of null mutations in other BCR signaling elements , such as CD 45 and Lyn kinase , these results indicate that Syk is essential for transmitting a signal that initiates the program of B lymphocyte maturation . . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
The results were as follows : ( 1 ) when solubilized cell membrane lysates were affinity bound to immobilized GAL 1 and eluted by competition , the tyrosine phosphatase glyco protein CD 45 was found in the eluate , highlighting the role of CD 45 as a receptor of GAL 1 ; ( 2 ) the phosphatase activity of cell membranes diminished after incubation with GAL 1 ; ( 3 ) immunoprecipitation experiments demonstrated that the phosphotyrosine kinase Lyn was dysregulated in cells that have been cultured in medium containing 700 nM GAL 1 , and ( 4 ) that the ratio between two isoforms of Lyn was modified during the treatment with GAL 1 . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
This fraction appears to be important for signaling because it is enriched for lyn kinase but lacks CD 45 tyrosine phosphatase and because the BCR that moves into this domain becomes more highly phosphorylated . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
BCR signaling in mature B cells was shown to be initiated by the translocation of the BCR into cholesterol and sphingolipid enriched membrane microdomains that include the Src family kinase Lyn and exclude the phosphatase CD 45 . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Since in normal B cells Lyn and SHP 1 act in concert within a common negative pathway in which CD 45 counteracts SHP 1 regulatory role , we propose that this feedback regulatory pathway is crippled to different degrees in human SLE B cells . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
CD 45 tyrosine phosphatase inhibits erythroid differentiation of umbilical cord blood CD34+ cells associated with selective inactivation of Lyn . ^^^ These results indicate selective inactivation of Lyn by CD 45 activated with NU L ( PAN ) and could partly explain the inhibitory mechanism on erythropoiesis exhibited by EPO . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
In both CD 45 and CD45+ U 266 cells , STAT 3 and MAPK ( ERK1 / 2 ) can be activated in response to IL 6 equally between them , but src family kinases such as Lyn , Fyn can be activated only in CD45+ U 266 cells . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
We further confirmed that the translocated CD 45 bound to IL 6Ralpha , Lyn , and flotillin 2 , and this was followed by the dephosphorylation of the negative regulatory Tyr 507 of Lyn . ^^^ |
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Interacting proteins: P08575 and P07948 |
Pubmed |
SVM Score :0.0 |
Microglial activation might involve beta amyloid binding and activation of cell surface immune and adhesion molecules such as CD 45 , CD 40 , CD 36 and integrins , with the subsequent recruitment of Src family tyrosine kinases such as Fyn , Lyn and Syk kinases . ^^^ |
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