| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The primary sequences of the three individual glycoprotein ( GP ) chains , GPIb alpha , GPIb beta , and GPIX , comprising the normal platelet GPIb / IX receptor for von Willebrand factor ( vWF ) have recently been determined , opening the possibility for characterization of disorders of this receptor at the molecular level . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Anti PlE 1 antibody bound specifically to both the GPIb complex and glycocalicin . 3H labelled platelet membrane glycoproteins with apparent molecular weights of 130k , 25k , and 21k ( under reduced conditions ) corresponding to GPIb alpha , GPIb beta , and GPIX were immunoprecipitated by anti PlE 1 plasma . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Monobromobimane and N dansylaziridine labeled GPIb beta but not GPIb alpha in intact platelets . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| In contrast to GPIb alpha , which is very rich in O linked oligosaccharides , sugar analysis revealed that GPIb beta and GP 17 seem to have only N linked chains of the lactosamine type . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| This was confirmed by two dimensional nonreduced reduced SDS polyacrylamide gel electrophoresis , a procedure that allows clear separation of the disulfide linked subunits of GPIb , GPIb alpha ( mol wt 145 , 000 ) , and GPIb beta ( mol wt 25 , 000 ) from other membrane glycoproteins . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Bernard Soulier syndrome Kagoshima : Ser 444 > stop mutation of glycoprotein ( GP ) Ib alpha resulting in circulating truncated GPIb alpha and surface expression of GPIb beta and GPIX . ^^^ The platelet membrane glycoprotein ( GP ) Ib / IX complex is composed of three polypeptides , GPIb alpha , GPIb beta , and GPIX , and functions as a platelet receptor for von Willebrand factor . ^^^ Her platelets completely lacked the surface expression of GPIb alpha , but expressed a residual amount of GPIb beta and GPIX . ^^^ Furthermore , the truncated GPIb alpha might be associated with the process of the surface expression of incomplete GPIb / IX complex , GPIb beta and GPIX . . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The complex is composed of four glycoprotein subunits , GPIb alpha , GPIb beta , GPIX and GPV , each with a variable number of leucine rich repeats . ^^^ GPIb alpha and GPIb beta are linked by a disulphide bridge while GPIX and GPV associate noncovalently with the complex . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| CHO and L cell lines that express high levels of GP Ib were used to determine whether GP Ib ( GPIb alpha and GPIb beta ) associated with the cytoskeleton . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Western blotting revealed normal levels of GPIX , decreased levels of GPIb beta and GPV , and < 1 % of GPIb alpha . ^^^ RT PCR studies showed the presence of mRNA coding for GPIb alpha , GPIb beta , GPIX and GPV . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Glycoprotein Ib beta ( GPIb beta ) exists in platelets disulfide linked to glycoprotein Ib alpha ( GPIb alpha ) , a major receptor for von Willebrand factor . ^^^ Both GPIb alpha and GPIb beta are expressed in endothelial cells ( EC ) . ^^^ While the GPIb alpha mRNA and protein appear similar in platelets and EC , EC GPIb beta mRNA is larger than platelet GPIb beta and encodes a larger protein . ^^^ Like the genes encoding GPIb alpha , GPIX , and GPV , the GPIb beta gene appears simple in structure . ^^^ When we examined poly ( A ) + RNA from several human tissues for GPIb beta mRNA expression , we found that GPIb beta mRNA was expressed in a variety of tissues but was most abundant in heart and brain , while GPIb alpha and GPIX mRNA expression was found only in lung and placenta at very low levels . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Surface expression of GpIb alpha depends on the correct intracellular assembly with the GpIb beta and GpIX chains . ^^^ This study indicates that functional expression of the vWF binding domain of GpIb alpha on the surface of mammalian cells can be obtained in the absence of GpIb beta and GpIX . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| We analyzed all coding regions of GPIb alpha , GPIb beta , GPV and GPIX which were amplified from the patients ' genomic DNA by the polymerase chain reaction ( PCR ) . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The anti GPIb alpha antibody coprecipitated GPIb beta and GPIX , although the ratios of these polypeptides to GPIb alpha was greatly decreased compared with the ratio in normal platelets . ^^^ Immunoblot analysis under nonreduced conditions showed that most of the GPIb alpha in the patient ' s platelets was not disulfide linked with GPIb beta . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Moreover , a single case of BSS has been associated with a genetic defect of GPIb beta . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| In contrast to previously reported truncated forms of GPIb alpha , this form contains a portion of the transmembranous domain as well as the juxtamembranous cysteines engaged in a disulphide bond with GPIb beta . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| It is composed of GPIb alpha , GPIb beta , GPIX , and GPV . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet membrane glycoprotein ( Gp ) Ib complex consists of four polypeptides : the disulfide linked GpIb alpha and GpIb beta subunits ; GpIX , tightly , but noncovalently associated with GpIb alpha beta ; and the more weakly associated GpV . ^^^ It is not certain whether the association of GpIX to Gplb alpha beta is via GpIB alpha , GpIb beta , or both subunits , although recently published evidence implicates an interaction with GpIb beta . ^^^ We have investigated the interaction of GpIX with GpIb alpha beta using polyclonal rabbit antibodies to GpIb alpha and GpIb beta raised by immunization with purified glycocalicin and with synthetic peptide sequences from GpIb beta , respectively , as well as monoclonal antibodies directed against GpIX ( FMC 25 ) and against GpIb alpha ( AP 1 ) . ^^^ We performed two types of experiments , using either purified GpIb complex or platelets . ( 1 ) When wells were coated with nonreduced GpIb complex , the binding of FMC 25 was inhibited 73 % by GpIb alpha antibody , but only 30 % by the GpIb beta antibody ; when wells were coated with reduced complex , FMC 25 binding was inhibited by the same two antibodies by 86 % and 13 % , respectively . ^^^ When wells were coated with polyclonal GpIb alpha or GpIb beta antibodies and then incubated with reduced GpIb complex , only wells coated with GpIb alpha antibodies captured GpIX reactivity . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| We amplified the coding regions of GPIb alpha , GPIb beta , GPV , and GPIX from the patients ' genomic DNA with the polymerase chain reaction ( PCR ) and sequenced the PCR products . in case 1 , we identified a point mutation in the GPIX coding region that changes the codon for tryptophan 126 ( TGG ) to a nonsense codon ( TGA ) . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The complex , which serves as a platelet receptor for von Willebrand factor , is composed of 4 subunits : GPIb alpha , GPIb beta , GPIX , and GPV . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Lesions in the genes for GPIb alpha , GPIb beta or GPIX result in a bleeding diathesis , the Bernard Soulier syndrome ( BSS ) , which associates a platelet adhesion defect with thrombocytopenia , giant platelets and abnormal megakaryocytes ( MK ) . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Western blotting confirmed these results and showed moreover that there was a decreased disulfide bridge formation between GPIb alpha and GPIb beta . ^^^ After sequence analysis of the GPIb alpha , GPIb beta and GPIX genes , only a mutation in the GPIX gene at position 1826 ( A > G ) was identified , changing Asn 45 > Ser . ^^^ Our present observation of a decreased disulfide bridge formation between GPIb alpha and GPIb beta shows that GPIX is not only needed for the correct assembly of the complex but might also be needed for the disulfide bridge formation between GPIb alpha and GPIb beta . . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| These findings indicated that null expression of the abnormal GPIb beta causes decreased expression of the complex and results in the GPD phenotype in the patient , and suggested that homozygosity of the mutation may lead to a BSS phenotype in vivo . . ^^^ Immunoblot analysis showed normal electrophoretic mobility of GPIb alpha , GPIb beta , and GPIX . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The glycoprotein Ib ( GPIb ) complex is composed of GPIb alpha covalently attached to GPIb beta and noncovalently complexed with GPIX and GPV . ^^^ Patients with Bernard Soulier syndrome demonstrate that mutations in either GPIb beta or GPIX result in an absence of platelet GPIb alpha . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Tests with GPV transfectants revealed that the antibodies reacted with GPV independent of GPIb alpha , GPIb beta , or GPIX . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet membrane glycoprotein ( GP ) Ib 5 9 complex is the receptor for von Willebrand factor and is composed of four membrane spanning polypeptides : GPIb alpha , GPIb beta , GPIX , and GPV . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The platelet glycoprotein Ib ( GpIb ) complex is composed of four polypeptides : the disulfide linked GpIb alpha and GpIb beta and the noncovalently associated GpIX and GpV . ^^^ We have previously shown the presence of GpIb alpha and GpIb beta mRNA and protein in cultured human umbilical vein endothelial cells ( HUVECs ) as well as the presence of GpIb alpha mRNA and protein in tonsillar endothelium . ^^^ HUVEC GpIb beta , in contrast to its platelet counterpart , has a molecular weight of 50 kD and forms a correspondingly larger disulfide bonded complex with EC GpIb alpha . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Based on sequence homology to the known crystal structures of two other leucine rich repeat proteins , the human Nogo receptor and GPIb alpha , we propose a new structural model of GPIb beta . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Platelet glycoproteins Ib beta ( CD42c ) , 9 ( CD42a ) , and 5 ( CD42d ) , together with GP Ib alpha ( CD42b ) , form a receptor whose interaction with the von Willebrand factor is essential in the initial stages of haemostasis . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| Recent results with an adhesion blocking antibody ( RAM . 1 ) against GPIb beta , which is disulfide linked to GPIb alpha , have suggested a novel function of this subunit in regulating VWF mediated platelet adhesion , possibly involving its intracellular face . ^^^ A putative cooperation between the GPIb alpha and GPIb beta cytoplasmic domains was investigated by measuring the adhesion under flow to immobilized VWF of K 562 and Chinese hamster ovary ( CHO ) cells transfected with GPIb / ( 5 ) / IX containing mutations in this region . ^^^ These findings support a role of the GPIb beta intracellular domain in controlling the adhesive properties of the GPIb / V / IX complex through phosphorylation of GPIb beta Ser 166 and point to the existence of cross talk between the GPIb beta and GPIb alpha intracellular domains . . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| The complex consists of four polypeptides GPIb alpha , GPIb beta , GPIX and GPV . ^^^ |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P13224 and P07359 |
Pubmed |
SVM Score :0.0 |
| NA |
|