| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Effective activation of the proenzyme form of the urokinase type plasminogen activator ( pro uPA ) by the cysteine protease cathepsin L . ^^^ Increased levels of both the cysteine protease , cathepsin L , and the serine protease , uPA ( urokinase type plasminogen activator ) , are present in solid tumors and are correlated with malignancy . uPA is released by tumor cells as an inactive single chain proenzyme ( pro uPA ) which has to be activated by proteolytic cleavage . ^^^ We analyzed in detail the action of the cysteine protease , cathepsin L , on recombinant human pro uPA . ^^^ Enzymatic assays , SDS PAGE and Western blot analysis revealed that cathepsin L is a potent activator of pro uPA . ^^^ As determined by N terminal amino acid sequence analysis , activation of pro uPA by cathepsin L is achieved by cleavage of the Lys 158 Ile159 peptide bond , a common activation site of serine proteases such as plasmin and kallikrein . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Cysteine proteases ( cathepsin B [ CATB ] and cathepsin L [ CATL ] ) , the serine protease urokinase type plasminogen activator ( UPA ) , and plasminogen activator inhibitor type 1 ( PAI 1 ) are thought to play an important part in cancer invasion and metastasis . ^^^ The aims of this study were to measure CATB , CATL , UPA , and PAI 1 in gastric cancer ( GC ) and normal mucosa distant from the tumor ( NORM ) ; to evaluate whether tissue levels are related to tumor stage , grade , or histotype ; to assess their prognostic relevance ; and to examine UPA and PAI 1 expression immunohistochemically . ^^^ NORM ( CATB , CATL , UPA , PAI 1 ) tissues ; ( 2 ) in GC with versus without metastasis ( CATB , CATL , UPA ) ; ( 3 ) in poorly or moderately versus well differentiated GC ; and ( 4 ) in diffuse versus intestinal type GC ( CATB , CATL ) . ^^^ These results confirm the important role of CATB , CATL , UPA and PAI 1 in gastric cancer progression . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| At both levels , concentrations of uPA were significantly correlated with those for uPAR . uPA levels also correlated significantly with cathepsin B and cathepsin D but not with cathepsin L , MMP 8 or MMP 9 levels . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Cysteine proteases [ cathepsin B ( CATB ) , cathepsin L ( CATL ) ] , the serine protease urokinase type plasminogen activator ( UPA ) and its inhibitor type 1 ( PAI 1 ) play an important part in cancer invasion . ^^^ AIMS : To determine CATB , CATL , UPA , PAI 1 in chronic atrophic gastritis , intestinal metaplasia and gastric epithelial dysplasia , as precancerous changes , and to compare these data with those obtained in gastric cancer . ^^^ RESULTS : CATB , CATL , UPA and PAI 1 were significantly higher in chronic atrophic gastritis than in controls ( CATB : P < 0 . 001 ; CATL : P < 0 . 005 ; UPA : P < 0 . 000001 ; PAI 1 : P < 0 . 005 ) . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Phenotype rasuPA+ / CL is dependent on expression of the serine type protease urokinase plasminogen activator ( uPA ) and the phenotype rasCL+ / uPA on the cystine type protease cathepsin L ( CL ) for lung colonization in experimental metastasis . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| To evaluate the relationship between tumor biological factors and the metastatic potential of primary breast cancer , proteolytic factors uPA , PAI 1 , and cathepsin L , which are associated with tumor invasion and metastasis , were determined in breast cancer tissue extracts by ELISA and the values assessed by uni and multivariate analysis as well as CART ( classification and regression trees ) in comparison with traditional prognostic factors . ^^^ Cysteine protease cathepsin L , serine protease uPA , and the protease inhibitor PAI 1 were determined by ELISA in extracts of primary tumors of 103 node negative breast cancer patients and values assessed by univariate and multivariate analysis in comparison with traditional prognostic factors ( tumor size , steroid hormone receptor status , grading , vessel invasion , menopausal status ) . ^^^ PAI 1 , cathepsin L , tumor size , grading , and steroid hormone receptor status but not uPA , vessel invasion , and menopausal status were of prognostic relevance for disease free survival ( univariate analysis ) . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| However , inhibition of JNK kinase ( JNKK ) in ras transformed cells with normally elevated JNK activity switches the protease dependent invasive phenotype from a urokinase plasminogen activator ( uPA ) dependent to a cathepsin L ( CL ) dependent invasive phenotype . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Cysteine proteases [ Cathepsin B and L ( CATB , CATL ) ] and the serine protease urokinase type plasminogen activator ( UPA ) with its inhibitor type 1 ( PAI 1 ) are thought to play an important part in colorectal cancer invasion and metastasis . ^^^ Significantly higher antigen levels were found : 1 . in cancerous tissue vs . tumour free tissue ( CATB , CATL , UPA , PAI 1 ) ; in colorectal cancer with vs . without metastasis ( CATB , CATL , UPA , PAI 1 ) ; 3 . in poorly vs . well differentiated tumours ( CATB , UPA , PAI 1 ) ; 4 . in advanced Dukes ' stages ( CATB , UPA , PAI 1 ) . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Cathepsin B ( CATB ) and cathepsin L ( CATL ) , which are cysteine proteases , urokinase ( UPA ) and tissue type plasminogen activator ( TPA ) , both serine proteases , and their inhibitor type 1 ( PAI 1 ) are believed to play an important role in colorectal carcinoma ( CRC ) invasion and metastasis . ^^^ The objective of this study was to measure CATB , CATL , UPA , TPA , and PAI 1 in the same cancerous tissue ( CANCER ) and in tissues obtained from a tumor free area ( NORMAL ) to compare their respective prognostic roles in patients with CRC . ^^^ RESULTS : Significantly higher antigen levels were found : 1 ) in CANCER versus NORMAL ( with respect to CATL , UPA , and PAI 1 , with significantly lower levels for TPA ) ; 2 ) in CRC with versus without metastasis ( CATB , CATL , and PAI 1 ) ; 3 ) in poorly versus well differentiated CRC ( UPA and PAI 1 ) ; and 4 ) in advanced Dukes stages ( PAI 1 ) . ^^^ CATB and CATL significantly correlated with UPA and PAI 1 . ^^^ Finally , CATL ( P = 0 . 0001 ) , UPA ( P = 0 . 006 ) , PAI 1 ( P = 0 . 006 ) , Dukes stage ( P = 0 . 0001 ) , presence of metastases ( P = 0 . 003 ) , and vascular invasion ( P = 0 . 03 ) had a significant prognostic impact . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Our purpose was to analyze the predictive relevance of DNA ploidy , S phase fraction ( SPF ) , and tissue levels of lysosomal proteinases cathepsin D ( CD ) , cathepsin B ( CB ) , cathepsin L ( CL ) , and urokinase type plasminogen activator ( uPA ) and that of the intracellular cysteine proteinase inhibitor stefin A on clinical outcome . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| A study was performed to correlate the expression of junctional complex components ( such as zonula occludens 1 [ ZO 1 ] , a tight junction component protein ) and nonjunctional complex components ( such as urokinase type plasminogen activator [ uPA ] , a serine protease ; cathepsin L , a cysteine protease ; alpha 2 macroglobulin , a nonspecific protease inhibitor ; and cystatin C , a cysteine protease inhibitor ) at the time when inter Sertoli tight junctions were established in vitro . ^^^ More interestingly , there was also a transient increase in the expression of uPA and cathepsin L between days 2 and 3 at the time preceding the formation of tight junctions . ^^^ In Sertoli cells cultured at low density ( 2 10 10 ( 4 ) cells / cm2 ) , when a confluent monolayer of cells could not form , there were no changes in the expression of either ZO 1 , uPA , or cathepsin L throughout the 7 day culture period . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Human T blasts and SupT 1 lymphoma cells expressed mRNA of MMP 9 , MT 1 MMP , MT 4 MMP , cathepsin L , uPA , and uPAR as well as ADAM 9 , 10 , 11 , 15 , and 17 . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Other factors examined ( including the laminin receptor , cathepsin B , cathepsin L , urokinase type plasminogen activator , and tissue inhibitor metalloproteinases ) are expressed by both invasive and noninvasive gastric cancer cells , whereas collagenase type 4 92 kD form is not expressed by any of the cells examined . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| The transformed cells also showed a small increase in the activity of gelatin degrading matrix metalloproteinases ( MMPs ) and urokinase type plasminogen activator activities , neither of which was sensitive to the inhibitors of cathepsin L and B . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| At present , antithrombotics , the urokinase type plasminogen activator system , the membrane bound membrane type 1 MMP , cathepsin L and the proteasome seem the most promising candidates as targets for anticancer strategies in early stages of cancer in combination with cytotoxic drugs . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| The results implied cathepsin L is a predominant proteinase responsible for autolysis of ATF muscle at elevated temperatures . . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Some of these genes were already known to be associated with apoptosis ( BIK , TEGT , SSI 3 ) , hypoxia inducible genes ( HIF1A , CA 12 ) , and tumor cell invasion and metastasis ( CTSL , CTSB , PLAU , CD 44 ) . ^^^ |
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| Interacting proteins: P00749 and P07711 |
Pubmed |
SVM Score :0.0 |
| Enzyme linked immunosorbent assays ( ELISAs ) were used to measure cysteine cathepsin B ( CatB ) and cathepsin L ( CatL ) and their inhibitors , stefin A ( StA ) and stefin B ( StB ) , together with urokinase ( u PA ) and plasminogen activator inhibitor 1 ( PAI 1 ) , in 150 cytosols of primary invasive breast carcinoma . ^^^ In the total group of patients , high u PA and PAI 1 and low StB levels correlated significantly with shorter disease free survival ( DFS ) , while CatB , CatL and StA did not . ^^^ In lymph node negative patients , high CatB and CatL were also associated with shorter DFS , while u PA remained the most significant of all these biological markers . ^^^ In conclusion , this retrospective study showed u PA to be of better prognostic relevance than the cysteine proteinases , though CatB and CatL were relevant for prognosis in lymph node negative breast cancer patients . . ^^^ |
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