| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| The influence was studied of different diets on the activity of cathepsin D ( PSCatD ) , pepstatin ( PIA ) and leupeptin ( LIA ) insensitive acid autolytic activity ( AAA ) , RNA , DNA and protein in skeletal leg muscle ( LM ) and liver of 37 mice . ^^^ In AAA , cathepsin D participates in 58 . 5 % and 50 . 5 % in the liver and LM inhibition , respectively , but leupeptin accounted for about 15 % and 27 % ( in the presence of Mg++ ) of inhibition . . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| To investigate the potential contribution of the lysosomal compartment in the processing of amyloid precursor protein ( APP ) to amyloid beta peptides ( A beta s ) , we stably overexpressed a series of lysosomal proteases ( the cysteine proteases , cathepsins B , L and S , and the aspartic protease , cathepsin D ) in a human kidney epithelial cell line ( 293 ) transfected to express high levels of beta APP . ^^^ A beta secretion was assessed by immunoprecipitation and ELISA and found to be increased approximately 2 fold following cathepsin S expression , but to be unchanged ( cathepsins B , L ) or decreased ( cathepsin D ) in the other double transfectants . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Candidate gamma secretases in the generation of the carboxyl terminus of the Alzheimer ' s disease beta A 4 amyloid : possible involvement of cathepsin D . beta A 4 amyloid peptide , the main constituent of amyloid plaques and cerebrovascular amyloid deposits associated with Alzheimer ' s disease , derives from a large precursor protein ( APP ) by the action of beta and gamma secretases , the unidentified endoproteases which release the amino and carboxyl termini of beta A 4 , respectively . ^^^ Thus , a cathepsin D like activity from human brain is a candidate for APP gamma secretase ( s ) . . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Analysis of the cleavage sites indicated that cathepsin D hydrolyzed the methionine aspartate bond generating the in vivo amino terminus of A beta . ^^^ Consequently , cathepsin D from both Alzheimer ' s diseased and control brains was compared to determine whether there were any differences which could account for an increase in A beta production in Alzheimer ' s disease . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate , for the first time , immunohistochemical evidence that A beta and cathepsin D , a lysosomal enzyme , accumulate in vacuolated rat soleus muscle due to chloroquine induced myopathy . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Plaque like lesions and amyloid angiopathy were investigated in the frontal cerebral cortex of four patients with hereditary cerebral hemorrhage with amyloidosis ( Dutch ) ( HCHWA D ) , using immunohistochemical [ antibodies to beta amyloid protein ( A beta ) , beta protein precursor ( beta PP ) , synaptophysin , ubiquitin ( UBQ ) , cathepsin D , paired helical filaments ( PHF ) and glial fibrillary acidic protein ( GFAP ) ] , enzymehistochemical ( acid phosphatase ) and silver [ methenamine silver ( MS ) and Palmgren ] staining methods . ^^^ Whereas A beta and MS positive diffuse plaques were found in all patients , only the three older patients showed neuritic or congophilic plaques , which were acid phosphatase and cathepsin D positive and contained beta PP , synaptophysin and UBQ positive , but PHF negative neurites . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| SNAP 25 , chromogranin A and cathepsin D also marked injured axons at all survival times studied ( 2 . 5 h 2 weeks ) , although they were not as sensitive or specific as beta APP . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Amyloidogenic processing of human amyloid precursor protein in hippocampal neurons devoid of cathepsin D . betaA 4 Amyloid peptide , the main component of the amyloid plaques in the brain of Alzheimer ' s disease patients is produced from amyloid precursor protein ( APP ) by proteolytical processing . ^^^ Here we tested this hypothesis using primary cultures of hippocampal neurons derived from cathepsin D deficient ( knock out ) mice and expressing wild type human APP and two clinical APP variants via recombinant Semliki Forest virus . ^^^ We demonstrate APP secretory processing , production of carboxyl terminal amyloid fragments , and secretion of the betaA 4 amyloid peptide in the complete absence of cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Amino terminal sequence analysis indicated the Abeta degrading proteinase was cathepsin D . ^^^ Purified cathepsin D hydrolyzed Abeta between Phe 19 and Phe 20 . ^^^ Cathepsin D is likely to be involved in the intracellular clearance of aggregatable Abeta , since Abeta fragments with Phe 20 at the amino terminus have been reported to be secreted from several lines of cultured cells . . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| A beta amyloid peptide variant related with familial Alzheimer ' s disease and hereditary cerebral hemorrhage with amyloidosis is poorly eliminated by cathepsin D . ^^^ Cathepsin D is possibly involved in the intracellular clearance of Abeta ( Hamazaki , H . ( 1996 ) FEBS Lett . , in press ) . ^^^ The present work shows that cathepsin D hydrolyzes wild type Abeta 20 times faster than a variant Abeta with a substitution at residue 21 from Ala to Gly . ^^^ Since the substitution has been linked to familial Alzheimer ' s disease and hereditary cerebral hemorrhage with amyloidosis ( Hendriks et al . ( 1992 ) Nature Genet . 1 , 218 221 ) , the present observations suggest that the inefficient elimination of Abeta by cathepsin D is capable of being one of causes of the amyloid fibril formation . . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| However , cathepsin D does not cleave peptides bearing mutations that were previously shown to drastically lower or fully block A beta secretion by transfected cells . ^^^ Furthermore , cathepsin D hydrolyses recombinant baculoviral delta NL beta APP 751 at a 6 fold higher rate than beta APP 751 and gives rise to a 12 kDa C terminal product that is recognized by antibodies fully specific of the N terminus of A beta . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| As cathepsin D was found to cleave the betaAPP 100 FLAG in the vicinity of the C terminus of the beta amyloid peptides and cathepsin B has a high carboxypeptidase activity at low pH , the possibility can not be excluded that cathepsins D and B are involved in the amyloidogenic processing of APP . . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| When roots of intact plants were treated with JA , high levels of proteinase inhibitor 2 ( pin 2 ) , cathepsin D inhibitor , leucine aminopeptidase and threonine deaminase mRNAs accumulated in the systemic leaves . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| In addition , mature and proenzyme forms of the proteases cathepsin B and cathepsin D , a candidate APP secretase , were identified in most early endosomes in Alzheimer brains but were detectable in only a minor proportion of endosomes in normal brain . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| To understand the retinal changes in Alzheimer disease ( AD ) patients , pathological and immunocytochemical studies were performed on retinal cells in the chloroquine treated rats at 0 , 4 , 8 , 12 , 16 , 20 , and 24 weeks after the initial injection , using anti amyloid precursor protein ( APP ) , amyloid beta protein ( A beta ) , apolipoprotein E ( apoE ) , ubiquitin , and cathepsin D antibodies . ^^^ Intense immunoreactivities for anti APP , A beta , apoE ( an associated protein ) , and ubiquitin co localized in the swollen ganglion cells and Muller cells by 20 24 weeks together with the lysosomal enzyme cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Immunoreactivity of cathepsin D was markedly increased in lysosomes filled with A beta fibrils . ^^^ These findings indicated that A beta 1 40 , A beta 1 42 , A beta 1 43 and longer A beta species were generated in the lysosomes of the transgenic pancreas , and suggested that the activation of cathepsin D , a candidate gamma secretase , leads to acceleration of A beta amyloid formation . . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Human beta secretase candidates , MP 78 ( h MP 78 , EC 3 . 4 . 24 . 15 ) and cathepsin D ( Cat D , EC 3 . 4 . 23 . 5 ) , were evaluated for their ability to enhance amyloid beta protein ( A beta ) secretion when overexpressed in beta APP containing cells . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| In this work , we used a sensitive in vitro method of detection to investigate the role of cathepsin D in the proteolytic processing of a 100 amino acid C terminal fragment ( C 100 ) inclusive of beta A 4 and cytoplasmic domain of APP . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Moreover , we observed no risk modifying interaction and no linkage disequilibrium between FE 65 and the gene encoding the acid protease cathepsin D ( catD ) , which like FE 65 is involved in APP metabolism and is also located on chromosome 11p15 . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| On the other hand , F ( ab ' ) ( 2 ) fragments formed by digesting the mAb by cathepsin D showed that the L chains were intact and the H chains were truncated . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| These structures were immunopositive for human amyloid precursor protein ( APP ) , human amyloid beta peptide ( Abeta ) , cathepsin D , apolipoprotein E and ubiquitin . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| The selective NMDA receptor antagonist D ( ) 2 amino 5 phosphonovalerate completely blocked internalization of Abeta , up regulation of cathepsin D , and activation of microglia . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| In vitro , the Abeta 34 site is processed specifically by BACE 1 and BACE 2 , but not by cathepsin D , a closely related aspartyl protease . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| Similar to what has been shown in Alzheimer ' s disease , cathepsin D , a lysosomal hydrolase , was redistributed to early endosomes in NPC Purkinje cells , where it co localized with C99 / APP CTF . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| At electron microscopy , Abeta , APP C terminal , cathepsin D , and flotillin 1 epitopes were found in the lumen of multivesicular bodies . ^^^ Multivesicular bodies could secondarily liberate their content in the extracellular space as suggested by the association of cathepsin D with Abeta peptide in the extracellular space . . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| The T allele of the CTSD genotype was associated with a 50 % decrease in Abeta ( 42 ) levels in the cerebrospinal fluid . ^^^ Thus , we demonstrate a significant impact of the CTSD genotype on Abeta ( 42 ) levels in the cerebrospinal fluid of AD patients and underpin the importance of the validation of susceptibility genes by examining their potential pathophysiological relevance . . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| The amount of plaque Abeta 40 was greater in patients carrying the CTSD T allele than in non carriers , and in patients bearing APOE e 4 allele compared with non carriers . ^^^ Possession of the CTSD T allele had no effect on plaque Abeta 42 ( 3 ) load or degree of CAA . ^^^ Possession of the CTSD T allele does not increase the risk of developing AD per se , but has a modulating effect on the pathogenesis of the disorder by increasing , in concert with the APOE e 4 allele , the amount of Abeta deposited as senile plaques in the brain in the form of Abeta40 . . ^^^ |
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| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| The data of both independent samples suggest that the CTSD rs 17571 polymorphism does not affect APP processing but shows significant effects on tau processing . ^^^ |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P05067 |
Pubmed |
SVM Score :0.0 |
| NA |
|