| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The specific increase in platelet GPIV in patients with essential thrombocythemia was confirmed by immunoblot analysis whereas no increase in platelet GPIb or GPIIb IIIa was observed by this technique . . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Gold particles identifying glycoprotein ( GP ) Ib ( CD42b ) and GPIIb IIIa ( CD41a ) remained distributed over the entire platelet surface , whereas gold particles identifying GPIa IIa ( CDw 49b ) and GPIV ( CD 36 ) were found essentially overlying the granulomere ; p 24 ( CD 9 ) was present at the peripheral platelet rim and over the cell body . ^^^ An increased labeling of GPIIb IIIa , GPIV and p 24 was also observed on pseudopods , with GPIIb IIIa and GPIV concentrated at the enlarged extremities and at sites of contact between two platelets , whereas GPIb was absent from pseudopods . ^^^ Double labeling experiments showed colocalization of Fg and TSP , GPIV and TSP , as well as Fg and GPIIb IIIa , although no typical coclustering of GPIIb IIIa and GPIV or GPIIb IIIa and p 24 was apparent . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Removal of the Mr 45 , 000 amino terminal part of GPIb alpha by treatment with elastase did not abrogate association of GPV with GPIb 9 , showing that the leucine rich repeat sequences in GPIb alpha are not required for complex formation . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Adherence was unaffected by monospecific polyclonal antibodies to glycoprotein ( GP ) Ib and GPIV , and was normal with platelets from two patients with Glanzmann ' s thrombasthaenia , indicating that GPIb , the GPIIb / IIIa complex and GPIV are not involved in platelet laminin interaction . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| In addition , autoradiography of the gels showed a 125I 105 Kd GP , identified by immunoblot as GPIIIa , as well as GPIb , GPIIb , and another band at 87 Kd , probably GPIV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The up regulation of von Willebrand factor receptor ( GPIb ) and thrombospondin receptor ( GPIV ) and the down regulation of fibrinogen receptor ( GPIIb / IIIa ) and C3bi receptor ( CD11b ) were found by incubation with MAbs AN 51 ( CD42b ) , OKM 5 ( CD 36 ) , J 15 ( CD41a ) , and OKM 1 ( CD11b ) , respectively . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| In contrast , in the presence of 2 mM Ca2+ and 1 mM Mg2+ , 5G11 precipitated a complex of five radiolabeled proteins , among which GPIIb , GPIIIa and GPIV were the most prominent . . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| On one dimensional analysis , Bernard Soulier ' s syndrome ( BSS ) platelets also lacked the peripheral membrane glycoprotein , GPV ( mol wt 82 , 000 ) and a low molecular weight glycoprotein , GPIX , ( nonreduced or reduced , mol wt congruent to 22 , 000 ) . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Other mAb , such as those against the von Willebrand factor receptor ( GPIb ) ( CD42b ) , the thrombospondin receptor ( GPIV ) ( CD 36 ) , and GPIIIa ( CD 61 ) , also showed statistically significant reduction of mAb binding but to a lesser degree . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| MoAbs against GPIa GPIb alpha , GPV , P selectin , and the alpha chain of the vitronectin receptor did not react with CD34+ cells . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Using flow cytometry , we found transfected GPV was surface expressed in HEL cells ; this , in turn , led to increased surface expression of the ligand binding GPIb alpha and GPIX subunits . ^^^ We employed confocal microscopy of GPV transfected HEL cells to show that GPV colocalizes with GPIb alpha on the cell surface . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The complex is composed of four glycoprotein subunits , GPIb alpha , GPIb beta , GPIX and GPV , each with a variable number of leucine rich repeats . ^^^ GPIb alpha and GPIb beta are linked by a disulphide bridge while GPIX and GPV associate noncovalently with the complex . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Western blotting revealed normal levels of GPIX , decreased levels of GPIb beta and GPV , and < 1 % of GPIb alpha . ^^^ RT PCR studies showed the presence of mRNA coding for GPIb alpha , GPIb beta , GPIX and GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Like the genes encoding GPIb alpha , GPIX , and GPV , the GPIb beta gene appears simple in structure . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The patients ' platelets had 46 % , 46 % , and 24 % of control levels of GPIb alpha and 43 % , trace , and 13 % of control levels of GPV as determined by immunoblot analysis . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| A panel of fluorescent labeled monoclonal antibodies was used to monitor activation dependent platelet surface changes : downregulation of glycoprotein ( GP ) Ib ( 6D1 ) and upregulation of GMP 140 ( S 12 ) , the GPIIb IIIa complex ( PAC 1 ) , and GPIV ( OKM 5 ) . ^^^ These changes were more readily detected with monoclonal antibodies directed against GPIb ( 6D1 ) and , to a lesser extent , GPIV ( OKM 5 ) rather than those directed against the GPIIb IIIa complex ( PAC 1 ) and GMP 140 ( S 12 ) . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Cathepsin G proteolysed platelet surface GPIb alpha , but redistributed platelet surface GPIX and GPV to the SCCS . ^^^ Elastase proteolysed platelet surface GPIb alpha and GPV , but , unlike the other agonists tested , neither proteolysed nor redistributed platelet surface GPIX . ^^^ First , although only the GPIb alpha subunit of this putative complex is known to be directly linked to the platelet cytoskeleton via actin binding protein , cytochalasin B inhibited the ADP / epinephrine , cathepsin G , and TRAP induced decrease in platelet surface GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet membrane glycoprotein ( Gp ) Ib complex consists of four polypeptides : the disulfide linked GpIb alpha and GpIb beta subunits ; GpIX , tightly , but noncovalently associated with GpIb alpha beta ; and the more weakly associated GpV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Membrane glycoproteins : GPIb ( CD42b ) , GPIIb IIIa ( CD41a ) , GPIV ( CD 36 ) ; and activation dependent antigens : P selectin ( CD62P ) and lysosomal glycoprortein ( CD 63 ) , were detected in whole blood by dual color flow cytometry . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| In case 1 , GPIX was completely absent but residual amounts of GPIb alpha and GPV were detectable ; in case 2 , GPIb alpha was completely absent . ^^^ We amplified the coding regions of GPIb alpha , GPIb beta , GPV , and GPIX from the patients ' genomic DNA with the polymerase chain reaction ( PCR ) and sequenced the PCR products . in case 1 , we identified a point mutation in the GPIX coding region that changes the codon for tryptophan 126 ( TGG ) to a nonsense codon ( TGA ) . ^^^ Thus , abnormality of a single component protein ( GPIX or GPIb alpha ) alters the assembly of the GPIb / IX / V complex and causes heterogeneous surface expression of GPIb alpha , GPV and GPIX . . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Bernard Soulier syndrome ( BSS ) platelets deficient in GPIb 9 and GPV did not react with the sera from our varicella infected study patients . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Flow cytometric analysis showed that GPIX was completely absent but residual amounts of GPIb alpha and GPV were detectable in these patients . ^^^ We analyzed all coding regions of GPIb alpha , GPIb beta , GPV and GPIX which were amplified from the patients ' genomic DNA by the polymerase chain reaction ( PCR ) . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| It is composed of GPIb alpha , GPIb beta , GPIX , and GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet membrane glycoprotein ( GP ) Ib 5 9 complex is the receptor for von Willebrand factor and is composed of four membrane spanning polypeptides : GPIb alpha , GPIb beta , GPIX , and GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet glycoprotein Ib ( GpIb ) complex is composed of four polypeptides : the disulfide linked GpIb alpha and GpIb beta and the noncovalently associated GpIX and GpV . ^^^ Furthermore , using two color fluorescence , we have shown that all ECs expressing GpIb alpha also express GpIX and GpV on their surface . ^^^ The ratio of GpIb alpha : GpIX : GpV is 1 : 1 : 0 . 5 , which is identical to the ratio present in platelets . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Modifications in the presence of platelet glycoproteins GPIb ( CD42b ) , GPIIIa ( CD 41 ) and GPIV ( CD 36 ) , expression of specific platelet markers ( P selectin ( CD62P ) and lysosomal protein ( CD 63 ) ) and leukocyte integrin ( CD11b ) were assessed during hemodialysis . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Glycoprotein Ibalpha contains a binding site for von Willebrand factor through which it mediates platelet adhesion ; GP 5 is required for the complex to bind thrombin with high affinity ; and both GP Ibbeta and GP 9 are necessary for efficient plasma membrane expression of the complex . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelets from patients with TTP did , however , have significantly reduced levels of GPIIb / IIIa prior to plasmapheresis ( mean 36 348 5 52 505 molecules / platelet in controls ; P = 0 . 0004 ) and of GPIV ( mean 13 321 5 26 212 molecules / platelet in controls ; P = 0 . 0002 ) . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Whether GPV deficiency might also cause BSS is unknown as are the roles of GPV in platelet vWF interaction and thrombin signaling . ^^^ Our results show that ( 1 ) GPV is not necessary for GPIb expression and function in platelets and that GPV deficiency is not likely to be a cause of human BSS and ( 2 ) GPV is not necessary for robust thrombin signaling . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| All these 15 patients had antibodies against GPIIb / IIIa , five patients against GPIb / IX , and six patients against GPIV . ^^^ Anti GPIb / IX and GPIV occurred only in combination with anti GPIIb / IIIa antibodies . ^^^ Eluates from platelets contained only GPIIb / IIIa reactivities , but neither anti GPIb / IX nor anti GPIV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Change in platelet surface markers were measured by flow cytometry , using fluorescein conjugated antibodies to fibrinogen , P selectin ( CD62P ) , GPIIb IIIa ( CD 41 ) , GPIb alpha ( CD42b ) and GPV ( CD42d ) , and fluorescein conjugated Annexin 5 was used to measure expression of anionic phospholipid . ^^^ All concentrates showed some changes during preparation but PRP PC underwent the greatest changes with significantly higher levels of P selectin ( P < 0 . 001 ) and bound Annexin 5 ( P=0 . 001 ) than AP PC or BC PC , and lower levels of GPIb alpha ( P=0 . 002 ) and GPV ( P < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The role of GPV , also absent in BSS , was recently addressed by gene targeting in mice . ^^^ Thus GPV is not crucial to MK development and platelet production , consistent with the fact that no mutation in the GPV gene has as yet been described in BSS . . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| We observed a reduced cell surface expression of GPIb ( p < 0 . 01 ) and GPIIb / IIIa ( p < 0 . 01 ) , while GPIa / IIa and GPIV expression was only marginally different between patients and controls . ^^^ In contrast , there was a two fold increase of platelet GPIb and GPIIb RNA and a three fold increase of GPIV RNA among MDS patients . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Analysis of genes encoding for GPIb alpha and beta , GPV , and GPIX ruled out the possibility that the observed platelet abnormality is a variant of Bernard Soulier syndrome . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The glycoprotein Ib ( GPIb ) complex is composed of GPIb alpha covalently attached to GPIb beta and noncovalently complexed with GPIX and GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Tests with GPV transfectants revealed that the antibodies reacted with GPV independent of GPIb alpha , GPIb beta , or GPIX . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The complex consists of four polypeptides GPIb alpha , GPIb beta , GPIX and GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Flow cytometric analysis showed that TMVA did not bind to platelet GPIX , GPIIb , GPIIIa , GPIa , GPIIa and GPIV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| After 48 h of shear stress , cell surface GpIb alpha , GpIX , and GpV , analyzed by flow cytometric analysis , were further increased over the levels observed at 24 h . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| The complex , which serves as a platelet receptor for von Willebrand factor , is composed of 4 subunits : GPIb alpha , GPIb beta , GPIX , and GPV . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Bernard Soulier Syndrome ( BSS ) is a severe congenital platelet disorder that results from a deficiency of the platelet membrane glycoprotein ( GP ) Ib / IX complex that is composed of four subunits ( GPIbalpha , GPIbbeta , GPIX , and GPV ) . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Several mutations have been described for each of the subunits , except for GPV , as a cause of BSS . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet glycoproteins Ib beta ( CD42c ) , 9 ( CD42a ) , and 5 ( CD42d ) , together with GP Ib alpha ( CD42b ) , form a receptor whose interaction with the von Willebrand factor is essential in the initial stages of haemostasis . ^^^ |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40197 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|