| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| CD 3 zeta dependence of the CD 2 pathway of activation in T lymphocytes and natural killer cells . ^^^ Because the TCR on T lymphocytes and the CD 16 low affinity IgG Fc receptor ( Fc gamma receptor type 3 ) complex on NK cells share a common CD 3 zeta subunit , we reasoned that CD 3 zeta may be critical for CD 2 signaling in T lymphocytes and NK cells . ^^^ Here we show that transfection of a cDNA encoding a transmembrane form of CD 16 into TCR variants of the Jurkat T cell line results in CD 16 expression in association with endogenous CD 3 zeta homodimers and restores CD 2 signaling function . ^^^ To test directly the role of CD 3 zeta in CD 2 triggering , we then transfected human CD 2 into each of two murine T T hybridomas that express TCRs containing either a full length CD 3 zeta subunit or a truncated CD 3 zeta subunit incapable of transducing signals . ^^^ Despite expression of equivalent surface levels of TCR , CD 2 mediated signaling is seen only in the T cells containing wild type CD 3 zeta . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| The CD 3 zeta cytoplasmic domain mediates CD 2 induced T cell activation . ^^^ Given the importance of CD 3 zeta in TCR signaling , we have directly examined the ability of the CD 3 zeta cytoplasmic domain to couple CD 2 to intracellular signal transduction pathways . ^^^ These data directly establish the CD 3 zeta cytoplasmic domain as a necessary and sufficient component of the CD 3 Ti complex involved in T lymphocyte activation through CD 2 . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| We thus conclude that there is no clear evidence for potent signaling via CD 2 on cells that lack a TCR complex and that TCR zeta chain expressed at the cell surface is not sufficient to potentiate signaling via CD 2 as measured by an increase in [ Ca2+ ] i . . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| In these mast cells , CD 2 signaling machinery does not require CD 3 zeta / eta and may be linked to the Fc epsilon RI gamma subunit . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| Using antiphosphotyrosine antibody to detect tyrosine phosphorylation of cellular substrates , we demonstrate that mAb stimulation of either CD 3 or CD 2 results in tyrosine phosphorylation of the TCR zeta chain and 135 and 100 kDa proteins . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| Using TIA 2 as a TCR zeta specific reagent , we show that whole cell expression of this TCR subunit is dramatically reduced following exposure to mAb reactive with CD 3 . mAb reactive with activating epitopes of CD 2 were also capable of down modulating the expression of TCR zeta , but to a lesser degree . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| We show that the expression of CD 4 and CD 8 on thymocyte subpopulations with low CD 3 ( CD3L ) and high CD 3 ( CD3H ) is regulated by activation through the CD 2 molecule and perturbation of the CD 3 T cell receptor complex ( CD 3 Ti ) . ^^^ Treatment with anti T 11 ( 2 ) and anti T 11 ( 3 ) antibodies ( CD 2 activation pathway ) resulted in partial down regulation of CD 4 but not CD 8 surface expression on both CD3L and CD3H thymocytes . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| Importantly , this CD 2 induced tyrosine phosphorylation of p 63 can also occur in the absence of the CD 3 zeta chain membrane expression , and is also distinct from the protein tyrosine kinases p56lck and p59fyn . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| During the first 16 wk of gestation , we observed developmentally regulated expression of CD 2 and CD 8 beta ( appearing at 9 . 5 wk ) , CD1a , b , and c molecules ( CD1b , then CD1c , then CD1a ) , TCR molecules ( TCR beta , then TCR delta ) , CD45RA and CD45RO isoforms , CD 28 ( 10 wk ) , CD 3 zeta ( 12 13 wk ) , and CD 6 ( 12 , 75 wk ) . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| Five of 35 CD 3 DGL clones expressed cytoplasmic CD 3 zeta in the absence of expression of CD 2 , CD 16 or the p 58 molecule recognized by HP3E4 . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| CD 3 gamma , CD 3 delta , and CD 3 zeta mRNA in adult human marrow hematopoietic progenitors correlates with surface CD 2 and CD 7 expression . ^^^ Expression of both CD 7 and CD 2 on CD34+ cells is associated with not only CD 3 zeta gene transcription but also CD 3 gamma and CD 3 delta , a pattern found in T cells but not mature NK cells . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| With the use of different Jurkat cell mutants it was demonstrated that CD 2 mediated activation of EMT required expression of LCK , but not require surface expression of the CD 3 zeta chain . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| In particular , anti CD3epsilon caused transient down modulation of the TCRbeta receptor subunit and the TCRzeta signaling module , and this pattern was enhanced and prolonged by anti CD 2 . ^^^ Anti TCRbeta caused persistent TCRzeta modulation but no TCRbeta modulation , and anti CD 2 did not influence this pattern . ^^^ CONCLUSIONS : These results suggest that , although full T cell activation is not required for the induction of tolerance by anti CD 2 plus anti CD3epsilon mAb , a signal transduction pathway that is associated with TCRbeta and TCRzeta expression , and , specifically , is perturbed by mAb binding of the CD3epsilon epitope , is critical . . ^^^ |
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| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P06729 and P20963 |
Pubmed |
SVM Score :0.0 |
| NA |
|