| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.50136909 |
| Affinity purified human plasma and synovial fluid fibronectin was digested with cathepsin D at pH 3 . 5 for 0 18 h and proteolysis stopped with pepstatin . 0.50136909^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Limited proteolytic cleavage of fibronectin and plasma cold insoluble globulin with cathepsin D produced two major fragments . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Predigestion of Fn with u PA increased the molecule ' s vulnerability to further attack by the enzymes plasmin and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The N terminal 70 kDa fragment of human plasma fibronectin , purified from a cathepsin D digest , is characterized by lack of stability . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Different results were obtained with a cathepsin D digest of fibronectin that exhibited gelatinolytic and laminolytic activities only after incubation with Ca++ . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Monocyte derived macrophage and alveolar macrophage fibronectin production and cathepsin D activity . ^^^ The secretion and regulation of cathepsin D ( important for the final breakdown of collagen ) and fibronectin ( involved in the healing process ) in human peripheral blood monocytes ( PBM ) and pulmonary alveolar macrophages ( PAM ) were investigated . ^^^ Thus , both macrophage cathepsin D activity and fibronectin secretion are increased by gamma interferon while macrophage cathepsin D activity , but not fibronectin secretion , is decreased by LPS . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Fibronectin contains two latent gelatinolytic enzymes , FN gelatinase and FN laminase that can be activated in the presence of Ca2+ from the purified cathepsin D produced 190 kDa fibronectin fragment . ^^^ These results have demonstrated that in contrast to cathepsin D , Achromobacter collagenase activates two matrix degrading proteinases from fibronectin , FN Gelatinase und FN Laminase . . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Two proteinases , FN gelatinase and FN laminase , were identified in cathepsin D fibronectin digest . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The purified 190 kDa fibronectin fragment produced by cathepsin D can be spontaneously activated in the presence of CaCl 2 . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Digestion of human plasma fibronectin with a combination of trypsin and cathepsin D produced six major fragments . ^^^ Cathepsin D digestion produced an 83K heparin binding , monoclonal antibody reactive fragment that contains the interchain disulfide bond ( s ) linking the two fibronectin chains at their C termini . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Cellular FN consisted of at least four subunits differing in size of the fragments generated by cleavage of the C terminal region with cathepsin D . ^^^ Among these , the two smaller subunits appeared to be closely similar to the major subunits of plasma FN , whereas the other subunits were more related to those of cellular FN in the size of cathepsin D cleaved C terminal fragments and in the reactivity with anti peptide antibodies . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| A time course digestion of fibronectin with cathepsin D produced a number of proteolytic fragments possessing DNA binding activity . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| FN saturated with sodium oleate was hydrolysed at a higher rate by trypsin , cathepsin D , thermolysin and pancreatic elastase than native FN . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Human plasma FN was cleaved with cathepsin D into three main fragments : 140 kDa and 70 kDa single chain and 140 kDa double chain polypeptides . ^^^ Neither FN , cathepsin D , nor any products from previous purification steps were proteolytically active under the conditions of the assay . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The mitogenic activity of a heparin binding fibronectin fragment ( Mr 35 , 000 ) produced by cathepsin D digestion . ^^^ Fibronectin was partially digested using the lysosomal proteinase cathepsin D and the fragments produced tested for their effect on the rate of DNA synthesis in quiescent Chinese Hamster Fibroblasts ( CH 23 ) . ^^^ A mitogenically active digest was produced after digestion of fibronectin with cathepsin D for 2 to 3h at a 1 : 200 ( w / w ) ratio of enzyme to substrate at 37 degrees C . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The isotype of fibronectin present in human adult and fetal lung tissues was studied by limited proteolysis by cathepsin D followed by immunoblot analysis with domain specific antibodies . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Monoclonals AB 3 and CPG 1 , which recognize epitopes within 40 , 000 daltons of the carboxy terminus of intact fibronectin , and the cathepsin D derived , disulfide linked fragments that contain these epitopes each inhibited the opsonic function of 180K opFnf . ^^^ Thus , the inhibitory effects produced by monoclonals AB 3 and CPG 1 and by cathepsin D derived , disulfide linked fragments containing their corresponding epitopes demonstrated that the opsonic domain within 180K opFnf is immunologically similar to regions within the carboxy terminus of intact plasma fibronectin . ( ABSTRACT TRUNCATED AT 400 WORDS ) . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Fibronectin fragments with differing biological activities were produced by proteolytic digestion with trypsin and cathepsin D and sequential affinity chromatography on gelatin agarose and heparin Sepharose . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Plasma fibronectin was cleaved into a number of biologically active fragments by trypsin and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The labeled fibronectin was then cleaved with cathepsin D under conditions that yield a large ( 137 , 000 dalton ) fragment that lacks collagen binding properties , and a smaller ( 72 , 000 dalton ) fragment that retains the ability of fibronectin to bind to collagen . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Stimulation of DNA synthesis by cathepsin D digests of fibronectin . ^^^ These findings led us to investigate the role of fibronectin fragmentation in the regulation of cell growth in vitro , and we report here that whilst fibronectin is without effect , its cathepsin D digests possess the ability to initiate DNA synthesis in serum deprived quiescent cultures of normal hamster fibroblasts . . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| A large cathepsin D derived fragment from the central part of the fibronectin subunit chains . ^^^ A mild cathepsin D digest of fibronectin only contained single chain peptides of 200 , 140 and 70 kDa and double chain fragments of about 300 and 140 kDa containing the C terminal disulfide link . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| A 70 kDa fragment obtained by cathepsin D treatment which comprises the N terminal part of the fibronectin chains , exhibited in 8M urea a spectrum , at 20 degrees C , identical to that of the native fragment and its thermal unfolding was shifted to lower temperatures by only 10 degrees C . ^^^ The central portions of the fibronectin chains were remarkably unfolded under the same conditions as clearly demonstrated by the spectra and transition profiles of a cathepsin D raised 125 / 140 kDa fragment which originates from this region . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Early and late cathepsin D derived fragments of fibronectin containing the C terminal interchain disulfide cross link . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Resolution of a cathepsin D digest of plasma fibronectin on heparin Sepharose yielded , in addition to non bound and weakly retained material ( Fraction 1 and 2 ) , various fragments which were not eluted until 0 . 25M NaCl ( Fraction 3 ) and 0 . 5M NaCl ( Fraction 4 ) was applied . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The acid protease cathepsin D generated fragments of 31 33 . 5 kDa from the COOH terminal heparin binding domain of Fn which possessed high immunoreactivity with anti CS 1 . ^^^ Although the digestion of Fn with neutral proteases ( neutrophil elastase , cathepsin G , chymotrypsin , trypsin ) generated fragments from the COOH terminal heparin binding domain of similar molecular weight as with cathepsin D , the exposure of CS 1 did not occur . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Matrix metalloproteinase expression in human breast cancer : an immunohistochemical study including correlation with cathepsin D , type 4 collagen , laminin , fibronectin , EGFR , c erbB 2 oncoprotein , p 53 , steroid receptors status and proliferative indices . ^^^ The current study aimed to investigate the immunohistochemical expression of matrix metalloproteinase 3 ( MMP 3 , stromelysin 1 ) in correlation with the expression of Basement Membrane ( BM ) antigen ( type 4 collagen , laminin ) , fibronectin , cathepsin D , p 53 , c erbB 2 , proliferative activity ( Ki 67 , PCNA ) , steroid receptor content as well as to the other conventional clinicopathological parameters in breast cancer . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The immunohistochemical expression of CD 44 in a series of 110 colorectal carcinomas and 25 adenomas was examined using the monoclonal mouse anti human phagocytic glycoprotein 1 , CD 44 ( clone DF 1485 ) in correlation with the expression of basement membrane ( BM ) antigens ( type 4 collagen , laminin ) , fibronectin , cathepsin D , p 53 , Rb , bcl 2 , c erbB 2 , EGFR , proliferation indices ( Ki 67 , PCNA ) and with other conventional clinicopathological variables . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Native fibronectin was digested with cathepsin D to obtain fragments similar to those of serum fibronectin . ^^^ The capacity of fibronectin to bind IgA was examined with a mixture of purified IgA 1 and cathepsin D digested fibronectin fragments . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Both enzymes can be generated and activated in the presence of Ca2+ from the purified cathepsin D produced 190 kDa fibronectin fragment . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| The MT expression was studied in comparison with the expression of the basement membrane ( BM ) antigens ( type 4 collagen , laminin ) , fibronectin , cathepsin D , adhesion molecule CD 44 , p 53 protein , the pRb , c erbB 2 oncoprotein , EGFR , stromelysin 1 , proliferation indices ( Ki 67 , PCNA ) , steroid receptor content as well as with other conventional clinicopathological parameters of breast cancer . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| In tumor tissue specimens of 27 primary and 17 secondary glioblastomas and the precursor lesions , the immunohistochemical expression patterns of the membrane protein CD44s , the basal lamina proteins laminin , collagen 4 , and fibronectin , the lectin galectin 3 recognizing tenascin and N CAM as well as of the matrix degrading enzymes matrix metalloproteinase MMP 2 and MMP 9 , and cathepsin D were studied . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Secretion of extracellular matrix ( fibronectin ) , growth factor ( transforming growth factor beta ) and protease ( cathepsin D ) by hepatoma cells . ^^^ Based on immunoblotting , PLC / PRF / 5 cells secrete fibronectin ( an extracellular matrix constituent ) , transforming growth factor beta ( TGFbeta ; a growth factor ) , and cathepsin D ( a protease ) . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| In addition , mRNA for the Ang 2 generating proteases cathepsin D and ACE was increased by FN . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of the extracellular matrix protein tenascin in laryngeal epithelial lesions : correlation with fibronectin , CD 44 , cathepsin D and proliferation indices . ^^^ TN expression was correlated with the expression of fibronectin , CD 44 and cathepsin D ( CD ) proteins , with the proliferation indices Ki 67 and proliferating cell nuclear antigen ( PCNA ) as well as with conventional clinicopathological variables . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Forty five gliomas ( astrocytomas , glioblastomas , oligodendrogliomas , and mixed gliomas ) were investigated for the immunohistochemical expression of the membrane protein CD44s , the basal lamina proteins laminin , collagen 4 , and fibronectin , the lectin galectin 3 recognizing tenascin and N CAM , as well as for the matrix degrading enzymes metalloproteinases MMP 2 , MMP 9 , and cathepsin D . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| We show that HIF 1 regulates the expression of genes encoding cathepsin D ; matrix metalloproteinase 2 ; urokinase plasminogen activator receptor ( uPAR ) ; fibronectin 1 ; keratins 14 , 18 , and 19 ; vimentin ; transforming growth factor alpha ; and autocrine motility factor , which are proteins that play established roles in the pathophysiology of invasion . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Expression of mRNAs for Ang 2 generating proteases cathepsin D , cathepsin G , ACE , and chymase was increased by FN . ^^^ Expressions of cathepsin D and cathepsin G proteins were also increased by FN . ^^^ Antisense oligodeoxynucleotides ( ODNs ) that hybridize with cathepsin D and cathepsin G significantly inhibited FN increased Ang 2 in conditioned medium and cell extracts . ^^^ Ang 2 type 1 receptor antagonist CV 11974 completely , and antisense cathepsin D and cathepsin G ODNs partially inhibited the FN stimulated growth of human VSMC . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Fraction ( F ) 2 and FIII obtained by heparin Sepharose after digestion of partially purified fibronectin ( FN ) with cathepsin D and F 3 , obtained like FIII but from untreated FN , exerted activity ( arFN ) on unfolded purified von Willebrand factor ( vWF ) that controls vWF multimer size . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| We identified 145 differentially secreted proteins ( > 1 . 5 fold change ) , several of which were previously reported as either up regulated ( e . g . cathepsin D , macrophage colony stimulation factor , and fibronectin receptor ) or down regulated ( e . g . profilin 1 and IGFBP 7 ) proteins in pancreatic cancer , confirming the validity of our approach . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| Collapse of statistical indices was defined as any of the following : pattern deviation probability plot without a single VF location showing P < 0 . 5 % ; corrected pattern standard deviation ( CPSD ) and pattern standard deviation ( PSD ) probability less than 5 % or within normal limits ( WNL ) ; short term fluctuation ( SF ) probability WNL ; glaucoma hemifield test ( GHT ) not outside normal limits ( ONL ) ; or presence of a low patient reliability comment triggered by 40 % or more false negative ( FN ) responses . ^^^ |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P07339 and P02751 |
Pubmed |
SVM Score :0.0 |
| NA |
|