Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.80917312
Full length NF L had only a relatively weak interaction with another full length NF L molecule , but reacted more robustly with full length NF M or NF H lacking only part of the head domain . 0.80917312^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.71215609
These data extend earlier cell free demonstrations that NF H preferentially associates with NF L rather than alpha IN . . 0.71215609^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.54323919
In order to study the role of NF H in a neurofilament network formation in neurons , we coexpressed NF H with neurofilament protein L ( NF L ) in Sf 9 cells using the baculovirus expression system . 0.54323919^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.54838017
As obligate heteropolymers , NF assembly requires interactions between the core NF L with NF M or NF H subunits , a process inhibited by NF head domain phosphorylation . 0.54838017^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.7118323
By monitoring the activity of the lacZ reporter gene product , we are able to show that the interactions of NFL with NFL , NFM , or NFH are stronger than the interactions of NFM with NFM or NFH and the interaction of NFH with NFH . 0.7118323^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
When cultures were exposed to okadaic acid for 0 . 5 hrs and harvested in buffer containing Triton 10 100 , NF H was nearly completely redistributed to the detergent soluble fraction while NF M and NF L required a longer exposure to the drug before undergoing a similar shift . ^^^ Upon removal of okadaic acid from the culture medium following a 0 . 5 hr treatment , NF L and NF M returned to the Triton 10 100 insoluble fraction within 2 hrs while NF H required 10 hrs for recovery . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Concentrations of seven structural proteins MAP 2 , tau , NF H , NF M ( 150 kDa ) , NF L ( 68 kDa ) , tubulin and spectrin were measured in rat brain regions by immunoblot methods . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We found 30 50 % lower levels of three NF proteins , NF 200 ( NF H ) , NF 160 ( NF M ) , and NF 68 ( NF L ) , in the VTA of LEW compared to F 344 rats by use of immunolabeling and Coomassie blue staining . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Immunocytochemically , the uninduced F 9 cells expressed several neuronal antigens including the 68 kDa neurofilament protein , the 200 kDa neurofilament protein , neural cell adhesion molecule ( N CAM ) and a neuronal specific tubulin isoform ( TUJI ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Quantitative northern blotting was used to examine changes in the levels of mRNAs for the low , middle and high molecular weight neurofilament proteins ( NF L , NF M , NF H ) as well as peripherin , vimentin and glial fibrillary acidic protein ( GFAP ) . ^^^ Increases in NF H mRNA levels were somewhat delayed relative to those of NF L and NF M . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We have cloned and examined the 5 ' flanking regions of the heavy ( NF H ) , light ( NF L ) and mid sized ( NF M ) mouse neurofilament ( NF ) genes in order to begin to characterize the regions of each gene that regulate NF transcription . ^^^ Constructs bearing upstream regions to 4000 in NF H , to 5600 in NF L and to 4500 in NF M were expressed at low levels in neuronal and in non neuronal cells . ^^^ Progressive deletion of 5 ' flanking sequence to 385 in NF H , to 325 in NF L and to 505 in NF M caused a several fold increase of transcription from the transfected plasmids . ^^^ Negative upstream regions are located between 1314 and 385 in NF H , between 936 and 325 in NF L and between 874 and 505 in NF M . ^^^ Additional negative regions are present further upstream in NF L and in NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Incubation of intact filaments with PDPK led to strong labeling of the heavy ( NF H ) and middle ( NF M ) molecular weight NF proteins and weaker labeling of the low molecular weight protein ( NF L ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The immunoblots showed negligible or trace amounts of NF L ( light ) and NF H ( heavy ) , while an NF M ( medium ) protein having a molecular mass ( Mr ) of 160 kD was present in abundance . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Monoclonal antibodies specific for the rod regions of each of the three NF subunits , or for phosphate dependent and independent antigenic sites in the tail region of the high ( NF H ) and middle ( NF M ) molecular weight ( Mr ) NF subunits as well as other MAbs to the extreme COOH terminus of NF L and NF M or the head region of NF M labeled a variable number of cortical LBs . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Chronic , but not acute , administration of either morphine or cocaine was found to decrease levels of the three NF proteins , NF 200 ( NF H ) , NF 160 ( NF M ) , and NF 68 ( NF L ) , by between 15 % and 50 % in the VTA by back phosphorylation , immunolabeling , and Coomassie blue staining . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Both immunoblotting and immunocytochemistry with redundant antibodies demonstrated the presence of neurofilaments ( NF H , NF M , NF L ) , but not glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Studies presented here test the expression system with neurofilament subunits , NF L and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
When NFs were reassembled from triplet proteins , NF L , NF M and NF H , they were oriented randomly and often fragmented , but their elongated filaments ( 12 15 nm wide ) and the cross bridges ( 4 5 nm wide ) connecting them were similar in appearance to those of isolated bovine NFs or in vivo rat NFs . ^^^ In contrast , when NFs were reconstituted from NF H alone or NF L + NF H , both had conspicuous projections and cross bridges , similar to those of triplet NFs . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Stable binding in the NF H and M proteins was 4 to 6 fold higher than in the NF L protein at all 2 , 5 HD concentrations , with specific activities of approximately 6 . 9 , 4 . 7 , and 1 . 3 mol / mol protein , respectively , at 212 . 5 mM . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Following SDS polyacrylamide gel electrophoresis , these proteins were identified as the 200 , 150 and 68 kD subunits of neurofilaments ( NF H , NF M and NF L , respectively ) , the 66 kDa associated protein , the 57 kDa intermediate filament like protein and the 52 kDa glial fibrillary acidic protein ( GFAP ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
This study sought to determine the extent to which each neurofilament subunit [ low ( NF L ) , mid ( NF M ) , or high ( NF H ) ] was present in Lewy bodies by using light , confocal , and electron microscopy . ^^^ The epitopes recognized by these antibodies included those in the NF L rod and tail domains ; the NF M head , rod , and tail domains , as well as epitopes within , and flanking , the multiphosphorylation repeat site ; and the NF H rod domain and multiphosphorylation repeat sites . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Furthermore , immunological analysis reveals that the rod domains of NF H , NF M and NF L are buried within the reassembled NF , whereas the head domain of NF M and the tail domains of all 3 NF proteins remain exposed following reassembly . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In contra distinction , the high Mr NF subunit ( NF H ) alone and in combination with NF M or NF L underwent reassembly to a lesser extent , i . e . 62 88 % of these proteins reassembled within 90 min . ^^^ Specifically , antibodies specific for epitopes in the rod domains of NF H , NF M and NF L failed to bind heteropolymeric filaments but recognized rod domains in the homopolymers . ^^^ Our results also showed that only copolymeric filaments of NF L and NF M , but not NF L / NF H and NF M / NF H were able to form long and stable 10 nm wide filaments . ^^^ In particular , they support the notion that only NF L / NF M , but not NF L / NF H or NF M / NF H might assemble into a stable filamentous network in vivo . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We carried out immunolabeling studies of purified bovine spinal cord neurofilaments ( NFs ) and filaments reconstituted from several combinations of the NF triplet polypeptides , NF H , NF M , and NF L . ^^^ One possible explanation is that , in native NFs , the NF H and NF M containing sidearms curtailed antibody access to NF L . ^^^ Taken collectively , and in combination with published structural information , our data are consistent with a subunit packing scheme in which an NF L containing dimer serves as the fundamental building block of most mammalian NFs , such that their sidearms consist of pairs of NF H / NF L , NF M / NF L , or NF L / NF L tailpiece extensions . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
All three neurofilament proteins , the high , middle , and low molecular proteins ( NF H , NF M , and NF L ) , were phosphorylated when intact filaments were incubated with the kinase . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The expression of neurofilament ( NF ) proteins was examined during postnatal development of the rat nervous system in order to elucidate the nature of NF expression during the period of transition from the embryonic ( immature ) to the adult ( mature ) stages of NF expression . mRNAs to the light ( NF L ) , mid sized ( NF M ) , and heavy ( NF H ) NF proteins were compared by Northern blots and by in situ hybridizations , in NF rich ( i . e . , DRG , spinal cord and brainstem ) and in NF poor ( i . e . , cerebellum and cerebral cortex ) regions of the developing rat nervous system . ^^^ In each tissue , the expression of NF H was delayed compared to that of NF L and NF M , as previously reported . ^^^ The present study now shows that the delayed expression of NF H is accompanied by parallel up regulations in the expressions of NF L and NF M , both at the levels of mRNA and protein . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We studied the effects of aluminum salts on the degradation of human neurofilament subunits ( NF H , NF M , and NF L , the high , middle , and low molecular weight subunits , respectively ) and other cytoskeletal proteins using calcium activated neutral proteinase ( calpain ) purified from human brain . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The mRNA levels of the low ( NF L ) and middle ( NF M ) sized NF subunits were significantly decreased as early as 1 day postaxotomy but the mRNA level of the large NF subunit ( NF H ) did not change until 7 days after axotomy . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments are composed of 3 polypeptides designated NF H , NF M , and NF L , all of which are subject to posttranslational phosphorylation . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
A neurofilament enriched preparation from bovine spinal cord contains endogenous protein kinases that phosphorylate high , middle , and low molecular weight neurofilament subunits ( NF H , NF M , and NF L ) , as well as certain other endogenous and exogenous substrates . 2 . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Isolated individual components of the triplet of neurofilaments from bovine brain can reassemble to make filaments with a specific structure , contrary to the already reported result that NF H and NF M can not make filaments alone but can only make filaments by co polymerization with NF L . 2 . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
NF H was demonstrated in 15 of 17 , NF M in six of 17 and NF L in one of 17 tumors ; GFAP was positive in nine of 17 patients . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Addition of NF M or NF H to NF L did not change the assembly properties of neurofilaments . ^^^ Unraveled filaments reconstituted from NF L plus either NF M or NF H indicated that NF M and NF H are incorporated evenly into each protofibril . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Left sciatic nerves were transected at postnatal day 3 ( P 3 ) , 6 ( P 6 ) , 8 ( P 8 ) , or 10 ( P 10 ) . mRNA and protein levels of the light ( NF L ) , mid sized ( NF M ) , and heavy ( NF H ) NF proteins were compared in L 4 and L 5 DRGs from the transected ( left ) vs . control ( right ) sides of the same animals at varying intervals after transection . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Cleavage at cysteine and chymotrypsin digestion were applied to two human neurofilament ( NF ) subunits , low and high molecular weight NF ( NF L and NF H ) , to locate the regions reacting with Bodian ' s silver stain and with several monoclonal antibodies , including NF specific antibodies and one that recognizes all intermediate filaments ( anti IFA ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Both antibodies bind to the 200 kilodalton ( kd ) ( NF H ) and 160 kd ( NF M ) but not to the 68 kd ( NF L ) NF triplet proteins . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Electrophoretically purified NF H ( 200 kDa ) , NF M ( 160 kDa ) , and NF L ( 68 kDa ) of human origin contained 24 , 18 , and 4 mol phosphate / mol protein , whereas bovine NF contained 53 , 23 , and 5 mol phosphate / mol protein , respectively . ^^^ Incubation of NF preparations with E . coli alkaline phosphatase removed about 55 % of the phosphate from NF H , about 30 % of the phosphate from both human and bovine NF M , but did not change the phosphate content of NF L . ^^^ Measured densitometrically at 615 nm , the staining intensity ( relative units / mol protein ) was 9 , 9 , and 3 for human and 10 , 13 , and 6 for bovine NF H , NF M , and NF L , respectively . ^^^ After phosphatase treatment , NF H , with half of its phosphate residues remaining , no longer formed blue complex with `` Stains all , ' ' the staining intensity of NF M decreased by 20 40 % , and the staining of NF L was not changed . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The low ( NF L ) and middle ( NF M ) molecular weight ( Mr ) neurofilament ( NF ) subunits are expressed before the high ( NF H ) Mr NF subunit in embryonic neurons . ^^^ By flow cytometry , more than 90 % of the D 283 cells expressed NF H and NF M in different states of phosphorylation , and an antiserum specific for the carboxy terminus of NF L labeled more than 60 % of these cells . ^^^ However , NF H , NF M , and NF L were expressed throughout the cell cycle in dual parameter studies of D 283 cells labeled with an antibody and propidium iodide . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The pattern of expression of NF isoforms in PNETs was reminiscent of that seen during normal mammalian development , such that phosphorylated NF H was only present in combination with NF M and NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Our findings indicate that epitopes located in the alpha helical core domains of NF H , NF M , and NF L are distinct from each other , yet are strongly conserved among the different species . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
These cDNA clones were used as probes to screen mouse genomic libraries , and cosmid clones containing both NF L and NF M sequences were isolated as well as overlapping cosmids containing the NF H gene . ^^^ The NF L and NF M mRNAs are detected early in the embryonal brain , with a progressive increase in their levels during development , while the NF H mRNA is barely detectable at embryonal stages and accumulates later in the postnatal brain . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
A new panel of greater than 300 monoclonal antibodies ( mAbs ) was prepared to the high , middle , and low Mr rat neurofilament ( NF ) subunits ( NF H , NF M and NF L , respectively ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Monoclonal antibodies ( mAbs ) to rat neurofilament ( NF ) proteins NF L , NF M , and NF H were used to examine the developmental programs of NF expression in rat embryos . ^^^ Unequivocal staining for NF H first appeared at E 15 , a time at which NF L and NF M had already attained their adult patterns of immunocytochemical staining . ^^^ Quantities of immunoreactive NF H increased very slowly and remained well below those of NF M and NF L for several weeks beyond birth . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In this report , we show that the three neurofilament subunits , NF L , NF M , and NF H , are not induced coordinately . ^^^ NF H accumulated only after longer term NGF treatment than required for NF L and NF M . ^^^ While NGF treatment resulted in 12 and 14 fold increases in NF L and NF M mRNA levels , respectively , over a 14 day period , no increase in the level of NF H mRNA was observed . ^^^ Analysis of the effect of NGF on the transcription of neurofilament genes showed 4 and 5 fold increases in the rates of NF L and NF M gene transcription , respectively , and no increase in the rate of NF H gene transcription . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The control of neurofilament ( NF ) protein gene expression was studied by determining and comparing the levels of mRNA to the heavy ( NF H ) , mid sized ( NF M ) and light ( NF L ) NF protein subunits in rat dorsal root ganglia ( DRG ) following sciatic nerve transection . mRNA to NF H ( 4 . 5 kb ) , to NF M ( 3 . 4 kb ) and to NF L ( 2 . 5 and 4 . 0 kb ) were identified in Northern blots and quantitated in dot blot analyses , using specific cDNA probes for each NF protein . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The human NF H gene has three introns , two of which interrupt the protein coding sequence at identical points to introns in the genes for the two smaller NF proteins , NF M and NF L . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
NF L , NF M and NF H , were purified by DE 52 and Mono Q anion exchange chromatographies in the presence of 6 M urea and were assembled in various combinations into filaments . ^^^ While prominent projections were not observed in the filaments assembled from NF L alone , reconstructed filaments containing NF L plus either NF M or NF H revealed many projections . ^^^ The average length of the projections in the filaments reconstructed from NF L and NF H was about 63 nm . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
NFs were probed biochemically and histochemically , using a large and well characterized library of monoclonal antibodies that included antibodies that are monospecific for each of the rat NF protein subunits ( NF H , NF M , and NF L ) as well as antibodies that recognized differential phosphorylated states of rat NF H and NF M . ^^^ Biochemical analyses of IDPN treated animals revealed enrichment of NF H , NF M , and NF L in homogenates of dorsal root ganglia and of proximal motor and sensory nerve roots as well as depletion of the three subunits in distal nerve roots and in sciatic nerves . ^^^ Immunoblot revealed a uniform enrichment of NF H , NF M , and NF L in NF aggregates as well as the same admixture of phosphorylated and dephosphorylated epitopes of NF H and NF M in experimental and in control tissues . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Exhaustive in vitro dephosphorylation of porcine neurofilaments ( NFs ) by alkaline or acid phosphatase did not cause a dissociation of the 210 kD ( NF H ) , 160 kD ( NF M ) , or 70 kD ( NF L ) subunits and had no effect on the reassembly of NFs from urea or guanidine solution . ^^^ Chemical phosphate analysis showed that as isolated NF H , NF M , and NF L contained about 22 , 11 , and 3 mol phosphate / mol polypeptide , respectively . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
A polyclonal antibody against the high molecular weight neurofilament polypeptide ( NF H ) obtained from cytoskeletal extracts of bovine spinal cord reacted with NF H , with the middle molecular weight neurofilament polypeptide ( NF M ) and with a M ( r ) 51 , 000 polypeptide , but not with the low molecular weight neurofilament polypeptide ( NF L ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The NR 4 antibody was used to target the light neurofilament subunit ( NF L ) , and the SMI 32 antibody was used to target the heavy neurofilament subunit ( NF H ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The studies show that the light ( NF L ) , mid sized ( NF M ) and heavy ( NF H ) NF mRNAs are lost at 8 and 16 h in primary cultures from postnatal day 2 ( P 2 ) rats , but much less so in cultures from postnatal day 16 ( P 16 ) and day 30 ( P 30 ) rats . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Distal to an orbital nerve crush , the neurofilament proteins NF L , NF M , NF H , and XNIF disappear from degenerating fibers . ^^^ In the aberrant retinoretinal projection , levels of XNIF , NF L , and M remained lower than in normally regenerating axons , whereas NF H and a phosphorylated form of NF M were undetectable for at least 35 d after nerve crush . ^^^ In tecta ablated frogs , staining of phosphorylation independent epitopes of XNIF , NF L , and M increased normally after axons entered the tract , but that of NF H and phosphorylated NF M remained low for at least 42 d after axotomy . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The transcription of the beta 2 , beta 4 and alpha 1 tubulin genes as well as that of the three neurofilament genes , NF L , NF M and NF H , was examined during the course of postnatal brain development . ^^^ NF L and NF H genes showed similar patterns of transcriptional change during the postnatal interval , with maximal rates of transcription at P 5 followed by a decline at later times . ^^^ The steady state levels of NF L and NF H mRNAs changed in a manner opposite to that of transcription and increased progressively during the postnatal interval . ^^^ This suggests that mRNA stabilization is the main factor regulating the steady state levels of NF L and NF H mRNAs in postnatal brain . ^^^ For the NF M gene , the developmental transcription pattern was also dissociated from steady state mRNA level changes , but differed from the transcription patterns of the NF L and NF H genes . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
LB derived protein at approximately 70 kDa did not contain epitopes detected by monoclonal antibodies to NF L , tau or the COOH termini of the NF H and NF M . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
An immunohistochemical survey was carried out on frozen sections of the early embryonic chick brain between 1 and 6 days of incubation , with antisera to the three neurofilament proteins ( NF L , NF M , NF H ) . ^^^ NF L and NF H could not be detected . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The expression of neurofilament proteins ( NF H , NF M , and NF L ) in replicating neuroepithelial cells and postmitotic neuroblasts in the embryonic chick trunk neural tube was examined by immunohistochemistry . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Following a 15 min pulse radiolabeling , NF H isoforms migrating from approximately 160 200 kDa , NF M isoforms migrating from approximately 97 k 145 Da , and a single 70 kDa NF L isoform were readily detectable within Triton soluble fractions from both undifferentiated and differentiated cells . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Intracellular delivery of antisera specific for the low ( NF L ) , middle ( NF M ) , or extensively phosphorylated high ( NF H ) molecular weight subunits did not prevent initial neurite elaboration , nor did it induce retraction of existing neurites elaborated by cells that had been previously treated for 1 d with dbcAMP . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
This study investigated changes in levels of mRNAs encoding the three neurofilament ( NF ) proteins NF L ( low ) , NF M ( medium ) , and NF H ( high ) and two growth associated proteins , GAP 43 and T alpha 1 alpha tubulin , in lumbar dorsal root ganglia of control and streptozocin induced diabetic rats . ^^^ The filters were probed sequentially with 32P labeled cDNAs encoding NF L , NF M , NF H , GAP 43 , T alpha 1 alpha tubulin , and citrate synthase . ^^^ In diabetes , NF L mRNA levels ( 2 . 5 and 4 kilobase [ kb ] transcripts ) were decreased by 35 ( P = 0 . 002 ) and 34 % ( P < 0 . 001 ) , respectively , the NF H mRNA level was decreased by 65 % ( P < 0 . 001 ) , but the NF M mRNA remained unchanged . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
These antibodies were specific for nonphosphorylated epitopes of the two low molecular weight Xenopus neurofilament proteins ( Xenopus NF L and the Xenopus neuronal intermediate filament protein , XNIF ) , both phosphorylated and nonphosphorylated epitopes of the Xenopus middle molecular weight neurofilament protein ( NF M ) , and a nonphosphorylated epitope of the Xenopus high molecular weight neurofilament protein ( NF H ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments , the neuron specific intermediate filaments , are composed of three immunochemically distinct subunits : NF L , NF M and NF H that can be either phosphorylated or unphosphorylated . ^^^ These rings were detected with anti NF L , NF M and dephosphorylated NF H / M antibodies , but not with anti phosphorylation independent NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
All phosphoisoforms of NF M as well as the low ( NF L ) and high ( NF H ) molecular weight NF proteins and alpha internexin were expressed in the somatodendritic domain of Purkinje cells and dentate nucleus neurons from about 20 weeks GA with a gradual compartmentalization of highly phosphorylated forms of NF M and NF H into axons by the end of gestation . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Likewise , the ability of NF 66 to polymerize with the low ( NF L ) , middle ( NF M ) , and high ( NF H ) M ( r ) NF proteins has not been defined . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Evidence is presented for the existence of a soluble heterotetramer containing the low and middle molecular weight neurofilament ( NF ) proteins , NF L and NF M , and one containing the low and high molecular weight proteins , NF L and NF H , and for their role in filament assembly . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Undifferentiated RN46A cells express low levels of neuron specific enolase ( NSE ) and low ( NF L ) and medium ( NF M ) but not high ( NF H ) molecular weight neurofilament proteins . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Levels of mRNA coding for neurofilament protein subunits NF L , NF M , and NF H have been determined by Northern blot analysis using 32P labeled cDNA probes . ^^^ The expression of NF L or NF H mRNA is not affected by acrylamide . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We dephosphorylated NF H using Escherichia coli alkaline phosphatase , then reassembled it into neurofilaments with NF M and NF L , and into NF H filaments with NF H alone . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Phosphorylation of native and reassembled neurofilaments composed of NF L , NF M , and NF H by the catalytic subunit of cAMP dependent protein kinase . ^^^ The effects of phosphorylation by A kinase on native neurofilaments ( NF ) composed of three distinct subunits : NF L , NF M , and NF H , however , have not yet been described . ^^^ In the native NF , A kinase phosphorylated each NF subunit with stoichiometries of 4 mol / mol for NF L , 6 mol / mol for NF M , and 4 mol / mol for NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Co incubation of purified high ( NF H ) and middle ( NF M ) but not low ( NF L ) molecular weight NF subunits prevents this AlCl 3 induced alteration in electrophoretic migration . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NFs ) are composed of triplet proteins , NF H , NF M , and NF L . ^^^ To understand the dynamics of NFs in vivo , we studied the dynamics of NF H and compared them to those of NF L , using the combination of microinjection technique and fluorescence recovery after photobleaching . ^^^ Thus , it was suggested that NF H is the dynamic component of the NF array and is interchangeable , but that it assembles with the other neurofilament triplet proteins in a more exchangeable way , implying that the location of NF H is in the periphery of the core NF array mainly composed of NF L subunits . ^^^ Immunoelectron microscopy investigations of the incorporation sites of NF H labeled with biotin compounds also revealed the lateral insertion of NF H subunits into the preexisting NF array , taking after the pattern seen in the case of NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Triton soluble , extensively phosphorylated NF H from NB2a / d1 cells was also capable of co assembling with purified NF L . ^^^ Following overnight dialysis to remove urea , high speed centrifugation sedimented both NF L and Triton soluble NF H from NB2a / d1 cells , demonstrating that Triton soluble NF H variants are assembly competent . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments , assembled from NF L , NF M , and NF H subunits , are the most abundant structural elements in myelinated axons . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Changes in neurofilament protein NF L and NF H immunoreactivity following kainic acid induced seizures . ^^^ In this study , neurofilament ( NF ) proteins were examined following intraperitoneal injection of kainic acid , and special reference was given to temporal changes in quantity and quality of the NF light ( NF L ) and heavy ( NF H ) subunits . ^^^ Decreases in NF L and phosphorylated NF H contents in the limbic structure at 3 days were correlated with the increasing kainic acid doses from 2 . 5 to 10 mg / kg . ^^^ NF L and phosphorylated NF H contents secondarily increased from 9 days onward , with approximately 20 % above the control level of phosphorylated NF H immunoreactivity at 27 days in the amygdala / pyriform cortex and ventral hippocampus . ^^^ Furthermore , the temporal changes of NF L and phosphorylated NF H contents were positively correlated with those of the neuronal cell adhesion molecule , a neuritic growth cone marker , substantiating postlesion regenerative reactions of NF proteins . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Dephosphorylated rat NF protein NF H was strongly phosphorylated by the purified enzyme ; NF proteins NF M and native NF H , but not NF L , were slightly phosphorylated . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Mammalian neurofilaments are composed of three polypeptide subunits , designed as NF L , NF M , and NF H , all of which are phosphorylated . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We report here on the in vivo assembly of alpha internexin , a type 4 neuronal intermediate filament protein , in transfected cultured cells , comparing its assembly properties with those of the neurofilament triplet proteins ( NF L , NF M , and NF H ) . ^^^ NF L coassembled with either NF M or NF H into filamentous structures in the transfected cells , but NF M could not form filaments with NF H . alpha internexin could coassemble with each of the neurofilament triplet proteins in the transfected cells to form filaments . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NFs ) , composed of three distinct subunits NF L , NF M , and NF H , are neuron specific intermediate filaments present in most mature neurons . ^^^ The competence of NF L to assemble into filaments is fully restored by coexpression of NF M or NF H to a level approximately 10 % of that of NF L . ^^^ Deletion of the head or tail domain of NF M or substitution of the NF H tail onto an NF L subunit reveals that restoration of in vivo NF L assembly competence requires an interaction provided by the NF M or NF H head domains . ^^^ We conclude that , contrary to the expectation drawn from earlier in vitro assembly studies , NF L is not sufficient to assemble an extended filament network in an in vivo context and that neurofilaments are obligate heteropolymers requiring NF L and NF M or NF H . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
CKI from rabbit reticulocytes phosphorylated all three NF subunits ( NF H , NF M and NF L ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Accordingly , in the present communication , we employed antibodies to detect at the light microscopic level the 68 kD Nf L and 170 200 kD Nf H neurofilament subunits in head injured patients who survived the traumatic event for periods ranging from 6 h to 59 days . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NF ) are composed of three polypeptides of differing molecular size , termed NF L , NF M and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
To investigate the mechanisms regulating neurofilament gene expression , we generated transgenic mice with high copy number of the intact human neurofilament light ( NF L ) and heavy ( NF H ) genes . ^^^ In contrast to the situation with NF L , the CNS of transgenic mice bearing multiple copies of the human NF H gene showed comparable increases in the levels of NF H mRNA and proteins . ^^^ These results suggest that the NF L and NF H genes are subject to different posttranscriptional regulation in the CNS . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The present study examined degenerating retinal terminals by means of immunohistochemistry and antibodies specific for the triplet of neurofilament proteins of low ( NF L ) , medium ( NF M ) , and high ( NF H ) molecular weight class . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The light ( NF L ) , mid sized ( NF M ) and heavy ( NF H ) neurofilament ( NF ) genes were probed with methylation sensitive restriction enzymes and patterns of methylation and expression of the NF genes were compared in tissues and cell lines of the mouse . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NFs ) , which are composed of NF L , NF M , and NF H , are required for the development of normal axonal caliber , a property that in turn is a critical determinant of axonal conduction velocity . ^^^ Only a combined increase of NF L with either NF M or NF H promotes radial axonal growth . ^^^ These results demonstrate that both NF M and NF H play complementary roles with NF L in determining normal axonal calibers . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Axonal neurofilaments are composed of light ( NF L ) , medium ( NF M ) , and heavy ( NF H ) subunits which are sequentially expressed during axonal development . ^^^ In retina , NF L and NF M appear prenatally , followed postnatally by NF H which occurs at about the time axons are reaching their target tissue . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Using antibodies that were directed at all three neurofilament ( NF ) epitopes , NF L , NF M , and NF H , we attempted to determine whether these neurons would have an altered cytoskeletal profile following the stress of transplantation , because previous studies have shown such changes following ischemia or direct brain injury . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We examined the effect of a 6 min depolarization with 60 mM KCl and 1 . 8 , 2 . 8 or 5 . 8 mM extracellular CaCl 2 on neurofilament proteins of high ( NF H ) , medium ( NF M ) and low ( NF L ) molecular weight in primary septohippocampal cultures . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments subunits ( NF H , NF M , NF L ) and glial fibrillary acidic protein ( GFAP ) were investigated in the hippocampus of rats after distinct periods of reperfusion ( 1 to 15 days ) following 20 min of transient global forebrain ischemia in the rat . ^^^ Three days after the insult , leucine incorporation significantly increased while the concentration NF H , NF M , and NF L were still diminished after 15 days of reperfusion . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilament ( NF ) proteins consist of three subunits of different molecular weights defined as NF H , NF M , and NF L . ^^^ Afferent mossy and climbing fibers in the medullary layer contained NF M and NF L already at birth , whereas NF H appeared later . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
When V 1 cells are transferred from the permissive temperature ( 33 degrees C ) to the restrictive temperature ( 39 degrees C ) , there is a decrease in expression of NF L and an increase in expression of NF H and glial fibrillary acidic protein in the flat V 1 cells . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Assembled as obligate heteropolymers requiring NF L and substoichiometric amounts of NF M and / or NF H , NF investment into axons is essential for establishment of axonal caliber , itself a key determinant of conduction velocity . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Antibodies against alpha internexin , the three neurofilament subunits ( NF L , NF M , NF H ) , vimentin , and glial fibrillary acidic protein ( GFAP ) were used to determine the relative expression of these proteins at different post natal stages of mouse retinal development . alpha Internexin is widely distributed in the process of amacrine cells , horizontal cells and retinal ganglion cells before post natal day 5 ( P 5 ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Cytoplasmic inclusion bodies that are accumulations of neurofilaments are the pathological hallmark of many neurodegenerative diseases and have been produced in transgenic mice by overexpression of mouse ( NF L and NF M ; light and medium chains , respectively ) and human ( NF M and NF H ; medium and heavy chains , respectively ) neurofilament subunits . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments , assembled from NF L ( 68 kd ) , NF M ( 95 kd ) , and NF H ( 115 kd ) , are the most abundant structural components in large myelinated axons , particularly those of motor neurons . ^^^ To examine whether mutation in neurofilament subunits causes or predisposes to ALS , we used single strand conformation polymorphism coupled with DNA sequencing to search for mutations in the entirety of the human NF L , NF M , and NF H genes from 100 familial ALS patients known not to carry mutations in superoxide dismutase 1 ( SOD 1 ) , as well as from 75 sporadic ALS patients . ^^^ Six polypeptide sequence variants were identified in rod and tail domains of NF L , NF M , or NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The studies described take advantage of the ability of endogenous NIF to incorporate purified biotinylated neurofilament triplet ( NFT ) proteins , NF L , NF M and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Finally , to precisely identify the nature of CR positive cells we accomplished double labelling immunofluorescence , using antisera against some nerve cell specific cytosquelettal proteins such as MAP 5 , MAP 2 , NF L , NF M , and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The monoclonal antibody NF 90 ( specific for the phosphorylated NF L , NF M and NF H subunits ) was used as neurofilament marker in fresh cryostat and Bouin fixed paraffin sections . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Treatment of RN46A cells with dbcAMP resulted in an increase in the steady state levels of both NF L and NF M , but not high M ( r ) ( 200 kDa , NF H ) neurofilament mRNA . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Assembly properties of amino and carboxyl terminally truncated neurofilament NF H proteins with NF L and NF M in the presence and absence of vimentin . ^^^ To understand the assembly characteristics of the high molecular weight neurofilament protein ( NF H ) , carboxyl and amino terminally deleted NF H proteins were examined by transiently cotransfecting mutant NF H constructs with the other neurofilament triplet proteins , low and middle molecular weight neurofilament protein ( NF L and NF M , respectively ) , in the presence or absence of cytoplasmic vimentin . ^^^ The results confirm that NF H can coassemble with vimentin and NF L but not with NF M into filamentous networks . ^^^ Deletions from the amino terminus show that the N terminal head is necessary for the coassembly of NF H with vimentin , NF L , or NF M / vimentin . ^^^ However , headless NF H or NF H from which the head and a part of the rod is removed can still incorporate into an NF L / vimentin network . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Mammalian neurofilaments are composed of three polypeptide subunits , NF L , NF M , and NF H , all of which are phosphorylated . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
These findings indicate that the assembly behaviors of NF H differ substantially from those of NF M and NF L , and that the interaction of NF H with NFs may be more dynamic than is generally recognized , especially during brain development and within specific compartments of mature neurons . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
To investigate the effect of short term depolarization on light ( NF L ) , middle ( NF M ) , and heavy ( NF H ) neurofilament proteins and determine the role played by calcium in that effect , mixed septo hippocampal cultures were exposed to 60 mM K+ for 6 min , in the presence of 0 to 11 . 8 mM Ca2+ . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In a mature mammalian neuron , NFs are co assembled from three subunits , NF L ( low ) , NF M ( medium ) , and NF H ( high ) , with molecular masses of 68 , 95 , and 115 kDa , respectively . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Variants of this sequence are also found in neurofilament low and high molecular mass proteins ( NF L and NF H ) and alpha internexin , and in mammalian NF L are known to be associated with in vivo phosphorylation sites . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In these Schwann cells the mRNAs for NF M and NF L ( the smallest polypeptide ) , but not NF H ( the largest neurofilament subunit ) , are coordinately expressed . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Western blot analysis demonstrated that P 19 EC cells contain significant levels of NF L protein in the insoluble fraction but undetectable levels of NF M and NF H protein in either the insoluble or total cell fractions . ^^^ Detectable levels of both NF L and NF M mRNA were present in P 19 EC cells whereas NF H mRNA remained below levels of detection , even by RT PCR analysis . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Steady state expression of transcripts for neurofilament ( NF ) proteins ( NF L , NF H ) and the high affinity NGF receptor , trkA was decreased by diabetes in the contralateral ganglia to the crush ( all P < 0 . 05 ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Wild type NF L or NF M , but not NF H , was able to complement and form a normal filament network with the tailless alpha internexin mutant , the alpha internexin head deletion mutant , or the alpha internexin mutant missing the entire tail and some amino terminal portion of the head domain . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The rates of transport of labeled high ( NF H ) , medium ( NF M ) , and low ( NF L ) molecular weight neurofilaments , and tubulin were faster in DFP treated birds than in controls after 3 days . ^^^ At 14 days , the peaks of NF H , NF M , and NF L in treated hens were distinctly behind the corresponding peaks in control hens . ^^^ This was again followed by an increase in transport of NF H and NF L , but not of NF M , so that the labeled NF H and NF L showed the same pattern in control and treated hens after 27 days . ^^^ The transient decrease in NF H and NF L axonal transport rate , and recovery correlated in a temporal manner with the previously reported increase of Ca2+ / calmodulin dependent protein kinase mediated phosphorylation of neurofilament proteins and inhibition of calpain activity in the sciatic nerve in OPIDN . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Computer assisted image analysis was used to study the immunoreactivity to NF L , NF M and NF H in human spiral ganglion cells . ^^^ The concentrations , represented by the relative mean grey of NF L , NF M and NF H , were calculated and compared . ^^^ The cell area mean grey correlations for NF L , NF M and NF H were analysed and calculated , showing that NF M is more specific to the larger cells ( type 1 ? ) and NF H is more specific for the smaller cells ( type 2 ? ) , while NF L is non specific for cell size . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Previous studies have shown that rodent neurofilaments ( NF ) are obligate heteropolymers requiring NF L plus either NF M or NF H for filament formation . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Antagonistic roles of neurofilament subunits NF H and NF M against NF L in shaping dendritic arborization in spinal motor neurons . ^^^ Neurofilaments ( NFs ) , a major component of the neuronal cytoskeleton , are composed of three polypeptide subunits , NF H , NF M , and NF L , and are abundant in large dendritic trees . ^^^ By overexpressing each of the three NF subunits in transgenic mice , we altered subunit composition and found that increasing NF H and / or NF M inhibited dendritic arborization , whereas increasing NF L alleviated this inhibition . ^^^ However , these changes were reversed by elevating the level of NF L with either NF H or NF M . ^^^ Thus , NF L antagonizes NF H and NF M in organizing the NF network and maintaining a lower ratio of NF H and NF M to NF L is critical for the growth of complex dendritic trees in motor neurons . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Here we show that mice with a null mutation in the mid sized NF ( NF M ) subunit have dramatically decreased levels of light NF ( NF L ) and increased levels of heavy NF ( NF H ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Thus , the NF L promoter differs from the mouse NF heavy ( NF H ) and mid sized ( NF M ) promoters in that no specific sequence within the immediate upstream NF L promoter region ( 154 to 29 ) appears to be responsible for enhancement or brain specific transcription . ^^^ We show that the order of strength of the three NF promoters is NF H > NF M > NF L and identify sequences that can increase or reduce transcription when placed in front of heterologous NF promoters . ^^^ We conclude that the NF L promoter is a modular , weak and promiscuous promoter whose regulation differs from NF H or NF M . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
To detect NF polypeptide subunits , commercially available monoclonal antibodies against mammalian NF L ( 68 kDa ) , NF M ( 160 kDa ) , phosphorylated ( P ) and non phosphorylated ( nP ) NF H ( 200 kDa ) epitopes were used . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In NF L deleted animals , levels of the two remaining neurofilament subunits , NF M and NF H , are markedly reduced in axons but are elevated in motor neuron cell bodies . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The two transgenic lines show no elevation of NF L , neurofilament middle chain ( NF M ) , or neurofilament heavy chain ( NF H ) , and transgenic NF L ( Asp ) represents only a minor proportion of total NF L protein . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Taxol suppressed polymerization between neurofilament light and heavy ( NF L and NF H ) , and MB 2 cell extract rescued it . ^^^ We , therefore , suggest the acceleration of polymerization between NF L and NF H as the new possible role of Bcl 2 . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments are obligate heteropolymers requiring the NF L subunit together with either the NF M or the NF H subunit for polymer formation . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In this paper we present AFM images of native neurofilaments isolated from bovine spinal cord , composed of NF L , NF M and NF H , and filaments polymerized in vitro from purified NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Remarkably , the motor neuronopathy in transgenic mice overexpressing human NF heavy ( NF H ) subunits was rescued by the co expression of a human NF L transgene at levels that restored a correct stoichiometry of NF L to NF H subunits . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Antibodies against alpha internexin , neurofilament triplet proteins ( NFTPs ; NF L , NF M , and NF H ) and peripherin were used to determine their expression at different developmental stages . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
METHODS : In this study we used an ELISA technique to measure IgG antibody levels against three neurofilament polypeptides ( NF L , 70 kD ; NF M , 150 kD ; and NF H , 200 kD ) in RA patients with peripheral neuropathy , and compared them with the antibody levels in uncomplicated RA controls and healthy volunteers . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Using RT PCR , western blot and enzyme and fluorescence immunocytochemical techniques , the three isoforms of neurofilament proteins ( NFPs ) , namely NF L ( NFP 68 kDa ) , NF M ( NFP 160 kDa ) and NF H ( NFP 200 kDa ) were found in Sertoli and Leydig cells of human testes . ^^^ In protein preparations from the same testicular components , western blot analysis detected bands with molecular weights characteristic for NF H , NF M and NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Immunocytochemistry was performed to evaluate the expression of five of the most represented neurofilaments proteins ( the low , NF L , medium , NF M , and heavy , NF H , molecular weight subunits , alpha internexin and peripherin ) and of two of the microtubule associated proteins ( MAPI and MAP2a+2b ) . ^^^ NF L and NF H expression appeared later , by day E16 . 5 , and was weak for the entire pre and postnatal life . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In order to see whether NF antibodies can be used as a specific marker for ganglion cells , antibodies raised against the low ( NF L ) , middle ( NF M ) and high ( NF H ) molecular weight subunits of NF were employed to stain retinal whole mounts of adult hamsters after pre labelling the ganglion cells with Granular Blue . ^^^ It was found that NF L and NF H antibodies labelled 38 , 777 and 17 , 750 cells in the ganglion cell layer respectively . ^^^ By co localization with GB labelled cells , 88 % of NF L positive cells and 91 % of NF H positive cells were found to be ganglion cells . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
At 3 hours ( h ) , 6 h , 24 h , 3 days , 7 days , and 10 days post trauma , immunohistochemical analysis was performed to determine axonal accumulation of NF light ( NF L ) , the rod and sidearm domains and sidearm phosphorylation states of NF medium ( NF M ) , and heavy ( NF H ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NFs ) are neuron specific intermediate filaments ( IFs ) composed of three different subunits , NF L , NF M , and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Mammalian neurofilaments are assembled from the light ( NF L ) , midsized ( NF M ) , and heavy ( NF H ) neurofilament proteins . ^^^ While NF M and NF H can not self assemble into homopolymers , the data concerning NF L has been more contradictory . ^^^ To determine if mouse NF L can homopolymerize in mouse axons , NF M and NF H null mutants were bred to create a line of double mutant animals . ^^^ Thus in vivo rodent neurofilaments are obligate heteropolymers requiring NF L plus either NF M or NF H to form a filamentous network . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
They are formed by the co assembly of three subunits : NF L , NF M , and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
I . p . administration of TH to 2 day old hypothyroid rats resulted in a 2 fold increase in cerebral NF M and NF L mRNAs , while administration to 15 day old hypothyroid rats led to a 1 . 5 to 3 fold increase in NF H mRNA within 2 4 h of hormone injection . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In NF M / mice , the velocity of axonal transport of NF light ( NF L ) and NF H proteins was increased by about two fold , whereas the steady state levels of assembled NF L were reduced . ^^^ Immunohistochemistry of spinal cord from the NF M / ; NF H / mice revealed enhanced NF L staining in the perikaryon of motor neurons but a weak NF L staining in axons . ^^^ In addition , axonal transport studies carried out by the injection of [ 35S ] methionine into spinal cord revealed after 30 days very low levels of newly synthesized NF L proteins in the sciatic nerve of NF M / ; NF H / mice . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Extra neurofilament NF L subunits rescue motor neuron disease caused by overexpression of the human NF H gene in mice . ^^^ The finding that extra NF L can protect against NF H mediated pathogenesis is of potential importance for ALS , particularly for cases with NF H abnormalities . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
No apparent quantitative changes of NF L and NF H were found between the Alzheimer and control brains , and there were also no significant differences in the mean molar ratio of NF L to NF H between them . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments comprise three subunit proteins ; neurofilament light , middle and heavy chains ( NF L , NF M and NF H ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Overexpression of neurofilament subunit NF L and NF H extends survival of a mouse model for amyotrophic lateral sclerosis . ^^^ G93A mice overexpressing either NF L or NF H developed ALS later and survived longer than the G93A mice on a wild type background . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments are composed of three polypeptide subunits ( NF H , NF M and NF L ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NFs ; made by copolymerization of three intermediate filament proteins NF L , NF M , and NF H , for light , medium , and heavy ) constitute the most abundant cytoskeletal structure in large myelinated axons . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Gel shift studies were used to compare RNA binding complexes that assemble on the 3 ' UTR of the heavy ( NF H ) , mid sized ( NF M ) and light ( NF L ) NF mRNAs when radioactive RNA probes are incubated with high speed supernatants ( S 100 ) of rat brain homogenates . ^^^ Similar RNA binding complexes formed on probes to the 3 ' UTRs of NF L and NF H mRNAs . ^^^ Their binding sites were localized to a 36 nt sequence in the mid distal region of the NF H 3 ' UTR and to a 45 nt sequence at the proximal edge of the 3 ' UTR of the NF L transcript . ^^^ The 43 kDa protein also bound directly to NF L and NF H probes in Northwestern blots . ^^^ Functional studies showed that deletion of the binding sites markedly increased expression of a luciferase reporter gene containing the 3 ' UTR of NF L or NF H by stabilizing the fusion transcripts . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The neurofilament ( NF ) proteins ( NF H , NF M , and NF L for high , medium , and low molecular weights ) play a crucial role in the organization of neuronal shape and function . ^^^ Decreased levels of nonphosphorylated NF H ( 42 32 % ) , NF M ( 14 9 % ) and NF L ( 30 29 % ) were found in the prefrontal cortex of opioid addicts compared with sex , age , and postmortem delay matched controls . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Northern blot analysis showed that in TH deficient ( THdef ) neurons , the expression of all three NF mRNAs ( NF L , NF M and NF H ) are retarded by 50 70 % at days 7 , 15 and 25 of culture compared with their TH supplemented ( THsup ) counterparts . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Western blot analysis showed increased expression of middle molecular weight neurofilament protein ( NF M ) , and decreased expression of high molecular weight ( NF H ) and low molecular weight ( NF L ) neurofilament proteins in the 2 M urea extracts of spinal cord particulate fraction . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
RT PCR demonstrated the presence of NF L , NF M and NF H mRNAs in intact sciatic nerve , as well as in proximal and distal stumps of severed nerves . ^^^ Northern blot analysis of severed nerve detected NF L and NF M , but not NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Cytoskeletal abnormalities have been reported in cases of amyotrophic lateral sclerosis ( ALS ) including abnormal inclusions containing neurofilaments ( NFs ) and / or peripherin , reduced mRNA levels for the NF light ( NF L ) protein and mutations in the NF heavy ( NF H ) gene . ^^^ Nonetheless , mice with null mutations for NF L or for both NF M and NF H genes developed severe atrophy of ventral and dorsal root axons . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In NF immuocytochemical staining , there were strong responses to NF H and NF M in all cultures , but not to NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilament proteins ( NFs ) are made by co polymerization of three intermediate filament proteins , NF L , NF M and NF H and constitute the most abundant cytoskeletal element in large myelinated axons . ^^^ The aim of our study was to examine functional properties of large myelinated axons with altered morphology from mice bearing a targeted disruption of each NF genes ( NF L / , NF M / and NF H / mice ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Axonal maturation in situ is accompanied by the transition of neurofilaments ( NFs ) comprised of only NF M and NF L to those also containing NF H . ^^^ Proximal biotinylated NF H accumulation paralleled that of endogenous NF immunoreactivity ; however , distal most biotinylated NF H accumulation dramatically exceeded that of endogenous NFs and microinjected NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Previous studies in this laboratory have shown an increased level of medium molecular weight NF ( NF M ) and decreased levels of high and low molecular weight NF ( NF H , NF L ) proteins in the spinal cord of DFP treated hens . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilament proteins NF L , NF M and NF H in brain of patients with Down syndrome and Alzheimer ' s disease . ^^^ We evaluated immunoreactive NF H , NF M and NF L levels using Western blotting in brain regions temporal , occipital cortex and thalamus of patients with DS ( n = 9 ) , AD ( n = 9 ) and controls ( n = 12 ) . ^^^ We found significantly increased NF H in temporal cortex ( controls : means 0 . 74+ / 0 . 39 SD ; DS : means 3 . 01+ / 2 . 18 SD ) of DS patients and a significant decrease of NF L in occipital cortex of DS and AD patients ( controls : means 1 . 19+ / 0 . 86 SD ; DS : means 0 . 35+ / 0 . 20 ; AD : 0 . 20+ / 0 . 11 SD ) . ^^^ The increase of NF H in DS or the decrease of NF L in DS and AD leaves the other NFs unchanged , which points to dysregulation in DS and AD and raises the question of impaired structural assembly of neurofilaments . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
NF H and its mRNA were drastically enhanced in expression in these mice , whereas enhancement in expression of NF L and its mRNA was slight . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The amount of NF H and NF L remaining after proteolysis was determined by SDS PAGE and quantitative immunoblotting . ^^^ Proteolysis of the NF H and NF L polypeptides was not affected by 2 , 5 HD exposure . ^^^ Degradation of the HMW material containing NF H or NF L was retarded when comparing with degradation of the NF H and NF L polypeptides , respectively , from control samples , but not as compared to the corresponding NF polypeptides from 2 , 5 HD treated samples . ^^^ No differences were found between the proteolytic rates of NF L and NF H from exposed cells as compared with those subunits from control cells . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The increase in the levels of NF H and NF M was 1 . 7 and 1 . 5 fold ( P < 0 . 01 ) as determined by monoclonal antibody SMI 33 , and was 1 . 6 fold ( P < 0 . 01 ) in NF L using antibody NR 4 . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In the present study , we have used immunoblotting and immunocytochemical methods to study the light ( NF L ) , medium ( NF M ) , and heavy ( NF H ) molecular weight NF proteins in cultured organotypic hippocampal slices during the in vitro maturation and the changes after kainic acid ( KA ) treatment . ^^^ A few weakly NF L positive granule cells with fibers were detected in each age group , whereas NF M and NF H positive granule cells first appeared in the older cultures . ^^^ After the treatment , the immunoblot signal of NF L and NF M markedly decreased , whereas that of NF H almost completely disappeared . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The neurofilament triplet proteins : NF L , NF M and NF H were purified by DE 52 anion exchange chromatography in the presence of 6 mol / L urea . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The class 4 neuronal intermediate filament ( IF ) family proteins includes the neurofilament ( NF ) triplet proteins NF L , NF M , and NF H and also the more recently characterized alpha internexin NF 66 . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments , composed of three polypeptide subunits , NF L , NF M , and NF H , are major cytoskeletal elements in large neurons with long axons . ^^^ We also reveal that a decrease in NF L leads to an increase in NF M and NF H subunits in cell bodies and their reduction in dendrites . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilament ( NF ) , a major neuronal intermediate filament , is composed of three subunits , NF L , NF M , and NF H . ^^^ Although the E segments of NF L and NF M are phosphorylated by casein kinases , it has not been observed in NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
These alpha internexin positive cells coimmunostained for the neuronal markers MAP 2 , type 3 beta tubulin , and tau , and also bound tetanus toxin , but were negative for the oligodendrocyte marker GalC and also for the neurofilament triplet proteins NF L , NF M , and NF H , markers of more mature neurons . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilament ( NF ) subunits NF H , NF M and NF L are hyperphosphorylated and elevated in Alzheimer disease ( AD ) brain . ^^^ We found : ( 1 ) , that the levels of phosphorylated NF H / M ( pNF H / M ) , non phosphorylated NF H / M ( npNF H / M ) and NF L were significantly higher ( pNF H / M , approximately 12 24 fold ; npNF H / M , approximately 3 4 fold ) in neurologically healthy aged people than young control individuals ; ( 2 ) , that in AD , the levels of npNF H / M , and NF L were similar to vascular dementia ( VaD ) , and higher than in age matched controls ; and ( 3 ) , that the levels of pNF H / M were significantly higher than in aged controls , non AD neurological disorders and VaD . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
A combination of immunostaining , immunoblot , and microarray analysis showed that 293 cells express the neurofilament ( NF ) subunits NF L , NF M , NF H , and a internexin as well as many other proteins typically found in neurons . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
This study investigated whether progression in MS is associated with CSF antibodies to the 68 kd light neurofilament subunit ( NF L ) , an axonal cytoskeletal protein , and compared this with antibodies against tubulin and the heavy neurofilament subunit ( NF H ) . ^^^ METHODS : IgG to NF L , tubulin , and NF H was measured by immunoassay in matched CSF and serum samples from patients with relapsing remitting MS ( RRMS ; n = 39 ) , primary progressive MS ( PPMS ; n = 10 ) , and secondary progressive MS ( SPMS ; n = 18 ) ; patients with other inflammatory ( n = 21 ) and noninflammatory ( n = 40 ) neurologic diseases ; and healthy controls ( n = 12 ) . ^^^ Anti NF L , antitubulin , and anti NF H levels correlated with the duration of disease before lumbar puncture and Expanded Disability Status Scale levels . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In young mutant animals , fast axonal transport appeared to be intact , but NF H , as well as NF M and NF L , accumulated in the cell bodies of peripheral sensory neurons accompanied by a reduction in sensory axon caliber . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The high salt Triton insoluble cytoskeletal fraction is enriched in neurofilament subunits ( NF H , NF M and NF L ) , vimentin and glial fibrillary acidic protein ( GFAP ) , while the low salt Triton insoluble cytoskeletal fraction contains detergent insoluble cytoskeletal elements such as intermediate filament subunits and tubulins . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We used immunocytochemistry to examine changes in the expression of heavy , medium and light neurofilament ( NF H , NF M , NF L ) proteins in the afferent terminals of vestibular sensory epithelia after IDPN exposure in rats . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Compared with age matched controls , neurofilament heavy ( NF H ) ( 3 . 3 fold ) and neurofilament medium ( NF M ) ( 2 . 5 fold ) , but not neurofilament light ( NF L ) , subunits accumulated in the proximal axon of sensory neurons of the lumbar dorsal root ganglia ( DRG ) in untreated diabetic rats . ^^^ The sural nerve of untreated diabetic rats showed a 50 % decrease in the levels of NF H and NF M , but not NF L , subunits ; NT 3 only partially normalized the defect in NF M expression . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Lysine rich neurofilament protein subunits NF H and NF M are more susceptible than lysine poor NF L and beta tubulin to 1 , 2 DAB . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
A 1 hybrid neurons express neuron specific markers such as neurofilament L ( NF L ) , NF M , NF H , MAP 2 , and beta tubulin 3 . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
To further investigate the toxic properties of IF protein inclusions , we generated NF L null mice that co express both peripherin and NF H transgenes . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilaments ( NF ) are neuronal intermediate filaments formed by three different subunits : high ( NF H ) , medium ( NF M ) and light ( NF L ) . ^^^ LM for NF of control nerves showed a slightly different pattern and intensity for each subunit , with more intense staining of NF M and NF H and less intense staining of NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neurofilament assembly requires at minimum the polymerization of neurofilament light chain ( NF L ) with either neurofilament medium chain ( NF M ) or neurofilament heavy chain ( NF H ) subunits , but requirements for their axonal transport have long been controversial . ^^^ They also show that NF M can partner with intermediate filament proteins other than the NF H and NF L subunits in neurons to support slow transport and possibly other functions of neuronal intermediate filaments . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Immunoprecipitation from pulse chase radiolabeled cells demonstrated transient co precipitation of newly synthesized NF H with Vm , followed by increasing co precipitation with NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Five major types of intermediate filament ( IF ) proteins are expressed in mature neurons : the three neurofilament proteins ( NF L , NF M , and NF H ) , alpha internexin , and peripherin . ^^^ Substantial developmental loss of motor axons was detected in mice lacking NF L and in double knockout NF M ; NF H mice , supporting the view of a role for IFs in axon stabilization . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In the present study , we have used Western blotting and immunocytochemistry to study the low ( NF L ) , medium ( NF M ) , and high ( NF H ) molecular weight NF proteins ; phosphorylated epitopes of NF M and NF H ; and a nonphosphorylated epitope of NF H in the early postnatal ( through P 1 P21 ) development of the rat hippocampus . ^^^ During the first postnatal week , NF M was the most abundantly expressed NF , followed by NF L , whereas the expression of NF H was very low . ^^^ Through P 7 P14 , the expression of NF H increased dramatically and later began to plateau , as also occurred in the expression of NF M and NF L . ^^^ At P 7 , CA 3 pyramidal neurons were strongly immunopositive for NF L and NF H , but not for NF M . ^^^ The axons of granule cells , the mossy fibers ( MFs ) , were NF L and NF M positive through P 7 P21 but were NF H immunonegative at all ages . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Experimentally motor neuron disease can be induced in transgenic mice by overexpressing the mouse neurofilament light subunit ( NF L ) , the human heavy subunit ( NF H ) , or mouse peripherin . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
They are classed into three groups according to their molecular masses : neurofilament heavy , middle and light chains ( NF H , NF M and NF L ) . ^^^ NF H and NF M are distinct from NF L as they contain a carboxyl terminal tail domain , which appears to form connections with adjacent structures and other neurofilaments . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neither skein like inclusions nor LBLIs expressed alpha B crystallin , neurofilament protein ( NF L , NF M and NF H subunits ) , alpha internexin , actin or alpha synuclein . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Since the RGC cytoskeleton exhibits differential damage in diseases such as glaucoma , we examined the distribution of light , medium , and heavy NF subunits ( NF L , NF M , and NF H respectively ) within normal human and porcine retinas , as a function of RGC soma size and eccentricity . ^^^ RESULTS : NF H and NF M were distributed widely within all RGC somata and dendrites , whereas NF L was more restricted to the perinuclear area . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Cytoskeleton proteins beta actin ( betaA ) , alpha actinin ( Act ) , tubulin beta 3 ( betaIII ) , microtubule associated protein 1 ( MAP 1 ) , neurofilaments NF L , NF M and NF H and neuron specific enolase ( NSE ) , a marker for neuronal density , were determined by immunoblotting . ^^^ In DS brain betaIII , NF H and NF M , in AD brain NF M and NF H and in PD brain NF L , NF M and NF H were significantly reduced . ^^^ Stoichiometry of cytoskeleton proteins in control brain revealed the following relations : betaA : Act : betaIII : MAP 1 : NF L : NF M : NF H = 1 . 0 : 0 . 8 : 3 . 8 : 2 . 4 : 3 . 2 : 2 . 2 . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Cytoskeletal proteins ( NF L , NF M , NF H and beta tubulin ) in each fraction were determined by immunoblot analysis . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
To deeply investigate the alterations of cytoskeletal proteins in HD peripheral neuropathy , the relative levels of NF L , NF M , NF H , alpha tubulin , beta tubulin and beta actin of rat sciatic tibial nerves were determined by SDS PAGE and immunoblotting . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
They are composed of three different polypeptides : light ( NF L ) , medium ( NF M ) and heavy ( NF H ) subunits . ^^^ In control cases , the immunoreaction in the cytoplasm of neurons was weak for phosphorylated NF H , strong for NF M and weak for NF L . ^^^ Furthermore , we also observed dilated axons ( spheroids ) , which were immunopositive for NF H but negative for NF M and NF L . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In this work the timing of expression of nestin , peripherin , internexin , and the neuronal intermediate filament triplet [ polypeptide subunits of low ( NF L ) , medium ( NF M ) , and high ( NF H ) molecular weight ] was investigated in the developing fetal and postnatal mouse vomeronasal organ ( VNO ) by means of immunohistochemistry . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Three IF types were studied in vitro : recombinant murine desmin , recombinant human keratin K5 / K14 and neurofilaments isolated from rat brains , which are composed of the neurofilament triplet proteins NF L , NF M and NF H . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Then , the contents of six cytoskeletal protein ( NF L , NF M , NF H , alpha tubulin , beta tubulin , and beta actin ) in both fractions were determined by immunoblotting . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Abnormal neuronal aggregates of alpha internexin and the three neurofilament ( NF ) subunits , NF L , NF M , and NF H have recently been identified as the pathological hallmarks of neuronal intermediate filament ( IF ) inclusion disease ( NIFID ) , a novel neurological disease of early onset with a variable clinical phenotype including frontotemporal dementia , pyramidal and extrapyramidal signs . alpha Internexin , a class 4 IF protein , a major component of inclusions in NIFID , has not previously been identified as a component of the pathological protein aggregates of any other neurodegenerative disease . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We used immunohistochemistry for light microscopy with monoclonal antibodies against three different NF subunits , high ( NF H ) , medium ( NF M ) , and light ( NF L ) . ^^^ The NN 18 clone monoclonal antibody recognized a protein of approximately 160 kDa , similar to the mammalian NF M protein , but NF L and NF H were not recognized . ^^^ Our results agree with previous studies on crustacean NF proteins that have reported negative immunoreactions against NF H and NF L subunits and positive immunoreactions against the mammalian NF M subunit . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In Western blotting , the expression of the phosphorylation independent epitopes of NF L , NF M , and NF H rapidly but transiently increased after the treatment , whereas the phosphorylated NF M remained elevated for 7 days . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
These data , confirmed by real time RT PCR , identified differential expression of intermediate neurofilament transcripts ( i . e . , Nefh , Nef 3 , and Nfl ) among the subdivisions of the cochlear nucleus . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Immunoblotting analysis of olfactory epithelium extracts demonstrated the presence of only the 200 kDa ( NFH ) polypeptide ; the 68 kDa ( NFL ) and 160 kDa ( NFM ) neurofilaments were not detected . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
These subunits are referred to as the low ( NFL ) , middle ( NFM ) , and high ( NFH ) Mr subunits , respectively . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The nfh gene shares two common intron positions with the nfl and nfm genes , but has an additional intron that occurs at a location equivalent to one of the introns in non neuronal intermediate filament coding genes . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Overexpression of the human NFM subunit in transgenic mice modifies the level of endogenous NFL and the phosphorylation state of NFH subunits . ^^^ Neurofilaments ( NFs ) , the major intermediate filaments of central nervous system ( CNS ) and peripheral nervous system ( PNS ) neurons , are heteropolymers formed from the high ( NFH ) , middle ( NFM ) , and low ( NFL ) molecular weight NF subunits . ^^^ The increased levels of mNFL appear specific to NFM because previous studies of transgenic mice overexpressing either NFL or NFH did not result in increased expression of either of the other two NF subunits . ^^^ Taken together , these studies imply that NFM may play a dominant role in the in vivo regulation of the levels of NFL protein , the stoichiometry of NF subunits , and the phosphorylation state of NFH . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Transcriptional changes of NFs including high molecular weight neurofilament ( NF H ) , middle molecular weight neurofilament ( NF M ) , low molecular weight neurofilament ( NF L ) , and glyceraldehydes 3 phoaphate dehydrogenase ( GAPDH ) as inner inference in cerebral cortexes and spinal cords were analyzed by semi quantitative reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ However , in spinal cords , there was only NF M decreased , both of NF H and NF L kept unaffected . ^^^ NF H and NF M proteins in supernatant of cerebral cortexes exhibited significant increase , while NF L level showed remarkable decline . ^^^ In spinal cords , apart from NF L in pellet were significantly increased , both of NF H and NF M in pellet and supernatant , as well as NF L in supernatant fractions were manifested dramatic reduction compared with the pattern of control . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The developmentally regulated intracellular intermediate filament protein neurofilament ( NF ) , composed of the light ( NF L ) , medium ( NF M ) and high ( NF H ) molecular weight isoforms , is expressed abundantly in nerve cells but its significance in nerve cell survival in stress situations in the brain is unknown . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Compared with the control , the levels of NF L , NF M , NF H , beta actin , tau , MAP 2 proteins decreased and the level of alpha tubulin increased in high ACR group , the levels of alpha tubulin , beta tubulin and MAP 2 increased in low ACR group . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Microtubule associated protein 1B ( MAP1B ) and the intermediate filament ( IF ) protein nestin are enriched in neural progenitors , whereas MAP2B , phosphorylated and non phosphorylated forms of medium ( NFM ) and high ( NFH ) molecular weight neurofilament ( NF ) proteins , as well as the light NF subunit ( NFL ) and IF protein alpha internexin are expressed in developing and mature neurons . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Formation of protein dimers involving alpha internexin , peripherin , and the neurofilament ( NF ) proteins NFH , NFM , and NFL was investigated by partial renaturation of various combinations of individually purified subunits in buffered 2 M urea . ^^^ Combining this method with Western blot analysis , disulfide cross linking , and SDS polyacrylamide gel electrophoresis in the second dimension showed that NFL readily forms significant amounts of heterodimer with NFH , NFM , alpha internexin , or peripherin in the presence of 2 M urea . alpha Internexin and peripherin also formed heterodimers with NFH or NFM under these conditions . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
The general innervation pattern of taste papillae and taste bud primordia was revealed immunohistochemically using antibodies against protein gene product 9 . 5 ( PGP9 . 5 ) , neurofilament H ( NFH ) , neurofilament L ( NFL ) , neurone specific enolase ( NSE ) , and tubulin . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Median NFL , alpha internexin , and peripherin steady state mRNA levels were significantly reduced in the lateral motor neuron cell column ( p < 0 . 05 ) of ALS cases , while neither NFM nor NFH mRNA levels were altered . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Dose dependent selective suppression of light ( NFL ) and medium ( NFM ) but not heavy ( NFH ) molecular weight neurofilament mRNA levels in acute aluminum neurotoxicity . ^^^ Northern blot analysis was performed with cDNA probes for light ( NFL ) , medium ( NFM ) , and heavy ( NFH ) neurofilament subunit protein or with oligonucleotide probes for alpha tubulin or actin . ^^^ These experiments indicate that intracisternally administered AlCl 3 acutely suppresses NFL and NFM mRNA levels without affecting those of NFH . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We have previously demonstrated an acute , dose dependent suppression of low molecular weight neurofilaments ( NFL ) and intermediate molecular weight neurofilaments ( NFM ) steady state mRNA levels while sparing those of high molecular weight ( NFH ) mRNA 48 hours ( h ) following the intracisternal inoculation of AlCl 3 in young adult New Zealand white rabbits . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
A significant increase in peripherin levels was seen at days 1 2 , in the midsized ( NFM ) and low molecular weight ( NFL ) NF subunits at days 2 3 , and in the high molecular weight ( NFH ) NF subunit at days 5 6 . ^^^ Co immunoprecipitation of NFH with NFL , NFM with NFL , NFM with alpha internexin , and alpha internexin with peripherin demonstrated that the intermediate filament cytoskeleton in cultured sensory neurons is a highly integrated structure . . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
In these later animals , immunolabelling for NFL and NFM ( NFH subunit excepted ) was increased by 142 and 63 % , respectively , indicating reduction of axonal abnormalities . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
NF filaments are composed of three subunit proteins , NFL , NFM , and NFH , and are major constituents of the axonal cylinder . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
METHODS : In 51 patients with MS ( 21 relapsing remitting , 20 secondary progressive , 10 primary progressive ) , levels of heavy and light neurofilaments ( NfH and NfL ) and antibodies to neurofilaments ( anti NfL and NfH ) as well as the total immunoglobulin G ( IgG ) were analyzed . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Matrix assisted laser desorption / ionization ( MALDI ) time of flight ( TOF ) mass spectrometry ( MS ) shows molecular masses for bovine NFP subunits of 63 , 105 , and 125 kDa for NFL , NFM , and NFH . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
To elucidate the role of NF subunit proteins in tau aggregate formation and to test the hypothesis that NFs are pathological chaperones in the formation of intraneuronal tau inclusions , we crossbred previously described tau ( T 44 ) Tg mice overexpressing the smallest human tau isoform with knock out mice devoid of NFL ( NFL / ) or NFH ( NFH / ) . ^^^ Depletion of NF subunit proteins from the T 44 mice ( i . e . , T 44 ; NFL / and T 44 ; NFH / ) , in particular NFL , resulted in a dramatic decrease in the total number of tau positive spheroids in spinal cord and brainstem . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Radiolabeled aRNA from single neurons was used to probe cDNA arrays containing the low ( NFL ) , medium ( NFM ) and high ( NFH ) molecular weight neurofilament isoform , alpha internexin , desmin , vimentin , peripherin ( PRPH ) , nestin , and glial fibrillary acidic protein ( GFAP ) cDNAs . ^^^ Increased expression of nestin , alpha internexin , PRPH , vimentin , NFL , NFM , and NFH mRNAs was found in DN when compared with the three control neuronal subtypes . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We have examined the steady state levels of intermediate filament mRNA in amyotrophic lateral sclerosis using the RNAse protection assay ( NFL , NFM , NFH ; corrected against GAPDH ) or by PCR ( peripherin , alpha internexin , nestin , and vimentin ; corrected against beta actin ) . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
We investigated the immunohistochemical stainability of phosphorylated tau and the light ( NFL ) , intermediate ( NFM ) , and heavy ( NFH ) neurofilament proteins in postmortem brain tissue from 8 patients with frontotemporal dementia ( FTD ) , for comparison with 6 patients with Alzheimer ' s disease ( AD ) , and 6 normal controls . ^^^ The antibodies against NFM and NFH stained positive in FLD , AD , and controls , whereas the staining for NFL was negative in all FLD cases and positive in 1 AD case and in the controls . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Microtubule associated protein 2 ( MAP 2 ) and light ( NFL ; 68 kDa ) , medium ( NFM ; 160 kDa ) and heavy ( NFH ; 200 kDa ) neurofilament subunit immunostainings were analyzed employing morphometric parameters . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Neuronal intermediate filaments consist of the NFL subunit linked with NFM and NFH , and their alterations have been proposed as a pathogenesic cause in motor neuron diseases . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
Abnormal neuronal aggregates of alpha internexin and the three neurofilament ( NF ) subunits , NFL , NFM , and NFH have recently been identified as the signature lesions of neuronal intermediate filament ( IF ) inclusion disease ( NIFID ) , a novel neurological disease of early onset with a variable clinical phenotype including frontotemporal dementia , pyramidal and extrapyramidal signs . ^^^
Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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Interacting proteins: P12036 and P07196	Pubmed	SVM Score :0.0
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