| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Four distinct peptide hydrolases ( EC 3 4 ) have been characterized in guinea pig epidermis ; these are cathepsin B 1 , cathepsin C , cathepsin D and arylamidase . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Separation and characterization of two acid proteases resembling cathepsin B 1 and cathepsin D and of an inhibitor of cathepsin B 1 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| To evaluate the effects of enzyme inhibitors , iodoacetate , p chloromercuribenzoate ( both specific for the endopeptidase , cathepsin B ( EC 3 . 4 . 22 . 1 ) ) and pepstatin ( specific for the endopeptidase , cathepsin D ( EC 3 . 4 . 23 . 5 ) were tested . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The degradation of cytosol proteins in vitro by purified cathepsin D and cathepsin B 1 and by mixtures of lysosomal enzymes , was studied . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Pepstatin inhibited cathepsin D , but did not affect cathepsin B and collagenolytic cathepsin . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D and cathepsin B both exhibited a zonal variation through the cartilage depth ; the surface cells appeared to contain more activity than those close to the subchondral bone . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D like and cathepsin B like acid protease activities were found to decrease throughout development , but the patterns of decrease were different for the two enzymes . ^^^ A gradual decrease was found for cathepsin D , whereas a sharp decrease between aggregates and migrating pseudoplasmodia was detected for cathepsin B . ^^^ By using microdissection techniques and fluorometric assays for amino acids and peptides , prestalk cells and prespore cells exhibited no difference in cathepsin D activity , whereas cathepsin B activity was higher in the prestalk cells . ^^^ Similarly , stalk cells and spores in the sorocarps showed no difference in cathepsin D activity , but showed a fivefold higher cathepsin B activity in the stalk cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The CaCl 2 extracted proteoglycan was subjected to proteolytic degradation by papain , trypsin , cathepsin D , cathepsin B , lysosomal elastase or cathepsin G . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The average size of the degradation products was least for cathepsin B and lysosomal elastase , and greatest for cathepsin D and cathepsin G . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and cathepsin D were purified from rat liver and skeletal muscle . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| At acid pH values cathepsin B also released hydroxyproline from human glomerular basement membrane but the lysosomal carboxyl proteinase , cathepsin D , had no action . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The specific activities of cathepsin D , cathepsin B , and neutral protease correlate closely with the fractional degradation rate . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Upon ion exchange chromatography multiple forms of acid proteinases emerged ; two of them were obviously identical with cathepsin D ( EC 3 . 4 . 23 . 5 ) , and two of them exhibited properties of cathepsin B ( EC 3 . 4 . 22 . 1 ) . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The bacteriolytic and bactericidal effects of the human proteinases cathepsin B , cathepsin D , cathepsin G , and elastase were investigated . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Further characterization of proteolytic enzymes in the inflamed synovial tissues showed the presence of cathepsin D , a neutral protease , and cathepsin B 1 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D activity was found to be normal in the disease , but the cathepsin B activity was decreased by 24 percent . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cartilage which had been incubated with either cathepsin D or cathepsin B 1 showed increased deformation in uniaxial compression perpendicular to the articular surface . ^^^ The proportion of the total collagen which was released from the cartilage was different for each enzyme : cathepsin D released between 0 and 1 . 5 per cent , cathepsin B 1 released between 2 . 3 and 4 . 3 per cent and collagenase released between 5 . 3 and 27 . 8 per cent of the collagen after 24 h . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The effect of three different concentrations of dimethoate on the activity of certain lysosomal enzymes , viz . beta glucuronidase , beta N acetylglucosaminidase , cathepsin B and cathepsin D in serum , skin , liver , kidney and spleen and the stability of liver and kidney lysosomes was studied in female albino rats . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B degraded apo MT approximately 36 fold more rapidly than cathepsin C and 45 fold more rapidly than cathepsin D . ^^^ Therefore , under the in vitro conditions used in this study , the relative potency of the cathepsins tested was cathepsin B much much greater than cathepsin C greater than cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D and cathepsin B 1 were used to digest the proteoglycan in some specimens , while in other specimens leukocyte elastase was used to attack the non helical terminal regions of the Type 2 tropocollagen molecules and possibly the Type 9 collagen molecule and thereby disturb the integrity of the collagen mesh . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The production of metastasis appears to involve a number of different proteases including the urokinase form of plasminogen activator , cathepsin B , cathepsin D and various metalloproteases . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Proteinase activities in rat thioglycollate elicited peritoneal cells and the cell free supernatant ( lavage fluid ) were measured by using the following substrates : Suc Ala Ala Pro Phe Methyl Coumarin Amide ( for cathepsin G or chymase ) , Suc Ala Ala Ala AMC ( for elastase or elastase like ) , Z Arg Arg AMC ( for cathepsin B ) , haemoglobin ( for cathepsin D ) and Ala AMC ( for alanine aminopeptidase : AAP ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsins are normally intracellular proteolytic enzymes associated with lysosomes ; however , when sections from Alzheimer brains were stained by antisera to cathepsin D and cathepsin B , high levels of immunoreactivity were also detected in senile plaques . ^^^ In situ enzyme histochemistry of cathepsin D and cathepsin B on sections of neocortex using synthetic peptides and protein substrates showed that senile plaques contained the highest levels of enzymatically active cathepsin . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The results of these experiments provide evidence that Ag presentation of OVA may be achieved through the action of two different classes of proteases : aspartyl proteases such as cathepsin D , which process OVA to antigenic fragments , and thiol proteases such as cathepsin B , which are important for expression of functional MHC 2 molecules by APC . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| A selective inhibitor of cathepsin B , a derivative of E 64 ( compound CA 074 ) , and pepstatin asialofetuin , a potent inhibitor of cathepsin D , were used for an in vivo study of the selective role of these proteinases in lysosomal proteolysis . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The concerted action of the endosomal proteases cathepsin D and cathepsin B can account for nearly all cleavages observed . ^^^ Cathepsin D appears to be mainly responsible for the initial cleavage of myoglobin , while cathepsin B catalyzes the C terminal trimming of initially released fragments . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| From information available in the literature on the substrate specificity of the three enzymes , it is clear that a cysteine is not accepted in any of the S 2 , S 1 , S 1 ' , and S 2 ' subsites of cathepsin B and L , and not in the S 1 and S 1 ' subsites of cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Increases in activities of muscle muticatalytic proteinase , modori inducing proteinase ( latent trypsin like proteinase ) , cathepsin B and L like proteases and cathepsin D were observed more markedly for male fish than female fish , in the spawning stage . 2 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Some of these vesicules also contained cathepsin B and / or cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| It has been shown that in vitro these protease inhibitors inhibit the activities of Ca ( 2+ ) dependent neutral proteases as well as lysosomal proteases , including cathepsin L , cathepsin b , and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| We identified three cleavage points for cathepsin B , corresponding to P ' 1 residues 532 , 795 , and 2487 ; four cleavage points for cathepsin L , corresponding to P ' 1 residues 2389 , 2452 , 2490 , and 2657 ; and four cleavage points for cathepsin D , corresponding to P ' 1 residues 551 , 1835 , 2468 , and 2643 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Radiolabeling the lumenal contents of macrophage endosomes confirmed that both the cysteine protease , cathepsin B , and the aspartyl protease , cathepsin D , were present in these vesicles . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The changes in the activities of certain lysosomal hydrolases , viz . , beta glucuronidase , beta N acetylglucosaminidase , beta galactosidase , beta glucosidase , alpha glucosidase , alpha galactosidase , alpha mannosidase , cathepsin B , cathepsin D , and collagenolytic cathepsin , in serum and heart of rats subject to myocardial infarction with isoproterenol , were studied during the periods of peak infarction and recovery . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The fragmentation pattern of cathepsin D treatment was different from that of cathepsin B , yielding 25 kDa intermediates . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| When cold acclimated mice were fasted at 21 degrees C , BAT protein loss was markedly enhanced and increases in cathepsin D and L activities were observed , but there was no change in cathepsin B and H and acid phosphatase specific activities . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Precursors of beta hexosaminidase , cathepsin B and beta galactosidase , which are routed to lysosomes via the mannose 6 phosphate ( Man 6 P ) receptor , were secreted in much lower amounts than pro cathepsin D , but their secretion was also increased by estradiol . ^^^ While estradiol stimulated gene expression of pro cathepsin D , it had no effect on that of pro cathepsin B . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Reduced protein breakdown was observed in the hypertrophic gland tissues , and was found to be inhibited by 20 mM epsilon amino n caproic acid , an inhibitor of serine protease , and 50 microM leupeptin , an inhibitor of trypsin , plasmin , papain and cathepsin B , but not by 2 mM PMSF ( phenylmethylsulfonyl fluoride ) , an inhibitor of serine protease , 10 microM pepstatin , an inhibitor of cathepsin D and 20 microM antipain , an inhibitor of cathepsin A and B . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| To investigate the role of proteolysis in amyloid formation , we studied the localization of the proteolytic enzymes , cathepsin D and cathepsin B , in the prefrontal cerebral cortex and hippocampus of human postmortem brains from patients with Alzheimer ' s disease and from individuals free of neurological disease . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsins A , B , C , and D were determined in the skin in various parts of the limb in 57 patients : in the lower third of the leg the activity of cathepsin D was increased by 183 . 8 % , that of cathepsin B by 140 . 2 % , and the activity of cathepsin B by 239 % . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| However , in 26 month old rats cathepsin B activity was unaffected , while cathepsin D was increased by gasoline administration . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting showed that both cathepsin B and cathepsin D were present in the Mr 28 , 000 32 , 000 electrophoretic bands . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cimaterol did not affect activities of cathepsin B , cathepsin D or neutral serine proteinase , but it reduced activities of the millimolar and micromolar forms of the CDP by 58 and 57 % , respectively , and it reduced activity of the inhibitor of the CDP ( calpastatin ) by 52 % . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| N acetyl beta glucosaminidase , aryl sulfatase , alkaline proteinase , cathepsin B , cathepsin D and aminopeptidase were detected in homogenate . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| To evaluate the potential of astrocytes as the source of these proteinases , cathepsin B ( CB ) and cathepsin D ( CD ) levels were measured in lysates of cultured astrocytes from neonatal rats . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Whereas cathepsin D immunoreactivity was confined to Mller ( glial ) cells , cathepsin B was demonstrated in some , but not all , neuronal cell types . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Because cathepsin D is capable of degrading a variety of proteins , the finding of high concentrations of the enzyme in AM and BALF from smokers , along with previous observations of elevated cathepsin B activity , suggests that lysosomal enzymes may cause or contribute to structural lung damage associated with cigarette smoking . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The catabolism of glycosaminoglycans was followed by estimating the activities of lysosomal glycohydrolases , namely beta glucuronidase , beta N acetylglucosaminidase , cathepsin B 1 , cathepsin B 2 and cathepsin D , in tissues and by estimating the urinary excretion and hexosamine and uronic acid . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The sequence immediately flanking the ATG start codon of the human alpha galactosidase A is highly homologous to sequences flanking the ATG start codons of the other human lysosomal hydrolases for which sequence information is available ( beta glucocerebrosidase , cathepsin B , cathepsin D , and beta hexosaminidase alpha chain ) , but not for any of the other 133 human signal peptides examined . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Plasmin , pronase , cathepsin D or cathepsin B each degraded the nonglycosylated and control fibrinogens similarly , as evidenced by the release of trichloroacetic acid soluble radioactivity and by SDS polyacrylamide gel electrophoresis and autoradiography of plasmic digests . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D , an acid proteinase , did not show a specific local activity pattern and was not influenced by the augmented growth hormone level in acromegaly , whereas cathepsin B , a neutral proteinase , showed its highest activity in the caudal prolongation and the posterior area and was significantly increased in all areas in acromegaly . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Maximal activity of each protease occurs almost routinely at passage 5 or 9 , reaching values of approx . 1400 , 0 . 77 , 4520 nmol min 1 per mg protein for neutral exopeptidase ( passage 5 ) , neutral endopeptidase ( passage 5 ) and cathepsin B ( passage 5 ) respectively , and 7 . 1 micrograms trichloroacetic acid soluble peptide min 1 per mg protein for cathepsin D ( passage 15 ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Activities of cathepsin B and N acetyl beta glucosaminidase , but not cathepsin D , were elevated in the soleus muscles of clenbuterol treated rats . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Both cathepsin B and cathepsin D activities could be induced by growth of K 562 cells in medium containing either hemin or heat treated serum or by increasing the concentrations of untreated serum in the medium . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In order to obtain information about the changes in lysosomal enzyme activities in arterial endothelial cells under hypertensive conditions , a biochemical study was performed on 5 lysosomal enzymes , acid phosphatase , N acetyl beta glucosaminidase ( NAGase ) , cathepsin B , cathepsin D and beta glucuronidase , in endothelial cells isolated by an enzymatic technique from the aorta of spontaneously and renal hypertensive rats , and normotensive control rats . ^^^ The differences in the activities of NAGase , cathepsin B and cathepsin D between hypertensive and control animals increased markedly with advancing age . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The tissue distribution of mRNAs encoding two lysosomal proteases , cathepsin B and cathepsin D , was examined using cloned cDNAs to probe Northern and dot blots of RNAs extracted from various rat tissues . ^^^ Cathepsin B mRNA showed a wide range of variation in expression in the tissues analyzed with the highest concentrations found in spleen and kidney , while the cathepsin D mRNA levels were relatively uniform in these same tissues . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Amongst this broad spectrum of small lysosomes the distribution of cathepsin B , together with other acid hydrolases is associated with the more rapidly sedimenting lysosomes whilst cathepsin D differs in being associated with the slowest sedimenting lysosomes . ^^^ Cathepsin B ( and also cathepsin D and acid ribonuclease ) appears to be associated only with lysosomes of high density . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Hydrocarbons also caused extracellular release of the lysosomal enzymes cathepsin D ( EC 3 . 4 . 23 . 5 ) and cathepsin B ( EC 3 . 4 . 22 . 1 ) in a manner corresponding with cell damage . ^^^ There was more cathepsin D activity released from cells than cathepsin B . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B activity was higher in cirrhotic liver samples as compared with the controls , whereas the increased activity of cathepsin D was not significant . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In homogenates of malignant tissues the activities of collagenase , cathepsin B , cathepsin ( B+L ) , cathepsin H and cathepsin D were significantly higher than in D NM tissues . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Action of brain cathepsin B , cathepsin D , and high molecular weight aspartic proteinase on angiotensins 1 and 2 . ^^^ The action of three previously isolated electrophoretically homogeneous brain proteinases cathepsin B ( EC 3 . 4 . 22 . 1 ) , cathepsin D ( EC 3 . 4 . 23 . 5 ) , and high molecular weight aspartic proteinase ( Mr = 90K ; EC 3 . 4 . 23 . ) on human angiotensins 1 and 2 has been investigated . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Procathepsin D was then incubated in vitro ( 40 degrees C , 1 240 min ) with active cathepsin D , papain , and cathepsin B , either singly or sequentially , and the reaction products analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two dimensional electrophoresis . ^^^ Only the Mr 48 , 000 , pI 6 . 4 products of papain and cathepsin B mediated proteolysis comigrated with the most basic isoform of active cathepsin D found in cardiac tissue . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activity and subcellular localization of leucine aminopeptidase , cathepsin B and cathepsin D in the immature and mature placenta ] . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Eighteen clones of a methylcholanthrene induced murine fibrosarcoma ( 3AM ) which were heterogeneous with respect to metastatic potentials and in vivo growth rates were examined for five different protease activities : acid protease ( cathepsin D ) , BANA hydrolase ( cathepsin B ) , neutral protease , collagenase , and plasminogen activator . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Epidermal transglutaminase may be activated by lysosomal acid proteinases , such as cathepsin B 1 and cathepsin D , which are released and activated during the autolytic stages in granular layer in epidermis . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The procedure allowed for the simultaneous isolation of cathepsin B and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| None of the tumours were positive for lysozyme , alpha 1 antitrypsin to cathepsin B whereas 12 lesions gave a granular reaction for cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Changes in cardiac cathepsin B activity in response to interventions that alter heart size or protein metabolism : comparison with cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activity of collagenase , cathepsin B 1 , cathepsin D and Hyaluronidase was determined in skin , bone , liver , kidney , spleen and serum of adjuvant induced arthritic rats during the acute and chronic phase of the disease . ^^^ Prednisolone and L thyroxine were administered to arthritic rats and the activity of collagenase , cathepsin B 1 , cathepsin D and hyaluronidase was determined in the treated groups during the acute and chronic phase of the disease . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The presence of cathepsin D , plasminogen activator , cathepsin B , cathepsin G and elastase like enzymes was observed . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| ATP , creatine phosphate , cAMP , and activities of cathepsin B 1 , cathepsin D , and alkaline protease were not different in muscles of the uremic versus sham rats . ^^^ The enhanced protein degradation does not appear to be due to increased muscle cathepsin B 1 , cathepsin D , or alkaline protease activities . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activity of collagenase and certain lysosomal hydrolases ( cathepsin B 1 , cathepsin D , beta glucuronidase and beta N acetyl glucosaminidase ) was studied in serum and tissues of rats with streptozotocin or alloxan induced diabetes . ^^^ Comparison of the alloxan or streptozotocin treated groups with control animals showed an increase in lysosomal enzymes ( cathepsin B 1 , cathepsin D , beta glucuronidase and beta N acetyl glucosaminidase in skin , liver and spleen ) ( p less than 0 . 05 ) but beta N acetyl glucosaminidase was unchanged in the spleen of streptozotocin diabetic rats . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Among these tissues , the uvea and retina show the higher enzyme activities of acid phosphates , beta blucuronidase , alpha fucosidase , alpha mannosidase , arylsulfatase , cathepsin D , cathepsin B and others . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Nanogram quantities of trypsin , chymotrypsin , elastase and cathepsin D can be detected , but the substrate is unaffected by cathepsin B . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Rabbit thyroids contain cathepsin D ( CD ) and several thiol endopeptidases including cathepsin B and three newly described enzymes ( cathepsins 180K , 110K , and 45K ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The enzymes studied were prolyl endopeptidase ( EC 3 . 4 . 21 . 26 ) , a soluble metalloendopeptidase , pyroglutamyl peptide hydrolase ( EC 3 . 4 . 11 . 8 ) , a multicatalytic protease complex , cathepsin B ( EC 3 . 4 . 22 . 1 ) , cathepsin D ( EC 3 . 4 . 23 . 5 ) , aminopeptidase ( EC 3 . 4 . 11 . 2 ) , and a membrane bound neutral metalloendopeptidase ( EC 3 . 4 . 24 . 11 ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Muscle ATP , creatine phosphate , cyclic AMP , and activities of cathepsin B 1 , cathepsin D , and alkaline protease were not different in the uremic and sham rats . ^^^ The increased protein degradation does not appear to be due to enhanced activities of muscle cathepsin B 1 , cathepsin D or alkaline protease . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activities per microgram DNA of five lysosomal enzymes [ cathepsin D , cathepsin B , beta N acetylglucosaminidase ( beta NAG ) , beta glucuronidase , and acid phosphatase ] were measured in homogenates of female and male rat ( Sprague Dawley ) hearts . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Pepstatin , which inhibits cathepsin D , and leupeptin , which inhibits cathepsin B , did not significantly inhibit the elastase like activities in these cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Muscle ATP , creatine phosphate , and cyclic AMP , and muscle cathepsin B 1 , cathepsin D , and alkaline protease activities were not different in the uremic and control rats . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Similar levels of acid phosphatase , beta glucuronidase and cathepsin D were observed in V 79 and CQ 22 cells , but the level of cathepsin B was lower in the mutant . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| If one believes that lysosomal cathepsin D and cathepsin B act synergistically with collagenase , it can be assumed that PGE 2 is involved in a lysosomal degradation of the connective tissue . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Other proteinases ( cathepsin B , cathepsin D , trypsin and chymotrypsin ) also decreased aldolase activity toward fructose 1 , 6 bisphosphate more rapidly in the presence of ATP and more slowly in the presence of Pi . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The presence of the lysosomal proteinases cathepsin B and cathepsin D at extracellular sites in rheumatoid synovium was demonstrated using the antibody capture technique . ^^^ Unlike cathepsin D , the cysteine proteinase cathepsin B was commonly detected only at the edges of the synovial explants . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Sephadex G 75 column chromatography resulted in apparent molecular weights of 25 , 000 ( cathepsin B ) and 35 , 000 ( cathepsin D ) . ^^^ Fluorescence quenching experiments showed that tryptophyl residues of cathepsin B were less occluded and located in a more electronegative microenvironment that those pertaining to cathepsin D . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B 1 in suckling rats is markedly lower than in adults , while cathepsin D is only moderately lower and cathepsin L does not significantly differ . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The in vivo regulation of three acid hydrolases , namely cathepsin D , cathepsin B , and acid phosphatase , by GH was investigated . ^^^ A degree of specificity in this regulation was apparent because cartilage cathepsin B , unlike cathepsin D and acid phosphatase , was refractory to hypophysectomy and GH treatment . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The only proteinases to date that have been shown to be present in muscle cells and capable of degrading myofibrillar proteins are Ca2+ activated proteinase , cathepsin B , and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The specific activity of cathepsin B 1 decreases progressively in the liver during the first week after transplantation reaching one third of the initial levels , whereas that of cathepsin D shows the opposite behaviour increasing to levels 40 % higher than in controls . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| When cathepsin D was inhibited by pepstatin , the proteolysis of 125I labeled low density lipoproteins was inhibited by more than 90 % , whereas when cathepsin B was inhibited by leupeptin , the rate of proteolysis decreased by approx . 50 % . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Injection of leupeptin also caused a marked increase in the activities of free lysosomal proteases , such as cathepsin B ( EC 3 . 4 . 22 . 1 ) , cathepsin L ( EC 3 . 4 . 22 . ) , cathepsin D ( EC 3 . 4 . 23 . 5 ) and lysosomal carboxypeptidase A in the cytosol fraction . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In this paper , the presence of cathepsin D , cathepsin B , lysosomal elastase and cathepsin G in rheumatoid synovium is established by isolation , purification , and characterization of these proteases . 2 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Injection of leupeptin also caused marked increase in the activities of free lysosomal proteinases , such as cathepsin A and cathepsin D and moderate increase of cathepsin B and cathepsin L . ^^^ Purified cathepsin A ( a serine proteinase ) , cathepsin B and cathepsin L ( thiol proteinase ) are potent inactivators of aldolase but cathepsin H and cathepsin D are not . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activities of three lysosomal enzymes ( cathepsin B 1 , cathepsin D , and acid phosphatase ) and thymidylate synthetase were measured in implanted mammary tumors as well as in the livers of host animals that were either euthyroid , hypothyroid , or hyperthyroid . ^^^ In the livers of the host animals , hypothyroidism produced a significant decrease in cathepsin B 1 and a significant increase in acid phosphatase but did not change cathepsin D or thymidylate synthetase levels . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The myocardial hypertrophy was accompanied by a rise in the activity of cathepsin D but not of cathepsin B ; this was correlated with an increase in cathepsin D rich interstitial cells while the number of cathepsin D positive lysosomes in myocytes was decreased , as assessed from immunohistochemistry . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| A review of continuing studies on the physiological and pathological roles of the lysosomal proteinases , cathepsin D and cathepsin B is presented . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The total activities of cathepsin B and cathepsin H in pectoral muscle of dystrophic chickens ( Line 413 ) were about two times higher than in control chickens ( Line 412 ) , and cathepsin D activity was about 3 times higher in this muscle in the dystrophic chickens . ^^^ When E 64 c , a synthesized potent thiol inhibitor was injected subcutaneously , in various doses , daily for 80 days into dystrophic chickens ( L 413 ) , the activities of cathepsin B and cathepsin H were reduced to the levels in control chickens ( Line 412 ) , but cathepsin D activity , which is insensitive to E 64 c in vitro , was not changed . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The in vitro activities of cathepsin B 1 and cathepsin D were inhibited by all the gold compounds . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Median cathepsin D values were 27 pmol ( tumour tissue ) vs . 12 pmol ( normal tissue ) per mg of total protein , median cathepsin B values were 1 . 25 micrograms / mg ( tumour tissue ) vs . 0 . 23 micrograms / mg ( normal tissue ) and median cathepsin L values were 39 . 8 ng / mg ( tumour tissue ) vs . 20 . 0 ng / mg ( normal tissue ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| We have studied the roles of three different acidic proteases , cathepsin D , cathepsin B and cathepsin L , in the processing of ovalbumin ( OVA ) for presentation in the context of 1 Ad . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Gelatin degrading activity of live mesangial cells was also determined . mRNA levels for collagenase 4 , cathepsin B , and cathepsin D were determined by Northern analysis . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| After 135 days the free , latent and total activities of the lysosomal enzymes cathepsin B and cathepsin D in gastrocnemius muscle were unaffected by the induction of experimental cirrhosis when expressed relative to tissue wet weight , protein or DNA . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D , cathepsin B and to a lesser extent other cathepsins have been described as prognostic markers in cancer . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Both cathepsin B and cathepsin D are necessary for processing of ovalbumin as well as for degradation of class 2 MHC invariant chain . ^^^ Both CA 074 ( a specific inhibitor of cathepsin B ) and pepstatin A ( a specific inhibitor of cathepsin D ) showed an inhibitory effect on the IL 2 production from an OVA specific , 1 Ad restricted helper T ( Th ) cell clone upon stimulation with OVA presented by the 1 Ad positive APC . ^^^ First , not only aspartyl protease , such as cathepsin D , but also thiol protease , such as cathepsin B , is involved in antigen processing by APC . ^^^ Second , both cathepsin B and cathepsin D are necessary for degradation of the invariant chain ( 2 ) from the MHC class 2 alpha beta heterodimer in endosomes in order to express functional MHC class 2 molecules for binding antigenic peptides . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Here , we investigated the regulation of protease ( cathepsin B , cathepsin D , proteasome C 2 subunit and m calpain ) mRNA concentrations by Dex in cultured L 8 muscle cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| When we extended this study with purified cathepsins , we obtained the same answer , i . e . , the ability of different cathepsins to degrade apo MT was in the following order : cathepsin B > > cathepsin C > cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Using purified cathepsins B and D , we demonstrated that covalent binding of C3b to TT totally abolished TT proteolysis by cathepsin D , while proteolysis by cathepsin B was preserved . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Our data show that the differentiation of HT 29 colon carcinoma cells into mucus secreting cells impairs the secretion of plasminogen activator and cathepsin D , but does not affect cathepsin B . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The effects of E 64 ( Cathepsin B and L inhibitor ) and Pepstatin A ( Cathepsin D inhibitor ) on spontaneous and experimental metastasis formation were investigated in mice with MCa mammary carcinoma , M 5076 ovarian sarcoma and L 1210 leukemia . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D and cathepsin B are endosomal / lysosomal proteases that are thought to play a role during in vivo antigen processing , releasing fragments for binding to major histocompatibility complex class 2 products and subsequent presentation to T cells . ^^^ Cathepsin D , but not cathepsin B , was shown to release fragments from reduced or non reduced FMDV under mild conditions in vitro . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin D , but not cathepsin B , releases T cell stimulatory fragments from lysozyme that are functional in the context of multiple murine class 2 MHC molecules . ^^^ In this study , the major endosomal / lysosomal proteases cathepsin D and cathepsin B were tested on their ability to release T cell stimulatory peptides from hen egg white lysozyme ( HEL ) in vitro . ^^^ Whereas neither enzyme could cleave unreduced HEL under mild conditions , reduced HEL was readily cleaved by cathepsin D but not by cathepsin B . ^^^ Instead , cathepsin B was found to be very active in the trimming of HEL peptides after their release by cathepsin D . ^^^ Our data suggest that cathepsin D rather than cathepsin B may play a central role in the initial release of HEL fragments during endosomal / lysosomal processing . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Positive granular cytoplasmic staining was detected for cathepsin D in 90 % of the tumors , for cathepsin B in two thirds of the tumors , and for cathepsin L in approximately one half of the tumors . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| However , a trace level of cathepsin D , added to the assay for peptide binding in the presence of cathepsin B , further enhanced peptide binding about three times . ^^^ These experiments support an hypothesis for the staged release of 2 fragments by cathepsin D and cathepsin B , catalyzing at one point the insertion of a peptide into the antigen binding site formed by class 2 alpha and beta chains . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| We studied the levels of two lysosomal proteinases implicated in the proteolysis of myelin basic protein , cathepsin B ( CB ) and cathepsin D ( CD ) , in peripheral blood mononuclear cells ( PBMCs ) of 20 stable relapsing remitting MS patients . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Moreover , the multinucleated cells isolated from these tumors had decreased mRNA levels for cathepsin B , cathepsin D , and tartrate resistant acid phosphatase ( TRAP ) as well as secreted cathepsin B and TRAP enzyme activity in response to treatment with 10 nM 17 beta E 2 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Inhibition of cathepsin D reduces ricin A chain cytotoxicity , while blocking cathepsin B enhances cytotoxicity . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Adsorption and helical coiling of amphipathic peptides on lipid vesicles leads to negligible protection from cathepsin B or cathepsin D . ^^^ Cathepsin B or cathepsin D cleavages of three such peptides were identified . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| When the proenzyme was incubated with the cathepsins B and D free tritosomal contents , prepared by treatment of the cathepsin B free tritosomal contents with anti cathepsin D IgG Sepharose , the processing and activation did not occur . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The effect of curcumin on lysosomal hydrolases in serum and heart was studied by determining the activities of beta glucuronidase , beta N acetylglucosaminidase , cathepsin B , cathepsin D , and acid phosphatase . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| DESIGN Using immunohistochemical analysis of small cell carcinomas , the tissue expression of corticotropin , bcl 2 , p glycoprotein , cathepsin B , cathepsin D , CD 44 , carcinoembryonic antigen , collagenase 4 , Leu 7 , neu oncoprotein , p 53 , S 100 , and synaptophysin was assessed . ^^^ RESULTS Compared with the control group of short term survivors , tumors from prolonged survivors were unique in their relative absence of staining for cathepsin B ( 0 / 13 vs 3 / 13 [ 23 % ] , P = . 037 ) , cathepsin D ( 5 / 13 [ 38 % ] vs 13 / 15 [ 87 % ] , P = 0 . 006 ) , carcinoembryonic antigen ( 5 / 13 [ 38 % ] vs 11 / 15 [ 73 % ] , P = . 047 ) , and neu oncoprotein ( 5 / 13 [ 38 % ] vs 14 / 15 [ 93 % ] , P = . 0014 ) . ^^^ CONCLUSIONS Negative immunohistochemical staining for cathepsin B , cathepsin D , carcinoembryonic antigen , and neu oncoprotein is associated with prolonged survival in small cell carcinoma of the lung . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| During dexamethasone administration , a significant decrease in both the free and total activities of beta glucuronidase , beta N acetyl glucosaminidase , beta galactosidase , alpha galactosidase , alpha mannosidase , cathepsin B and cathepsin D are observed in heart and kidney , but the enzyme levels are shown to increase in serum . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Activation of cathepsin B , secreted by a colorectal cancer cell line requires low pH and is mediated by cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| At both levels , concentrations of uPA were significantly correlated with those for uPAR . uPA levels also correlated significantly with cathepsin B and cathepsin D but not with cathepsin L , MMP 8 or MMP 9 levels . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| To elucidate involvement of proteinases in malignancy of keratinocytes , expression of cathepsin B , a cysteine proteinase , and cathepsin D , an aspartic proteinase , was ascertained in formalin fixed paraffin embedded specimens of normal skin , squamous cell carcinoma ( SCC ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| However , the modified species was more susceptible to proteolysis by bovine spleen cathepsin B and cathepsin D than the unmodified species . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsins B , L , H , and S readily cleave BGP at the G 7 A8 bond ; cathepsin L also cleaves at R 43 R44 ; cathepsin B also cleaves at R 44 F45 ; and cathepsin D cleaves only at A 41 Y42 . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| To clarify the significance of the constituents of canine senile plaques ( SPs ) or cerebrovascular amyloid deposits , paraffin and cryostat sections of canine brains were examined by immunohistochemistry using antibodies against cathepsin B ( CB ) , cathepsin D ( CD ) , cystatin C ( CC ) , alpha 1 antichymotrypsin ( ACT ) , heat shock protein 70 ( HSP 70 ) , ubiquitin ( Ubq ) , and apolipoprotein E ( Apo E ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| As cathepsin D was found to cleave the betaAPP 100 FLAG in the vicinity of the C terminus of the beta amyloid peptides and cathepsin B has a high carboxypeptidase activity at low pH , the possibility can not be excluded that cathepsins D and B are involved in the amyloidogenic processing of APP . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , mature and proenzyme forms of the proteases cathepsin B and cathepsin D , a candidate APP secretase , were identified in most early endosomes in Alzheimer brains but were detectable in only a minor proportion of endosomes in normal brain . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| By using total RNA isolated from primary cultures of Sertoli , Leydig , and germ cells for reverse transcription and polymerase chain reaction ( RT PCR ) , the mRNAs of cathepsin B , C , D , H , L , and S were shown to be expressed by Sertoli and Leydig cells , whereas germ cells isolated from adult rats expressed all of the above cathepsin mRNAs except cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrate that cysteamine suppresses cathepsin B mRNA levels and immunoreactive protein in cultured astroglia , whereas cathepsin D mRNA and protein levels are significantly augmented by CSH exposure in these cells . ^^^ Moreover , cathepsin D ( but not cathepsin B ) exhibits robust colocalization to the red autofluorescent inclusions . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| To determine the involvement of proteinases with hydrolytic activity towards extracellular matrix and basement membrane , in invasion and metastasis of tumour cells , the expression of cathepsin D , an aspartic proteinase , and cathepsin B , a cysteine proteinase , was studied . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Standard indirect immunoperoxidase methods were used to identify matrix metalloproteinases ( MMP 1 , MMP 3 , MMP 9 ) , cathepsin B , cathepsin D , platelet endothelial cell adhesion molecule , cytokeratins , smooth muscle actin , CD 68 , and factor 8 related antigen . ^^^ By contrast , neither cathepsin B nor cathepsin D were present , although both were seen in uterine macrophages and stromal cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we used immunohistochemical techniques to detect the distribution of matrix proteolytic enzymes in periprosthetic tissue , including lysosome enzymes ( cathepsin B , cathepsin D and cathepsin G ) , and matrix metalloproteinase ( MMPs , MMP 1 , MMP 2 , MMP 3 ) . ^^^ The results showed that cathepsin B , cathepsin D and cathepsin G were found in most fibroblasts and macrophage like cells , including multinuclear giant cells and epithelioid cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Some proteolytic enzymes , trypsin , cathepsin B , cathepsin D , collagenase , elastase and their inhibitors , API and AMG , in serum of patients with colorectal carcinoma have been evaluated . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Several extended peptide substrates for the human liver enzymes cathepsin B and cathepsin D have been selected as cleavable linkers for lysosomal proteolysis of bioconjugates . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| When the active forms of cathepsins B and D were examined during the apoptotic process of wild type cells , the amount of cathepsin B was drastically reduced 24 hr after the onset of culture , whereas that of cathepsin D considerably increased . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B , cathepsin D , and caspase 3 failed to produce either the 53 kD or 57 kD NF BDPs . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate directly that one of the catalysts for the intraoocytic processing of VTG in yolk proteins is cathepsin D ; however , we can not exclude also a role of cathepsin B in the same process . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| It was slightly suppressed by N ( L 3 trans propylcarbamoyloxirane 2 carbonyl ) L isoleucyl L prol ine ( CA 074 ) , a selective inhibitor of cathepsin B , and by pepstatin , an inhibitor of cathepsin D , whereas it was markedly suppressed by a combination of cystatin alpha and either CA 074 or pepstatin . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| At 3 h after the onset of culture , the immunoreactivity for cathepsin B slightly decreased in the cells , while immunodeposits for cathepsin D in the cells became more intense and larger in size than those at 0 h . ^^^ Corresponding to the increase in the number of TUNEL positive cells at 12 h and 24 h , the immunoreactivity for cathepsin B was drastically diminished in the cells , whereas that for cathepsin D was significantly augmented , especially in TUNEL positive cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The Golgi protein galactosyltransferase and the lysosomal proteins cathepsin B and cathepsin D appear at the plasma membranes during sustained secretomotor stimulation . ^^^ MHC 2 cycle through endomembrane compartments which contain La , galactosyltransferase , cathepsin B and cathepsin D and which are sites of proteolysis . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The parameters Cathepsin B , Cathepsin D , Collagenase 4 , Metallothionein and PCNA were selected from previous studies on small collectives . ^^^ Particularly conspicuous was the expression of Cathepsin B , Cathepsin D and Collagenase 4 at the dermal invading front of the tumors . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The proteolytic enzymes collagenase 4 , cathepsin B , and cathepsin D proved to be significant prognostic factors . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical expression of MIB 1 , Bcl 2 , cathepsin B , cathepsin D , basic fibroblast growth factor ( bFGF ) , c met , and type 4 collagenase were studied on formalin fixed tissue from 33 nonconsecutive cases of pheochromocytoma , selected on the basis of reliable long term follow up , to determine associations with malignancy . ^^^ C met , bFGF , cathepsin B , cathepsin D , and collagenase were strongly expressed in most tumors and were not predictive of outcome , nor was bcl 2 , which was variably expressed . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| For example , proteinases expressed by the various stages of the schistosome life cycle , in particular the well characterized cercarial elastase which is involved in the penetration of the host skin and the variety of proteinases , such as cathepsin B ( Sm 31 ) , cathepsin L 1 , cathepsin L 2 , cathepsin D , cathepsin C and legumain ( Sm 32 ) , which are believed to be involved in the catabolism of host haemoglobin . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Leupeptin and E 64 dose dependently inhibited both cathepsin B and cathepsin L activities , and pepstatin A strongly inhibited cathepsin D activity in rat kidney lysosomes . ^^^ The present results suggest that cathepsin B , cathepsin L , and cathepsin D in kidney lysosomes are involved in the metabolic degradation of human IL 1beta . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The initiation of cell death by CA 074 or the cathepsin B antisense were inhibited by the addition of pepstatin A , a lysosomal aspartic proteinase inhibitor , or by cathepsin D antisense . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| They showed strong inhibitory activities only to cysteine proteinases such as calpain , papain , cathepsin B , and cathepsin L and not to serine ( e . g . thrombin and cathepsin G ) and aspartic proteinases ( e . g . cathepsin D ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In the vas deferens , these cells were intensely reactive for cathepsin A and SGP 1 and unreactive for cathepsin B ; in the case of cathepsin D , basal cells were weakly reactive in the proximal vas deferens but intensely reactive in the middle and distal vas deferens . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In this tissue , the occurrence of cathepsin B , a lysosomal cysteine proteinase , and cathepsin D , a lysosomal aspartic proteinase , has been described recently ( Gerhartz et al . , Comp Biochem Physiol , 118B : 159 166 , 1997 ) . ^^^ Determination of cathepsin B like and cathepsin D like proteolytic activity in the yolk sac membrane indicated a significant correlation between growth of the yolk sac membrane and proteolytic activity , shown by an almost constant specific activity . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The present study examines age related changes in the subcellular localization of cathepsin B ( cath B ) and cathepsin D ( cath D ) , as well as morphological features of the cathepsin immunoreactive ( ir ) neurons in rat cerebral cortex . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Our purpose was to analyze the predictive relevance of DNA ploidy , S phase fraction ( SPF ) , and tissue levels of lysosomal proteinases cathepsin D ( CD ) , cathepsin B ( CB ) , cathepsin L ( CL ) , and urokinase type plasminogen activator ( uPA ) and that of the intracellular cysteine proteinase inhibitor stefin A on clinical outcome . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Significant correlation was found for cathepsin D ( P < . 041 ) and borderline correlation for cathepsin B ( P < . 055 ) but not for cathepsin L . ^^^ With respect to prognosis of patient survival , only tumor cell associated cathepsin D ( P = . 042 ) and myoepithelial cell associated cathepsin B ( P = . 061 ) showed borderline significance . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B , a prognostic indicator in lymph node negative breast carcinoma patients : comparison with cathepsin D , cathepsin L , and other clinical indicators . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Antigen levels of uPA and PAI 1 were determined by ELISA in detergent extracts ; cathepsin B , D , and L content was determined in cytosol fractions of the primary tumor : cathepsin D by ELSA and cathepsin B and L by ELISA . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Age related lysosomal changes as seen with cathepsin B immunocytochemistry were considerably more subtle than those obtained with cathepsin D antibodies . ^^^ In contrast , a set of glial and / or vascular elements located in a distal dendritic field of the middle aged hippocampus was much more immunoreactive for cathepsin B than cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Lysosomal cathepsin B plays an important role in antigen processing , while cathepsin D is involved in degradation of the invariant chain inovalbumin immunized mice . ^^^ Thus , cathepsin B appears to process antigens directed to preferential activation of Th 2 cells , while cathepsin D may be responsible for the degradation of 2 , the processing of which is essential in initiating the antigen specific activation of Th 1 and Th 2 CD4+ T cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Two proteinases that occur in the precursor macrophages , cathepsin B , a thiol proteinase related to cathepsin K , and an unrelated lysosomal aspartate proteinase , cathepsin D , were also studied to determine the specificity of the differentiation events . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The major proteases detected were matrix metalloproteases ( MMP 9 , MMP 2 , and MMP 1 ) , cathepsin B , cathepsin D , and the mast cell serine proteases , tryptase and chymase . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Whereas cathepsin B immunopositivity was absent from glandular epithelial cells of healthy subjects and only slightly present in SS patients , cathepsin D immunoreactivity was considerably greater ( p < 0 . 0001 ) in both the acini and the ducts of patients with SS as compared with control subjects . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Effect of three epsilon aminocaproylaminoacids with significant antifibrinolytic activity on chymotrypsin , trypsin , cathepsin B , cathepsin C and cathepsin D activities was examined . ^^^ All tested dipeptides did not influence activities of cathepsin B , cathepsin C and cathepsin D . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In relation to the induction of autophagy in PC 12 cells following serum deprivation , immunoreactivity , protein amounts , and the proteolytic activity of lysosomal proteinases , particularly cathepsins B and D , are all greatly altered ; those of cathepsin B drastically decrease in the cells from the early stage of serum deprived cultures , whereas those of cathepsin D increase . ^^^ Moreover , PC 12 cells overexpressing cathepsin D undergo apoptosis more rapidly in serum deprived cultures than wild type cells , whereas those overexpressing cathepsin B increase the viability . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In this study we cloned three putative yolk processing enzymes ; specifically , cathepsin B and L , and lipoprotein lipase ( LPL ) , from the rainbow trout ovary and determined their patterns of gene expression , together with cathepsin D , during oogenesis and embryogenesis using reverse transcription polymerase chain reaction . ^^^ Quantitatively , expression of cathepsin D mRNA was higher than cathepsin B , cathepsin L , and LPL mRNA throughout the period studied . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Treatment with E 64 or CA074Me ( 2 specific inhibitors of cathepsin B ) or with pepstatin A ( a specific inhibitor of cathepsin D ) was cytotoxic for 2 neuroblastoma cell lines having different degrees of malignancy . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Since HOCl modified LDL has been detected in vivo , the effects of HOCI modified LDL on the activities of the cysteine protease cathepsin B and the aspartyl protease cathepsin D were investigated . ^^^ LDL ( 0 . 5 mg protein / ml ) , which had been exposed to HOCl ( 25 200 microM ) , caused rapid dose dependent inactivation of cathepsin B , but not of cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| A total of 36 patients with lung cancer referred for curative resection and 10 control subjects had biopsies of latissimus dorsi muscle taken at operation . mRNA levels of four components of the ubiquitin proteasome pathway , i . e . polyubiquitin , C 2 alpha proteasome subunit , 14 kDa ubiquitin carrier protein and ubiquitin activating protein , and of two lysosomal proteolytic enzymes , i . e . cathepsin B and cathepsin D , were measured using quantitative Northern blotting . mRNA levels for cathepsin B , but not for components of the ubiquitin proteasome pathway , were higher in patients with cancer compared with controls ( P=0 . 01 ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In this study , the activities of the lysosomal cysteine proteases cathepsin B and H , dipeptidyl peptidase 1 , and the serine protease tripeptidyl peptidase 1 and dipeptidyl peptidase 2 , all ascribed a role in collagen digestion , were compared with those of the aspartate protease cathepsin D , and lysosomal glycosidases in leukocytes from rheumatoid arthritis patients at different stages of the disease . ^^^ In all patients the activities of cysteine protease cathepsin B , dipeptidyl peptidase 1 , aspartate protease cathepsin D , and two glycosidases were elevated , but the activities of the serine proteases tripeptidyl peptidase 1 , dipeptidyl peptidase 2 , and the cysteine protease cathepsin H was unchanged . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The following graduation of expression was determined : cathepsin D > cathepsin L > cathepsin B . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Reperfusion after exposure to H ( 2 ) O ( 2 ) caused a marked increase in caspase 3 and cathepsin D activities and a marked decrease in cathepsin B activity . ^^^ Pepstatin A and CA 074 Me inhibited cathepsin B and cathepsin D activities , respectively , in the presence and absence of Ac DMQD CHO . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Synthetic peptides from the excretory secretory enzymes , cathepsin B ( Sm 31 ) legumain ( Sm 32 ) and cathepsin D ( Sm 45 ) , have been synthesized . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , conjugation with CMD enhanced the resistance of beta LG to cathepsin B and cathepsin D , which suggest that conjugation with CMD inhibited the degradation of beta LG by proteases in APC and led to suppression of the generation of antigenic peptides including T cell epitopes from beta LG . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Larvae of this species were found to use a complex proteolytic system that includes cathepsin D , cathepsin B , trypsin , and chymotrypsin like activities . ^^^ Trypsin like activity was evenly distributed among the anterior , middle , and posterior portions of the gut , whereas cathepsin B and cathepsin D like activities were mainly located in the anterior and middle sections , and the chymotrypsin like activity was highest in the middle and posterior sections . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Pepstatin A , an inhibitor of cathepsin D , also decreased mitochondrial depolarization , caspase 3 activation , and caspase 9 activation , whereas leupeptin , an inhibitor of cathepsin B , had no effect . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The levels of expression of cathepsin D and of cathepsin B correlated significantly with the levels of expression of RARbeta . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| First , inhibition of cathepsin B , and not of caspases or other proteases , such as cathepsin D or calpains , results in a strong protection against drug induced cell death in several NSCLC cells . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Our aza peptide epoxides designed for caspases are stable , potent , and specific inhibitors , as they show little to no inhibition of other proteases such as the aspartyl proteases porcine pepsin , human cathepsin D , plasmepsin 2 from P . falciparum , HIV 1 protease , and the secreted aspartic proteinase 2 ( SAP 2 ) from Candida albicans ; the serine proteases granzyme B and alpha chymotrypsin ; and the cysteine proteases cathepsin B and papain ( clan CA ) , and legumain ( clan CD ) . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Cathepsin B and cathepsin D expression in the progression of colorectal adenoma to carcinoma . ^^^ Lysosomal proteinases , cathepsin B ( CB ) and cathepsin D ( CD ) have been implicated in the progression of several human tumors . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Relationship between cathepsin B , cathepsin D expression and biological behaviour in mucinous colorectal carcinoma . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Development of murine HA 1 hepatoma was accompanied by increased activity of cathepsin B ( in ascitic cells ) , cathepsin D ( in ascitic fluid ) and increased activity of procathepsin B . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In neither cell type did Ras expression increase the expression , processing or secretion of 2 other major lysosomal proteases , cathepsin B and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| There was , however , no significant difference between tumour budding and the activity of cathepsin D in tumour tissue , but we found a statistically significant difference between the activity of cathepsin B in the homogenate and cytosol of tumour tissue and budding positive tumours ( p=0 . 027 , p=0 . 004 , respectively ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Increased apoptosis was prevented by specific inhibition of the caspase cascade using the broad spectrum caspase inhibitor z VAD fmk or a caspase 3 inhibitor , or by using functional inhibitors for cathepsin D ( pepstatin A ) or cathepsin B . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The hK 2 activated thapsigargin prodrug was not activated by cathepsin B , cathepsin D , and urokinase but was an excellent substrate for plasmin . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Translocation of the lysosomal proteases cathepsin B and cathepsin D into the cytosol was observed after treatment with these drugs , and apoptosis was inhibited by pepstatin A , an inhibitor of cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , some tumor metastasis associated genes cathepsin B , cathepsin D , cathepsin L , MMP 2 / TIMP 2 were also upregulated by CT 120 , upon which CT 120 might be involved in tumor invasiveness and metastasis . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| After centrifugation , the contents of protein , haemoglobin , vitamin A , vitamin E , vitamin C , as well as the activities of cathepsin B , cathepsin D , superoxide dismutase , catalase , glutathione peroxidase and reductase were assessed in the whole homogenate , its supernatant and sediment . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The enzymatic degradation studies of the polymeric conjugates were performed in the presence of collagenase type 4 ( Clostridiopeptidase A ; EC 3 . 4 . 24 . 3 ) , cathepsin B ( EC 3 . 4 . 22 . 1 ) , cathepsin D ( EC 3 . 4 . 23 . 5 ) and tritosomes . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Presumable order of events in this complicated cascade is that aspartyl protease ( cathepsin D ) activates cysteine proteases ( e . g . cathepsin B ) that can activate pro uPA . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| MATERIAL / METHODS : Blood for examinations was collected from 15 patients with abdominal aorta aneurysm without coexisting diseases , and from the same number of healthy subjects , to determine the activity of lactic acid dehydrogenase ( LDH ) , 5 ' nucleotidase ( 5 ' NT ) , gamma glutamyltranspeptidase ( GGTP ) and cholinesterase ( ChE ) , alanine aminotransferase ( ALT ) , aspartic aminotransferase ( AST ) , cathepsin B ( CB ) , cathepsin D ( CD ) , and cathepsin L ( CL ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| High pressure processing up to 500 MPa enhanced the activity of cathepsin B , H , and L , whereas the activity of cathepsin D increased up to 300 MPa and decreased above 300 MPa . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activity of cathepsin B was measured with Bz DL arginine pNA as a substrate at pH 6 . 0 , while cathepsin D activity was determined with urea denatured hemoglobin ( pH 4 . 0 ) . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The activities of the following glycosidases were measured : alpha glucosidase ( AGU ) , beta galactosidase ( BGA ) , alpha mannosidase ( AMAN ) , beta glucuronidase ( GCU ) , beta hexosaminidase ( HEX ) , and the following proteases : cathepsin B ( CATH B ) , dipeptidyl peptidase 1 ( DPP 1 ) , cathepsin H ( CATH H ) , dipeptidyl peptidase 2 ( DPP 2 ) , tripeptidyl peptidase 1 ( TPP 1 ) , and cathepsin D ( CATH D ) activity . ^^^ RESULTS : Activity of the glycosidases beta galactosidase , alpha mannosidase , beta glucuronidase and beta hexosaminidase , as well as of the peptidases cathepsin B , cathepsin D , dipeptidyl peptidase 1 , and tripeptidyl peptidase 1 , was elevated during the first 5 years of SS , and it increased further between 5 and 10 years after diagnosis . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In addition , alterations in expression of the lysosomal cysteine and aspartic proteases , cathepsin B ( CB ) and cathepsin D ( CD ) and the endogenous cysteine protease inhibitor cystatin C were observed utilizing immunocytochemical analysis at 24 , 48 , and 72 h following 6 OHDA exposure . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Function of liver activation regulated chemokine / CC chemokine ligand 20 is differently affected by cathepsin B and cathepsin D processing . ^^^ Whereas cathepsin D is secreted in the extracellular milieu , cathepsin B activity is confined to cytosol and cellular membranes . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In humans , TPP 1 constitutes an integral part of the lysosomal proteolytic apparatus , which , includes numerous hydrolytic enzymes , mostly cysteine proteases ( cathepsin B , C , H , K , L , and others ) , but also serine ( cathepsin A ) and aspartic ( cathepsin D ) proteases . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| These were designated cpsA , cpsB , cpsC , cpsD and cpsE and encoded proteins of > 130 , 243 , 230 , 246 and 455 amino acids , respectively , that showed homology with the genes of the cps cluster , involved in polysaccharide biosynthesis , in Streptococcus pneumoniae Type 19F . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Additionally , we demonstrated that CpsC is required for CpsD tyrosine phosphorylation and that CpsB influences dephosphorylation of CpsD . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In Streptococcus pneumoniae , CpsB , CpsC , and CpsD affect tyrosine phosphorylation and are critical for the production of a mature capsule in vitro . ^^^ Based on these results , we propose a model for capsule regulation where CpsB , CpsC , CpsD , and ATP form a stable complex that enhances capsule synthesis . . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| We have previously determined that CpsD is an autophosphorylating protein tyrosine kinase , demonstrated that CpsC is required for CpsD tyrosine phosphorylation , and shown that CpsB is required for dephosphorylation of CpsD . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| In Streptococcus pneumoniae , CpsB , CpsC , and CpsD are essential for encapsulation , and mutants containing deletions of cpsB , cpsC , or cpsD exhibit rough colony morphologies . ^^^ CpsD is an autophosphorylating protein tyrosine kinase , CpsC is required for CpsD tyrosine phosphorylation , and CpsB is a phosphotyrosine protein phosphatase . ^^^ Double mutants having a single tyrosine residue at position 2 , 3 , or 4 in the ( YGX ) ( 4 ) repeat domain and lacking CpsB exhibited a rough colony morphology , indicating that in the absence of an active protein tyrosine phosphatase , phosphorylation of just one of the tyrosine residues in the ( YGX ) ( 4 ) repeat was sufficient to inactivate CpsD . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| CpsA , CpsB , CpsC , and CpsD are part of a tyrosine phosphorylation regulatory system involved in modulation of capsule synthesis in Streptococcus pneumoniae and many other gram positive and gram negative bacteria . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The effect that mutations in the conserved capsular polysaccharide biosynthesis genes cpsA , cpsB , and cpsD have on virulence of Streptococcus pneumoniae . ^^^ In the present study , the impact that mutations in cpsA , cpsB , and cpsD have on CPS production and on virulence in mice infected via systemic and intranasal routes was investigated . ^^^ Strains exhibiting rough colony morphologies ( in which either the cpsB or cpsD gene had been deleted ) were avirulent , but a smooth , partially encapsulated strain ( in which the cpsA gene had been deleted ) was as virulent as the wild type strain . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| The CPS biosynthesis proteins CpsB , CpsC , and CpsD function to regulate CPS production via tyrosine phosphorylation of CpsD . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| Expression of CTSB , CTSD , CTSH , CTSK , CTSL , CTSS , and CTSZ genes was detected in the endometria of cyclic and early pregnant ewes with distinct temporal and spatial expression patterns . ^^^ In the d 18 and 20 conceptus , expression of CTSB , CTSD , CTSL , and CTSZ mRNA was detected in the trophectoderm . ^^^ |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P07858 |
Pubmed |
SVM Score :0.0 |
| NA |
|