| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.59858374 |
| Plasminogen binds specifically to alpha enolase on rat neuronal plasma membrane . 0.59858374^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.50591706 |
| Here we show that Plg activates the mitogen activated protein kinases MEK and ERK which leads to alpha enolase ( alpha ENO ) gene expression not only in fibroblasts , but also in peripheral blood mononuclear cells . 0.50591706^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Role of cell surface lysines in plasminogen binding to cells : identification of alpha enolase as a candidate plasminogen receptor . ^^^ A major protein of Mr 54 , 000 was recovered and identified as alpha enolase by immunochemical and functional criteria . alpha Enolase was present on the cell surface and was capable of binding plasminogen in ligand blotting analyses . ^^^ Plasminogen binding activity of a molecular weight similar to alpha enolase also was present in a variety of other cell types . ^^^ Carboxypeptidase B treatment of alpha enolase abolished its ability to bind plasminogen , consistent with the presence of a C terminal lysyl residue . ^^^ Thus , cell surface proteins with carboxy terminal lysyl residues appear to function as plasminogen binding sites , and alpha enolase has been identified as a prominent representative of this class of receptors . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Moreover , immunopurified alpha enolase enhanced plasminogen activation by tissue plasminogen activator and blocked the binding of plasminogen to alpha 2 antiplasmin , mimicking functions arising from the association of plasminogen with cells . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Comparing Pli R with alpha enolase ( a candidate for plasminogen receptor in U 937 cells ) we have found a high homology between both proteins , but not an identity . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The 45 kDa protein could be distinguished from alpha enolase which also binds plasminogen by : ( 1 ) significant differences in the profile of retention times of CNBr degradation fragments on reversed phase HPLC ; and ( 2 ) partial peptide sequencing of one of the CNBr degradation fragments of the 45 kDa protein . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Furthermore , molecules that have been implicated as candidate plasminogen receptors , gangliosides , and an alpha enolase related molecule , also interacted with t PA . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The human ENO 1 gene product ( recombinant human alpha enolase ) displays characteristics required for a plasminogen binding protein . ^^^ Previously , a 54 kDa plasminogen receptor found on the surface of U 937 cells was identified as an alpha enolase like molecule . ^^^ The aims of this study were to determine whether recombinant alpha enolase ( r alpha enolase ) , encoded by ENO 1 , was a plasminogen binding protein and to generate polyclonal antibodies against this antigen . ^^^ Plasminogen specifically bound r alpha enolase with a Kd 1 . 9 microM and approached saturation at 10 microM . ^^^ Lysine dependent plasminogen binding to r alpha enolase was demonstrated by a greater than 80 % inhibition of binding by the lysine analogues epsilon amino caproic acid and tranexamic acid , whilst only 14 % inhibition occurred with the arginine analogue benzamidine . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Identification of an epitope of alpha enolase ( a candidate plasminogen receptor ) by phage display . ^^^ An alpha enolase related molecule ( alpha ERM ) is present on the surfaces of neutrophils , monocytes and monocytoid cells and has the capacity to specifically bind plasminogen , suggesting that alpha ERM may function as a plasminogen receptor . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Increased expression of alpha enolase in c jun transformed rat fibroblasts without increased activation of plasminogen . ^^^ However , no difference was observed between normal and transformed cells in formation of plasmin , suggesting that upregulation of alpha enolase may contribute to an increased metabolic capacity , but not to increased plasminogen activation . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Densitometry of autoradiograms of the 125I plasminogen ligand blots of U 937 cell membranes revealed that membrane associated alpha enolase , actin and annexin 2 showed minimal changes in 125I plasminogen binding following CpB treatment of intact cells , suggesting that these proteins are not accessible to CpB on the U 937 cell surface and most likely do not serve as profibrinolytic plasminogen receptors on U 937 cells . ^^^ In contrast , densitometry of autoradiograms of 125I plasminogen ligand blots of monocyte membranes revealed that 125I plasminogen binding to alpha enolase was reduced 71 % by treatment of intact cells with CpB , while binding to annexin 2 was reduced 14 % . ^^^ Thus , a portion of membrane associated alpha enolase and annexin 2 expose carboxyl terminal lysines that are accessible to CpB on the peripheral blood monocyte surface , suggesting that these molecules may serve as profibrinolytic plasminogen receptors on monocytes . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Both immunization induced antibodies and 7 / 9 autoantibodies from patient sera inhibited the binding of plasminogen to alpha enolase . ^^^ Anti alpha enolase autoantibodies isolated from patient sera recognize the membrane associated form of the enzyme and / or interfere with its receptor function , thus inhibiting the binding of plasminogen . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Amino acid sequencing of tryptic peptides originating from the 59 , 56 and 43 kDa proteins , isolated as putative plg binding proteins , identified them as staphylococcal inosine 5 ' monophosphate dehydrogenase , alpha enolase , and ribonucleotide reductase subunit 2 , respectively . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Purification of native alpha enolase from Streptococcus pneumoniae that binds plasminogen and is immunogenic . ^^^ The plasminogen binding activity of alpha enolase was examined by Western blot , which showed that purified alpha enolase was able to bind human plasminogen . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha Enolase . ^^^ In the present study we have examined the role of alpha enolase in plasminogen activation on the cell surface . ^^^ We produced and characterized a monoclonal antibody ( MAb ) 11G1 against purified alpha enolase , which abrogated about 90 % of cell dependent plasminogen activation by either uPA or tPA on leukocytoid cell lines of different lineages : B lymphocytic , T lymphocytic , granulocytic , and monocytic cells . ^^^ These data suggest that , although leukocytic cells display several molecules that bind plasminogen , alpha enolase is responsible for the majority of the promotion of plasminogen activation on the surfaces of leukocytic cells . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning of an alpha enolase from the human filarial parasite Onchocerca volvulus that binds human plasminogen . ^^^ The plasminogen binding property of O . volvulus alpha enolase may support plasmin mediated proteolysis including degradation of host ' s extracellular matrix thereby promoting the migration of larval stages through tissues . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Plasminogen binds to surface displayed pneumococcal alpha enolase ( Eno ) and is subsequently activated to the serine protease plasmin by host derived tissue plasminogen activator ( tPA ) or urokinase ( uPA ) . ^^^ Pneumococcal mutants expressing alpha enolase with amino acid substitutions in the internal binding motif showed a substantially reduced plasminogen binding activity . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Plasmin generation dependent on alpha enolase type plasminogen receptor is required for myogenesis . ^^^ Alpha enolase constitutes a receptor for plasminogen on several leukocyte cell types , serving to localize and promote plasminogen activation pericellularly . ^^^ This study constitutes the first indication of alpha enolase type plasminogen receptor as an important component of skeletal myogenesis , by concentrating and enhancing plasmin generation on the cell surface . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Alpha enolase comes muscling in on plasminogen activation . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Alpha enolase ( SEN ) is a strong plasminogen binding protein on the surface of group A streptococci ( GAS ) . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Annexin 2 and alpha enolase have been reported to be plasminogen receptors , but we did not demonstrate a role for these proteins in conversion of plasminogen to AS4 . 5 . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| We report the crystal structure of alpha enolase from Streptococcus pneumoniae at 2 . 0A resolution , the first structure both of a plasminogen binding and of an octameric alpha enolase . ^^^ The nine residue plasminogen binding motif , by contrast , is exposed on the octamer surface revealing this as the primary site of interaction between alpha enolase and plasminogen . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Streptococcus mutans surface alpha enolase binds salivary mucin MG 2 and human plasminogen . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Alpha enolase plasminogen receptor in myogenesis . ^^^ Alpha enolase , although traditionally considered a glycolytic enzyme , constitutes a receptor for plasminogen on several cell types , serving to localize and promote plasminogen activation pericellularly . ^^^ However , the implication of alpha enolase plasminogen receptor in myogenesis had never been investigated . ^^^ This review focuses on the recently reported expression and function of alpha enolase plasminogen receptor during myogenesis . ^^^ Skeletal myoblasts express alpha enolase plasminogen receptor , being its expression greatly induced during the differentiation process in vitro . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The glycolytic enzyme alpha enolase represents one of the nonclassical cell surface plasminogen binding proteins of Streptococcus pneumoniae . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| On the surface of the decomposing spheroids , activation of the major extracellular proteolytic cascade , plasminogen activation , associated with surface exposure of alpha enolase , took place . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The gene structure of tetranectin , a plasminogen binding protein . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The alpha isoform of enolase possesses a carboxy terminal lysine and is a prominent plasminogen binding protein of cells . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| A novel plasminogen binding protein has been isolated from human plasma utilizing plasminogen Sepharose affinity chromatography . ^^^ The residues critical for catalysis and zinc and substrate binding of carboxypeptidase A and B are conserved in the Mr 60 , 000 plasminogen binding protein . ^^^ We propose that the Mr 60 , 000 plasminogen binding protein isolated here is a novel human plasma carboxypeptidase B and that it be designated pCPB . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| A plasminogen binding protein ( histidine rich glycoprotein ) , plasmin inhibitors and activator inhibitors appear to contribute to the regulation of the initial phase of fibrinolysis . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Tetranectin is a recently discovered plasminogen binding protein from human plasma , with a known primary structure . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| In this investigation , the principal plasminogen binding protein was identified in the plasma membrane fraction of rat hepatocytes . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Complement component C 7 is a plasminogen binding protein . ^^^ Among the terminal complement components C5b6 , C 7 , C 8 , and C 9 , the nonenzymatic component C 7 is a plasminogen binding protein . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Plasminogen binding protein associated with the plasma membrane of cultured embryonic rat neocortical neurons . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| PAM , a novel plasminogen binding protein from Streptococcus pyogenes . ^^^ Indeed , a 42 kDa plasminogen binding protein was solubilized . ^^^ When cloned and expressed in Escherichia coli , a resulting fragment encoded a 43 kDa plasminogen binding protein . ^^^ Nucleotide sequence determination of the gene fragment revealed an open reading frame encoding a polypeptide of 43 , 580 Da , which matched the amino terminal amino acid sequence of the plasminogen binding protein extracted from M 53 streptococci . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Cytokeratin 8 ( CK 8 ) has been identified as a plasminogen binding protein expressed on the external surfaces of hepatocytes and breast carcinoma cells [ Hembrough , Vasudevan , Allietta , Glass and Gonias ( 1995 ) J . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrated that CK 8 is the major plasminogen binding protein in plasma membrane fractions isolated from three breast cancer cell lines , BT 20 , MCF 7 , and MDA MB 157 . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Purification and characterisation of a plasminogen binding protein from Haemophilus influenzae . ^^^ In the present work we describe the purification and characterization of a plasminogen binding protein from Haemophilus influenzae ( strain HI 23459 ) . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Histidine proline rich glycoprotein ( HPRG ) , also known as histidine rich glycoprotein , is a major plasminogen binding protein . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Crystal structure of tetranectin , a trimeric plasminogen binding protein with an alpha helical coiled coil . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Isolation , cloning , and expression of a 70 kilodalton plasminogen binding protein of Borrelia burgdorferi . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Amino terminal sequencing coupled with peptide mass fingerprinting and immunologic analyses identified the plasminogen binding protein as annexin 2 and the angiostatin binding protein as the alpha / beta subunits of ATP synthase . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The functional consequence of up regulating the lysine binding property of K 2 ( Pg ) was explored , as reflected by its ability to interact with an internal sequence of a plasminogen binding protein ( PAM ) on the surface of group A streptococci . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| The chemokine macrophage inflammatory protein ( MIP ) 2alpha was identified as a plasminogen binding protein by phage display analysis . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Affinity chromatography assays revealed a plasminogen binding protein of 58 . 9 kDa in water extracts of surface proteins . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Purification , cloning , and characterization of a profibrinolytic plasminogen binding protein , TIP49a . ^^^ Antibodies against rat TIP49a recognized the plasminogen binding protein on two dimensional Western blots of U 937 cell membranes . ^^^ These results suggest that TIP49a is a previously unrecognized plasminogen binding protein on the U 937 cell surface . . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| These studies identified the plasminogen binding protein annexin 2 on the surface of macrophages and determined its role in their ability to degrade and migrate through extracellular matrices . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Here we address the issue of whether the plasminogen binding protein , p 11 , plays a significant role in this process . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Streptococcal surface enolase ( SEN ) is a major plasminogen binding protein of group A streptococci . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Human plasminogen binding protein tetranectin : crystallization and preliminary 10 ray analysis of the C type lectin CRD and the full length protein . ^^^ The recombinant human plasminogen binding protein tetranectin ( TN ) and the C type lectin CRD of this protein ( TN 3 ) have been crystallized . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Using two methods , ELISA and surface plasmon resonance , we demonstrate that indeed OspC is a plasminogen binding protein . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Enolase represents one of the anchorless surface proteins of Streptococcus pneumoniae and has previously been identified as a plasminogen binding protein , endowing this pathogen with host proteolytic activity . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| This study compared plasminogen plasma activity , protein C and protein S plasma activities , fibrinogen and fibrin degradation products ( FGDP and FBDP , respectively ) , von Willebrand factor antigen ( vWF Ag ) , prothrombin fragment F1 . 2 , thrombin antithrombin complexes ( TAT ) , tissue plasminogen activator ( tPA ) , and plasminogen activator inhibitor ( PAI ) in 16 patients with PPH and in 16 healthy volunteers . ^^^ Proteins C and S , FGDP , FBDP , and plasminogen levels as well as plasma concentrations of prothrombin fragment F1 . 2 and TAT were normal in the 16 patients with PPH . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| Plasma levels of plasminogen activator inhibitor type 1 ( PAI 1 ) , tissue type plasminogen activator ( t PA ) , fibrinogen , thrombin antithrombin ( TAT ) complexes , and prothrombin fragments ( F1 . 2 ) were measured at baseline and after standardized venous occlusion ( VO ) in patients with PPH ( 24 female , 9 male ) . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that endothelial mediators , produced in excess in PPH patients , may stimulate migrating mononuclear cells and thereby modulate alveolar macrophage function ; in particular , the plasminogen activation system . ^^^ Compared with controls , PPH patients had elevated plasma levels of tissue type plasminogen activator ( 15 . 6 + / 9 . 9 versus 5 . 5 + / 3 ng / ml ) and plasminogen activator inhibitor 1 ( 27 . 8 + / 23 versus 16 . 4 + / 12 ng / ml ) . ^^^ Dissociation constants ( K ( d ) ) for binding of scu PA and plasminogen to alveolar macrophages were similar in both PPH ( 4 . 7 + / 1 . 5 and 0 . 88 + / 0 . 3 micromol / l , respectively ) and control ( 6 . 7 + / 0 . 1 and 1 . 02 + / 0 . 12 micromol / l , respectively ) groups . ^^^ |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06733 and P00747 |
Pubmed |
SVM Score :0.0 |
| NA |
|