| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Some particles also expressed the platelet specific glycoproteins GPIIb , IIIa and GMP 140 . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| P selectin glycoprotein ligand ( PSGL 1 ) shares common features with platelet glycoprotein Ibalpha . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Role of platelet surface glycoprotein Ibalpha and P selectin in the clearance of transfused platelet concentrates . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Binding of bone marrow cells to platelets involves both P selectin and GPIIb integrin on platelets . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Paraformaldehyde fixed platelets were incubated with 2 murine monoclonal antibodies : CD42b and CD 62 . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| For flow cytometric analysis monoclonal antibodies CD41a , CD42b , CD62p and CD 63 were applied . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| The following conjugated MoAb were used : anti CD41a , CD 36 , CD42b , CD62P , CD 63 , factor V / Va and nonspecific Ig . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| CD42b ) or activation dependent antigens ( CD62p , CD 63 , LIBS , RIBS ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Flow cytometry was used to measure the expression of glycoprotein 1b ( GP1b ) and alpha granule membrane protein 140 ( GMP 140 ) on platelets . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| A panel of fluorescent labeled monoclonal antibodies was used to monitor activation dependent platelet surface changes : downregulation of glycoprotein ( GP ) Ib ( 6D1 ) and upregulation of GMP 140 ( S 12 ) , the GPIIb IIIa complex ( PAC 1 ) , and GPIV ( OKM 5 ) . ^^^ These changes were more readily detected with monoclonal antibodies directed against GPIb ( 6D1 ) and , to a lesser extent , GPIV ( OKM 5 ) rather than those directed against the GPIIb IIIa complex ( PAC 1 ) and GMP 140 ( S 12 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| The estimation of platelet adhesion and activation was performed with two enzyme immunoassays ( EIAs ) using monoclonal antibodies directed against CD42b ( GP lb ) and CD 62 ( GMP 140 or P Selectin ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| RESULTS : When multiple samples from individual patients were assessed , the degree of activation with all of the activation assays ( GMP 140 , thrombospondin , activated GPIIb / IIIa , FXIIIa ) was significantly greater in samples taken from the arterial catheter ( p < 0 . 05 ) compared with the central venous catheter or the peripheral vein . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Infusion of 1 desamino 8 D arginine vasopressin normalized bleeding time in the two severely affected patients , although it did not modify ristocetin induced platelet agglutination or membrane expression of GPIbalpha , GPIX , GPIIb IIIa and GMP 140 . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| In five patients from each group , platelet receptors for glycoprotein IIb IIIa ( GPIIb IIIa ) and expression of platelet activation markers , guanosine monophosphate 140 ( GMP 140 ) and lysosomal protein , were measured by flow cytometry before and after CPB . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| We analyzed the fluorescence expression of structural antigens CD41a ( GPII IIIa ) and CD42b ( GPIb 5 9 ) , and of the activation dependent antigens CD62p ( P selectin , PADGEM , GMP 140 ) and CD 63 ( GP 53 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| To test this hypothesis , we studied the in vitro effects of aCL and LA on the enhancement of platelet activation by flow cytometric analysis using anti CD62P and anti CD 41 monoclonal antibodies directed against platelet activation dependent granule external membrane ( PADGEM ) protein and platelet glycoprotein IIb ( GPIIb ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| To test this possibility , we studied the in vitro effects of aCL and LA on the enhancement of platelet activation by flow cytometric analysis using anti CD62P and anti CD 41 monoclonal antibodies directed against platelet activation dependent granule external membrane ( PADGEM ) protein and platelet glycoprotein IIb ( GPIIb ) , respectively . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Activated platelets were detected by using a panel of fluoresceinated mAbs specific for activation dependent platelet epitopes , including 1 ) activated GPIIb IIIa complex ( PAC 1 ) ; 2 ) fibrinogen bound to platelet GPIIb IIIa ( 9F9 ) ; 3 ) ligand induced binding sites on GPIIIa ( anti LIBS 1 ) ; and 4 ) P selectin , an alpha granule membrane protein expressed on the platelet surface after secretion ( S 12 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| MoAbs against GPIa GPIb alpha , GPV , P selectin , and the alpha chain of the vitronectin receptor did not react with CD34+ cells . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Guinea pig specific probes for P selectin , von Willebrand factor ( vWF ) , glycoprotein Ib alpha ( GpIb alpha ) , and phosphoglycerate kinase were prepared by using the polymerase chain reaction . ^^^ By Northern blot analysis , P selectin messenger ribonucleic acid ( mRNA ) was primarily expressed in the mature megakaryocyte Celsep subgroup , whereas vWF and GpIb alpha mRNA were expressed at all phases of megakaryocyte maturation . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Membrane glycoprotein expression was assessed by flow cytometry with fluorescein isothiocyanate labeled monoclonal antibodies ( MoAbs ) directed against the following antigens : GPIb ( CD42b ) , CD 63 ( an activation protein ) , P selectin ( CD 62 ) , and GPIIb / IIIa ( CD41a ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Fixed platelets , stained with monoclonal fluorescein ( FITC ) labeled antibodies directed against GPIb ( CD42b antigen ) , GPIb / IX , GPIIb / IIIa ( CD41a antigen ) , CD 63 antigen ( a platelet activation protein ) , and P selectin ( CD 62 antigen ) were studied by flow cytometry . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Membrane glycoproteins : GPIb ( CD42b ) , GPIIb IIIa ( CD41a ) , GPIV ( CD 36 ) ; and activation dependent antigens : P selectin ( CD62P ) and lysosomal glycoprortein ( CD 63 ) , were detected in whole blood by dual color flow cytometry . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Analysis was performed with two color flow cytometry using fluoresceine isothiocyanate ( FITC ) and phycoerythrin ( PE ) conjugated monoclonal antibodies ( MoAbs ) directed against GPIb CD42b ) , GP IIb IIIa ( CD 41 ) , P selectin ( CD62P ) , and MoAb PAC 1 directed against activated GP IIb IIIa . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| METHODS : Expression of seven platelet surface antigens ( including P selectin , activated GPIIb , IIIa and GPIb 9 ) , whole blood platelet aggregation , platelet count and hematocrit were measured before and after arising in 17 normal volunteers . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Receptor expression was measured by flow cytometry with monoclonal murine anti human antibodies CD 9 ( p 24 ) , CD42B ( Ib ) , CD41b ( IIb ) , CD 61 ( IIIa ) , CD41a ( IIb / IIIa ) , CD49b ( VLA 2 ) , CD62p ( P selectin ) , CD 31 ( PECAM 1 ) , and CD51 / CD61 ( vitronectin ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Change in platelet surface markers were measured by flow cytometry , using fluorescein conjugated antibodies to fibrinogen , P selectin ( CD62P ) , GPIIb IIIa ( CD 41 ) , GPIb alpha ( CD42b ) and GPV ( CD42d ) , and fluorescein conjugated Annexin 5 was used to measure expression of anionic phospholipid . ^^^ All concentrates showed some changes during preparation but PRP PC underwent the greatest changes with significantly higher levels of P selectin ( P < 0 . 001 ) and bound Annexin 5 ( P=0 . 001 ) than AP PC or BC PC , and lower levels of GPIb alpha ( P=0 . 002 ) and GPV ( P < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Ppep induced aggregation is also associated with indices of platelet activation including thromboxane A 2 ( TXA 2 ) synthesis ( EC 50 = 45 + / 5 microM ) , secretion of alpha granules detected as enhanced surface expression of P selectin ( EC 50 = 52 + / 8 microM ) , and conformational changes in GpIIb / IIIa measured by the monoclonal antibody , PAC 1 ( EC 50 = 3 . 7 + / 1 microM ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Receptor expression was measured by flow cytometry with monoclonal antibodies to p 24 ( CD 9 ) , Ib ( CD42b ) , IIb ( CD41b ) , IIIa ( CD 61 ) , IIb / IIIa ( CD41b / CD61 ) , very late antigen 2 ( CD49b ) , P selectin ( CD62p ) , platelet / endothelial cell adhesion molecule 1 ( CD 31 ) ; and vitronectin ( CD51 / CD61 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Modifications in the presence of platelet glycoproteins GPIb ( CD42b ) , GPIIIa ( CD 41 ) and GPIV ( CD 36 ) , expression of specific platelet markers ( P selectin ( CD62P ) and lysosomal protein ( CD 63 ) ) and leukocyte integrin ( CD11b ) were assessed during hemodialysis . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Higher expression of P selectin ( 1 . 4 fold , NS ) and the reduction in GPIb alpha expression ( by 27 . 8+ / 16 . 7 % , p < 0 . 0002 ) , as well as the increased fractions of platelet microparticles ( p < 0 . 03 ) , reflected more intensified platelet release reaction in diabetic platelets . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Modifications in platelet activation markers ( P selectin [ CD62P ] ) and lysosomal related protein ( UMPS [ CD 63 ] ) , presence of membrane glycoproteins ( GPIb [ CD42b ] , GPIIb IIIa [ CD41a ] , GDIV [ CD 36 ] , binding of von Willebrand factor ( vWF ) , fibrinogen ( Fg ) and factor 5 [ FV ] ) were analyzed in normal donors ( n = 10 ) and in a severe von Willebrand patient ( type 3 ) von Willebrand disease [ vWD ] ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| We revealed that the presence of 10 mg / ml procaine significantly hampered platelet release reaction , as demonstrated by the significant reduction in the expression of platelet P selectin ( CD 62 ) on one hand , and significantly enhanced expression of GPIb alpha ( CD42b ) antigen on the other , following either 1 hour incubation of washed platelets at room temperature ( % CD 62 : 37 . 1+ / 6 . 8 % of control incubated without procaine , p < < 0 . 0001 ; % CD42b : 116 . 2+ / 6 . 3 % of control , p < 0 . 0001 ) or activation of whole blood platelets with ADP , TRAP , or thrombin . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Levels of Mpl , GpIIb , or P selectin were virtually identical in platelet lysates obtained from normal , TPO knockout and mildly TPO stimulated mice . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| For flow cytometric analysis monoclonal antibodies to CD41a ( glycoprotein IIb / IIIa ) , CD42b ( glycoprotein Ib 5 9 ) , CD62p ( P selectin ) , and CD 63 ( glycoprotein 53 ) were used . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| STUDY DESIGN AND METHODS : In this study , fresh , 3 to 4 day old liquid preserved , and cryopreserved human platelets were studied by the use of monoclonal antibodies directed against p selectin , glycoprotein ( GP ) Ib , activated GPIIb / IIIa , and coagulation factor 5 in a three color flow cytometric method . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet activation was measured on days 1 , 3 , 7 and 9 by flow cytometry using fluoresceinisothiocyanate labeled monoclonal antibodies ( mAbs ) against glycoprotein IIb / IIIa ( CD41a , PAC 1 and LIBS 1 ) , P selectin ( CD62P ) or CD 40 ligand receptor ( CD40L ) in combination with a phycoerythrin labeled panspecific platelet marker against GPIb ( CD42b ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| We have also demonstrated that shear induced surface expression of P selectin as well as microparticle release from platelets depended at least on the interaction between von Willebrand factor and glycoprotein Ibalpha , a platelet surface receptor for the former . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| And isolated reports suggest other platelet polymorphisms ( GPIIb , FcgammaRIIa , P selectin , alpha 2 adrenergic receptor , transforming growth factor [ TGF ] beta ) are risk factors for arterial disease or produce a prothrombotic phenotype . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| The expression of platelet receptors was determined by using the following monoclonal antibodies : CD 31 ( PECAM 1 ) , CD 41 ( GPIIb ) , CD42b ( GPIb ) , CD51 / CD61 ( vitronectin receptor ) , CD62p ( P selectin ) , CD 63 ( LIMP or LAMP 3 ) , CD107a ( LAMP 1 ) , CD 151 ( PETA 3 ) , and PAC 1 for fibrinogen platelet binding determination ( PharMingen , San Diego , CA ) . ^^^ These differences reached statistical significance ( p < 0 . 05 ) for PECAM 1 , GPIIb , and P selectin expression . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| In conclusion , hypercholesterolemia primes platelets for recruitment via VWF , GPIb alpha , and P selectin to lesion prone sites , before lesions are detectable . . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Using flow cytometry we measured the percentage of platelet microparticles , platelet platelet aggregates , and surface expression of CD62P ( P selectin ) and CD42b ( a component of von Willebrand factor receptor ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Samples were stained with fluorescent antibodies against markers of platelet activation ( P selectin [ CD 62 ] , lysosomal protein [ CD 63 ] , and fibrinogen and von Willebrand factor receptors [ CD 41 and CD42b , respectively ] ) and analyzed by flow cytometry . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet serotonin release , and the surface expression of platelet receptors with or without apyrase were determined using the following monoclonal antibodies : anit CD 41 [ glycoprotein ( GP ) IIb / IIIa ] , CD42b ( GPIb ) , CD62p ( P selectin ) , CD51 / CD61 ( vitronectin receptor ) , CD 31 [ platelet / endothelial cellular adhesion molecule 1 ( PECAM 1 ) ] , CD107a [ lysosomal associated membrane protein ( LAMP ) 1 ] , CD107b ( LAMP 2 ) , CD 63 ( LIMP or LAMP 3 ) , and CD 151 ( PETA 3 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : Abciximab ( 50 microg / mL ) reduced the mass of platelets attached to monocytes , measured by the mean fluorescence intensity ( MFI ) of CD42b on CD14+ cells , 1 ( CD42b , 471+ / 197 versus 1073+ / 217 MFI , mean+ / SD , P < 0 . 05 ) , 5 , and 10 minutes after thrombin receptor activator stimulation of whole blood to the same extent as anti P selectin ( 50 microg / mL ; 288+ / 177 MFI , P < 0 . 05 ) when determined by flow cytometry . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Using a direct immunofluorescence labelling technique and whole blood flow cytometry , the effect of PFA fixation was investigated for 4 different epitopes on platelet surface each of which mirrors a different aspect of platelet activation , namely P selectin ( CD62P ) , GP IIbIIa complex ( CD 41 ) , the fibrinogen binding site of the activated GP IIaIIIb complex ( PAC 1 ) and GP Ib 5 9 complex ( CD42b ) . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| STUDY DESIGN AND METHODS : In both human and baboon fresh and lyophilized PLTs , aggregation response and PLT production of thromboxane A 2 were measured after stimulation , and PLT surface markers P selectin , glycoprotein ( GP ) Ib , GPIIb IIIa , and factor ( F ) 5 were measured before and after stimulation . ^^^ RESULTS : Fresh PLTs responded to the dual agonists arachidonic acid and adenosine diphosphate ( ADP ) to aggregate and produce thromboxane A 2 , and in both the PLT surface markers P selectin and GPIIb IIIa increased and GPIb decreased after stimulation . ^^^ Neither human nor baboon lyophilized reconstituted PLTs aggregated to dual agonists , and neither produced thromboxane A 2 , increased PLT surface markers P selectin or GPIIb IIIa , or decreased PLT GPIb after stimulation . ^^^ CONCLUSIONS : Lyophilized reconstituted PLTs exhibited modification of the PLT membrane that interfered with aggregation and thromboxane production , prevented increases in PLT P selectin and GPIIb IIIa and decreases in GPIb after stimulation , and increased FV accumulation after recalcification . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Adhesion molecules , receptor expression ( CD42a , CD42b , CD 36 , CD 29 , PAR 1 ) , and alpha granule secretion detected by P selectin expression remained unchanged but the release of growth factors was differentially regulated with increased VEGF and unchanged PDGF after agonist activation even in uncomplicated sepsis . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| For example , microparticles in these experiments were found to contain membrane surface proteins including GPIIIa , GPIIb , and P selectin , as well other platelet proteins such as the chemokines CXCL 4 , CXCL 7 , and CCL 5 . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Despite the difference in the coagulation factors binding , the subpopulations were indistinguishable by the expression of general platelet marker CD42b and activation markers PAC 1 ( an epitope of glycoprotein IIb / IIIa ) and CD62P ( P selectin ) . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Independently of dose and duration , valsartan provided early sustained significant inhibition of adenosine diphosphate induced platelet aggregation , decreased shear induced activation measured with PFA 100 analyzer , and diminished expression of GP IIb / IIIa activity measured by PAC 1 antibody , GPIb ( CD42b ) , vitronectin receptor ( CD51 / 61 ) , P selectin ( CD62p ) , lysosome associated membrane protein ( CD107a ) , and CD 40 ligand ( CD 154 ) . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet P selectin , which increased significantly after activation , triggered platelet binding to neutrophils that was completely inhibited by P selectin or PSGL 1 antagonists , and was reduced by 50 % with a GPIIb / IIIa antagonist . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| We examined the expression of adhesion molecules of the integrin family ( CDw49b , CDw49d , CDw49e , CDw49f , CD 18 , CD11a CD11c , and CD11b ) , the immunoglobulin superfamily ( CD 54 , CD 56 , CD 58 , and CD 31 ) , the selectin family ( ELAM 1 , LECAM 1 , and CD 62 ) , and CD 44 , CD41b , and CD42b on platelets , megakaryocytes , and megakaryocytes with CP . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet surface glycoproteins were examined by flow cytometry after platelets were fixed in dilute plasma with 1 percent formaldehyde and stained with fluorescein isothiocyanate labeled monoclonal antibodies CD42b ( anti glycoprotein [ GP ] lb ) , CD41a ( anti GPllb / llla ) , and CD 62 ( anti P selectin ) . ^^^ RESULTS : The binding of anti CD42b was greater in plateletpheresis concentrates than in random donor platelets on Days 3 and 5 ( p < 0 . 01 ) of storage ; binding of anti CD 62 was greater in the random donor concentrates ( p < 0 . 01 ) on Days 3 and 5 . ^^^ CONCLUSION : Platelet concentrates prepared from single units of whole blood and anticoagulated with CPDA 1 bind less anti CD42b and more anti CD 62 than do platelets obtained by apheresis . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| In addition , membrane glycoproteins in platelets obtained at these time intervals were studied using fluorescein isothiocyanate ( FITC ) conjugated monoclonal antibodies ( mAb ) CD42b ( anti GPIb ) , CD41a ( anti GPIIb / IIIa ) , and CD 62 ( anti P selectin ) , with flow cytometry . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| We found that addition of Hb raffimer to blood samples up to 50 vol % did not affect human platelets as measured by various markers of platelet activation ( CD42b , CD 41 , PAC 1 , CD 62 , CD 63 ) , procoagulant activity ( annexin 5 ) and microparticle formation ; differences between Hb raffimer and lactated Ringer ' s diluted blood were not significant . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet surface markers CD41a , CD42b , CD62P , and CD 63 were analyzed by flow cytometry , and the antibody binding sites were quantified by using microbeads . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| At fixed intervals over a 10 min time course , aliquots of PRP were drawn , stained with monoclonal antibodies ( CD41a , CD42b , CD62p , and CD 63 ) , and analyzed by flow cytometry . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Expression of structural antigens CD41a and CD42b and activation dependent antigens CD62p , CD 63 , and fibrinogen was analyzed by flow cytometry at fixed time intervals . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet surface expression of glycoproteins CD41a , CD42b , CD62p , and CD 63 was analyzed by flow cytometry . ^^^ CONCLUSION : The varying kinetics of expression , as measured by the MCFI , of platelet antigens CD62p , CD 63 , CD41a , and CD42b during extracorporeal circulation may be useful for biocompatibility testing . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet and leukocyte activation and percentage of leukocyte platelet aggregates were determined in a whole blood assay by subsequent staining for activation associated antigens ( CD41a , CD42b , CD62p , and fibrinogen binding ) and leukocyte antigens ( CD 14 and CD 45 ) and flow cytometric analysis . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet surface expression of the CD62P ( reflecting alpha granule release ) , CD 63 ( reflecting lysosomal release ) and modulation of normal platelet membrane glycoproteins CD41a and CD42b , were measured in whole blood and in isolated platelets immediately after collection and at 6 , 24 and 48 h after venipuncture . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| During storage , the mean channel fluorescence intensity of CD41a and CD42b , the percentage of CD62p and CD 63 positive platelets , and the binding of fibrinogen to platelets showed no significant change . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Daily aliquots of the platelet rich plasma were obtained to measure Annexin 5 and platelet antigens CD62p , CD 63 , CD41a , CD42b , and the binding of fibrinogen . ^^^ During storage , no significant changes in mean channel fluorescence intensity ( MCFI ) of CD41a ( P = 0 . 99 ) and CD42b ( P = 0 . 29 ) , percentage of CD62p+ and CD63+ platelets ( P = 0 . 23 for CD62p ; P = 0 . 52 for CD 63 ) , and the binding of fibrinogen to platelets occurred ( P = 0 . 85 ) . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| CD42b , CD 61 , and CD62p expression were significantly increased in 11 patients compared with control subjects ( CD42b p < 0 . 001 , CD 61 p < 0 . 005 and CD62p p < 0 . 001 ) . ^^^ In addition , after ticlopidine treatment , platelets showed a significant fall in expression of all these markers in 11 patients ( CD42b p < 0 . 001 , CD 61 p < 0 . 005 and CD62p p < 0 . 001 ) . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Neither monoclonal APA had an effect on platelets as determined by flow cytometric analysis of CD62P , CD 41 , CD42b expression and fibrinogen binding with and without previous activation with 5 microM ADP or 15 microM TRAP 6 . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Selectins ( CD62L , CD62P ) and megakaryocytic glycoproteins ( CD41a , CD42b ) mediate megakaryocyte fibroblast interactions in human bone marrow . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Three surface glycoprotein CAMs , P selectin ( CD62P ) , GPIba in the GPI 9 complex ( CD42a / CD42b alpha , b beta ) , and the integrin GPIIbIIIa ( CD41 / CD61 ) in the human platelet were selected on the basis of their unique topographic shape . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Following chemotherapy a high surface expression of CD62P , a low mepacrine staining , and a reduced surface expression of CD42b ( part of the GP Ib / V / IX complex ) were found , indicating an irreversible activation of platelets . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| In addition , the expression of platelet receptors was determined by using the following monoclonal antibodies : anti CD 31 , CD 41 , CD42b , CD51 / CD61 , CD62p , CD 63 , CD107a , and CD 151 . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet activation was determined by the expression of CD62P , CD 63 , CD 41 , CD42b , activated GPIIb / IIIa ( PAC 1 ) , procoagulant surface ( as reflected by annexin 5 binding ) , and microparticle formation . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Conventional platelet aggregation induced by 5 microM ADP and 5 microM epinephrine , cartridge based analyzers ( Ultegra , and PFA 100 ) readings , and expression of CD 31 , CD41a , CD42b , GPIIb / IIIa activity , CD51 / CD61 , CD62p , CD 63 , CD107a , CD 154 , CD 165 , formation of platelet monocyte aggregates , intact ( SPAN 12 ) , and cleaved ( WEDE 15 ) PAR 1 thrombin receptors by flow cytometry were analyzed . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Flow cytometric analysis of platelet activation markers demonstrated reduced CD62P [ 35 . 6 vs . 118 . 5 10 10 ( 3 ) molecules of equivalent soluble fluorochrome ( MESF ) ; P < 0 . 0001 ] , CD 63 ( 11 . 3 vs . 50 . 7 10 10 ( 3 ) MESF ; P < 0 . 0001 ) , and PAC 1 ( 41 . 5 vs . 90 . 5 % ; P = 0 . 0001 ) while reductions in CD42b were abnormal ( 45 . 6 vs . 70 % ; P < 0 . 0001 ) . sP selectin levels were similar in patients and healthy controls ( 0 . 04 vs . 0 . 27 fg / platelet ; P = 0 . 84 ) . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Surface expression of CD 61 , CD62P , and CD42b on resting and thrombin stimulated platelets was analyzed with flow cytometry . ^^^ No differences between groups were found in CD 61 , CD62P , and CD42b surface expression , but markers of the coagulation cascade and fibrinolysis or endothelial injury ( F1+2 prothrombin fragments , tissue plasminogen activator inhibitor 1 ) were elevated in patients treated with CyA compared with children on steroids and healthy controls . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet activation ( CD62p , CD 63 , CD42b ) , as measured by flow cytometry , pH , platelet count , swirling effect and storage time , was determined . ^^^ RESULTS : A good correlation ( r 2 > 0 . 5 ) was observed between CD62p and CD 63 , between CD62p and CD42b , between CD62p or CD 63 and pH and between CD62p or CD 63 and swirling effect . ^^^ |
|
| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet activation markers CD62P , CD42b , CD 63 and PAC 1 were analysed following in vitro activation by thrombin receptor activating peptide . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| METHODS : In 80 patients ( 46 ET and 34 PV ) and 50 control subjects , we conducted a flow cytometric analysis to evaluate the levels of PMN platelet aggregates ( defined as the percentage of CD11b positive PMN coexpressing a platelet specific marker , i . e . , CD42b or CD62P ) and the levels of activated PMN and activated platelets . ^^^ In vitro f MLP stimulation upregulated PMN CD11b expression and simultaneously increased CD11b / CD42b and CD11b / CD62P aggregates , without affecting platelet surface antigens . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| The expression of CD62p gene and HSP 70 gene in them were observed , compared and analyzed with blood biochemical indexes by experimental techniques , and compared to those in healthy subjects as control , for exploring the microcosmic mechanism and evolutive rules of BSS formation at the gene level . ^^^ RESULTS : It was showed , through determination and analysis of the microcosmic indexes closely associated with BSS , that the expression of CD62p gene and HSP 70 gene were abnormal in patients with BSS , they were increased in patients with BBS and significantly higher than those in healthy subjects ( P < 0 . 01 ) . ^^^ CONCLUSION : CD62p gene and HSP 70 gene might be closely associated with BSS . . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| Samples were then incubated with either CD 41 and CD62P or CD42b and CD 45 monoclonal antibodies and analyzed by flow cytometry . ^^^ |
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| Interacting proteins: P16109 and P07359 |
Pubmed |
SVM Score :0.0 |
| We evaluated polymorphisms in 40 genes previously associated with coronary heart disease and found significant ( p < 0 . 05 ) associations with 10 : ACE , APOE , F 7 , FGB , GP1BA , IL1RN , LRP 1 , MTHFR , SELP , and THPO . ^^^ |
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